[go: up one dir, main page]

ZA200900787B - Zinc finger nuclease-mediated homologous recombination - Google Patents

Zinc finger nuclease-mediated homologous recombination

Info

Publication number
ZA200900787B
ZA200900787B ZA200900787A ZA200900787A ZA200900787B ZA 200900787 B ZA200900787 B ZA 200900787B ZA 200900787 A ZA200900787 A ZA 200900787A ZA 200900787 A ZA200900787 A ZA 200900787A ZA 200900787 B ZA200900787 B ZA 200900787B
Authority
ZA
South Africa
Prior art keywords
homologous recombination
zinc finger
finger nuclease
mediated homologous
mediated
Prior art date
Application number
ZA200900787A
Other languages
English (en)
Inventor
Qihua Cai
Jeffrey Miller
William Michael Ainley
Robbi Janette Garrison
Joseph F Petolino
Beth Cori Rubin-Wilson
Lisa Lynn Schulenberg
Andrew Frederick Worden
Original Assignee
Dow Agrosciences Llc
Sangamo Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc, Sangamo Biosciences Inc filed Critical Dow Agrosciences Llc
Publication of ZA200900787B publication Critical patent/ZA200900787B/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213Targeted insertion of genes into the plant genome by homologous recombination
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • C12N5/12Fused cells, e.g. hybridomas
    • C12N5/14Plant cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
ZA200900787A 2006-08-11 2007-08-09 Zinc finger nuclease-mediated homologous recombination ZA200900787B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US83714706P 2006-08-11 2006-08-11

Publications (1)

Publication Number Publication Date
ZA200900787B true ZA200900787B (en) 2010-05-26

Family

ID=38624400

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200900787A ZA200900787B (en) 2006-08-11 2007-08-09 Zinc finger nuclease-mediated homologous recombination

Country Status (19)

