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WO2025228993A1 - Filtre pour culture cellulaire et plateforme de culture cellulaire associée - Google Patents

Filtre pour culture cellulaire et plateforme de culture cellulaire associée

Info

Publication number
WO2025228993A1
WO2025228993A1 PCT/EP2025/061725 EP2025061725W WO2025228993A1 WO 2025228993 A1 WO2025228993 A1 WO 2025228993A1 EP 2025061725 W EP2025061725 W EP 2025061725W WO 2025228993 A1 WO2025228993 A1 WO 2025228993A1
Authority
WO
WIPO (PCT)
Prior art keywords
cell culture
filter
mesh
ring
well
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2025/061725
Other languages
English (en)
Inventor
Virgile Nicolas Robert VIASNOFF
Zeynep KARATAS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Cnrs@create Ltd
National University of Singapore
Original Assignee
Centre National de la Recherche Scientifique CNRS
Cnrs@create Ltd
National University of Singapore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Cnrs@create Ltd, National University of Singapore filed Critical Centre National de la Recherche Scientifique CNRS
Publication of WO2025228993A1 publication Critical patent/WO2025228993A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/26—Constructional details, e.g. recesses, hinges flexible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes

Definitions

  • the present invention relates, in general terms, to a filter and a cell culture platform comprising the filter thereof.
  • Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. After cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator. These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or rich medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and gases (CO2, 02), and regulates the physio-chemical environment (pH buffer, osmotic pressure, temperature). Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar.
  • a liquid, semi-solid, or solid growth medium such as broth or agar.
  • culture cells Among the common manipulations carried out on culture cells are media changes, passaging cells, and transfecting cells. These are generally performed using culture methods that rely on aseptic technique. Aseptic technique aims to avoid contamination with bacteria, yeast, or other cell lines. Manipulations are typically carried out in a biosafety cabinet or laminar flow cabinet to exclude contaminating micro-organisms.
  • the present disclosure provides a filter for use in a cell culture well, comprising: a) a ring sized to fit within the cell culture well; and b) a mesh attached to a planar side of the ring.
  • the ring is sized such that its diameter is about 0.1 mm to about 20 mm smaller than a diameter of the cell culture well.
  • the ring is configured to frictionally engage an inner wall of a cell culture well.
  • the ring is formed from an elastic material. In some embodiments, the ring is characterised by an inner diameter of about 1 mm to about 30 mm.
  • the ring is characterised by an outer diameter of about 2 mm to about 35 mm.
  • the ring is characterised by a wall thickness of about 1 mm to about 20 mm.
  • the ring is characterised by a height of about 2 mm to about 15 mm.
  • the ring comprises a recess or hole on at least one of its planar side.
  • the mesh is formed from Nylon.
  • the mesh is a rectangular mesh, wherein the mesh is characterised by a mesh size of about 20 pm by about 20 pm to about 900 pm by about 900 pm.
  • mesh is characterised by a mesh size of about 20 mesh/inch to about 500 mesh/inch.
  • the filter is positioned within the cell culture well such that it is substantially perpendicular to a longitudinal axis of the cell culture well.
  • the filter is positioned within the cell culture well such that it is in contact with a bottom of the cell culture well.
  • the filter is positioned within the cell culture well such that the mesh is adjacent to a bottom of the cell culture well.
  • the present disclosure also concerns a cell culture platform, comprising: a) at least one cell culture well; b) a filter as disclosed herein positioned within the at least one cell culture well, the filter partitions the at least one cell culture well into a first compartment and a second compartment. wherein the at least one filter is configured to allow fluid communication of a cell culture medium in both directions between both compartments and cell movement only from the first compartment to the second compartment.
  • the cell culture platform further comprises a second filter, the second filter positioned on top of the filter and within the cell culture well.
  • the second filter comprises a mesh with a larger mesh size than the filter.
  • the first filter and the second filter are spaced apart by at least about 1 mm.
  • FIG. 1 Schematic drawing of the filters ring inside a well with cavities during seeding phase (A) and spheroid (B). Filters can be use together or separately, d: distance between the two filters 0.1>d>3cm
  • FIG. 2 Example of the filter of the invention A and different filter rings size for different culture vessel as I Bl DI and 24 Aggrewell plate.
  • Figure 3 Representative imaging without (A) and with (B) filter on the top of Aggrewells.
  • Figure 4 A: Organoids in aggrewell plate with Blocking Device before and after 1 medium exchange step (100% of the wells remained filled).
  • Figure 5 An embodiment of the filter of the present disclosure.
  • Figure 6 An image of the filter in a cell culture well when in use.
  • Figure 7 Two photos (A - B) of another embodiment of the filter of the present disclosure.
  • the present disclosure is predicated on the understanding that spheroids/organoids are cultured in different types of cell culture vessels. However, there can be some loss of organoids during their formation and during various manipulation steps (e.g. drug treatment, washing, etc).
  • the present disclosure concerns a filter for use with a 3D cell culture well.
  • the filter according to the invention can also be called blocking device.
  • the filter may be sized to allow cells to pass through but not organoids. This allows a user to obtain homogeneous and stable spheroids/organoids throughout the various steps of the experiment up to imaging.
  • the filter disperses the cells that are seeded in the wells and lead to an homogeneous seeding of the cell number in each of the microwells. It enhances the reproducibility and the standardization of the initial cell number in each well.
