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WO2025240164A1 - Procédés et dispositifs pour l'extraction, le nettoyage et l'enrichissement rationalisés d'analytes cibles à partir d'échantillons solides et semi-solides - Google Patents

Procédés et dispositifs pour l'extraction, le nettoyage et l'enrichissement rationalisés d'analytes cibles à partir d'échantillons solides et semi-solides

Info

Publication number
WO2025240164A1
WO2025240164A1 PCT/US2025/027946 US2025027946W WO2025240164A1 WO 2025240164 A1 WO2025240164 A1 WO 2025240164A1 US 2025027946 W US2025027946 W US 2025027946W WO 2025240164 A1 WO2025240164 A1 WO 2025240164A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample
vessel
extraction
cleaning
solid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2025/027946
Other languages
English (en)
Inventor
Gopal BERA
Sm Rahmat Ullah
German Augusto Gomez Rios
Husam Al-Esawi
Yan Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dionex Corp
Original Assignee
Dionex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dionex Corp filed Critical Dionex Corp
Publication of WO2025240164A1 publication Critical patent/WO2025240164A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0215—Solid material in other stationary receptacles
    • B01D11/0253—Fluidised bed of solid materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0215—Solid material in other stationary receptacles
    • B01D11/0253—Fluidised bed of solid materials
    • B01D11/0257—Fluidised bed of solid materials using mixing mechanisms, e.g. stirrers, jets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/028—Flow sheets
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4055—Concentrating samples by solubility techniques
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0203—Solvent extraction of solids with a supercritical fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0211—Solvent extraction of solids in combination with an electric or magnetic field
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2866—Grinding or homogeneising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4055—Concentrating samples by solubility techniques
    • G01N2001/4061—Solvent extraction