Country Link
US (2) US9428756B2 (xx)
EP (2) EP2049663B1 (xx)
JP (4) JP2010500029A (xx)
KR (1) KR101439568B1 (xx)
CN (2) CN101528924B (xx)
AR (3) AR062336A1 (xx)
AU (1) AU2007284748B2 (xx)
BR (1) BRPI0716427A2 (xx)
CA (1) CA2660485C (xx)
DK (1) DK2049663T3 (xx)
ES (1) ES2532002T3 (xx)
HK (1) HK1221251A1 (xx)
IL (1) IL196942A (xx)
PL (1) PL2049663T3 (xx)
SI (1) SI2049663T1 (xx)
TW (1) TWI424061B (xx)
UA (1) UA106193C2 (xx)
WO (1) WO2008021207A2 (xx)
ZA (1) ZA200900787B (xx)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003087341A2 (en) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
US7528643B2 (en) * 2003-02-12 2009-05-05 Semiconductor Energy Laboratory Co., Ltd. Semiconductor device, electronic device having the same, and driving method of the same
EP2092068B1 (en) * 2006-12-14 2014-10-08 Dow AgroSciences LLC Optimized non-canonical zinc finger proteins
WO2009006297A2 (en) 2007-06-29 2009-01-08 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
EP2188384B1 (en) * 2007-09-27 2015-07-15 Sangamo BioSciences, Inc. Rapid in vivo identification of biologically active nucleases
CN102586236B (zh) 2008-02-29 2014-10-15 孟山都技术公司 玉米植物事件mon87460以及用于其检测的组合物和方法
WO2009114321A2 (en) 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
WO2009131632A1 (en) * 2008-04-14 2009-10-29 Sangamo Biosciences, Inc. Linear donor constructs for targeted integration
KR20160015400A (ko) * 2008-08-22 2016-02-12 상가모 바이오사이언스 인코포레이티드 표적화된 단일가닥 분할 및 표적화된 통합을 위한 방법 및 조성물
TWI576432B (zh) * 2009-04-07 2017-04-01 陶氏農業科學公司 序列特異性核酸酶之奈米粒子媒介式遞送技術
IN2012DN02294A (xx) * 2009-08-19 2015-08-21 Dow Agrosciences Llc
US8598413B2 (en) 2009-08-19 2013-12-03 Dow AgroSciecnes, LLC. AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1
CN102639695B (zh) * 2009-10-26 2015-01-21 独立行政法人农业生物资源研究所 转基因植物细胞的制造方法
EP2319872A1 (en) 2009-11-04 2011-05-11 BASF Plant Science GmbH Amylopectin type starch with enhanced retrogradation stability
CN104928256A (zh) 2009-11-24 2015-09-23 陶氏益农公司 Aad-12事件416、相关的转基因大豆系及其事件特异性鉴定
EP2526112B1 (en) 2010-01-22 2018-10-17 Dow AgroSciences LLC Targeted genomic alteration
CN103025866A (zh) * 2010-03-22 2013-04-03 菲利普莫里斯生产公司 修饰植物中酶的活性
US9567573B2 (en) * 2010-04-26 2017-02-14 Sangamo Biosciences, Inc. Genome editing of a Rosa locus using nucleases
WO2012051343A1 (en) 2010-10-12 2012-04-19 The Children's Hospital Of Philadelphia Methods and compositions for treating hemophilia b
CA2820706C (en) 2010-12-03 2018-05-22 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
JP6111200B2 (ja) 2010-12-03 2017-04-05 ダウ アグロサイエンシィズ エルエルシー 除草剤耐性イベント8264.44.06.1のスタック、関連するトランスジェニックダイズ系統、およびその検出
CN102174649B (zh) * 2011-01-18 2013-06-12 陕西师范大学 快速检测锌指核酸酶介导基因定点整合的方法
PT2702160T (pt) 2011-04-27 2020-07-30 Amyris Inc Métodos para modificação genómica
EP2704554B1 (en) 2011-05-02 2019-04-10 The Board of Regents of the University of Nebraska Plants with useful traits and related methods
WO2012159079A1 (en) 2011-05-18 2012-11-22 The Parkinson's Institute Assay to determine lrrk2 activity in parkinson's disease
EP2535416A1 (en) 2011-05-24 2012-12-19 BASF Plant Science Company GmbH Development of phytophthora resistant potato with increased yield
BR102012019434B1 (pt) 2011-07-26 2021-11-09 Dow Agrosciences Llc Métodos de controle de pestes, de insetos, molécula e sequência de dna diagnóstica para o evento de soja 9582.814.19.1
CA3186126A1 (en) 2011-09-21 2013-03-28 Sangamo Biosciences, Inc. Methods and compositions for regulation of transgene expression
EP2612918A1 (en) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
CN104427863B (zh) 2012-01-23 2019-06-28 陶氏益农公司 除草剂耐受性棉花事件pDAB4468.19.10.3
CN107119060A (zh) 2012-02-01 2017-09-01 陶氏益农公司 草甘膦抗性植物和相关方法
US20150225734A1 (en) 2012-06-19 2015-08-13 Regents Of The University Of Minnesota Gene targeting in plants using dna viruses
US20140075593A1 (en) * 2012-09-07 2014-03-13 Dow Agrosciences Llc Fluorescence activated cell sorting (facs) enrichment to generate plants
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
UA119135C2 (uk) * 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
CA2884162C (en) 2012-09-07 2020-12-29 Dow Agrosciences Llc Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
WO2014165612A2 (en) 2013-04-05 2014-10-09 Dow Agrosciences Llc Methods and compositions for integration of an exogenous sequence within the genome of plants
WO2014199358A1 (en) 2013-06-14 2014-12-18 Cellectis Methods for non-transgenic genome editing in plants
US10767188B2 (en) 2013-09-25 2020-09-08 Nutech Ventures Methods and compositions for obtaining useful plant traits
WO2015066638A2 (en) 2013-11-04 2015-05-07 Dow Agrosciences Llc Optimal maize loci
BR102014027442B1 (pt) 2013-11-04 2022-09-27 Dow Agrosciences Llc Molécula de ácido nucleico recombinante, uso de uma planta de milho, parte de planta de milho ou célula de planta de milho compreendendo a mesma e método para produzir uma célula vegetal transgênica compreendendo um dna de interesse
