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WO2025237946A1 - Amélioration biologique de biomasse bactérienne par élimination de métaux dérivée de chélation métallique - Google Patents

Amélioration biologique de biomasse bactérienne par élimination de métaux dérivée de chélation métallique

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Publication number
WO2025237946A1
WO2025237946A1 PCT/EP2025/062983 EP2025062983W WO2025237946A1 WO 2025237946 A1 WO2025237946 A1 WO 2025237946A1 EP 2025062983 W EP2025062983 W EP 2025062983W WO 2025237946 A1 WO2025237946 A1 WO 2025237946A1
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WO
WIPO (PCT)
Prior art keywords
biomass
fraction
preferably below
bacterial
chelating agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2025/062983
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English (en)
Inventor
Tiago PINTO
Jens DYNESEN
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Unibio Unibio AS
Original Assignee
Unibio Unibio AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unibio Unibio AS filed Critical Unibio Unibio AS
Publication of WO2025237946A1 publication Critical patent/WO2025237946A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/008—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/195—Proteins from microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/20—Proteins from microorganisms or unicellular algae

Definitions

  • the present invention relates to processes for providing a biomass fraction having reduced levels of impurities, from a bacterial biomass. Further, the present invention relates to a bacterial biomass fraction having a low metal ion content.
  • Methylococcus capsulatus is a non-commensal bacterium found ubiquitously in nature. It metabolizes methane, e.g., from natural gas, into biomass, CO 2 and water. Being rich in protein, M. capsulatus can be used as a protein supplement in animal feed and is also of interest for human consumption. The fermentation of this bacterium as a protein source for both animal and human consumption may contribute to satisfying the world's need for dietary protein in a way which is more environmentally friendly than conventional protein production industries.
  • single cell protein (SCP) biomass can be limited in application (e.g. fish feed, dog feed, human feed) due to legal thresholds for chemical impurities present in the final product.
  • chemical impurities e.g. copper salts are known to be extremely toxic to sheep.
  • the presence of such impurities can, in most cases, be address via different process modifications, sometimes even during the fermentation step.
  • chemical impurities in the form of certain metal ions e.g. copper and iron
  • Methylococcus capsulatus specifically in its membrane-bound form of particulate methane monooxygenase, responsible for methane uptake and thus carbon source intake for growth, and cannot be simply removed under process instability consequences.
  • chelating agents - such as EDTA - in bacterial biomass must be limited, as they would in most cases beconsidered as processing aids, and as such, would have a maximum allowed concentration (currently around 0.1 % w/w). It is therefore an object of embodiments of the invention to provide a bacterial biomass with reduced chemical impurities in the form of certain metal ions, e.g. copper and iron, as well as processes for the production thereof.
  • the resulting product is less harmful, in that it comprises reduced metal ion concentrations.
  • a process for providing a biomass fraction from a bacterial biomass, said biomass fraction having a reduced metal ion content relative to said bacterial biomass, said process comprising the steps of: a. contacting the bacterial biomass with a chelating agent to form a biomass mixture, and allowing metal ions in said biomass mixture to become bound to said chelating agent, b. subjecting said biomass mixture to a separation step, to provide: a biomass fraction having a reduced metal ion content compared to the bacterial biomass, and metal-bound chelating agent.
  • a combined bacterial fermentation and purification process comprising the steps of: i. fermenting a bacterial culture comprising Methylococcus capsulatus in the presence of a gaseous carbon source and a fermentation substrate, to provide a bacterial biomass,
  • bacterial biomass fractions are also provided, according to claims 21-28.
  • a human or animal feed comprising or consisting of the bacterial biomass fraction is also provided.
  • Fig. 1 shows an overview of one process according to the invention.
  • Fig. 2 shows an overview of another process according to the invention.
  • FIG. 3 shows an overview of a further process according to the invention.
  • Fig. 4 shows an overview of a further process according to the invention.
  • biomass refers to a proteinaceous product, which may be in the form of a protein extract and comprises cell wall materials of single celled microorganisms from pure or mixed cultures of algae, yeasts, fungi, or bacteria.
