WO2025160618A1

WO2025160618A1 - A closeable bulk fluid assembly

Info

Publication number: WO2025160618A1
Application number: PCT/AU2025/050050
Authority: WO
Inventors: Pavel Dzitac
Original assignee: Leica Biosystems Melbourne Pty Ltd
Current assignee: Leica Biosystems Melbourne Pty Ltd
Priority date: 2024-01-31
Filing date: 2025-01-24
Publication date: 2025-08-07
Anticipated expiration: 2026-07-31

Abstract

Broadly speaking, the present disclosure relates to a closeable bulk fluid assembly ('CBA') which comprises a receptacle (e.g. bottle) configured to hold a reagent. The CBA may be configured to be connected to an instrument which uses the reagent (e.g. for medical purposes). In order to replenish the bottle of the CBA with a reagent, an operative scans the barcode of a reagent supply bottle. Consequently, a locking mechanism for the CBA is actuated and the front fascia will automatically open allowing access to the funnel of the bottle. The funnel can be easily opened by an operative. The operative may replenish the reagent in the bottle, but advantageously, the operative cannot remove the bottle from the CBA during a refill operation. Thus, human error is minimized because the chances of the operative mixing up the bottles are greatly reduced.

Description

A CLOSEABLE BULK FLUID ASSEMBLY

Technical Field

[0001] The present techniques relate to receptacles, and in particular, to closeable bulk fluid assemblies that contain a reagent, wherein the closeable bulk fluid assemblies are configured to be connected to an instrument, store the reagent, and supply the reagent from the closeable bulk fluid assembly to the instrument.

Background of Invention

[0002] Closeable bulk fluid assemblies ('CBAs') are a type of receptacle configured to hold a reagent. The reagent can be used for medical purposes. In the context of cancer treatment or medical treatment, a reagent refers to a substance or compound that is used to bring about a specific reaction or test. Reagents play a crucial role in various aspects of cancer research, diagnosis, and treatment. For instance, certain reagents may be used in diagnostic tests to identify the presence of specific biomarkers or cancer-related molecules. In another example, some reagents are employed directly in cancer treatment.

[0003] Oftentimes, reagents must be mixed at precise quantities in order to achieve the desired effect. This mixing of the reagents is performed in an instrument. Therefore, CBAs are also configured to be connected to such an instrument and to supply the reagent from the CBA to the instrument for mixing.

[0004] The mixing must be precise. For instance, if the mixing is not precise, a disease, such as cancer may be misdiagnosed. Therefore, it is desirable to know the amount of the reagent that has been dispensed from the CBA, and the amount of the reagent that remains in the CBA.

[0005] It is also desirable to provide a CBA in which the reagent can be easily and safely replenished.

[0006] The present applicant has recognized the above-mentioned or related shortcomings and the need to provide an improved CBA.

[0007] Any reference herein to a patent document or other matter which is given as prior art is not to be taken as an admission that the document or matter was known or that the information it contains was part of the common general knowledge as at the priority date of any of the claims. Summary of Invention

[0008] In one aspect, there is provided a closeable bulk fluid assembly, CBA, for holding a reagent and transferring the reagent from the CBA to an instrument, the CBA comprising: a bottle for holding the reagent; the bottle comprising a funnel, wherein the funnel is configured to move from a closed position to an open position; a fascia configured to move from a first fascia position to a second fascia position; wherein in the first fascia position, an operative is unable to access the funnel; and in the second fascia position, the operative is able to access the funnel and to move the funnel from the closed position to the open position to refill the bottle with the reagent but the operative is unable to remove the bottle out of the CBA.

[0009] The funnel may comprise at least one protrusion so that the operative may move the funnel from the closed position to the open position and back to the closed position with one finger.

[0010] The bottle may further comprise a tube connector, wherein the tube connector is configured to connect the bottle to the instrument.

[0011] The bottle may further comprise a coupling support and a bottle lock.

[0012] The CBA may further comprise a bottle support configured to support the bottle.

[0013] The bottle support may comprise two sloped wings for stabilizing the bottle in the bottle support.

[0014] The bottle support may be directly mounted on a load cell.

[0015] The fascia may be further configured to move to a third fascia position, wherein in the third fascia position, the bottle is removable from the CBA.

[0016] The fascia may comprise a pair of reinforcement ribs.

[0017] The fascia may comprise a fascia support to support the fascia so that the fascia is not in direct contact with the bottle.

[0018] One end of the fascia support may form a hinge mechanism so that the fascia is moveable from the first fascia position to the second fascia position and to the third fascia position.

