[go: up one dir, main page]

WO2025173649A1 - Micro-organisme ayant la capacité à lutter contre les maladies des plantes - Google Patents

Micro-organisme ayant la capacité à lutter contre les maladies des plantes

Info

Publication number
WO2025173649A1
WO2025173649A1 PCT/JP2025/004054 JP2025004054W WO2025173649A1 WO 2025173649 A1 WO2025173649 A1 WO 2025173649A1 JP 2025004054 W JP2025004054 W JP 2025004054W WO 2025173649 A1 WO2025173649 A1 WO 2025173649A1
Authority
WO
WIPO (PCT)
Prior art keywords
culture
strain
plant
present
microorganism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/JP2025/004054
Other languages
English (en)
Japanese (ja)
Inventor
健太郎 西村
良典 水井
智和 荻野
千尋 西野
誉 山中
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nippon Soda Co Ltd
Original Assignee
Nippon Soda Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Soda Co Ltd filed Critical Nippon Soda Co Ltd
Publication of WO2025173649A1 publication Critical patent/WO2025173649A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P5/00—Nematocides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Definitions

  • the present invention relates to microorganisms capable of controlling plant diseases.
  • This application claims priority to Japanese Patent Application No. 2024-021425, filed February 15, 2024, the contents of which are incorporated herein by reference.
  • Bio pesticides are known to have advantages over conventional synthetic chemical pesticides, such as a lower environmental impact, fewer restrictions on their use to avoid crop residues, and a lower risk of developing resistant bacteria.
  • Biological pesticides that have been used to control plant diseases in agricultural and horticultural crops, particularly gray mold include microorganisms belonging to the genera Trichoderma, Gliocladium, Pseudomonas, and Bacillus, and agricultural and horticultural fungicide compositions containing these microorganisms have also been researched and developed.
  • Bacillus microorganisms that can be used to control plant diseases in agricultural and horticultural crops include Bacillus subtilis strain 4-5-1-1 No. 30 (Patent Document 1), Bacillus sp. strain 4-5-21 0306 (Patent Document 1), Bacillus sp. strain AT-332 (Patent Document 2), and Bacillus sp. strain AT-79 (Patent Document 2).
  • pesticides containing microorganisms of the genus Bacillus that can be used to control plant diseases include, for example, "Agrocare Wettable Powder” (Non-Patent Document 1), which contains viable spores of the Bacillus subtilis HAI-0404 strain as its active ingredient, "Serenade ASO,” which contains viable spores of the Bacillus subtilis QST-713 strain as its active ingredient, "Eco Shot,” which contains viable spores of the Bacillus subtilis D747 strain as its active ingredient, and "Impression Clear,” which contains viable spores of the Bacillus amyloliquefaciens AT-332 strain as its active ingredient.
  • “Agrocare Wettable Powder” Non-Patent Document 1
  • Serenade ASO which contains viable spores of the Bacillus subtilis QST-713 strain as its active ingredient
  • Esco Shot which contains viable spores of the Bacillus subtilis D747 strain as its active ingredient
  • Impression Clear which contains
  • All four of these pesticides contain live spores (i.e., bacterial cells) of Bacillus microorganisms as their active ingredient.
  • the culture supernatant obtained when culturing microorganisms generally contains metabolic products of the microorganisms, and these metabolic products may be rich in substances that have antagonistic effects against plant diseases.
  • culture supernatant can be obtained as a liquid component, different formulation designs and utilization methods may be possible compared to bacterial cells, which are solid components. Therefore, it is desirable to obtain microorganisms that can effectively utilize not only the bacterial cells contained in microbial cultures, but also the culture supernatant to control plant diseases.
  • the present invention was made in light of these circumstances. Specifically, the objective of the present invention is to provide a microorganism that has a low environmental impact, has excellent control properties against a variety of plant diseases, and allows not only the microbial cells but also the culture supernatant contained in the culture to be effectively used to control plant diseases.
  • the inventors collected, cultured, and isolated microorganisms from various plants, searching for microorganisms with strong activity. As a result, they discovered a microorganism that exhibits high control properties against a variety of plant diseases. Furthermore, it was shown that not only the microorganism itself but also the culture supernatant contained in the culture can be effectively used to control plant diseases. Furthermore, the nucleotide sequence of the microorganism's gene and its mycological properties strongly suggested that the microorganism may be a novel strain belonging to Bacillus amyloliquefaciens or Bacillus berezensis.
  • Bacillus sp. 201106_1 strain (NITE BP-03884 strain).
  • a mutant strain of Bacillus sp. 201106_1 (NITE BP-03884 strain), which has the ability to control plant diseases or nematodes.
  • [3] A culture of the strain described in [1] or [2] above.
  • [4] A processed product of the culture described in [3] above.
  • [5] The processed product according to [4] above, which is a culture supernatant.
  • a composition comprising the strain described in [1] or [2] above, a culture of the strain, or a processed product of the culture.
  • the mutant strain of the present invention may have, for example, the following nucleotide sequence i), ii), and/or iii). That is, it may have one or more of the nucleotide sequences selected from the group consisting of the following i), ii), and iii). It is also preferable that the mutant strain has all of the following nucleotide sequences i), ii), and iii).
  • a mutant strain of the invention may have, for example, the following nucleotide sequence: i) a nucleotide sequence having 99% or more identity to the nucleotide sequence of the 16S rRNA gene shown in SEQ ID NO: 3; ii) a nucleotide sequence having 99% or more identity to the nucleotide sequence of the phoR gene shown in SEQ ID NO: 6; and iii) a nucleotide sequence having 98% or more identity to the nucleotide sequence of the dam gene shown in SEQ ID NO: 9.
  • a mutant strain of the invention may also have, for example, the following nucleotide sequence: i) a nucleotide sequence having 99.8% or more identity to the nucleotide sequence of the 16S rRNA gene shown in SEQ ID NO: 3; ii) a nucleotide sequence having 99.8% or more identity to the nucleotide sequence of the phoR gene shown in SEQ ID NO: 6; and iii) a nucleotide sequence having 99% or more identity to the nucleotide sequence of the dam gene shown in SEQ ID NO: 9.
  • a mutant strain of the invention may also have, for example, the following nucleotide sequence: i) a nucleotide sequence having 100% identity with the nucleotide sequence of the 16S rRNA gene shown in SEQ ID NO: 3; ii) a nucleotide sequence having 100% identity with the nucleotide sequence of the phoR gene shown in SEQ ID NO: 6; and iii) a nucleotide sequence having 98% or more identity with the nucleotide sequence of the dam gene shown in SEQ ID NO: 9.
  • a mutant strain of the invention may also have, for example, the following nucleotide sequence: i) a nucleotide sequence having 100% identity with the nucleotide sequence of the 16S rRNA gene shown in SEQ ID NO: 3; ii) a nucleotide sequence having 100% identity with the nucleotide sequence of the phoR gene shown in SEQ ID NO: 6; and iii) a nucleotide sequence having 99% or more identity with the nucleotide sequence of the dam gene shown in SEQ ID NO: 9.
