WO2025226844A1

WO2025226844A1 - Aquatic environment analysis system and related methods, apparatuses, and software

Info

Publication number: WO2025226844A1
Application number: PCT/US2025/026019
Authority: WO
Inventors: Bryan D. KLEBON; Aaron Akiva RUBIN; Alexander E. TAYLOR; Kenny Mani
Original assignee: Lamotte Chemical Products Co
Current assignee: Lamotte Chemical Products Co
Priority date: 2024-04-23
Filing date: 2025-04-23
Publication date: 2025-10-30
Anticipated expiration: 2026-10-23

Abstract

Systems and methods for an aquatic environment for measuring and/or otherwise determining constituents of an aquatic environment and calibrating such measurements and/or determinations along with related components, such as a holder and holder cover combination with specific fluid flow characteristics. Calibration of one or more chemical indicators using a calibration solution in contact with the one or more chemical indicators as well as calibration of aquatic environment fluid in between an optical sensor and a chemical indicator may also be part of a system and/or method.

Description

AQUATIC ENVIRONMENT ANALYSIS SYSTEM AND RELATED METHODS, APPARATUSES, AND SOFTWARE

RELATED APPLICATION DATA

[0001] This application claims the benefit of priority of U.S. Provisional Patent Application Serial No. 63/637,864, filed on April 23, 2024, and titled “Aquatic Environment Analysis System and Related Methods, Apparatuses, and Software,” which is incorporated by reference herein in its entirety.

DETAILED DESCRIPTION

[0002] Various systems, methods, apparatuses, and software (along with related features, components, concepts, and the like) are discussed herein that relate in part to monitoring and/or analyzing an aspect of an aquatic environment. An “aquatic environment” is any environment wherein water is present. In one exemplary aspect, it may be desired to determine a value for a quality of a constituent of the water in an aquatic environment. Examples of a quality include, but are not limited to, presence, absence, amount, concentration, and any combinations thereof of one or more constituents (e.g., a chemical, a mineral, an organic matter, an inorganic matter, a bacteria, a conductive material, an ionic material, a metabolite, a gas, a molecular organism, etc.) of the water of the aquatic environment. Determining a value for a quality of a constituent may be referred to herein as determining a level of the constituent. Additionally, a quality of a constituent may include a derivative of a measured value, such as a conductivity measurement, a color of a fluid or other material of an aquatic environment, a density of a fluid or other material of an aquatic environment, a turbidity of a fluid of an aquatic environment, a clarity of a fluid of an aquatic environment, a pH measurement, another property of an aqueous environment, another property of water, etc. In one example, a constituent includes a dissolved material. In another example, a constituent includes an undissolved material.

[0003] Examples of an aquatic environment include, but are not limited to, an aquarium, a sump of an aquarium, a plumbing component of an aquarium, a swimming pool, a diving pool, a wave pool, a hot tub, a fish pond, a potable water supply (e.g., a household water system, a commercial office water system, etc.), a non-potable water supply (e.g., a household non-potable water system), a sewage treatment infrastructure, a water treatment system, a water fountain, a water display, a lake, a lagoon, a food processing system, an aquaculture environment, a recirculating aquaculture system, a submerged oceanic aquaculture system, a river aquaculture system, an estuary aquaculture system, a fish transport system, an invertebrate transport system, an animal transport system, a supporting equipment component of any of the foregoing, and any combinations thereof. Examples of a supporting equipment component include, but are not limited to, a plumbing component, a heater, a filter, a skimmer, a control system, a holding tank, a display tank, a filtration canister, and any combinations thereof. Examples of a plumbing component system include, but are not limited to, a sump, a pump, a pipe, a storage chamber, a valve, a refugium, a quarantine chamber, and any combinations thereof.

[0004] While the examples listed above may include physical structures (e.g., sump, pump, pipe, etc.), the relevant portion of the “aquatic environment” is the water-related component within or related to the physical structure. For example, when reference is made herein to being in, calibrating, measuring, analyzing, or other reference to an aquatic environment, such a reference includes doing such an action or relationship with respect to a fluid (i.e., water containing fluid) of the relevant aquatic environment.

[0005] A system, device, apparatus, component, or other related aspect of an embodiment of the current disclosure, or any subportion thereof, may be located in (e.g., placed in, submersed in, etc.) a fluid of an aquatic environment. In one example, a system, device, apparatus, component, or other related aspect (or any subportion thereof) may be located in line with a fluid flow of an aquatic environment (e.g., in line with fluid flow in a main body of water, in line with fluid flow within a supporting equipment component, etc.). Additionally, it is contemplated that one or more portions of a system, device, apparatus, component, or other related aspect (or any subportion thereof) may be made a part of, or associated with, a structure of supporting equipment component.

[0006] A level of a constituent or other characteristic of a fluid or other material of an aquatic environment may be determined by an aquatic environment analysis system of the current disclosure by any number of any suitable mechanism or technology for determining a level of the constituent or other characteristic of a fluid or other material. Examples of such a mechanism or technology include, but are not limited to, a chemical indicator, an electrode, a chemical probe, an ion selective electrode, an optical probe, a gravimetric detector, a refractometer, and any combinations thereof. A chemical indicator is a mechanism and/or material that undergoes a physical change or possesses some other physically detectible characteristic related to a level of a constituent. In one aspect, such a physical characteristic can be sensed and/or read (e.g., using an optical reader) to provide a value related to the level of the constituent. A chemical indicator may include an indicator that is reactive to and/or otherwise capable of undergoing a physical change related to any type of constituent or subcomponent of a constituent for which a value is desired. Examples of a constituent include, but are not limited to, pH, hardness, calcium, magnesium, oxygen, carbon dioxide, ammonia, phosphate, nitrate, potassium, nitrite, carbon, a molecular organism, a metabolite, a mineral, an inorganic matter, an organic matter, a living organism (e.g., a bacteria, virus, metabolite of an organism, etc.), a conductive material, a heavy meal, a pathogen, and any combinations thereof. A constituent may be in any form in an aquatic environment including, but not limited to, a solid, a dissolved matter, an undissolved matter, an ion, a gas, and any combinations thereof.

[0007] A chemical indicator may have any known form for adequately exposing a chemical indicator to an aquatic environment and determining a physical change thereof related to a level of a desired constituent. Example forms for a chemical indicator include, but are not limited to, a powder, a dye, a liquid, an immobilized material, a strip, a patch, and any combinations thereof. A chemical indicator may include components in addition to a reactive and/or otherwise physically changing component. Example components for a chemical indicator include, but are not limited to, a chemical indicator material, an immobilizing medium, an optical filtering film, an optical blocking film, and any combinations thereof. A chemical indicator may include a dye and/or other material that is reactive or otherwise capable of having a physical and/or chemical change or other detectible characteristic with respect to a desired constituent (e.g., a physical constituent in the water and/or other property of the water). In one example, a chemical indicator is a dye. In another example, a chemical indicator is an immobilized dye. Example immobilizing mediums include, but are not limited to, a gel, a hydrogel, a solgel, an aerogel, a chalcogel, a polymer matrix, a cellulosic matrix, and any combinations thereof. In one such example, a chemical indicator includes a dye covalently bonded to a cellulose fiber that is immobilized in a hydrogel. Example dyes include, but are not limited to, a calcium detecting aminonaphthalimide, a calcium detecting perylenediamide, a magnesium detecting dye based on a aminonaphthalimide, a magnesium detecting dye based on a photon induced electron transfer process (PET), a magnesium detecting dye based on a intramolecular charge transfer process (ICT), a magnesium detecting perylenediamide, a carbon dioxide sensitive dye based on a aminonaphthalimide, a carbon dioxide sensitive dye based on a photon induced electron transfer process (PET), a carbon dioxide sensitive dye based on a intramolecular charge transfer process (ICT), a carbon dioxide sensitive perylenediamide, and any combinations thereof. [0008] A chemical indicator may include a chemical indicator material that is reactive and/or capable of a physical and/or chemical change or other detectible characteristic related to a desired constituent based on any of a variety of processes. Examples of such a process include, but are not limited to, fluorescence, fluorescence decay, phase fluorescence, electromagnetic energy absorptance, electromagnetic energy absorbance, change in electromagnetic energy absorptance, change in electromagnetic energy absorbance, electromagnetic energy reflectivity, change in electromagnetic energy reflectivity, color, change in color, change in refractive index, refractive index, conductivity, change in conductivity, and any combinations thereof. In one example, a chemical indicator includes a chemical indicator material that is fluorescent. In another example, a chemical indicator includes a chemical indicator material that is colorimetric.

[0009] A chemical indicator may be a reversible chemical indicator. A chemical indicator (e g., a chemical indicator that is submersed or otherwise put into contact with a fluid of an aquatic environment) may include a chemical indicator material that is stable in water (e.g., one including a dye that remains in an immobilized medium such that it does not mix with and/or change the water in which it is in contact). Such stability may be assisted with the use of other components of a chemical indicator (e.g., a film applied to the indicator to resist exposure to water (e.g., from a side of an indicator that is not to be reactive), an immobilizing medium, etc.).

[0010] In one exemplary implementation, an aquatic environment analysis system includes a monitoring/measuring apparatus and a chemical indicator holder having one or more chemical indicators. A monitoring/measuring apparatus (e.g., an optical sensing portion) is an apparatus having one or more optical readers that are designed and configured to detect a physical characteristic (e.g., a physical change) of a chemical indicator. An optical reader includes components for detecting a physical characteristic of a chemical indicator such as, for example, one or more optical lenses, one or more light pipes, one or more sensors capable of detecting light and/or other energy from a chemical indicator, a light source (e.g., an LED) capable of producing a light and/or other energy for delivery to a chemical indicator (e.g., an excitation energy for causing a chemical indicator to emit an emission energy /light, such as via fluorescence, under certain circumstances related to a constituent in water), etc. In one example, an optical reader is configured to detect a light/energy from a chemical indicator that results from a characteristic of the chemical indicator (e.g., a wavelength of light from the chemical indicator due to ambient light, such as by reflectance of light not absorbed by the chemical indicator). In another example, an optical reader is configured to produce a light/energy to deliver to a chemical indicator and to detect light/energy emitted from the chemical indicator (e.g., via fluorescence). In still another example, an optical reader is configured to detect light/energy emitted from a chemical indicator (e.g., fluorescence from another excitation energy source, luminescence, etc.). Combinations of the previous examples are contemplated.

[0011] Various optical readers are disclosed with additional details in U.S. Patent No. 9,023,281 to Clark, entitled “Submersible Chemical Indicator Apparatuses for Use in Aquatic-Environment Monitoring/Measuring Systems” (the “’281 Patent”) the disclosure of which related to optical readers and their details is incorporated herein by reference. Other variations of optical readers will be understood by those of ordinary skill.

[0012] A monitoring/measuring apparatus may include one or more optical readers. In one example where a monitoring/measuring apparatus includes a plurality of optical readers, the optical readers are aligned such that each optical reader can detect from and/or provide light/energy to a corresponding chemical indicator of a chemical indicator holder that is positioned proximate to the monitoring/measuring apparatus. In another example where a monitoring/measuring apparatus includes a plurality of optical readers, the optical readers are configured for different optical purposes (e.g., one optical reader for emissive type detection, such as providing excitation energy /light and detecting emitted light, and another optical reader for colorimetric detection). In yet another example, where a monitoring/measuring apparatus includes a plurality of optical readers, a corresponding holder having one or more chemical indicators thereon can be moved with respect to the monitoring/measuring apparatus and/or the monitoring/measuring apparatus can be moved with respect to the holder in order to align one or more of the chemical indicators at different times with different optical readers. Various mechanisms and approaches for moving a chemical indicator holder and/or monitoring/measuring apparatus with respect to each other are disclosed in the ‘281 Patent, the disclosure of which is incorporated herein by reference. Additional variations of such movement will be evident to a person of ordinary skill in light of the same and the current disclosure. Further, such relative movement may also be a feature of a monitoring/measuring apparatus having only a single optical reader. For example, a monitoring/measuring apparatus with one optical reader can be positioned proximate a chemical indicator holder with a plurality of chemical indicators and the holder and/or monitoring/measuring apparatus can be moved with respect to each other (e.g., with a motor and/or other mechanisms of the monitoring/measuring apparatus) in order to align the optical reader with different chemical indicators at different times. Movement in either a one optical reader or plurality optical reader system can also be for purposes of aligning an optical reader with one of a plurality of regions on a single chemical indicator apparatus (e.g., in order to read from a different portion of the chemical indicator apparatus, such as to reduce light degradation, to take multiple readings of a same constituent, etc.).

[0013] A chemical indicator holder (also referred to herein as a holder) is an apparatus for supporting one or more chemical indicators to be read by an optical reader of a corresponding monitoring/measuring apparatus. In one example, one or more chemical indicators are attached to a holder on a side that is configured to be positioned opposite of a side facing an optical reader of a monitoring/measuring apparatus. In such an example, an optical reader may read from a chemical indicator on the holder by one or more of a variety of mechanisms. Examples of such mechanisms include having a window in the holder, having the holder be made of an optically transparent material, and any combinations thereof. A window for a chemical indicator holder may take a variety of forms that will allow the optical reader to detect light/energy from the chemical indicator and/or deliver light/energy to the chemical indicator (e.g., each type of light/energy being of a desired wavelength or wavelength range for the particular detection of physical change in the particular chemical indicator). Examples of such mechanisms include, but are not limited to, having a section of the chemical indicator holder include an opening through which an optical reader can optically see the chemical indicator, having a section of the chemical indicator be made of a material that is optically transparent material, and any combinations thereof. An optically transparent material (e.g., for a window, for a holder material, for an adhesive) may be selected and configured based on a variety of considerations. Example considerations for selecting and configuring an optically transparent material for a chemical indicator holder and/or window and/or adhesive include, but are not limited to, matching the optical transparency to one or more wavelengths of light/energy that can be detected from a chemical indicator (e.g., via reflectance, via emission, etc.), matching the optical transparency to one or more wavelengths of light/energy necessary for an excitation energy /light of a chemical indicator, ensuring surface consistency in a material to minimize light/energy scattering and/or physical interference with light/energy transmission through the material to the chemical indicator, ensuring the material does not fluoresce or otherwise emit light/energy at a wavelength that is to be detected by a corresponding optical reader (e.g., a wavelength that will interfere with light/energy from a chemical indicator), ensuring the material is not excited to fluoresce or otherwise emit light/energy by a wavelength of light ambient to the holder and/or provided by a corresponding optical reader, and any combinations thereof. It is noted that in any particular example, optical transparency may not be perfect transparency and may be a partial transparency (e.g., a partial transparency sufficient for desired readings from a chemical indicator).

[0014] A chemical indicator may be positioned on, in, or otherwise associated with a chemical indicator holder in a variety of ways that will be understood in light of the current disclosure. Example ways include, but are not limited to, using an adhesive to adhere a side of a chemical indicator (e.g., an immobilized dye indicator) to a surface of a holder; using an adhesive to edge mount a chemical indicator (e.g., an immobilized dye indicator) to a surface of a holder; at least partially enclosing a chemical indicator (e.g., a powdered indicator in solution) in a chamber, repository, or other structural enclosure of a holder; connecting a chemical indicator (e.g., via a snap connector, a screw connector, a pressure connector, etc.); printing (e.g., with a deposition printing device) a chemical indicator on a surface of a holder; spraying a chemical indicator on a surface of a holder; and any combinations thereof. Examples of adhesives that can be used include, but are not limited to, a cyanoacrylate adhesive, silicone adhesive, double-sided adhesive, spray adhesive, epoxy, contact cement, pva glue, polyurethane glue, polyvinyl acetate, and any combinations thereof. Considerations for selection of a specific adhesive include, but are not limited to, optical transparency at a wavelength of energy desired to be read by an optical reader (e.g., light energy emitted from a chemical indicator, light energy reflected from a chemical indicator, each such as in presence or absence of a target constituent of an aquatic environment); optical transparency at a wavelength of energy of an excitation energy used to excite a chemical indicator (e.g., for fluorescence or other emissive physical characteristics in presence or absence of a target constituent of an aquatic environment); lack of fluorescence or other emissive characteristic of a material of the adhesive caused by a wavelength of light that would interfere with a reading of an optical reader (e.g., is the same as or close to a light energy produced by an optical reader or an ambient light that may impinge on a corresponding chemical indicator holder); lack of fluorescence or other emissive characteristic of a material of the adhesive at a wavelength of light that would interfere with a reading of an optical reader (e.g., is the same as or close to a light energy to be read by an optical reader); and any combinations thereof.

[0015] A chemical indicator holder may be constructed of any suitable material for the particular system (e.g., that has proper transparency when needed, is structurally sufficient to hold a desired one or more chemical indicators, is stable in water when applicable for contact with water, does not fluoresce or otherwise emit in an interfering manner, is structurally sufficient to withstand movement relative to a monitoring/measuring apparatus, and any combinations thereof). Example materials for a chemical indicator holder and/or window of a chemical indicator holder include, but are not limited to, a plastic, a polymer, an acrylic, a cyclic olefin copolymer (“COP”), a quartz, a glass, a polyethylene terephthalate (“PET”), and any combinations thereof. A chemical indicator holder may take any of a variety of forms and/or shapes. Examples of a form for a chemical indicator holder include, but are not limited to, solid, fenestrated, trussed, stretched membrane, and any combinations thereof. Examples of a shape for a chemical indicator holder (or portion thereof) include, but are not limited to, round, planar, discoidal, cylindrical, frusto-conical, spherical, ellipsoidal, parallelepiped, cuboid, and any combinations thereof. Various forms and shapes examples and details for a chemical indicator holder are disclosed in the ‘281 Patent, the disclosure of which is incorporated herein by reference. In one exemplary aspect, a holder may have any shape and/or form that allows for the holder to hold one or more chemical indicators and to have such one or more chemical indicators be read by a corresponding optical reader. In one example, a chemical indicator holder includes at least a portion of the holder having a discoidal shape. Variations of discoidal shaped holders are further described herein (e.g., with respect to FIGS. 11A to 11G and other locations). A chemical indicator holder may be constructed of a monolithic material. A chemical indicator holder may be constructed such that it includes portions made of different materials (e.g., constructed of two or more parts, with parts made of different materials).

[0016] A chemical indicator holder may include one or more reservoirs designed and configured to hold a calibration solution such that the calibration solution contacts one side of one or more chemical indicators. Examples of such reservoirs will be described further below. In one exemplary aspect, a reservoir for holding a calibration solution is positioned such that the calibration solution (when the calibration solution is present) is in contact with a side of a chemical indicator that is opposite a side of the chemical indicator that is to be positioned such that an optical reader can read from that opposite side and/or direct light/energy (e.g., for excitation) to the chemical indicator. A holder may have more than one reservoir and each reservoir may partially enclose more than one chemical indicator. A reservoir of a chemical indicator holder includes one or more openings that individually and/or together are configured to allow a calibration solution that may be located in the reservoir to remain in the reservoir when desired and to remove the calibration solution when desired. In one example, a reservoir includes a selectively openable opening that includes a flow controlling feature that regulates the retention and/or removal of the calibration solution from the reservoir via an opening in the reservoir. Examples of such a feature include, but are not limited to, a door, a cover, a valve, a foil cover, a film cover, a paper cover, an aperture size and/or configuration designed to control fluid flow, a dissolvable membrane, a selectively dissolvable membrane (e.g., a biopolymer), and any combinations thereof. The one or more openings (and/or selectively openable opening feature(s)) may also be configured to allow water/fluid from an aquatic environment to enter the reservoir and come into contact with the one or more chemical indicators at least partially enclosed by the reservoir (e.g., when and/or after the calibration solution has been removed from the reservoir). In one example, a reservoir opening is covered by a removable foil. In another example, a reservoir opening is covered by a removable film. It is contemplated that a reservoir for holding a calibration solution in contact with one or more chemical indicators may be formed by a structure of a cover over the corresponding one or more chemical indicators. In such a case, the reservoir opening may be the absence of that structure once removed (e.g., the space formed by the removal). For example, a chemical indicator on a surface of a holder may be covered by a film or foil cover that adheres to the surface of the holder (e g., forming a bubble over the surface and the chemical indicator) and contain a volume of a calibrations solution under the cover in contact with the chemical indicator. Removal of the cover in such an example (e.g., after taking a calibration reading) would allow the calibration solution to be removed and could remove all or part of any structure forming the reservoir such that a reservoir opening is formed and the one or more chemical indicators that were enclosed are now open to be placed in contact with a fluid of an aquatic environment to be monitored/measured.

[0017] A chemical indicator holder and corresponding optical reader of a monitoring/measuring apparatus may be positioned such that a space exists between the chemical indicator holder and the optical reader. In one such example, the side of a chemical indicator holder that is adjacent the space between the holder and the optical reader is a side that is opposite the location of one or more reservoirs of the holder (i.e., opposite a side of the holder having one or more chemical indicators attached thereto). The space between a holder and an optical reader (and the adjacent portions of the holder and monitoring/measuring apparatus) are designed and configured to have one or more openings to the space that allow for water/fluid of an aquatic environment to which the holder and monitoring/measuring apparatus are placed into contact (e.g., via submersion in or otherwise location in the aquatic environment) to enter into the space. Examples of such design and configuration are described further below.

