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WO2025226019A1 - Dispositif de réaction protéique et système de détection de protéine le comprenant - Google Patents

Dispositif de réaction protéique et système de détection de protéine le comprenant

Info

Publication number
WO2025226019A1
WO2025226019A1 PCT/KR2025/005448 KR2025005448W WO2025226019A1 WO 2025226019 A1 WO2025226019 A1 WO 2025226019A1 KR 2025005448 W KR2025005448 W KR 2025005448W WO 2025226019 A1 WO2025226019 A1 WO 2025226019A1
Authority
WO
WIPO (PCT)
Prior art keywords
protein
reaction
reaction solution
membrane
base
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/KR2025/005448
Other languages
English (en)
Korean (ko)
Inventor
박용원
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020250052355A external-priority patent/KR20250155478A/ko
Application filed by Individual filed Critical Individual
Publication of WO2025226019A1 publication Critical patent/WO2025226019A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Definitions

  • the present invention relates to a protein reaction device and a protein detection system including the same, and more particularly, to a protein reaction device based on the HRP sustained reaction method of an antibody-linked single protein and a protein detection system including the same.
  • the luminescent solution and the protein antibody HRP react, and using the camera of the protein detection system, the intensity and shape of the luminescence are measured at the membrane protein location, allowing the expression of a specific protein to be identified.
  • the protein to be measured can be prepared through the following process.
  • a sample is prepared through protein quantification, and the protein sample to be loaded by electrophoresis is separated according to size as large proteins move slowly through the network structure formed by acrylamide and small proteins move quickly.
  • Proteins are placed in a negatively charged SDS buffer and move through a network of acrylamide to the positive electrode.
  • proteins and size markers When proteins and size markers are placed in wells and voltage is applied to the gel, the proteins migrate at different speeds depending on their size. To separate small proteins, increase the acrylamide concentration, and to separate large proteins, decrease the acrylamide concentration. This separates proteins with large molecular weights.
  • the upper part of the electrophoresis is made by first making a gel with stacking buffer so that the proteins start from the same position, and then making a gel with a second running buffer so that they move under the same conditions.
  • proteins separated on a gel cannot bind to antibodies
  • detection using antibodies requires transferring the proteins from the gel to a membrane made of nitrocellulose or PVDF (polyvinylidene difluoride). This transfer is accomplished using running buffer or by applying electricity in a semi-dry manner.
  • PVDF polyvinylidene difluoride
  • the blocking process fills the empty space of the membrane, allowing only specific proteins to be detected cleanly and reducing nonspecific binding of antibodies to proteins.
  • a primary antibody that specifically binds to the protein to be detected is placed on the membrane and reacted with the protein, and then the membrane is reacted with a secondary antibody that has a luminescent function called HRP attached.
  • the unbound secondary antibody is washed away with wash buffer, and the protein band is measured with a camera using ECL solution to complete the protein experiment.
  • Western blot is an experimental method that measures the level of luminescence by attaching HRP to an antibody for a specific protein obtained from cells or tissues. After electrophoresis of the proteins to classify them by size, the proteins in the gel are transferred to a membrane, and then antigen-antibody + luminescent HRP are attached and the amount of light is measured to confirm the level of protein expression.
  • Western blot uses an antibody that reacts with the antigen epitope of the protein being searched for to find only the desired protein (antigen) in a sample protein mixture.
  • the proteins are transferred to a membrane such as PVDF or nylon.
  • the antigen-antibody reaction is used to find the specific protein to be found and measure the position and intensity of the luminescence in a protein experiment.
  • the problem to be solved by the present invention is to provide a protein reaction device capable of maintaining membrane activity and a protein detection system including the same.
  • a protein reaction device which comprises: an input portion for injecting a reaction solution into a reaction portion; and a reaction portion for inducing the reaction solution so that a membrane containing a protein reacts with the reaction solution, wherein the reaction portion comprises: a base including a plurality of water channels passing under the membrane; a transparent cover covering the membrane and the base to seal the water channels; and an inlet connected to the input portion between the base and the transparent cover, wherein the membrane is arranged between the base and the transparent cover.
  • the protein reaction device is characterized in that the input section injects the reaction solution into the reaction section using pneumatic pressure or hydraulic pressure, either manually or electrically.
  • the protein reaction device can have an input section that causes the reaction solution to flow in a forward direction away from the inlet using positive pressure, or in a direction approaching the inlet using negative pressure.
  • the protein reaction device may be configured such that the input portion includes a pump, and the reaction solution injected into the input portion is circulated back to the input portion through the action of the pump.
  • the protein reaction device may be configured such that the input portion includes a syringe.
  • the protein reaction device may be configured to further include a buffer section that prevents overflow of the reaction solution by introducing and withdrawing the reaction solution from the opposite side of the side where the inlet is located in the reaction section.