  • a mutant strain of the invention may also have, for example, the following nucleotide sequence: i) a nucleotide sequence having 100% identity with the nucleotide sequence of the 16S rRNA gene shown in SEQ ID NO:3; ii) a nucleotide sequence having 100% identity with the nucleotide sequence of the phoR gene shown in SEQ ID NO:6; and iii) a nucleotide sequence having 100% identity with the nucleotide sequence of the dam gene shown in SEQ ID NO:9.
  • having the ability to control plant diseases or nematodes means having an antagonistic effect against pathogenic bacteria or nematodes of any plant disease.
  • the microorganism of the present invention exerts an antagonistic effect against pathogenic bacteria or nematodes of plant diseases, thereby preventing or curing plant diseases or damage caused by the pathogenic bacteria or nematodes, and is particularly effective in preventing plant diseases.
  • preventing plant diseases caused by pathogens means that when plants or their cultivation soil that are not infected with pathogens of plant diseases or that do not show symptoms are grown under the same favorable conditions except for treating them with the microorganism of the present invention, the degree of damage caused by the disease in plants treated with the microorganism of the present invention is lower than that in plants that are not treated with the microorganism of the present invention.
  • curing a plant disease caused by a pathogen means that when a plant that has been infected with a pathogen of a plant disease and is showing symptoms is cultivated under the same favorable conditions except for treating it with the microorganism of the present invention, the severity of the disease in the plant treated with the microorganism of the present invention is lower than in a plant that has not been treated with the microorganism of the present invention.
  • preventing damage to plants caused by nematodes means that when plants or their cultivation soil that are not infected with or showing no damage from nematodes are grown under the same favorable conditions except for treating them with the microorganism of the present invention, the degree of damage to plants treated with the microorganism of the present invention is lower than that to plants that are not treated with the microorganism of the present invention.
  • curing plant damage caused by nematodes means that when plants infected with nematodes and showing damage are grown under the same favorable conditions except for being treated with the microorganism of the present invention, the degree of damage in plants treated with the microorganism of the present invention is lower than in plants not treated with the microorganism of the present invention.
  • the term “damage” is not particularly limited, but may be, for example, one or more selected from the group consisting of nematode invasion and parasitism; feeding damage by nematodes, tissue destruction, root gall formation, and absorption of water or nutrients; and plant wilting, growth inhibition, and poor appearance.
  • low degree of disease damage or “low degree of damage” may mean, for example, that the disease index, disease severity, or disease incidence rate is low, or that the control titer is greater than 0.
  • a higher control titer is preferable, with a value of 30 or higher being excellent, 50 or higher being even better, and 60 or higher, 70 or higher, 80 or higher, 90 or higher, or 100 being particularly excellent.
  • nematodes exhibit abnormal behavior (for example, symptoms of paralysis) as a result of treatment with the microorganism of the present invention, the paralysis will prevent the nematodes from invading or feeding on plants, and therefore the degree of damage is thought to be reduced.
  • Bacillus sp. 201106_1 strain was deposited on April 18, 2023 at the National Institute of Technology and Evaluation, Patent Microorganisms Depositary (NPMD) (Room 122, 2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture) under the international deposit number NITE BP-03884.
  • NPMD Patent Microorganisms Depositary
  • the mycological properties of Bacillus sp. 201106_1 strain are as follows: it is a gram-positive rod-shaped bacterium that produces acid aerobically and is capable of forming bacillus-type spores. Based on colony morphology, Bacillus sp. 201106_1 strain is presumed to be a bacterium of the genus Bacillus.
  • the method for culturing the microorganisms of the present invention is not particularly limited, and may involve growing the microorganisms by known means.
  • the substrate (culture medium, etc.) used during culturing may be liquid or solid.
  • the method for culturing the microorganisms of the present invention may involve culturing the microorganisms at 20 to 50°C using a substrate containing one or more selected from the group consisting of processed rice products, processed wheat products, processed corn products, processed potato products, processed soybean products, processed yeast products, processed meat products, processed seafood products, sugars, oils and fats, organic acids and their salts, inorganic acids and their salts, amino acids and their salts, and nucleic acids and their salts.
  • a culture of the microorganisms of the present invention can be obtained by such a method.
  • a culture of the microorganisms of the present invention can be obtained, for example, by any of the methods described below in Examples 2 to 4, or methods similar thereto.
  • culture and processed products thereof refers to a product obtained by culturing the microorganism of the present invention, and includes the cells of the microorganism of the present invention and/or its metabolic products (products).
  • the "culture” of the present invention may be used as is after culturing the microorganism of the present invention, or may be used as a processed product after undergoing some processing as necessary.
  • the "processed culture” of the present invention is not particularly limited in terms of the method or type of processing, and may be, for example, a processed product obtained by subjecting the culture to one or more processing steps selected from the group consisting of separation, filtration, disruption, extraction, purification, dilution, suspension, concentration, drying, freeze-drying, and spray-drying (i.e., one or more processing steps selected from the group consisting of an isolated product, a filtered product, an extract, a purified product, a diluted product, a suspended product, a concentrated product, a dried product, a freeze-dried product, and a spray-dried product of the culture).
  • the processed culture product is preferably a precipitate or a culture supernatant of the culture.
  • the precipitate of the culture mainly contains the cells of the microorganism of the present invention, and the cells may contain their metabolic products.
  • the culture supernatant may contain metabolic products released by the microorganism of the present invention.
  • the metabolic products may also include antibacterial or anti-nematode substances.
  • the culture supernatant does not need to be completely free of bacterial cells or their debris, and may contain solid components.
  • a culture supernatant can be defined as a liquid phase containing a metabolic product of the microorganism of the present invention.
  • the culture precipitate does not need to be completely free of liquid components, and may be mainly a solid phase containing bacterial cells of the microorganism of the present invention.
  • the culture precipitate or culture supernatant, or the bacterial cells or metabolic products contained therein may be further processed (e.g., one or more processes selected from the group consisting of separation, filtration, disruption, extraction, purification, dilution, suspension, concentration, drying, freeze-drying, and spray-drying) and used as a processed product.