  • the organoids may be cultured, treated (fixed) and imaged (immunostained) in the same culture well throughout the experiment. This makes it possible to follow the organoid through to imaging.
  • the present disclosure concerns a filter for use in a cell culture well, comprising: a) a ring sized to fit within the cell culture well; and b) a mesh attached to a planar side of the ring.
  • the mesh is attached to the ring such that it covers the ring hole.
  • the size or diameter of the ring may be adjusted according to the size of the cell culture well.
  • the size of the mesh may be adjusted according to the type of cells being cultured.
  • the mesh can also be called filter cloth.
  • the ring breaks the flow of medium at each manipulation by reducing the shear force and lifting force. Thus, the medium is easily changed without any loss of organoids.
  • the ring is sized to fit within the cell culture well.
  • the ring is sized such that its diameter is slightly small than the diameter of the cell culture well.
  • the ring may have a diameter which is about 0.1 mm to about 20 mm smaller than the diameter of the cell culture well.
  • the ring is sized to be adjacent to an inner wall of the cell culture well.
  • the ring is sized such that it is spaced apart from the inner wall by about 0.5 mm to about 20 mm.
  • the ring is configured to engage an inner wall of a cell culture well. In some embodiments, the ring is configured to frictionally engage an inner wall of a cell culture well.
  • the ring may be formed from an elastic material.
  • the ring may be formed from an elastic polymer.
  • the elastic polymer may be a silicon, such as polydimethylsilane (PDMS).
  • the elastic polymer may be rubber.
  • the ring may be made from polyoxymetylene (POM). Such materials are resistance to wear and tear, and also has a high resistance to chemicals and hydrolysis.
  • the ring is sized to fit into a cell culture well.
  • the ring may be sized to fit in a well of a 6-well plate, 12-well plate, 24-well plate, 48-well plate, 96-well plate, 384- well plate, 1536-well plate and ibidi p-Dish 35 mm.
  • the ring is characterised by an inner diameter of about 1 cm to about 30 cm.
  • the ring may have an inner diameter of about 1 mm to about 28 mm when used with a 6-well plate, about 1 mm to about 14 mm when used with a 12- well plate, about 1 mm to about 12 mm when used with a 24-well plate, about 1 mm to about 2 mm when used with a 96-well plate, or about 1 mm to about 25 mm when used with a 35 mm p-Dish.
  • the ring is characterised by an outer diameter of about 2 mm to about 35 mm. In some embodiments, the ring is characterised by an outer diameter of about 1 .55 cm.
  • the ring may have an outer diameter of about 31 mm to about 33 mm when used with a 6-well plate, about 17 mm to about 18 mm when used with a 12- well plate, about 15 mm to about 16 mm when used with a 24-well plate, about 3 mm to about 4 mm when used with a 96-well plate, or about 27 mm to about 28 mm when used with a 35 mm p-Dish.
  • the ring is characterised by a wall thickness.
  • the wall thickness is the distance between the inner diameter and the outer diameter.
  • the wall thickness may be about 1 mm to about 20 mm. It was found that when the wall thickness is in this range, the filter is sturdy enough to be inserted within the cell culture well without deformation and tearing of the mesh.
  • the ring may have a wall thickness of about 1 mm to about 15 mm when used with a 6-well plate, about 1 mm to about 9 mm when used with a 12-well plate, about 1 mm to about 7 mm when used with a 24-well plate, about 1 mm to about 2 mm when used with a 96-well plate, or about 1 mm to about 13 mm when used with a 35 mm p-Dish.
  • the ring is characterised by a height of about 2 mm to about 15 mm.
  • the height of the ring corresponds to the depth of the well. The height thus allows the mesh to be spaced apart from the bottom of the well.
  • the ring is characterised by a height of about 3 mm. It was found that when the height is in this range, the filter is sturdy enough to be inserted within the cell culture well without deformation and tearing of the mesh. Further, the mesh may be spaced such that the filter does not disturb or affect the growth of the organoid.
  • the ring may have a height of about 2 mm to about 11 mm when used with a 6-well plate, about 2 mm to about 12 mm when used with a 12-well plate, about 2 mm to about 10 mm when used with a 24-well plate, about 2 mm to about 6 mm when used with a 96-well plate, or about 2 mm to about 5 mm when used with a 35 mm p-Dish.
  • the ring comprises a groove or hole on at least one of its planar side.
  • the groove may extend partway or at least halfway through the height of the ring.
  • the hole may be a through-hole, which runs substantially perpendicular to the ring's planar side.
  • the groove or hole allows a user to insert or remove the filter into the cell culture well by using tweezers (Figure 7).
  • the ring comprises a planar side (or first planar side). The other planar side is thus the second planar side.
  • the mesh is attached to the first planar side of the ring as shown in Figure 5.
  • the attachment may be via the wall thickness.
  • the attachment may be by an adhesive, such as NOA (Norland Optical Adhesive 73) or epoxy. In particular, the adhesive may be biocompatible.
  • the mesh substantially overlaps with the ring across its wall thickness. It was found that this allows the mesh to be securely fixed to the ring and provides a homogenous surface when contacted with the bottom of the well.
  • the mesh size may be configured based on the type of cells being cultured and if organoids are being formed. For example, stem cells have a size of about 10 pm, thus a mesh size of about 50 pm by about 100 pm may be used. After 1 st day of culture, the organoids that are formed have a size about 50 pm to about 60 pm, and the 6 th day the size reaches around 200 pm. Thus, the mesh size of about 50 pm by about 100 pm is sufficient to maintain the organoids within the cell culture well.
  • the mesh is a screen mesh. In some embodiments, the mesh is a rectangular mesh. In other embodiments, the mesh is formed from a woven fabric or a nonwoven fabric.