[0018] An optical reader of a monitoring/measuring apparatus is connected to one or more processing functionalities for communicating any raw measurement data/values to such processing functionality. The processing functionality may be part of the monitoring/measuring apparatus and/or part of a device that is connected to the monitoring/measuring apparatus. Such a connection may include a wired and/or wireless connection. Example wireless and wired connections are discussed further below with respect to network connections for connecting one apparatus to another. In one example, a monitoring/measuring apparatus includes wireless networking circuitry and additional processing and/or connective circuitry that is configured to receive data/values from an optical reader and communicate such data/values to an external computing device. In such an example, the external computing device processes the received data/values to determine a level of a predetermined constituent represented by the data/values. Other examples are contemplated where a portion or all of the processing of raw detected data/values from an optical reader to determine a level of a predetermined constituent represented by the data/values takes place by processing circuitry that is a part of the monitoring/measuring apparatus having the optical reader.

[0019] FIG. 2 illustrates an exemplary network environment 200 for a monitoring/measuring apparatus (or other related component of an aquatic environment analysis system) for connection to one or more remote devices (e.g., for transfer of data related to a calibration reading, measurement of a level of a constituent, other data; for processing of an optical reading; for processing of a calibration; for receiving a control instruction from a user for a component of a monitoring/measuring apparatus, for another disclosed feature or aspect herein, and any combinations thereof). In FIG. 2, a monitoring/measuring apparatus 205 is connected to a network 210. Network 210 may include any one or more networks. A cloud resource 215 is connected to network 210. Cloud resource 215 is a network connected information source and/or data store for monitoring/measuring apparatus 205. Cloud resource 215 may be a resource operated by a manufacturer or seller of monitoring/measuring apparatus 205 to provide monitoring/measuring apparatus 205 with one or more functionalities and/or communication capabilities (e.g., to a user’s device). Cloud resource 215 may also include one or more publicly accessible information sources. A cloud resource may include one or more computing devices, each having one or more memory devices associated therewith having computer readable instructions thereon that include instructions for performing any one or more of the functionalities.

[0020] A user computing device 220 is connected to cloud resource 215 via a network 225.

Network 225 may include one or more of the same networks of network 210. Device 220 may include a display device (e.g., an LCD display device, a touchscreen, etc.). Device 220 may include and/or be associated with machine executable instructions (e.g., stored in a memory) in the form of an app configured to provide a user interface to monitoring/measuring apparatus 205. The app may include any functionalities for operation of an aquatic environment analysis system, related method or apparatus as disclosed herein and/or displaying information (e.g., calibration information, information related to a level of a constituent of water or other results of a monitoring/measuring apparatus analyzing an aspect of an aquatic environment) to and interacting with a user of monitoring/measuring apparatus 205. It is noted that in one or more alternative implementations, a monitoring/measuring apparatus may be configured to communicate directly (e.g., via a network) to a user computing device (e.g., without need for a cloud resource). It is also noted that any processing (e.g., of a calibration reading and/or a measurement of a level of a constituent) may be distributed across one or more devices (e.g., of a monitoring/measuring apparatus, a cloud resource, a user device, etc.). Examples of a network and examples of a computing device are discussed in more detail below.

[0021] An optical reader may be configured to take a calibration reading of a chemical indicator of a chemical indicator holder that is positioned proximate to the optical reader while a calibration solution is in a reservoir of the chemical indicator holder and in contact with the chemical indicator being read for the calibration reading (e.g., prior to removal via one or more openings in the reservoir and prior to allowing water/fluid of an aquatic environment to enter the reservoir and contact the chemical indicator). In one example of such a calibration reading, at least a portion of a chemical indicator holder and corresponding monitoring/measuring apparatus are placed into contact with (e.g., via submersion and/or location in) an aquatic environment such that at least a portion of the aquatic environment fluid/water enters into a space between the holder and an optical reader of the monitoring/measuring apparatus via an opening to the space created by the design and configuration of the holder and monitoring/measuring apparatus. The optical reader is configured (e.g., via circuitry and/or programming) to take a calibration reading from a chemical indicator of the holder that is in contact with a calibration solution in a reservoir of the holder (and not in contact with water/fluid from the corresponding aquatic environment). The calibration reading is taken through the space between the holder and the optical reader and through the fluid/water of the aquatic environment in the space. Further examples of such a calibration reading are discussed further below.

[0022] A secondary calibration reading may also be taken that calibrates to the fluid/water of the aquatic environment located in the space between a holder and an optical reader. Such a reading may be taken at any time during the operation of the corresponding system (e.g., while a calibration solution is located in a reservoir of the holder, after a calibration solution is removed from a reservoir of a holder, after a fluid/water of an aquatic environment is allowed to enter a reservoir of a holder, and combinations thereof).

[0023] A monitoring/measuring apparatus may be configured (e.g., via processing circuitry and related machine executable instructions of the monitoring/measuring apparatus and/or a connected device) to use a calibration reading taken through a fluid/water of an aquatic environment from a chemical indicator (while a calibration solution is in contact with one side of the chemical indicator) and/or a calibration reading taken of a fluid/water of the aquatic environment located in a space between the corresponding holder and optical reader to calibrate a measurement of a characteristic of the chemical indicator measured/detected/otherwise read from the chemical indicator while the chemical indicator is in contact with a fluid/water of the aquatic environment that is in a reservoir of the holder once occupied by the calibration solution.

[0024] FIGS. 1 A and IB illustrate one exemplary embodiment of an aquatic environment analysis system 100. FIG. 1A shows a cross sectional view of system 100. System 100 includes a monitoring/measuring apparatus 105 and a chemical indicator holder 110. Monitoring/measuring apparatus 105 includes an optical reader 115. Holder 110 includes a chemical indicator 120 that is positioned within a reservoir 125 of holder 110. Reservoir 125 includes a surface 130 (e.g., located at the bottom of a reservoir, such as reservoir 125) that is opposite of a surface 135 of holder 110. Chemical indicator 120 has a first side that is positioned proximate (e.g., attached to, located against, etc.) surface 130 and at least a second side that is exposed to the space of reservoir 125 (e.g., such that the second side of indicator 120 can be exposed to a fluid/liquid within reservoir 125, such as a calibration solution and/or a fluid of an aquatic environment to be analyzed).

[0025] FIG. IB shows a top-down view of system 100, looking downward at chemical indicator 120 in reservoir 125 through an opening 140 in reservoir 125. Opening 140 is shaped, sized, and configured to allow a calibration solution that may be included in reservoir 125 (e.g., when holder 110 is provided to a user of system 100, when added to reservoir 110 by a user, when added to reservoir 110 by a pump (e.g., a peristaltic pump, an automated pump, a pressurized pump, other pump), when added to reservoir 110 by a gravity fed flow, when added to reservoir 110 by a syphon, when added to reservoir 110 by a vacuum, and any combinations thereof) to be removed and to allow a fluid of an aquatic environment to be analyzed to enter reservoir 125. An opening, such as opening 140, may include a plurality of separate openings (e.g., one or more for removal of a calibration solution and one or more for allowing a fluid of an aquatic environment to enter). An opening, such as opening 140 may include a flow controlling feature.

[0026] In one example, a holder, such as holder 110, is provided to a user of an aquatic environment monitoring system with a calibration solution in a chemical indicator reservoir, such as reservoir 125. In such an example, one or more openings, such as opening 140, of the reservoir may include a feature (e.g., a cover, a flow restricting aperture, etc.) that allows the calibration solution to remain in the reservoir (e.g., during transit, during positioning with respect to a monitoring/measuring apparatus, during calibration of a chemical indicator therein, during storage in a cartridge, during storage in a reel, and any combinations thereof) and allows the calibration solution to be removed from the reservoir (e.g., via removal of the feature, such as a cover, after calibration with the calibration solution). Removal of a calibration solution may include repositioning of a calibration solution to a different portion (e.g., different chamber) of a chemical indicator holder. After a calibration solution is removed from a reservoir, a fluid of an aquatic environment to be analyzed can be allowed to enter the reservoir (e.g., via the same opening that the calibration solution exited and/or another opening to the reservoir).

[0027] In another example, a holder, such as holder 110, includes a reservoir, such as reservoir 125, and a storage chamber connected to the reservoir via an opening having a feature (e.g., an aperture size, length, surface tension, and/or other configuration; a one direction valve; and any combinations thereof) that allows a calibration solution in the reservoir to move from the reservoir to the storage chamber (e.g., via application of a force, such as shaking and/or rotation of the corresponding holder). In such an example, a reservoir may include an additional opening having a feature (such as a cover and/or one way flow functionality) that can allow a fluid of an aquatic environment to be analyzed to enter the reservoir (e.g., via removal of a cover, opening of a valve, application of a force to have flow occur through a one direction aperture/valve, etc.).

[0028] Chemical indicator holder 110 and monitoring/measuring apparatus 105 are positioned with respect to each other in system 100 such that a space 145 exists between holder 110 and apparatus 105 (i.e., between optical reader 115 and surface 135/chemical indicator 120). Holder 110 and apparatus 105 are designed and configured to have an opening 150 to space 145 that is shaped configured to allow a fluid of an aquatic environment to be analyzed to enter space 145 (e.g., via inline flow, via location of at least a portion of holder 110 and apparatus 105 in the aquatic environment, etc.). The bidirectional arrow shown in FIG. 1 A between optical reader 115 and chemical indicator 120 is for illustration purposes in showing that light/energy from chemical indicator 120 can pass to optical reader 115 (e.g., through space 145 and any fluid from an aquatic environment therein) and that light/energy from optical reader 115 can pass to chemical indicator 120 (e g., through space 145 and any fluid from an aquatic environment therein), each depending on the particular configuration of system 100 and optical reader 115. At least a portion of holder 110 between optical reader 115 and chemical indicator 120 is optically transparent (e.g., includes a window, includes an optically transparent material, etc.).

[0029] In one example, holder 110 is configured such that a fluid of an aquatic environment in space 145 does not contact chemical indicator 120 (e.g., does not contact the side of chemical indicator 120 proximate surface 130) such that chemical indicator 120 cannot be reactive or otherwise undergo a physical change with respect to one or more constituents within the fluid within space 145. In one such example, holder 110 includes a window and chemical indicator 120 includes an at least selectively nonpermeable film preventing contact of a fluid in space 145 with chemical indicator 120 through the window. In another such example, holder 110 includes an optically transparent material between surface 130 and surface 135 between optical reader 115 and chemical indicator 120 that is nonpermeable to a fluid of an aquatic environment in space 145.

[0030] Chemical indicator holder 110 and monitoring/measuring apparatus 105 may be held in position with respect to each other (e.g., such that optical reader 115 is capable of aligning with chemical indicator 120) by any of a variety of ways and/or physical structures. Examples of ways and/or physical structures for having a chemical indicator holder and a monitoring/measuring apparatus be located with respect to each other with a space, such as space 145, therebetween and alignment capability for an optical reader and chemical indicator include, but are not limited to, connecting a chemical indicator holder to a monitoring/measuring apparatus, having a chemical indicator holder and/or optical reader of a monitoring measuring apparatus be part of a structure of an aquatic environment, having a chemical indicator holder and/or optical reader of a monitoring measuring apparatus be part of a supporting equipment component, and any combinations thereof. Example mechanisms for connecting a chemical indicator holder to a monitoring/measuring apparatus include, but art not limited to, a physical connection, a magnetic connection, a removable connection, a connection allowing for relative movement of a holder to a monitoring/measuring apparatus with respect to each other, a snap fit connection, a pressure fit connection, a connection via a connection hub of monitoring/measuring apparatus, and any combinations thereof. In one example, a chemical indicator holder is removably connectible to a monitoring/measuring apparatus. In another example, a chemical indicator holder is magnetically connectible to a monitoring/measuring apparatus. In yet another example, a chemical indicator holder is removably and magnetically connectible to a monitoring/measuring apparatus.

[0031] A chemical indicator holder and a corresponding monitoring/measuring apparatus (and/or optical reader of the same) may be movable with respect to each other in any of a variety of ways known to those of ordinary skill. In one example, a monitoring/measuring apparatus may include a motor for providing movement to a connected chemical indicator holder. Other examples of mechanisms and related details for such movement are disclosed in the ‘281 Patent, the disclosure of which is incorporated herein by reference.

[0032] A monitoring/measuring apparatus may include a connection hub. A connection hub is a physical structure having a size, shape, and configuration for removably connecting a chemical indicator holder. Such a removable connection may include a magnetic connection between the connection hub and the corresponding chemical indicator holder (e.g., the connection hub and the holder each include magnets matched to magnetically connect to each other). In one such example, a connection hub is sized, shaped, and configured to fit with one or more corresponding structures of a chemical indicator holder and also magnetically connect to each other. A connection hub may be connected to a motor (or other movement mechanism) of a monitoring/measuring apparatus for providing movement to the connection hub that can be translated to a connected chemical indicator holder. In one such example, a connection hub is physically connected to a motor (e.g., via an axis) for having the motor drive movement of the connection hub. In another such example, a connection hub is moveably connected to a structure of a monitoring/measuring device (e.g., via a moveable bearing system, etc.) and a motor of the monitoring/measuring device is connected to one or more moveable magnets for driving movement of the moveable magnets. In such a case, the corresponding connecting hub includes magnets that interact with the moveable magnets physically connected to the motor to translate movement from the motor to the connection hub (e.g., that can further translate to movement of a chemical indicator holder connected to the connection hub). Examples motors and other movement imparting devices include, but are not limited to, a stepper motor, inductive motor, magnetically driven component, and any combinations thereof.

[0033] An aquatic environment analysis system, such as system 100, may include a chemical indicator holder cover having a fluid flow chamber. Such a chemical indicator holder cover may be additional to and/or used in combination with a flow control feature cover that covers one or more reservoirs of a chemical indicator holder. A chemical indicator holder cover is a physical apparatus designed to connect with a chemical indicator holder. In one exemplary aspect, a chemical indicator holder cover with a fluid flow chamber can provide a mechanism for delivering a flow of a fluid from an aquatic environment (e.g., an aquatic environment into which at least a part of a chemical indicator holder and chemical indicator holder cover to which it is connected are located, such as via submersion) and/or one or more desired improvements to such a flow. Examples of such an improvement to flow include, but are not limited to, directing flow into a reservoir of a chemical indicator holder, directing flow to a chemical indicator holder to flush a calibration solution from a reservoir, directing flow to a chemical indicator holder and out of a chemical indicator holder to keep a relatively fresh portion of fluid from an aquatic environment flowing across one or more chemical indicators, reduction of turbulence of flow (e.g., caused by movement of a chemical indicator holder), flow shaping, and any combinations thereof. In on example, a chemical indicator holder cover includes a fluid flow chamber that is sized, shaped and configured to hold a desired volume of fluid from an aquatic environment and, optionally, provide one or more improvements of flow. In such an example, a holder cover may also include a fluid flow inlet sized, shaped, and configured to promote fluid flow from an aquatic environment to flow into a corresponding fluid flow chamber. In such examples, a holder cover may also include a fluid flow outlet sized, shaped, and configured to promote fluid flow from a corresponding fluid flow chamber into a space (e.g., a reservoir) between the holder cover and one or more chemical indicators of a chemical indicator holder to which the holder cover is connected. A fluid flow inlet and/or fluid flow outlet may each also be sized, shaped, and/or configured to provide an improvement to flow (e.g., for improving flow into the space between a holder cover and into contact with one or more chemical indicators of a connected holder and then out of that same space via an opening formed in the connection between a structure of the holder cover and the holder). In one such example, such an opening is formed at an interface (e.g., at a position at least at a portion of a peripheral edge of the holder cover and/or holder) between a structure of the holder cover and a structure of the holder.

[0034] A connection between a chemical indicator holder and a corresponding chemical indicator holder cover may be by any of a variety of ways and/or physical mechanisms. A connection between a chemical indicator holder and a corresponding chemical indicator holder cover may also include connectivity of the holder to a corresponding monitoring/measuring apparatus (e.g., via a hub structure of a monitoring/measuring apparatus as disclosed in examples herein). In one example, a holder cover is removably attachable to a holder. In one such example, a holder cover is provided to an end user along with a corresponding optical sensing portion and one or more holders may be provided to the end user separately (e.g., the end user can attach the holder cover with the holder prior to use). In another example, a holder cover is connected in a fashion (e.g., with one or more fasteners, with one or more adhesives, with a not easily detached structural attachment, etc.) to a holder such that the holder cover and holder are not easily separated. In one such example, a holder and corresponding holder cover may be provided to an end user together (e.g., both being a consumable product that may be disposed upon depletion of any chemical indicators on the holder).

[0035] Examples of connectivity ways and physical mechanisms for connecting a holder and holder cover include, but are not limited to, a conformal fit of one or more structures of a holder cover with one or more structures of a holder, a pressure fit of one or more structures of a holder cover with one or more structures of a holder cover, a magnetic connection, a snap fit between one or more structures of a holder and one or more structures of a holder cover, a fastener fit (e.g., a connection utilizing one or more screws, rivets, and/or other fasteners, etc.) of one or more structures of a holder and one or more structures of a holder cover, an adhesive fit (e.g., a connection utilizing one or more adhesives) of one or more structures of a holder and one or more structures of a holder cover, a sharing or one or more components of a holder and one or more components of a holder cover (e.g., a holder and a holder cover are constructed of the same parts and/or material in a uniform fashion), a welding (e.g., an ultrasonic welding, a spin welding, a laser welding), and any combinations thereof. In one example, a holder cover includes one or more structural holder connection features (e.g., one or more posts) that extend from the holder and into corresponding one or more structures of a holder. In one such example, the one or more structural holder connection features of the holder cover extend through the holder and connect with one or more corresponding structures of a connection hub. In such a case, magnets of the chemical indicator holder cover may interact with magnets of the connection hub to form a magnetic connection of the holder cover to the holder to the hub. A magnetic connection and/or corresponding structural holder connection features of a chemical indicator holder cover may assist with providing connection to a monitoring/measuring apparatus, alignment of a chemical indicator with an optical reader, translation of motion to a chemical indicator holder (e.g., from a motor of a monitoring/measuring apparatus), and any combinations thereof. A structural holder connection feature (such as a post) may be configured and/or have a material selected to allow it to puncture or otherwise pass through a cover (e.g., a film cover, a foil cover, etc.) over a chemical indicator holder (e.g., over one or more connection slots of a holder into which a holder connection feature is to be inserted). [0036] FIG. 3 illustrates one exemplary embodiment of a method 300 of calibrating an aquatic environment analysis system (e.g., system 100, system 600 below, and other systems including components, apparatuses, etc. disclosed herein). At step 305, an optical reader (e.g., an optical reader of a monitoring/measuring apparatus according to the current disclosure) and a chemical indicator holder (e g., chemical indicator holder 110 or another holder of the current disclosure) is located in an aquatic environment. Locating the optical reader and the chemical indicator holder in an aquatic environment may occur in any way sufficient to allow for calibrating a chemical indicator and/or measuring a level of a predetermined constituent (e.g., as per any one or more of the embodiments disclosed herein). Example ways of locating an optical reader and chemical indicator holder in an aquatic environment include, but are not limited to, positioning one or more components of an aquatic environment analysis system in a fluid of an aquatic environment (e.g., such that a chemical indicator of a holder and an optical reader of a monitoring/measuring apparatus are exposed to the fluid at the appropriate time for calibration and/or measurement), having one or more components of an aquatic environment analysis system be made a part of or associated with a structure of a supporting equipment component of an aquatic environment (e.g., having a holder be removably connectable to a structure of an aquatic environment, such as a wall of a tank, and having an optical reader mounted to another structure of the environment, such as through a portal of a wall of a tank, in proper alignment with the holder to have the optical reader be capable of reading one or more chemical indicators of the holder), submersing one or more components of an aquatic environment analysis system in a fluid of an aquatic environment, placing one or more components of an aquatic environment analysis system in line with a flow of a fluid of the aquatic environment (e.g., in a supporting equipment component, such as a pipe or filter chamber), and any combinations thereof. It is noted that method 300 may, for example, utilize any aquatic environment analysis system disclosed herein, along with any suitable combination of relevant features, aspects, components, etc. of such a system as would be understood in light of the current disclosure.

