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WO2025115320A1 - Dispositif de détection à des fins d'analyse d'émission de lumière, et automate d'analyse - Google Patents

Dispositif de détection à des fins d'analyse d'émission de lumière, et automate d'analyse Download PDF

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Publication number
WO2025115320A1
WO2025115320A1 PCT/JP2024/030343 JP2024030343W WO2025115320A1 WO 2025115320 A1 WO2025115320 A1 WO 2025115320A1 JP 2024030343 W JP2024030343 W JP 2024030343W WO 2025115320 A1 WO2025115320 A1 WO 2025115320A1
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WIPO (PCT)
Prior art keywords
detection device
base substrate
holding member
photodetector
claw portion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/JP2024/030343
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English (en)
Japanese (ja)
Inventor
豊 関根
禎昭 杉村
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Hitachi High Tech Corp
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Hitachi High Tech Corp
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Filing date
Publication date
Application filed by Hitachi High Tech Corp filed Critical Hitachi High Tech Corp
Publication of WO2025115320A1 publication Critical patent/WO2025115320A1/fr
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor

Definitions

  • This disclosure relates to a detection device for luminescence analysis and an automatic analysis device.
  • a technology has been disclosed for a luminescence analysis detection device and an automatic analyzer, in which a luminescent substrate is bound to the substance to be measured, a reaction liquid containing this substance is introduced into a flow cell, which is a measurement container, and the luminescence emitted when the substance is excited is detected by a photodetector (e.g., a photomultiplier tube), thereby quantitatively analyzing minute amounts of the substance to be measured (see Patent Document 1).
  • a photodetector e.g., a photomultiplier tube
  • a first member which is a member that holds the photodetector
  • a second member which is a member that has a measurement container
  • Fixing the first member and the second member with screws or the like in this way can suppress misalignment between the photodetector and the measurement container, thereby improving the light-blocking properties of the area surrounded by the flow path in the measurement container and the photodetector, and suppressing a decrease in the S/N ratio when measuring a signal with the photodetector.
  • the objective of this disclosure is to provide a technology that allows a second member having a measurement container to be attached and detached by simple means to a first member that holds a photodetector so that the photodetector and the measurement container are in an appropriate positional relationship.
  • an emission analysis detection device having a photodetector that detects light emitted from a sample to be measured, comprising: a cylindrical first member having an insertion hole that penetrates vertically and into which the photodetector is inserted; a base substrate provided vertically below the first member; a second member having a supply chamber into which the sample is supplied and disposed vertically below the base substrate; and a third member that abuts against the top surface of the first member around the insertion hole and has multiple claw portions that slide against the second member; and by rotating the third member while abutting against the top surface of the first member, the multiple claw portions are engaged with the second member, and the first member and the second member are fixed via the third member.
  • the present disclosure provides a technology that allows a second member having a measurement container to be attached and detached by simple means to a first member that holds a photodetector so that the photodetector and the measurement container are in an appropriate positional relationship.
  • FIG. 1 is a plan view illustrating an example of an automatic analyzer.
  • FIG. 2 is a front view showing an example of the emission analysis detection device according to the first embodiment.
  • FIG. 2 is a cross-sectional view showing an example of the emission analysis detection device according to the first embodiment.
  • FIG. 2 is a cross-sectional view showing an example of the emission analysis detection device according to the first embodiment.
  • 3A to 3C are diagrams for explaining the fixing principle in the emission analysis detection device of the first embodiment.
  • FIG. 4 is a front view showing a modification of the emission analysis detection device of the first embodiment.
  • 5A to 5C are diagrams illustrating a fixing operation of the emission analysis detection device according to the first embodiment.
  • FIG. 11 is a front view showing an example of an emission analysis detection device according to a second embodiment.
  • FIG. 6 is a cross-sectional view showing an example of an emission analysis detection device according to a second embodiment.
  • FIG. 11 is a cross-sectional view showing an example of an emission analysis detection device according to a third embodiment.
  • 11A to 11C are diagrams for explaining the immobilization principle in the emission analysis detection device of the third embodiment.
  • 13A to 13C are cross-sectional views illustrating a fixing operation of the emission analysis detection device according to the third embodiment.
  • the number of elements when referring to the number of elements (including the number, numerical value, amount, range, etc.), unless otherwise specified or clearly limited in principle to a specific number, the number is not limited to that specific number and may be more than or less than the specific number.
  • Luminescence analysis is a method used to analyze trace components. It is generally more sensitive than absorption measurement and is widely used in fields such as environmental analysis, pharmaceutical analysis, food analysis, clinical testing, and nucleic acid analysis. Luminescence here refers to the emission of light when an excited substance transitions to the ground state.
  • Luminescence is classified according to the cause of electron excitation. For example, luminescence produced when electrons are excited by heat is classified as thermoluminescence, luminescence produced when electrons are excited by light irradiation is classified as photoluminescence, and luminescence produced when electrons are excited by voltage is classified as electroluminescence.
  • the luminescence analysis detection device includes a measurement container that can be applied to luminescence analysis of these various luminescence modes. In each of the embodiments described below, an example is given of the application of the technology disclosed herein to luminescence analysis by chemiluminescence.
