MX2018007203A - Sistema de transposon, kit que comprende el mismo, y sus usos. - Google Patents
Sistema de transposon, kit que comprende el mismo, y sus usos.Info
- Publication number
- MX2018007203A MX2018007203A MX2018007203A MX2018007203A MX2018007203A MX 2018007203 A MX2018007203 A MX 2018007203A MX 2018007203 A MX2018007203 A MX 2018007203A MX 2018007203 A MX2018007203 A MX 2018007203A MX 2018007203 A MX2018007203 A MX 2018007203A
- Authority
- MX
- Mexico
- Prior art keywords
- transposon system
- kit
- exogenous dna
- cell
- same
- Prior art date
Links
- 108091029865 Exogenous DNA Proteins 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 2
- 210000002865 immune cell Anatomy 0.000 abstract 2
- 108091081062 Repeated sequence (DNA) Proteins 0.000 abstract 1
- 102000008579 Transposases Human genes 0.000 abstract 1
- 108010020764 Transposases Proteins 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A61P35/02—Antineoplastic agents specific for leukemia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Hematology (AREA)
- Pulmonology (AREA)
- Pain & Pain Management (AREA)
- Rheumatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Un método in vitro para integrar una secuencia de ADN exógeno en el genoma de una célula usando un sistema de transposón. El sistema de transposón incluye un primer vector que lleva secuencias de repetición invertida y un ADN exógeno, y un segundo vector que expresa una transposasa. También se proporciona un kit que incluye el sistema de transposón para integrar un ADN exógeno en el genoma de una célula. Se describe un método para tratar un sujeto que incluye diseñar una célula inmune para transportar una secuencia de ADN exógeno usando el método y/o el kit descrito anteriormente y administrar una cantidad efectiva de la célula inmune modificada al sujeto.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562267270P | 2015-12-14 | 2015-12-14 | |
| PCT/CN2016/109510 WO2017101749A1 (en) | 2015-12-14 | 2016-12-12 | Transposon system, kit comprising the same, and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2018007203A true MX2018007203A (es) | 2018-11-12 |
| MX380289B MX380289B (es) | 2025-03-12 |
Family
ID=59055731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2018007203A MX380289B (es) | 2015-12-14 | 2016-12-12 | Sistema de transposón, kit que comprende el mismo, y sus usos. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US11345926B2 (es) |
| JP (1) | JP6956416B2 (es) |
| KR (1) | KR102249982B1 (es) |
| CN (1) | CN108474005B (es) |
| AU (1) | AU2016373365B2 (es) |
| CA (1) | CA3004665C (es) |
| MX (1) | MX380289B (es) |
| MY (1) | MY184126A (es) |
| NZ (1) | NZ742068A (es) |
| TW (1) | TWI609960B (es) |
| WO (1) | WO2017101749A1 (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
| US20190119636A1 (en) | 2017-10-23 | 2019-04-25 | Poseida Therapeutics, Inc. | Modified stem cell memory t cells, methods of making and methods of using same |
| KR102523318B1 (ko) * | 2016-12-16 | 2023-04-18 | 비-모젠 바이오테크놀로지스, 인크. | 증진된 ｈＡＴ 패밀리 트랜스포존 매개 유전자 전달 및 연관된 조성물, 시스템, 및 방법 |
| US11278570B2 (en) | 2016-12-16 | 2022-03-22 | B-Mogen Biotechnologies, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
| CA3104288A1 (en) | 2018-06-21 | 2019-12-26 | B-Mogen Biotechnologies, Inc. | Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods |
| CA3124103A1 (en) * | 2018-12-20 | 2020-06-25 | Poseida Therapeutics, Inc. | Nanotransposon compositions and methods of use |