[0037] At step 310, a chemical indicator of the chemical indicator holder is brought into alignment with the optical reader. Alignment generally is a position sufficient for the optical reader to take a reading of a physical characteristic of an aligned chemical indicator (e.g., to determine a change in the physical characteristic caused by a level of a constituent in a fluid of an aquatic environment, to calibrate the chemical indicator, etc ). Alignment can occur by any of a variety of ways. Example ways and/or mechanisms for aligning a chemical indicator and optical reader include, but are not limited to, connecting a removable chemical indicator holder in a position such that a chemical indicator is aligned with an optical reader, moving a chemical indicator holder with respect to a monitoring/measuring apparatus (e.g., using a motor and supporting structure(s)), moving a monitoring/measuring apparatus with respect to a chemical indicator holder (e.g., using a motor and supporting structure(s)), moving an optical reader with respect to a chemical indicator holder (e.g., using a motor and supporting structure(s), and any combinations thereof.

[0038] At step 315, a calibration reading is taken of the aligned chemical indicator while a calibration solution is in contact with one side of the chemical indicator. The calibration reading is taken using the optical reader through a space between the optical reader and the chemical indicator holder while a fluid from an aquatic environment is in the space. The calibration reading is taken from a side of the chemical indicator that is not the same side as the side in contact with the calibration solution (e.g., an opposite side of the chemical indicator). For example, a chemical indicator holder (e.g., made of a relevantly optically transparent material or having a window located for a chemical indicator) having a chemical indicator positioned thereon such that one side of the indicator faces in one direction away from a surface of the holder and the other side is positioned against the surface of the holder (and/or against a window of the holder) is read by an optical reader from the side against the surface of the holder. In one such example, the optical reader optically measures (e.g., via detection of light energy reflected or otherwise coming from the chemical indicator that is reactive to a level of a predetermined constituent that may or may not be present in the calibration solution) a physical characteristic of a chemical indicator. In another such example, the optical reader sends an excitation light energy to a chemical indicator and reads an emitted light energy (e.g., via fluorescence or other reaction type) from the chemical indicator. Any measured calibration reading and/or related data can be stored in a memory of the system for later use.

[0039] At step 320, a fluid of an aquatic environment (e.g., the same aquatic environment in the space between the optical reader and the holder) is allowed to come into contact with the side of the chemical indicator previously in contact with the calibration solution. In one example, the calibration solution that had been in contact with the chemical indicator is removed at some point prior to step 320. In another example, the calibration solution is removed from being in contact with the chemical indicator by a flow of the fluid of the aquatic environment. Removal of a calibration solution can occur by any of a number of ways including the many exemplary ways discussed in the current disclosure. For example, a calibration solution can be removed while a chemical indicator holder is in contact with a corresponding aquatic environment, a calibration solution can be removed while a chemical indicator holder is removed from and/or not in contact with a corresponding aquatic environment, and combinations thereof. In one example, a chemical indicator holder may be removably connected and/or positioned with respect to an optical reader (e.g., and corresponding monitoring/measuring apparatus) for putting into contact with a fluid of an aquatic environment (e g., in the space therebetween, in contact with a chemical indicator). In one such example, a chemical indicator holder is removed from contact with an aquatic environment (e g., to remove a cover or other feature retaining a calibration solution in contact with a chemical indicator) and calibration solution removed prior to being replaced in contact with the aquatic environment (e.g., to allow fluid of the aquatic environment to contact the chemical indicator.

[0040] At step 325, a measurement of a physical characteristic of the chemical indicator is read by the optical reader while the fluid of the aquatic environment is in contact with the side of the chemical indicator that was previously in contact with the calibration solution (“Contact Side”). The measurement is taken with the optical reader through a fluid of an aquatic environment in the space between the chemical indicator holder and the optical reader. In one exemplary aspect, the fluid in the space between the chemical indicator holder and the optical reader is not in contact with the chemical indicator in a fashion that allows the chemical indicator to be reactive to a predetermined constituent in the particular fluid in that space. The chemical indicator is reactive to the fluid from the aquatic environment that is in contact with the Contact Side of the chemical indicator. Any measured value and/or related data can be stored in a memory of the system for later use.

[0041] It is noted that a system may include a plurality of optical readers (e.g., having more than one optical reader as part of a monitoring/measuring apparatus associated with a chemical indicator holder) and that calibration readings (e.g., readings in step 31 ) and readings of a chemical indicator in contact with an aquatic environment (e.g., readings in step 325) may occur by different ones of the plurality of optical readers. In one example, a calibration reading is taken by a first optical reader and a later measurement of a level of a constituent in an aquatic environment is taken by a second optical reader of the same monitoring/measuring apparatus. In another example, a calibration reading and later reading of a level of a constituent in an aquatic environment are taken by the same optical reader of a monitoring/measuring apparatus. It is contemplated that step 320 and/or step 325 may occur at a time significantly after the calibration reading and removal of the calibration solution (e.g., at an iterative time after other chemical indicators are calibrated, at a time after other chemical indicators have been read in contact with an aquatic environment, etc.). [0042] At step 330, the measurement from step 325 is calibrated with the calibration reading from step 315 to obtain a value of a level of a predetermined constituent in the aquatic environment. Such a calibration of a measured value can occur in a variety of known ways. Example ways include, but are not limited to, changing a measured value to account for the calibrated reading, using a calibrated reading to change the manner in which a measured value is taken at the time taken (e.g., storing only a calibrated/adjusted value of a measured/ob served value at the time of observation by an optical reader), and any combinations thereof. Calibration of a measured value and/or determination of a level of a predetermined constituent can occur at a variety of times in relation to the time of observation/measurement of a value (e.g., in close time proximity to the time of observation/measurement, at a later time, etc.). The location of the storage of calibration and/or measurement data and any processing of the same can occur at one or more locations within a system (e.g., at a processing functionality of a monitoring/measuring apparatus, at a processing functionality of a different component of a system, at a user’s computing device, at a computing device located remotely (e.g., on the Internet or other cloud server), and any combinations thereof. Presentation of a level of a predetermined constituent to a user can occur via any display device associated (e g., wirelessly and/or wired connection) to a component of an aquatic environment analysis system).

[0043] FIG 4 illustrates another exemplary embodiment of a method 400 of calibrating an aquatic environment analysis system (e.g., system 100, system 600 below, and other systems including components, apparatuses, etc. disclosed herein). Method 400 is similar to method 300 and can include similar variations as discussed with respect to method 300 except as indicated below. At step 405, an optical reader (e.g., an optical reader of a monitoring/measuring apparatus according to the current disclosure) and a chemical indicator holder (e.g., chemical indicator holder 110 or another holder of the current disclosure) is located in an aquatic environment. At step 410, a chemical indicator of the chemical indicator holder is brought into alignment with the optical reader. At step 415, a calibration reading is taken of the aligned chemical indicator while a calibration solution is in contact with one side of the chemical indicator. The calibration reading is taken using the optical reader through a space between the optical reader and the chemical indicator holder while a fluid from an aquatic environment is in the space. The calibration reading is taken from a side of the chemical indicator that is not the same side as the side in contact with the calibration solution (e.g., an opposite side of the chemical indicator). At step 420, a fluid of an aquatic environment (e.g., the same aquatic environment in the space between the optical reader and the holder) is allowed to come into contact with the side of the chemical indicator previously in contact with the calibration solution. At step 425, a measurement of a physical characteristic of the chemical indicator is read by the optical reader while the fluid of the aquatic environment is in contact with the side of the chemical indicator that was previously in contact with the calibration solution (“Contact Side”). The measurement is taken with the optical reader through a fluid of an aquatic environment in the space between the chemical indicator holder and the optical reader.

[0044] At step 430, a second calibration reading is taken using an optical reader, the calibration reading being of a fluid in the space between an optical reader and the corresponding chemical indicator holder (e.g., using a reference location, such as a reference patch, of the chemical indicator holder). Such a calibration reading can assist in correcting for optical and other interferences (that may impact optical readings) caused by the fluid (and/or materials therein) of the aquatic environment that is in that space. Such a second calibration reading may occur at any time prior to step 440 below (e.g., prior to step 415, prior to step 420, prior to step 425, after step 425, etc.).

[0045] At step 440, the measurement from step 425 is calibrated with the calibration reading from step 415 and the calibration reading from step 430 to obtain a value of a level of a predetermined constituent in the aquatic environment.

[0046] FIG 5 illustrates another exemplary embodiment of a method 500 of calibrating an aquatic environment analysis system (e.g., system 100, system 600 below, and other systems including components, apparatuses, etc. disclosed herein). Method 500 may include similar variations as discussed with respect to method 300 and method 400 except as indicated below. At step 505, a chemical indicator of a chemical indicator holder is brought into alignment with an optical reader at a time when a reservoir of the chemical indictor holder has a calibration solution in contact with the chemical indicator. At step 510, a fluid of an aquatic environment is allowed to enter a space between the optical reader and the chemical indicator holder (e.g., via a flow of the aquatic environment, such as an inline flow being opened to enter the space; via an opening between the chemical indicator holder and a monitoring/measuring apparatus having the optical reader, etc.). At step 515, a calibration reading is taken of the aligned chemical indicator while the calibration solution is in contact with one side of the chemical indicator. The calibration reading is taken using the optical reader through a space between the optical reader and the chemical indicator holder while a fluid from an aquatic environment is in the space. The calibration reading is taken from a side of the chemical indicator that is not the same side as the side in contact with the calibration solution (e.g., an opposite side of the chemical indicator). At step 520, the calibration solution is removed from the reservoir. At step 525, a fluid of an aquatic environment (e.g., the same aquatic environment in the space between the optical reader and the holder) is allowed to come into contact with the side of the chemical indicator previously in contact with the calibration solution. At step 530, a measurement of a physical characteristic of the chemical indicator is read by the optical reader while the fluid of the aquatic environment is in contact with the side of the chemical indicator that was previously in contact with the calibration solution (“Contact Side”). The measurement is taken with the optical reader through a fluid of an aquatic environment in the space between the chemical indicator holder and the optical reader. At step 535, the measurement from step 530 is calibrated with the calibration reading from step 515 (e.g., and other calibration reading(s) as desired, such as a calibration related to the water between the optical reader and holder) to obtain a value of a level of a predetermined constituent in the aquatic environment.

[0047] It is noted that any calibration reading (e.g., of a chemical indicator, of a reference patch, of another type of mechanism for taking a calibration reading of a fluid in the space between an optical reader and a chemical indicator holder, etc.) and/or any measurement taken of a chemical indicator to determine a physical change when the indicator is in contact with a fluid of an aquatic environment may include taking multiple individual measurements (e.g., from the same spot on a chemical indicator or reference patch, from different spots on a chemical indicator or reference patch, using different optical readers, etc.). Results of multiple measurements may be combined (e.g., via averaging) for use in calibrating and/or determining a value of a level of a constituent in an aquatic environment.

[0048] FIG. 6 illustrates another example of an aquatic environment analysis system 600. System 600 includes a monitoring/measuring apparatus 605 having an upper portion 610 and a lower portion 615 that together enclose an internal space in which components of a monitoring/measuring apparatus (e.g., a motor to drive motion of a connected chemical indicator holder, one or more magnets to drive motion of a connected chemical indicator holder, connective circuitry, one or more components of an optical reader, one or more magnets for connecting the monitoring/measuring apparatus to a structure of an aquatic environment, a data connection to a processing functionality, a wireless circuitry, a power supply, a control circuitry (such as to control an optical reader, to control a motor, etc.) and any combinations thereof) may be located inside in whole or in part. The monitoring/measuring apparatus is shown magnetically connected to a mounting apparatus 620 which includes one or more magnets (not shown) inside a housing, the one or more magnets being sized, selected, and configured to magnetically couple to one or more magnets within monitoring/measuring apparatus 605 through a wall (e.g., a glass wall, an acrylic wall, a plastic wall, etc.) 625 of a structure of an aquatic environment. For example, apparatus 605 is located inside an aquatic tank on the side of wall 625 having a water containing fluid and mounting apparatus 620 is located on a dry side of wall 625.

[0049] Monitoring/measurement apparatus 605 is shown with an input wire 630 that is connected to an opening in lower portion 615 (e.g., a potted water-tight opening). An input wire, such as input wire 630, may include a power connection for connecting one or more internal components of apparatus 605 to a power supply, a data connection for connecting one or more components of apparatus 605 to an external component of system 600 (e.g., an external processing apparatus) for transferring data to the external component (e.g., from an optical reader, etc ), or other wired connection for connecting an external apparatus or device to monitoring/measuring apparatus 605. In another example, a monitoring/measuring apparatus may include a wireless power (e.g., induction driven wireless power) circuitry for receiving a wireless power input and/or a wireless communication circuitry for receiving/transmitting one or more data from/to another component or device.

[0050] System 600 includes a chemical indicator holder (not shown) that is positioned connected to a chemical indicator holder cover 635. The holder and holder cover 635 are shown connected to monitoring/measuring apparatus 605 with a lower surface (not shown) of the chemical indicator holder positioned proximate an upper surface of upper portion 610 of apparatus 605. Holder cover 635 includes a raised structural section 640 that includes a fluid flow inlet 645. Fluid flow inlet 645 is configured with three openings that are configured to allow fluid of an aquatic environment to flow into a fluid flow chamber that is located internal to holder cover 635. Holder cover 635 and the internal fluid flow chamber are configured to allow fluid to flow (e.g., via a fluid flow outlet of holder cover 635) from the chamber to a space above one or more chemical indicators of a chemical indicator holder connected thereto (e.g., via a reservoir of the holder). Additional features and aspects of various components of system 600 are discussed further with respect to exemplary components of exemplary system 700 of FIG. 7 below.

[0051] FIG. 7 illustrates a cross sectional view of another example of an aquatic environment analysis system 700. System 700 is similar to system 600 of FIG. 6 and includes exemplary components illustrative of features and aspects of the various components that may be included in an aquatic environment analysis system (such as system 100, system 700, etc.) even if one or more components is different in one way or another (different shape, structure, configuration, etc.). A person of ordinary skill will recognize the general applicability of the details in the discussion of the components of system 700 to other differently configured components of different aquatic environment analysis systems.

[0052] System 700 includes a monitoring/measuring apparatus having a bottom portion 702 and a top portion 704. Bottom portion 702 and top portion 704 enclose an internal space that includes a motor 708. System 700 also includes a chemical indicator holder 710 having one or more chemical indicators 712 within one or more reservoirs 714. System 700 also includes a chemical indicator holder cover 718 having a fluid flow chamber 720. The cross section shown in FIG. 7 is at a location in system 700 and holder cover 718 such that it cross sections a structural rise 722 in an outer wall of holder cover 718 corresponding to a fluid flow inlet (not shown). Additional structural views of a holder cover similar to holder cover 718 are discussed further below with respect to FIGS. 9A to 9F. Holder cover 718 includes a lower structural portion 724 that is shaped and configured in conjunction with the shape and configuration of upper portions of holder 710 to be in contact with holder 710 and to form an at least partial conformal fit with upper surfaces of holder 710 to enclose reservoir 714. It is noted that the at least partial conformal fit may not be a water-tight or air-tight closure of reservoir 714, but is designed to be a fit that is sufficient for controlling flow of a fluid within and out of reservoir 714 to a degree needed for interaction with one or more chemical indicators 712. For example, water from within reservoir 714 may flow over a top of a wall of reservoir 714 and into space 730 (discussed further below) (e.g., via flow of water into reservoir 714 providing pressure driving such flow, via rotational motion of holder 710, etc ). A chemical indicator holder, such as holder 710, may also include one or more flow ports (not shown in FIG. 7). One or more flow ports may be located on an inner portion of a holder, such as inside the donut shaped reservoir 714 of holder 710, and be shaped and configured to allow fluid to flow from the top side of the holder (e.g., out of a reservoir) and into a space below the holder (e.g., into space 730 below holder 710 and above top portion 702.

[0053] A space 730 is formed by the configuration and shape of holder 712, holder cover 718, and upper portion 702 when components are connected. An opening 735 that is located at peripheral edge 740 of holder cover 718 allows fluid from an aquatic environment to flow from outside system 700 into space 730. Space 730 extends below holder 712 and between holder 712 (and one or more chemical indicators 712) and a lens optic 745 of an optical reader. Upper portion 702 includes a fin protrusion 750. Fin protrusion 750 and other aspects of upper portion 702 are described further below with respect to FIGS. 9A to 9F. A fin protrusion of a monitoring/measuring apparatus, such as fin protrusion 750, that is located proximate a surface near where a chemical indicator holder is located may provide a functionality including, but not limited to, assisting with fluid flow around a holder, assisting with fluid flow in a space between a holder and an optical reader, limiting fluid turbulence, and any combinations thereof.

[0054] System 700 also includes a movable magnet component 755 having one or more magnets 760. Movable magnet component 755 is located within the volume created by upper portion 702 and lower portion 704 and is connected to motor 708 via a rotational axis 765. Rotational axis 765 translates rotational movement to movable magnet component 760, which rotates the one or more magnets 760. A power supply connection 770 provides power to internal components of the monitoring/measuring apparatus (e.g., optical reader, motor 708, a control and/or processing circuitry 775, etc.).

[0055] System 700 also includes a connection hub 780 that has one or more magnets (not shown in this cross section). Hub 780 is connected to the outside of upper portion 702 in a configuration that allows hub 780 to rotate freely and as motion is magnetically transferred to hub 780 through upper portion 702 from movable magnet component 755.

[0056] System 700 includes optional connection magnets 795 that are designed and configured to mount system 700 to a surface of an aquatic environment (e.g., through a wall of the aquatic environment to another set of magnets on the other side of the wall, to magnets or magnetic material of a wall of an aquatic environment, through a wall of the aquatic environment to a mounting apparatus having one or more magnets configured to magnetically connect to magnets 795, etc.).

[0057] In one example of operation, holder cover 718 includes one or more magnets and/or other structures that connect to holder 710 and hub 780 structurally and/or magnetically. Motor 708 can provide rotary motion to holder 710 (and holder cover 718) via rotation of axis 765 and movable magnet component 755, which magnetically imparts rotation to hub 780. In one example, holder 710 is provided to a user with a calibration solution in reservoir 714. An opening 798 to reservoir 714 would include in such an example a cover or other feature to retain the calibration solution.

Rotational movement of a chemical indicator holder, such as holder 710, may provide a function that includes a function including, but not limited to, moving a holder to align a chemical indicator with an optical reader, moving a holder to impart a force to impact a flow of a fluid with respect to a reservoir or other proximate space of a holder, moving a holder to induce a flow of a fluid into a fluid flow input of a holder cover, moving a holder to induce a flow of a fluid through a fluid flow output of a holder cover, moving a holder to induce a flow of a fluid in a reservoir, and any combinations thereof. A calibration reading may be taken using lens 745 and corresponding optical reader components (e.g., sensor, circuitry, etc.) from one or more chemical indicators 712 while a calibration solution is in reservoir 714 (e.g., while a fluid of an aquatic environment is located in space 730, but not in reservoir 714 and not interacting with one or more chemical indicators 712. Such a calibration reading is taken through the fluid in space 730. After a calibration reading is taken, the calibration solution can be removed from reservoir 714 (e.g., by removing holder 710, removing any cover or other feature over opening 798, etc.). In an example of operation, holder 710 (e.g., reconnected after removing calibration solution) may be rotated to induce a flow of fluid from an aquatic environment into a fluid flow inlet of holder cover 718 and into fluid flow chamber 720, through a fluid flow outlet (e.g., in lower portion 724 of holder cover 718) and into reservoir 730 to come into contact with one or more chemical indicators 712. The one or more chemical indicators 712 are then put into alignment (via movement from motor 708) with lens 745 so that a value of a physical characteristic of the one or more chemical indicators can be determined using lens 745 and optical reader components. Data of such a value can then be processed to determine a level of a predetermined constituent of the fluid of the aquatic environment. If one or more calibration measurements exist, such one or more calibration measurements can be utilized in the process of determining the level of the predetermined constituent.

[0058] FIG. 8 illustrates an exemplary exploded view of various components of system 700 of FIG. 7. FIG. 8 shows lower portion 704 and upper portion 702 of a monitoring/measuring apparatus, connection hub 780, holder 710, lower portion 724 of holder cover 718, and holder cover 718. Additional views and details of each of these components are described further below.

[0059] FIGS. 9A to 9F show additional views of an upper portion of chemical indicator holder cover 718. FIG. 9A shows a side view of the upper portion of holder cover 718, FIG. 9B shows a perspective view of the upper portion of holder cover 718, FIG. 9C shows a bottom view of the upper portion of holder cover 718 (without lower structural portion 724), and FIGS. 9D to 9F show various cross-sectional views of the upper portion of holder cover 718 (without lower structural portion 724). Lower structural portion 724 is discussed further with respect to FIGS. 10A to 10F below in part to show features of lower structural portion 724 that are hidden from view when lower structural portion 724 is connected or otherwise made a part of the upper portion of holder cover 718 (e.g., by physical connections, such as snap connectors and/or screws, or by being made an integral part of the upper portion). Holder cover 718 includes a sloped heightened upper wall and surface 905. Upper wall and surface 905 progressively slope upwardly around the outside of holder cover 718 to near structural rise 722 and a fluid flow inlet 910. Fluid flow inlet 910 includes three openings 915 that are sized, shaped, and configured to allow for fluid from an aquatic environment to flow through openings 915 into fluid flow chamber 720 that is formed between upper wall and surface 905 and lower structural portion 724 (described further below). Holder cover 718 includes a side wall 920 around its circumference that extends downward from upper wall/ surface 905 and includes a plurality of structural fins 925. Structural fins 925 are shaped, sized, and configured to create a turbulence in a fluid surrounding holder cover 718 (e.g., a fluid of an aquatic environment into which holder cover 718 is submersed) via rotational movement of holder cover 718 when rotated along with corresponding holder 710 (see description of such translation of motion from motor 708 to holder 710 and holder cover 718).