  • Chemiluminescence is also a form of luminescence, and is the phenomenon in which a substance produced by a chemical reaction emits light when it transitions from an excited state to a ground state.
  • Bioluminescence from fireflies, which promotes chemical reactions using enzymes, is a type of chemiluminescence.
  • a solution containing at least one chemiluminescent reagent is first supplied via a corresponding liquid delivery means, i.e., a sample, to a measurement container to cause a chemical reaction.
  • a photodetector using a photoelectric conversion element such as a photomultiplier tube or photodiode detects the light emitted from the solution by chemiluminescence in the measurement container.
  • the photoelectric conversion element then converts the light into a current intensity according to the amount of incident light, and the luminescence intensity is measured based on this.
  • the emission wavelength of chemical luminescence is generally in the visible light range (350 nm to 800 nm). Therefore, in each embodiment, the wavelength of the signal emission will be described as being visible light. However, even if the emission wavelength is outside the visible light range, the same effect can be obtained by selecting a material that matches the wavelength characteristics.
  • Automated analyzers are devices that analyze liquid samples derived from living organisms, such as blood and urine. In order to analyze liquid samples derived from living organisms with high sensitivity, it is essential to have a technology that can selectively recognize the target component of an analysis in a sample that contains a large amount of coexisting components.
  • the samples to be analyzed are antigens or antibodies in cancer markers, infectious diseases viruses, hormones, etc.
  • many protein components for example, about 70 g/L
  • the components to be analyzed are present in extremely small amounts on the order of f (femto) mol/L to n (nano) mol/L.
  • the analysis is carried out in the following steps (1) to (3).
  • a first antibody bound to magnetic particles and a second antibody bound to a luminescent label are mixed with an antigen to be analyzed, and the two are allowed to bind to each other through an antigen-antibody reaction.
  • the magnetic particles are magnetically captured using a permanent magnet, and coexisting components that are not bound to the magnetic particles are discharged outside the reaction vessel.
  • the luminescent labeling substance bound to the magnetic particles is caused to emit light, and the luminescence intensity, which depends on the concentration of the antigen to be analyzed, is measured.
  • Fig. 1 is a plan view showing a schematic diagram of an example of the overall configuration of an automatic analyzer. First, the overall configuration of an automatic analyzer equipped with an emission analysis detection device will be described with reference to Fig. 1.
  • the automatic analysis device 100 comprises an analysis unit 110, a control unit 130, an input unit 150, and a display device 170.
  • the analysis unit 110 performs the analysis operation.
  • the control unit 130 controls the entire device.
  • the input unit 150 accepts information input by the user.
  • the display device 170 displays various information.
  • the input unit 150 may also be used as the display device 170, one example of which is a touch panel monitor.
  • the analysis section 110 includes a transport mechanism 111, a sample probe 112, a chip loading/unloading section 113, a chip magazine 114, a reaction vessel magazine 115, a chip/reaction vessel transport mechanism 116, an incubator (reaction disk) 117, a reagent disk 118, a reagent dispensing probe 119, a reagent probe washing section 120, a magnetic particle stirring mechanism 121, a magnetic particle stirring mechanism washing section 122, a dispensing probe for detection device 123, and a detection device for luminescence analysis (hereinafter also simply referred to as the detection device) 200.
  • a detection device for luminescence analysis hereinafter also simply referred to as the detection device 200.
  • the transport mechanism 111 is a device that transports a sample container 124 containing a sample to a sample dispensing position.
  • the sample probe 112 is a device that dispenses the sample.
  • the tip attachment/detachment unit 113 is a device that attaches and detaches a disposable tip (hereinafter, tip) to and from the sample probe 112.
  • the chip magazine 114 is a device that supplies chips.
  • the reaction vessel magazine 115 is a device that supplies reaction vessels.
  • the chip/reaction vessel transport mechanism 116 is a device that transports chips and reaction vessels.
  • the incubator 117 is a device equipped with multiple openings 125 that can maintain the reaction liquid in the reaction vessel at a constant temperature.
  • the reagent disk 118 is a device that holds the reagent vessel 126 that contains the analytical reagent.
  • the reagent dispensing probe 119 is a device that dispenses analytical reagents into the incubator 117.
  • the reagent probe washing unit 120 is a device that washes the reagent dispensing probe 119 with water or a washing solution.
  • the magnetic particle stirring mechanism 121 is a device that stirs the analytical reagent containing magnetic particles before dispensing.
  • the magnetic particle stirring mechanism cleaning unit 122 is a device that cleans the magnetic particle stirring mechanism 121 with water or a cleaning solution.
  • the detection device 200 is a device that performs luminescence detection.
  • the detection device dispensing probe 123 is a device that dispenses reaction liquid into the detection device 200.
  • Multiple bottles, including spare bottles, are stored in the automatic analyzer 100 to supply common reagents such as luminescence reagents, cleaning liquid, and probe cleaning liquid, and each reagent is supplied to the corresponding mechanism through a reagent tube inserted into each bottle.
  • reaction reagent a measurement reagent (reaction reagent) containing magnetic particles and a measurement reagent (reaction reagent) containing a first antibody are dispensed and mixed into the reaction vessel by the reagent dispensing probe 119, and incubation is performed for a certain period of time.
