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WO2025193197A1 - Système à base de lévitation magnétique pour la mesure simultanée de la susceptibilité magnétique et de la densité de microparticules et de cellules - Google Patents

Système à base de lévitation magnétique pour la mesure simultanée de la susceptibilité magnétique et de la densité de microparticules et de cellules

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Publication number
WO2025193197A1
WO2025193197A1 PCT/TR2025/050107 TR2025050107W WO2025193197A1 WO 2025193197 A1 WO2025193197 A1 WO 2025193197A1 TR 2025050107 W TR2025050107 W TR 2025050107W WO 2025193197 A1 WO2025193197 A1 WO 2025193197A1
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WO
WIPO (PCT)
Prior art keywords
magnetic
microparticles
magnetic levitation
levitation
density
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/TR2025/050107
Other languages
English (en)
Inventor
Hüseyin Cumhur TEKİN
Betül KARAKUZU
Original Assignee
İzmi̇r Yüksek Teknoloji̇ Ensti̇tüsü Rektörlüğü
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from TR2024/002998 external-priority patent/TR2024002998A1/tr
Application filed by İzmi̇r Yüksek Teknoloji̇ Ensti̇tüsü Rektörlüğü filed Critical İzmi̇r Yüksek Teknoloji̇ Ensti̇tüsü Rektörlüğü
Publication of WO2025193197A1 publication Critical patent/WO2025193197A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/32—Magnetic separation acting on the medium containing the substance being separated, e.g. magneto-gravimetric-, magnetohydrostatic-, or magnetohydrodynamic separation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0626—Fluid handling related problems using levitated droplets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces

Definitions

  • the invention relates to a magnetic levitation-based system used for the simultaneous measurement of the magnetic susceptibility and density of microparticles and cells.
  • a magnetic levitation-based system for use in the simultaneous measurement of the magnetic susceptibility and density of microparticles and cells.
  • the magnetic levitation-based system proposed to the invention comprises two neodymium (N52) magnets with same poles facing each other, and a microfluidic chip consisting of a glass capillary or a polymethylmethacrylate (PMMA) plate, a glass slide, and double-sided adhesive placed between the magnets.
  • N52 neodymium
  • PMMA polymethylmethacrylate
  • Equation 1 the magnetic susceptibility and density values for the particle/cell are calculated.
  • the magnetic susceptibility and density values of the microparticles and cells are calculated simultaneously, based on specific analysis at the level of each cell and microparticle. Thus, the measurement sensitivity is also increased.
  • the other aim of the invention is to present a magnetic levitation-based system used for the measurement of the magnetic susceptibility value and density of microparticles and cells, where the measurement of the magnetic susceptibility and density of microparticles and cells is reduced to the size of a single microparticle and cell.
  • both density and magnetic susceptibility values are analyzed within a single chip. The calculation can be reduced to a single microparticle and cell size through the microfluidic chip.
  • Single cell analysis enables examination of biological, structural and chemical properties of the examined cell at single cell level.
  • analysis at single cell level constitutes importance in determining different cells in heterogeneous cell populations.
  • this analysis is divided as labeled and label-free methods, and existing label-free methods are expensive and require an expert user.
  • Magnetic levitation principle enables label-free cells to rise by directing under magnetic field and to be analyzed accordingly.
  • magnetic susceptibility value of the cell is neglected in existing studies. On the contrary, this value is quite critical in structural separation of cell in single cell level analyses. Therefore, in the invention, magnetic susceptibility value and density value of cell are calculated simultaneously, offering specific analysis opportunity on that cell basis. Thus, sensitivity of the measurement is also increased.
  • the invention aims to introduce a cost-reduced magnetic levitation-based system for measuring the magnetic susceptibility and density of microparticles/cells.
  • magnetic susceptibility and density are measured using expensive devices, whereas in the system of this invention, both density and magnetic susceptibility can be analyzed within a single chip.
  • PMMA polymethylmethacrylate
  • a system that is highly user-friendly and effortless to operate is introduced for the measurement of the magnetic susceptibility and density of microparticles/cells.
  • This user-friendly and effortless system for measuring the magnetic susceptibility and density of microparticles/cells is achieved in the invention by introducing the samples into the microchannel without requiring any prior preparation, measuring the heights in two regions of paramagnetic solutions with different concentrations, and inserting these values into the developed equation.
  • a new technique is presented that can perform analyses with a small sample volume, without requiring expensive equipment, and within a short time of approximately 10 minutes.
  • a magnetic levitation-based system for the measurement of magnetic susceptibility and density, offering the capability to examine the sample at two different positions along the microfluidic channel.
  • a magnet with a length of 25 mm is used, offering the capability to examine the sample at two different positions along the microfluidic channel.
  • the levitation height of the cell is examined at two different positions. In the first position, the cell, which reaches equilibrium in paramagnetic solution-l, moves under flow towards position-l I. A paramagnetic solution of different concentration is given from the top, and the paramagnetic solution-ll determined at position-ll is reached.
  • both the density and the magnetic susceptibility value can be calculated simultaneously with the obtained data.
  • using a magnet with a length of 25 mm is a very important parameter in determining the positions within the paramagnetic solution of two different concentrations on the microfluidic chip. Additionally, using a magnet with a length of 25 mm allows for analysis to be performed at a shorter distance.
  • the invention provides a magnetic levitation-based system that allows analysis to be performed over shorter distances, designed for measuring the magnetic susceptibility and density of microparticles. If the platform were produced with the long magnet (approximately 50 mm in length) used in standard magnetic levitation platforms, it would not be possible to achieve a small system design as proposed in the invention. Additionally, the use of a long magnet would alter the path taken by microparticles, thereby increasing the transition time between positions and extending the time required to track a single microparticle.
  • the invention provides a magnetic levitationbased system that allows analysis to be performed over shorter distances, made possible by a magnet with a length of 25 mm used for measuring the magnetic susceptibility and density of microparticles. Using a magnet with a length of 25 mm in the proposed invention is a important parameter for determining positions within two different concentrations of paramagnetic solution on a microfluidic chip. Furthermore, it enables analysis to be performed over shorter distances.
  • the microfluidic chip is positioned on top of the magnets.
  • the use of double-sided adhesive and PMMA in the production of the microfluidic chip provides a rapid and cost-effective manufacturing process compared to other production methods (such as soft lithography, photolithography, etc.).
  • positioning the microfluidic chip on top of the magnets without the need for it to be placed between the magnets simplifies the integration of the microfluidic chip, which can be produced in various designs, into the magnetic levitation system. So, in previous magnetic levitation systems, analysis could be performed when the microcapillary channel or channel was only between the magnets.