[0060] Structural fins 925 flare outwardly at different degrees along their length of the peripheral edge 930 of holder cover 718. Holder cover 718 also includes one or more connection ports 935 sized and configured to allow a portion of lower structural portion 724 to be connected thereto. Holder cover 718 further includes an optional plurality of fluid vent holes 940. Fluid vent holes 940 are sized, shaped, and configured to allow fluid from within fluid flow chamber 720 to escape to outside holder cover 718 (e.g., into a surrounding aquatic environment) under predetermined conditions of flow pressure, such as when a fluid flow exceeds a level that may force a connected chemical indicator holder (e.g., holder 710) to disconnect from holder cover 718 (e.g., via fluid flow out of a corresponding fluid flow outlet and into a reservoir of the holder. Such an increase in fluid flow pressure may be caused by a variety of causes such as, for example, a temporary spike in aquatic environment flow caused by a pump or other supporting equipment component of the aquatic environment. The size, shape, and configuration of fluid vent holes 940 is such that under normal operation of holder cover 718 and corresponding components, fluid flow in fluid flow chamber 720 is more likely to flow out the corresponding fluid flow outlet into a space above a connected chemical indicator holder. In one example, one of more fluid vent holes (e.g., holes 940) in a holder cover may prevent a holder cover from disconnecting from a connected holder and/or monitoring/measuring apparatus if a pressure of a fluid flow increases beyond a certain point (e.g., where such pressure overcomes a structural and/or magnetic connection of a holder cover to another component). [0061] FIGS. 10A to 10F show additional views of lower structural portion 724 of holder cover 718. FIG. 10A shows a bottom view of lower structural portion 724, FIG. 10B shows a bottom perspective view of lower structural portion 724, FIG. 10C shows a top-down perspective view of lower structural portion 724, and FIGS. 10D to 10F show various cross sectional views of lower structural portion 724. Lower structural portion 724 includes a lower surface 1005 and an upper surface 1010. Extending from lower surface 1005 are four structural holder connection features in the form of posts 1015, 1020, 1025, and 1030. Posts 1015, 1020, and 1025 have similar size and shape (e.g., a rounded cross section with each having a similar length dimension). Post 1030 has a different cross-sectional shape from posts 1015, 1020, and 1025. Such a different shape or alternative different configuration of one of a plurality of structural holder connection features can create a keying functionality that when combined with structural connection holes or recesses in another component that are shaped, sized, and configured to match with the keyed structural holder connection feature (e.g., a differently shaped post) can ensure that components are connected together in a predetermined orientation and/or position (e.g., for alignment of subcomponent features and aspects from one component to another).

[0062] A structural holder connection feature, such as posts 1015, 1020, 1025, 1030, can be made of a variety of materials and configurations. A structural holder connection feature may be a solid material feature. A structural holder connection feature may be a hollow material feature. A structural holder connection feature may include and/or be located proximate to one or more magnets. Each of posts 1015, 1020, 1025, and 1030 includes a corresponding hollow section 1035, 1040, 1045, 1050. A magnet (not shown) is positioned in each of hollow sections 1035, 1040, 1045, 1050 and is sized and configured to match with one or more magnets (discussed below of connection hub 780.

[0063] Lower structural portion 724 also includes a raised structure 1060 having a sloped side and an opening that forms a fluid flow outlet 1065 that is configured to promote flow of a fluid from within fluid flow chamber 720 to a space between lower surface 1010 and an upper surface of a connected holder (e.g., reservoir 714 of holder 710). Lower structural portion 724 also includes another raised structure 1070 that is shaped, sized, and configured to create a fluid flow recess 1075 in lower surface 1005. Recess 1075 is sized to extend inwardly in surface 1005 beyond opening 798 of reservoir 714 when holder 710 is connected to lower structural portion 724 of holder cover 718 such that fluid flow can be promoted from reservoir 714 toward a central portion of holder 710 (discussed further below). [0064] FIGS. 11 A to 11G show additional views of chemical indicator holder 710. FIG. 11A shows a top view of holder 710, FIG. 1 IB shows a top perspective view of holder 710, FIG. 11C shows a bottom view of holder 710, FIG. 1 ID shows a bottom perspective view of holder 710, FIGS. 1 IE to 11G show various cross-sectional views of holder 710 from different directions and locations in holder 710. Holder 710 includes a plurality of rectangular mounting locations 1105 arranged on a lower surface of reservoir 714. Rectangular mounting locations 1105 include a rectangular raised feature creating a rectangularly bordered area of the lower inside surface of reservoir 714. Holder 710 includes eleven such rectangular mounting locations 1105. Each rectangular mounting location may include a chemical indicator (e.g., in the form of a chemical indicator patch adhered to or otherwise positioned on the surface of holder 710). In one example, holder 710 is constructed of a material that includes a material at each of the rectangular mounting locations 1105 that is optically transparent and has limited emission (e g., fluorescence) at wavelengths of energy relevant for a corresponding optical reader to read select chemical indicators to be included at each of the rectangular mounting locations. It is noted that specific regions and/or demarcations of the same similar to those associated with rectangular mounting locations 1105 are not a requirement of a chemical indicator holder and holder without any such marked regions for chemical indicators are contemplated.

[0065] Holder 710 includes reservoir 714 which is in the shape of a semi-donut-like trough formed by lower wall having an inner surface 1110 and an outer surface 1115. An outer wall 1118 having an inner surface 1120 and an outer surface 1125 and an inner wall 1128 having an inner surface 1130 and an outer surface 1135 each rise up from the lower wall to partially enclose reservoir 714 and any chemical indicators included at one or more of locations 1105. A lip 1140 extends from the top of outer wall 1118. Holder 710 includes a raised section 1145. Raised section 1145 is part of a flow receiving feature 1150 that in holder 710 has a slope downwardly from raised section 1145 to the lower wall of reservoir 714 in a manner to create a sloping surface that merges to inner surface 1110 of the lower wall. On the other side of raised section 1145 is a flow ejection feature 1155 that in holder 710 has a slope upwardly from inner surface 1110 of the lower wall of reservoir 714 to the top of raised section 1145. A flow receiving feature of a holder (such as that formed by feature 1150 and raised section 1145) is an optional feature of a holder shaped, sized, and configured to receive a flow of a fluid of an aquatic environment from a fluid flow outlet of a holder cover (e.g., fluid flow outlet 1065). A flow ejection feature of a holder (such as that formed by feature 1155 and raised section 1145) is an optional feature of a holder shaped, sized, and configured to direct fluid flow out of a reservoir of a holder (e.g., over a side wall of a reservoir, over a side of a holder, out of an opening of a space between a holder and a holder cover, into a fluid flow recess of a holder cover (such as recess 1075), and any combinations thereof). In exemplary holder 710, the shape, size, and configuration of raised section 1145, flow receiving feature 1150, and flow ejection feature 1155 are such that fluid flow enters reservoir 714 at the side of raised section 1145 having flow receiving feature 1150 and is directed to flow through reservoir 714’s donut-like shape counterclockwise (looking down into reservoir 714) to the side of raised section 1145 having flow ejection feature 1155. The sloped aspect of flow receiving feature 1150 assists in directing flow through reservoir 714 and the sloped aspect of flow ejection feature 1155 assists with directing flow upward and out of reservoir 714. It is noted that other shapes and configurations other than a sloping aspect may be utilized in a holder for flow receiving and flow ejection features. Additionally, such features may be omitted from a holder.

[0066] Holder 710 includes a central region having an upper side 1160. Four connection slots 1165, 1170, 1175, 1180 are located such that they form an opening through the central region of holder 710. A connection slot of a holder, (e.g., connection slots 1165, 1170, 1175, 1180) is sized, shaped, and configured to receive a corresponding holder connection feature of a holder cover (e.g., posts 1015, 1020, 1025, and 1030 of holder cover 718) and allow such holder connection feature to pass from the upper side 1160 to the opposite side of holder 710 (e.g., to connect to a component of a monitoring/measuring apparatus). A holder may include a different number of differently shaped, sized, and configured connection slots than that of holder 710 (e.g., having one or more connection slots matched to corresponding one or more holder connection features and/or one or more aspects of a component of a monitoring/measuring apparatus) or alternatively include no connection slots at all. Connections slots 1165, 1170, and 1175 have similar shapes and sizes that match with posts 1015, 1020, and 1025 and connection slot 1180 has a shape and size to match post 1030 (i.e., the keying post). The latter keying of post 1030 to fit only in connection slot 1180 due to its different shape/size assists with aligning holder 710 with holder cover 718 in a predetermined position (e.g., aligning raised section 1145 between fluid flow outlet 1065 and fluid flow recess 1075).

[0067] Holder 710 includes an optional optic cleaner connection point 1185 configured to have a cleaning element (e g., a brush) attached that is configured to clean an optical component of an optical reader of a connected monitoring/measuring apparatus. Various examples of optical reader cleaning elements, such as brushes, and related details are disclosed in the ‘281 Patent, the disclosure of which related to the same is incorporated herein by reference. [0068] Holder 710 may include a cover (not shown) or other flow controlling feature over opening 798 of reservoir 714. In one example, holder 710 includes a removable foil and/or film cover over opening 798 and a calibration solution in reservoir 714 in contact with one or more chemical indicators of holder 710. For example, such a removable cover may be on a holder with calibration solution therein when a holder is first received by a user and be removable (all or in part) to remove the calibration solution (e.g., after taking one or more calibration readings of chemical indicator(s) with the calibration solution in contact). Examples of a holder with a cover thereon are illustrated below with respect to FIGS. 14A to 14C and FIGS. 15A to 15D.

[0069] A holder may include one or more fluid flow vents sized, shaped, and configured to allow fluid (e.g., of an aquatic environment) to flow from a side of the holder having one or more chemical indicators to a side of the holder that is to be positioned proximate an optical reader. Holder 710 includes six fluid flow vents 1190 (noting that not all six are numbered in the figures with lead lines) that are pill shaped and located in the central region of holder 710 and are configured to allow fluid to flow from side 1160 to the underside of holder 710 (e.g., into space 730 between holder 710 and upper portion 702 of the monitoring/measuring apparatus). In one example, such a fluid flow comes out of reservoir 714 via flow ejection feature 1155 and fluid flow recess 1075 that spans from reservoir 714 across inner wall 1128 to the top side of central region of holder 710 (e.g., between holder 710 and lower structural portion 724 when holder 710 and holder cover 718 are connected).

[0070] A holder, such as holder 710, (and chemical indicators thereon) may be designed to be disposable (e.g., after a period of time). A limited lifespan of a chemical indicator holder may be due to a limited userfulness of one or more of the chemical indicators of the holder (e.g., due to optical degradation, due to chemical degradation, and combinations thereof). A chemical indicator holder of the current disclosure may be made to be easily removable to aid in changing to a new holder. Additionally, a chemical indicator holder may be made to be retained as part of a system without being disposable. In one example, a holder may be easily connectible to a holder cover (and also to a monitoring/measuring apparatus or other system connection) via a magnetic connection between a holder cover and a component of a monitoring/measuring apparatus without the holder having magnets (e.g., or magnetic materials). In one exemplary aspect, such a configuration without magnets may reduce manufacturing expenses related to a disposable holder and/or provide environmental benefits relating to disposal. [0071] FIGS. 12A to 12F show additional views of connection hub 780. FIG. 12A shows a top view of connection hub 780, FIG. 12B shows a top perspective view of connection hub 780, FIG. 12C shows a bottom view of connection hub 780, FIG. 12D shows a bottom perspective view of connection hub 780, FIG. 12E shows a cross sectional view of connection hub 780, and FIG. 12F shows a close-up cross-sectional view of a portion including a structural recess 1205. Connection hub 780 includes four structural recesses 1205, 1210, 1215, and 1220. Structural recesses 1205, 1210, and 1215 are similarly sized, shaped, and configured to receive corresponding ones of the ends of posts 1015, 1020, and 1025 of holder cover 718. Recess 1220 is sized, shaped, and configured to receive an end of post 1030 of holder cover 718. Connection hub 780 includes one or more magnets at each of magnet locations 1225, 1230, 1235, 1240 (e.g., encased in a material of connection hub 780) that are located below structural recesses 1205, 1210, 1215, and 1220. Magnets and corresponding magnet locations 1225, 1230, 1235, 1240 (along with configuration of the same) are selected to provide a magnetically sufficient connection through the material of structural recesses 1205, 1210, 1215, and 1220 to connect to magnets in the hollow sections 1035, 1040, 1045, 1050 of posts 1015, 1020, 1025, 1030 of chemical indicator holder cover 718.

[0072] In one example of usage, holder 710 is connected to holder cover 718 by inserting posts 1015, 1020, 1025, 1030 into connection slots 1165, 1170, 1175, 1180 of holder 710 (e.g., aligning the keying post 1030 with similarly configured connection slot 1180) and then connecting holder 710 and holder cover 718 to connection hub 780 by aligning posts 1015, 1020, 1025, 1030 with corresponding ones of structural recesses 1205, 1210, 1215, and 1220. In such an example, magnets in each of hollow sections 1035, 1040, 1045, 1050 magnetically connect with corresponding ones of magnets in magnet locations 1225, 1230, 1235, 1240.

[0073] Additional views of upper portion 702 along with a discussion of details are illustrated in FIGS. 13A to 13G. FIG. 13 A shows a top-down view of upper portion 702, FIG. 13B shows a bottom view of upper portion 702 (i.e., the side of upper portion 702 that is inside the monitoring/measuring apparatus facing toward bottom portion 704 and the interior space of the apparatus), FIG. 13C shows an top side perspective view of upper portion 702, and FIGS. 13D to 13G show various cross sectional views of upper portion 702 from different angles and locations. A top view 1305 of lens optic 745 of a first optical reader and a top view 1310 of a lens optic of a second optical reader are shown from the top side of upper portion 702 (e.g. each positioned through a circular opening in the structure of upper portion 702). A bottom view 1315 of an underside of lens optic 745 and a bottom view 1320 of an underside of the lens optic of the second optical reader are also shown. Additional ports/openings 1325 and 1330 in upper portion 702 are also shown and are sized and configured to include one or more non-optical reading sensors (e.g., a conductivity electrode, a temperature sensor, etc.). Data from such sensors may be used to report to a user of an aquatic environment analysis system and/or in a calculation of a value for a level of a predetermined constituent in an aquatic environment.

[0074] Upper portion 702 includes a fin 1335 having an upper ridge 1340. A fin such as fin 1335 can be shaped and configured to block light from around an aquatic environment analysis system from entering a lens of an optical reader (e.g., limiting interference from such light with measurements/readings by the optical reader of light/energy from a chemical indicator), block light from around an aquatic environment analysis system from being directed onto a chemical indicator or other component of the system (e.g., limiting possible unintended fluorescence or other excited emission), control flow of fluid of an aquatic environment in a space (e.g., space 730) between a chemical indicator holder and a monitoring/measuring apparatus, for other purposes, and any combinations thereof. Fin 1335 only partially extends around the surface of upper portion 702 with the height of ridge 1340 sloping downward to the surface of upper portion 702 and having a portion of the inner circumference of that surface have lesser to no portion of fin 1335 thereby creating a passage for fluid flow to the outside periphery of the space 730 and through opening 735 away from the system.

[0075] Upper portion 702 includes a hub connection structure 1350 that is shaped, sized, and configured to connect with connection hub 780 (e.g., via a rotary structure, such as a set of bearings, that allow connection hub 780 to rotate around hub connection structure). Upper portion 702 also includes a structural recess 1355 in its underside that is shaped, sized, and configured to have moveable magnet component 755 rotate therein (e.g., via translation of motion from motor 708).

[0076] FIGS. 14A to 14C illustrate yet another example of a chemical indicator holder 1405. Chemical indicator holder 1405 has a similar shape and configuration as chemical indicator holder 710 discussed above. FIG. 14A shows a top-down view of chemical indicator holder 1405 having a foil cover 1410 over an opening of a reservoir of holder 1405 (not visible in this view). In this example, foil cover 1410 is shown extending fully over holder 1405 and its donut-shaped reservoir and central region having a plurality of connection slots similar to those of holder 710 above. FIG. 14B shows a bottom view of chemical indicator holder 1405, which is made of an optically transparent material (e.g., is transparent to visible light viewable by the human eye as well as relevant wavelengths of light for its corresponding optical reader(s) and chemical indicator(s)). Foil cover 1410 shown behind holder 1405 can be seen through holder 1405 and extending beyond the peripheral circumference of holder 1405. A calibration solution is enclosed in a reservoir (similar to reservoir 714 of holder 710) of holder 1405 and retained from leaving from the opening of the reservoir covered by foil cover 1410 by foil cover 1410. It is noted that the calibration solution is clear and not easily visible in FIG. 14B. FIG. 14C shows holder 1405 after holder 1405 has been connected to a holder cover (similar to holder cover 718 discussed above) having a plurality of structural connection features/posts (not shown). Foil cover 1410 is shown with a plurality of puncture holes 1415 aligned with the connection slots of holder 1405 that were created by the connection features/posts of the holder cover protruding through the connection slots (e.g., and connected to a hub or other component of a monitoring/measuring apparatus for taking one or more calibration readings of one or more chemical indicators of holder 1405 while a calibration solution is in contact with the one or more chemical indicators). Foil cover 1410 is also shown partially removed from holder 1405 (i.e., peeled back) to reveal a portion of an opening 1420 to the reservoir of holder 1405 and to allow the calibration solution therein to be removed. Foil cover 1410 can be fully removed. After removal of the calibration solution, holder 1405 may be repositioned/reconnected to its holder cover and corresponding monitoring/measuring apparatus for taking one or more measurements of a level of a constituent in an aquatic environment as disclosed herein.

[0077] FIGS. 15A to 15D illustrates still another example of a chemical indicator holder 1505. Chemical indicator holder 1505 has a similar shape and configuration as chemical indicator holder 710 discussed above. Holder 1505 includes a plurality of chemical indicators enclosed in a partially donut-shaped reservoir that is similar to reservoir 714 of holder 710. An opening of the reservoir of holder 1505 is covered by a clear film cover that encloses a clear calibration solution 1515 in the reservoir and in contact with the plurality of chemical indicators. FIG. 15A shows a top view of holder 1505 looking down on film cover 1510. FIG. 15B shows a top perspective view of holder 1505 showing an air bubble under film cover 1510 illustrating the presence of calibration solution 1515. FIG. 15C illustrates a bottom view of holder 1505 looking down on an optically transparent lower wall 1520 of the reservoir of holder 1505 and through to the bottom sides of the plurality of chemical indicators. Holder 1505 includes connection slots 1525, 1530, 1535, 1540 and an optical reader cleaning brush 1545 (connected to a connection point on the bottom of holder 1515 and configured to contact a portion of an optical reader when holder 1505 is rotated with respect to the optical reader). The plurality of chemical indicators include an indicator patch reactive to measure pH 1550, two indicator patches reactive to measure ammonia 1555 and 1560, and two indicator patches reactive to measure oxygen (e.g., dissolved oxygen) 1565 and 1570. A chemical indicator holder, such as holder 1505 and other holders of the current disclosure, may have multiple indicators of the same type (e.g., for redundancy, to extend the longevity of reading time for a particular holder, and combinations thereof). Holder 1505 also includes a fluorescent reference patch 1575, a white reference patch 1580, and a black reference patch 1585. Reference patches on a chemical indicator holder may be utilized for one or more calibrations using a corresponding optical reader (e.g., calibrating an optical reader and a fluid in a space between a holder and an optical reader). A holder according to the current disclosure may include any one or more reference patches (e.g., at or otherwise as part of an optical calibration reference location). Example reference patches include, but are not limited to, a black reference patch, a white reference patch, a fluorescence reference patch, and any combinations thereof. It is contemplated that an optical calibration location may include (in addition to and/or in place of a reference patch) a material configured for providing a calibration reading of the aquatic environment in a space between an optical reader and a holder (e.g., as part of a material of the holder itself). Holder 1505 has several locations, such as location 1590 that do not have a chemical indicator patch or a reference patch.

[0078] FIG. 15D shows chemical indicator holder 1505 with film cover 1510 partially removed to expose the reservoir of holder 1505 and the calibration solution therein (e.g., to remove the calibration solution). Film cover 1510 can be fully removed. After removal of the calibration solution, holder 1505 may be repositioned/reconnected to its holder cover and corresponding monitoring/measuring apparatus for taking one or more measurements of a level of a constituent in an aquatic environment as disclosed herein.

