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WO2025190722A1 - Système modulaire pour cartouche microfluidique, en particulier pour analyser un échantillon biologique - Google Patents

Système modulaire pour cartouche microfluidique, en particulier pour analyser un échantillon biologique

Info

Publication number
WO2025190722A1
WO2025190722A1 PCT/EP2025/055785 EP2025055785W WO2025190722A1 WO 2025190722 A1 WO2025190722 A1 WO 2025190722A1 EP 2025055785 W EP2025055785 W EP 2025055785W WO 2025190722 A1 WO2025190722 A1 WO 2025190722A1
Authority
WO
WIPO (PCT)
Prior art keywords
module
modular system
fluid
modules
cartridge
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2025/055785
Other languages
German (de)
English (en)
Inventor
Martin Schulz
Juergen Steigert
Marc Meier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of WO2025190722A1 publication Critical patent/WO2025190722A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/028—Modular arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Definitions

  • Microfluidic systems can be used for medical diagnostic procedures to be performed at the point of care, particularly assays for the detection of pathogens using nucleic acid amplification.
  • These systems such as the Vivalytic® platform from Robert Bosch GmbH, often comprise a cartridge for receiving and processing the sample, and an analyzer for receiving the cartridge and controlling the processing of the sample in the cartridge.
  • the cartridge comprises a fluidic network and upstream reagents for processing the sample. It is typically designed as a disposable part so that it can be disposed of with the processed sample still contained within it after use.
  • the microfluidic cartridge is formed by combining the carrier cartridge with the physical interface and one or more modules.
  • the invention thus provides a modular system and, in particular, a modular microfluidic cartridge, which represents a modular platform for application-oriented combinations of modules and interfaces with the carrier cartridge for the realization of customized microfluidic cartridges.
  • This has the advantage that, by combining different, particularly standardized, modules with the carrier cartridge, various assays can be easily implemented in a resource-efficient manner.
  • the invention allows the separation of different functions on a microfluidic cartridge into two basic types: A first type of generic functions, which are identical for a variety of different assays or tests, and a second type of special functions, which are either specific to the respective assay or test and/or are consumed during the performance of the assay or test.
  • the first type includes, for example, certain reagents such as wash buffers or lysis reagents.
  • the first type includes components for which the modules only need to be refilled as needed, while the second type includes structures such as specific fluid networks or, in particular, functionalized microarrays, which must be replaced as modules in their entirety.
  • the modules can in particular be one or more fluid modules, sample input modules and/or reagent modules, as described below. These modules can each be part of the modular system.
  • the modules are preferably designed for a reversible connection to the carrier cartridge, for example as inserts or insert chips for a mechanical, magnetic or adhesive (in particular via reversible adhesive bonds) connection to the carrier cartridge.
  • one or more module areas can be designed to irreversibly accommodate modules, for example via a snap-in connection that cannot be removed without destruction. Irreversible accommodation is particularly useful if the irreversibly arranged module is not to be replaced by another module after use, for example for safety or hygiene reasons.
  • the physical interface can also be designed as a module, in which case the interface area is also designed as a module area.
  • modules for example, a fluid module or a reagent module, can also comprise a physical interface to the analytical device, in particular a pneumatic interface, for example, comprising one or more pneumatic connections, or a mechanical interface, for example, a movable part within the module, such as a plunger, which can be moved by an actuator of the analytical device.
  • a pneumatic interface for example, comprising one or more pneumatic connections
  • a mechanical interface for example, a movable part within the module, such as a plunger, which can be moved by an actuator of the analytical device.
  • the carrier cartridge preferably comprises a fluid module region for receiving a fluid module.
  • the fluid module comprises fluid channels and in particular a fluidic network for transporting a sample and/or reagents received in the cartridge.
  • the fluid module can be part of the modular System.
  • the fluidic network can have chambers connected via channels, one or more branching channels, filters, and/or valves.
  • a chamber of the network can comprise an array or microarray, in particular a cavity array, for a biochemical reaction, in particular for carrying out nucleic acid amplification.
  • the fluid module, in particular the network can have one or more upstream reagents, in particular beads, for biochemical reactions, in particular for nucleic acid amplification.