Definitions

  • the present disclosure generally relates to the field of analytical chemistry including methods and devices for streamline extraction, clean-up, and enrichment of target analytes from solid and semi-solid samples.
  • Pressurized liquid extraction or accelerated solvent extraction is a technique for extracting target analytes from solid and semisolid samples with liquid solvents.
  • Pressurized liquid extraction utilizes increased temperature and pressure with common solvents to increase the efficiency of the extraction process.
  • Pressurized liquid extraction can be used to replace more conventional Soxhlet, sonication, boiling, wrist-shaker, and other extraction methods.
  • pressurized liquid systems are currently offered by the Thermo Fisher Scientific including the ASETM 350 Accelerated Solvent Extractor and the EXTREVA ASETM Accelerated Solvent Extractor.
  • Pressurized liquid extraction methods performed by the ASETM extractors are accepted solid liquid extraction (SLE) methods useful for extraction of many types of analytes.
  • SLE solid liquid extraction
  • One such method is described in U.S. Pat. No. 5,843,311 (“the '311 patent”) and in EPA Method 3545.
  • An automated system for performing a pressurized liquid extraction method is described in U.S. Pat. No. 5,785,856 (“the '856 patent”).
  • An apparatus for parallel pressurized liquid extraction is described in U.S. Pat. No. 11,123,655 (“the ‘655 patent”).
  • the sample is generally first ground and/or mixed with a dispersing agent.
  • a weighed portion is placed in an extraction cell made of stainless steel or other materials, which is then heated to a predetermined temperature.
  • a conventional static valve is opened and solvent is pumped into the extraction cell until a target pressure is reached.
  • a second static valve is opened to allow the solvent with the extracted sample to flow into a collection vial.
  • a method of extracting a sample can include adding a sample to a disruption and dispersion (2D) vessel, the sample being solid or semi-solid; adding a collision object and a reagent to a sample in the 2D vessel; agitating the sample, collision object, and reagent in the extraction vessel to (a) break up larger material in the sample to increase available surface area for extraction and (b) mix the reagent with the sample to disperse the sample; and performing a fluidic extraction of the sample to isolate compounds of interest in a liquid phase separate from the sample matrix material.
  • 2D disruption and dispersion
  • performing the fluidic extraction can include supplying an extraction solvent at elevated pressure, elevated temperature, or a combination thereof.
  • the method can further include removing excess water from the sample.
  • removing excess water from the sample can include centrifuging the 2D vessel.
  • the method further includes adding the 2D vessel to a sample extraction vessel.
  • the method can further include adding a cleaning vessel to a sample extraction vessel in series with the 2D vessel.
  • the cleaning vessel can include one or more reagents for drying, defatting, or discoloration.
  • the cleaning vessel can include one or more analyte trapping reagent.
  • the method can further include an acetone wash to remove water from the sample where the analyte trapping reagent binds at least a portion of the compounds of interest prior to performing the fluidic extraction.
  • the method can further include concentrating the extracted compounds.
  • the method can further include analyzing the extracted compounds to identify and/or quantify the compounds of interest.
  • an extraction apparatus can include a disruption and dispersion (2D) vessel for containing a solid or semi-solid sample, one or more types of collision objects, and one or more reagents; a cleaning vessel containing one or more cleaning reagent; and a sample extraction vessel configured to hold the 2D vessel and at least one cleaning vessel in series.
  • 2D disruption and dispersion
  • the 2D vessel can include titanium, stainless steel, aluminum, cellulose, polymeric material, or any combination thereof
  • the cleaning vessel can include cellulose, stainless steel, aluminum, titanium, polymeric material, or any combination thereof.
  • the cleaning vessel can contain one or more reagents for drying, defatting, or discoloration.
  • the cleaning vessel can contain one or more analyte trapping reagents.
  • the sample extraction vessel can include titanium, stainless steel, aluminum, polymeric material, or any combination thereof.
  • the 2D vessel can be disposable.
  • the cleaning vessel can be disposable.
  • the sample extraction vessel can be disposable.
  • a disposable extraction vessel can include a bottom cap comprised of titanium, stainless steel, aluminum, cellulose, polymeric substances, or combinations thereof; a cell body comprised of cellulose, polymeric substances, titanium, stainless steel, aluminum, or combinations thereof; and a top cap comprised of cellulose, stainless steel, aluminum, or combinations thereof, wherein the disposable extraction vessel is configured to withstand pressures of between about 100 psi and about 2000 psi.
  • FIGs. 1 through 4 illustrate exemplary sample extraction vessel, an exemplary disruption and dispersion (2D) vessel, and an exemplary cleaning vessel, in accordance with various embodiments.
  • FIGs. 5 through 8 illustrate exemplary methods of sample extraction, in accordance with various embodiments.
  • a “system” sets forth a set of components, real or abstract, comprising a whole where each component interacts with or is related to at least one other component within the whole.
  • Samples containing significant amounts of water can present problems for pressurized solvent extraction techniques.
