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WO2025207459A1 - Procédé de production de gélatine d'os - Google Patents

Procédé de production de gélatine d'os

Info

Publication number
WO2025207459A1
WO2025207459A1 PCT/US2025/021049 US2025021049W WO2025207459A1 WO 2025207459 A1 WO2025207459 A1 WO 2025207459A1 US 2025021049 W US2025021049 W US 2025021049W WO 2025207459 A1 WO2025207459 A1 WO 2025207459A1
Authority
WO
WIPO (PCT)
Prior art keywords
bone
solution
gelatin
produce
tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2025/021049
Other languages
English (en)
Inventor
Mohammad Z. ALBANNA
Nilabh S. KAJAVE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Humabiologics Inc
Original Assignee
Humabiologics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US18/614,665 external-priority patent/US12104088B1/en
Priority claimed from US18/901,009 external-priority patent/US12415848B1/en
Application filed by Humabiologics Inc filed Critical Humabiologics Inc
Publication of WO2025207459A1 publication Critical patent/WO2025207459A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
    • C08H1/00—Macromolecular products derived from proteins
    • C08H1/06—Macromolecular products derived from proteins derived from horn, hoofs, hair, skin or leather
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/10—Production of fats or fatty oils from raw materials by extracting
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein

Definitions

  • the stiffness and porosity of the methacrylated gelatin can be controlled by tuning the hydrogel concentration, degree of functionalization, and UV intensity wherein the bone gelatin is combined with a methacrylic anhydride to produce a bone gelatin methacrylic anhydride solution.
  • the bone gelatin methacrylic anhydride solution may be further mixed with a base solution such as sodium hydroxide solution in a specific pH range to produce bone methacrylate gelatin.
  • the pH range may be greater than 7.0 but less than 8.0, or less than about 7.5.
  • the bone gelatin methacrylic anhydride solution mixed with a sodium hydroxide solution may be centrifuged to produce the bone methacrylate gelatin.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Transplantation (AREA)
  • Dermatology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un procédé de production de gélatine d'os qui comprend une déminéralisation d'os à l'aide d'une solution d'acide de déminéralisation, l'os étant lavé pendant une durée de déminéralisation dans la solution de déminéralisation pour retirer les tissus mous. L'os déminéralisé est ensuite pulvérisé pour produire des particules de l'os déminéralisé dans un tissu osseux homogénéisé. Les lipides sont ensuite retirés du tissu osseux homogénéisé à l'aide d'une série d'étapes de lavage de retrait de lipides qui peuvent comprendre le lavage du tissu osseux homogénéisé dans une série de solutions, comprenant une solution de retrait de lipides et une solution éthanolique. La gélatine est ensuite retirée du tissu osseux à lipides retirés dans un procédé d'extraction de gélatine, le tissu osseux à lipides retirés étant placé dans une solution de pepsine suivie par une solution d'extraction. La gélatine provenant du tissu osseux à gélatine extraite peut ensuite être séparée, et la gélatine d'os peut ensuite être lyophilisée.
PCT/US2025/021049 2024-03-23 2025-03-22 Procédé de production de gélatine d'os Pending WO2025207459A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US18/614,665 US12104088B1 (en) 2023-03-23 2024-03-23 Bone gelatin process
US18/614,665 2024-03-23
US18/901,009 US12415848B1 (en) 2023-03-23 2024-09-30 Bone gelatin process
US18/901,009 2024-09-30

Publications (1)

Publication Number Publication Date
WO2025207459A1 true WO2025207459A1 (fr) 2025-10-02

Family

ID=97219804

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2025/021049 Pending WO2025207459A1 (fr) 2024-03-23 2025-03-22 Procédé de production de gélatine d'os

Country Status (1)

Country Link
WO (1) WO2025207459A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4294753A (en) * 1980-08-04 1981-10-13 The Regents Of The University Of California Bone morphogenetic protein process
WO2003020840A1 (fr) * 2002-09-06 2003-03-13 Perth Bone And Tissue Bank, Inc. Methodes de purification de gelatine d'os insoluble
CN102329843B (zh) * 2011-08-05 2013-09-04 安徽丰原集团有限公司 一种酶法制备明胶的方法
CN110655569B (zh) * 2019-10-29 2021-06-25 江南大学 一种制备骨明胶的方法
US12104088B1 (en) * 2023-03-23 2024-10-01 Humabiologics, Inc. Bone gelatin process

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4294753A (en) * 1980-08-04 1981-10-13 The Regents Of The University Of California Bone morphogenetic protein process
WO2003020840A1 (fr) * 2002-09-06 2003-03-13 Perth Bone And Tissue Bank, Inc. Methodes de purification de gelatine d'os insoluble
CN102329843B (zh) * 2011-08-05 2013-09-04 安徽丰原集团有限公司 一种酶法制备明胶的方法
CN110655569B (zh) * 2019-10-29 2021-06-25 江南大学 一种制备骨明胶的方法
US12104088B1 (en) * 2023-03-23 2024-10-01 Humabiologics, Inc. Bone gelatin process

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