  • the microfluidic channel can be positioned on top of the magnets in the desired design. This also opens the way for the use of various microfluidic channels and their simple production unique to the magnetic levitation system. Thus, it has enabled the easy production of magnetic levitation-based measurement systems.
  • the microfluidic chip included in the magnetic levitation-based system proposed to the invention consists of three layers: the bottom layer being the glass slide, the middle layer being the double-sided adhesive cut with a laser cutter according to the channel shape, and the top layer being the polymethylmethacrylate (PMMA) plate with a thickness of 2 mm.
  • PMMA polymethylmethacrylate
  • the existing analyses of the magnetic levitation-based system proposed to the invention have been primarily conducted under a microscope.
  • the simultaneous calculation of magnetic susceptibility and density in the microfluidic chip is planned to be performed automatically on a portable microscopic platform.
  • a lensless holographic microscopy platform has been developed.
  • the noise that could be created by the polymethylmethacrylate (PMMA) plate has been minimized.
  • PMMA plate with a thickness of 2 mm has been considered suitable.
  • Figure 1 Simultaneous determination of the magnetic susceptibility and density properties of microparticles in a magnetic levitation system.
  • A Shows the behavior of microparticles under a magnetic field
  • B shows the behavior of microparticles in the microfluidic chip.
  • 1 microcapillaries
  • 2 top magnet
  • 3 bottom magnet
  • 4 magnetic induction
  • 5 cell/microparticle
  • 6 magnetic force
  • 7 buoyancy force of the liquid
  • 8 levitation height
  • 9 paramagnetic medium
  • 10 polymethylmethacrylate (PMMA) plate
  • 1 1 double-sided adhesive
  • 12 bottom glass slide
  • 13 microfluidic chip channel
  • 14 flow-1
  • 16 microfluidic chip.
  • FIG. 1 Microscopic images of the levitation heights of 1.05 g/mL polyethylene microparticles added to 50 and 75 mM Gadavist solutions. The scale bar is 200 pm.
  • B Calibration curve formed from (B . V)B values at different levitation heights in a magnetic levitation-based system containing microcapillaries.
  • 1 microcapillaries
  • 2 top magnet
  • 3 bottom magnet
  • 17 1.05 g/mL polyethylene microparticle.
  • FIG. 3 Micrographs of microparticles with different densities prepared in 50 and 75 mM Gadavist solutions, respectively.
  • 8 levitation height (pm)
  • 18 1.025 g/mL polyethylene (PE) microparticle
  • 19 1.07 g/mL polyethylene (PE) microparticle
  • 20 1.09 g/mL polyethylene (PE) microparticle
  • 21 polybutylmethacrylate (PBMA) microparticle
  • PMMA polymethylmethacrylate
  • FIG. 4 (A) The setup used for the simultaneous determination of the magnetic susceptibility and density properties of microparticles under flow in the microfluidic chip. (B) The equilibrium of microparticles under flow based on the properties of the paramagnetic solution at the first position and the second position. (C) Determination of the levitation height of microparticles at two different positions.
  • FIG. 1 Microscopic images of the levitation heights of 1 .09 g/mL PE microparticles in 30 and 50 mM Gd solutions within the microfluidic chip.
  • 2 top magnet
  • 3 bottom magnet
  • 29 30 mM Gadavist (Gd) solution
  • 30 50 mM Gadavist (Gd) solution.
  • the scale bar is 200 pm.
  • the production method of a magnetic levitation-based system, containing microcapillaries, used for the simultaneous measurement of magnetic susceptibility and density of microparticles and cells includes the following steps; i. producing the skeleton of the magnetic levitation platform to be used in the magnetic levitation-based system with a polylactic acid (PLA) filament via a three-dimensional (3D) printer, ii. positioning the same poles of the neodymium (N52) top magnet (2) and bottom magnet (3) to face each other, iii. positioning the microcapillaries (1 ) horizontally in the magnetic levitation platform, such that they are located between the top magnet (2) and bottom magnet (3).
  • PVA polylactic acid
  • the production method of a magnetic levitation-based system for the microfluidic chip (16) application used for the simultaneous measurement of magnetic susceptibility and density of microparticles and cells, includes the following steps; i. for the production of the microfluidic chip (16) ( Figure 1 B) to be used in the magnetic levitation-based system, first, the polimetylmethacrylate (PMMA) plate (10) and double-sided adhesive (1 1 ) are cut with a laser cutter, and then, the bottom glass slide (12), cleaned in an ultrasonic bath with ethanol, is attached to the polimetylmethacrylate (PMMA) plate (10) with the cut double-sided adhesive (11 ), ii. the microfluidic chip channel (13) is horizontally positioned between the neodymium (N52) top magnet (2) and bottom magnet (3).
  • the polimetylmethacrylate (PMMA) plate (10) and double-sided adhesive (1 1 ) are cut with a laser cutter, and then, the bottom glass slide (12), cleaned in an
  • the magnetic force (6) (Fm) and the buoyancy force of the liquid (7) (Fb), which are the balance forces at the levitation heights (8), are solved and the magnetic susceptibility and density of the microparticle are obtained simultaneously.
  • the image acquisition process in the process steps ii and iv is carried out using a microscope, lensless holography microscope or a smartphone camera.
  • the flow rate (Flow-1 (14)) mentioned is 0.01 -100 pL/min. In one application of the invention, the mentioned flow (Flow-1 (14)) has a rate of 0.1 pL/min.
  • Figure 1 is related to the simultaneous determination of the magnetic susceptibility and density properties of microparticles in the magnetic levitation system.
  • A a magnetic levitation-based system for the microcapillary application is shown; here, the microparticles reach equilibrium at the levitation height with the magnetic force (F m ) and the buoyancy force (F b ).
  • the magnetic susceptibility (/ p ) and density ( p ) properties of the microparticles are determined by solving the relevant force equations.
  • (A) contains the microscopic images of the levitation heights of 1 .05 g/mL polyethylene microparticles (17) added to 50 and 75 mM Gadavist solutions.
  • the dashed line shows the positions of the top magnet (2) and the bottom magnet (3).
  • the scale bar is 200 pm.
  • (B) shows the values of (B .7)B at different levitation heights in the system.
  • FIG 3 the micrographs of different density polyethylene microparticles that are 1.025 g/mL polyethylene microparticle (18), 1.07 g/mL polyethylene microparticle (19), and 1.09 g/mL polyethylene microparticle (20); polybutylmethacrylate (PBMA) microparticle (21 ) and polymethylmethacrylate (PMMA) microparticle (22) prepared in 50 and 75 mM Gadavist solution are shown, respectively.