  • biomass fraction refers to a fraction, i.e., a part of the biomass.
  • single cell protein commonly refers to a proteinaceous product isolated from single celled microorganisms.
  • the proteinaceous product may be in the form of a biomass or a protein extract and comprises cell wall materials of single celled microorganisms from pure or mixed cultures of algae, yeasts, fungi, or bacteria.
  • the single cell protein is traditionally used as an ingredient or a substitute for protein-rich foods and is suitable for human consumption or as animal feeds.
  • Utilizing microorganisms to obtain biomass for use in feed and food results in a product that has a higher proportion of nucleic acids than conventional foods.
  • concentration of nucleic acids present in SCP varies depending on the specific microorganism employed, generally about 5 to 18 percent nucleic acids (dry weight) are present in SCP.
  • the abbreviation "DM" refers to "Dry Matter”.
  • dry matter and “ash” content is determined according to the A.O.A.C. method (reference A.O.A.C. Standard, 1945).
  • dry weight in the context of the dry weight of an M. capsulatus biomass, should be taken to mean the weight of the biomass after all water has been removed from it. This should not be taken to mean that all water has been removed in all embodiments of an M. capsulatus biomass according to the present invention, since water is present in some embodiments. It should rather be understood as a measure that can be used to reproducibly calculate whether a certain biomass falls within the scope of the biomass according to the present invention. All w/w % provided are on a dry matter basis.
  • chelating agent refers to a molecule, which is able to bond metal ions. Chelation typically involves the formation of two or more separate coordinate bonds between a polydentate ligand and a single metal ion.
  • the chelating agent may be e.g. covalently bound to a polymer matrix.
  • the invention provides a process for providing a biomass fraction from a bacterial biomass, said biomass fraction having a reduced metal ion content relative to said bacterial biomass.
  • the process comprises a first step of (a) contacting the bacterial biomass with a chelating agent to form a biomass mixture, and allowing metal ions in said biomass mixture to become bound to said chelating agent.
  • the contacting step - step a - typically takes place in aqueous suspension, where the biomass and the chelating agent are suspended.
  • Step a. may take place under one or more of the following conditions: a pH between 5 and 11, preferably between 6 and 7, a temperature between 5 and 90 °C, preferably between 10 and 50 °C, more preferably between 20 and 40 °C, a time of between 0.5 minutes and 60 minutes, preferably between 1 minute and 30 minutes.
  • the process is a flow process, in which bacterial biomass flows across immobilised chelating agent.
  • the process is a chromatography process in which the bacterial biomass is passed through a chromatography column containing immobilised chelating agent.
  • the chelating agent is immobilised on a solid phase.
  • the solid phase may comprise a material selected from the group consisting of glass, a polymer (e.g. a polymer hydrogel or polymer resin), silica, metal (e.g. metal-organic frameworks), and combinations thereof, preferably wherein the chelating agent is immobilised on a polymer.
  • the chelating agent may be in solution phase.
  • a complex comprising metal ions are bound to the chelating agent precipitates from the solution, and can be separated.
  • the chelating agent is suitably a metal ion chelating agent, preferably EDTA, Ethylenediamine, Glycine, Citric acid, Gluconic acid, Tartaric acid, Hexametaphosphoric acid, Pyrophosphoric acid, Tripolyphosphoric acid, Phytic acid, free histidine, L-glutamic acid, N,N- diacetic acid, Aspartic acid, citrate, gluconate, or salts thereof, particularly sodium salts thereof, e.g. sodium citrate (TSA), sodium gluconate; preferably sodium EDTA, EDTA, sodium citrate (TSA), sodium gluconate, or mixtures thereof.
  • the chelating agent is preferably EDTA or sodium EDTA chemically bonded to a metal-organic framework or to a polymer, e.g. in the form of polymer beads.
  • Immobilisation of the chelating agent may take place by means of chemical bonding of the chelating agent to the solid phase.
