[go: up one dir, main page]

WO2025137016A1 - Réseau à surface réduite de cartouches de réactif pré-aliquoté pour un système de préparation de banques - Google Patents

Réseau à surface réduite de cartouches de réactif pré-aliquoté pour un système de préparation de banques Download PDF

Info

Publication number
WO2025137016A1
WO2025137016A1 PCT/US2024/060632 US2024060632W WO2025137016A1 WO 2025137016 A1 WO2025137016 A1 WO 2025137016A1 US 2024060632 W US2024060632 W US 2024060632W WO 2025137016 A1 WO2025137016 A1 WO 2025137016A1
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
cartridges
array
reagent cartridges
cartridge
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/060632
Other languages
English (en)
Inventor
Gregory HOLST
James Osmus
Bryan Crane
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of WO2025137016A1 publication Critical patent/WO2025137016A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/02—Integrated apparatus specially adapted for creating libraries, screening libraries and for identifying library members
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1002—Reagent dispensers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0418—Plate elements with several rows of samples

Definitions

  • the present application generally relates to library preparation systems, and more specifically to library preparation systems including an array of pre-aliquoted reagent cartridges having an area reduced footprint.
  • Library preparation systems generally include working areas where an assay is stored, consumable areas for storing additives, such as reagents and other consumables used for library preparation, and loading areas, for loading and/or transferring a prepared sample to a system such as a sequencing system.
  • Each of these areas generally includes an array of containers or cartridges that hold the various components (e.g., samples, reagents, disposable tips, etc.).
  • Increasing the capacity of a library preparation system includes adding more containers or cartridges to the array, which increases the area or footprint of the array in the system, and thus the area of the system itself.
  • a library preparation system may generally include consumables areas, working areas, and loading areas.
  • the working area may be referred to as an assay bay or a plurality of assay bays (e.g., first and second assay bays).
  • the consumable areas may be used to load and store reagents and consumables needed for the library preparation process, the consumables may include, but are not limited to, disposable tips, wet or dry assay specific reagents, wet or dry bulk reagents, and reaction plates and wells.
  • the consumables area may include a consumables receptacle which may include a tray configured to hold an array of reagent cartridges or containers.
  • an array of reagent cartridges for a library preparation system includes a plurality of reagent cartridges, each reagent cartridge in the plurality of reagent cartridges having a central volume axis, a cross-sectional major axis and a cross-sectional minor axis, and each reagent cartridge in the plurality of reagent cartridges being configured to be accessible by a pipette of a library preparation system.
  • Each reagent cartridge in the plurality of reagent cartridges is spaced from an adjacent reagent cartridge in the array in an X (or first) direction and in a Y (or second) direction, and the spacings in the X direction between adjacent reagent cartridges are equal and the spacings in the Y direction between adjacent reagent cartridges are equal, but spacing between adjacent reagent cartridges in the X direction are unequal to spacings between adjacent cartridges in the Y direction.
  • a library preparation system includes an assay bay, the assay bay being configured to prepare a library of samples, a consumable area including a reagent cartridge receptacle, and an array of reagent cartridges disposed in the reagent cartridge receptacle.
  • the array or reagent cartridges includes a plurality of reagent cartridges, each reagent cartridge in the plurality of reagent cartridges having a central volume axis and a cross-sectional major axis and a cross-sectional minor axis.
  • the system may further include or comprise any one or more of the following features.
  • the major axis of at least one reagent cartridge of the plurality of reagent cartridges is larger than the minor axis.
  • an aspect ratio of at least one reagent cartridge of the plurality of reagent cartridges is less than 3:1 .
  • the aspect ratio of at least one reagent cartridge of the plurality of reagent cartridges is less than 2:1 .
  • At least one reagent cartridge of the plurality of reagent cartridges has an oval-shaped cross-section.
  • at least one reagent cartridge of the plurality of reagent cartridges has an ellipse-shaped cross-section.
  • At least one reagent cartridge of the plurality of reagent cartridges has a rectangular-shaped cross-section.