[0079] Example A

[0080] In one example, a holder for an aquatic environment analysis system includes a plurality of chemical indicators. One chemical indicator is configured to detect a presence of free ammonia.

Measuring free ammonia may assist with avoiding an emergency with an aquatic environment (e.g., free ammonia is a form of ammonia that can be toxic to aquatic life, such as fish in an aquarium). Measuring free ammonia as opposed to ionized ammonia, which can be slower to form in an aquatic environment, may allow a user to more quickly react to undesired levels of developed and/or developing ammonia in their environment (e.g., getting ahead of ammonia spikes). Continuous availability of ammonia measurements (e.g., as may be provided by one or more systems and/or methods of the current disclosure) may be useful in aquatic environment cycling and/or setup (e.g., cycling and/or setup of an aquarium tank) such as via tracking of progress and/or pace of cycling. For example, a system of the current disclosure may be configured (e.g., with an appropriate chemical indicator of a holder) for measuring free ammonia in a range of 0 to 100 parts per billion (ppb) as NH3. Another chemical indicator in this example is a chemical indicator configured to detect a presence of dissolved oxygen. Aquatic life (e.g., fish and coral) may rely heavily on dissolved oxygen depending on the aquatic environment. For example, a system of the current disclosure may be configured (e.g., with an appropriate chemical indicator of a holder) for measuring dissolved oxygen in a range of 0 to 20 mg/L and/or 0 to 20 ppm and/or a saturation amount of 0 to 100%). Yet another chemical indicator in this example is a chemical indicator configured to detect pH. The measurement of pH in an aquatic environment may be important in taking care of a consequential and/interconnected parameter for one or more of the aquatic life in the environment. For example, a system of the current disclosure may be configured (e.g., with an appropriate chemical indicator of a holder) for measuring pH in a range of 6.5 to 9.0. A system and/or method of the current disclosure may also be configured with an ability to measure salinity of an aquatic environment (e.g., having one or more electrodes, such as two maximum area electrodes (e.g., made of platinum and rhodium), associated with a system of the current disclosure and providing readings to be processed by a processing element of the system and related information provided to a user (e.g., via a display element). For example, a system of the current disclosure may be configured (e.g., with an appropriate set of electrodes of an optical sensing portion) for measuring a salinity in a range of 25 ppt to 40 ppt and/or 25 PSU to 40 PSU and/or 1.018 to 1.032 specific gravity. A system and/or method of the current disclosure may also be configured with an ability to measure temperature of an aquatic environment (e.g., having one or more temperature probes associated with a system of the current disclosure and providing readings to be processed by a processing element of the system and related information provided to a user (e.g., via a display element). For example, a system of the current disclosure may be configured (e.g., with an appropriate temperature probe of an optical sensing portion) for measuring temperature in a range of 16 degrees Celsius to 32 degrees Celsius.

[0081] Other Examples

[0082] It is noted that any of the following examples in this section that are methods may be implemented (in whole or in part) as a computer implemented method. It is contemplated that any of the details, concepts, aspects, features, characteristics, and/or alternatives of a component/element discussed with respect to the following examples in this section may be combined alone or in combination with others with one or more of the details, concepts, aspects, features, characteristics, examples, and/or alternatives of a component/element discussed elsewhere herein with respect to an aquatic environment analysis system (or one or more components thereof) and/or method (e.g., a method of calibrating an aquatic environment analysis system), as appropriate.

1. An aquatic environment analysis system comprising: an optical sensing portion, at least a portion of the optical sensing portion designed and configured to be in contact with a first aquatic environment and including a first optical reader; a holder having one or more chemical indicators, each of the one or more chemical indicators having a first side reactive for indicating a level of a predetermined constituent of the first aquatic environment and a second side opposite the first side, the holder being positioned with respect to the optical sensing portion such that the second side is positioned facing the first optical reader and the first side is positioned facing in a direction away from the first optical reader, the holder including: a first reservoir enclosing at least a portion of the first side of at least one indicator of the one or more chemical indicators; a calibration solution positioned inside the first reservoir; and a selectively openable opening designed and configured to selectively contain the calibration solution and allow the calibration solution to be removed and fluid from the first aquatic environment to enter the first reservoir; wherein the optical sensing portion and holder are positioned and configured with a first space between the first optical reader and the second side of the at least one indicator, the first space having at least one opening allowing a portion of the first aquatic environment to fill the first space when the optical sensing portion and the holder are at least partially submersed in the first aquatic environment and/or positioned in line with the first aquatic environment; and wherein the first optical reader is configured to take a first calibration reading from the at least one indicator while the calibration solution is positioned inside the first reservoir.

2. A system according to example 1, wherein the second side is treated to not be reactive to the level of a predetermined constituent. 3. A system according to any one of the preceding examples, wherein the holder includes a window proximate the second side, the window and/or the second side configured to: allow the optical reader to read one or more optical qualities of the at least one indicator through the window; and not allow the first aquatic environment to contact the at least one indicator through the window.

4. A system according to any one of the preceding examples, wherein the first optical reader is configured to take a second calibration reading of the portion of the first aquatic environment in the first space.

5. A system according to example 4, wherein the holder includes one or more optical calibration reference locations and the first optical reader is configured to take the second calibration reading using at least one of the one or more optical calibration reference locations.

6. A system according to any one of examples 6 and 5, wherein the one or more optical calibration reference locations includes a patch selected from the group consisting of a black reference patch, a white reference patch, a fluorescence reference patch, and any combinations thereof.

7. A system according to any one of the preceding examples, wherein the first optical reader is configured to take a second calibration reading of the portion of the first aquatic environment in the first space while the calibration solution is positioned inside the first reservoir.

8. A system according to any one of the preceding examples, wherein the first space is exposed to a fluid of a plumbing component and/or control system of the aquatic environment.

9. A system according to any one of the preceding examples wherein the holder includes a plurality of chemical indicators.

10. A system according to example 9, wherein two or more of the plurality of chemical indicators are included in a location of the holder such that the first side is at least partially enclosed by the first reservoir.

11. A system according to example 10, wherein the calibration solution is in contact with the two or more of the plurality of chemical indicators.

12. A system according to example 9, wherein the first reservoir includes two or more separate reservoir chambers.

13. A system according to example 12, wherein each of the two or more separate reservoir chambers includes one or more of the plurality of chemical indicators at least partially enclosed by the corresponding one of the two or more separate reservoir chambers. 14. A system according to any one of examples 12 and 13, wherein each of the two or more separate reservoir chambers includes a feature as part of a corresponding portion of the selectively openable opening, the feature selected from the group consisting of a door, a cover, a valve, a foil cover, a paper cover, an aperture size and/or configuration designed to control fluid flow, a dissolvable membrane, a selectively dissolvable membrane (e.g., a biopolymer), and any combinations thereof.

15. A system according to example 14, wherein the feature includes a foil cover removably covering the two or more separate reservoir chambers.

16. A system according to example 14, wherein the feature includes a foil cover removably covering each of the two or more separate reservoir chambers.

17. A system according to any one of the preceding examples, further comprising a holder cover designed and configured to be attached to the holder such that when the holder is attached to the holder cover a third space is formed between the holder cover and the holder with an opening to the third space positioned at at least a portion of a peripheral edge between the holder cover and the holder, the holder cover including: a fluid flow chamber having a size and configuration to hold a first volume of fluid from the first aquatic environment; a fluid flow inlet shaped and configured to promote fluid from the first aquatic environment to flow into the fluid flow chamber; a fluid flow outlet positioned in a wall of the fluid flow chamber adjacent to the third space and shaped and configured to promote fluid from within the fluid flow chamber to flow into the third space.

18. A system according to example 17, further comprising one or more posts extending from the holder cover and wherein the holder includes a corresponding first one or more structural recesses into which each of the one or more posts conformally seat when the holder is attached to the holder cover.

19. A system according to any one of examples 17 and 18, further comprising a first one or more magnets located proximate the holder cover and positioned to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion, the first and second one or more magnets configured to connect the holder and holder cover to the hub.

20. A system according to any one of examples 18 and 19, wherein the one or more posts are magnetic and are designed and configured to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion and to connect the holder and holder cover to the hub. 21. A system according to any one of examples 19 and 20, wherein the hub includes a corresponding second one or more structural recesses, each designed and configured to conformally fit an end of a corresponding one of the one or more posts.

22. A system according to one of examples 17 to 21, wherein each of the first one or more structural recesses includes a hole shaped and configured to have the one or more posts extend through the hole.

23. A system according to one of examples 17 to 22, wherein a hub is connected to a main body of the optical sensing portion and the optical sensing portion further comprises a mechanism for moving the hub with respect to the main body, wherein the first and second one or more magnets are configured to maintain connection of the holder and holder cover to the hub when the hub is moved with respect to the main body.

24. A system according to example 23, wherein the one or more posts are designed and configured to provide structural support to said connection of the holder and holder cover to the hub when the hub is moved with respect to the main body.

25. A system according to one of examples 17 to 24, wherein the holder cover includes a structural scoop associated with the fluid flow inlet, the structural scoop designed and configured to promote flow of fluid from the first aquatic environment into the flow chamber.

26. A system according to one of examples 17 to 24, wherein the holder cover includes a structural scoop associated with the fluid flow outlet, the structural scoop designed and configured to promote flow of fluid from inside the flow chamber to the first space.

27. A system according to example 17, wherein the holder cover is removably attachable to the holder.

28. A system according to example 17, wherein the holder cover is attached to the holder.

29. A chemical indicator apparatus for an aquatic environment analysis system, the aquatic environment analysis system including an optical sensing portion at least a portion of which is designed and configured to be in contact with a first aquatic environment, the optical sensing portion including a first optical reader, the chemical indicator apparatus comprising: a holder having one or more chemical indicators; a holder cover designed and configured to be attached to the holder such that when the holder is attached to the holder cover a first space is formed between the holder cover and the holder with an opening to the first space positioned at at least a portion of a peripheral edge between the holder cover and the holder, the holder cover including: a fluid flow chamber having a size and configuration to hold a first volume of fluid from the first aquatic environment; a fluid flow inlet shaped and configured to promote fluid from the first aquatic environment to flow into the fluid flow chamber; a fluid flow outlet positioned in a wall of the fluid flow chamber adjacent to the first space and shaped and configured to promote fluid from within the fluid flow chamber to flow into the first space.

30. A system according to example 29, further comprising a first one or more magnets located proximate the holder cover and positioned to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion, the first and second one or more magnets configured to connect the holder and holder cover to the hub.

31. A system according to example 29, further comprising one or more posts extending from the holder cover and wherein the holder includes a corresponding first one or more structural recesses into which each of the one or more posts conformally seat when the holder is attached to the holder cover.

32. A system according to example 31, further comprising a first one or more magnets located proximate the holder cover and positioned to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion, the first and second one or more magnets configured to connect the holder and holder cover to the hub.

33. A system according to example 32, wherein the one or more posts are magnetic and are designed and configured to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion and to connect the holder and holder cover to the hub.

34. A system according to example 33, wherein the hub includes a corresponding second one or more structural recesses, each designed and configured to conformally fit an end of a corresponding one of the one or more posts.

35. A system according to example 32, wherein each of the first one or more structural recesses includes a hole shaped and configured to have the one or more posts extend through the hole.

36. A system according to example 35, wherein a hub is connected to a main body of the optical sensing portion and the optical sensing portion further comprises a mechanism for moving the hub with respect to the main body, wherein the first and second one or more magnets are configured to maintain connection of the holder and holder cover to the hub when the hub is moved with respect to the main body. 37. A system according to example 36, wherein the one or more posts are designed and configured to provide structural support to said connection of the holder and holder cover to the hub when the hub is moved with respect to the main body.

38. A system according to any one of examples 29 to 37, wherein the holder cover includes a structural scoop associated with the fluid flow inlet, the structural scoop designed and configured to promote flow of fluid from the first aquatic environment into the flow chamber.

39. A system according to any one of examples 29 to 37, wherein the holder cover includes a structural scoop associated with the fluid flow outlet, the structural scoop designed and configured to promote flow of fluid from inside the flow chamber to the first space.

40. A system according to any one of examples 29 to 37, wherein the holder cover is removably attachable to the holder.

41 . A system according to any one of examples 29 to 37, wherein the holder cover is attached to the holder.

42. A system according to any one of examples 29 to 37, wherein each of the one or more chemical indicators has a first side reactive for indicating a level of a predetermined constituent of the first aquatic environment and a second side opposite the first side.

43. A system according to example 42, wherein the holder is positioned with respect to the optical sensing portion such that the second side is positioned facing the first optical reader and the first side is positioned facing in a direction away from the first optical reader

44. An aquatic environment analysis system comprising: an optical sensing portion, at least a portion of the optical sensing portion designed and configured to be in contact with a first aquatic environment and including a first optical reader; a holder having one or more chemical indicators, each of the one or more chemical indicators having a first side reactive for indicating a level of a predetermined constituent of the first aquatic environment and a second side opposite the first side, the holder being positioned with respect to the optical sensing portion such that the second side is positioned facing the first optical reader and the first side is positioned facing in a direction away from the first optical reader; a holder cover removably attachable to the holder such that when the holder is attached to the holder cover a first space is formed between the holder cover and the holder with an opening to the first space positioned at at least a portion of a peripheral edge between the holder cover and the holder, the holder cover including: a fluid flow chamber having a size and configuration to hold a first volume of fluid from the first aquatic environment; a fluid flow inlet shaped and configured to promote fluid from the first aquatic environment to flow into the fluid flow chamber; a fluid flow outlet positioned in a wall of the fluid flow chamber adjacent to the first space and shaped and configured to promote fluid from within the fluid flow chamber to flow into the first space.

45. A system according to example 44, further comprising one or more posts extending from the holder cover and wherein the holder includes a corresponding first one or more structural recesses into which each of the one or more posts conformally seat when the holder is attached to the holder cover.

46. A system according to one of examples 44 and 45, further comprising a first one or more magnets located proximate the holder cover and positioned to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion, the first and second one or more magnets configured to connect the holder and holder cover to the hub.

47. A system according to one of examples 45 and 46, wherein the one or more posts are magnetic and are designed and configured to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion and to connect the holder and holder cover to the hub.

48. A system according to any one of examples 46 and 47, wherein the hub includes a corresponding second one or more structural recesses, each designed and configured to conformally fit an end of a corresponding one of the one or more posts.

49. A system according to one of examples 45 to 48, wherein each of the first one or more structural recesses includes a hole shaped and configured to have the one or more posts extend through the hole.

50. A system according to one of examples 44 to 49, wherein a hub is connected to a main body of the optical sensing portion and the optical sensing portion further comprises a mechanism for moving the hub with respect to the main body, wherein the first and second one or more magnets are configured to maintain connection of the holder and holder cover to the hub when the hub is moved with respect to the main body.

51. A system according to example 50, wherein the one or more posts are designed and configured to provide structural support to said connection of the holder and holder cover to the hub when the hub is moved with respect to the main body. 52. A system according to one of examples 44 to 51, wherein the holder cover includes a structural scoop associated with the fluid flow inlet, the structural scoop designed and configured to promote flow of fluid from the first aquatic environment into the flow chamber.

53. A system according to one of examples 44 to 52, wherein the holder cover includes a structural scoop associated with the fluid flow outlet, the structural scoop designed and configured to promote flow of fluid from inside the flow chamber to the first space.

54. A system according to one of examples 44 to 53, wherein the second side is treated to not be reactive to the level of a predetermined constituent.

55. A system according to one of examples 44 to 54, wherein the holder includes a window proximate the second side, the window and/or the second side configured to: allow the optical reader to read one or more optical qualities of the at least one indicator through the window; and not allow the first aquatic environment to contact the at least one indicator through the window.

56. A system according to one of examples 44 to 55, wherein the holder includes a plurality of chemical indicators.

57. A system according to example 56, wherein two or more of the plurality of chemical indicators are included in a location of the holder such that the first side is at least partially enclosed by the first reservoir.

58. A system according to example 57, wherein the calibration solution is in contact with the two or more of the plurality of chemical indicators.

59. A system according to example 56, wherein a first reservoir of the holder includes two or more separate reservoir chambers.

60. A system according to example 59, wherein each of the two or more separate reservoir chambers includes one or more of the plurality of chemical indicators at least partially enclosed by the corresponding one of the two or more separate reservoir chambers.

61. A system according to any one of examples 59 and 60, wherein each of the two or more separate reservoir chambers includes a feature as part of a corresponding portion of a selectively openable opening of the holder, the feature selected from the group consisting of a door, a cover, a valve, a foil cover, a paper cover, an aperture size and/or configuration designed to control fluid flow, a dissolvable membrane, a selectively dissolvable membrane (e.g., a biopolymer), and any combinations thereof. 62. A system according to example 61, wherein the feature includes a foil cover removably covering the two or more separate reservoir chambers.

63. A system according to example 61, wherein the feature includes a foil cover removably covering each of the two or more separate reservoir chambers.

64. A system according to any one of the preceding examples, wherein the holder is removably attachable to the optical sensing portion.

65. A system according to any one of the preceding examples, wherein at least a portion of the optical sensing portion is configured to be submersed in the first aquatic environment.

66. A system according to any one of the preceding examples, wherein the aquatic environment includes an environment selected from the group consisting of an aquarium, a sump of an aquarium, a plumbing component of an aquarium, a swimming pool, a diving pool, a wave pool, a hot tub, a fish pond, a potable water supply, a sewage treatment infrastructure, a water treatment system, a water fountain, a water display, a lake, a lagoon, a food processing system, an aquaculture environment, a recirculating aquaculture system, a submerged oceanic aquaculture system, a river aquaculture system, an estuary aquaculture system, a fish transport system, an invertebrate transport system, an animal transport system, a supporting equipment component of any of the foregoing, and any combinations thereof.

67. A system according to example 66, wherein the supporting equipment component includes a component selected from the group consisting of a plumbing component, a heater, a filter, a skimmer, a control system, a holding tank, a display tank, a filtration canister, and any combinations thereof.

68. A system according to example 67, wherein the plumbing component includes a component selected from the group consisting of a sump, a pump, a pipe, a storage chamber, a valve, a refugium, a quarantine chamber, and any combinations thereof.

69. A system according to any one of the preceding examples, wherein the holder includes at least a portion of the holder having a shape selected from the group consisting of round, planar, discoidal, cylindrical, frusto-conical, spherical, ellipsoidal, parallelepiped, cuboid, and any combinations thereof.

70. A system according to any one of the preceding examples, wherein the holder includes at least portion of the holder having a discoidal shape.

71. A system according to any one of the preceding examples, wherein the holder includes: a first discoidal shaped portion having a first surface having an outer circumference; a pseudo- spherical wall extending from the circumference of the first discoidal shaped surface in a direction opposite the first surface; and a second space positioned opposite the first surface, the second space enclosed by the first discoidal shaped portion and the wall.

72. A system according to example 71, wherein a first reservoir of the holder is included in at least a portion of the second space.

73. A system according to any one of examples 71 and 72, wherein the first discoidal shaped portion includes a second surface opposite the first surface and a selectively openable opening of the holder is a circular opening formed by a terminal end of the pseudo- spherical wall and positioned opposite the second surface.

74. A system according to any one of the preceding examples, wherein a selectively openable opening of the holder includes a feature selected from the group consisting of a door, a cover, a valve, a foil cover, a film cover, a paper cover, an aperture size and/or configuration designed to control fluid flow, a dissolvable membrane, a selectively dissolvable membrane (e.g., a biopolymer), and any combinations thereof.

75. A system according to one of any one of the preceding examples, wherein a selectively openable opening of the holder is covered by a removable foil.

76. A system according to any one of the preceding examples, wherein a selectively openable opening of the holder is covered by a removable film.

77. A system according to any one of the preceding examples, wherein the holder includes a material selected from the group consisting of, a plastic, a polymer, an acrylic, a cyclic olefin copolymer (“COP”), a quartz, a glass, a polyethylene terephthalate (“PET”), and any combinations thereof.

78. A system according to any one of the preceding examples, wherein the holder includes a form selected from the group consisting of solid, fenestrated, trussed, stretched membrane, and any combinations thereof.

79. A system according to any one of the preceding examples, wherein the holder is formed of a monolithic material.

80. A system according to any one of the preceding examples, wherein the holder is constructed of two or more parts.

81. A system according to any one of the preceding examples, wherein the first optical reader includes a plurality of separate optical reading devices. 82. A system according to any one of the preceding examples, wherein the first optical reader includes a component selected from the group consisting of a light pipe, a lens, an optical sensor, a light source, one or more optical lenses, one or more light pipes, one or more sensors capable of detecting light and/or other energy from a chemical indicator, a light source capable of producing a light and/or other energy for delivery to a chemical indicator, and any combinations thereof.