[0019] The CBA may further comprise a locking mechanism for governing the movement of the fascia; wherein the locking mechanism comprises a locking bar. [0020] The locking bar may comprise a fascia adjustment cam; wherein the fascia adjustment cam is configured to align the fascia with the instrument.

[0021] The locking bar may comprise a first locking detection sensor, wherein the first locking detection sensor is moveable; wherein the locking mechanism comprises a second locking detection sensor; wherein the second locking detection sensor is fixed.

[0022] The first locking detection sensor and the second locking detection sensor may be parts of a reed switch.

[0023] The bottle support may comprise a protrusion; wherein the locking bar may comprise an overload protection cam rail; wherein the overload protection cam rail is configured to intake the protrusion when the fascia moves from the first fascia position to form an overload protection.

[0024] The locking mechanism may comprise a lock pin, a lever and a solenoid. The locking bar may further comprise at least two opening/closing grooves; wherein the lock pin is positioned on the lever; wherein the solenoid is configured to be actuated; wherein the lever is configured to move upon the actuation of the solenoid which moves the lock pin from a first pin position to a second pin position; and wherein in the first pin position, the lock pin is caught in a first of the at least two opening/closing grooves and in the second pin position, the lock pin is caught in a second of the at least two opening/closing grooves. In one or more examples, the locking mechanism may comprise a lock pin, a lever and a solenoid; the locking bar may further comprise a first lock pin depression and a second lock pin depression; wherein the lock pin is positioned on the lever; wherein the solenoid is configured to be actuated; wherein the lever is configured to move upon the actuation of the solenoid which moves the lock pin in a vertical direction; and when the lock pin is engaged with the first lock pin depression, the fascia is in the first fascia position and when the lock pin is engaged with the second lock pin depression, the fascia is in the second fascia position.

[0025] The locking bar may be configured to move in horizontal direction with the movement of the fascia.

[0026] The CBA may further comprise a bottle protection locking mechanism; wherein the bottle protection locking mechanism may comprise a bottle protection protrusion and a protection pin.

[0027] The CBA may further comprise a mechanical switch configured to sense the movement of the fascia. [0028] In another aspect, there is provided a system comprising: a closeable bulk fluid assembly, CBA, according to any one of the preceding statements; and an instrument configured to use the reagent.

[0029] The instrument may comprise a scanning means for scanning the reagent supply bottle; and wherein the instrument is configured to actuate the solenoid of the CBA.

[0030] The instrument may be an automated staining instrument.

[0031] A method of opening and closing the funnel of the bottle of the CBA for refilling the reagent is also provided herein. The method comprises scanning the reagent supply bottle for refilling. The method comprises initiating the fascia unlock sequence. The method comprises moving the fascia to an open fascia position. The method comprises sensing that the fascia has been opened. The method comprises detecting the movement of the fascia. The method comprises deactivating, by the instrument, the solenoid. The method comprises opening the funnel. The method comprises closing the funnel.

Brief Description of Drawings

[0032] Examples of the present techniques will now be described, by way of example only, with reference to the accompanying drawings in which:

[0033] Figure 1 shows a front perspective view of a first example of a CBA of the present techniques;

[0034] Figure 2 shows a side view of the CBA;

[0035] Figure 3 shows a perspective view of the CBA from the rear;

[0036] Figure 4 shows another side view of the CBA;

[0037] Figure 5 shows a side perspective view of the CBA;

[0038] Figure 6 shows another perspective view of the CBA from the rear;

[0039] Figure 7 shows a side perspective view of the CBA with an opened funnel;

[0040] Figure 8 shows another side perspective view of the CBA with the opened funnel;

[0041] Figure 9 shows a side view of the CBA with the opened funnel; [0042] Figure 10 shows another side view of the CBA with the opened funnel;

[0043] Figure 11 shows a side view of a locking mechanism of the CBA;

[0044] Figure 12 shows the opposite side view of the locking mechanism of the CBA of Figure 11;

[0045] Figure 13 shows a cross section of an overload protection cover of the CBA;

[0046] Figure 14 shows a side view of an overload protection of the CBA;

[0047] Figure 15 shows a side view of the CBA with fascia in an intermediate position;

[0048] Figure 16 shows a side view of the CBA with a fully open fascia in the service position (third fascia position);

[0049] Figure 17 shows an exploded view of a bottle of the CBA;

[0050] Figure 18 shows a perspective view of the funnel in a closed position;

[0051] Figure 19 shows a perspective view of the funnel in an open position;

[0052] Figure 20 shows a front-perspective view of the bottle of the CBA;

[0053] Figure 21 shows a perspective view of the bottle of the CBA from the rear;

[0054] Figure 22 shows a side view of the bottle of the CBA;