  • a first reagent cartridge of the plurality of reagent cartridges has a different shape from a second reagent cartridge of the plurality of reagent cartridges.
  • a first reagent cartridge of the plurality of reagent cartridges has a different size from a second reagent cartridge of the plurality of reagent cartridges.
  • a first reagent cartridge of the plurality of reagent cartridges has a circular cross-sectional shape with a first diameter and a second reagent cartridge of the plurality of reagent cartridges has a second diameter, the first diameter being larger than the second diameter.
  • spacing between adjacent reagent cartridges of the plurality of reagent cartridges in the X direction is larger than spacing between adjacent reagent cartridges in the Y direction.
  • a pipette is included and each reagent cartridge in the plurality of reagent cartridges is configured to be accessible by the pipette.
  • the pipette is mounted on a cross bay gantry and the pipette is movable in an X direction and in a Y direction.
  • the pipette has a plurality of pipette tips.
  • the assay bay comprises a plurality of sample wells.
  • FIG. 1 is a top view of a library preparation system.
  • FIG. 2 is a front view of the system of FIG. 1 .
  • FIG. 3 is an isometric view of the system of FIG. 1 .
  • FIG. 4B is a second example of a prior art reagent reservoir arrangement in combination with sample wells;
  • FIG. 4C is a generic representation of the prior art reagent reservoir arrangement of FIG. 4B, but without the sample reservoirs for ease of comparison;
  • FIG. 5A is a first example of an array of reagent reservoirs constructed in accordance with the disclosure.
  • FIG. 5B is a second example of an array of reagent reservoirs constructed in accordance with the disclosure.
  • FIG. 5C is a third example of an array of reagent reservoirs constructed in accordance with the disclosure.
  • FIG. 6 illustrates a schematic diagram of the library preparation system of claim 1 , including a control system.
  • At least one aspect of this disclosure is related to arrays of reagent cartridges or containers that increase cartridge or container density without increasing the overall area occupied by the array of reagent cartridges or containers.
  • Anther aspect of the disclosure is related to arrays of reagent cartridges or containers that have a reduced area footprint, relative to existing arrays of reagent cartridges or containers having the same number of individual cartridges or containers.
  • the disclosed implementations increase throughput or capacity of a library preparation system by allowing more reagent cartridges or containers to be contained in an existing area footprint limitation.
  • the disclosed implementations of arrays of reagent cartridges or containers allow a library preparation system to process larger numbers of samples requiring larger numbers of individual or unique reagents for processing, without increasing the area footprint of the existing library preparation system.
  • the size of traditional liquid handling instruments of the library system is typically driven by the number of reagents, the number of reaction reservoirs, and the number of pipette tips required by the assay. These consumables are typically laid out in a horizontal plane, placed side-by-side, and are accessible to a pipette riding on an XYZ gantry, as illustrated in FIG. 1 , for example.
  • This arrangement forces the overall dimensions of the instruments to increase when supporting more complicated workflows with more reagents, wells, and tips, which requires a larger area size footprint for the library preparation system.
  • the area footprint of the library preparation system is practically limited by the size of a lab bench or other working surface on which the library system is disposed.
  • a library preparation system 200 may include one or more consumables areas 302, a first working area 304 optionally having two working bays 304A, 304B, an optional second working area 306, and a loading area.
  • the first working area 304 may be referred to as an assay bay and the second working area 306 may be referred to as a common resource bay.
  • the library preparation system may include a single working area 304.
  • the consumable areas 302 may include one or more consumable receptacles that may be used to load and store reagents and consumables needed for a library preparation process, including, disposable tips, wet or dry assay specific reagents, wet or dry bulk reagents, and reaction plates and wells.
  • the consumables, such as reagent are typically stored in an array, for example an array 111 of individual reagent cartridges 154.
  • previous attempts to reduce footprint size of reagent cartridges have included loading reagents into bulk reservoirs 54 and then distributing each reagent to each sample well 24 at the time-of-use serially (only eight sample wells are illustrated for clarity, but typical library systems may have twenty four or more sample wells).