83. A system according to any one of the preceding examples, wherein the one or more chemical indicators includes an indicator component selected from the group consisting of a dye, an immobilized dye, an immobilizing medium, an optical filtering film, an optical blocking film, and any combinations thereof.

84. A system according to example 83, wherein the immobilizing medium includes a medium selected from the group consisting of a gel, a hydrogel, a solgel, an aerogel, a chalcogel, a polymer matrix, a cellulosic matrix, and any combinations thereof.

85. A system according to any one of the preceding examples, wherein at least one of the one or more chemical indicators includes a dye covalently bonded to a cellulose fiber that is immobilized in a hydrogel.

86. A system according to any one of examples 83, 84, and 85, wherein the dye includes a dye selected from the group consisting of a calcium detecting aminonaphthalimide, a calcium detecting perylenediamide, a magnesium detecting dye based on a aminonaphthalimide, a magnesium detecting dye based on a photon induced electron transfer process (PET), a magnesium detecting dye based on a intramolecular charge transfer process (ICT), a magnesium detecting perylenediamide, a carbon dioxide sensitive dye based on a aminonaphthalimide, a carbon dioxide sensitive dye based on a photon induced electron transfer process (PET), a carbon dioxide sensitive dye based on a intramolecular charge transfer process (ICT), a carbon dioxide sensitive perylenediamide, and any combinations thereof.

87. A system according to any one of the preceding examples, wherein the one or more chemical indicators includes a chemical indicator reactive for indicating a level of a constituent selected from the group consisting of pH, hardness, calcium, magnesium, oxygen, dissolved oxygen, carbon dioxide, ammonia, phosphate, nitrate, potassium, nitrite, carbon, a molecular organism, a metabolite, a mineral, an inorganic matter, an organic matter, a living organism, a conductive material, a heavy metal, a pathogen, and any combinations thereof.

88. A system according to any one of the preceding examples, wherein the one or more chemical indicators includes an indicator that is reversible. 89. A system according to any one of the preceding examples, wherein the one or more chemical indicators includes an indicator that is reactive based on a process that includes fluorescence, fluorescence decay, phase fluorescence, electromagnetic energy absorptance, electromagnetic energy absorbance, change in electromagnetic energy absorptance, change in electromagnetic energy absorbance, electromagnetic energy reflectivity, change in electromagnetic energy reflectivity, color, change in color, change in refractive index, refractive index, conductivity, change in conductivity, and any combinations thereof.

90. A method of calibrating an aquatic environment analysis system comprising: locating at least a portion of a first optical reader and at least a portion of a chemical indicator holder having a first chemical indicator in a first aquatic environment; aligning the first optical reader with a first side of the first chemical indicator wherein a second side of the first chemical indicator is in contact with a calibration solution and is reactive for indicating a level of a predetermined constituent of the first aquatic environment; using the first optical reader, measuring a first calibration reading of the second side in contact with the calibration solution, the measuring of the first calibration reading occurring through a fluid of the first aquatic environment in a first space between the first side and the optical reader, wherein the second side is not reactive to the predetermined constituent in the fluid in the first space; allowing the first aquatic environment to contact the second side without the calibration solution; using the first optical reader, measuring through the first aquatic environment in the first space an optical quality of the first indicator while the second side is in contact with the first aquatic environment; and calibrating the optical quality with the first calibration reading to obtain a value of a level of the predetermined constituent in the first aquatic environment.

91. A method according to example 90, further comprising using the first optical reader and/or a second optical reader, measuring a second calibration reading of the fluid in the first space.

92. A method according to example 91, wherein the measuring of the second calibration reading is performed while the second side is in contact with the calibration solution.

93. A method according to example 91, wherein the measuring of the second calibration reading is performed while the second side is in contact with the first aquatic environment. 94. A method according to one of examples 91 to 93, wherein the calibrating the optical quality includes using the second calibration reading.

95. A method according to one of examples 90 to 94, wherein the locating includes submersing at least a portion of a first optical reader and at least a portion of a chemical indicator holder in the first aquatic environment.

96. A method according to one of examples 90 to 95, wherein said aligning includes connecting the holder to an optical sensing portion, wherein the first optical reader is part of the optical sensing portion.

97. A method according to example 96, wherein said connecting includes magnetically connecting.

98. A method according to any one of examples 96 and 97, wherein said connecting includes structurally connecting the holder to a hub of the optical sensing portion.

99. A method according to one of examples 96 to 98, wherein said aligning includes moving the holder with respect to the first optical reader after the holder is connected to the optical sensing portion.

100. A method according to one of examples 90 to 99, wherein the calibration solution is contained within a first reservoir of the holder.

101. A method according to example 100, wherein the reservoir includes a first removable cover and wherein said allowing includes removing the cover from the reservoir to remove the calibration solution and allow a fluid of the first aquatic environment to enter the reservoir.

102. A method according to example 101, further comprising: removing the holder from being in contact with the first aquatic environment prior to removing the cover; and replacing the holder to be in contact with the first aquatic environment to allow the fluid to contact the second side of the first chemical indicator.

103. A method according to example 102, wherein the calibration solution is removed from the reservoir while the holder is not in contact with the first aquatic environment.

104. A method according to one of examples 90 to 103, wherein the calibration solution is removed from being in contact with the second side while the holder is in contact with the first aquatic environment.

105. A method according to one of examples 90 to 101, further comprising removing the holder from being in contact with the first aquatic environment, wherein the calibration solution is removed from being in contact with the second side while the holder is not in contact with the first aquatic environment.

106. A method according to one of examples 90 to 105, further comprising communicating the first calibration value and/or a raw value of the optical quality to a processor, wherein the calibrating is performed by the processor.

107. A method according to example 106, wherein the processor is located in a location selected from the group consisting of within the optical sensing portion, connected to the optical sensing portion, in a computing device connected to the optical sensing portion, and any combinations thereof.

108. A method according to one of examples 90 to 107, further comprising providing the level of the predetermined constituent to a user of the analysis system via a display device.

109. A method of calibrating an aquatic environment analysis system, the aquatic environment analysis system including a first optical reader and a chemical indicator holder that is positioned opposite the first optical reader with a first space therebetween, the chemical indicator holder including at least one chemical indicator reactive for indicating a level of a predetermined constituent of a first aquatic environment, a first reservoir enclosing a first side of a first indicator of the at least one chemical indicators, and a calibration solution removably enclosed in the first reservoir and in contact with the first side, the method comprising: locating the first optical reader and at least a portion of the chemical indicator holder having the first indicator in the first aquatic environment; using the first optical reader, taking a first calibration reading of the first indicator while the first side is in contact with the calibration solution and a first portion of the first aquatic environment is in the first space; removing the calibration solution from the first reservoir; allowing a second portion of the first aquatic environment to contact the first side; using the first optical reader, measuring through the first aquatic environment in the first space an optical quality of the first indicator while the second portion is in contact with the first side; and calibrating the optical quality using the first calibration reading to obtain a value of a level of the predetermined constituent in the first aquatic environment.

110. A method according to example 109, further modified by one or more of examples 2 to 28 (where applicable), examples 45 to 87 (where applicable), and examples 91 to 108 (where applicable). 111. A method of calibrating an aquatic environment analysis system comprising: submersing a first optical reader and at least a portion of a chemical indicator holder having a first chemical indicator in a first aquatic environment; aligning the first optical reader with a first side of the first chemical indicator wherein a second side of the first chemical indicator is in contact with a calibration solution and is reactive for indicating a level of a predetermined constituent of the first aquatic environment; using the first optical reader, measuring a first calibration reading of the second side in contact with the calibration solution, the measuring of the first calibration reading occurring through a fluid of the first aquatic environment in a first space between the first side and the optical reader, wherein the second side is not reactive to the predetermined constituent in the fluid in the first space; using the first optical reader and/or a second optical reader, measuring a second calibration reading of the fluid in the first space while the second side is in contact with the calibration solution; allowing the first aquatic environment to contact the second side without the calibration solution; using the first optical reader, measuring through the first aquatic environment in the first space an optical quality of the first indicator while the second side is in contact with the first aquatic environment; calibrating the optical quality with the first and second calibration readings to obtain a value of a level of the predetermined constituent in the first aquatic environment.

112. A method according to example 111, further modified by one or more of examples 2 to 28 (where applicable), examples 45 to 87 (where applicable), and examples 91 to 108 (where applicable).

[0083] Machine/Computer Execution

[0084] It is to be noted that any one or more of the aspects, ideas, concepts, implementations, examples, and embodiments described herein (e.g., processing a calibration reading, processing a measurement of a level of a constituent in water, processing a calibration of a measurement, controlling a component of an aquatic environment analysis system, etc.) may be conveniently implemented using one or more machines (referred to herein as a computing device, e.g., one or more user computing devices, one or more server devices,) programmed according to the teachings of the present disclosure, as will be apparent to those of ordinary skill in the computer art. Appropriate software coding can readily be prepared by skilled programmers based on the teachings of the present disclosure, as will be apparent to those of ordinary skill in the software and related platform arts. Aspects, ideas, concepts, implementations, examples, and embodiments discussed herein may employ software and/or software modules and may also include appropriate hardware for assisting in the implementation of the machine executable instructions of the software and/or software module.

[0085] Such software may be a computer program product that employs a machine-readable storage medium. A machine-readable storage medium may be any medium that is capable of storing and/or encoding a sequence of instructions for execution by a machine (e.g., a computing device) and that causes the machine to perform any one of the methodologies and/or embodiments described herein, except that a machine-readable storage medium is not a medium that simply transports data from one point to another without a time period (however short or long) of storage in a hardware material (e.g., a signal is not a machine-readable storage medium). Examples of a machine-readable storage medium include, but are not limited to, a solid state memory, a flash memory, a random access memory (e.g., a static RAM “SRAM”, a dynamic RAM “DRAM”, etc.), , a magnetic memory (e g., a hard disk, a tape, a floppy disk, etc.), an optical memory (e.g., a compact disc (CD), a digital video disc (DVD), a Blu-ray disc (BD); a readable, writeable, and/or re-writable disc, etc.), a read only memory (ROM), a programmable read-only memory (PROM), a field programmable read-only memory (FPROM), a one-time programmable non-volatile memory (OTP NVM), an erasable programmable read-only memory (EPROM), an electrically erasable programmable read-only memory (EEPROM), and any combinations thereof, and any combinations thereof. Such examples are hardware storage media. If used herein, the term “non-transitory” in relation to a medium refers to a hardware storage medium that stores machine-readable instructions for a period of time, even if that time period is extremely small or temporary, and even if the storage of such instructions is fragile or reliant upon the provision of a continued electrical source. The examples of a machine- readable storage medium listed above in this paragraph are considered non-transitory. For example, machine executable instructions for an aquatic environment analysis system (or a portion of an aquatic environment analysis system) and/or related aspects and/or related methods and/or data may, in addition to one or more other machine-readable storage media, be stored in a RAM memory of a computing device (e.g., in RAM memory of a processing device, in combination of memory locations in a distributed network environment, etc.) for short periods of time and/or only while the computing device is powered on. In such a case, the RAM memory may be referred to, for example, as a non-transitory medium, A machine-readable medium, as used herein, is intended to include a single medium as well as a collection of physically separate media (localized and/or dispersed physically as in a distributed digital platform having a portion of machine-executable instructions on one or more server computers and/or one or more user devices), such as, for example, a collection of compact disks, one or more hard disk drives in combination with a computer memory, an array of RAM modules, etc. When used herein, the term “memory” includes examples that would include one or more machine-readable storage media. As used herein, the term “machine-readable storage medium” does not include a signal, carrier wave, or similar non-hardware based forms of encoding data.

[0086] Such software may also include information (e.g., data, instructions, etc.) carried as a data signal on a data carrier, such as a carrier wave. For example, machine-executable information may be included as a data-carrying signal embodied in a data carrier in which the signal encodes a sequence of instructions, or portion thereof, for execution by a machine (e.g., a computing device) and any related information (e.g., data structures and data) that causes the machine to perform any one of the methodologies and/or embodiments described herein. Such examples are not to be considered a machine-readable storage medium as that term is used herein. However, it is contemplated that such examples may be used in implementing one or more aspects, ideas, concepts, implementations, examples, and embodiments of the current disclosure if appropriate.

[0087] Machine-executable instructions may be disbursed across a plurality of computing devices (e.g., one or more user devices and one or more server computers) and connected via one or more networks.

[0088] A computing device is any machine that is capable of executing machine-executable instructions to perform one or more tasks. A processing device, such as processing device 150, may be a computing device, include a computing device, and/or be included as part of a computing device. Examples of a computing device include, but are not limited to, a tablet, an electronic book reading device, a workstation computer, a terminal computer, a server computer, a laptop computer, a mobile telephone (e.g., a smartphone), a portable and/or handheld computing device, a wearable computing device (e.g., a smart watch, a smart wearable eyeglass, a smart wearable headset, an augmented reality wearable device, etc.), a web appliance, a network router, a network switch, a network bridge, one or more application specific integrated circuits, an application specific programmable logic device, an application specific field programmable gate array, any machine capable of executing a sequence of instructions that specify an action to be taken by that machine (e g., an optical, chemical, biological, quantum and/or nanoengineered system and/or mechanism), and any combinations thereof. In one example, a computing device may include and/or be included in, a kiosk. In another example, a computing device is a smartphone. A computing device may include and/or be programed with specific machine-executable instructions (e.g., to perform one or more of the features, aspects, examples, or implementation of the current disclosure; to operate the computing device generally) and include required circuitry and components such that the combination of the circuitry/components and the instructions allow it to perform as a specialized machine in one or more of the implementations disclosed in the current disclosure. For example, a computing device may utilize any of a variety of known or yet to be developed operating systems, firmware, and/or other software for its operation. Examples of an operating system include, but are not limited to, Apple’s iOS, Amazon’s Fire OS, Google’s Android operating system, Microsoft’s Windows Phone operating system, Microsoft’s Windows operating system, Apple’s Operating System, a Linux-kernel based operating system, and any combinations thereof.

[0089] A network is a way for connecting multiple computing devices (and/or other devices such as, but not limited to, an aquatic environment analysis system or related apparatus, a display device) to each other for communicating information (e.g., data, machine-executable instructions, image files, video files, electronic messages, etc.). Examples of a network include, but are not limited to, a wide area network (e.g., the Internet, an enterprise network), a local area network (e.g., a network associated with an office, a building, a campus or other relatively small geographic space), a short distance network connection, a telephone network, a data network associated with a telephone/voice provider (e.g., a mobile communications provider data and/or voice network), another data network, a direct connection between two computing devices (e.g., a peer-to-peer connection), a proprietary service-provider network (e.g., a cable provider network), a wired connection, a wireless connection (e.g., a Bluetooth connection, a Wireless Fidelity (Wi-Fi) connection (such as an IEEE 802.11 connection), a Worldwide Interoperability for Microwave Access connection (WiMAX) (such as an IEEE 802.16 connection), a Global System for Mobile Communications connection (GSM), a Personal Communications Service (PCS) connection, a Code Division Multiplex Access connection (CDMA), and any combinations thereof. A network may employ one or more wired, one or more wireless, and/or one or more other modes of communication. A network may include any number of network segment types and/or network segments. In one example, a network connection between two computing devices may include a Wi-Fi connection between a sending computing device and a local router, an Internet Service Provider (ISP) owned network connecting the local router to the Internet, an Internet network (e.g., itself potentially having multiple network segments) connection connecting to one or more server computing devices and also to a wireless network (e.g., mobile phone) provider of a recipient computing device, and a telephone-service-provider network connecting the Internet to the recipient computing device.

[0090] FIG. 16 illustrates one example diagrammatic representation of one implementation of a computing device 1600. Computing device 1600 includes a processing element 1605, a memory 1610, a display generator 1615, a user input 1620, a networking element 1625, and a power supply 1630. Processing element 1605 includes circuitry and/or machine-executable instructions (e.g., in the form of firmware stored within a memory element included with and/or associated with processing element 1605) for executing instructions for completing one or more tasks (e.g., tasks associated with one or more of the implementations, methodologies, features, aspects, and/or examples described herein). Examples of a processing element include, but are not limited to, a microprocessor, a microcontroller, one or more circuit elements capable of executing a machineexecutable instruction, and any combinations thereof.

[0091] Memory 1610 may be any device capable of storing data (e.g., a calibration reading, a an optical reading from an optical reader, a calibration value, user data as part of or affiliated with an aquatic environment analysis system, information input by a user, information stored by a user, information captured by a sensor or other component of an aquatic environment analysis system, etc.), machine-executable instructions, an operating system, an “app” as part of an aquatic environment analysis system or associated processing device, a basic input/output system (BIOS) including basic routines that help to transfer information between components of a computing device, and/or other information related to one or more of the implementations, methodologies, features, aspects, and/or examples described herein. A memory, such as memory 1610, may include one or more machine-readable storage medium.

[0092] A memory may be removable from device 1600. A memory, such as memory 1610, may include and/or be associated with a memory access device. For example, a memory may include a medium for storage and an access device including one or more circuitry and/or other components for reading from and/or writing to the medium. In one such example, a computing device may include a port (e.g., a Universal Serial Bus (USB) port) for accepting a memory component (e.g., a removable flash USB memory device).

[0093] Device 1600 includes camera 1615 connected to processing element 1605 (and other components). Examples of a camera include, but are not limited to, a still image camera, a video camera, and any combinations thereof.

[0094] Display component 1620 is connected to processing element 1605 for providing a display according to any one or more of the implementations, examples, aspects, etc. of the current disclosure (e.g., providing an interface, displaying a captured image, displaying a converted simulated endoscopic image, etc.). A display component 1615 may include a display element, a driver circuitry, display adapter, a display generator, machine-executable instructions stored in a memory for execution by a processing element for displaying still and/or moving images on a screen, and/or other circuitry for generating one or more displayable images for display via a display element. Example display elements are discussed below. In one example, a display element is integrated with device 1600 (e.g., a built-in LCD touch screen). In another example, a display element is associated with device 1600 in a different fashion (e.g., an external LCD panel connected via a display adapter of display component 1615, a wearable headset having one or more display panels wirelessly connected to device 1600).

[0095] User input 1625 is configured to allow a user to input one or more commands, instructions, and/or other information to computing device 1600. For example, user input 1625 is connected to processing element 1605 (and optionally to other components directly or indirectly via processing element 1605) to allow a user to interface with computing device 1600 (e.g., to actuate camera 1615, to operate a component of an aquatic environment analysis system, to input instructions, information, or other inputs for performing (or as otherwise needed for) one or more aspects and/or methodologies of the present disclosure). Examples of a user input include, but are not limited to, a keyboard, a keypad, a screen displayable input (e.g., a screen displayable keyboard), a button, a toggle, a microphone (e.g., for receiving audio instructions), a pointing device, a joystick, a gamepad, a cursor control device (e.g., a mouse), a touchpad, an optical scanner, a video/image capture device (e.g., a camera), a touch screen of a display element/component, a pen device (e.g., a pen that interacts with a touch screen and/or a touchpad), a motion and/or image detecting device (e.g., a three dimensional motion detector) for receiving user gesture commands, and any combination thereof. It is noted that camera 1615 and/or a touch screen of a display element of display component 1620 may function also as an input element. It is also contemplated that one or more commands, data, and/or other information may be input to a computing device via a data transfer over a network and/or via a memory device (e.g., a removable memory device). A user input, such as user input 1625, may be connected to computing device 1600 via an external connector (e.g., an interface port).

[0096] External interface element 1630 includes circuitry and/or machine-executable instructions (e.g., in the form of firmware stored within a memory element included with and/or associated with interface element 1630) for communicating with one or more additional computing devices and/or connecting an external device to computing device 1600. An external interface element, such as element 1630, may include one or more external ports. In another example, an external interface element includes an antenna element for assisting with wireless communication. Examples of an external interface element include, but are not limited to, a network adapter, a Small Computer System Interface (SCSI), an advanced technology attachment interface (ATA), a serial ATA interface (SATA), an Industry Standard Architecture (ISA) interface, an extended ISA interface, a Peripheral Component Interface (PCI), a Universal Serial Bus (USB), an IEEE 1394 interface (FIREWIRE), and any combinations thereof. A network adapter includes circuitry and/or machine-executable instructions configured to connect a computing device, such as computing device 1600, to a network.

[0097] Power supply 1630 is shown connected to other components of computing device 1605 to provide power for operation of each component. Examples of a power supply include, but are not limited to, an internal power supply, an external power supply, a battery, a fuel cell, a connection to an alternating current power supply (e.g., a wall outlet, a power adapter, etc.), a connection to a direct current power supply (e.g., a wall outlet, a power adapter, etc.), and any combinations thereof.