  • the bacterial cell disruptant, cell fraction, or metabolic product obtained by such a process may be used as a specific component (e.g., an antibacterial or anti-nematode substance).
  • an extraction process may be performed.
  • extraction may be performed using a known solvent and a known method.
  • the solvent is not particularly limited, and may be, for example, an inorganic solvent or an organic solvent, more specifically, one or more selected from the group consisting of water, methanol, isopropanol, ethyl acetate, hexane, and mixtures thereof.
  • the culture of the present invention and a processed product thereof have the ability to control plant diseases or nematodes, and therefore can be effectively used for controlling plant diseases or nematodes.
  • Plant diseases The plant diseases (pathogenic fungi) to which the microorganism of the present invention is applied are not particularly limited, as long as the microorganism of the present invention can exert its control ability.
  • the microorganism of the present invention may be used, for example, to control bacterial diseases or fungal diseases, or may be used for both.
  • the microorganism of the present invention may also be used, for example, to control one or more plant diseases selected from the group consisting of plant diseases caused by bacteria belonging to obligate aerobic bacteria, microaerobic bacteria, and facultative anaerobic bacteria.
  • the microorganism of the present invention may also be used to control one or more plant diseases selected from the group consisting of plant diseases caused by filamentous fungi belonging to the phylum Oomycetes, Ascomycetes, Deuteromycetes, Basidiomycetes, and Zygomycetes.
  • the bacterial disease may be, for example, one or more selected from the group consisting of soft rot, rot, bacterial spot, bacterial leaf spot, canker, bacterial perforation, bacterial black spot, bacterial brown spot, bacterial stem necrosis, bacterial grain rot, bacterial seedling blight, bacterial wilt, bacterial leaf blight, black rot, and fire blight.
  • the fungal disease may be, for example, one or more filamentous fungal diseases selected from the group consisting of gray mold, brown rot, sclerotinia rot, brown spot, black spot, leaf spot, black spot, summer blight, ring spot, downy mildew, leaf mold, soybean scab, anthracnose, powdery mildew, rust, black spot, late blight, downy mildew, ring spot, blue mold, and green mold.
  • Peanuts Mycosphaerella arachidis, Ascochyta sp., Puccinia arachidis, Pythium debaryanum, Alternaria alternata, Sclerotium rolfsii, Mycosphaerella berkeleyi), black root rot (Calonectria ilicicola), etc.
  • Tomato Gray mold (Botrytis cinerea), leaf mold (Cladosporium fulvum), Phytophthora infestans, Verticillium albo-atrum, Verticillium dahliae, powdery mildew (Oidium neolycopersici), Alternaria solani, Pseudocercospora fuligena, Ralstonia solanacearum), Sclerotinia sclerotiorum, etc.
  • Eggplant Botrytis cinerea, Corynespora melongenae, Erysiphe cichoracearum, Mycovellosiella nattrassii, Sclerotinia sclerotiorum), Verticillium dahliae, Phomopsis vexans, etc.
  • chili pepper Phytophthora Phytophthora capsici
  • Botrytis cinerea Sclerotinia sclerotiorum, anthracnose (Colletotrichum aenigma, Colletotrichum capsici, Colletotrichum fructicola, Colletotrichum jiangxiense), powdery mildew (Leveillula taurica), etc.
  • rot disease Pseudomonas cichorii, Pseudomonas marginalis
  • soft rot Pectobacterium carotovorum
  • downy mildew Bremia lactucae
  • gray mold Botrytis cinerea
  • sclerotinia sclerotiorum big-vein disease
  • Mirafiori lettuce big-vein ophiovirus root rot
  • root rot Feusarium oxysporum
  • bottom rot Raizoctonia solani
  • powdery mildew Golovinomyces orontii
  • Green beans Sclerotinia sclerotiorum, gray mold (Botrytis cinerea), anthracnose (Colletotrichum lindemuthianum), angular spot (Phaeoisariopsis griseola), etc.
  • Peas Brown spot (Mycosphaerella pinodes), gray mold (Botrytis cinerea), sclerotinia sclerotiorum), powdery mildew (Erysiphe pisi), etc.
  • Plum black spot (Cladosporium carpophilum), gray mold (Botrytis cinerea), brown rot (Monilinia mumecola), sooty spot (Peltaster sp.), fruit blister (Taphrina pruni), brown hole (Phloeosporella padi), etc.
  • Persimmon powdery mildew (Phyllactinia kakicola), anthracnose (Gloeosporium kaki), angular leaf spot (Cercospora kaki), circular leaf spot (Mycosphaerella nawae), gray mold (Botrytis cinerea), sooty spot (Zygophiala jamaicensis), etc.
  • Grapes Gray mold (Botrytis cinerea), powdery mildew (Uncinula necator), late rot (Glomerella cingulata, Colletotrichum acutatum), downy mildew (Plasmopara viticola), black rot (Elsinoe ampelina), brown spot (Pseudocercospora vitis), black rot (Guignardia bidwellii), white rot (Coniella castaneicola), rust (Phakopsora ampelopsidis), white cotton snow (causative agent unidentified), crown gall (Rhizobium radiobacter, Rhizobium vitis), etc.
  • Pears Black spot (Venturia nashicola), red spot (Gymnosporangium asiaticum), black spot (Alternaria kikuchiana), ring spot (Botryosphaeria berengeriana), powdery mildew (Phyllactinia mali), canker (Phomopsis fukushii), brown spot (Stempphylium vesicarium), anthracnose (Glomerella cingulata), etc. Tea: ring spot (Pestalotiopsis longiseta, P.
  • Citri powdery mildew (Oidium sp.), late blight (Phytophthora citrophthora), anthracnose (Colletotrichum fioriniae), etc.
  • Kiwifruit blossom rot bacterial disease (Pseudomonas marginalis, Pseudomonas syringae, Pseudomonas viridiflava), canker (Pseudomonas syringae), gray mold (Botrytis cinerea), fruit soft rot (Botryosphaeria dothidea, Diaporthe sp., Lasiodiplodia theobromae), sooty spot (Pseudocercospora actinidiae), etc.
  • Olive anthracnose (Colletotrichum acutatum, Colletotrichum gloeosporioides), peacock spot (Spilocaea oleaginea), etc.
  • Chestnut anthracnose (Colletotrichum gloeosporioides) etc.
  • Powdery mildew (Blumeria graminis f.sp. tritici), Fusarium head blight (Gibberella zeae, Fusarium avenaceum, Fusarium culmorum, Fusarium crookwellense, Microdochium nivale), Fusarium rust (Puccinia recondita), Yellow rust (Puccinia striiformis), Brown snow rot (Pythium iwayamai), Monographella nivalis, Pseudocercosporella herpotrichoides, Septoria tritici, Leptosphaeria nodorum, Typhula incarnata, Myriosclerotinia borealis, Gaeumannomyces graminis), ergot disease (Claviceps purpurea), smut (Tilletia Barley: Spotted leaf disease (Pyrenophora), Barley: Barley: Spotted leaf disease (P
  • Tobacco sclerotinia sclerotiorum, powdery mildew (Erysiphe cichoracearum), late blight (Phytophthora nicotianae), etc.
  • Tulips Gray mold (Botrytis cinerea), brown spot (Botrytis tulipae), leaf rot (Rhizoctonia solani), bulb rot (Fusarium oxysporum), skin rot (Rhizoctonia solani), etc.
  • Roses Black spot (Diplocarpon rosae), powdery mildew (Erysiphe simulans, Podosphaera pannosa), gray mold (Botrytis cinerea), etc.
  • Chrysanthemums Gray mold (Botrytis cinerea), white rust (Puccinia horiana), downy mildew (Paraperonospora minor, Peronospora danica), Pythium damping-off (Pythium aphanidermatum, Pythium dissotocum, Pythium helicoides, Pythium oedochilum, Pythium sylvaticum), damping-off (Rhizoctonia solani), Fusarium wilt (Fusarium solani), etc.
  • Gerbera Gray mold (Botrytis cinerea), powdery mildew (Podosphaera xanthii), etc.
  • Lily Leaf blight (Botrytis elliptica, Pestalotiopsis sp.), Gray mold (Botrytis cinerea), etc.
  • Sunflower Downy mildew (Plasmopara halstedii), Sclerotinia sclerotiorum, Gray mold (Botrytis cinerea), etc.
  • Bentgrass Snow mold (Sclerotinia borealis), Large patch (Rhizoctonia solani), Brown patch (Rhizoctonia solani), Dollar spot (Sclerotinia homoeocarpa), Blast (Pyricularia sp.), Red burn (Pythium aphanidermatum), Anthracnose (Colletotrichum graminicola), etc.