  • the modular system comprises at least a first fluid module and a second fluid module, wherein the two fluid modules are designed differently.
  • One or both fluid modules can have at least one chamber, at least one bead and/or an array, in particular a microarray, for carrying out nucleic acid amplification.
  • the first fluid module has at least two or at least three chambers fluidically connected in series for carrying out a PCR and preferably at least one bead for a PCR.
  • the second fluid module has at least one chamber with an array, in particular a cavity array for carrying out nucleic acid amplification in the cavities.
  • the modular system in particular a fluid module, comprises an array, in particular a microarray or a cavity array
  • reagents can be pre-stored, in particular freeze-dried, on the array, in particular on the surface or in the cavities, and/or entities, in particular molecules such as oligonucleotides, can be immobilized, in particular as capture molecules.
  • the carrier cartridge can comprise a module area designed for reagent pre-storage as a pre-storage area and preferably one or more reagent reservoirs attachable in the pre-storage area as reagent modules.
  • the reagent modules can be part of the modular system.
  • the carrier cartridge may have a sample input area as a module area for a sample input module.
  • the sample input area is located adjacent to the pre-storage area and/or the fluid module area in order to bring a sample introduced into the sample input module into contact with reagents over a short distance or to transport it via the fluid module.
  • the sample input area can also be located in the pre-storage area.
  • one or more of the module areas are preferably designed to reversibly accommodate modules. These modules can, in particular, be removed from the module areas again without leaving any residue and without damaging the carrier cartridge.
  • the reversible accommodation and fastening of the modules on the carrier cartridge can, in particular, be realized by a positive, magnetic, adhesive, and/or force-fitting connection, for example a snap-in or click-in connection or a reversible adhesive tape or glue connection.
  • the interchangeability of the modules advantageously allows reuse, in particular of the carrier cartridge, by simply replacing used or consumed modules with new and/or different modules, especially when used for conducting a different assay.
  • one or more module areas can be designed to irreversibly accommodate modules, for example, positively using barbs or firmly bonded using adhesive.
  • At least one of the module areas can be designed to accommodate different modules, in particular modules of different sizes and/or modules with different connecting elements that match the respective connecting elements of the module areas, in particular based on different complementary structures based on the key-lock principle and preferably on the Poka-Yoke principle. This expands the advantageous multifunctionality of the modular system.
  • the carrier cartridge can also have one or more multifunctional module areas and/or multi-module areas.
  • a multifunctional module area is designed to accommodate a first module or, alternatively, a second module.
  • a multi-module area is designed to reversibly accommodate one module or, alternatively, at least two other modules simultaneously. Both the multifunctional module areas and the multi-module areas advantageously contribute to space optimization on the carrier cartridge, in addition to a wide range of possible functions.
  • the physical interface can, in particular, comprise a pneumatic interface, or pneumatic interface for short.
  • the pneumatic interface can comprise valves and pneumatic channels connected to the valves to support microfluidic operations on the cartridge.
  • the interface area of the carrier cartridge preferably comprises pneumatic channels and connections for contacting pneumatic connections of the pneumatic interface.
  • the physical interface is preferably part of the modular system. According to a particular embodiment, the physical interface is firmly connected to the interface area and, in particular, is formed as part of the carrier cartridge.
  • the interface area for arranging the physical interface in particular the pneumatic interface, can be arranged between one or more pre-storage areas and other module areas, in particular the fluid module area on the carrier cartridge.
  • the interface can act as an intermediary between the reagents and other modules, in particular fluid modules.
  • the carrier cartridge can comprise at least two layers, in particular a pneumatic layer and a fluidic layer, and a membrane arranged between the two layers, wherein one of the two layers or both layers have the module regions.
  • Figure 1 shows an example of a microfluidic cartridge known from the prior art
  • FIGS 2a-g show embodiments of the modular system according to the invention and the modular microfluidic cartridge 2000 according to the invention.
  • microfluidic cartridges can be processed with an analysis device, as described, for example, in the documents DE 10 2016 222 075 A1 and DE 10 2016 222 072 A1.