[0055] Figure 23 shows a perspective view of a bottle support;

[0056] Figure 24 shows another perspective view of the bottle support;

[0057] Figure 25 shows a side view of the bottle support;

[0058] Figure 26 shows a front perspective view of a second example of a CBA of the present techniques;

[0059] Figure 1 shows a side view of the second exemplary CBA;

[0060] Figure 28 shows a perspective view of the second exemplary CBA from the rear;

[0061] Figure 29 shows another side view of the second exemplary CBA; [0062] Figure 30 shows a side perspective view of the second exemplary CBA with an opened funnel;

[0063] Figure 31 shows a side view of the second exemplary CBA with an open funnel;

[0064] Figure 32 shows a side view of the second exemplary CBA with a fascia in an intermediate position;

[0065] Figure 33 shows a side view of the second exemplary CBA with a fully open fascia in the service position (third fascia position);

[0066] Figure 34 shows an exploded view of the bottle and the funnel of the second exemplary CBA;

[0067] Figure 35 shows a perspective view of the funnel of the second exemplary CBA in the closed position;

[0068] Figure 36 shows a side view of the locking mechanism of the second exemplary CBA;

[0069] Figure 37 shows the opposite side view of the locking mechanism of the second exemplary

CBA;

[0070] Figure 38 shows a cross section view of an overload base of the second exemplary CBA;

[0071] Figure 39 shows a side view of an overload protection of the second exemplary CBA;

[0072] Figure 40 a side view of a bottle protection locking mechanism when fascia is unlocked in the service position;

[0073] Figure 41 shows a side view of the bottle protection locking mechanism when the fascia is in the closed position;

[0074] Figure 42 shows a perspective view of a bottle support of the second exemplary CBA;

[0075] Figure 43 shows another perspective view of a bottle support of the second exemplary

CBA;

[0076] Figure 44 shows a side view of the bottle support of the second exemplary CBA;

[0077] Figure 45 shows a front perspective view of a third example of a CBA of the present techniques; [0078] Figure 46 shows a side view of the third exemplary CBA;

[0079] Figure 47 shows a perspective view of the third exemplary CBA from the rear;

[0080] Figure 48 shows another side view of the third exemplary CBA;

[0081] Figure 49 shows another perspective view of the third exemplary CBA;

[0082] Figure 50 shows another perspective view of the third exemplary CBA from the rear;

[0083] Figure 51 shows a side perspective view of the third exemplary CBA with open funnel;

[0084] Figure 52 shows a side view of the third exemplary CBA with open funnel;

[0085] Figure 53 shows a side view of the third exemplary CBA with a fully open fascia in the service position (third fascia position);

[0086] Figure 54 shows a side view of the locking mechanism of the third exemplary CBA;

[0087] Figure 55 shows the opposite side view of the locking mechanism of the third exemplary

CBA;

[0088] Figure 56 shows a cross-section view of an overload base of the third exemplary CBA;

[0089] Figure 57 shows a side view of an overload protection of the third exemplary CBA;

[0090] Figure 58 shows an exploded view of a bottle of the third exemplary CBA;

[0091] Figure 59 shows a perspective view of the funnel in a closed position;

[0092] Figure 60 shows a perspective view of the funnel in an open position;

[0093] Figure 61 shows a side view of the bottle of the third exemplary CBA;

[0094] Figure 62 shows a perspective view of a bottle support of the third exemplary CBA;

[0095] Figure 63 shows another perspective view of a bottle support of the third exemplary CBA;

[0096] Figure 64 shows a side view of the bottle support of the third exemplary CBA;

[0097] Figure 65 shows a method of refilling the bottle of the CBAs of the present techniques; and [0098] Figure 66 shows a schematic diagram of a system comprising an instrument and the CBAs of the present techniques.

Detailed Description

[0099] Broadly speaking, the present disclosure relates to a closeable bulk fluid assembly ('CBA') which comprises a receptacle (e.g. bottle) configured to hold a reagent. The CBA may be configured to be connected to an instrument which uses the reagent (e.g. for medical purposes). In order to replenish the bottle of the CBA with a reagent, an operative scans the barcode of a reagent supply bottle. Consequently, a locking mechanism for the CBA is actuated and the front fascia will automatically open allowing access to the funnel of the bottle. The funnel can be easily opened by an operative. The operative may replenish the reagent in the bottle, but advantageously, the operative cannot remove the bottle from the CBA during a refill operation. Thus, human error is minimized because the chances of the operative mixing up the bottles are greatly reduced.

[0100] The features which are the same or substantially similar retain the same reference numbers in the Figures and throughout the description. Therefore, a repeated description of such features is omitted.