  • the microparticles are expected to reach equilibrium after being introduced into the microcapillary. Images of the microparticles at equilibrium are captured. The images can be taken with a microscope, a lensless holographic microscope, or a smartphone camera. These images are then processed to calculate the magnetic levitation heights, and using these heights, the magnetic susceptibility and density are calculated from the resolved equilibrium forces.
  • the scale bar here is 250 pm.
  • FIG 4 shows the setup used for simultaneously determining the magnetic susceptibility and density properties of cells/particles (5) under flow in the microfluidic chip (16);
  • (B) shows the equilibrium of the particles under flow in position-l (23) and position-ll (24) depending on the properties of the paramagnetic solution.
  • position-l (23) a solution with different paramagnetic properties is mixed into the channel using Flow-2 (15), modifying the paramagnetic medium property, and paramagnetic solution-2 is obtained.
  • C the determination of the levitation heights of the microparticles at two different positions is shown.
  • the cell/microparticle (5) reaches equilibrium at different levitation heights (8) in position-l (23) and position-ll (24).
  • the relevant force equations are solved, and the magnetic susceptibility (/ P ) and density ( p ) properties of the cells are determined.
  • the magnetic levitation system consists of a neodymium top magnet (2) and bottom magnet (3) with their same poles facing each other, and a glass microcapillary (1 ) placed between the top magnet (2) and the bottom magnet (3).
  • the microparticles prepared in a paramagnetic medium (9) and placed into the microcapillary (1 ), reach a point where the magnetic force (Fm) and the buoyancy force of the liquid (Fb) balance each other, depending on the magnetic properties and density of the microparticles ( Figure 1 A). At the two equilibrium points, the forces are resolved (Equation 1 ), and the magnetic susceptibility and density values of the microparticles are calculated. Equation 1 .
  • Equation 1 / p and /m are the magnetic susceptibilities of the particles and the paramagnetic medium, po is the permeability of free space (1.2566 x 10" 6 kg m A" 2 s" 2 ), B is the magnetic induction (T) (4), p p ve p m are the densities of the particle and the medium; g is the gravitational acceleration (9.8 m s -2 ) and V is the volume of the particle. The point at which the particles reach equilibrium depends on the density and magnetic susceptibility of the particles.
  • the density of the reference microparticle ( p ), the magnetic susceptibility of the medium (/m), the density of the medium ( m ), po that is the permeability of free space and g that is the gravitational acceleration are known values, while % P , p P and (B . V)B are the unknowns.
  • a 1.05 g/mL polyethylene (PE) microparticle (Cospheric LLC., USA) with known density is selected as the reference particle to determine the values of (B . V)B at different levitation heights ( Figure 2A).
  • the levitation heights for this microparticle prepared in 5-350 mM Gd have been calculated.
  • the levitation heights (h) of polymethylmethacrylate (PMMA) in 50 mM and 75 mM Gd solutions have been measured.
  • the density of PMMA material is known to be 1.19 g/mL from the literature[9, 10].
  • the best magnetic susceptibility of the reference particle (XP.I.OS) was approximated from the levitation height-(B .
  • Equation 1 7)B values, using average h values for two different Gd concentrations, were used to calculate the density and magnetic susceptibility values of different microparticles using Equation 1. As shown in Table 1 , each microparticle group with the same density profile has different magnetic properties. To produce PE microparticles with different standard densities, different materials must be added to the PE material. This also modifies the magnetic susceptibility values[1 1 ]. In the calculations, the measured density values were very close to the values in the microparticle data sheets. There is only a 0.6% error in the calculations made according to the data sheets.
  • the magnetic levitation-based system proposed to the invention can also perform measurements on a single microparticle/cell basis. For this, the initial equilibrium height of the cells/microparticles moving under flow within the channel was determined, and then these cells/microparticles moving under flow were mixed with a liquid with different magnetic properties to determine the second equilibrium height.
  • the microfluidic chip (16) ( Figure 1 B) has been produced.
  • the microfluidic chip (16) to be used in the magnetic levitation system consists of a polymethylmethacrylate (PMMA) plate with 2 mm thickness (10), a bottom glass slide (12) (60 x 24 x 0.1 mm), and a 75 pm thick double-sided adhesive (1 1 ) (Thorlabs, USA).
  • the polymethylmethacrylate (PMMA) plate (10) and double-sided adhesive (1 1 ) have been cut with a laser cutter (Makeblock LaserBox, China).
  • the bottom glass slide (12), cleaned with a 70% ethanol into the ultrasonic bath, has been bonded to the polymethylmethacrylate (PMMA) plate (10) with the cut double-sided adhesive (1 1 ).
  • the microfluidic chip channel (13) has been positioned horizontally between the neodymium (N52) top magnet (2) and bottom magnet (3) (25 mm length, 2 mm width, and 5 mm height).
  • the microscopic image of the microparticles in the static state within the microchannel was taken every 5 minutes, and it was observed that the particles reached equilibrium within 10 minutes.
  • the image acquisition process can also be performed using a lensless holographic microscope or a smartphone camera.
  • the flow rate was calculated.
  • the heights at which the PE microparticles reached equilibrium in two different Gd solutions were observed ( Figure 4).
  • the magnetic induction values and the magnetic susceptibility and density values of the solution containing the microparticles/cells were used to solve the corresponding force equations, and the magnetic susceptibility and density of the cells/microparticles were calculated.
  • the density of the microparticle known to have a density of 1 .09 g/mL was calculated as 1 .081 g/mL, and its magnetic susceptibility value was calculated as -2.3308 x w 5 .
  • the calculated density value is close to the values in the microparticle's data sheets. This demonstrates that precise measurements can also be performed at the single microparticle/cell level in the microfluidic channel.