  • Orchardgrass Powdery mildew (Erysiphe graminis), etc.
  • Soybean Purple spot (Cercospora kikuchii), downy mildew (Peronospora manshurica), stem rot (Phytophthora sojae), rust (Phakopsora pachyrhizi), sclerotinia sclerotiorum, anthracnose (Colletotrichum truncatum), gray mold (Botrytis cinerea), black rot (Elsinoe glycines), black spot (Diaporthe phaseolorum var.
  • Dioscorea leaf blight (Cylindrosporium dioscoreae), anthracnose (Colletotrichum gloeosporioides), blue mold (Penicillium sclerotigenum), etc.
  • Sweet potato purple root rot (Helicobasidium mompa), fusarium split (Fusarium oxysporum), root rot (Diaporthe destruens), etc.
  • Mango Anthracnose (Colletotrichum aenigma), canker (Xanthomonas campestris), stem rot (Diaporthe pseudophoenicicola, Lasiodiplodia theobromae, Lasiodiplodia spp., Neofusicoccum parvum, Neofusicoccum sp.), gray mold (Botrytis cinerea), etc.
  • Rapeseed Sclerotinia sclerotiorum), root rot (Phoma lingam), black spot (Alternaria brassicae), powdery mildew (Erysiphe cruciferarum, Erysiphe cichoracearum, Oidium matthiolae), downy mildew (Peronospora parasitica), etc.
  • Coffee rust (Hemileia vastatrix), anthracnose (Colletotrichum coffeanum), brown eye (Cercospora coffeicola), etc.
  • Sugarcane brown rust (Puccinia melanocephala), etc.
  • Cotton Seedling damping-off (Pythium sp.), rust (Phakopsora gossypii), white mold (Mycosphaerella areola), anthracnose (Glomerella gossypii), etc.
  • Hops Downy mildew (Pseudoperonospora humuli), powdery mildew (Oidium sp., Podosphaera macularis), gray mold (Botrytis cinerea), etc.
  • the nematodes to which the microorganism of the present invention is applied are not particularly limited, as long as the microorganism of the present invention can exert its control ability.
  • the microorganism of the present invention may be used, for example, to control nematodes that infest the above-ground parts (stems, leaves, buds, etc.) or underground parts (roots, tubers, etc.) of plants, preferably to control nematodes that infest the underground parts.
  • the nematodes that infest the underground parts may be, for example, one or more selected from the group consisting of root-knot nematodes (Meloidogyne spp.), root-lesion nematodes (Pratylenchus spp.), and cyst nematodes (Globodera spp. and Heterodera spp.), preferably root-knot nematodes.
  • the nematode may be one or more selected from these groups.
  • Tylenchida (a) Anguinidae, for example, Anguina funesta, Anguina tritici, from the genus Anguina; Ditylenchus destructor, Ditylenchus dipsaci, Ditylenchus myceliophagus, from the genus Ditylenchus; (b) Aphelenchoididae, for example, Aphelenchoides spp., such as rice root nematode (Aphelenchoides besseyi), strawberry nematode (Aphelenchoides fragariae), and peel nematode (Aphelenchoides ritzemabosi); Bursaphelenchus spp., such as pine wood nematode (Bursaphelenchus xylophilus); (c) from
  • Pratylenchus brachyurus such as Pratylenchus brachyurus, Pratylenchus coffeae, Pratylenchus curvitatus, Pratylenchus fallax, Pratylenchus goodeyi, Pratylenchus neglectus, Pratylenchus penetrans, Pratylenchus scribneri, Pratylenchus vulnus, Pratylenchus zeae); others include the false root-knot nematode (Nacobbus aberrans), the banana root-miner nematode (Radopholus similis), the citrus root nematode (Tylenchulus semipenetrans), and the citrus root-miner nematode (Radopholus citrophilus).
  • Pratylenchus brachyurus such as Pratylenchus coffeae, Pratylenchus curvitatus, Pratylenchus
  • Dorylaimida (a) From the family Longidoridae, for example, from the genus Longidorus, Longidorus elongates; from the genus Xiphinema, Xiphinema americanum, Xiphinema brevicolle, Xiphinema index, Xiphinema diversicaudatum.
  • Triplonchida (a) Family Trichodoridae, e.g., Trichodorus primitivus, Paratrichodorus minor.
  • the plants to which the microorganism of the present invention is applied are not particularly limited, as long as they are plants to which the microorganism of the present invention can exert its control activity.
  • the microorganism of the present invention may be used on one or more plants selected from the group consisting of, for example, grains; vegetables; root vegetables; potatoes; fruit trees, trees such as tea, coffee, and cocoa; pasture grasses; turf grasses; and cotton.
  • the plant may be, for example, one or more selected from the group consisting of plants belonging to the Brassicaceae family, Solanaceae family, Cucurbitaceae, Liliaceae, Fabaceae, Asteraceae, Chenopodiaceae, Poaceae, Rosaceae, Caryophyllaceae, Primulaceae, Rutaceae, Vitaceae, Actinidiaceae, Ebaceae, Apiaceae, Convolvulaceae, and Araceae families.
  • the plant in the present invention is preferably one or more selected from the group consisting of plants belonging to the Brassicaceae family, such as Chinese cabbage, plants belonging to the Asteraceae family, such as lettuce, plants belonging to the Solanaceae family, such as potato, plants belonging to the Rutaceae family, such as lemon and navel orange, and plants belonging to the Rosaceae family, such as pear.
  • Brassicaceae family such as Chinese cabbage
  • Asteraceae family such as lettuce
  • plants belonging to the Solanaceae family such as potato
  • plants belonging to the Rutaceae family such as lemon and navel orange
  • Rosaceae family such as pear.
  • the microorganisms of the present invention are not particularly limited in terms of the application site, and may be applied to one or more parts of plants, such as leaves, stems, stalks, flowers, buds, fruits, seeds, sprouts, roots, tubers, tuberous roots, shoots, and cuttings.
  • the microorganisms of the present invention may also be applied to improved varieties and cultivars, cultivated varieties, and even mutants, hybrids, or genetically modified organisms (GMOs) of the aforementioned plants.
  • the microorganisms of the present invention may be used in various treatments or applications for controlling various diseases that occur in agricultural and horticultural crops, including ornamental plants, turf, and pasture grass. For example, they may be used in one or more of the following: seed treatment, seed tuber treatment, foliar application, soil application, water application, and harvest treatment.
  • the microorganisms of the present invention may also be used as seed treatment agents, and any form of seed treatment known to those skilled in the art may be used, such as soaking, submerging, or coating seeds with a composition containing the microorganism of the present invention. Seed treatment is carried out before sowing seeds.
  • Harvest treatment may be treatment of plants before and/or after harvest, but is preferably treatment of plants after harvest (post-harvest treatment).
  • Non-agricultural uses As described above, the microorganism of the present invention can be used mainly for agricultural purposes, but may also be used for non-agricultural purposes.
  • Non-agricultural uses are not particularly limited, and may include, for example, use as one or more selected from the group consisting of a mildewproofing agent for walls of bathrooms, living rooms, etc.; a water quality improving agent for reservoirs, swimming pools, cooling towers, etc.; an agent for treating organic waste; and an agent for treating sludge, etc.
  • An effective amount means an amount sufficient to achieve the desired effect.
  • "as an active ingredient” also means that other ingredients may be included as long as they do not impair the effects of the present invention.
  • the effective amount may vary depending on the target of application, the purpose of application, the timing of application, etc.
  • the composition of the present invention contains the microorganism of the present invention, a culture thereof, or a processed product of the culture, but the form thereof is not particularly limited.