  • Chemical bonding between the chelating agent and the solid phase may be for instance be covalent, ionic or via hydrogen bonds.
  • Immobilised chelating agents are commercially available from known chemical suppliers.
  • the process comprises a second step (b) of subjecting said biomass mixture to a separation step, to provide: biomass fraction having a reduced metal ion content compared to the bacterial biomass, and metal-ion bound chelating agent.
  • immobilised chelating agent to which metal ions are bound, is separated from the mixture, to leave a biomass fraction having a reduced metal ion content. Separation can take place via e.g. filtration, centrifugation, or simply passing the biomass over the immobilised chelating agent in a supported bed, e.g. a chromatography column.
  • the process may further comprise the step of subjecting said bacterial biomass to an initial separation step to remove a first liquid fraction prior to step a.
  • the bacterial biomass may be suspended in a liquid, e.g. water, or diluted with e.g. water prior to step a.
  • the process may also further comprise one or more purification steps performed on the biomass fraction, after step b, said purification steps selected from washing and centrifugation. It is preferred that steps a. and b. take place sequentially, i.e. without intervening steps.
  • the process further comprises a step of regenerating the chelating agent and a metal-ion rich solution from said metal ion-bound chelating agent after step b.
  • Regeneration of the chelating agent can take place by washing the metal ion-bound chelating agent with e.g. an aqueous acidic solution, such as an a sulfuric acid solution, so as to separate the metal ion from the chelating agent.
  • an aqueous acidic solution such as an a sulfuric acid solution
  • the metal-ion rich solution can be used as a component of the fermentation substrate.
  • the metal ions of interest may be selected from copper, iron, cobalt, manganese, calcium, molybdenum, zinc and magnesium ions.
  • the process is particularly beneficial, when the metal ions are selected from copper and iron ions.
  • the concentration of copper ions in the biomass fraction achieved in the present process may be less than 300 mg/kg biomass, preferably less than 200 mg/kg, more preferably 20-150 mg/kg, most preferably around 25 mg/kg.
  • the concentration of iron ions in the biomass fraction achieved in the present process may be less than 1000 mg/kg biomass, preferably less than 500 mg/kg, preferably less than 300 mg/kg, preferably below 250 mg/kg.
  • the concentration of zinc ions in the biomass fraction may be less than 300 mg/kg biomass, preferably less than 200 mg/kg, more preferably 20-150 mg/kg, most preferably around 100 mg/kg.
  • the concentration of cobalt ions in the biomass fraction may be less than 25 mg/kg biomass, preferably less than 10 mg/kg, more preferably 0.1-1.0 mg/kg, most preferably around 0.3 mg/kg.
  • the concentration of manganese ions in the biomass fraction may be less than 50 mg/kg biomass, preferably less than 15 mg/kg, more preferably 2-10 mg/kg.
  • the concentration of magnesium ions in the biomass fraction may be less than 10 mg/kg biomass, preferably less than 2 mg/kg, more preferably 0.1-1 mg/kg.
  • the concentrations of copper, iron, cobalt, manganese, zinc and magnesium ions in the biomass fraction are - in combination - as specified above.
  • a combined bacterial fermentation and purification process comprising the steps of: i. fermenting a bacterial culture comprising Methylococcus capsulatus in the presence of a gaseous carbon source and a fermentation substrate, to provide a bacterial biomass,
  • the fermentation step comprises at least one metal ion selected from copper, iron, cobalt, manganese, calcium, molybdenum, zinc and magnesium ions.
  • the concentration of copper ions in the fermentation step (i) is at least 200 mg/kg biomass, e.g. 200-400 mg/kg biomass, and wherein the concentration of iron ions in the fermentation step (i) is at least 400 mg/kg biomass, e.g. 400-600 mg/kg biomass.
  • the optimal metal ion concentration in the fermentation step can be achieved, while the (lower) optimal metal ion concentration in the biomass fraction can also be achieved.