  • the sample container 124 containing the sample is transported to the sample collection position by the transport mechanism 111, a tip is attached to the sample probe 112 in the tip attachment/detachment section 113, and the sample is dispensed by the sample probe 112 into a reaction container above the incubator 117.
  • a measurement reagent containing a second antibody is dispensed into the reaction container by the reagent dispensing probe 119, and incubation is performed for a fixed period of time.
  • the detection device (luminescence analysis detection device) 200 is used, for example, in fields such as clinical testing, nucleic acid analysis, pharmaceutical analysis, food analysis, and environmental analysis.
  • the photodetector holding member 220 is a member for holding the photodetector 210, and has an insertion hole 221, which is a space into which the photodetector 210 is inserted. That is, the photodetector holding member 220 is a cylindrical member in which the insertion hole 221 is formed.
  • the insertion hole 221 is a space whose opening shape is approximately circular, and is provided penetrating the photodetector holding member 220 along the longitudinal direction of the photodetector holding member 220.
  • the photodetector holding member 220 is arranged so that its longitudinal direction is along the vertical direction (Z direction in the figure).
  • the lower end of the photodetector holding member 220 (the lower end in the Z direction in FIG. 2) is provided with a large diameter portion 222 whose diameter (outer diameter) is larger than the other portions of the photodetector holding member 220.
  • the photodetector holding member 220 is positioned and fixed to the base substrate 230 with this large diameter portion 222 abutting against the base substrate 230.
  • the base substrate 230 is a substrate on which the photodetector holding member 220 and the measurement container holding member 250 are positioned and fixed, and is disposed between the photodetector holding member 220 and the measurement container holding member 250.
  • the base substrate 230 has a communication hole 231 formed therein, which has approximately the same opening diameter as the insertion hole 221 of the light detector holding member 220 and communicates with the insertion hole 221.
  • the light detector holding member 220 and the base substrate 230 are positioned and fixed so that the communication hole 231 overlaps with the insertion hole 221 in the Z direction.
  • the light detector 210 is held by the light detector holding member 220 in a state where it is inserted into the communication hole 231 together with the insertion hole 221. It can be said that the insertion hole 221 and the communication hole 231 constitute a holding portion that holds the light detector 210.
  • the photodetector 210 is inserted into the insertion hole 221 and the communication hole 231, and the periphery of the photodetector 210 is covered by the photodetector holding member 220 and the base substrate 230, thereby preventing external light unnecessary for measurement from entering the photodetector 210 and becoming noise light.
  • the photodetector holding member 220 is matte-finished or black-painted, or both. This makes it possible to more efficiently attenuate unnecessary external light (stray light) that strikes the photodetector 210.
  • the base substrate 230 is similarly treated.
  • the photodetector holding member 220 can also be used to mechanically connect the emission analysis detection device 200 to an external analytical device (not shown).
  • the photodetector holding member 220 and the base substrate 230 are configured as separate members, but the photodetector holding member 220 and the base substrate 230 may be formed integrally.
  • the base substrate 230 may constitute a part of the photodetector holding member 220, which is the first member.
  • the measurement container 240 is, for example, a disk-shaped member, and is formed with a supply chamber 241 into which the sample to be measured is supplied. This supply chamber 241 is also the part that causes the supplied sample to emit light.
  • the measurement container 240 is arranged vertically below the base substrate 230 while being held by a measurement container holding member 250.
  • the measurement container 240 in this embodiment is formed with a diameter of about 30 mm, and a sample is placed in the supply chamber 241 when performing emission analysis.
  • the measurement container holding member 250 is a member that holds the measurement container 240, and has a storage section 251 which is a space in which the measurement container 240 is stored.
  • the measurement container holding member 250 is positioned and fixed to the base substrate 230 with the measurement container 240 stored in the storage section 251.
  • the measurement container 240 is also disposed in a region facing the communication hole 231 of the base substrate 230 in the vertical direction (Z direction in the figure). In other words, the measurement container 240 is disposed so as to face the photodetector 210 held by the photodetector holding member 220 in the Z direction.
  • the configuration of the measurement container 240 and the measurement container holding member 250 is not particularly limited.
  • the measurement container holding member 250 may also serve as the measurement container 240.
  • a flow path that functions as the supply chamber 241 may be formed in the measurement container holding member 250, and the sample may be continuously supplied to this flow path.
  • Examples of components formed by the measurement container 240 and the measurement container holding member 250 include flow cells (flow-through cells) used in immunological analysis in clinical tests, and nucleic acid microarrays (DNA chips) used in nucleic acid analysis.
  • flow cells flow-through cells
  • DNA chips nucleic acid microarrays
  • the measurement container holding member 250 can also be used to mechanically connect this emission analysis detection device to an external analysis device (not shown).
  • One role of the measurement container holding member 250 is, for example, to facilitate temperature control by thermally connecting the measurement container 240 to an external temperature control device (not shown) in order to perform highly accurate analysis.
  • the "side surface" and "at least a part of the outer periphery of the top surface” of the measurement container 240 are covered by the measurement container holding member 250 as shown in FIG. 3.