  • the fabric may be Nylon, or in particular Nylon 66.
  • mesh is characterised by a mesh size of about 20 pm by about 20 pm to about 900 pm by about 900 pm.
  • the mesh size may be chosen according to the expected size of the organoids. For example, for organoids that will be about 80 pm after 24 hours of culture, a mesh size of 50 pm x 100 pm may be used, or a mesh size of 50 pm x 50 pm may be used.
  • mesh is characterised by a mesh size of about 20 mesh/inch to about 500 mesh/inch.
  • the mesh size is about 40 mesh/inch to about 500 mesh/inch, about 60 mesh/inch to about 500 mesh/inch, about 80 mesh/inch to about 500 mesh/inch, about 100 mesh/inch to about 500 mesh/inch, about 120 mesh/inch to about 500 mesh/inch, about 140 mesh/inch to about 500 mesh/inch, about 160 mesh/inch to about 500 mesh/inch, about 180 mesh/inch to about 500 mesh/inch, about 200 mesh/inch to about 500 mesh/inch, about 250 mesh/inch to about 500 mesh/inch, about 300 mesh/inch to about 500 mesh/inch, about 350 mesh/inch to about 500 mesh/inch, or about 400 mesh/inch to about 500 mesh/inch.
  • mesh is characterised by a mesh size of about 20 pm to about 900 pm.
  • the filter comprises a second mesh attached to the second planar side of the ring.
  • the mesh may have the same mesh size, or may have a different mesh size relative to the first mesh. The two meshes are thus separated by a distance provided by the ring height.
  • the filter thus sits within the cell culture well as the ring is sized to be slightly smaller than the diameter of the inner wall.
  • the filter is configured to frictional ly engage an inner wall of a cell culture well via the ring.
  • the filter is positioned within the cell culture well such that it is substantially perpendicular to the longitudinal axis of the well.
  • the filter may be positioned at a bottom of the well such that the second planar side of the ring is in contact with the bottom of the well.
  • the mesh is thus spaced apart from the bottom of the well, where the cells may adhere.
  • the cell culture well may comprise cavities, or microwells at the bottom of the well.
  • the filter may be positioned at the bottom of the well such that the first planar side of the ring (and hence the mesh) is adjacent to the bottom of the well.
  • the first planar side (and hence the mesh) is in close proximity with the bottom of the well.
  • the first planar side (and hence the mesh) is in contact with the bottom of the well ( Figure 6).
  • the second planar side is thus facing the opening of the well.
  • a substantial portion of the cell medium may be removed and fresh cell medium added back to fill the well.
  • 2 ml of medium may be used for the cell/organoids culture.
  • Half of the medium may be removed by pipetting 1 ml above the filter and 1 ml of new media is added.
  • the filter breaks the (turbulent or laminar) flow of the medium as it is removed or added back in, thus at least reduce the force acting on the cells or organoids. This helps maintain their viability.
  • the filter also prevents the cells or organoids from being removed together with the medium.
  • the filter may also be used with adherent cell lines. Some adherent cells may be loosely attached to the cell culture vessel. In such cases, the filter may be used to prevent the flow and the loss of cells during medium exchange.
  • the present disclosure also concerns a cell culture platform, comprising: a) at least one cell culture well; b) a filter as disclosed herein positioned within the at least one cell culture well, the filter partitions the at least one cell culture well into a first compartment and a second compartment. wherein the at least one filter is configured to allow fluid communication of a cell culture medium in both directions between both compartments and cell movement only from the first compartment to the second compartment.
  • cell medium may be exchanged.
  • the cell culture platform further comprises a second filter, the second filter positioned on top of the first filter and within the cell culture well.
  • the first filter may be a trap filter, to prevent the loss of organoids when cell medium is changed.
  • the second filter may be homogeneity filter, to allow a homogenous dispersion of cell seeding. The space between these two filters allows the cells to be dispersed more homogenously over the whole surface.
  • the second filter acts to further reduce the forces acting on the cells and/or organoids. It allows for homogeneous cell seeding and homogeneous organoid size across the wells, and allows for keeping of organoids/spheroids inside the well from the beginning till the end of the cell culture.
  • the second filter may have a different mesh size, or a same mesh size as the first filter.
  • the first filter may have a mesh size of about 50 pm by about 100 pm.
  • the second filter may have a mesh size of about 100 pm by about 100 pm.
  • the first filter and the second filter are spaced apart by at least about 1 mm. In other embodiments, the spacing is at least about 2 mm, about 3 mm, about 4 mm, about 5 mm, about 6 mm, about 7 mm, about 8 mm, or about 10 mm.
  • the second filter is configured as the first filter.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un filtre destiné à être utilisé dans un puits de culture cellulaire, comportant : un anneau dimensionné pour s'adapter à l'intérieur du puits de culture cellulaire ; et un maillage fixé à un côté plan de l'anneau. La présente invention concerne également une plateforme de culture cellulaire, comportant au moins un puits de culture cellulaire ; un filtre selon l'invention, lequel, placé à l'intérieur dudit au moins un puits de culture cellulaire, divise ledit au moins un puits de culture cellulaire en un premier compartiment et un deuxième compartiment ; ledit au moins un filtre étant conçu pour permettre la communication fluidique d'un milieu de culture cellulaire dans les deux sens entre les deux compartiments et le mouvement des cellules uniquement du premier compartiment vers le deuxième compartiment. L'invention concerne le domaine de la culture cellulaire.
PCT/EP2025/061725 2024-04-29 2025-04-29 Filtre pour culture cellulaire et plateforme de culture cellulaire associée Pending WO2025228993A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG10202401233P 2024-04-29
SG10202401233P 2024-04-29