  • the composition of the present invention may contain the bacterial cells of the microorganism of the present invention itself, or a culture thereof itself, or may contain a processed product obtained by subjecting these to some treatment as necessary, as described above.
  • the processed product is not particularly limited, but is preferably a culture supernatant.
  • the microorganism of the present invention, a culture thereof, or a processed product of the culture thereof may be contained in the composition of the present invention in any form, such as a solid (solid phase), a liquid (liquid phase), or a mixture thereof.
  • concentration of the microorganism of the present invention, its culture, or a processed product of the culture contained in the composition of the present invention is not particularly limited as long as it does not impair the effects of the present invention, and may be, for example, in the range of 1 x 10 to 1 x 10 cfu/mL, preferably 1 x 10 to 1 x 10 cfu/mL, calculated as the bacterial cell concentration at the time of use of the composition.
  • the composition may be used by diluting the bulk or undiluted solution so that the bacterial cell concentration is within the above range at the time of use.
  • concentration of the microorganism of the present invention, its culture, or a processed product of the culture contained in the bulk or undiluted solution may be set appropriately depending on the dilution ratio at the time of use.
  • composition of the present invention may contain optional components other than the microorganism of the present invention, its culture, or a processed product of the culture, as long as the effects of the present invention are not impaired.
  • the optional components are not particularly limited as long as the effects of the present invention are not impaired, and may be, for example, one or more selected from the group consisting of carriers, diluents, surfactants, dispersants, and adjuvants.
  • one or more selected from the group consisting of antioxidants, colorants, lubricants, UV absorbers, antistatic agents, and preservatives may also be added.
  • composition of the present invention may be mixed with a chemical pesticide to the extent that it does not affect the microorganism of the present invention, its culture, or a processed product of the culture, and the chemical pesticide may be, for example, one or more selected from the group consisting of fungicides, insecticides, herbicides, and plant growth regulators.
  • a chemical pesticide such as a fungicide, insecticide, herbicide, or plant growth regulator has an effect on the microorganism of the present invention, it may be used by leaving an interval of several days between sprayings.
  • the carrier is not particularly limited as long as it can be used in ordinary agricultural and horticultural formulations, and may be one or more selected from the group consisting of inorganic salts such as calcium carbonate, potassium chloride, sodium sulfate, calcium sulfate, and ammonium sulfate; organic acids and salts thereof such as citric acid, malic acid, and stearic acid; sugars such as glucose, lactose, sucrose, maltose, and trehalose; and solid carriers such as alumina powder, silica gel, zeolite, hydroxyapatite, zirconium phosphate, titanium phosphate, titanium oxide, zinc oxide, hydrotalcite, kaolinite, montmorillonite, talc, clay, diatomaceous earth, bentonite, white carbon, kaolin, and vermiculite.
  • inorganic salts such as calcium carbonate, potassium chloride, sodium sulfate, calcium sulfate, and ammonium sulf
  • the diluent is not particularly limited as long as it is one that can be used in ordinary agricultural and horticultural formulations, and may be, for example, one or more selected from the group consisting of water, alcohol, acetone, ketone, pyrrolidone, sulfoxide, amide, glycol, nitrile, aromatic hydrocarbon, mineral oil, and vegetable oil.
  • the content of the diluent is not particularly limited, but for example, the blending ratio of the diluent per 1 part by mass of the microorganism of the present invention, its culture, or a processed product of the culture may be 0.1 to 100,000 parts by mass, preferably 1 to 50,000 parts by mass, more preferably 5 to 10,000 parts by mass, and even more preferably 10 to 5,000 parts by mass.
  • the surfactant (which can also be used as a dispersant) is not particularly limited as long as it is one that can be used in ordinary agricultural and horticultural formulations, and specifically, for example, it may be one or more selected from the group consisting of the following nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.
  • anionic surfactants include alkyl sulfate (C12-18, Na, NH 4 , alkanolamine), POE alkyl ether sulfate (C12-18, Na, NH 4 , alkanolamine), POE alkyl phenyl ether sulfate (C12-18, NH 4 , alkanolamine), POE benzyl (or styryl) phenyl (or phenylphenyl) ether sulfate (Na, NH 4 , alkanolamine), polyoxyethylene, polyoxypropylene block polymer sulfate (Na, NH 4 , alkanolamine); paraffin (alkane) sulfonate (C12-22, Na, Ca, alkanolamine), AOS (C14-16, Na, alkanolamine), dialkyl sulfosuccinate (C8-12, Na, Ca, Mg), alkyl benzene sulf
  • the amphoteric surfactant may be one or more selected from the group consisting of betaine surfactants such as dialkyl (C8-12) diaminoethyl betaine and alkyl (C12-18) dimethyl benzyl betaine; and glycine surfactants such as dialkyl (C8-12) diaminoethyl glycine and alkyl (C12-18) dimethyl benzyl glycine.
  • betaine surfactants such as dialkyl (C8-12) diaminoethyl betaine and alkyl (C12-18) dimethyl benzyl betaine
  • glycine surfactants such as dialkyl (C8-12) diaminoethyl glycine and alkyl (C12-18) dimethyl benzyl glycine.
  • the adjuvant is not particularly limited as long as it is one that can be used in ordinary agricultural and horticultural formulations, and may be, for example, one or more selected from the group consisting of carboxymethyl cellulose, hydroxypropyl cellulose, polyethylene glycol, gum arabic, polyvinylpyrrolidone, and starch.
  • the formulation form of the composition of the present invention is not particularly limited and may be any form that is commonly used for agricultural and horticultural chemicals, such as dust (DP, Dustable Powder), wettable powder (WP, Wattable Powder), emulsifiable concentrate (EC, Emulsifiable Concentrate), flowable (FL, Flowable), suspension concentrate (SC, Suspension Concentrate), water soluble powder (SP, Water Soluble Powder), water dispersible granule (WG, Water Dispersible Granule), tablet (Tablet), granule (GR, Granule), SE agent (Suspo Emulsion), OD agent (Oil Dispersion), EW agent (Emulsion oil in water), etc.
  • the method of preparation into the formulation is not particularly limited, and known preparation methods can be used depending on the dosage form. An example of a formulation is shown below. Note that "parts" means “parts by mass.”
  • Formulation 1 Wettable powder
  • 40 parts of the microorganism of the present invention, its culture, or a processed product of the culture, 53 parts of diatomaceous earth, 4 parts of a higher alcohol sulfate, and 3 parts of an alkylnaphthalene sulfonate are uniformly mixed and finely pulverized to obtain a wettable powder containing 40% of the active ingredient.
  • Formulation 2 Emulsion 30 parts of the microorganism of the present invention, its culture, or a processed product of the culture, 33 parts of xylene, 30 parts of dimethylformamide, and 7 parts of polyoxyethylene alkyl allyl ether are mixed and dissolved to obtain an emulsifiable concentrate containing 30% of the active ingredient.
  • Formulation 3 Granules
  • 5 parts of the microorganism of the present invention, its culture, or a processed product of the culture, 40 parts of talc, 38 parts of clay, 10 parts of bentonite, and 7 parts of sodium alkyl sulfate are uniformly mixed and finely pulverized, and then granulated into granules having a diameter of 0.5 to 1.0 mm to obtain a granule containing 5% of the active ingredient.