  • the outer periphery of the top surface of the measurement container 240 means the outer periphery of the top surface, i.e., the surface facing the photodetector 210.
  • Another purpose of covering part of the measurement container 240 with the measurement container holding member 250 is to protect the measurement container 240.
  • Still another purpose is to prevent external light unnecessary for measurement from entering the photodetector 210 through the measurement container 240 and becoming noise light.
  • the measurement container holding member 250 is preferably matte-finished and black-painted to efficiently attenuate unwanted external light emission.
  • the measurement container holding member 250 is made of an aluminum material and is matte-finished and black-painted.
  • the fixing member 260 is a member for fixing the measurement container holding member 250 in a positioned state relative to the base substrate 230. Furthermore, in this embodiment, the fixing member 260 fixes the photodetector holding member 220 in a positioned state relative to the base substrate 230. In other words, the fixing member 260 is a member for fixing the photodetector holding member 220 and the base substrate 230 to the measurement container holding member 250 in a positioned state.
  • the measurement container holding member 250 is provided with cylindrical protrusions 252 as protrusions that protrude from the upper surface of the measurement container holding member 250 toward the photodetector holding member 220.
  • the cylindrical protrusions 252 are provided so as to protrude upward in the vertical direction.
  • the cylindrical protrusions 252 are provided at two locations that are point symmetrical with respect to the center of the measurement container 240 (the center of the storage section 251). These two cylindrical protrusions 252 are provided at intervals of 180 degrees around the storage section 251 of the measurement container holding member 250.
  • Each cylindrical protrusion 252 fits into a fitting hole 232 provided in the base substrate 230. That is, the base substrate 230 is provided with a plurality of fitting holes 232 (two in this embodiment) corresponding to each cylindrical protrusion 252.
  • Each cylindrical protrusion 252 has a length (height) that protrudes to the upper surface side of the base substrate 230, i.e., the photodetector holding member 220 side.
  • a groove portion 253 is provided on at least a part of the outer periphery of each cylindrical protrusion 252 on the upper surface side of the base substrate 230. This groove portion 253 extends in the circumferential direction of the cylindrical protrusion portion 252. In this embodiment, the groove portion 253 is formed around the entire circumference of the cylindrical protrusion 252.
  • the photodetector holding member 220 and the base substrate 230 may be provided with a fitting structure for positioning them.
  • one of the photodetector holding member 220 or the base substrate 230 may be provided with a convex portion that protrudes toward the other, and the other may be provided with a concave portion into which the convex portion fits.
  • the photodetector holding member 220 and the base substrate 230 may be positioned and fixed in advance by a means other than the fixing member 260.
  • the cylindrical protrusion 252 of the measurement container holding member 250 and the fitting hole 232 of the base substrate 230 are formed with an arbitrary fitting tolerance that matches the positioning accuracy required for the measurement container holding member 250.
  • the measurement container holding member 250 is positioned relative to the base substrate 230 by fitting the cylindrical protrusion 252 into the fitting hole 232.
  • the measurement container holding member 250 holding the measurement container 240 can be installed at a position with good reproducibility relative to the base substrate 230.
  • the measurement container holding member 250 holding the measurement container 240 can be positioned with high precision relative to the base substrate 230.
  • cylindrical protrusions 252 are configured as separate members that can be separated from the measurement container holding member 250.
  • each cylindrical protrusion 252 and the measurement container holding member 250 are fixed to each other by a female screw provided on the measurement container holding member 250 and a male screw provided on one end of the cylindrical protrusion 252.
  • the material of the cylindrical protrusions 252 can be any material as long as it is not a material with an extremely low elastic modulus such as rubber.
  • stainless steel is used as the material of the cylindrical protrusions 252, but resins such as polyphenylene sulfide (PPS), polyether ether ketone (PEEK), and polyamide (PA) may also be used.
  • PPS polyphenylene sulfide
  • PEEK polyether ether ketone
  • PA polyamide
  • the cylindrical protrusion 252 may be provided so as to protrude from the upper surface of the measurement container holding member 250 to the upper surface side of the base substrate 230, and its configuration is not particularly limited.
  • the cylindrical protrusion 252 may be fastened to the measurement container holding member 250 by, for example, press fitting, shrink fitting, etc., or may be formed integrally with the measurement container holding member 250 by, for example, cutting, molding, or other means.
  • the cylindrical protrusion 252 may have a conical or spherical tip so that it can be easily inserted into the fitting hole 232 of the base substrate 230.
  • the outer circumferential surface of the cylindrical protrusion 252 may be coated with a sliding material such as a fluorine-based resin.
  • part of the outer periphery of the cylindrical protrusion 252 is machined to have a shape such as a D-cut, a slot, or a cross groove. This makes it easier to attach the cylindrical protrusion 252 to the measurement container holding member 250.
  • the fixing member 260 is a member having a substantially circular planar shape, and has an insertion hole 261 formed in its center, through which the photodetector holding member 220 is inserted.
  • This insertion hole 261 is formed with an inner diameter smaller than the outer diameter of the large diameter portion 222 of the photodetector holding member 220. Therefore, when the photodetector holding member 220 is inserted into the insertion hole 261, the lower surface of the fixing member 260 abuts against the upper surface of the large diameter portion 222.