  • the first regent is delivered from the first reagent reservoir 54 to each sample well 24, then the second reagent is delivered from the second reagent reservoir 54 to each sample well 24, and so on, in a serial nature, depositing each reagent in each sample well 24 one at a time. All sample wells 24 are loaded with the first reagent before the second reagent is loaded.
  • FIG. 4B another known reagent organizational approach is illustrated that reduces run time by reducing the number of movements required by a pipette (not shown in FIG. 4B).
  • run time may be significantly reduced by using a multi-channel pipette to pipette reagents to all sample wells 24 (eight sample wells are illustrated, but as stated earlier, twenty four or more sample wells are commonly used), in parallel, thus reducing the number of movements required by the pipette to deliver reagents to the sample wells 24.
  • spacing S x between central axes B of adjacent reagent cartridges 54 in the X direction is equal to spacing S y of adjacent reagent cartridges 54 in the Y direction.
  • the central axes B of the reagent cartridges 54 are spaced approximately 9 mm from one another and the entire array 11 of reagent cartridges 54 measures approximately 37 mm in a Y direction and approximately 79 mm in the X direction. This standard spacing allows standardized manufacturing equipment with 9 mm pitch pipettes to fill the consumables.
  • the Y direction of the array 111 is reduced in three ways in the illustrated examples, 1) by changing the shape of the well to a shape having a smaller Y dimension then an X dimension, such as an ellipse or an oval, rather than a circle, to maintain a smooth perimeter with no corners as illustrated in FIG. 5B; 2) by changing the well to an angled or non-circular shape having a smaller Y dimension than an X dimension, such as a rectangular shape or a polygon, to make better use of the available surface area, as illustrated in FIG. 5B; and 3) by reducing the size of certain circular wells when only a small volume is needed, and maintaining standard sized wells when more volume is needed, as illustrated in FIG. 5C.
  • the reagent cartridge 154 shapes have a major axis Mi and a minor axis M 2 .
  • the major axis Mi is larger than the minor axis M 2 .
  • Both the major axis Mi and the minor axis M 2 are symmetrical axes for the cross-sectional shape of the reagent cartridge 154.
  • the shapes may be mixed.
  • some rows in the array 111 may include oval or ellipse shaped reagent cartridges 154 while other rows in the array 111 may include rectangular or polygonal shaped reagent cartridges.
  • One benefit of the disclosed arrays of reagent cartridges is, however, that the proposed arrays of reagent cartridges maintain a standard and uniform X direction separation (e.g., in some implementations about a 9 mm separation) between individual reagent cartridges in the array in the X direction so the risk of cross contamination is not increased as it would in a consumable with a smaller pitch, such as a 4.5 mm pitch in the X direction.
  • a standard and uniform X direction separation e.g., in some implementations about a 9 mm separation
  • the arrays of reagent cartridges could be further modified to reduce spacing in the X direction.
  • smaller reagent cartridges for example the smaller reagent cartridges 154 illustrated in FIG. 5C could be packed more closely (e.g., hexagonal close pack) to further reduce the Y dimension of the array.
  • the aspect ratio of the reagent cartridge affects mixing performance.
  • an aspect ratio between the X and Y dimensions cross- sectional or top view
  • more specifically a ratio between the major axis Mi and the minor axis M 2 of the reagent cartridge 154 is less than about 3:1 and preferably less than about 2:1
  • mixing problems are reduced.
  • the longer and narrower nature of reagent cartridges having an aspect ratio larger than about 3:1 inhibits mixing, particularly when the pipette cannot move in the X direction and cannot reach the ends of the longer and narrower reagent cartridge.
  • FIG. 6 a schematic diagram of an implementation of a library preparation system 100 that may include the above described arrays 111 of reagent cartridges 154 is illustrated.
  • the exemplary library preparation system may optionally include a system for managing the library preparation system.
  • the described optional system for managing the library preparation system is not required to implement the disclosed arrays 111 of reagent cartridges 154 and the arrays 111 of reagent cartridges 154 may be implemented in other library preparation systems.
  • the system 100 may be used to automatically, easily, and efficiently prepare DNA libraries for sequencing applications, for example.
  • the system 100 may prepare the libraries when performing workflows such as whole genome sequencing workflows, DNA & RNA enrichment workflows, methylation workflows, split-pool amplicon workflows, amplicon workflows.
  • the workflows may include one or more steps, such as amplification processes, cleanup processes, quantification processes, library normalization processes, pooling processes, denaturing processes, and/or diluting processes in some implementations.