  • Formulation 4 Granules
  • 5 parts of the microorganism of the present invention, its culture, or a processed product of the culture, 73 parts of clay, 20 parts of bentonite, 1 part of dioctyl sulfosuccinate sodium salt, and 1 part of potassium phosphate are thoroughly ground and mixed, water is added, the mixture is thoroughly kneaded, and the mixture is granulated and dried to obtain granules containing 5% of the active ingredient.
  • Plant diseases and nematodes to which the composition of the present invention is applied applicable plants, application method, and non-agricultural uses
  • the plant diseases and nematodes to which the composition of the present invention is applied, the applicable plants, the application method, and non-agricultural uses are the same as the plant diseases and nematodes to which the microorganism of the present invention is applied, the applicable plants, the application method, and non-agricultural uses described above.
  • the second method of the present invention is a method for controlling plant diseases in post-harvest plants, which comprises treating post-harvest plants with the microorganism of the present invention, a culture thereof, a processed product of the culture, or a composition containing any of them (i.e., one or more selected from the group consisting of the microorganism of the present invention, a culture thereof, and a processed product of the culture).
  • a third method of the present invention is a method for controlling nematodes, comprising treating a plant and/or the soil in which the plant is grown with a composition containing the microorganism of the present invention, a culture thereof, a treated product of the culture, or any of them (i.e., one or more selected from the group consisting of the microorganism of the present invention, a culture thereof, and a treated product of the culture).
  • a fourth method of the present invention is a method for producing a plant, comprising treating a plant and/or the soil in which the plant is grown with a microorganism of the present invention, a culture thereof, a processed product of the culture, or a composition containing any of them (i.e., one or more selected from the group consisting of the microorganism of the present invention, a culture thereof, and a processed product of the culture).
  • the first method of the present invention, the second method of the present invention, the third method of the present invention, and the fourth method of the present invention may be collectively referred to as the "method of the present invention."
  • the method of the present invention comprises treating a plant and/or the soil in which the plant is grown with one or more selected from the group consisting of the following (1) to (5):
  • the second method of the present invention comprises treating a post-harvest plant with one or more selected from the group consisting of the following (1) to (5): (1) Bacillus sp. 201106_1 strain. (2) A mutant strain of the above (1), which has the ability to control plant diseases or nematodes. (3) A culture of the strain (1) or (2) above. (4) A processed product of the culture of (3) above. (5) A composition containing one or more selected from the group consisting of (1) to (4) above.
  • the term "plants and/or plant cultivation soil” encompasses the meanings of "plants and plant cultivation soil” and "plants or plant cultivation soil.” In other words, it means either or both of plants and plant cultivation soil.
  • the first method of the present invention, the third method of the present invention, and the fourth method of the present invention are not particularly limited, so long as they involve treating plants and/or plant cultivation soil with the microorganism of the present invention, a culture thereof, a processed product of the culture, or a composition containing any of them.
  • the second method of the present invention is not particularly limited, so long as it involves treating harvested plants with the microorganism of the present invention, a culture thereof, a processed product of the culture, or a composition containing any of them.
  • the mode of treatment may be selected appropriately depending on the type of plant disease or nematode, the type of plant to be applied, etc.
  • the microorganism of the present invention may be used without any particular limitation on the treatment method.
  • plants may be treated by directly applying or spraying the microorganism to the plant body, or the soil in which the plant is grown (plant cultivation soil) may be treated by mixing, spraying, or irrigating the soil.
  • planting soil planting soil
  • the soil may be treated before planting the plant, or the soil may be treated after planting the plant.
  • the microorganism of the present invention its culture, a treated product of the culture, or a composition containing any of them may be placed near the air outlet of an air blower that blows air into a facility, and sprayed together with the air blown out from the air outlet.
  • plant seeds, tubers, etc. may be coated, dusted, immersed, etc. with the microorganism of the present invention, its culture, a treated product of the culture, or a composition containing any of them, or the roots of plant seedlings may be treated by immersion.
  • a culture thereof, a treated product of the culture, or a composition containing any of them When treating plants and/or plant cultivation soil with the microorganism of the present invention, a culture thereof, a treated product of the culture, or a composition containing any of them, the microorganism of the present invention, a culture thereof, a treated product of the culture, or a composition containing any of them may be used as is, or may be diluted with an appropriate amount of water, etc.
  • the amount of spray water may be, for example, 1,000 to 10,000 L / ha, but for example, when a culture of the microorganism of the present invention is diluted 1,000 to 2,000 times with water and used, the amount of spray water can be small (for example, 100 to 1,000 L / ha).
  • Signal transduction inhibitors (a) Signal transduction inhibitors: quinoxyfen, proquinazid; (b) MAP/histidine kinase inhibitors in osmotic signaling: fenpiclonil, fludioxonil, chlozolinate, iprodione, procymidone, and vinclozolin.
  • Cell membrane sterol biosynthesis inhibitors (a) Inhibitors of C14 demethylation in sterol biosynthesis: triforine, pyrifenox, pyrisoxazole, fenarimol, flurprimidol, nuarimol, imazalil, imazalil sulfate, oxpoconazole fumarate fumarate, pefurazoate, prochloraz, triflumizole, viniconazole, azaconazole, bitertanol, bromuconazole, cyproconazole, diclobutrazol, difenoconazole, diniconazole, diniconazole-M, epoxyconazole, etaconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, fluconazole zole, fluconazole-cis, hexaconazole, imibenconazole, ipconazole, met
  • insecticides include miticides, nematicides, soil pesticides, anthelmintics, etc. that may be mixed or used in combination with the microorganism of the present invention, a culture thereof, a processed product of the culture, or a composition containing any of them are listed below.
  • Nicotinic acetylcholine receptor agonists acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, nithiazine, thiacloprid, thiamethoxam, sulfoxaflor, nicotine, flupyradifurone.
  • Nicotinic acetylcholine receptor allosteric modulators spinetoram, spinosad.
  • Chloride channel activators abamectin, emamectin-benzoate, lepimectin, milbemectin, ivermectin, selamectin, doramectin, eprinomectin, moxidectin, milbemycin, milbemycin oxime, and nemadectin.