  • the microfluidic cartridge can be intended in particular for the analysis of biological samples, for example for the analysis of body fluids such as sputum, blood, urine, or a smear.
  • the analysis can comprise the detection of pathogens or other biological substances via genetic detection, in particular via nucleic acid amplification.
  • Such a cartridge can preferably be used at the point of care.
  • Figure 1 shows a top view of an example of a previously known microfluidic cartridge 10, which can be used for the Vivalytic® platform from Robert Bosch GmbH.
  • the cartridge 10 has a sample input chamber 18, which is closed with a foldable lid 19 after the sample has been introduced.
  • Figure 1a already shows the closed state.
  • the cartridge 10 comprises a storage area 11 for reagents in the rear part, which can be stored in the cartridge 10, for example, in the form of nine reagent bars.
  • the front part of the cartridge 10 comprises a processing area 13, partially transparent from above, with a fluidic network 14 and a chamber 15 for a microarray.
  • the fluidic network 14 has, for example, two strands, each with three chambers connected in series.
  • the chambers can be heated to different temperatures, particularly for carrying out two- or three-step polymerase chain reactions (PCR).
  • PCR polymerase chain reactions
  • a pneumatic interface 12 with connections arranged in two rows in the form of pneumatic ports for connecting a pressure source of the analysis device in order to pneumatically control the fluidic operations in the cartridge.
  • the cartridge 10 has a layered structure, with a fluid layer comprising the majority of the cartridge's entire fluidic network and a pneumatic layer, as well as an expandable membrane layer arranged between the fluid layer and the pneumatic layer.
  • the membrane layer is expanded locally in chambers of the fluidic layer and pneumatic layer by the pressures applied via the pneumatic interface, depending on the process, in order to move fluids and reagents within the cartridge.
  • the fluidic network is permanently installed in the cartridge, and the reagents cannot be removed or replaced after insertion during production without damaging the cartridge.
  • Figure 2a shows an embodiment of the modular system 1000 according to the invention.
  • the system 1000 can be part of a microfluidic cartridge 2000, thus Figure 1b also shows an embodiment of the modular microfluidic cartridge 2000 according to the invention.
  • the system 1000 comprises a carrier cartridge 100, wherein the carrier cartridge 100 in this example has three module areas 110, 120, 130, namely a pre-storage area 110, a fluid module area 130 and an interface area 120 arranged between the pre-storage area 110 and the fluid module area 130.
  • the interface area 120 is designed to arrange a physical interface 121 to a processing device, also called a processing unit, in particular an analysis device, and can in particular have a pneumatic interface 121 with connections 122, in particular pneumatic ports 122 (in the example in Figure 2a comprise two rows of seven ports 122 each for pneumatic actuation of the system by the processing device.
  • the physical Interface 121 and in particular the pneumatic interface 121 with its connections 122 can be firmly connected to the carrier cartridge 100 or even form part of the carrier cartridge 100 and thus of the interface region 120.
  • Figure 2a thus shows a preferred embodiment of the modular system 1000 according to the invention comprising the carrier cartridge 100 with physical interface 121, in particular pneumatic interface 121, as a preferably fixed component of the carrier cartridge 100, wherein reagent modules can preferably be arranged reversibly in the pre-storage region 110 and/or fluid modules can preferably be arranged reversibly in the fluid module region 130.
  • the illustration in Figure 2a is also intended to indicate that the functions of the three module areas 110, 120, 130 and in particular of the interface area 120 are preferably located at the same location as in a previously known cartridge (as in Figure 1a) in order to enable backward compatibility of the modular cartridge with a processing device used for the previous cartridge, in particular for the usability of existing interfaces in the processing device (for example, pneumatic interface or interface for optical readout of the processing area of the cartridge).
  • the carrier cartridge can have channels 101, 102, in particular between the areas 110, 120, 130, in order to enable fluid transport and/or a pneumatic connection between the module areas 110, 120, 130 and in particular between modules accommodated in the areas 110, 120, 130.
  • the carrier cartridge 100 preferably has openings 103, 104 for fluidic connection to the modules, i.e., in particular, to the fluid modules, reagent modules, and the physical interface.
  • the openings 103, 104 can be designed to be closable, for example, with valves.
  • the carrier cartridge 100 preferably comprises channels (not shown) that at least partially interconnect the openings 103, 104, so that the modules arranged in the module areas 110, 120, 130 can communicate fluidically with one another.
  • the carrier cartridge 100 can, for example, like the known cartridge 10 shown in Figure 1, have a layered or stratified structure.