  • the system may include a controller 176.
  • the controller may be electrically and/or communicatively coupled to one or more components of the system 100 to perform various functions as described herein.
  • the controller 176 may include a user interface 221 , a communication interface 222, one or more processors 224, and a memory 226 storing instructions executable by the one or more processors 224 to perform the various functionalities described herein.
  • the user interface 221 may receive input from a user, and provide information to the user associated with the operation of the system 100 (e.g., information about the workflows being scheduled and/or taking place).
  • the user interface 221 may include a touch screen, a display, a key board, a speaker(s), a mouse, a track ball, and/or a voice recognition system.
  • the touch screen and/or the display may display a graphical user interface (GUI).
  • GUI graphical user interface
  • the system 100 may include the communication interface 222 to enable communication between the system 100 and one or more components, such as a remote system(s) (e.g., computers) using one or more network(s).
  • the network(s) may include an intranet, a local-area network (LAN), a wide-area network (WAN), the intranet, etc.
  • Some of the communications may be associated with workflows of the system 100, such as scheduling one or more workflows.
  • the system 100 may include one or more processors 224 and/or processorbased system(s) or a microprocessor-based system(s).
  • the one or more processors 224 and/or the system 100 includes a reduced-instruction set computer(s) (RISC), an application specific integrated circuit(s) (ASICs), a field programmable gate array(s) (FPGAs), a field programmable logic device(s) (FPLD(s)), a logic circuit(s), and/or another logic-based device executing various functions including the ones described herein.
  • RISC reduced-instruction set computer
  • ASICs application specific integrated circuit
  • FPGAs field programmable gate array
  • FPLD(s) field programmable logic device
  • the user interface 221 , the communication interface 222, and the memory 226 may be electrically and/or communicatively coupled to the one or more processors 224.
  • the memory 226 may include one or more of a hard disk drive, a flash memory, a read-only memory (ROM), erasable programmable read-only memory (EPROM), electrically erasable programmable read-only memory (EEPROM), a random-access memory (RAM), non-volatile RAM (NVRAM) memory, a compact disk (CD), a digital versatile disk (DVD), a cache, and/or any other storage device or storage disk in which information is stored for any duration (e.g., permanently, temporarily, for extended periods of time, for buffering, for caching, etc.).
  • ROM read-only memory
  • EPROM erasable programmable read-only memory
  • EEPROM electrically erasable programmable read-only memory
  • RAM random-access memory
  • NVRAM non-volatile RAM
  • CD compact disk
  • DVD digital versatile disk
  • cache and/or any other storage device or storage disk in which information is stored for any duration (e.g., permanently, temporarily, for extended periods of time
  • the memory 226 may include a scheduler 227.
  • the scheduler 227 may be stored as instructions on the memory 226 and executable by the processor 224.
  • the scheduler 227 may include a common resource schedule which is used to schedule and/or execute (e.g., by the controller 176) one or more workflows.
  • the scheduler 227 may receive a workflow request associated with one or more working areas 304, 306 (e.g., assay bay, common resource, etc.), and schedule the workflow to a common resource schedule.
  • the common resource schedule may be used schedule and/or execute (e.g., via controller 176) one or more workflows which utilize the second working area 306 (e.g., common resource).
  • the system 100 may include one or more consumables areas 302, 309, a first working area 304, a second working area 306, and a loading area 308.
  • the first working area 304 may be referred to as an assay bay (e.g., first and second assay bays) and the second working area 306 may be referred to as a common resource.
  • the consumable areas 302 may be used to load and store reagents and consumables needed for a library preparation process, including, disposable tips, wet or dry assay specific reagents, wet or dry bulk reagents, and reaction plates and wells.
  • the consumables area 302 may include a consumables receptacle 310 which may include a tip tray 114 having a first tip 116 and a second tip 118, a first plate 120 having a well 122 containing a sample 124, and a second plate 126 having a well 128.
  • one or more of the working areas 304, 306 may include a consumables area.
  • the second working area 306 includes consumables area 309, which may include a tip tray 114 and a third plate 142 having a well 143.
  • the consumables receptacle 310 may be a drawer that can be pulled out from the system 100 and loaded with the consumables.