  • Juvenile hormone-like substances hydroprene, kinoprene, methoprene, fenoxycarb, pyriproxyfen, diofenolan, epofenonane, triprene.
  • Non-specific inhibitors methyl bromide, chloropicrin, sulfuryl fluoride, borax, tartar emetic.
  • Homoptera-selective antifeedants flonicamid, pymetrozine, pyrifluquinazon.
  • Mite growth inhibitors clofentezine, diflovidazin, hexythiazox, etoxazole.
  • Microbial-derived insect midgut membrane disrupting agents Bacillus thuringiensis subsp. israelensi, Bacillus sphaericus, Bacillus thuringiensis subsp. aizawai, Bacillus thuringiensis subsp. kurstaki, Bacillus thuringiensis subsp.
  • a homology search was performed using BLAST on the partial nucleotide sequence of the phoR gene of the 201106_1 strain. The results confirmed that it exhibited high identity of over 99% with multiple strains belonging to Bacillus amyloliquefaciens or Bacillus veresensis, and in particular, 100% identity with two strains belonging to Bacillus veresensis.
  • Bacillus bacteria Commercially available pesticides containing Bacillus bacteria as an active ingredient include, for example, Agrocare Wettable Powder (Nisso Green), Serenade ASO (Bayer Crop Science), Botkiller Wettable Powder (SDS Biotech), Ecoshot (Kumiai Chemical Industry Co., Ltd.), and Impression Clear (SDS Biotech). Strains contained in these products were isolated, and the partial nucleotide sequence of the phoR gene was determined using the same method as above and compared with the partial nucleotide sequence of the phoR gene of strain 201106_1. As a result, no strains had a partial nucleotide sequence of the phoR gene that matched that of strain 201106_1.
  • Bacillus subtilis HAI-0404 the active ingredient in Agrocare Wettable Powder, showed the highest identity (99.5%). Therefore, in each of the tests in Examples 5 to 7 described below, the Bacillus subtilis HAI-0404 strain was used as a comparative example.
  • dam gene The nucleotide sequence of the dam gene of the 201106_1 strain was analyzed. Specifically, genomic DNA was first isolated from the 201106_1 strain according to standard methods. Using the obtained genomic DNA as a template, PCR amplification was performed according to standard methods using the dam-F primer (SEQ ID NO: 7) and the dam-R primer (SEQ ID NO: 8). The presence or absence of a PCR product was confirmed by agarose gel electrophoresis, and the obtained PCR product was sequenced using the dam-F primer or the dam-R primer to determine the partial nucleotide sequence of the dam gene of the 201106_1 strain (SEQ ID NO: 9).
  • a homology search was performed using BLAST on the partial nucleotide sequence of the dam gene of strain 201106_1.
  • the results showed high identity with multiple strains belonging to Bacillus amyloliquefaciens or Bacillus veresensis, but no strains with 98.0% or more identity were identified.
  • the complete genome sequences of two strains belonging to Bacillus veresensis with which the partial nucleotide sequence of the phoR gene showed 100% identity were examined, it was confirmed that neither of these two strains possessed the dam gene.
  • strain 201106_1 100 mL of standard liquid medium (0.25% (w/v) yeast extract, 0.5% (w/v) casein peptone, 0.1% (w/v) glucose, adjusted to pH 7.0 with sodium hydroxide) was placed in a 300 mL Erlenmeyer flask and sterilized by heating. The 201106_1 strain was inoculated and cultured in a reciprocating shaker at 30°C and 100 rpm for 3 days to obtain a culture.
  • standard liquid medium 0.25% (w/v) yeast extract, 0.5% (w/v) casein peptone, 0.1% (w/v) glucose, adjusted to pH 7.0 with sodium hydroxide
  • Example 2 [Test on the effectiveness of 201106_1 strain in controlling gray mold disease on beans (indoor test)] The culture of Example 2 was centrifuged at 10,000 x g for 5 minutes, and the supernatant was removed to obtain a culture precipitate containing primarily strain 201106_1. The precipitate was adjusted to an OD600 of 0.1 with ion-exchanged water, and kidney bean vases were immersed in the precipitate for 10 minutes. The kidney bean vases were then placed on a Petri dish lined with filter paper and air-dried for approximately 2 hours. A conidial suspension of Botrytis cinerea (5.0 x 10 spores/mL) was sprayed onto the kidney bean vases using an airbrush at a rate of 200 L/10 a.
  • Botrytis cinerea 5.0 x 10 spores/mL
  • kidney bean vases were then placed on the surface of cucumber cotyledons contained in a lidded plastic container for moisture retention and allowed to stand at 20°C in the dark and under moist conditions for 5 days.
  • the kidney bean vases and cucumber cotyledons were used for the vases and cucumber cotyledons, respectively, to facilitate testing.
  • a test was conducted in the same manner except that the strain used was Bacillus subtilis HAI-0404.
  • the diameter of the lesions formed on the cucumber cotyledons was measured.
  • the control value was calculated based on the following formula.
  • the "average" is the average of four replicates.
  • the extent of mycelial growth in the wells was visually inspected.
  • the filtrate was diluted 2-fold, 4-fold, 8-fold, and 16-fold with sterilized water and tested, and the maximum dilution rate at which the minimum inhibitory concentration was obtained was calculated.
  • a test was conducted in the same manner except that the strain used was Bacillus subtilis HAI-0404.
  • the maximum dilution rate of the culture supernatant of the 201106_1 strain for the minimum inhibitory concentration against spores of Botrytis cinerea was 8 times, which was superior to the 4 times of the Bacillus subtilis HAI-0404 strain.
  • the microorganisms of the present invention, their cultures, or culture supernatants that are processed products of the cultures have the ability to control at least Chinese cabbage soft rot as a bacterial disease, and at least the filamentous fungal diseases of bean gray mold, cucumber powdery mildew, broccoli downy mildew, peach brown rot, and citrus green mold as fungal diseases.
  • the microorganisms of the present invention, their cultures, or processed products of the cultures have a high level of plant disease control ability.
  • the microorganism, its culture, or a treated product of the culture of the present invention can effectively control plant diseases. Furthermore, because the microorganism, its culture, or a treated product of the culture of the present invention has high control activity against a variety of plant diseases, it is expected that it can be effectively used to control not only the above-mentioned Chinese cabbage soft rot, kidney bean gray mold, cucumber powdery mildew, broccoli downy mildew, peach brown rot, and citrus green mold, but also various other plant diseases such as gray mold, sclerotinia rot, powdery mildew, anthracnose, ring spot, blue mold, green mold, soft rot, and scab of various other plants.
  • the culture of the microorganism of the present invention has the ability to control peach brown rot and citrus green mold in post-harvest fruit, it is expected that the microorganism of the present invention, its culture, or a processed product of the culture can be effectively used to control various plant diseases not only in pre-harvest plants and/or plant cultivation soil, but also in post-harvest plants. Furthermore, since the culture of the microorganism of the present invention caused symptoms of paralysis in nematodes, it is expected that the microorganism of the present invention, its culture, or a processed product of the culture can be effectively used not only to control plant diseases but also to control nematodes.
  • SEQ ID NO: 1 9F primer GAGTTTGATCCTGGCTC
  • SEQ ID NO: 2 1500R primer TACCTTGTTACGACTT
  • SEQ ID NO: 4 phoR-F primer GTCCGTGTGCGTCTGTTCGC
  • SEQ ID NO: 5 phoR-R primer GCCGCTTCAGCGTCACG
  • SEQ ID NO: 9 Partial nucleotide sequence of the dam gene of strain 201106_1