EP3862434A1 (en) 2013-11-04 2021-08-11 Dow AgroSciences LLC Optimal soybean loci
AU2014341928B2 (en) * 2013-11-04 2017-11-30 Corteva Agriscience Llc A universal donor system for gene targeting
AU2014368982B2 (en) 2013-12-19 2021-03-25 Amyris, Inc. Methods for genomic integration
US10683513B2 (en) 2013-12-31 2020-06-16 Dow Agrosciences Llc Tissue-specific expression and hybrid plant production
BR112017000925B1 (pt) 2014-07-14 2023-10-10 Washington State University Método de reprodução de porcos, método para gerar um porco sem nenhuma célula espermatogônica funcional, método de produção de um macho receptor e método de produção de porcos
WO2016098078A2 (en) 2014-12-19 2016-06-23 Novartis Ag Dimerization switches and uses thereof
US20160281055A1 (en) 2015-03-20 2016-09-29 Dow Agrosciences Llc Incorporation of phosphatidylcholine in a media composition
KR20170141217A (ko) * 2015-05-12 2017-12-22 상가모 테라퓨틱스, 인코포레이티드 유전자 발현의 뉴클레아제-매개된 조절
BR112018002417A2 (pt) 2015-08-06 2018-09-18 Univ Missouri animal não humano ou descendente do mesmo ou uma célula de animal, animal suíno, descendente ou célula, célula, descendente, método de reprodução, população de animais, método para aumentar uma resistência de animal, molécula de ácido nucleico, ácido nucleico e ácido nucleico isolado
EP4089166A1 (en) 2016-01-27 2022-11-16 Oncorus, Inc. Oncolytic viral vectors and uses thereof
EP3417061B1 (en) 2016-02-18 2022-10-26 The Regents of the University of California Methods and compositions for gene editing in stem cells
CN109414414A (zh) 2016-03-16 2019-03-01 戴维·格拉德斯通研究所 用于治疗肥胖症和/或糖尿病以及用于鉴定候选治疗剂的方法和组合物
US11293033B2 (en) 2016-05-18 2022-04-05 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
US11078481B1 (en) 2016-08-03 2021-08-03 KSQ Therapeutics, Inc. Methods for screening for cancer targets
US10975393B2 (en) * 2016-08-24 2021-04-13 Sangamo Therapeutics, Inc. Engineered target specific nucleases
SG11201901531TA (en) 2016-08-24 2019-03-28 Sangamo Therapeutics Inc Regulation of gene expression using engineered nucleases
US11078483B1 (en) 2016-09-02 2021-08-03 KSQ Therapeutics, Inc. Methods for measuring and improving CRISPR reagent function
JP7317706B2 (ja) 2016-12-14 2023-07-31 リガンダル インコーポレイテッド 核酸およびタンパク質ペイロード送達のための方法および組成物
KR102720855B1 (ko) 2017-06-15 2024-10-25 더 리전트 오브 더 유니버시티 오브 캘리포니아 표적화된 비-바이러스 dna 삽입
KR20200042904A (ko) 2017-07-26 2020-04-24 온코루스, 인크. 종양용해성 바이러스 벡터 및 그의 용도
KR102503130B1 (ko) 2017-10-27 2023-02-24 더 리전트 오브 더 유니버시티 오브 캘리포니아 내인성 t 세포 수용체의 표적화된 대체
CA3084572A1 (en) 2017-12-18 2019-06-27 Syngenta Participations Ag Targeted insertion sites in the maize genome
CN113226333A (zh) 2018-10-02 2021-08-06 桑格摩生物治疗股份有限公司 用于调控Tau蛋白的方法和组合物
EP3861130A4 (en) * 2018-10-02 2022-08-03 Sangamo Therapeutics, Inc. GENETIC MODULATORS
US20200157531A1 (en) * 2018-11-16 2020-05-21 Wisconsin Alumni Research Foundation Method to enhance screening for homologous recombination in genome edited cells using recombination-activated fluorescent donor delivery vector
US12460230B2 (en) 2019-02-10 2025-11-04 The J. David Gladstone Institutes Modified mitochondrion and methods of use thereof
JP7708737B2 (ja) 2019-07-18 2025-07-15 ユニバーシティー オブ ロチェスター 細胞の細胞型選択的免疫保護
WO2021028359A1 (en) 2019-08-09 2021-02-18 Sangamo Therapeutics France Controlled expression of chimeric antigen receptors in t cells
CN111462823B (zh) * 2020-04-08 2022-07-12 西安交通大学 一种基于dna测序数据的同源重组缺陷判定方法
EP4243609A1 (en) 2020-11-16 2023-09-20 Pig Improvement Company UK Limited Influenza a-resistant animals having edited anp32 genes
WO2022173531A1 (en) 2021-02-10 2022-08-18 Oncorus, Inc. Compounds, compositions, and methods of using thereof
CA3232968A1 (en) 2021-10-14 2023-04-20 Jasper Williams Immune cells having co-expressed shrnas and logic gate systems
WO2023081756A1 (en) 2021-11-03 2023-05-11 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Precise genome editing using retrons
WO2023105244A1 (en) 2021-12-10 2023-06-15 Pig Improvement Company Uk Limited Editing tmprss2/4 for disease resistance in livestock
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024013514A2 (en) 2022-07-15 2024-01-18 Pig Improvement Company Uk Limited Gene edited livestock animals having coronavirus resistance
WO2024044723A1 (en) 2022-08-25 2024-02-29 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024206821A1 (en) 2023-03-31 2024-10-03 Briacell Therapeutics Corp. Methods for enhancing the immunogenicity of cellular vaccines
WO2025049959A2 (en) 2023-09-01 2025-03-06 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0222493A1 (en) 1985-10-04 1987-05-20 Lubrizol Genetics Inc. TR-based sub-TI plasmids
US5422251A (en) 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