  • the consumables receptacle 310 may also include a lid 130, an index tray 132 having a well 134 containing indexes 136, a bead tray 138 having a well 140 containing beads 141 , a liquid reservoir 312, and a dry reagent reservoir 314.
  • One or more of these reagents 136 and/or 141 may be lyophilized and included with the dry reagent reservoir 314.
  • one or more of the plates e.g., plates 120, 126
  • the index tray 132, and/or the bead tray 138 may be stacked.
  • the tip tray 114 may have a plurality of the first tips 116, a plurality of the second tips 118, and/or any suitable number of tips 116, 118.
  • the tips 116, 118 may be different sizes, may be reusable for at least multiple portions of a workflow.
  • the system 100 may include the first working area 304, which may include a contact dispenser 145, a stage 148, a magnet 150, and a thermocycler 152.
  • the system 100 may include the second working area 306 having an analyzer area 154 (e.g., to analyze samples), a contact dispenser 318 and a stage 320, one or more of which can operate in and/or in conjunction with the first working area 304 and the second working area 306.
  • the contact dispenser 145 may be movable to aspirate / dispense liquid to the consumables area 302 and/or to the first working area 304.
  • the contact dispenser 318 may be movable to aspirate / dispense liquid to the consumables area 302, to the first working area 304, and/or to the second working area 306.
  • the contact dispenser 318 may carry one or more tips, e.g., to hold a volume of fluid.
  • the system 100 may include a mover 144, which in some aspects may be considered as part of the second working area 306.
  • the mover 144 may include a robotic arm and/or include grippers.
  • the stage 320 may carry the mover 144 and the contact dispenser 318 in some implementations.
  • the mover 144 may include a gantry having grippers that can pick-and-place objects such as the plates 120, 126 and/or the trays 132, 138 between different areas 102, 304, 306, 308 of the system 100, e.g., the mover 144 may move the first plate 126 from the consumables area 302 to the first plate receptacle 156.
  • the mover 144 may be implemented in different ways, however.
  • the stage 148 and the contact dispenser 148 may be movable to aspirate and/or dispense fluid between and above the consumables area 302 and the first working area 304 as a result.
  • the contact dispenser 148 may, for example, move linearly, which thereby reduces the risk of cross-contamination (between different samples) and allows some or all of the tips employed in the system 300 to be reusable for at least part of the processes performed by the system 300.
  • the stage 148 may be implemented differently, however.
  • the stage 148 may align the contact dispenser 145 with the tip tray 114 and the contact dispenser 145 couples with the first tip 116 from the tip tray 114. While the contact dispenser 145 is mentioned coupling with one first tip 116, the contact dispenser 145 may couple with a number of the first tips 116 that corresponds to the number of the wells 122 in the first plate 120 and/or the number of the wells 122 in the first plate 120 containing the sample 124.
  • the wells 122 may contain different samples 124, such as a biological sample derived from a human, animal, plant, bacteria, virus, or fungi.
  • the first tip 116 may be a smaller pipette tip that is used to move smaller fluid volumes and the second tip 118 may be a larger pipette tip that is used to move larger fluid volumes, for example.
  • Each of the first tip 116 and/or the second tip 118 may be exposed to a single sample during a workflow reducing the likelihood of cross-contamination and reducing the need to obtain a new tip after each operation. In some library preparation workflows, for example, each of the first tip 116 and/or the second tip 118 may be used through an entire workflow.
  • the contact dispenser 145 may couple with and/or use different ones of the tips 116, 118 depending on the workflow and/or the processes within a workflow that the system 100 is implementing.
  • the stage 320 may be an x-y-z stage, such that the mover 144 is movable in the x, y, and z directions.
  • the stage 320 and the contact dispenser 318 may be movable to aspirate and/or dispense fluid between and above the consumables area 302, the first working area 304, and/or the second working area 306 as a result.
  • the contact dispenser 148 may, for example, move linearly, which thereby reduces the risk of cross-contamination (between different samples) and allows some or all of the tips employed in the system 100 to be reusable for at least part of the processes performed by the system 100.
  • the stage 148 may be implemented differently, however.
  • the second working area 306 may include a light bar 155 that may be used to degrade oligonucleotides, such as a high power ultraviolet light (UV) light bar that is regularly used throughout a workflow to repeatedly degrade oligonucleotides to deter cross contamination in some implementations.