Publications (1)

Publication Number Publication Date
WO2025228993A1 true WO2025228993A1 (fr) 2025-11-06

Family

ID=95696857

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2025/061725 Pending WO2025228993A1 (fr) 2024-04-29 2025-04-29 Filtre pour culture cellulaire et plateforme de culture cellulaire associée

Country Status (1)

Country Link
WO (1) WO2025228993A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5958762A (en) * 1995-06-15 1999-09-28 Chemodyne S.A. Cell culture device
US20080076170A1 (en) * 2006-09-27 2008-03-27 Tuija Annala Cell culture insert and cell culture vessel
EP2230014A1 (fr) * 2009-03-20 2010-09-22 Mark Ungrin Dispositifs et procédés pour la production d'agrégats cellulaires
US20210139833A1 (en) * 2019-11-07 2021-05-13 Rutgers, The State University Of New Jersey Multilayer stackable tissue culture platform for 3d co-culture

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5958762A (en) * 1995-06-15 1999-09-28 Chemodyne S.A. Cell culture device
US20080076170A1 (en) * 2006-09-27 2008-03-27 Tuija Annala Cell culture insert and cell culture vessel
EP2230014A1 (fr) * 2009-03-20 2010-09-22 Mark Ungrin Dispositifs et procédés pour la production d'agrégats cellulaires
US20210139833A1 (en) * 2019-11-07 2021-05-13 Rutgers, The State University Of New Jersey Multilayer stackable tissue culture platform for 3d co-culture

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FUTREGA KATHRYN ET AL: "Themicrowell-mesh: A novel device and protocol for the high throughput manufacturing of cartilage microtissues", BIOMATERIALS, ELSEVIER, AMSTERDAM, NL, vol. 62, 20 May 2015 (2015-05-20), pages 1 - 12, XP029222882, ISSN: 0142-9612, DOI: 10.1016/J.BIOMATERIALS.2015.05.013 *

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WO2025228993A1 (fr) Filtre pour culture cellulaire et plateforme de culture cellulaire associée