  • the biomass material (which is typically an aqueous biomass material) is suitably obtained from the fermentation of at least one microorganism, preferably wherein at least one of the microorganisms is a bacterial cell, preferably a methanotroph, more preferably M. capsulatus.
  • the microorganism In a fermentation step, the microorganism, or mixture of microorganisms, metabolizes methane into aqueous biomass material, CO 2 , and water.
  • the fermentation occurs in fermentation tanks, and the process is described in detail in, e.g., WO 2017/080987 and WO 2022/008478 hereby incorporated by reference.
  • the biomass material is a single-cell protein (SCP) product. It comprises mainly of protein (ca. 60%), and lesser amounts of RIMA and DNA.
  • SCP single-cell protein
  • the biomass material is an aqueous suspension. In this aqueous suspension, the majority of the solid component is cellular material from the microorganism. Other components (e.g., proteins, nucleic acids, polysaccharides, lipids, LPS or other small molecules) may be dissolved or suspended in the aqueous phase.
  • At least one of the microorganisms used in the fermentation step is suitably a bacterial cell, preferably a Gram-negative bacterial cell, preferably a methanotroph, more preferably M. capsulatus. Therefore, the biomass is suitably M. capsulatus biomass.
  • Method "Methylococcus capsulatus” or "M. capsulatus”, as used herein, can mean any strain of bacteria belonging to the M. capsulatus species.
  • the strain may be either naturally occurring or developed in a laboratory, such as a genetically modified strain.
  • naturally occurring means that the strain has not been genetically modified using genetic engineering techniques. However, it may contain natural modifications or alterations in its genetic material compared to a reference strain, such as alterations that occur randomly during replication.
  • the strain is naturally occurring.
  • the strain is M. capsulatus (Bath), more preferably the M. capsulatus (Bath) identified under NCIMB 11132.
  • the methanotrophic bacteria may be provided in a co-fermentation together with one or more heterotrophic bacteria.
  • the following heterotrophic bacteria may be particularly useful to co-ferment with M. capsulatus; Ralstonia sp. ; Bacillus brevis; Brevibacillus agri; Alcaligenes acidovorans; Aneurinibacillus danicus and Bacillus firmus.
  • Suitable yeasts may be selected from species of Saccharomyces and/or Candida.
  • the preferred heterotrophic bacteria are chosen from Alcaligenes acidovorans (NCIMB 13287), Aneurinibacillus danicus (NCIMB 13288) and Bacillus firmus (NCIMB 13289) and combinations thereof.
  • the methanotrophic bacteria and/or the heterotrophic bacteria may be genetically modified.
  • the carbon source is converted by the microorganism(s) to biomass material.
  • the carbon source comprises methane, and is e.g., natural gas, syngas, or biogas.
  • the carbon source is dissolved in the fermentation medium. Fermentation suitably takes place in a U-loop reactor, as described in WO 2010/069313, hereby incorporated by reference.
  • a suitable fermentation medium is described in e.g., WO 2018/158322 hereby incorporated by reference.
  • the fermentation step has a relatively low Dry Matter content, e.g., below 5%.
  • the co-fermentation of M. capsulatus with one or more other organisms may result in a biomass product containing an M. capsulatus biomass as well as a biomass of the one or more other organisms.
  • the M. capsulatus is fermented in combination with one or more bacteria selected from: Ralstonia sp., B. brevis, B. agri, A. acidovorans, A. danicus, and B. firmus; preferably any one or two or all three of: A. acidovorans, A. danicus, and B. firmus; more preferably any one or two or all three of: A. acidovorans (NCIMB 13287), A. danicus (NCIMB 13288), and B. firmus (NCIMB 13289). Further details of the fermentation process are described in WO 2020/245197 and WO 2020/249670, which are hereby incorporated by reference.
  • the dry matter content of the biomass material from the fermentation process is between 1- 3%.
  • the biomass material may be clarified, and the supernatant from the clarification step may be recycled to the fermenter. Pellets are thus obtained with a dry matter content of 10-18%.