  • the protein reaction device is characterized in that the base is a plate-shaped device having a negative internal space composed of a plurality of channels formed horizontally in parallel so that a reaction solution flows; and a space where a membrane is placed on the channels.
  • the protein reaction device may be configured such that the base and the transparent cover each include a magnet, the base is coupled to the bottom of the imaging chamber using the attractive force of the magnet, and the transparent cover is aligned and coupled to the base using the attractive force of the magnet.
  • the protein reaction device may be configured to further include handles at both ends of the base and the transparent cover that are used for coupling and separating using the attractive force of the magnet.
  • the protein reaction device may be configured to further include a sealing portion disposed between the base and the transparent cover to prevent leakage of the reaction solution.
  • the protein reaction device may be configured to further include a connecting channel that functions as a buffer by controlling the flow rate during the inflow and outflow of the reaction solution between the input section and the reaction section.
  • the protein reaction device is characterized by having a reaction section having an S-shape in which a plurality of water channels flow back and forth in a zigzag manner between both ends of the membrane.
  • a protein detection system which comprises a protein reaction device that injects a reaction solution through an inlet, and continuously brings a new reaction solution into contact with a membrane containing a protein while reciprocating or circulating the reaction solution along a channel using air pressure to cause a reaction; an imaging system equipped with a camera that photographs the membrane; and a housing that accommodates the protein reaction device and the imaging system.
  • the luminescence signal measurement is weak and difficult to measure, or when the amount of protein is too large and the luminescence solution reacts excessively, the immediate surrounding reaction disappears and the signal turns gray or white, the color change can be prevented by continuously causing the reaction.
  • the luminescence occurs continuously, so that by using this patent, in cases where luminescence quickly disappears and measurement is difficult in the past, or even when the luminescent solution disappears due to excessive reaction with a large amount of protein, the signal can be measured with a camera by activating it so that luminescence continues.
  • the sensitivity can be increased by increasing the exposure time, which has the advantage of allowing measurement of proteins that could not be measured before.
  • FIG. 1 is a block diagram of a protein detection system including a protein reaction device according to one embodiment of the present invention.
  • Figure 2 is an exemplary diagram of a reaction section of a protein reaction device according to one embodiment of the present invention.
  • Figure 3 is an exploded view of the reaction unit of Figure 2.
  • Figure 4 is an exploded view of the reaction unit of Figure 2 viewed from a different angle than Figure 3.
  • Figure 5 is a plan view of the base constituting the reaction section of Figure 2.
  • Figure 6 is a cross-sectional view of the base of Figure 5.
  • Figure 7 is an example diagram of the input section of Figure 1.
  • Figure 8 is a connection diagram of a syringe among the base and input sections.
  • Figure 9 is a connection diagram of the pump among the base and input sections.
  • Fig. 10 is a connection diagram of a base and a syringe according to another embodiment.
  • Fig. 11 is a connection diagram of a base and a pump according to another embodiment.
  • Figure 12 is an example diagram of a reaction section over time.
  • Figure 13 is an example of a membrane in which continuous activation is maintained.
  • Figure 14 is an example of another membrane.
  • a component when a component is described as being "inside or connected to" another component, it should be understood that the component may be installed in direct connection with or in contact with the other component, may be installed spaced apart from the other component by a certain distance, and if installed spaced apart from the other component by a certain distance, there may be a third component or means for fixing or connecting the component to the other component, and the description of this third component or means may be omitted.
  • the solution is continuously supplied to the membrane in a dark room where light is blocked so that the reaction continues, even if the signal is weak, the reaction continues and the weakly reacting protein can be measured with a camera.
  • a protein reaction device (100) includes a configuration that causes a luminescence by contacting a luminescent solution (Enhenced Chemiluscence, ECL) with a membrane to which a protein emitting a weak or strong signal depending on the amount of protein is attached, and a configuration that automatically moves the ECL solution back and forth a certain distance to continuously activate the ECL solution on the membrane.
  • ECL Electrode Chemiluscence
  • the reaction solution may include an ECL solution.
  • the reaction solution may be created by mixing luminol and an oxidizing agent (hydrogen peroxide or another oxidizing agent).
  • Luminol is the primary agent in chemiluminescence reactions and reacts with the oxidizing agent to emit light.
  • the oxidizing agent reacts with luminol to induce chemiluminescence.
  • FIG. 1 is a block diagram of a protein detection system including a protein reaction device according to one embodiment of the present invention.
  • a protein detection system (10) may be configured to include a protein reaction device (100), an imaging system (200), and a housing (300).
  • the housing (300) is in a chamber form, and an imaging system (200) equipped with a camera (210) is installed inside the housing (300), and when the door is opened, the protein reaction device (100) can be placed on a shelf inside the housing (300).
  • the protein reaction device (100) has a function of continuously reacting a membrane containing proteins with a reaction solution until a reaction effect appears.
  • the protein reaction device (100) may be configured to include an input unit (110) and a reaction unit (130).