  • the water can be co-extracted with the sample into the solvent. This can result in an extracted sample having a different solution composition than intended, specifically containing more water than expected. This can interfere with techniques, such as evaporation, used to concentrate the sample. Additionally, in some cases, the co-extraction of the water can even be damaging to the analytical instruments, such as by damaging a chromatographic column through sudden shifts in solvent composition when a sample plug containing a high percentage of water is applied to a column. In other cases, the water may be immiscible in the solvent and result in two distinct phases, an aqueous phase and a solvent phase. This could result in the sample compounds partitioning between the phases and interfere with quantification of the compounds. As such, dewatering the sample before solvent extraction can improve the pressurized solvent extraction process.
  • Preparing the samples for analysis can include disruption, dispersion, drying, defatting, and discoloration, which is referred to a 5D process.
  • Disruption refers to the mechanical or chemical breakdown of sample matrices to enhance the release of target analytes. This process is vital for improving the efficiency of extraction and ensuring comprehensive analysis.
  • Dispersion denotes the mechanical process of ensuring the sample is homogeneous to enhance extraction efficiency. This may be achieved by introducing reagents or dispersing agents that prevent sample particles from clogging or forming agglomerations.
  • Drying refers to the removal of moisture from samples to facilitate subsequent analytical processes. This step is crucial for preventing clogging of fluidic lines and avoiding detrimental effects on analytical instrument performance. In the case of EXTREVA Accelerated Solvent Extractor, improper drying can interfere with the level sensing mechanism, leading to inaccurate results.
  • Defatting involves the extraction of lipids from samples to prevent interference with target analytes. This process is critical when dealing with tissue, biosolids, and other matrices where fat not only limits extraction but can cause ionization suppression and faster damage to components of the analytical instrument.
  • Discoloration pertains to the process of removing compounds, such as pigments, that generate color in the sample. This step is essential for ensuring that the sample does not retain any color that could interfere with subsequent analytical processes or the performance of the analytical instrument. Depending on the sample and the target analytes, one or more of these steps can be omitted.
  • FIG. 1 illustrates a sample extraction vessel 100 capable of handling solid and semi-solid samples.
  • Semi-solid samples are highly viscous materials with properties of both solids and liquids.
  • the sample extraction vessel 100 includes a top cap 102, a sample cell body 104, and a bottom cap 106.
  • Sample cell body 104 can include an interior space 108 for holding the sample.
  • the interior space 108 can be surrounded by an annular wall 1 10.
  • the annular wall 110 can be made of titanium, stainless steel, aluminum, polymeric material, or any combination thereof.
  • the interior of the annular wall 110 should be inert so as to not react with the sample.
  • the sample extraction vessel 100, or portions thereof can be disposable.
  • the annular wall 110 of the sample cell body 104 can be sufficiently rigid to withstand pressures of up to about 2000 psi used during extraction.
  • the sample cell body 104 can be pressured to an internal pressure of between about 100 psi and about 2000 psi during extraction, such as between about 100 psi to about 250 psi.
  • Various techniques can be used to couple the top cap 102 and the bottom cap 106 to the sample cell body 104, such as threaded attachments, magnetic attachments, snap-fit connections, bayonet mounts or other coupling mechanisms known in the art. A particular example is disclosed in US Patent 12,134,050.
  • FIG. 2 illustrates a disruption and dispersion (2D) vessel 200.
  • the 2D vessel 200 can include a top cap 202, a cell body 204, and a bottom cap 206.
  • Cell body 204 can include an interior space 208 surrounded by an annular wall 210.
  • the annular wall 210 can be made of metal, such as titanium, stainless steel, aluminum, cellulose, polymeric material, or any combination thereof.
  • the interior of the annular wall 210 should be inert so as to not react with the sample.
  • the annular wall 210 of the 2D vessel 200 can be able to withstand pressures of up to about 2000 psi used during extraction, in other embodiments, the annular wall 210 of the 2D vessel 200 can be supported by the annular wall 110 if sample extraction vessel 100 to provide sufficient rigidity to withstand the elevated pressures. In various embodiments, the 2D vessel 200 can be able to withstand the forces during centrifugation. In some embodiments, the 2D vessel 200, or portions thereof, can be disposable. In various embodiments, the top cap 202 and the bottom cap 206 can include a screen or membrane to allow the flow of gas and liquid but retain solid material, such as particles above a certain size, such as at least about 0.02 pm.
  • the sample can be added to the interior space 208 along with collision objects and a reagent to assist with water removal (drying), disruption and dispersion
  • the 2D vessel 200 can be sized to fit into a disposable centrifuge tube. In various embodiments, excess water can be removed from a sample by centrifuging the sample within the 2D vessel 200 in the disposable centrifuge tube.
  • FIG. 3 illustrates a cleaning vessel 300.
  • the cleaning vessel 300 can include a top cap 302, a cell body 304, and a bottom cap 306.
  • Cell body 304 can include an interior space 308 surrounded by an annular wall 310.