  • the fermentation broth in the fermenter may preferably continuously be provided with the required amounts of water and nutrient salts, such as ammonium/ammonia, magnesium, calcium, potassium, iron, copper, zinc, manganese, nickel, cobalt and molybdenum in the form of sulphates, chlorides or nitrates, phosphates and pH controlling components, i.e. acids and/or bases, as normally used by the skilled person, e.g.
  • sulphuric acid H 2 SO 4
  • nitric acid HNO 3
  • sodium hydroxide NaOH
  • potassium nitrate KNO 3
  • the latter is also a suitable nitrogen source for M. capsulatus.
  • suitable substrates etc. are described in WO 2000/70014 and WO 2010/069313, which are incorporated by reference.
  • the biomass material produced from fermentation of natural gas will typically comprise from 60 to 80% by weight crude protein; from 5 to 20% by weight crude fat; from 3 to 12% by weight ash; from 3 to 15% by weight nucleic acids (RIMA and DNA).
  • a bacterial biomass fraction is thus provided, having a copper ion content below 5% w/w, more preferably below 2% w/w, more preferably below 1% w/w, more preferably below 0.5% w/w, more preferably below 0.2% w/w, more preferably below 0.1% w/w of said biomass fraction. Furthermore, a bacterial biomass fraction is provided having an iron ion content below 5% w/w, more preferably below 2% w/w, more preferably below 1% w/w, more preferably below 0.5% w/w, more preferably below 0.2% w/w, more preferably below 0.1% w/w of said biomass fraction.
  • the biomass fraction has a copper ion content below 5% w/w, more preferably below 2% w/w, more preferably below 1% w/w, more preferably below 0.5% w/w, more preferably below 0.2% w/w, more preferably below 0.1% w/w of said biomass fraction and an iron ion content below 5% w/w, more preferably below 2% w/w, more preferably below 1% w/w, more preferably below 0.5% w/w, more preferably below 0.2% w/w, more preferably below 0.1% w/w of said biomass fraction.
  • the bacterial biomass fraction has a copper ion content below 0.1% w/w of said biomass fraction and an iron ion content below 0.1% w/w of said biomass fraction.
  • the bacterial biomass fraction may alternatively or additionally have one or more of the following : a zinc ion content below 0.1% w/w, more preferably below 0.025% w/w, more preferably below 0.015% w/w, more preferably below 0.01% w/w of said biomass fraction; a magnesium ion content below 0.01% w/w, more preferably below 0.001% w/w, more preferably below 0.0001% w/w of said biomass fraction; a cobalt ion content below 0.01% w/w, more preferably below 0.0025% w/w, more preferably below 0.0001% w/w, more preferably below 0.00003% w/w of said biomass fraction; a manganese ion content below 0.005% w/w, more preferably below 0.0005% w/w, more preferably below 0.0002% w/w, more preferably below 0.0001% w/w of said biomass fraction.
  • a zinc ion content below 0.1% w/w, more
  • biomass fraction is obtained from the fermentation of at least one microorganism, preferably wherein at least one of the microorganisms is a bacterial cell, preferably a methanotroph, more preferably M. capsulatus.
  • An animal feed or human food product may comprise or consist of the bacterial biomass fraction described herein. Such a product has an improved toxicity profile. It can be directly fed to animals or provided as a food product (e.g. as ingredient or final product) for humans.
  • Figure 1 shows a process in which biomass from fermentation is contacted with chelating agent.
  • a separation step e.g. centrifucation
  • the biomass from fermentation may first be centrifuged.
  • the supernatant is removed, before contacting the remaining biomass with chelating agent.
  • Figure 2 shows a two-step process in which biomass from fermentation is first centrifuged. The supernatant is removed, and the remaining biomass is then subjected to a chromatography step in which it is contacted with immobilised chelating agent to provide a biomass fraction having reduced metal ion content.
  • Figure 3 shows a multiple-step process, in which biomass from fermentation is first centrifuged, as per the process of Figure 2. The supernatant is removed, and the resulting biomass is washed and then centrifuged again. The second biomass is subjected to a chromatography step in which it is contacted with immobilised chelating agent to provide a biomass fraction having reduced metal ion content.