  • a light bar 155 that may be used to degrade oligonucleotides, such as a high power ultraviolet light (UV) light bar that is regularly used throughout a workflow to repeatedly degrade oligonucleotides to deter cross contamination in some implementations.
  • UV ultraviolet light
  • the first working area 304 may include a first plate receptacle 156 and a second plate receptacle 158
  • the second working area 306 may include a third plate receptacle 159, a fourth plate receptacle 160, and a fifth plate receptacle 161
  • the analyzer area 154 may include a substrate 162 and an imaging system 164, in at least some implementations.
  • the analyzer area 154 may use the substrate 162 that is implemented by a well plate in which a portion of the sample and a dye are dispensed
  • the imaging system 164 may image the portion of the sample in the well plate to determine a concentration of the sample.
  • the second working area 306 may include a reagent receptacle 250 having an access opening 252.
  • a reagent reservoir 254 is shown received within the reagent reservoir 306.
  • the first working area 304 may additionally or alternatively include a reagent receptacle 250 having an access opening 252.
  • the reagent receptacle 250 may be refrigerated and may be a drawer that can be pulled out from the system 100 and loaded with the reagent reservoir 254.
  • the reagent reservoir 254 may be accessed through the access opening 252 by the contact dispenser 318 to aspirate reagent from the reagent reservoir 254, for example.
  • the loading area 308 may be associated with loading and/or transferring a prepared sample to a system such as a sequencing system.
  • the first working area 304 may be associated with amplification processes and cleanup processes and the second working area 306 may be associated with quantification processes, library normalization processes, pooling processes, denaturing processes, and/or diluting processes.
  • the loading area 308 may include a sipper manifold assembly 174 in the implementation shown.
  • the sipper manifold assembly 174 may include sippers 184 to couple the sipper manifold assembly to a corresponding number of the flow cells.
  • the sipper manifold assembly 174 may include a valve 186 to control the flow of fluid through a fluidic line 188.
  • the sipper manifold assembly 174 may include a pump 187 to selectively flow the prepared sample from a well 128, 143 through the sipper 184, through the fluidic line 188, and out of the system 100, e.g., to another system used to perform an analysis on one or more samples of interest.
  • the valve 186 may be implemented by a rotary valve, a pinch valve, a flat valve, a solenoid valve, a check valve, a piezo valve, etc. Other fluid control devices may prove suitable.
  • the pump 187 may be implemented by a syringe pump, a peristaltic pump, and/or a diaphragm pump. Other types of fluid transfer devices may be used, however.
  • the controller 176 is electrically and/or communicatively coupled to components of the system 100 to perform various functions as disclosed herein.
  • the sipper manifold assembly 174 may alternatively be omitted.
  • the actuator 166 can move the magnet 150 between an upward position where the magnet 150 affects any plate positioned on the first plate receptacle 156 and a downward position where the magnet 150 does not affect any plate positioned on the first plate receptacle 156.
  • the magnet 150 being moved relative to the first plate receptacle 156 and any plate 120, 126, 142 positioned on the first plate receptacle 156 allows less area on the first working area 304 to be consumed.
  • the magnet 150 can moreover be moved with relatively higher confidence as compared to an alternative approach to moving one of the plates 120, 126, 142 filled with samples to a separate magnet station.
  • the magnet 150 may be implemented by a schach array configuration to strengthen and focus the corresponding magnetic fields.
  • the system 100 also includes a drive assembly 173.
  • the drive assembly 173 includes a pump drive assembly 219 and a valve drive assembly 220.
  • the pump drive assembly 219 may be adapted to interface with the pump 187 to pump fluid from the reagent reservoir 110 to the non-contact dispenser 146.
  • the valve drive assembly 220 may be adapted to interface with the valve 186 to control the position of the valve 186.
  • More or fewer instances of the various components of the system 100 shown in FIG. 1 may be included in the system 100 (e.g., four working areas 304, three consumable areas 310, etc.).
  • implementations “comprising,” “including,” or “having” an element or a plurality of elements having a particular property may include additional elements whether or not they have that property.
  • the terms “comprising,” including,” having,” or the like are interchangeably used herein.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Robotics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