  • the input unit (110) has a function of injecting a reaction solution into the reaction unit (130).
  • the input unit (110) has a function of applying air pressure to the reaction solution to induce a reaction by continuously bringing the reaction solution into contact with a membrane provided inside the reaction unit (130).
  • the reaction unit (130) has a function of inducing the reaction solution to flow in both directions so that the reaction solution frequently comes into contact with the membrane.
  • the imaging system (200) has a function of capturing a reaction scene of a membrane and generating an image using a camera (210).
  • Figure 2 is an exemplary diagram of a reaction section of a protein reaction device according to one embodiment of the present invention.
  • Figure 3 is an exploded view of the reaction unit of Figure 2.
  • Figure 4 is an exploded view of the reaction unit of Figure 2 viewed from a different angle than Figure 3.
  • the reaction unit (130) may be configured to include a transparent cover (131) and a base (140).
  • the input section (110) will be described in detail in Fig. 7.
  • the transparent cover (131) has a function of covering the base (140) by being covered with the base (140). In addition, the movement of the reaction solution in the water channel (144) and the presence of proteins detected in the membrane can be observed through the transparent cover (131).
  • the transparent cover (131) and the base (140) are coupled to each other, and the reaction solution moves through the water channel (144) formed inside the base (140). That is, since the reaction solution exists between the transparent cover (131) and the base (140), a sealing part (134) can be installed between the transparent cover (131) and the base (140) to prevent the reaction solution from leaking to the outside.
  • the sealing part (134) can be made of rubber in the shape of an O-ring.
  • the base (140) includes a plurality of channels passing under the membrane.
  • An injection port (141) connected to the input unit (110) may be provided between the base (140) and the transparent cover (131).
  • a membrane may be placed between the base (140) and the transparent cover (131).
  • the transparent cover (131) must be able to be freely opened and closed with respect to the base (140).
  • the transparent cover (131) includes a magnet (136), and the base (140) also includes a magnet (137).
  • the two magnets (136, 137) at corresponding positions can be coupled to each other by the action of attraction.
  • handles (138) can be formed at both ends of the transparent cover (131) for the convenience of separation.
  • the magnet (137) formed on the base (140) can be coupled to a metal stand (135) on the bottom surface of the base (140) by attraction.
  • the stand (135) can be used for transportation.
  • the magnet (137) included in the base (140) can be attracted to the bottom of the shelf formed inside the housing (300) of the protein detection system (10).
  • Figure 5 is a plan view of the base constituting the reaction section of Figure 2.
  • the base (140) has a rectangular plate shape.
  • a plurality of channels (144) through which a reaction solution can flow are formed engraved on the base (140).
  • An inlet (141) through which the reaction solution is injected can be formed at one end of the base (140).
  • the inlet (141) is connected to the input unit (110).
  • the reaction solution injected into the injection port (141) first encounters the flow control unit (143) and then the connecting water channel (142).
  • the flow control unit (143) is a protrusion formed on the water channel and functions as a buffer by weakening the strong flow rate.
  • the connecting channel (142) is a type of storage space located between the input section (110) and the reaction section (130) and has the function of controlling the flow rate so that the reaction solution is evenly distributed to multiple channels (144).
  • the channel (144) corresponds to a passage through which the reaction solution flows. Since the reaction solution can flow across the membrane in the forward and backward directions along the channel (144), the reaction solution can continuously change direction and contact the membrane to increase the chance of reaction.
  • the depth of the channel can be determined according to the size of the reaction section (130) and the size of the membrane, and can be designed to have a depth of, for example, 0.3 mm.
  • the width of the channel (144) can be designed to have a width of 1 mm.
  • a membrane position (146) is positioned in the center of the water channel (144) where the membrane is positioned.
  • the membrane is in a floating state on the water channel (144), and the reaction solution flows through the upper and lower parts of the membrane.
  • a spill storage unit (145) may be placed on the outside of each water channel (144).
  • the spill storage unit (145) is a place to temporarily store a reaction solution that may spill due to a sealing failure.
  • Figure 6 is a cross-sectional view of the base of Figure 5.
  • a cross-section of the base (140) is depicted when cut vertically along the dashed line (A) shown in Fig. 5.
  • the water channel (144) is a passage through which the reaction solution flows.
  • a trench (147) may be formed between the water channels (144).
  • the space above the water channels (144) and the trench (147) is a floating space (148) where the membrane (M) is positioned. Since the membrane (M) adheres closely to the transparent cover (131) covering the base (140), there is no concern about it moving along with the reaction solution.
  • Figure 7 is an example diagram of the input section of Figure 1.
  • the input unit (110) injects the reaction solution into the reaction unit (130) using air pressure, hydraulic pressure, or a manual or electric method.
  • the input unit (110) may include a syringe, i.e., a syringe (111), or may include a syringe (111) and a driving device (112) that automatically drives the piston of the syringe (111).
  • the input unit (110) may include a mini pump (114) instead of the syringe (111).
  • the reaction solution may be injected and reciprocated or circulated across the membrane (M) along the water channel by means of air pressure by a syringe (111) operated by a human operator, a syringe (111) operated by a driving device (112), or a mini pump (114).