  • the annular wall 310 can be made of cellulose, stainless steel, aluminum, titanium, polymeric material, or any combination thereof.
  • the interior of the annular wall 310 should be inert so as to not react with the sample.
  • the annular wall 310 of the cleaning vessel 300 can be able to withstand pressures of up to about 2000 psi used during extraction, in other embodiments, the annular wall 310 of the cleaning vessel 300 can be supported by the annular wall 110 if sample extraction vessel 100 to provide sufficient rigidity to withstand the elevated pressures.
  • the cleaning vessel 300 can be disposable.
  • the top cap 302 and the bottom cap 306 can include a screen or membrane filter to allow the flow of liquid but retain solid material, such as particles above a certain size, such as at least about 0.02 pm.
  • the interior space 308 of the cleaning vessel 300 can be filled with one or more reagents that can be used for drying, defatting, and/or discoloration of the sample extract.
  • the cleaning vessel 300 can be prefilled with the one or more reagents, while in other embodiments, the one or more reagents can be provided separately and may need to be added to the cleaning vessel 300 before use.
  • multiple cleaning vessels 300 can be used in sequence to ensure proper drying, defatting, and discoloration of the sample extract.
  • the multiple cleaning vessels 300 can contain the same mix of reagents, while in other embodiments, each cleaning vessel 300 can include different reagents, such as using a drying cleaning vessel, a defatting cleaning vessel, and a discoloration cleaning vessel.
  • FIG. 4 illustrates an extraction assembly 400 including a sample extraction vessel 100, a 2D vessel 200, and a cleaning vessel 300.
  • the inner diameter of the sample extraction vessel 100 can be slightly larger than the outer diameter of the 2D vessel 200 and the cleaning vessel 300 to allow for the 2D vessel 200 and the cleaning vessel 300 to be placed inside the sample extraction vessel 100.
  • the sample extraction vessel 100 can hold at least one 2D vessel 200 and at least one cleaning vessel 300.
  • FIG. 5 is a flow diagram 500 illustrating a method 500 of the extraction of a sample.
  • the sample can include between about 0% and about 75% water content.
  • the sample can include between 0% and 60% lipid content.
  • FIGs. 6 and 7 provide further illustration of the method 500.
  • a sample is provided in 2D vessel, such as 2D vessel 200.
  • an operator may attach a bottom cap on one end of the cell body and add solid or semi-solid material to the 2D vessel.
  • the 2D vessel can be centrifuged to remove excess water, as illustrated in FIG. 7. In other embodiments, it may not be necessary to remove excess water, as illustrated in FIG. 6.
  • collision objects and reagent can be added to the 2D vessel.
  • the reagent can include reagents to assist with disruption and dispersion.
  • the reagents can also assist with analyte desolvation, water removal (drying), lipid removal (defatting), and pigment removal (discoloration).
  • the 2D vessel can be agitated to dry, disrupt, and disperse the sample. Through agitation, the collision objects can break down the sample into finer material and the reagent and the sample can be thoroughly mixed.
  • the 2D vessel can be placed in an extraction vessel along with a cleaning vessel to form an extraction assembly.
  • the extraction assembly can include multiple cleaning vessels.
  • the extraction assembly can be placed in an extraction system, and at 514, a liquid extraction can be performed.
  • the extraction system can be a liquid extraction system, pressurized liquid extraction system, supercritical extraction system, Soxhlet extraction system, microwave assisted extraction system, or combinations thereof.
  • the extraction system acts to extract the compounds of interest from the sample into a liquid solvent.
  • the compounds of interest can include polyaromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), dioxins, per-and polyfluoroalkyl substances (PF AS), organochlorine pesticides (OCPs).
  • PAHs polyaromatic hydrocarbons
  • PCBs polychlorinated biphenyls
  • PF AS per-and polyfluoroalkyl substances
  • OCPs organochlorine pesticides
  • the extracted compounds can optionally be concentrated, such as by evaporation of the extraction solvent.
  • the sample can be analyzed, such as to determine the identity and concentration of the compounds of interest.
  • the compounds can be analyzed by spectrometry, mass spectrometry, chromatography, including gas chromatography, liquid chromatography, and ion chromatography, other analytical techniques known in the art for analysis and identification of compounds, or any combination thereof.
  • water can be removed using acetone while the compounds of interest, such as PAHs, SVOC, and PFAS, are retained by an absorbing material.
  • the absorbing material can include nonimprinted polymer (NIP), molecularly imprinted polymer (MIP), or hyper-crosslinked polymer (HCP).
  • NIP nonimprinted polymer
  • MIP molecularly imprinted polymer
  • HCP hyper-crosslinked polymer
  • the absorbing material can be used to retain the analyte in the sample can also include various polymeric materials such as XAD-2, PS-DVB, HLB, polymethacrylate, polystyrene-divinylbenzene, and polyacrylate. These materials are chosen for their ability to effectively capture and hold target analytes while allowing the solvent, acetone, to carry away water and other impurities.
  • the main purpose of this step is to place a suitable material in the cleaning vessel that will retain the target analytes, ensuring that the extraction process is efficient, and the analytes are isolated for further analysis.
  • a suitable material in the cleaning vessel that will retain the target analytes, ensuring that the extraction process is efficient, and the analytes are isolated for further analysis.