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Agronomy & Crop Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention aborde le problème de la fourniture d'un micro-organisme dont la charge sur l'environnement est moindre, qui a une excellente capacité de lutte contre diverses maladies des plantes, et peut être efficacement utilisé pour la lutte contre des maladies des plantes et analogues sous la forme de cellules, d'un produit de culture des cellules, ou d'un surnageant de culture contenu dans le produit de culture.　Le problème peut être résolu à l'aide de la souche Bacillus sp. 201106_1 (souche NITE BP-03884).
PCT/JP2025/004054 2024-02-15 2025-02-07 Micro-organisme ayant la capacité à lutter contre les maladies des plantes Pending WO2025173649A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2024021425 2024-02-15
JP2024-021425 2024-02-15

Publications (1)

Publication Number Publication Date
WO2025173649A1 true WO2025173649A1 (fr) 2025-08-21

Family

ID=96772979

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2025/004054 Pending WO2025173649A1 (fr) 2024-02-15 2025-02-07 Micro-organisme ayant la capacité à lutter contre les maladies des plantes

Country Status (1)

Country Link
WO (1) WO2025173649A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120775754A (zh) * 2025-09-11 2025-10-14 广东省林业科学研究院 一种防治油茶炭疽病的芽孢杆菌及其应用
CN120905095A (zh) * 2025-10-10 2025-11-07 山东滨农科技有限公司 一株贝莱斯芽孢杆菌菌株jz01、包含其的菌剂及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016000031A (ja) * 2014-05-16 2016-01-07 シージェイ チェイルジェダン コーポレイション 新規なバチルス・ベレゼンシスｃｊｂｖ及びそれを含む抗菌組成物
JP2018508472A (ja) * 2014-12-29 2018-03-29 エフ エム シー コーポレーションＦｍｃ Ｃｏｒｐｏｒａｔｉｏｎ バチルス・アミロリケファシエンス（ｂａｃｉｌｌｕｓ ａｍｙｌｏｌｉｑｕｅｆａｃｉｅｎｓ）ｒｔｉ４７２組成物および植物成長に利益を与えかつ植物病害を処置するための使用方法
JP2023530740A (ja) * 2020-06-17 2023-07-19 バイオコンソーティア・インコーポレイテッド 農業的に有益な微生物、微生物組成物、及び共同体

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016000031A (ja) * 2014-05-16 2016-01-07 シージェイ チェイルジェダン コーポレイション 新規なバチルス・ベレゼンシスｃｊｂｖ及びそれを含む抗菌組成物
JP2018508472A (ja) * 2014-12-29 2018-03-29 エフ エム シー コーポレーションＦｍｃ Ｃｏｒｐｏｒａｔｉｏｎ バチルス・アミロリケファシエンス（ｂａｃｉｌｌｕｓ ａｍｙｌｏｌｉｑｕｅｆａｃｉｅｎｓ）ｒｔｉ４７２組成物および植物成長に利益を与えかつ植物病害を処置するための使用方法
JP2023530740A (ja) * 2020-06-17 2023-07-19 バイオコンソーティア・インコーポレイテッド 農業的に有益な微生物、微生物組成物、及び共同体

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120775754A (zh) * 2025-09-11 2025-10-14 广东省林业科学研究院 一种防治油茶炭疽病的芽孢杆菌及其应用
CN120905095A (zh) * 2025-10-10 2025-11-07 山东滨农科技有限公司 一株贝莱斯芽孢杆菌菌株jz01、包含其的菌剂及其应用

Similar Documents

Publication Publication Date Title
US12285017B2 (en) Bacillus amyloliquefaciens FCC1256 compositions and methods of controlling plant pathogens
USRE49311E1 (en) Bacillus thuringiensis RTI545 compositions and methods of use for benefiting plant growth and controlling plant pests
US10375964B2 (en) Microbial compositions and methods of use for benefiting plant growth and treating plant disease
JP6748735B2 (ja) 植物病害の防除能を有する微生物
US20190216091A1 (en) Bacillus licheniformis rti184 compositions and methods of use for benefiting plant growth
US20160186273A1 (en) Bacillus amyloliquefaciens rti301 compositions and methods of use for benefiting plant growth and treating plant disease
US20240196908A1 (en) Compositions and methods for improving plant health and controlling plant disease
EP3059304B1 (fr) Nouveau microorganisme et son utilisation
WO2025173649A1 (fr) Micro-organisme ayant la capacité à lutter contre les maladies des plantes
RU2756530C1 (ru) Композиция сельскохозяйственного и садового фунгицида
WO2020069297A1 (fr) Compositions de bacillus amyloliquefaciens fcc1256 et procédés de régulation d'agents pathogènes des végétaux
WO2020064480A1 (fr) Mélange pesticide comprenant un composé mésoionique et un biopesticide
JP7273199B2 (ja) 農園芸用組成物
KR20220012879A (ko) 식물 기생성 선충 방제제 및 식물 기생성 선충의 방제 방법
WO2025018374A1 (fr) Dérivé d'acide acrylique, et application associée
WO2025018373A1 (fr) Composé méthylènebisbiphényle, et application associée
WO2025018378A1 (fr) Composé biphényle, et application associée
WO2025018375A1 (fr) Composé diméthylpenta－２－ènedioate, et application associée
BR112022013133B1 (pt) Composição agrícola e hortícola, e, método para controlar doença de planta ou acelerar crescimento de planta

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 25754764

Country of ref document: EP

Kind code of ref document: A1