  • the driving device (112) can be configured to control the linear movement of the syringe (111) piston using the power of an electric motor.
  • a syringe (111) having a capacity of 1 ml to 20 ml can be used.
  • the membrane is placed and the protein reaction device (100) is placed in the housing and an image is measured, if the position of the membrane is misaligned, the image can be adjusted to be positioned horizontally in the correct position through an external program.
  • the driving device (112) includes a moving part switch, and when the moving part switch is turned on, the driving device (112) controls the piston of the syringe (111) to reciprocate, thereby allowing the reaction solution to continuously react with the membrane containing the protein.
  • Figure 8 is a connection diagram of a syringe among the base and input sections.
  • the syringe (111) can be connected to the injection port (141) formed in the base (140) through a tube (113).
  • Figure 9 is a connection diagram of the pump among the base and input sections.
  • the mini pump (114) can be connected to the injection port (141) formed in the base (140) through a tube (113). As a difference compared to the syringe (111), the mini pump (114) has the function of circulating the reaction solution.
  • Fig. 10 is a connection diagram of a base and a syringe according to another embodiment.
  • the plurality of water channels (154) engraved on the base (150) are characterized by an S-shape that allows water to flow back and forth in a zigzag manner between both ends of the membrane. That is, compared to the water channels in the same direction on the base (140), the water channels engraved on the base (150) are characterized by a narrower width and a longer length. The position of the buffer section (133) is depicted by a broken line.
  • the syringe (111) is connected to the injection port (151) through the tube (113), and the injection port (151) is connected to the connecting water channel (152) before reaching the water channel (154).
  • Fig. 11 is a connection diagram of a base and a mini pump according to another embodiment.
  • the mini pump (114) can circulate the reaction solution by connecting the inlet (151), which is the starting point of the water channel (154), and the end point of the water channel (154) through a tube (113).
  • Figure 12 is an example diagram of a reaction section over time.
  • a reaction unit (130) is depicted.
  • the process of a reaction solution injected from an inlet (141) (not shown) located on the right side of the reaction unit (130) flowing along a water channel (144) is depicted.
  • the reaction solution slowly permeates along the water channel (144), passes through a membrane (M), and reaches a buffer unit (133).
  • the buffer unit (133) prevents overflow while controlling the height of the reaction solution.
  • the buffer section (133) is in the form of a hollow column, the bottom surface of the space inside the column is connected to the water channel (144), and the top surface of the space is in contact with the air, so that when the reaction solution flows into the buffer section (133) through the water channel (144), the height of the reaction solution rises against the atmospheric pressure.
  • reaction solution When positive and negative pressures are alternately transmitted through the input unit (110), the reaction solution can move back and forth around the membrane (M), so that the reaction solution can continuously contact and react with the membrane (M).
  • Figure 13 is an example of a membrane in which continuous activation is maintained.
  • the image on the left is an image of a membrane according to a conventional technique
  • the image on the right is an image of a membrane obtained using a protein reaction device (100) according to an embodiment of the present invention. Comparing the two images, it can be seen that proteins can be detected more clearly in the present invention, where continuous activation is maintained.
  • a protein reaction device (100) is a method that can continuously cause a reaction to prevent color change in cases where the amount of linked primary and secondary antibodies is small because the luminescence reaction does not occur additionally due to a lack of reaction solution immediately below an existing membrane protein, or the luminescence disappears because the reaction occurs immediately, making it difficult to measure the luminescence signal because it is weak, or in cases where the amount of protein is too large and the luminescence solution reacts excessively, causing the immediate surrounding reaction to disappear and the signal to turn gray or white.
  • the signal can be measured with a camera by activating the luminescence to continue.
  • the sensitivity when measuring a membrane using a camera, the sensitivity can be increased by increasing the exposure time, so there is a great advantage in that it is possible to measure proteins that could not be measured before.
  • Figure 14 is an example of another membrane.
  • FIG. 14 images of proteins detected on a membrane are depicted when using vinyl according to a conventional technique and when using a protein reaction device (100) according to an embodiment of the present invention.
  • a protein reaction device 100 according to an embodiment of the present invention.
  • protein detection can be made more clearly according to the present invention than in the conventional technique.
  • the luminescence reaction when the luminescence reaction does not occur additionally due to insufficient reaction solution immediately below the existing membrane protein, or when the amount of linked primary and secondary antibodies is small, or when the reaction occurs immediately and luminescence disappears, or when the amount of protein is too large and the luminescence solution reacts excessively, and the surrounding reaction disappears immediately, the signal turns gray or white, etc., the measurement of the luminescence signal is weak and difficult to measure, and the signal can be continuously caused to occur so that it does not change.
  • the present invention can be used in the field of manufacturing a protein detection system.