  • the carrier cartridge 100 can have two layers, a pneumatic layer and a fluidic layer, and a membrane arranged between the two layers, wherein at least one of the two layers has the module regions.
  • Recesses can be provided in one or both layers in the module regions to allow expansion of the membrane into openings in the modules.
  • one or more layers can have the openings 103, 104 and channels described above.
  • the layers and the membrane can be made of plastics, for example the layers of polycarbonate and the membrane of thermoplastic urethane (TPU).
  • the modules are preferably designed for a reversible connection to the associated module area, for example as inserts or insert chips for a mechanical, magnetic or adhesive (in particular via reversible adhesive bonds) connection to the carrier cartridge, as also described above for the reagent containers.
  • Figures 2b and 2c show two advantageous embodiments of the pre-storage area 110 as a modular area.
  • the pre-storage area comprises several, for example four, sub-areas 111, 112, 113, 114, in each of which a reagent reservoir can be arranged as a reagent module, in particular in the form of a reagent container.
  • the four sub-areas 111, 112, 113, 114 thus also form modular areas in themselves.
  • the reagent container can be a preferably refillable plastic container. Alternatively, it can also be a reagent bar, for example.
  • the storage area can have recesses into which the reagent reservoirs or reagent containers can be inserted.
  • elastic locking hooks or locking lugs 105 and/or movable lids can be arranged in the storage area or on the recesses, as indicated by way of example in Fig. 2b.
  • magnetic anchoring is possible, for example via permanent magnets in the storage area and magnetic or magnetizable material, for example in the bottom or side area of the reagent containers.
  • Figure 2c also shows the configuration of the pre-storage area 110 into several, for example, four, sub-areas 111, 112, 113, 114, wherein the sub-areas are configured as refillable reagent containers 111, 112, 113, 114, which are particularly permanently installed or even form a seamless part of the carrier cartridge.
  • the refillable reagent containers each have an opening 115, 116, 117, 118 that can be closed, for example, with a clip-on or screw-on lid.
  • Figure 2c also shows, by way of example, that the reagent containers 111, 112, 113, 114 can have different sizes.
  • the release of the reagents from the reagent reservoirs can be achieved by an actuation unit in the pre-storage area 110 or in the reagent container itself, for example, by active suction using the pneumatic interface.
  • the release can be achieved by actuation mechanisms of the processing device, for example, by plungers of the processing device, which displace the reagents from the reservoirs 111, 112, 113, 114 into channels or chambers of the cartridge.
  • the carrier cartridge 100 may further comprise a region for receiving a sample input module 150, wherein the sample input module 150 may comprise a sample input chamber with a closable lid 151.
  • This region may be formed as a standalone region or as a sub-region of another module region on the carrier cartridge, for example, as indicated in Figure 2c, as sub-region 119 of the pre-storage region 110.
  • Figures 2b-g show advantageous embodiments of the fluid module region 130.
  • the fluid module region can, in particular, be designed to accommodate one or more fluidic modules.
  • Figures 2b and 2c show examples with only one fluid module 138 in the fluid module region 130.
  • the fluid modules can have channels and/or chambers, in particular as part of fluidic networks, in particular for carrying out molecular biological tests or assays.
  • the fluid modules can have filters (in particular in the channels or chambers), valves, and upstream reagents such as beads for PCR.
  • at least one fluid module comprises chambers for carrying out nucleic acid amplification, for example in the form of PCR or isothermal amplification.
  • a fluid model can have several chambers connected in series (also referred to as strands), in particular for carrying out a shuttle PCR, wherein fluid containing nucleic acids to be amplified is repeatedly transported through the chambers between differently temperature-controlled chambers for the realization of the PCR cycle denaturation - primer hybridization - elongation.
  • a strand can have two or three chambers for carrying out a two-stage and/or three-stage PCR.
  • the fluid module can, for example, also comprise two strands 141, 142 for the (parallel) execution of two PCRs.
  • the fluid module can also have a chamber 143 with preferably a microarray 144, for example comprising silicon, for molecular biological reactions, for example for the immobilization of molecules, for example enzymes, or hybridization of nucleic acid sequences to immobilized entities (for example capture molecules).
  • Such an array 144 can also be designed as a cavity array with reagents, such as primers, preferably pre-stored therein, in order to allow reactions to take place in the cavities formed as recesses or wells on the surface of the array, in particular polymerase chain reactions or isothermal nucleic acid amplifications.