La présente invention concerne un réseau de cartouches de réactif pour un système de préparation de banques comprenant une pluralité de cartouches de réactif, chaque cartouche de réactif de la pluralité de cartouches de réactif ayant un axe central et un grand axe de section transversale et un petit axe de section transversale, chaque cartouche de réactif de la pluralité de cartouches de réactif étant conçue pour être accessible par une pipette d'un système de préparation de banques. Chaque cartouche de réactif est espacée d'une cartouche de réactif adjacente du réseau dans une direction X et dans une direction Y, les espacements dans la direction X et la direction Y étant inégaux.
PCT/US2024/060632 2023-12-21 2024-12-18 Réseau à surface réduite de cartouches de réactif pré-aliquoté pour un système de préparation de banques Pending WO2025137016A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363613571P 2023-12-21 2023-12-21
US63/613,571 2023-12-21

Publications (1)

Publication Number Publication Date
WO2025137016A1 true WO2025137016A1 (fr) 2025-06-26

Family

ID=96137935

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/060632 Pending WO2025137016A1 (fr) 2023-12-21 2024-12-18 Réseau à surface réduite de cartouches de réactif pré-aliquoté pour un système de préparation de banques

Country Status (1)

Country Link
WO (1) WO2025137016A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001019502A2 (fr) * 1999-09-17 2001-03-22 Millipore Corporation Carte tamis de grande capacite
WO2013036941A2 (fr) * 2011-09-09 2013-03-14 Gen-Probe Incorporated Instrumentation de maniement automatisé d'échantillons, systèmes, processus et procédés associés
US20210183470A1 (en) * 2017-12-29 2021-06-17 Clear Labs, Inc. Automated priming and library loading device
CN117062670A (zh) * 2021-03-04 2023-11-14 中尺度技术有限责任公司 用于测定的包装和相关方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001019502A2 (fr) * 1999-09-17 2001-03-22 Millipore Corporation Carte tamis de grande capacite
WO2013036941A2 (fr) * 2011-09-09 2013-03-14 Gen-Probe Incorporated Instrumentation de maniement automatisé d'échantillons, systèmes, processus et procédés associés
US20210183470A1 (en) * 2017-12-29 2021-06-17 Clear Labs, Inc. Automated priming and library loading device
CN117062670A (zh) * 2021-03-04 2023-11-14 中尺度技术有限责任公司 用于测定的包装和相关方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ALLSHENG: "Library Preparation for NGS Practical Application Challenges | NGS Library Preparation Workstation Auto-NGS 100R|News & Events|Hangzhou Allsheng Instruments", ALLSHENG, 29 August 2022 (2022-08-29), pages 1 - 7, XP093326961, Retrieved from the Internet <URL:https://www.allsheng.com/news_cont_49.html> *

Similar Documents

Publication Publication Date Title
AU2023201695B2 (en) System and Method for Automated Single Cell Processing
CA2686597C (fr) Systeme et methode d&#39;extraction automatisee d&#39;acides nucleiques
US9329194B2 (en) Automated analyzer for clinical laboratory
US12188954B2 (en) Modules for transferring magnetic beads, automated system comprising the same and method for nucleic acid extraction using the same
KR101659619B1 (ko) 액체 샘플의 처리 시스템 및 처리 방법
US9079178B2 (en) Apparatus and methods for pipetting with interchangeability among different pipette tips
US20030129755A1 (en) System and method of storing and retrieving storage elements
US10788503B2 (en) Methods and apparatus for bead manipulation in a tip of a liquid handler
US9932574B2 (en) Suspension container for binding particles for the isolation of biological material
JP2013150623A (ja) 分析装置を含むシステムおよび方法
US20110207619A1 (en) Arrangement for processing a plurality of samples for analysis
KR102783214B1 (ko) 로봇 액체 취급 시스템용 열 순환기
US20130029856A1 (en) Transfer Or Interrogation Of Materials By Carrier And Receiving Devices Moving Independently And Simultaneously On Multiple Axes
WO2025137016A1 (fr) Réseau à surface réduite de cartouches de réactif pré-aliquoté pour un système de préparation de banques
EP4430409A1 (fr) Organisation automatisée de pointes de pipette pour systèmes de manipulation de fluide
US9746487B2 (en) Device for storing of fluid containers
EP2423688B1 (fr) Conteneur de suspension pour des particules de liaison pour l&#39;isolation de matériel biologique
WO2025136718A1 (fr) Appareil et procédés de réduction de l&#39;encombrement d&#39;une plateforme d&#39;automatisation de préparation de bibliothèque
US20250210142A1 (en) Method For Managing Multiple Parallel Library Preparation Workflows With Shared Resources
KR102787597B1 (ko) 로봇 핸들러를 사용하여 시약 저장조로부터 액체를 전달하는 방법
WO2025137085A1 (fr) Appareil de gestion d&#39;une contamination croisée dans une plateforme d&#39;automatisation de préparation de banque
WO2025137027A1 (fr) Système de vision pour un contrôle de qualité dans un système de préparation de bibliothèque
KR20250123671A (ko) 라이브러리 준비 시스템 및 연관된 방법
GB2579047A (en) Storage and feeding of disposable pipette tips
EP3197604A1 (fr) Distribution basée sur la pression

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24908785

Country of ref document: EP

Kind code of ref document: A1