| CN114026240A (zh) * | 2019-06-11 | 2022-02-08 | 庞培法布拉大学 | 靶向基因编辑构建体及其使用方法 |
| JP7693253B2 (ja) * | 2021-07-09 | 2025-06-17 | ネオジェンティーシ― コーポレーション | トランスポゾンシステムおよびその用途 |
| WO2023060589A1 (zh) * | 2021-10-15 | 2023-04-20 | 深圳市深研生物科技有限公司 | 多转座子系统 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6962810B2 (en) * | 2000-10-31 | 2005-11-08 | University Of Notre Dame Du Lac | Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector piggyBac |
| CN101297031B (zh) * | 2005-05-14 | 2013-06-05 | 复旦大学 | 在脊椎动物中作为遗传操作和分析工具的piggyBac |
| US20090042297A1 (en) * | 2007-06-01 | 2009-02-12 | George Jr Alfred L | Piggybac transposon-based vectors and methods of nucleic acid integration |
| KR20080041146A (ko) * | 2007-12-13 | 2008-05-09 | 푸단 유니버시티 | 척추동물에서의 유전자 조작 및 분석을 위한 도구로서의피기백 |
| US20110311506A1 (en) * | 2009-02-25 | 2011-12-22 | The Johns Hopkins University | Piggybac transposon variants and methods of use |
| DE102011118018B4 (de) | 2011-10-25 | 2017-10-26 | Plasmidfactory Gmbh & Co. Kg | Minicircles mit Transpositionskassetten und ihre Verwendung zur Transformation von Zellen |
| EP3513802B1 (en) * | 2012-10-26 | 2023-11-29 | Vrije Universiteit Brussel | Vector for liver-directed gene therapy of hemophilia and methods and use thereof |
| WO2014081729A1 (en) * | 2012-11-20 | 2014-05-30 | J.R. Simplot Company | Tal-mediated transfer dna insertion |
| CN102943092B (zh) * | 2012-11-20 | 2014-12-10 | 西北农林科技大学 | 一种通用型PiggyBac转座子转基因载体及其制备方法 |
| CN104342453A (zh) * | 2013-08-06 | 2015-02-11 | 深圳先进技术研究院 | 含基因工程抗体基因表达盒的微环dna重组母质粒、含该表达盒的微环dna及应用 |
| AU2015234242B2 (en) | 2014-03-21 | 2020-09-24 | Children's Medical Research Institute | Stable gene transfer to proliferating cells |
| EP3670660A1 (en) | 2014-04-09 | 2020-06-24 | Dna Twopointo Inc. | Enhanced nucleic acid constructs for eukaryotic gene expression |
| KR102463529B1 (ko) | 2014-04-10 | 2022-11-07 | 시애틀 칠드런즈 호스피탈 디/비/에이 시애틀 칠드런즈 리서치 인스티튜트 | 약물 관련 트랜스진 발현 |
-
2016
- 2016-12-12 CN CN201680071463.4A patent/CN108474005B/zh active Active
- 2016-12-12 TW TW105141119A patent/TWI609960B/zh active
- 2016-12-12 US US15/766,707 patent/US11345926B2/en active Active
- 2016-12-12 MX MX2018007203A patent/MX380289B/es unknown
- 2016-12-12 CA CA3004665A patent/CA3004665C/en active Active
- 2016-12-12 WO PCT/CN2016/109510 patent/WO2017101749A1/en not_active Ceased
- 2016-12-12 JP JP2018524760A patent/JP6956416B2/ja active Active
- 2016-12-12 NZ NZ742068A patent/NZ742068A/en unknown
- 2016-12-12 AU AU2016373365A patent/AU2016373365B2/en active Active
- 2016-12-12 KR KR1020187017078A patent/KR102249982B1/ko active Active
- 2016-12-12 MY MYPI2018701786A patent/MY184126A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA3004665A1 (en) | 2017-06-22 |
| KR102249982B1 (ko) | 2021-05-11 |
| KR20180092989A (ko) | 2018-08-20 |
| MY184126A (en) | 2021-03-19 |
| US20180320196A1 (en) | 2018-11-08 |
| TW201720926A (zh) | 2017-06-16 |
| JP6956416B2 (ja) | 2021-11-02 |
| CN108474005B (zh) | 2022-10-21 |
| US11345926B2 (en) | 2022-05-31 |
| JP2019503653A (ja) | 2019-02-14 |
| TWI609960B (zh) | 2018-01-01 |
| WO2017101749A1 (en) | 2017-06-22 |
| AU2016373365A1 (en) | 2018-05-17 |
| AU2016373365B2 (en) | 2021-07-08 |
| NZ742068A (en) | 2023-07-28 |
| CN108474005A (zh) | 2018-08-31 |
| MX380289B (es) | 2025-03-12 |
| CA3004665C (en) | 2021-08-24 |
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