  • reagents such as primers
  • One or more of the chambers of the strands 141, 142 and/or the chamber 143 with the microarray 144 can have at least partially transparent covers in order to observe a reaction taking place in the chambers and, in particular, to use fluorescent radiation emerging from the chambers for a particularly quantitative tive detection of the reaction products, for example when performing quantitative real-time PCR or when fluorescence excitation of entities hybridized on the microarray 144.
  • the fluid module region 130 can in particular be designed to accommodate a plurality of fluid modules, for example, as shown in Figure 2d, four fluidic modules 131, 132, 133, 134, which can comprise different fluidic networks as shown.
  • modules can advantageously be arranged in the fluid module area 130, and the required reagents can preferably be kept in the pre-storage area 110.
  • the modules in particular the fluidic modules, can be chips for sample purification, classical (q)PCR amplification, multiplex PCR, and also optical detection.
  • chips for implementing immunoassays, clinical chemistry, hematology, or cell imaging can be inserted.
  • the number of modules to be used can vary.
  • the fluid module region 130 can have a multifunctional module region and/or a multi-module region or be configured as such.
  • a multifunctional module region is designed to accommodate a first module or alternatively at least a second module, while a multi-module region is designed to reversibly accommodate one module or alternatively at least two other modules simultaneously.
  • Figures 2d to 2g show an example in which the fluid module region 130 is designed both as a multifunctional module region and as a multi-module region (the other regions of the cartridge 100 are shown only in an abstract manner).
  • the fluid module region 130 can accommodate up to four fluid modules 131, 132, 133, 134, which can be different as required and which can be fixed, for example, with elastic locking hooks or locking lugs 105.
  • the fluid modules can be attached analogously to the reagent reservoirs described above.
  • the fluid module area 130 can alternatively, three, two or just one fluid module can be accommodated, wherein these fluid modules can have different sizes, in particular approximately integer multiples of the size relative to one another.
  • a fifth fluid module 135 can be arranged in the fluid module region instead of two fluid modules 131, 132 from Figure 2d, wherein the fifth fluid module can have a size up to the sum of the sizes of the two fluid modules 131, 132 from Figure 2d, in particular twice the size compared to the size of the fluid modules from Figure 2d.
  • Figure 2f shows the accommodation of the fifth fluid module 135 and a sixth fluid module 136, wherein the sixth fluid module 136 also takes the space of two fluid modules 133, 134 from Figure 2d.
  • Figure 2g shows a seventh fluid module 137, which occupies the space of up to three fluid modules 131, 132, 133 from Figure 2d.
  • the fluid module area 130 can also accommodate only a single fluid module 138 instead of the four fluid modules 131, 132, 133, 134 from Figure 2d, as already shown in Figures 2b and 2c.
  • the carrier cartridge 100 with a preferably permanently installed physical interface, in particular a pneumatic interface and at least one fluidic module as well as a preferably multifunctional fluid module area and/or fluid multi-module area represents a further preferred embodiment of the system 1000 according to the invention.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un système modulaire (1000) pour une cartouche microfluidique (2000), en particulier pour la détection d'agents pathogènes, comprenant une cartouche de support (100), la cartouche de support (100) ayant une région d'interface (120) pour agencer une interface physique (121) par rapport à un analyseur et une ou plusieurs régions de module (110, 130) pour agencer des modules (111‗112‗113, 114‗131‗132, 133‗134‗135, 136, 137, 138). L'invention concerne en outre une cartouche microfluidique modulaire (2000) comprenant un tel système modulaire (1000). La cartouche (2000) peut être destinée en particulier à l'analyse d'échantillons biologiques, par exemple des fluides corporels, et notamment à la détection d'agents pathogènes, de préférence au niveau du point de soins.
PCT/EP2025/055785 2024-03-13 2025-03-04 Système modulaire pour cartouche microfluidique, en particulier pour analyser un échantillon biologique Pending WO2025190722A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102024202356.6 2024-03-13
DE102024202356.6A DE102024202356A1 (de) 2024-03-13 2024-03-13 Modulares System für eine mikrofluidische Kartusche, insbesondere zur Analyse einer biologischen Probe

Publications (1)

Publication Number Publication Date
WO2025190722A1 true WO2025190722A1 (fr) 2025-09-18

Family

ID=94924868

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2025/055785 Pending WO2025190722A1 (fr) 2024-03-13 2025-03-04 Système modulaire pour cartouche microfluidique, en particulier pour analyser un échantillon biologique

Country Status (2)

Country Link
DE (1) DE102024202356A1 (fr)
WO (1) WO2025190722A1 (fr)

Citations (5)

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