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Abstract

La présente invention concerne un dispositif de réaction protéique comprenant : une partie d'entrée pour injecter une solution de réaction dans une partie de réaction ; et la partie de réaction pour induire la solution de réaction de telle sorte qu'une membrane contenant une protéine réagit avec la solution de réaction, la partie de réaction comprenant : une base comprenant une pluralité de canaux d'écoulement qui passent sous la membrane ; un couvercle transparent pour sceller les canaux d'écoulement en recouvrant la membrane et la base ; et un orifice d'injection relié à la partie d'entrée entre la base et le couvercle transparent, la membrane étant disposée entre la base et le couvercle transparent.
PCT/KR2025/005448 2024-04-23 2025-04-23 Dispositif de réaction protéique et système de détection de protéine le comprenant Pending WO2025226019A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20240053812 2024-04-23
KR10-2024-0053812 2024-04-23
KR1020250052355A KR20250155478A (ko) 2024-04-23 2025-04-22 단백질반응장치 및 이를 포함하는 단백질검출시스템
KR10-2025-0052355 2025-04-22

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WO2025226019A1 true WO2025226019A1 (fr) 2025-10-30

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US20090325223A1 (en) * 2008-06-24 2009-12-31 Timothy Boyd Magnetic immunohistochemical staining device and methods of use
KR20140110925A (ko) * 2012-01-09 2014-09-17 마이크로닉스 인코포레이티드. 마이크로유체 반응기 시스템
KR101616985B1 (ko) * 2015-08-31 2016-04-29 서울대학교산학협력단 비특이적 결합이 최소화된 나노입자 기반 체외진단 방법 및 반응용기
KR101741944B1 (ko) * 2016-01-12 2017-06-01 연세대학교 산학협력단 항원 농도 측정장치 및 항원 농도 측정방법
KR20230097964A (ko) * 2021-12-24 2023-07-03 한국과학기술원 진단을 위한 자동화 장치 및 이를 이용한 진단방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090325223A1 (en) * 2008-06-24 2009-12-31 Timothy Boyd Magnetic immunohistochemical staining device and methods of use
KR20140110925A (ko) * 2012-01-09 2014-09-17 마이크로닉스 인코포레이티드. 마이크로유체 반응기 시스템
KR101616985B1 (ko) * 2015-08-31 2016-04-29 서울대학교산학협력단 비특이적 결합이 최소화된 나노입자 기반 체외진단 방법 및 반응용기
KR101741944B1 (ko) * 2016-01-12 2017-06-01 연세대학교 산학협력단 항원 농도 측정장치 및 항원 농도 측정방법
KR20230097964A (ko) * 2021-12-24 2023-07-03 한국과학기술원 진단을 위한 자동화 장치 및 이를 이용한 진단방법

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