  • the fixing member 260 is formed so that it can be rotated in this state.
  • the fixing member 260 has a ring portion 262 that is rotatably arranged around the outer periphery of the photodetector holding member 220.
  • the fixing member 260 also has a claw portion 263 that protrudes horizontally from the outer periphery of the ring portion 262.
  • the claw portion 263 is formed with a thickness thinner than the thickness of the ring portion 262.
  • the claw portion 263 is provided corresponding to each cylindrical protrusion 252 so that it fits into the groove portion 253 of each cylindrical protrusion 252 by rotating the fixing member 260 (ring portion 262). That is, the claw portion 263, like the cylindrical protrusion 252, is arranged in two locations that are point symmetrical with respect to the center of the measurement container 240.
  • a wall portion 264 is provided on part of the outer periphery of the ring portion 262, protruding from the underside of the ring portion 262 towards the base substrate 230.
  • the fixing member 260 according to this embodiment is provided at two locations that are point symmetrical with respect to the center of the measurement container 240.
  • the two wall portions 264 are provided continuously over a range of 90 degrees along the outer periphery of the ring portion 262. In other words, the two wall portions 264 are provided at an interval of 90 degrees on the outer periphery of the ring portion 262.
  • the claw portion 263 is provided on the lower end surface 264a of this wall portion 264.
  • the two claw portions 263 are provided continuously over a range of 90 degrees along the outer periphery of the ring portion 262.
  • each wall portion 264 is a helical surface with the center of the insertion hole 261 as its axis.
  • the claw portion 263 is arranged on a helical surface provided on the wall portion 264 protruding from the ring portion 262 with the center of the insertion hole 261 as its axis.
  • the claw portion 263 is provided so that the upper surface 263a is a helical surface.
  • a helical surface refers to a surface that is curved in an arc and whose height changes continuously.
  • the upper surface 263a of the claw portion 263 may also be referred to as the helical surface 263a.
  • the upper surfaces 263a of the two claw portions 263 do not form a continuous spiral surface, and as shown in FIG. 2, the two claw portions 263 must be provided in symmetrical directions. In other words, the two claw portions 263 must be positioned so that when the fixing member 260 is rotated 180 degrees, one claw portion 263 is in the position of the other claw portion 263.
  • the upper surface (helical surface) 263a of the claw portion 263 is a surface formed by continuously changing a line segment that is horizontal in any vertical cross section as shown in FIG. 3 along the helical winding, but in practical use, the helical surface 263a may be a surface approximated by a circular arc or a spline.
  • the side surface (upper surface) 253a of the groove 253 of the cylindrical protrusion 252 with which the upper surface (helical surface) 263a of the claw portion 263 comes into contact is a horizontal surface, but in the configuration of this embodiment, since the inclination of the helical surface 263a is slight, the claw portion 263 and the cylindrical protrusion 252 deform within the elastic range of the material, and the two come into contact almost parallel.
  • the upper surface 263a of the claw portion 263 is a helical surface
  • the claw portion 263 and the cylindrical protrusion 252 slide against each other as the fixing member 260 rotates, and the contact position between the claw portion 263 and the cylindrical protrusion 252 moves vertically upward.
  • the measurement container holding member 250 holding the measurement container 240 is pushed up vertically.
  • the measurement container holding member 250 is pressed against the lower surface of the base substrate 230. This positions and fixes the measurement container holding member 250 to the base substrate 230.
  • the fixing member 260 rotates, the fixing member 260 is pressed against the upper surface of the large diameter portion 222 of the photodetector holding member 220. As a result, the photodetector holding member 220 is pressed against the upper surface of the base substrate 230. This positions and fixes the photodetector holding member 220 to the base substrate 230. Furthermore, the photodetector holding member 220 and the measurement container holding member 250, which are arranged on either side of the base substrate 230, are fixed via the fixing member 260.
  • Figure 5 is a diagram explaining the fixing principle in the detection device, and is a schematic diagram that enlarges the contact portion between the claw portion 263 and the cylindrical protrusion 252 in Figure 3 and emphasizes the lead angle ⁇ of the helical surface 263a for explanation purposes.
  • Figure 6 is a schematic diagram for explaining the fixing operation of the detection device.
  • the cylindrical projection 252 receives a load component N perpendicular to the helical surface 263a and a frictional force ⁇ N along the helical surface 263a due to the torque.
  • the load N is a reaction force generated when the wall portion 264 (ring portion 262) on which the claw portion 263 is provided and the cylindrical projection 252 stretch as the contact portion 270 between the claw portion 263 and the cylindrical projection 252 moves vertically upward as the fixed member 260 rotates.
  • the relationship between the rotation angle and the load N when the fixed member 260 is rotated with the claw portion 263 and the cylindrical projection 252 in contact with each other is determined by the elastic modulus of the material of the fixed member 260 and the cylindrical projection 252.
  • the load N is smaller when the material with a low elastic modulus is used at the same rotation angle of the fixing member 260.