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  • Immunology (AREA)
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Abstract

La présente invention concerne un système à base de lévitation magnétique utilisé pour mesurer simultanément la susceptibilité magnétique et la densité de microparticules et de cellules. Le système à base de lévitation magnétique selon l'invention comprend deux aimants en néodyme (N52) ayant les mêmes pôles se faisant face, et un microcapillaire en verre ou une puce microfluidique constitué d'une plaque de polyméthacrylate de méthyle (PMMA), d'une lame de verre et d'un adhésif double face.
PCT/TR2025/050107 2024-03-12 2025-02-11 Système à base de lévitation magnétique pour la mesure simultanée de la susceptibilité magnétique et de la densité de microparticules et de cellules Pending WO2025193197A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2024002998 2024-03-12
TR2024/002998 TR2024002998A1 (tr) 2024-03-12 Mi̇kroparçaciklarin ve hücreleri̇n manyeti̇k alinganlik ve özkütleleri̇ni̇n eş zamanli olarak ölçülmesi̇nde kullanilmak üzere manyeti̇k levi̇tasyon temelli̇ bi̇r si̇stem

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WO2025193197A1 true WO2025193197A1 (fr) 2025-09-18

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021236044A1 (fr) * 2020-05-18 2021-11-25 İzmi̇r Yüksek Teknoloji̇ Ensti̇tüsü Plate-forme de tri de cellules microfluidiques reposant sur un principe de lévitation magnétique
US20220152628A1 (en) * 2019-03-15 2022-05-19 Christoffer Karl Abrahamsson Magnetic levitation system
JP2023081880A (ja) * 2015-10-02 2023-06-13 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 磁気浮上を用いた生物学的および非生物学的な部分の選別

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023081880A (ja) * 2015-10-02 2023-06-13 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 磁気浮上を用いた生物学的および非生物学的な部分の選別
US20220152628A1 (en) * 2019-03-15 2022-05-19 Christoffer Karl Abrahamsson Magnetic levitation system
WO2021236044A1 (fr) * 2020-05-18 2021-11-25 İzmi̇r Yüksek Teknoloji̇ Ensti̇tüsü Plate-forme de tri de cellules microfluidiques reposant sur un principe de lévitation magnétique

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