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MX2023008629A - Nuevas nucleasas modificadas y quimericas. - Google Patents

Nuevas nucleasas modificadas y quimericas.

Info

Publication number
MX2023008629A
MX2023008629A MX2023008629A MX2023008629A MX2023008629A MX 2023008629 A MX2023008629 A MX 2023008629A MX 2023008629 A MX2023008629 A MX 2023008629A MX 2023008629 A MX2023008629 A MX 2023008629A MX 2023008629 A MX2023008629 A MX 2023008629A
Authority
MX
Mexico
Prior art keywords
nucleases
chimeric
new modified
chimeric nucleases
genetically modified
Prior art date
Application number
MX2023008629A
Other languages
English (en)
Inventor
Christopher Brown
Cristina Butterfield
Greg Cost
Rebecca Lamothe
Jyun-Liang Lin
Brian C Thomas
Morayma M Temoche-Diaz
Alan Brooks
Original Assignee
Metagenomi Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metagenomi Inc filed Critical Metagenomi Inc
Publication of MX2023008629A publication Critical patent/MX2023008629A/es

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03015—(S)-2-Hydroxy-acid oxidase (1.1.3.15)
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
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    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

En la presente descripción se describen nucleasas modificadas genéticamente y sistemas de nucleasas, que incluyen nucleasas quiméricas y sistemas de nucleasas quiméricas. Las nucleasas quiméricas y modificadas genéticamente descritas en la presente descripción incluyen nucleasa guiada por ácido nucleico. Adicionalmente, en la presente descripción se describen métodos para generar nucleasas modificadas genéticamente y métodos para usarlas.
MX2023008629A 2021-01-22 2022-01-21 Nuevas nucleasas modificadas y quimericas. MX2023008629A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163140620P 2021-01-22 2021-01-22
US202163237484P 2021-08-26 2021-08-26
PCT/US2022/013396 WO2022159758A1 (en) 2021-01-22 2022-01-21 Novel engineered and chimeric nucleases

Publications (1)

Publication Number Publication Date
MX2023008629A true MX2023008629A (es) 2023-10-25

Family

ID=82549895

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023008629A MX2023008629A (es) 2021-01-22 2022-01-21 Nuevas nucleasas modificadas y quimericas.

Country Status (10)

Country Link
US (1) US20230416710A1 (es)
EP (1) EP4281554A4 (es)
JP (1) JP2024504981A (es)
KR (1) KR20230134543A (es)
AU (1) AU2022210762A1 (es)
BR (1) BR112023014719A2 (es)
CA (1) CA3205865A1 (es)
GB (1) GB2610711B (es)
MX (1) MX2023008629A (es)
WO (1) WO2022159758A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7605852B2 (ja) 2020-03-06 2024-12-24 メタゲノミ，インク． クラスｉｉのｖ型ｃｒｉｓｐｒ系
AU2022335499A1 (en) 2021-08-27 2024-02-22 Metagenomi, Inc. Enzymes with ruvc domains
EP4532707A2 (en) * 2022-05-25 2025-04-09 Metagenomi, Inc. Supplementation of liver enzyme expression
WO2024026499A2 (en) * 2022-07-29 2024-02-01 Metagenomi, Inc. Class ii, type v crispr systems
WO2024156085A1 (en) * 2023-01-27 2024-08-02 Syngenta Crop Protection Ag Mb2cas12a variants with enhanced efficiency
AU2024230342A1 (en) * 2023-03-01 2025-10-16 Metagenomi, Inc. Enzymes with ruvc domains
WO2025059585A1 (en) * 2023-09-13 2025-03-20 Metagenomi, Inc. Engineered and chimeric nucleases

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3138911B1 (en) * 2012-12-06 2018-12-05 Sigma Aldrich Co. LLC Crispr-based genome modification and regulation
EP3089989B1 (en) * 2013-12-31 2020-06-24 The Regents of The University of California Cas9 crystals and methods of use thereof
WO2016205759A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
EP3679137A4 (en) * 2017-09-07 2021-06-02 The Board of Trustees of the Leland Stanford Junior University NUCLEASE SYSTEMS FOR GENE ENGINEERING
WO2020123887A2 (en) * 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing
CN113728098A (zh) * 2019-02-14 2021-11-30 宏基因组学知识产权技术有限责任公司 具有ruvc结构域的酶
US10982200B2 (en) * 2019-02-14 2021-04-20 Metagenomi Ip Technologies, Llc Enzymes with RuvC domains
AU2021267379A1 (en) * 2020-05-08 2022-12-08 Metagenomi, Inc. Enzymes with RuvC domains
WO2022056324A1 (en) * 2020-09-11 2022-03-17 Metagenomi Ip Technologies, Llc Base editing enzymes
MX2023010969A (es) * 2021-03-19 2023-09-27 Metagenomi Inc Edicion multiplex con enzimas cas.
EP4426826A4 (en) * 2021-11-05 2025-08-20 Metagenomi Inc BASIC EDITION ENZYMES

Also Published As

Publication number Publication date
KR20230134543A (ko) 2023-09-21
BR112023014719A2 (pt) 2023-12-05
GB202215621D0 (en) 2022-12-07
US20230416710A1 (en) 2023-12-28
CA3205865A1 (en) 2022-07-28
GB2610711B (en) 2023-08-02
WO2022159758A1 (en) 2022-07-28
AU2022210762A1 (en) 2023-08-24
EP4281554A1 (en) 2023-11-29
EP4281554A4 (en) 2025-06-18
AU2022210762A9 (en) 2024-09-19
GB2610711A (en) 2023-03-15
JP2024504981A (ja) 2024-02-02

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