  • the resultant force Ncos ⁇ + ⁇ Nsin ⁇ of these vertical components acts as the vertical load F on the cylindrical protrusion 252, which becomes the fastening force of the measurement container holding member 250. Therefore, for a given load N, the smaller the lead angle ⁇ of the helical surface 263a, the greater the vertical load F. As a result, the fastening force between the cylindrical protrusion 252 and the claw portion 263 increases, improving the light blocking properties of the photodetector 210 when the measurement container holding member 250 is fixed to the base substrate 230.
  • the outer diameter of the helical surface 263a is small, in order to avoid excessively increasing the amount of rotation of the fixing member 260 required to fix the measurement container holding member 250 to the base substrate 230, it is necessary to increase the lead angle ⁇ .
  • the upper surface (helical surface) 263a of the claw portion 263 and the inner surface (upper surface) of the groove portion 253 deviate from a nearly parallel state. This may result in the claw portion 263 being unable to slide against the inner surface of the groove portion 253 of the cylindrical protrusion 252.
  • the lead of the helical surface 263a it is desirable to determine the lead of the helical surface 263a taking these points into consideration. For example, it is desirable to determine the lead of the helical surface 263a so that the amount of rotation of the fixing member 260 (ring portion 262) required to fix the measurement container holding member 250 to the base substrate 230 is within a range that is not troublesome in practical use. As an example, it is preferable to set the outer diameter of the helical surface 263a to 65 mm, the lead to approximately 4 mm, and the rotation range of the fixing member 260 required to fix the measurement container holding member 250 to the base substrate 230 to less than 90 degrees. In this case, the lead angle ⁇ is 1.1 degrees, and the height difference between both ends of the helical surface 263a in the longitudinal direction is approximately 1 mm.
  • the fixed state of the photodetector holding member 220, base substrate 230, and measurement container holding member 250 is maintained by the frictional force at the contact portion 270 between the cylindrical protrusion 252 and the claw portion 263 when the fixing member 260 is rotated, but the means for maintaining the fixed state is not limited to this.
  • a knob-equipped set screw may be provided on the outer periphery of the ring portion 262 to maintain the fixed state between the fixing member 260 and the photodetector holding member 220.
  • a ball plunger may be provided on one of the ring portion 262 and the photodetector holding member 220, and a hole into which the ball fits may be provided on the other member. This holds the fixing member 260 at a specific angle, and maintains the fixed state between the fixing member 260 and the photodetector holding member 220. As a result, the fixed state between the measurement container holding member 250 and the base substrate 230 is also maintained.
  • the claw portion 263 is provided on the lower end surface 264a of the wall portion 264. Therefore, the dimensional error of the cylindrical protrusion 252 can be absorbed by the amount of rotation of the fixing member 260. In other words, even if there is a dimensional error in the cylindrical protrusion 252, the fixing member 260 can appropriately fix the measurement container holding member 250 etc. with good reproducibility.
  • the rotation angle (ring angle) from when the fixing member 260 begins to rotate right to when the claw portion 263 begins to contact the groove portion 253 of the cylindrical protrusion 252 is larger than in the example of FIG. 2.
  • the fixing member 260 can be tightened with the desired torque within the movable range of the ring portion 262, that is, within the range in the circumferential direction of the ring portion 262 where the claw portion 263 is provided, regardless of the ring angle at which the two begin to contact.
  • the measurement container holding member 250 and the like can be fixed with the desired fastening force, and therefore reproducibility is good.
  • the measurement container holding member 250 and the like can be positioned and fixed with high precision to the base substrate 230.
  • the fixing member 260 can be manufactured by, for example, a cutting method, a molding method, a three-dimensional modeling method, or the like. In addition, in the case of the fixing member 260 according to this embodiment, it can also be manufactured by a cutting method using a multi-tasking machine.
  • the claw portion 263 and the ring portion 262 (wall portion 264) that constitute the fixing member 260 are separate and separate members that are fastened together using screws, but they may also be integrated.
  • the claw portion 263 and the ring portion 262 may be manufactured as an integrated unit by cutting, molding, welding, three-dimensional modeling, or other methods.
  • the ring portion 262 and the claw portion 263 are separate, detachable members or integrated members. Because the claw portion 263 is a member that slides against the cylindrical projection 252, making it a separate, detachable member from the ring portion 262 has the advantage that it can be locally replaced in response to wear and other deterioration that occurs with use.
  • the claw portion 263 is a component that receives a concentrated load from the cylindrical projection 252, it is necessary to consider the risk of destruction. However, when the claw portion 263 is integrated with the ring portion 262, the risk of destruction of the claw portion 263 is kept low.
  • the fixing member 260 (ring portion 262) can be rotated by hand by an operator without using tools.
  • the fixing member 260 can be formed into any shape as long as it rotates around the outer periphery of the photodetector holding member 220 and has a claw portion 263 that protrudes horizontally and fits into the groove portion 253 of the cylindrical protrusion 252.
  • the fixing member 260 may include a handle 265 provided on the upper surface of the ring portion 262, as shown in FIG. 6, for example.
  • the fixing member 260 may be manufactured so that the shape of the ring portion 262 itself functions as a handle. This can improve the operability of the fixing member 260 by the operator.