  • Figure 4 shows a process for the regeneration of the immobilised chelating agent e.g. by washing the immobilised chelating agent with an aqueous acidic solution, such as a sulfuric acid solution, so as to provide a metal-rich solution ion and a chelating agent. Subsequently, the metal-ion rich solution can be used as a component of the fermentation substrate.
  • an aqueous acidic solution such as a sulfuric acid solution
  • a bacterial biomass (as obtained by the processes exemplified in e.g. WO 2017/080987 and WO 2022/008478) is subjected to a first centrifugation (using an SPX centrifuge running at 9,000 rpm and discharge time set to 120 seconds, and fed with 500 L per hour and delivering 50-60 L of precipitate per hour and 440-450 L of supernatant per hour) to provide a first bacterial biomass fraction enriched in dry matter (first precipitate, 10-15 % dry matter), and a first supernatant low in dry matter (1-2 % dry matter).
  • a first centrifugation using an SPX centrifuge running at 9,000 rpm and discharge time set to 120 seconds, and fed with 500 L per hour and delivering 50-60 L of precipitate per hour and 440-450 L of supernatant per hour
  • the first bacterial biomass fraction is eluted through a chromatography column containing immobilized EDTA in metal-organic framework surface.
  • the resulting eluted biomass fraction content of copper and iron amounts to 40-50 % relative to the first bacterial biomass concentration on a dry matter basis by quantification of metal ions using inductively coupled plasma mass spectrometry.
  • a bacterial biomass (as obtained by the processes exemplified in e.g. WO 2017/080987 and WO 2022/008478, having 10-15 % dry matter) is contacted with a chelating agent, said agent being EDTA, to a final concentration of approximately 10 mM, at a temperature of 90 °C. Said mixture was then centrifuged (using a centrifuge running at 5,000 rpm and 90 °C) and the resulting biomass fraction was compared to the bacterial biomass for metal ion concentrations (table below). The resulting biomass fraction had significant lower concentration of metal ions.

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Abstract

L'invention concerne des procédés pour fournir une fraction de biomasse à partir d'une biomasse bactérienne, présentant une teneur réduite en ions métalliques par rapport à ladite biomasse bactérienne. L'invention concerne également une fraction de biomasse bactérienne présentant une faible teneur en ions métalliques particuliers. L'invention concerne également un aliment pour animaux comprenant ou constitué de la fraction de biomasse bactérienne.
PCT/EP2025/062983 2024-05-15 2025-05-13 Amélioration biologique de biomasse bactérienne par élimination de métaux dérivée de chélation métallique Pending WO2025237946A1 (fr)

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WO2001060974A2 (fr) 2000-02-16 2001-08-23 Norferm Da Procede
WO2010069313A2 (fr) 2008-12-15 2010-06-24 Ebbe Busch Larsen Fermenteur en forme de u et/ou à buse à boucle en u et procédé de fermentation
WO2017080987A2 (fr) 2015-11-09 2017-05-18 Unibio A/S Procédé permettant une meilleure fermentation d'un micro-organisme
WO2018158322A1 (fr) 2017-03-01 2018-09-07 Unibio A/S Nouveau milieu de fermentation pour la croissance de bactéries méthanotrophes et procédé de production dudit milieu
WO2020245197A1 (fr) 2019-06-07 2020-12-10 Unibio A/S Procédé d'optimisation d'un processus de fermentation
WO2020249670A1 (fr) 2019-06-13 2020-12-17 Unibio A/S Procédé de régulation d'un processus de fermentation
WO2021071895A1 (fr) * 2019-10-07 2021-04-15 Calysta, Inc. Compositions alimentaires comprenant un isolat de protéine de methylococcus capsulatus
WO2022008478A2 (fr) 2020-07-07 2022-01-13 Unibio A/S Processus de production de protéine unicellulaire

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Publication number Priority date Publication date Assignee Title
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