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MX2008011669A - Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina. - Google Patents

Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.

Info

Publication number
MX2008011669A
MX2008011669A MX2008011669A MX2008011669A MX2008011669A MX 2008011669 A MX2008011669 A MX 2008011669A MX 2008011669 A MX2008011669 A MX 2008011669A MX 2008011669 A MX2008011669 A MX 2008011669A MX 2008011669 A MX2008011669 A MX 2008011669A
Authority
MX
Mexico
Prior art keywords
amino acid
unnatural amino
phenylselenocysteine
proteins
novel
Prior art date
Application number
MX2008011669A
Other languages
English (en)
Inventor
Peter G Schultz
Jiangyun Wang
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of MX2008011669A publication Critical patent/MX2008011669A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La invención es concerniente con pares ortogonales de tARN y aminoacil-tARN sintetasas que pueden incorporar el aminoácido no natural fenilselenocisteína a proteínas producidas en células huésped eubacterianas tales como E. coli. La invención proporciona, por ejemplo pero no limitado a, nuevas aminoacil-tARN sintetasas ortogonales, polinucleótidos que codifican las nuevas moléculas de sintetasa, métodos para identificar y enmascarar las nuevas sintetasas, métodos para producir proteínas que contienen el aminoácido no natural fenilselenocisteína y sistemas de traducción. La invención proporciona además métodos para producir proteínas modificadas (por ejemplo, proteínas lapidadas) por medio de la modificación apuntada del residuo de fenilselenocisteína en una proteína.
MX2008011669A 2006-03-16 2007-03-13 Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina. MX2008011669A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US78327206P 2006-03-16 2006-03-16
US86145606P 2006-11-28 2006-11-28
PCT/US2007/006382 WO2007109035A2 (en) 2006-03-16 2007-03-13 Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Publications (1)

Publication Number Publication Date
MX2008011669A true MX2008011669A (es) 2008-09-22

Family

ID=38522926

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2008011669A MX2008011669A (es) 2006-03-16 2007-03-13 Expresion programada geneticamente de proteinas que contienen el aminoacido no natural fenilselenocisteina.

Country Status (12)

Country Link
US (3) US8217145B2 (es)
EP (1) EP1994165B1 (es)
JP (1) JP5249194B2 (es)
KR (1) KR20080113226A (es)
CN (1) CN101405401B (es)
AU (1) AU2007227592B2 (es)
BR (1) BRPI0709603A2 (es)
CA (1) CA2638772A1 (es)
IL (1) IL193722A0 (es)
MX (1) MX2008011669A (es)
RU (1) RU2464315C2 (es)
WO (1) WO2007109035A2 (es)