[0098] Components of device 1600 (processing element 1605, memory 1610, camera 1615, display component 1620, user input 1625, interface element 1630, power supply 1635) are shown as single components. A computing device may include multiple components of the same type. A function of any one component may be performed by any number of the same components and/or in conjunction with another component. For example, it is contemplated that the functionality of any two or more of processing element 1605, memory 1610, camera 1615, display component 1620, user input 1625, interface element 1630, power supply 1635, and another component of a computing device may be combined in an integrated circuit. In one such example, a processor (e.g., processing element 1605) may include a memory for storing one or more machine executable instructions for performing one or more aspects and/or methodologies of the present disclosure. Functionality of any one or more components may also be distributed across multiple computing devices. Such distribution may be in different geographic locations (e.g., connected via a network). Components of device 1600 are shown as internal components to device 1600. A component of a computing device, such as device 1600, may be associated with the computing device in a way other than by being internally connected.

[0099] Components of computing device 1600 are shown connected to other components. Examples of ways to connect components of a computing device include, but are not limited to, a bus, a component connection interface, another type of connection, and/or any combinations thereof. Examples of a bus and/or component connection interface include, but are not limited to, a memory bus, a memory controller, a peripheral bus, a local bus, a parallel bus, a serial bus, a SCSI interface, an ATA interface, an SATA interface, an ISA interface, a PCI interface, a USB interface, a FIREWIRE interface, and any combinations thereof. Various bus architectures are known. Select connections and components in device 1600 are shown. For clarity, other connections and various other well-known components (e.g., an audio speaker, a printer, etc.) have been omitted and may be included in a computing device. Additionally, a computing device may omit in certain implementations one or more of the shown components.

[00100] As discussed above, one example of a computing device that may be utilized in one or more of the implementations of a method of the present disclosure is a handheld computing device. FIG. 17 illustrates one example of a portable handheld computing device in the form of a smartphone 1700. Smartphone 1700 includes a body 1705, a microphone input element 1710, a display element 1715, and a speaker output element 1720. Display element 1715 may be a touch screen to provide a user with additional input interface capabilities. A computing device, such as smartphone 1700, may be used in a variety of ways with respect to any of the implementations, embodiments, and/or methodologies described herein. Exemplary ways to utilize smartphone 1700 (or another computing device) include, but are not limited to, receiving an instruction (and/or other input, request, etc.) from a user of a computing device, presenting information or other displayable aspects to a user of a digital platform, and any combinations thereof. [00101] Examples of a display element or component include, but are not limited to, a computer monitor, a liquid crystal display (LCD) display screen, a light emitting diode (LED) display screen, a touch display, a cathode ray tube (CRT), a plasma display, a projection device, a holographic image projection device, and any combinations thereof. A display element may include, be connected with, and/or associated with adjunct elements to assist with the display of still and/or moving images. Examples of an adjunct display elements include, but are not limited to, a display generator (e.g., image/image display circuitry), a display adapter, a display driver, machine-executable instructions stored in a memory for execution by a processing element for displaying still and/or moving images on a screen, and any combinations thereof. Such display components may be included in any of a variety of known forms including, but not limited to, a display element directly connected to a computing device, a display element connected to a computing device via a wire, a display element wirelessly connected, a display element of a headset device, a display element of a stand-alone device, a display element of an eyeglass device, and any combinations thereof. In one example, a display element may be included in a display device, be a display device, and any combinations thereof.

[00102] In one exemplary aspect, an aquatic environment analysis system or related apparatus or method of the current disclosure includes one or more user interfaces that display images, text, and/or graphic elements to a user (e.g., via a display device associated with a user computing device) and allow a user to interact with an aquatic environment analysis system or related apparatus or method of the current disclosure (e.g., via one or more user inputs, such as a mouse, touch screen, etc.). For example, via one or more user interfaces, one or more users can access and interact with exemplary implementations of an aquatic environment analysis system or related apparatus or method of the current disclosure, enter one or more settings, view a display of a level of a constituent of an aquatic environment, make other interactions, and any combinations thereof.

[00103] A user may interact with a user interface via actuation of user interface elements and inputting information. Example input elements for interacting with a user include, but are not limited to, a radio button, a toggle switch, a pull-down menu, a text entry field, a hover button, a drag and drop functionality, a pop-up menu, a right-click menu, a screen displayed keyboard (e.g., a touchscreen keyboard), and any combinations thereof. Examples of interactions with a user interface include, but are not limited to, providing instructions to an aquatic environment analysis system or related apparatus, inputting information to an aquatic environment analysis system or related apparatus (e.g., information required for a user setting, information for a request, etc.), actuating a user interface element, making a selection (e.g., selecting an option in a menu), make an association of one object with another object, inputting text, typing (e.g., on an on-screen keyboard), hovering, gesturing (e.g., moving a hand or other object for detection by a motion detection device of a computing device), swiping in a direction of a user interface, and any combinations thereof. Other example user input element actuations and combinations of actuations will be understood and applicable depending on the particular computing device, interface, display element, etc.

[00104] A computer system, such as system 1600 and/or 1700, may include a positioning device (not shown) to determine the location of the computer system (and, optionally, a user of the computer system). Example positioning devices, such as a GPS (Global Positioning System) device, a GLONASS positioning system device, a Galileo positioning system device, another satellite based positioning device, a radio frequency based positioning system device, a Wi-Fi based positioning system device, a mobile network positioning system device, a local positioning system device, and any combinations thereof. A computer system, such as system 1600 and/or 1700, may also include an orientation device (not shown) capable of determining an orientation of the computer system in two or more dimensions. Orientation determining devices and related circuitries are well known to those of ordinary skill.

[00105] General

[00106] One or more systems and/or methods of the current disclosure may provide an ability to take one or more continuous readings of the presence of a constituent in an aquatic environment (e.g., readings that are frequent in time (e g., every predetermined set of minutes, such as every 15 minutes) and/or over a period of time, for example, without the need for resetting or replacing a chemical indicator). In one such example, such readings may occur for a plurality of constituents using a plurality of chemical indicators as part of a holder of the current disclosure. Information regarding one or more constituents can be provided to a user of a system and/or method of the current disclosure (e.g., via a display of processed information obtained from an optical element of a system of the current disclosure from one or more chemical indicators of a holder and processed to useable information, such as by a processing element as discussed above, for provision (such as via a display device) to the user).

[00107] Some of the details, concepts, aspects, features, characteristics, examples, and/or alternatives of a component/element discussed above with respect to one implementation, embodiment, and/or methodology may be applicable to a like component in another implementation, embodiment, and/or methodology, even though for the sake of brevity it may not have been repeated above. It is noted that any suitable combinations of components and elements of different implementations, embodiments, and/or methodologies (as well as other variations and modifications) are possible in light of the teachings herein, will be apparent to those of ordinary skill, and should be considered as part of the spirit and scope of the present disclosure. Additionally, functionality described with respect to a single component/element is contemplated to be performed by a plurality of like components/elements (e.g., in a more dispersed fashion locally and/or remotely). Functionality described with respect to multiple components/elements may be performed by fewer like or different components/elements (e.g., in a more integrated fashion).

[00108] For example, for the sake of brevity, some of the details, concepts, examples, aspects, features, characteristics, and/or alternatives discussed with respect to one implementation, methodology, and/or embodiment of the current disclosure may not be repeated in a discussion of another implementation, methodology, and/or embodiment where such details, concepts, examples, aspects, features, characteristics, and/or alternatives may be applicable for like items or in combination with other items discussed with respect a different discussion herein and, as applicable, may be included in (or otherwise apply similarly with) the implementation of that additional discussion, except where noted or inapplicable/incompatible.

[00109] While details, concepts, aspects, features, characteristics, examples, and/or alternatives of various implementations and embodiments herein are described in the context of one or more embodiments or examples of an aquatic environment analysis system or related apparatus or method, it is contemplated that any such details, concepts, aspects, features, characteristics, examples, and/or alternatives and corresponding implementations and embodiments may also be utilized with a different embodiments or examples of an aquatic environment analysis system or related apparatus or method as applicable.

[00110] If present in a claim or a description herein, the use of ordinal terms (e.g., “first,” “second,” “third,” etc.) to modify another term or phrase, such use is solely as labels to distinguish one item from another item of the same name and should not itself be construed to impart any order, precedence, or priority of one item over another. If any method is presented herein through the use of a flowchart or other flow diagram with a sequential nature, it is noted that it may be possible for one or more of the steps depicted to be performed in a parallel fashion to each other. Additionally, methods presented in a particular order of steps may have their steps performed in a different order than presented as applicable as well as having fewer steps or additional steps involved in the method. As used herein, a “set” of items may include one or more of such items.

[00111] Exemplary embodiments have been disclosed above and illustrated in the accompanying drawings. It will be understood by those skilled in the art that various changes, omissions and additions may be made to that which is specifically disclosed herein without departing from the spirit and scope of the present invention.

Claims

What is claimed:

2. A system according to claim 1, wherein the second side is treated to not be reactive to the level of a predetermined constituent.

3. A system according to claim 1, wherein the holder includes a window proximate the second side, the window and/or the second side configured to: allow the optical reader to read one or more optical qualities of the at least one indicator through the window; and not allow the first aquatic environment to contact the at least one indicator through the window.

4. A system according to claim 1, wherein the holder is removably attachable to the optical sensing portion.

5. A system according to claim 1, wherein at least a portion of the optical sensing portion is configured to be submersed in the first aquatic environment.

6. A system according to claim 1, wherein the first optical reader is configured to take a second calibration reading of the portion of the first aquatic environment in the first space.

7. A system according to claim 4, wherein the holder includes one or more optical calibration reference locations and the first optical reader is configured to take the second calibration reading using at least one of the one or more optical calibration reference locations.

8. A system according to claim 5, wherein the one or more optical calibration reference locations includes a patch selected from the group consisting of a black reference patch, a white reference patch, a fluorescence reference patch, and any combinations thereof.

9. A system according to any one of claims 1 to 8, wherein the first optical reader is configured to take a second calibration reading of the portion of the first aquatic environment in the first space while the calibration solution is positioned inside the first reservoir.

10. A system according to any one of claims 1 to 8, wherein the aquatic environment includes an environment selected from the group consisting of an aquarium, a sump of an aquarium, a plumbing component of an aquarium, a swimming pool, a diving pool, a wave pool, a hot tub, a fish pond, a potable water supply, a sewage treatment infrastructure, a water treatment system, a water fountain, a water display, a lake, a lagoon, a food processing system, an aquaculture environment, a recirculating aquaculture system, a submerged oceanic aquaculture system, a river aquaculture system, an estuary aquaculture system, a fish transport system, an invertebrate transport system, an animal transport system, a supporting equipment component of any of the foregoing, and any combinations thereof.

11. A system according to claim 10, wherein the supporting equipment component includes a component selected from the group consisting of a plumbing component, a heater, a filter, a skimmer, a control system, a holding tank, a display tank, a filtration canister, and any combinations thereof.

12. A system according to claim 11, wherein the plumbing component includes a component selected from the group consisting of a sump, a pump, a pipe, a storage chamber, a valve, a refugium, a quarantine chamber, and any combinations thereof.

13. A system according to any one of the preceding claims, wherein the first space is exposed to a fluid of a plumbing component and/or control system of the aquatic environment.

14. A system according to any one of claims 1 to 8, wherein the holder includes at least a portion of the holder having a shape selected from the group consisting of round, planar, discoidal, cylindrical, frusto-conical, spherical, ellipsoidal, parallelepiped, cuboid, and any combinations thereof.

15. A system according to any one of claims 1 to 8, wherein the holder includes at least portion of the holder having a discoidal shape.

16. A system according to any one of claims 1 to 8, wherein the holder includes: a first discoidal shaped portion having a first surface having an outer circumference; a pseudo- spherical wall extending from the circumference of the first discoidal shaped surface in a direction opposite the first surface; and a second space positioned opposite the first surface, the second space enclosed by the first discoidal shaped portion and the wall.

17. A system according to claim 16, wherein the first reservoir is included in at least a portion of the second space.

18. A system according to claim 16, wherein the first discoidal shaped portion includes a second surface opposite the first surface and the selectively openable opening is a circular opening formed by a terminal end of the pseudo-spherical wall and positioned opposite the second surface.

19. A system according to any one of claims 1 to 8, wherein the selectively openable opening includes a feature selected from the group consisting of a door, a cover, a valve, a foil cover, a film cover, a paper cover, an aperture size and/or configuration designed to control fluid flow, a dissolvable membrane, a selectively dissolvable membrane (e.g., a biopolymer), and any combinations thereof.

20. A system according to any one of claims 1 to 8, wherein the selectively openable opening is covered by a removable foil.

21. A system according to any one of claims 1 to 8, wherein the selectively openable opening is covered by a removable film.

22. A system according to claim 1, wherein the holder includes a material selected from the group consisting of, a plastic, a polymer, an acrylic, a cyclic olefin copolymer (“COP”), a quartz, a glass, a polyethylene terephthalate (“PET”), and any combinations thereof.

23. A system according to claim 1, wherein the holder includes a form selected from the group consisting of solid, fenestrated, trussed, stretched membrane, and any combinations thereof.

24. A system according to claim 1, wherein the holder is formed of a monolithic material.

25. A system according to any one of claims 1, 22, 23, and 24, wherein the holder is constructed of two or more parts.

26. A system according to any one of claims 1 to 8wherein the holder includes a plurality of chemical indicators.

27. A system according to claim 9, wherein two or more of the plurality of chemical indicators are included in a location of the holder such that the first side is at least partially enclosed by the first reservoir.

28. A system according to claim 10, wherein the calibration solution is in contact with the two or more of the plurality of chemical indicators.

29. A system according to claim 9, wherein the first reservoir includes two or more separate reservoir chambers.

30. A system according to claim 12, wherein each of the two or more separate reservoir chambers includes one or more of the plurality of chemical indicators at least partially enclosed by the corresponding one of the two or more separate reservoir chambers.

31. A system according to any one of claims 29 and 13, wherein each of the two or more separate reservoir chambers includes a feature as part of a corresponding portion of the selectively openable opening, the feature selected from the group consisting of a door, a cover, a valve, a foil cover, a paper cover, an aperture size and/or configuration designed to control fluid flow, a dissolvable membrane, a selectively dissolvable membrane (e.g., a biopolymer), and any combinations thereof.

32. A system according to claim 14, wherein the feature includes a foil cover removably covering the two or more separate reservoir chambers.

33. A system according to claim 14, wherein the feature includes a foil cover removably covering each of the two or more separate reservoir chambers.

34. A system according to claim 1, wherein the first optical reader includes a plurality of separate optical reading devices.

35. A system according to any one of claims 1 and 34, wherein the first optical reader includes a component selected from the group consisting of a light pipe, a lens, an optical sensor, a light source, one or more optical lenses, one or more light pipes, one or more sensors capable of detecting light and/or other energy from a chemical indicator, a light source capable of producing a light and/or other energy for delivery to a chemical indicator, and any combinations thereof.

36. A system according to claim 1, wherein the one or more chemical indicators includes an indicator component selected from the group consisting of a dye, an immobilized dye, an immobilizing medium, an optical filtering film, an optical blocking film, and any combinations thereof.

37. A system according claim 36, wherein the dye includes a dye selected from the group consisting of a calcium detecting aminonaphthalimide, a calcium detecting perylenediamide, a magnesium detecting dye based on a aminonaphthalimide, a magnesium detecting dye based on a photon induced electron transfer process (PET), a magnesium detecting dye based on a intramolecular charge transfer process (ICT), a magnesium detecting perylenediamide, a carbon dioxide sensitive dye based on a aminonaphthalimide, a carbon dioxide sensitive dye based on a photon induced electron transfer process (PET), a carbon dioxide sensitive dye based on a intramolecular charge transfer process (ICT), a carbon dioxide sensitive perylenediamide, and any combinations thereof.

38. A system according to claim 36, wherein the immobilizing medium includes a medium selected from the group consisting of a gel, a hydrogel, a solgel, an aerogel, a chalcogel, a polymer matrix, a cellulosic matrix, and any combinations thereof.

39. A system according to claim 38, wherein the dye includes a dye selected from the group consisting of a calcium detecting aminonaphthalimide, a calcium detecting perylenediamide, a magnesium detecting dye based on a aminonaphthalimide, a magnesium detecting dye based on a photon induced electron transfer process (PET), a magnesium detecting dye based on a intramolecular charge transfer process (ICT), a magnesium detecting perylenediamide, a carbon dioxide sensitive dye based on a aminonaphthalimide, a carbon dioxide sensitive dye based on a photon induced electron transfer process (PET), a carbon dioxide sensitive dye based on a intramolecular charge transfer process (ICT), a carbon dioxide sensitive perylenediamide, and any combinations thereof.

40. A system according to claim 1, wherein at least one of the one or more chemical indicators includes a dye covalently bonded to a cellulose fiber that is immobilized in a hydrogel.

41. A system according to claim 40, wherein the dye includes a dye selected from the group consisting of a calcium detecting aminonaphthalimide, a calcium detecting perylenediamide, a magnesium detecting dye based on a aminonaphthalimide, a magnesium detecting dye based on a photon induced electron transfer process (PET), a magnesium detecting dye based on a intramolecular charge transfer process (ICT), a magnesium detecting perylenediamide, a carbon dioxide sensitive dye based on a aminonaphthalimide, a carbon dioxide sensitive dye based on a photon induced electron transfer process (PET), a carbon dioxide sensitive dye based on a intramolecular charge transfer process (ICT), a carbon dioxide sensitive perylenediamide, and any combinations thereof.

42. A system according to any one of claims 1, 36, 38, and 40, wherein the one or more chemical indicators includes a chemical indicator reactive for indicating a level of a constituent selected from the group consisting of pH, hardness, calcium, magnesium, oxygen, dissolved oxygen, carbon dioxide, ammonia, phosphate, nitrate, potassium, nitrite, carbon, a molecular organism, a metabolite, a mineral, an inorganic matter, an organic matter, a living organism, a conductive material, a heavy metal, a pathogen, and any combinations thereof.

43. A system according to any one of claims 1, 36, 38, and 40, wherein the one or more chemical indicators includes an indicator that is reversible.

44. A system according to any one of claims 1, 36, 38, and 40, wherein the one or more chemical indicators includes an indicator that is reactive based on a process that includes fluorescence, fluorescence decay, phase fluorescence, electromagnetic energy absorptance, electromagnetic energy absorbance, change in electromagnetic energy absorptance, change in electromagnetic energy absorbance, electromagnetic energy reflectivity, change in electromagnetic energy reflectivity, color, change in color, change in refractive index, refractive index, conductivity, change in conductivity, and any combinations thereof.

45. A system according to any one of claims 1 to 8, further comprising a holder cover designed and configured to be attached to the holder such that when the holder is attached to the holder cover a third space is formed between the holder cover and the holder with an opening to the third space positioned at at least a portion of a peripheral edge between the holder cover and the holder, the holder cover including: a fluid flow chamber having a size and configuration to hold a first volume of fluid from the first aquatic environment; a fluid flow inlet shaped and configured to promote fluid from the first aquatic environment to flow into the fluid flow chamber; a fluid flow outlet positioned in a wall of the fluid flow chamber adjacent to the third space and shaped and configured to promote fluid from within the fluid flow chamber to flow into the third space.

46. A system according to claim 17, further comprising a first one or more magnets located proximate the holder cover and positioned to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion, the first and second one or more magnets configured to connect the holder and holder cover to the hub.

47. A system according to claim 17, further comprising one or more posts extending from the holder cover and wherein the holder includes a corresponding first one or more structural recesses into which each of the one or more posts conformally seat when the holder is attached to the holder cover.

48. A system according to claim 47, further comprising a first one or more magnets located proximate the holder cover and positioned to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion, the first and second one or more magnets configured to connect the holder and holder cover to the hub.

49. A system according to claim 47, wherein the one or more posts are magnetic and are designed and configured to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion and to connect the holder and holder cover to the hub.

50. A system according to claim 48, wherein the hub includes a corresponding second one or more structural recesses, each designed and configured to conformally fit an end of a corresponding one of the one or more posts.

51. A system according to claim 47, wherein each of the first one or more structural recesses includes a hole shaped and configured to have the one or more posts extend through the hole.

52. A system according to claim 49, wherein a hub is connected to a main body of the optical sensing portion and the optical sensing portion further comprises a mechanism for moving the hub with respect to the main body, wherein the first and second one or more magnets are configured to maintain connection of the holder and holder cover to the hub when the hub is moved with respect to the main body.

53. A system according to claim 52, wherein the one or more posts are designed and configured to provide structural support to said connection of the holder and holder cover to the hub when the hub is moved with respect to the main body.

54. A system according to claim 17, wherein the holder cover includes a structural scoop associated with the fluid flow inlet, the structural scoop designed and configured to promote flow of fluid from the first aquatic environment into the flow chamber.

55. A system according to claim 17, wherein the holder cover includes a structural scoop associated with the fluid flow outlet, the structural scoop designed and configured to promote flow of fluid from inside the flow chamber to the first space.