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  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un procédé d'extraction d'un échantillon, le procédé comprenant l'ajout d'un échantillon à un récipient de rupture et de dispersion (2D), l'échantillon étant solide ou semi-solide ; l'ajout d'un objet de collision et d'un réactif à un échantillon dans le récipient 2D ; l'agitation de l'échantillon, de l'objet de collision, et du réactif dans le récipient d'extraction pour (a) briser un matériau plus gros dans l'échantillon en vue d'augmenter la surface disponible pour l'extraction et (b) mélanger le réactif avec l'échantillon en vue de disperser l'échantillon ; et la réalisation d'une extraction fluidique de l'échantillon pour isoler des composés d'intérêt dans une phase liquide séparée du matériau de matrice d'échantillon.
PCT/US2025/027946 2024-05-17 2025-05-06 Procédés et dispositifs pour l'extraction, le nettoyage et l'enrichissement rationalisés d'analytes cibles à partir d'échantillons solides et semi-solides Pending WO2025240164A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202463649305P 2024-05-17 2024-05-17
US63/649,305 2024-05-17

Publications (1)

Publication Number Publication Date
WO2025240164A1 true WO2025240164A1 (fr) 2025-11-20

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Country Status (1)

Country Link
WO (1) WO2025240164A1 (fr)

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5785856A (en) 1994-06-14 1998-07-28 Dionex Corporation Automated accelerated solvent extraction apparatus and method
US5843311A (en) 1994-06-14 1998-12-01 Dionex Corporation Accelerated solvent extraction method
US20050178726A1 (en) * 2004-02-18 2005-08-18 Robert Belly Disruption of cells and tissues
US20140224732A1 (en) * 2013-02-13 2014-08-14 Kannan Srinivasan Apparatus for parallel accelerated solvent extraction
US9375697B2 (en) * 2014-03-12 2016-06-28 Dionex Corporation Extraction cell assembly with quick-release seal removal
US20160349158A1 (en) * 2012-08-30 2016-12-01 Dionex Corporation Method and device to extract an analyte from a sample with gas assistance
WO2019090137A1 (fr) * 2017-11-03 2019-05-09 Cdx Analytics, Llc Procédés, trousses et systèmes pour l'homogénéisation et l'analyse d'échantillons
US20200054962A1 (en) * 2018-08-17 2020-02-20 Evello International, LLC Systems and methods of cannabis oil extraction
US20240091669A1 (en) * 2022-09-20 2024-03-21 Saudi Arabian Oil Company Semi-automatic crystallization process testing
US12134050B2 (en) 2020-09-30 2024-11-05 Dionex Corporation Extraction cell cap assembly with toolless disassembly

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5785856A (en) 1994-06-14 1998-07-28 Dionex Corporation Automated accelerated solvent extraction apparatus and method
US5843311A (en) 1994-06-14 1998-12-01 Dionex Corporation Accelerated solvent extraction method
US20050178726A1 (en) * 2004-02-18 2005-08-18 Robert Belly Disruption of cells and tissues
US20160349158A1 (en) * 2012-08-30 2016-12-01 Dionex Corporation Method and device to extract an analyte from a sample with gas assistance
US20140224732A1 (en) * 2013-02-13 2014-08-14 Kannan Srinivasan Apparatus for parallel accelerated solvent extraction
US11123655B2 (en) 2013-02-13 2021-09-21 Dionex Corporation Apparatus for parallel accelerated solvent extraction
US9375697B2 (en) * 2014-03-12 2016-06-28 Dionex Corporation Extraction cell assembly with quick-release seal removal
WO2019090137A1 (fr) * 2017-11-03 2019-05-09 Cdx Analytics, Llc Procédés, trousses et systèmes pour l'homogénéisation et l'analyse d'échantillons
US20200054962A1 (en) * 2018-08-17 2020-02-20 Evello International, LLC Systems and methods of cannabis oil extraction
US12134050B2 (en) 2020-09-30 2024-11-05 Dionex Corporation Extraction cell cap assembly with toolless disassembly
US20240091669A1 (en) * 2022-09-20 2024-03-21 Saudi Arabian Oil Company Semi-automatic crystallization process testing

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