DE68918494T2 (de) 1988-05-17 1995-03-23 Lubrizol Genetics Inc Pflanzliches Ubiquitinpromotorsystem.
US5176996A (en) 1988-12-20 1993-01-05 Baylor College Of Medicine Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
FR2646438B1 (fr) 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
US5420032A (en) 1991-12-23 1995-05-30 Universitge Laval Homing endonuclease which originates from chlamydomonas eugametos and recognizes and cleaves a 15, 17 or 19 degenerate double stranded nucleotide sequence
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5792632A (en) 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
DE69334225D1 (de) 1992-07-07 2008-07-31 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US6242568B1 (en) 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
AU704601B2 (en) 1994-01-18 1999-04-29 Scripps Research Institute, The Zinc finger protein derivatives and methods therefor
US5585245A (en) 1994-04-22 1996-12-17 California Institute Of Technology Ubiquitin-based split protein sensor
EP0781331B1 (en) 1994-08-20 2008-09-03 Gendaq Limited Improvements in or relating to binding proteins for recognition of dna
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
US5789538A (en) 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5925523A (en) 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof
GB2338237B (en) 1997-02-18 2001-02-28 Actinova Ltd In vitro peptide or protein expression library
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
BR9810248A (pt) 1997-06-12 2000-09-19 Dow Agrosciences Llc Sequências reguladoras para plantas transgênicas.
ATE401410T1 (de) * 1997-11-18 2008-08-15 Pioneer Hi Bred Int Zusammensetzungen und verfahren für die genetische modifizierung von pflanzen
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AR014491A1 (es) 1998-01-29 2001-02-28 Dow Agrosciences Llc Metodo para obtener plantas transgenicas fertiles de gossypium hirsutum.
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
WO1999045132A1 (en) 1998-03-02 1999-09-10 Massachusetts Institute Of Technology Poly zinc finger proteins with improved linkers
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7030215B2 (en) 1999-03-24 2006-04-18 Sangamo Biosciences, Inc. Position dependent recognition of GNN nucleotide triplets by zinc fingers
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
AU776576B2 (en) 1999-12-06 2004-09-16 Sangamo Biosciences, Inc. Methods of using randomized libraries of zinc finger proteins for the identification of gene function
ATE355368T1 (de) 2000-01-24 2006-03-15 Gendaq Ltd Nucleinsäure bindende polypeptide gekennzeichnet durch flexible linker verbundene nucleinsäuredomäne
US20020061512A1 (en) 2000-02-18 2002-05-23 Kim Jin-Soo Zinc finger domains and methods of identifying same
AU5391401A (en) 2000-04-28 2001-11-12 Sangamo Biosciences Inc Targeted modification of chromatin structure
WO2001088197A2 (en) 2000-05-16 2001-11-22 Massachusetts Institute Of Technology Methods and compositions for interaction trap assays
JP2002060786A (ja) 2000-08-23 2002-02-26 Kao Corp 硬質表面用殺菌防汚剤
CA2435394C (en) 2001-01-22 2018-01-09 Sangamo Biosciences, Inc. Modified zinc finger binding proteins
GB0108491D0 (en) 2001-04-04 2001-05-23 Gendaq Ltd Engineering zinc fingers
AU2002250512A1 (en) 2001-04-05 2002-10-21 The Regents Of The University Of California Robotic device for locomotor training
JP2005500061A (ja) 2001-08-20 2005-01-06 ザ スクリップス リサーチ インスティテュート Cnnについての亜鉛フィンガー結合ドメイン
US7462758B2 (en) 2001-12-20 2008-12-09 Sungene Gmbh & Co. Kgaa Methods for the transformation of vegetal plastids
US7262054B2 (en) 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
WO2003087341A2 (en) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
US20030232410A1 (en) 2002-03-21 2003-12-18 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
JP2006502748A (ja) 2002-09-05 2006-01-26 カリフォルニア インスティテュート オブ テクノロジー 遺伝子ターゲッティングを誘発するキメラヌクレアーゼの使用方法
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
EP3222715A1 (en) 2003-08-08 2017-09-27 Sangamo BioSciences, Inc. Methods and compositions for targeted cleavage and recombination
US20050102713A1 (en) 2003-11-07 2005-05-12 Merlo Donald J. Use of untranslated region of osmotin gene to enhance transgene expression in plants
ATE546536T1 (de) 2004-03-17 2012-03-15 Basf Plant Science Gmbh Verbessertes konstrukt für marker ausschneiden gestützt auf einen doppelfunktion selektionsmarker
CA2578140A1 (en) 2004-09-23 2006-03-30 Basf Plant Science Gmbh Recombination cassettes and methods for sequence excision in plants
CA2603177C (en) 2005-04-04 2016-01-26 Bayer Bioscience N.V. Methods and means for removal of a selected dna sequence
WO2007014275A2 (en) 2005-07-26 2007-02-01 Sangamo Biosciences, Inc. Targeted integration and expression of exogenous nucleic acid sequences