[0101] All examples of the present techniques may be combined together, unless it is explicitly stated otherwise.

[0102] Figures 1 to 6 show different views of the CBA 1 of the present techniques with a closed funnel 320. Figures 7 to 10 show different views of the CBA 1 with an open funnel 320.

[0103] The CBA 1 comprises a front fascia 100, a bottle 300 with a funnel 320, and a locking mechanism.

[0104] The CBA 1 is configured to hold a reagent in the bottle 300. The CBA 1 is also configured to be releasably connected to an instrument (not shown) that can use and/or process the reagent. The reagent may be stored in the bottle 300 in a liquid form.

[0105] The bottle 300 is shown in greater detail in Figures 17 to 22.

[0106] The bottle 300 is a receptacle that is configured to hold a reagent (or any other liquid).

[0107] The bottle 300 comprises a funnel 320. The funnel 320 is configured to move from a closed position to an open position. When the funnel 320 is in the closed position, the bottle 300 cannot be refilled by an operative (i.e. the bottle 300 is closed). When the funnel 320 is in the open position, the bottle 300 can be refilled by an operative (i.e. the bottle 300 is open). Beneficially, the funnel 320 allows the operative to replenish the reagent in the bottle 300, but prevents the operative from pulling (removing) the entire bottle 300 out of the CBA 1. Thus, human error is minimized because the chances of the operative mixing up the bottles are greatly reduced.

[0108] The bottle 300 may comprise two bottle hinge indents 326 (for example, as shown in Figure 17). The funnel 320 may comprise a funnel hinge bar 325. The two bottle hinge indents 326 are configured such that the funnel hinge bar 325 may be pushed between the bottle hinge indents 326. Thus, the funnel 320 may be moved from the closed position to an open position, and back, via the hinge-like motion around the funnel hinge bar 325. The skilled person will appreciate that any other similar hinge-like mechanism may be used for opening and closing the funnel 320. The funnel 320 may comprise sealing material in the vicinity of the hinge bar to prevent leakage during refilling.

[0109] The funnel 320 may comprise at least one opening protrusion 322. The at least one opening protrusion is configured to assist the operative in opening the funnel 320 more easily. For instance, the at least one opening protrusion may be in the form of a single hook 322 that is shaped in such a way that it fits a human finger. Accordingly, the operative may use only one finger to pull on the at least one opening protrusion 322 to move the funnel 320 from the closed position to an open position. The operative may use only one finger to move the funnel 320 back to the closed position. The operative may actuate the at least one opening protrusion 322 (or the funnel 320) to move the funnel 320 from an open position to a closed position.

[0110] The bottle 300 comprises a tube connector 302. The tube connector 302 is configured to connect the CBA 1 and an instrument. Thus, the transfer of the reagent from the bottle 300 of the CBA 1 to the instrument may be achieved.

[0111] The CBA 1 may comprise a coupling support 306. The bottle 300 may comprise a bottle lock 304.

[0112] The bottle lock 304 may comprise two tabs that latch onto the coupling support 306 and hold the bottle 300 in a steady place. Thus, beneficially, the bottle 300 may be secured in the bottle support 308 preventing unintended movement during use of the instrument. The coupling support 306 may be coupled with the bottle 300 via the bottle lock 304. The coupling support 306 also supports the tube connector 302 and holds the bottle 300, and the CBA 1, in a steady place so that a firm and stable connection with the instrument may be achieved. The coupling support 306 may be releasably anchored in the bottle support 308. For instance, the coupling support 306 may be inserted between the two tabs 304. The coupling support 306 may comprise an opening 307 that facilitates the tube connector 302. The opening 307 may have a fork-like shape. Beneficially, the coupling support 306 also supports the stability of the tube connector 302 when the tube connector 302 is connected to the instrument. The coupling support 306 may further comprise a connector bump (not shown). The connector bump is positioned in the opening 307 of the coupling support 306 so that when the tube connector 302 is inserted into the opening 307, the connector bump helps to stabilize the tube connector 302 in the coupling support 306.

[0113] The CBA 1 may comprise a bottle support 308 (for example, shown in Figures 23 to 25). The bottle support 308 is configured to support the bottle 300. The bottle support 308 comprises an intake area 309. The bottle 300 is inserted into the intake area 309. The bottle support 308 comprises two wings 310 to stabilize the bottle 300 in the intake area 309. The two wings 310 may be sloped for more secure attachment of the bottle 300. The bottle support 308 may comprise bottle stabilizing means 313 (shown in Figure 42). The bottle stabilizing means 313 may be configured to keep the bottle 300 in the desired place on the bottle support 308 (e.g. for a more precise alignment with the load cell 203). The bottle stabilizing means 313 may comprise at least one protrusion/indent. The at least one protrusion/ident may be a mating indent. The bottle support 308 may comprise coupling anchoring means 316. The coupling anchoring means 316 may be configured to releasably anchor the coupling support 306 to the bottle support 308. The coupling anchoring means 316 may comprise at least one mating indent and/or at least one mating hole.

