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MX2016004140A - Analisis de mutacion que usa temperatura de fusion y bases universales. - Google Patents

Analisis de mutacion que usa temperatura de fusion y bases universales.

Info

Publication number
MX2016004140A
MX2016004140A MX2016004140A MX2016004140A MX2016004140A MX 2016004140 A MX2016004140 A MX 2016004140A MX 2016004140 A MX2016004140 A MX 2016004140A MX 2016004140 A MX2016004140 A MX 2016004140A MX 2016004140 A MX2016004140 A MX 2016004140A
Authority
MX
Mexico
Prior art keywords
probe
target sequence
variants
melting temperature
mutation analysis
Prior art date
Application number
MX2016004140A
Other languages
English (en)
Inventor
Ben Cobb
Original Assignee
Epistem Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epistem Ltd filed Critical Epistem Ltd
Publication of MX2016004140A publication Critical patent/MX2016004140A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método y una sonda de oligonucleótidos se describen para determinar la presencia o ausencia de alelos mutantes en un sitio genómico. La zona se une a diferentes alelos de una secuencia objetivo con diferentes temperaturas de fusión (Tm). El método determina la Tm de la sonda cuando se hibridiza a la secuencia objetivo para establecer si un ácido nucleico de variante tal un alelo mutante está presente o ausente en la secuencia objetivo. Puede haber variantes en una secuencia objetivo que no son de interés, por ejemplo, mutaciones fenotípicamente silenciosas. Para asegurar que estas variantes no influencian la Tm de la sonda, la sonda contiene sitios de base universal en donde se presentan variantes que no son de interés.
MX2016004140A 2013-10-01 2014-09-30 Analisis de mutacion que usa temperatura de fusion y bases universales. MX2016004140A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1317355.4A GB201317355D0 (en) 2013-10-01 2013-10-01 Mutation Analysis
PCT/GB2014/052939 WO2015049496A1 (en) 2013-10-01 2014-09-30 Mutation analysis using melting temperature and universal bases

Publications (1)

Publication Number Publication Date
MX2016004140A true MX2016004140A (es) 2016-06-06

Family

ID=49585117

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016004140A MX2016004140A (es) 2013-10-01 2014-09-30 Analisis de mutacion que usa temperatura de fusion y bases universales.

Country Status (9)

Country Link
US (1) US10280452B2 (es)
EP (1) EP3052648B1 (es)
JP (1) JP6461114B2 (es)
KR (1) KR20160057486A (es)
CN (1) CN105916999B (es)
BR (1) BR112016007059A2 (es)
GB (1) GB201317355D0 (es)
MX (1) MX2016004140A (es)
WO (1) WO2015049496A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105368943B (zh) * 2015-11-21 2018-10-16 中国人民解放军第三〇九医院 一种用于鉴定分枝杆菌菌种的试剂盒及方法
CN109868309B (zh) * 2019-03-05 2022-11-11 苏州恩可医药科技有限公司 基于通用碱基替换插入的单链dna扩增方法
WO2022092012A1 (ja) * 2020-10-30 2022-05-05 東洋紡株式会社 複数の標的核酸の同時検出方法
CN116287107A (zh) * 2021-12-21 2023-06-23 广东菲鹏生物有限公司 一种核酸探针及其使用方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6511809B2 (en) * 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US7348146B2 (en) * 2003-10-02 2008-03-25 Epoch Biosciences, Inc. Single nucleotide polymorphism analysis of highly polymorphic target sequences
WO2006095941A1 (en) * 2005-03-05 2006-09-14 Seegene, Inc. Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
EP1899364B2 (en) * 2005-05-17 2024-10-30 University of Connecticut Compositions and methods for immunomodulation in an organism
US9677123B2 (en) * 2006-03-15 2017-06-13 Siemens Healthcare Diagnostics Inc. Degenerate nucleobase analogs
EP2038426A2 (en) * 2006-06-16 2009-03-25 Sequenom, Inc. Methods and compositions for the amplification, detection and quantification of nucleic acid from a sample
FI121428B (fi) 2008-01-17 2010-11-15 Mobidiag Oy Laajakirjoisia alukkeita, testipakkaus ja menetelmä bakteerilajien havaitsemiseksi ja tunnistamiseksi
GB201100150D0 (en) * 2011-01-06 2011-02-23 Epistem Ltd Mutational analysis
GB201116131D0 (en) * 2011-09-19 2011-11-02 Epistem Ltd Probe
HK1217517A1 (zh) * 2013-01-23 2017-01-13 Brandeis University 用於提高pcr准确度的试剂

Also Published As

Publication number Publication date
CN105916999A (zh) 2016-08-31
EP3052648A1 (en) 2016-08-10
JP6461114B2 (ja) 2019-01-30
BR112016007059A2 (pt) 2017-09-19
GB201317355D0 (en) 2013-11-13
EP3052648B1 (en) 2018-06-27
CN105916999B (zh) 2020-02-21
US20160237481A1 (en) 2016-08-18
WO2015049496A1 (en) 2015-04-09
JP2016533171A (ja) 2016-10-27
KR20160057486A (ko) 2016-05-23
US10280452B2 (en) 2019-05-07

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