Also Published As

Publication number Publication date
AR108684A2 (es) 2018-09-19
AU2007284748B2 (en) 2013-05-16
TWI424061B (zh) 2014-01-21
PL2049663T3 (pl) 2015-08-31
CA2660485A1 (en) 2008-02-21
JP6652952B2 (ja) 2020-02-26
US20100257638A1 (en) 2010-10-07
JP2016195606A (ja) 2016-11-24
JP2018029603A (ja) 2018-03-01
AR062336A1 (es) 2008-10-29
IL196942A0 (en) 2011-08-01
BRPI0716427A2 (pt) 2014-03-11
SI2049663T1 (sl) 2015-12-31
CN101528924A (zh) 2009-09-09
HK1134320A1 (zh) 2010-04-23
AU2007284748A1 (en) 2008-02-21
EP2395081A1 (en) 2011-12-14
WO2008021207A2 (en) 2008-02-21
EP2049663B1 (en) 2015-02-25
UA106193C2 (ru) 2014-08-11
JP6442442B2 (ja) 2018-12-19
JP2014193179A (ja) 2014-10-09
ES2532002T3 (es) 2015-03-23
US9428756B2 (en) 2016-08-30
US20160326537A1 (en) 2016-11-10
JP5964884B2 (ja) 2016-08-03
JP2010500029A (ja) 2010-01-07
KR101439568B1 (ko) 2014-09-12
DK2049663T3 (en) 2015-06-01
EP2049663A1 (en) 2009-04-22
TW200815593A (en) 2008-04-01
AR105435A2 (es) 2017-10-04
HK1221251A1 (zh) 2017-05-26
CN105296527B (zh) 2020-11-27
KR20090054986A (ko) 2009-06-01
US10669551B2 (en) 2020-06-02
CA2660485C (en) 2016-04-12
CN105296527A (zh) 2016-02-03
CN101528924B (zh) 2015-11-25
IL196942A (en) 2014-06-30

Similar Documents

Publication Publication Date Title
ZA200900787B (en) Zinc finger nuclease-mediated homologous recombination
GB2441851B (en) Energy harvesting devices
TWI366296B (en) Eliminating incorrect battery installation
GB0714246D0 (en) adaptive scheduling
EP1999542A4 (en) ADAPTIVE ENERGY MANAGEMENT
EP2035693A4 (en) WAVE
AU313071S (en) Battery
IL207475A0 (en) Energy harvesting
GB0720484D0 (en) Cells
PL2388527T5 (pl) Sztuczny kominek
ZA201006347B (en) Photovoltaic assembly
EP2196087A4 (en) LURE
IL198699A0 (en) Biogel
ZA200804435B (en) Picking arrangement
IL217463A0 (en) Photovoltaic devices including zinc
EP2169741A4 (en) BATTERY ASSEMBLY
EP2476664A4 (en) AGGREGATE OF ZINC
GB0922606D0 (en) Electric pruner
EP2212973A4 (en) ELECTRICAL CONTACTS COMPATIBLE WITH MICROTCA TECHNOLOGY
GB2459100B (en) Trimmer
GB0607279D0 (en) Electrolytic cells
GB0711416D0 (en) Lead level
GB0722342D0 (en) Bait
GB0607237D0 (en) Electric fires
GB0805281D0 (en) Homologous recombination