56. A system according to claim 17, wherein the holder cover is removably attachable to the holder.

57. A system according to claim 17, wherein the holder cover is attached to the holder.

58. A chemical indicator apparatus for an aquatic environment analysis system, the aquatic environment analysis system including an optical sensing portion at least a portion of which is designed and configured to be in contact with a first aquatic environment, the optical sensing portion including a first optical reader, the chemical indicator apparatus comprising: a holder having one or more chemical indicators; a holder cover designed and configured to be attached to the holder such that when the holder is attached to the holder cover a first space is formed between the holder cover and the holder with an opening to the first space positioned at at least a portion of a peripheral edge between the holder cover and the holder, the holder cover including: a fluid flow chamber having a size and configuration to hold a first volume of fluid from the first aquatic environment; a fluid flow inlet shaped and configured to promote fluid from the first aquatic environment to flow into the fluid flow chamber; a fluid flow outlet positioned in a wall of the fluid flow chamber adjacent to the first space and shaped and configured to promote fluid from within the fluid flow chamber to flow into the first space.

59. A system according to claim 29, further comprising a first one or more magnets located proximate the holder cover and positioned to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion, the first and second one or more magnets configured to connect the holder and holder cover to the hub.

60. A system according to claim 29, further comprising one or more posts extending from the holder cover and wherein the holder includes a corresponding first one or more structural recesses into which each of the one or more posts conformally seat when the holder is attached to the holder cover.

61. A system according to claim 31, further comprising a first one or more magnets located proximate the holder cover and positioned to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion, the first and second one or more magnets configured to connect the holder and holder cover to the hub.

62. A system according to claim 32, wherein the one or more posts are magnetic and are designed and configured to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion and to connect the holder and holder cover to the hub.

63. A system according to claim 33, wherein the hub includes a corresponding second one or more structural recesses, each designed and configured to conformally fit an end of a corresponding one of the one or more posts.

64. A system according to claim 32, wherein each of the first one or more structural recesses includes a hole shaped and configured to have the one or more posts extend through the hole.

65. A system according to claim 35, wherein a hub is connected to a main body of the optical sensing portion and the optical sensing portion further comprises a mechanism for moving the hub with respect to the main body, wherein the first and second one or more magnets are configured to maintain connection of the holder and holder cover to the hub when the hub is moved with respect to the main body.

66. A system according to claim 36, wherein the one or more posts are designed and configured to provide structural support to said connection of the holder and holder cover to the hub when the hub is moved with respect to the main body.

67. A system according to any one of claims 29 to 37, wherein the holder cover includes a structural scoop associated with the fluid flow inlet, the structural scoop designed and configured to promote flow of fluid from the first aquatic environment into the flow chamber.

68. A system according to any one of claims 29 to 37, wherein the holder cover includes a structural scoop associated with the fluid flow outlet, the structural scoop designed and configured to promote flow of fluid from inside the flow chamber to the first space.

69. A system according to any one of claims 29 to 37, wherein the holder cover is removably attachable to the holder.

70. A system according to any one of claims 29 to 37, wherein the holder cover is attached to the holder.

71. A system according to any one of claims 29 to 37, wherein each of the one or more chemical indicators has a first side reactive for indicating a level of a predetermined constituent of the first aquatic environment and a second side opposite the first side.

72. A system according to claim 42, wherein the holder is positioned with respect to the optical sensing portion such that the second side is positioned facing the first optical reader and the first side is positioned facing in a direction away from the first optical reader

73. An aquatic environment analysis system comprising: an optical sensing portion, at least a portion of the optical sensing portion designed and configured to be in contact with a first aquatic environment and including a first optical reader; a holder having one or more chemical indicators, each of the one or more chemical indicators having a first side reactive for indicating a level of a predetermined constituent of the first aquatic environment and a second side opposite the first side, the holder being positioned with respect to the optical sensing portion such that the second side is positioned facing the first optical reader and the first side is positioned facing in a direction away from the first optical reader; a holder cover removably attachable to the holder such that when the holder is attached to the holder cover a first space is formed between the holder cover and the holder with an opening to the first space positioned at at least a portion of a peripheral edge between the holder cover and the holder, the holder cover including: a fluid flow chamber having a size and configuration to hold a first volume of fluid from the first aquatic environment; a fluid flow inlet shaped and configured to promote fluid from the first aquatic environment to flow into the fluid flow chamber; a fluid flow outlet positioned in a wall of the fluid flow chamber adjacent to the first space and shaped and configured to promote fluid from within the fluid flow chamber to flow into the first space.

74. A system according to claim 44, further comprising one or more posts extending from the holder cover and wherein the holder includes a corresponding first one or more structural recesses into which each of the one or more posts conformally seat when the holder is attached to the holder cover.

75. A system according to claim 44, further comprising a first one or more magnets located proximate the holder cover and positioned to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion, the first and second one or more magnets configured to connect the holder and holder cover to the hub.

76. A system according to claim 46, wherein the one or more posts are magnetic and are designed and configured to magnetically connect to a second one or more magnets located in a hub of the optical sensing portion and to connect the holder and holder cover to the hub.

77. A system according to claim 46, wherein the hub includes a corresponding second one or more structural recesses, each designed and configured to conformally fit an end of a corresponding one of the one or more posts.

78. A system according to claim 45, wherein each of the first one or more structural recesses includes a hole shaped and configured to have the one or more posts extend through the hole.

79. A system according to claim 46, wherein a hub is connected to a main body of the optical sensing portion and the optical sensing portion further comprises a mechanism for moving the hub with respect to the main body, wherein the first and second one or more magnets are configured to maintain connection of the holder and holder cover to the hub when the hub is moved with respect to the main body.

80. A system according to claim 50, wherein the one or more posts are designed and configured to provide structural support to said connection of the holder and holder cover to the hub when the hub is moved with respect to the main body.

81. A system according to claim 44, wherein the holder cover includes a structural scoop associated with the fluid flow inlet, the structural scoop designed and configured to promote flow of fluid from the first aquatic environment into the flow chamber.

82. A system according to one of claims 44 to 81, wherein the holder cover includes a structural scoop associated with the fluid flow outlet, the structural scoop designed and configured to promote flow of fluid from inside the flow chamber to the first space.

83. A system according to one of claims 44 to 81, wherein the second side is treated to not be reactive to the level of a predetermined constituent.

84. A system according to one of claims 44 to 81, wherein the holder includes a window proximate the second side, the window and/or the second side configured to: allow the optical reader to read one or more optical qualities of the at least one indicator through the window; and not allow the first aquatic environment to contact the at least one indicator through the window.

85. A system according to one of claims 44 to 81, wherein the holder is removably attachable to the optical sensing portion.

86. A system according to one of claims 44 to 81, wherein at least a portion of the optical sensing portion is configured to be submersed in the first aquatic environment.

87. A system according to one of claims 44 to 81, wherein the aquatic environment includes an environment selected from the group consisting of an aquarium, a sump of an aquarium, a plumbing component of an aquarium, a swimming pool, a diving pool, a wave pool, a hot tub, a fish pond, a potable water supply, a sewage treatment infrastructure, a water treatment system, a water fountain, a water display, a lake, a lagoon, a food processing system, an aquaculture environment, a recirculating aquaculture system, a submerged oceanic aquaculture system, a river aquaculture system, an estuary aquaculture system, a fish transport system, an invertebrate transport system, an animal transport system, a supporting equipment component of any of the foregoing, and any combinations thereof.

88. A system according to claim 87, wherein the supporting equipment component includes a component selected from the group consisting of a plumbing component, a heater, a filter, a skimmer, a control system, a holding tank, a display tank, a filtration canister, and any combinations thereof.

89. A system according to claim 88, wherein the plumbing component includes a component selected from the group consisting of a sump, a pump, a pipe, a storage chamber, a valve, a refugium, a quarantine chamber, and any combinations thereof.

90. A system according to one of claims 44 to 81, wherein the holder includes at least a portion of the holder having a shape selected from the group consisting of round, planar, discoidal, cylindrical, frusto-conical, spherical, ellipsoidal, parallelepiped, cuboid, and any combinations thereof.

91. A system according to one of claims 44 to 81, wherein the holder includes at least portion of the holder having a discoidal shape.

92. A system according to one of claims 44 to 81, wherein the holder includes: a first discoidal shaped portion having a first surface having an outer circumference; a pseudo- spherical wall extending from the circumference of the first discoidal shaped surface in a direction opposite the first surface; and a second space positioned opposite the first surface, the second space enclosed by the first discoidal shaped portion and the wall.

93. A system according to claim 92, wherein a first reservoir of the holder is included in at least a portion of the second space.

94. A system according to claim 92, wherein the first discoidal shaped portion includes a second surface opposite the first surface and a selectively openable opening of the holder is a circular opening formed by a terminal end of the pseudo-spherical wall and positioned opposite the second surface.

95. A system according to one of claims 44 to 81, wherein a selectively openable opening of the holder includes a feature selected from the group consisting of a door, a cover, a valve, a foil cover, a film cover, a paper cover, an aperture size and/or configuration designed to control fluid flow, a dissolvable membrane, a selectively dissolvable membrane (e.g., a biopolymer), and any combinations thereof.

96. A system according to one of claims 44 to 81, wherein a selectively openable opening of the holder is covered by a removable foil.

97. A system according to one of claims 44 to 81, wherein a selectively openable opening is covered by a removable film.

98. A system according to one of claims 44 to 81, wherein the holder includes a material selected from the group consisting of, a plastic, a polymer, an acrylic, a cyclic olefin copolymer (“COP”), a quartz, a glass, a polyethylene terephthalate (“PET”), and any combinations thereof.

99. A system according to one of claims 44 to 81, wherein the holder includes a form selected from the group consisting of solid, fenestrated, trussed, stretched membrane, and any combinations thereof.

100. A system according to one of claims 44 to 81, wherein the holder is formed of a monolithic material.

101. A system according to one of claims 44 to 81, wherein the holder is constructed of two or more parts.

102. A system according to one of claims 44 to 81, wherein the holder includes a plurality of chemical indicators.

103. A system according to claim 102, wherein two or more of the plurality of chemical indicators are included in a location of the holder such that the first side is at least partially enclosed by the first reservoir.

104. A system according to claim 103, wherein the calibration solution is in contact with the two or more of the plurality of chemical indicators.

105. A system according to claim 102, wherein a first reservoir of the holder includes two or more separate reservoir chambers.

106. A system according to claim 105, wherein each of the two or more separate reservoir chambers includes one or more of the plurality of chemical indicators at least partially enclosed by the corresponding one of the two or more separate reservoir chambers.

107. A system according to claim 106, wherein each of the two or more separate reservoir chambers includes a feature as part of a corresponding portion of a selectively openable opening of the holder, the feature selected from the group consisting of a door, a cover, a valve, a foil cover, a paper cover, an aperture size and/or configuration designed to control fluid flow, a dissolvable membrane, a selectively dissolvable membrane (e.g., a biopolymer), and any combinations thereof.

108. A system according to claim 107, wherein the feature includes a foil cover removably covering the two or more separate reservoir chambers.

109. A system according to claim 107, wherein the feature includes a foil cover removably covering each of the two or more separate reservoir chambers.

110. A system according to one of claims 44 to 81, wherein the first optical reader includes a plurality of separate optical reading devices.

111. A system according to one of claims 44 to 81, wherein the first optical reader includes a component selected from the group consisting of a light pipe, a lens, an optical sensor, a light source, one or more optical lenses, one or more light pipes, one or more sensors capable of detecting light and/or other energy from a chemical indicator, a light source capable of producing a light and/or other energy for delivery to a chemical indicator, and any combinations thereof.

112. A system according to one of claims 44 to 81, wherein the one or more chemical indicators includes an indicator component selected from the group consisting of a dye, an immobilized dye, an immobilizing medium, an optical filtering film, an optical blocking film, and any combinations thereof.

113. A system according to claim 112, wherein the dye includes a dye selected from the group consisting of a calcium detecting aminonaphthalimide, a calcium detecting perylenediamide, a magnesium detecting dye based on a aminonaphthalimide, a magnesium detecting dye based on a photon induced electron transfer process (PET), a magnesium detecting dye based on a intramolecular charge transfer process (ICT), a magnesium detecting perylenediamide, a carbon dioxide sensitive dye based on a aminonaphthalimide, a carbon dioxide sensitive dye based on a photon induced electron transfer process (PET), a carbon dioxide sensitive dye based on a intramolecular charge transfer process (ICT), a carbon dioxide sensitive perylenediamide, and any combinations thereof.

114. A system according to claim 112, wherein the immobilizing medium includes a medium selected from the group consisting of a gel, a hydrogel, a solgel, an aerogel, a chalcogel, a polymer matrix, a cellulosic matrix, and any combinations thereof.

115. A system according to claim 114, wherein the dye includes a dye selected from the group consisting of a calcium detecting aminonaphthalimide, a calcium detecting perylenediamide, a magnesium detecting dye based on a aminonaphthalimide, a magnesium detecting dye based on a photon induced electron transfer process (PET), a magnesium detecting dye based on a intramolecular charge transfer process (ICT), a magnesium detecting perylenediamide, a carbon dioxide sensitive dye based on a aminonaphthalimide, a carbon dioxide sensitive dye based on a photon induced electron transfer process (PET), a carbon dioxide sensitive dye based on a intramolecular charge transfer process (ICT), a carbon dioxide sensitive perylenediamide, and any combinations thereof.

116. A system according to one of claims 44 to 81, wherein at least one of the one or more chemical indicators includes a dye covalently bonded to a cellulose fiber that is immobilized in a hydrogel.

117. A system according to claim 116, wherein the dye includes a dye selected from the group consisting of a calcium detecting aminonaphthalimide, a calcium detecting perylenediamide, a magnesium detecting dye based on a aminonaphthalimide, a magnesium detecting dye based on a photon induced electron transfer process (PET), a magnesium detecting dye based on a intramolecular charge transfer process (ICT), a magnesium detecting perylenediamide, a carbon dioxide sensitive dye based on a aminonaphthalimide, a carbon dioxide sensitive dye based on a photon induced electron transfer process (PET), a carbon dioxide sensitive dye based on a intramolecular charge transfer process (ICT), a carbon dioxide sensitive perylenediamide, and any combinations thereof.

118. A system according to one of claims 44 to 81, wherein the one or more chemical indicators includes a chemical indicator reactive for indicating a level of a constituent selected from the group consisting of pH, hardness, calcium, magnesium, oxygen, dissolved oxygen, carbon dioxide, ammonia, phosphate, nitrate, potassium, nitrite, carbon, a molecular organism, a metabolite, a mineral, an inorganic matter, an organic matter, a living organism, a conductive material, a heavy metal, a pathogen, and any combinations thereof.

119. A system according to one of claims 44 to 81, wherein the one or more chemical indicators includes an indicator that is reversible.

120. A system according to one of claims 44 to 81, wherein the one or more chemical indicators includes an indicator that is reactive based on a process that includes fluorescence, fluorescence decay, phase fluorescence, electromagnetic energy absorptance, electromagnetic energy absorbance, change in electromagnetic energy absorptance, change in electromagnetic energy absorbance, electromagnetic energy reflectivity, change in electromagnetic energy reflectivity, color, change in color, change in refractive index, refractive index, conductivity, change in conductivity, and any combinations thereof.

121. A method of calibrating an aquatic environment analysis system comprising: locating at least a portion of a first optical reader and at least a portion of a chemical indicator holder having a first chemical indicator in a first aquatic environment; aligning the first optical reader with a first side of the first chemical indicator wherein a second side of the first chemical indicator is in contact with a calibration solution and is reactive for indicating a level of a predetermined constituent of the first aquatic environment; using the first optical reader, measuring a first calibration reading of the second side in contact with the calibration solution, the measuring of the first calibration reading occurring through a fluid of the first aquatic environment in a first space between the first side and the optical reader, wherein the second side is not reactive to the predetermined constituent in the fluid in the first space; allowing the first aquatic environment to contact the second side without the calibration solution; using the first optical reader, measuring through the first aquatic environment in the first space an optical quality of the first indicator while the second side is in contact with the first aquatic environment; and calibrating the optical quality with the first calibration reading to obtain a value of a level of the predetermined constituent in the first aquatic environment.

122. A method according to claim 90, further comprising using the first optical reader and/or a second optical reader, measuring a second calibration reading of the fluid in the first space.

123. A method according to claim 91, wherein the measuring of the second calibration reading is performed while the second side is in contact with the calibration solution.

124. A method according to claim 91, wherein the measuring of the second calibration reading is performed while the second side is in contact with the first aquatic environment.

125. A method according to one of claims 91 to 124, wherein the calibrating the optical quality includes using the second calibration reading.

126. A method according to one of claims 90 to 124, wherein the locating includes submersing at least a portion of a first optical reader and at least a portion of a chemical indicator holder in the first aquatic environment.

127. A method according to one of claims 90 to 124, wherein said aligning includes connecting the holder to an optical sensing portion, wherein the first optical reader is part of the optical sensing portion.

128. A method according to claim 96, wherein said aligning includes moving the holder with respect to the first optical reader after the holder is connected to the optical sensing portion.

129. A method according to claim 96, wherein said connecting includes structurally connecting the holder to a hub of the optical sensing portion.

130. A method according to claim 96, wherein said connecting includes magnetically connecting.

131. A method according to claim 97, wherein said aligning includes moving the holder with respect to the first optical reader after the holder is connected to the optical sensing portion.

132. A method according to claim 97, wherein said connecting includes structurally connecting the holder to a hub of the optical sensing portion.

133. A method according to claim 132, wherein said aligning includes moving the holder with respect to the first optical reader after the holder is connected to the optical sensing portion.

134. A method according to one of claims 90 to 124, wherein the calibration solution is contained within a first reservoir of the holder.

135. A method according to claim 100, wherein the reservoir includes a first removable cover and wherein said allowing includes removing the cover from the reservoir to remove the calibration solution and allow a fluid of the first aquatic environment to enter the reservoir.

136. A method according to claim 101, further comprising: removing the holder from being in contact with the first aquatic environment prior to removing the cover; and replacing the holder to be in contact with the first aquatic environment to allow the fluid to contact the second side of the first chemical indicator.

137. A method according to claim 102, wherein the calibration solution is removed from the reservoir while the holder is not in contact with the first aquatic environment.

138. A method according to one of claims 90 to 124, wherein the calibration solution is removed from being in contact with the second side while the holder is in contact with the first aquatic environment.

139. A method according to one of claims 90 to 124, further comprising removing the holder from being in contact with the first aquatic environment, wherein the calibration solution is removed from being in contact with the second side while the holder is not in contact with the first aquatic environment.

140. A method according to one of claims 90 to 124, further comprising communicating the first calibration value and/or a raw value of the optical quality to a processor, wherein the calibrating is performed by the processor.

141. A method according to claim 106, wherein the processor is located in a location selected from the group consisting of within the optical sensing portion, connected to the optical sensing portion, in a computing device connected to the optical sensing portion, and any combinations thereof.

142. A method according to one of claims 90 to 124, further comprising providing the level of the predetermined constituent to a user of the analysis system via a display device.

143. A method of calibrating an aquatic environment analysis system, the aquatic environment analysis system including a first optical reader and a chemical indicator holder that is positioned opposite the first optical reader with a first space therebetween, the chemical indicator holder including at least one chemical indicator reactive for indicating a level of a predetermined constituent of a first aquatic environment, a first reservoir enclosing a first side of a first indicator of the at least one chemical indicators, and a calibration solution removably enclosed in the first reservoir and in contact with the first side, the method comprising: locating the first optical reader and at least a portion of the chemical indicator holder having the first indicator in the first aquatic environment; using the first optical reader, taking a first calibration reading of the first indicator while the first side is in contact with the calibration solution and a first portion of the first aquatic environment is in the first space; removing the calibration solution from the first reservoir; allowing a second portion of the first aquatic environment to contact the first side; using the first optical reader, measuring through the first aquatic environment in the first space an optical quality of the first indicator while the second portion is in contact with the first side; and calibrating the optical quality using the first calibration reading to obtain a value of a level of the predetermined constituent in the first aquatic environment.

144. A method of calibrating an aquatic environment analysis system comprising: submersing a first optical reader and at least a portion of a chemical indicator holder having a first chemical indicator in a first aquatic environment; aligning the first optical reader with a first side of the first chemical indicator wherein a second side of the first chemical indicator is in contact with a calibration solution and is reactive for indicating a level of a predetermined constituent of the first aquatic environment; using the first optical reader, measuring a first calibration reading of the second side in contact with the calibration solution, the measuring of the first calibration reading occurring through a fluid of the first aquatic environment in a first space between the first side and the optical reader, wherein the second side is not reactive to the predetermined constituent in the fluid in the first space; using the first optical reader and/or a second optical reader, measuring a second calibration reading of the fluid in the first space while the second side is in contact with the calibration solution; allowing the first aquatic environment to contact the second side without the calibration solution; using the first optical reader, measuring through the first aquatic environment in the first space an optical quality of the first indicator while the second side is in contact with the first aquatic environment; calibrating the optical quality with the first and second calibration readings to obtain a value of a level of the predetermined constituent in the first aquatic environment.