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SG160203A1 (en) * 2002-10-16 2010-04-29 Scripps Research Inst Glycoprotein synthesis
CN101223272B (zh) 2003-04-17 2013-04-03 斯克利普斯研究院 扩展真核生物遗传密码
EP1649004A4 (en) 2003-07-07 2008-04-09 Scripps Research Inst COMPOSITIONS WITH PAIRS OF ORTHOGONAL LYSYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF
EP1807515A4 (en) * 2004-10-27 2008-06-18 Scripps Research Inst Orthogonal translation components for the in vivo incorporation of unnatural amino acids
AU2007223830A1 (en) * 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
EP1994165B1 (en) 2006-03-16 2014-11-05 The Scripps Research Institute Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
AU2007267950B2 (en) * 2006-05-23 2012-08-16 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
US8114627B2 (en) 2006-09-21 2012-02-14 The Scripps Research Institute Genetically programmed expression of selectively sulfated proteins in eubacteria
RU2009112104A (ru) 2006-10-18 2010-11-27 Зе Скрипс Ресеч Инститьют (Us) Генетическое встраивание не встречающихся в природе аминокислот в белки клеток млекопитающих
WO2009055616A2 (en) 2007-10-25 2009-04-30 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
US9249408B2 (en) * 2007-11-02 2016-02-02 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
AU2008335778B2 (en) * 2007-12-11 2014-04-10 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris
WO2009086497A2 (en) * 2007-12-28 2009-07-09 Ardell David H Genetic encryption
CA2710683A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
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US8637306B2 (en) 2008-12-10 2014-01-28 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US8658394B2 (en) * 2011-02-02 2014-02-25 Waterstone Pharmaceuticals Methods, cells and systems for incorporating non-canonical amino acids into proteins
CN103215235B (zh) * 2012-01-18 2014-11-05 中国科学院生物物理研究所 3-咪唑基酪氨酸翻译系统及其应用
CN103571804B (zh) * 2012-08-10 2015-08-12 中国科学院生物物理研究所 3-吡唑基酪氨酸翻译系统及其应用
KR102018863B1 (ko) 2012-10-12 2019-09-05 서트로 바이오파마, 인크. 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화
CN103820410B (zh) * 2012-11-16 2016-03-30 中国科学院生物物理研究所 3-甲硫酪氨酸翻译系统及其应用
CN104004723B (zh) * 2013-02-22 2016-08-17 中国科学院生物物理研究所 3,5-二氟代酪氨酸翻译系统及其应用
CN104059891B (zh) * 2013-03-22 2016-08-17 中国科学院生物物理研究所 8-羟基喹啉丙氨酸翻译系统及其应用
JP6822839B2 (ja) * 2013-09-13 2021-01-27 ザ・スクリップス・リサーチ・インスティテュート 修飾された治療剤、及びその組成物
US10039809B2 (en) 2013-12-18 2018-08-07 The California Institute For Biomedical Research Modified therapeutic agents, stapled peptide lipid conjugates, and compositions thereof
DK3458074T3 (da) * 2016-05-16 2024-07-29 Univ Texas COMPOSITIONS FOR THE DELIVERY OF tRNA AS NANOPARTICLES AND METHODS OF USE THEREWITH
KR102747205B1 (ko) 2016-12-29 2024-12-31 일루미나, 인코포레이티드 세포 구획 내의 생체분자에 대한 직교 접근 및 그의 태그부착을 위한 분석 시스템
CN114644581B (zh) * 2021-01-04 2024-07-16 北京惠大生物科技有限公司 含芳基硫酚或芳基硒酚经修饰的氨基酸、重组蛋白及其生物合成方法及应用
KR20240032010A (ko) 2021-06-09 2024-03-08 더 스크립스 리서치 인스티튜트 장기 지속형 이중 gip/glp-1 펩타이드 접합체 및 사용 방법
CN118389493B (zh) * 2024-05-10 2025-04-11 北京大学 一种定点修饰的工程化tRNA及其应用

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AU2007223830A1 (en) 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
EP1994165B1 (en) 2006-03-16 2014-11-05 The Scripps Research Institute Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Also Published As

Publication number Publication date
CN101405401A (zh) 2009-04-08
EP1994165A2 (en) 2008-11-26
WO2007109035A3 (en) 2008-10-16
EP1994165B1 (en) 2014-11-05
RU2008135114A (ru) 2010-04-27
US7790847B2 (en) 2010-09-07
JP5249194B2 (ja) 2013-07-31
WO2007109035A2 (en) 2007-09-27
US8394605B2 (en) 2013-03-12
CN101405401B (zh) 2013-03-27
US20070238152A1 (en) 2007-10-11
BRPI0709603A2 (pt) 2011-07-19
US20120282668A1 (en) 2012-11-08
CA2638772A1 (en) 2007-09-27
RU2464315C2 (ru) 2012-10-20
JP2009529877A (ja) 2009-08-27
IL193722A0 (en) 2011-08-01
US20090209000A1 (en) 2009-08-20
KR20080113226A (ko) 2008-12-29
AU2007227592A1 (en) 2007-09-27
US8217145B2 (en) 2012-07-10
AU2007227592B2 (en) 2011-11-24
EP1994165A4 (en) 2012-11-07

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