  • the materials of the ring portion 262 and the claw portion 263 constituting the fixing member 260 can be selected arbitrarily, provided they are not extremely low in elasticity, such as rubber.
  • the ring portion 262 is made of polyamide
  • the claw portion 263 is made of stainless steel.
  • Polyamide is known to have relatively high tensile strength, elasticity, and abrasion resistance among resin materials, and is also self-lubricating, making it a suitable example of a material for the ring portion 262 that rotates around the photodetector holding member 220 and receives a fastening force from the cylindrical projection 252.
  • Stainless steel has excellent mechanical strength, so it is difficult to deform even when a thin-walled shape such as the claw portion 263 receives a concentrated load from the cylindrical projection 252, and has excellent abrasion resistance, making it a suitable example of a material for the claw portion 263.
  • reference marks, scales, etc. may be provided on the outer periphery of the ring portion 262 of the base substrate 230 or the fixing member 260 so that the rotation angle of the fixing member 260 (ring portion 262) can be quantitatively identified, or so that it can be identified whether the measurement container holding member 250, etc. is properly fixed to the base substrate 230.
  • a sliding coating, sliding tape, washer, bearing, etc. may be provided on the contact parts between the ring portion 262 and the photodetector holding member 220, such as the upper surface of the large diameter portion 222, the lower surface of the ring portion 262, and the inner surface of the insertion hole 261.
  • Figs. 7(a) to (c) are perspective views for explaining the fixing operation of the measurement container holding member and the like by the fixing member. Note that in Fig. 7, in order to easily show the main parts, the base substrate 230 and members that are not important for the explanation are omitted.
  • FIG. 7(a) is a diagram showing the state in which the photodetector holding member 220, base substrate 230, measurement container holding member 250, and fixing member 260 are assembled and the fixing member 260 is not rotated.
  • FIG. 7(b) is a diagram showing the state in which the fixing member 260 is rotated until the cylindrical protrusion 252 and the claw portion 263 start to come into contact.
  • FIG. 7(c) is a diagram showing the state in which the photodetector holding member 220, base substrate 230, and measurement container holding member 250 are fixed by the fixing member 260 by further rotating the fixing member 260 with an arbitrary torque after the cylindrical protrusion 252 and the claw portion 263 come into contact.
  • the photodetector holding member 220, base substrate 230, measurement container holding member 250, and fixing member 260 are assembled.
  • the cylindrical projection 252 of the measurement container holding member 250 is fitted into the fitting hole 232 of the base substrate 230 (see FIG. 3).
  • the fixing member 260 (ring portion 262) is also arranged around the photodetector holding member 220 arranged on the base substrate 230. That is, the photodetector holding member 220 is inserted into the insertion hole 261 of the fixing member 260.
  • the cylindrical projection 252 is arranged between the two wall portions 264 so that the claw portion 263 does not interfere with the cylindrical projection 252.
  • the fixing member 260 is rotated counterclockwise. This causes the claw portion 263 to enter the groove portion 253 of the cylindrical protrusion 252.
  • the upper surface 263a of the claw portion 263 is a helical surface, so that as the fixing member 260 rotates, the gap between the side surface (upper surface) of the groove portion 253 and the upper surface (helical surface) 263a of the claw portion 263 gradually becomes smaller, and the claw portion 263 and the cylindrical protrusion 252 begin to come into contact.
  • the fixing member 260 of embodiment 2 does not have a wall portion, and the claw portion 263 is provided so as to protrude from the lower end of the outer diameter surface of the ring portion 262 toward the outside of the ring portion 262.
  • the upper surface 263a of the claw portion 263 is machined into a spiral surface (helical surface).
  • the claw portion 263 is formed so that its thickness gradually increases in the circumferential direction of the ring portion 262, and as a result, the upper surface 263a of the claw portion 263 is a helical surface.
  • the claw portion 263 can be manufactured by, for example, cutting, molding, three-dimensional modeling, etc. In the case of the fixing member 260 of this embodiment, it can also be manufactured by a cutting method using a multi-tasking machine. Furthermore, the cylindrical protrusion 252 provided on the measurement container holding member 250 is formed to a predetermined length according to the position of the claw portion 263 in the Z direction. In the example of FIG. 8, the position of the claw portion 263 is farther away from the base substrate 230 in the Z direction than in the example of embodiment 1. Therefore, the length of the cylindrical protrusion 252 is longer than that of embodiment 1, and a groove portion 253 is formed at a position corresponding to the claw portion 263.
  • the upper surface (helical surface) 263a of the claw portion 263 of the fixing member 260 comes into contact with the side surface (upper surface) of the groove portion 253 provided in the cylindrical protrusion 252, thereby fixing the measurement container holding member 250, etc. to the base substrate 230.
  • the tip surface 263b of the claw portion 263 of the fixing member 260 comes into contact with the bottom surface 253b of the groove portion 253 provided in the cylindrical protrusion 252, thereby fixing the measurement container holding member 250, etc. to the base substrate 230.
  • the cylindrical projection 252 is disposed at a first position P1 near the end of the wall portion 264 (the end on the forward side in the rotation direction of the fixing member).
  • the tip surface 263b of the claw portion 263 and the bottom surface 253b of the groove portion 253 of the cylindrical projection 252 are separated.
  • the claw portion 263 and the cylindrical projection 252 are disposed with a gap therebetween.
  • the cylindrical protrusion 252 moves relatively to the second position P2, as shown in FIG. 11.
  • the inner diameter D1 of the claw portion 263 gradually decreases, that is, the position of the tip surface 263b of the claw portion 263 moves horizontally. Therefore, the gap between the claw portion 263 and the cylindrical protrusion 252 narrows.
  • the bottom surface 253b of the groove portion 253 of the cylindrical protrusion 252 comes into contact with the tip surface 263b of the claw portion 263.
  • the photodetector holding member 220, the base substrate 230, and the measurement container holding member 250 can be fixed in positions with good reproducibility by simple means that do not require tools for attachment and detachment.
  • the detection device 200 can fix the measurement container in a reproducible position using simple means that does not require tools for installation and removal, so that the measurement container 240 can be replaced quickly, and the good reproducibility of the installation position of the measurement container 240 contributes to reducing variability in the analysis results.
  • the planar projection of tip surface 263b of claw portion 263 is preferably a curve whose radius is defined as a function of angle as described above, but is not limited to this.
  • Tip surface 263b of claw portion 263 needs only to be formed into a curved surface that allows claw portion 263 to slide within groove portion 253 when fixing member 260 is rotated, generating a load that pulls cylindrical projection 252 up in the vertical direction.
  • the tip surface 263b of the claw portion 263 and the bottom surface 253b of the groove portion 253 are inclined surfaces inclined at a predetermined angle ⁇ 1, but the configuration of both is not limited to this.
  • the inclination angles of the tip surface 263b of the claw portion 263 and the bottom surface 253b of the groove portion 253 do not necessarily have to be the same.
  • either the tip surface 263b of the claw portion 263 or the bottom surface 253b of the groove portion 253 may be an inclined surface. Even in this case, the photodetector holding member 220 and the base substrate 230 can be fixed to the measurement container holding member 250.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Est proposée une technologie dans laquelle, à l'aide d'un moyen simple, un deuxième élément qui est pourvu d'un récipient de mesure peut être fixé à un premier élément qui retient un photodétecteur et détaché de celui-ci, de sorte que le photodétecteur et le récipient de mesure présentent une relation de position appropriée. Un dispositif de détection (200) à des fins d'analyse d'émission de lumière comprend : un premier élément cylindrique (220) qui possède un détecteur de lumière (210) pour détecter de la lumière émise par un échantillon en cours de mesure, et qui possède un orifice d'insertion (221) prévu pénétrant dans un sens vertical et dans lequel le détecteur de lumière (210) est inséré ; un substrat de base (230) qui est disposé sur le côté inférieur du premier élément (220) dans le sens vertical ; un deuxième élément (250) qui possède une chambre d'alimentation dans laquelle l'échantillon est alimenté, et qui est positionné sur le côté inférieur du substrat de base (230) dans le sens vertical ; et un troisième élément (260) qui vient en contact avec une surface supérieure du premier élément (220) à la périphérie de l'orifice d'insertion (221), et qui possède une pluralité de sections de languette (263) qui coulissent par rapport au deuxième élément (250), par rotation du troisième élément (260) tandis que celui-ci est en contact avec le côté surface supérieure du premier élément (220), les sections de languette de la pluralité de sections de languette (263) étant verrouillées par le deuxième élément (250), le premier élément 220 et le deuxième élément (250) étant fixés par l'intermédiaire du troisième élément (260).
PCT/JP2024/030343 2023-11-27 2024-08-26 Dispositif de détection à des fins d'analyse d'émission de lumière, et automate d'analyse Pending WO2025115320A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2023199552 2023-11-27
JP2023-199552 2023-11-27

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WO2025115320A1 true WO2025115320A1 (fr) 2025-06-05

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PCT/JP2024/030343 Pending WO2025115320A1 (fr) 2023-11-27 2024-08-26 Dispositif de détection à des fins d'analyse d'émission de lumière, et automate d'analyse

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0416237Y2 (fr) * 1986-08-08 1992-04-10
WO2011101913A1 (fr) * 2010-02-19 2011-08-25 三菱電機株式会社 Pièce de fixation permettant de monter un module de type capteur ultrasonore et procédé de fixation
JP2018084547A (ja) * 2016-11-25 2018-05-31 日本電産トーソク株式会社 油圧センサ取付構造
JP2021120621A (ja) * 2020-01-30 2021-08-19 株式会社日立ハイテク フローセルおよび自動分析装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0416237Y2 (fr) * 1986-08-08 1992-04-10
WO2011101913A1 (fr) * 2010-02-19 2011-08-25 三菱電機株式会社 Pièce de fixation permettant de monter un module de type capteur ultrasonore et procédé de fixation
JP2018084547A (ja) * 2016-11-25 2018-05-31 日本電産トーソク株式会社 油圧センサ取付構造
JP2021120621A (ja) * 2020-01-30 2021-08-19 株式会社日立ハイテク フローセルおよび自動分析装置

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