[0114] The bottle 300 may comprise a sloped bottle feature 333. The sloped bottle feature 333 may be shaped in such a way that the two wings 310 can embrace the bottle 300 more tightly, thus, the bottle 300 is more stable in the bottle support 308. Therefore, advantageously, the bottle support 308 may prevent the bottle 300 from disconnecting due to vibrations.

[0115] The bottle support 308 comprises a mounting portion 312. The mounting portion 312 enables the bottle 300 and the bottle support 308 to be mounted directly on a load cell. For instance, the mounting portion 312 may be mounted to the load cell 203 via screws (e.g. two screws). In other words, the centre of mass of the bottle 300 is correctly aligned with the load cell 203, within the load cell capability. Thus, accurate weight measurements of the bottle 300 may be achieved. This is important because the load cell 203 may measure the weight of the bottle 300 and thus the amount of reagent that was dispensed from the bottle 300 after every use. This information may be used by the operative to refill the bottle 300 at appropriate times. This information may be also used by the operative to order reagent when the reagent is running low in stock. This information may also be processed by the instrument. For instance, the instrument may display the information to the operative and/or automatically order a reagent. [0116] The bottle 300 may have a capacity between IL to 10L. In some examples, the capacity of the bottle 300 is 1.5L. For instance, the bottle 300 may be made of polyethylene. The bottle support 308 may be made of POM-C or POM-H. The skilled person will appreciate that other suitable materials may be used.

[0117] The CBA 1 comprises a front fascia 100. Beneficially, the front fascia 100 prevents dust and other undesirable particles from getting into the CBA 1. Also beneficially, the front fascia 100 prevents unauthorized access to the funnel 320, therefore, the security of the CBA 1 is enhanced.

[0118] During a 'refill' operation, the front fascia 100 is configured to move from a closed position ('first fascia position') to an open position ('second fascia position'). During a 'service' operation, the front fascia 100 may be moved from the closed position ('the first fascia position') to a service position ('third fascia position'). During a 'service' operation, the front fascia 100 may be moved between the closed position ('the first fascia position') and a service position ('third fascia position'), travelling through a plurality of intermediate positions (as for example shown by the bold arrow in Figure 15). The intermediate positions are positions of the front fascia 100 between the first fascia position and the third fascia position during the service operation. In the intermediate position and the service position the funnel 320 is closed. The front fascia 100 is configured to move automatically upon receiving a signal from the instrument.

[0119] When the fascia 100 is in the closed position (first fascia position), an operative is unable to access the funnel 320. When the fascia 100 is in an open position (second fascia position), an operative is able to access the funnel 320, but the operative is unable to take the bottle 300 out of the CBA 1. When the fascia 100 is in the service position (the third fascia position), the operative is able to take out the entire bottle 300 out of the CBA 1. In the first and second fascia positions, the instrument may be in operation and/or connected to the CBA 1. In the third fascia position, the instrument is not in operation. Alternatively the instrument may be in operation using a reagent bottle other than the reagent bottle whose a fascia in the third fascia position.

[0120] In this scenario, the closed position means that the fascia 100 is substantially parallel with the nearest side of the bottle 300. The fascia 100 is configured to be rotated from a substantially vertical position (closed position) in a horizontal direction via a hinge-like motion. The hinge-like motion may be achieved by a fascia hinge 405 formed between the fascia support 102 and the base 400. The skilled person will appreciate that other similar hinge mechanisms may be used.

[0121] It will be appreciated that the fascia 100 does not have to be fully moved to the substantially horizontal position for an operative to access the funnel 320. The nominal fill position for the operative to access the funnel 320 may be 17 degrees in the horizontal direction from the closed position. Even when the fascia 100 is moved between 15 to 19 degrees in the horizontal direction from the closed position, an operative may access the funnel 320. In the first fascia position, the fascia 100 is substantially parallel to the nearest side of the bottle 300. In other words, in the first fascia position, the fascia 100 is substantially perpendicular to the base 400. In the second fascia position, the fascia is approximately between 10 to 30 degrees from the nearest side of the bottle 300. In the second fascia position, the fascia 100 is preferably approximately between 15 to 19 degrees from the nearest side of the bottle 300. The skilled person will appreciate that in the second fascia position, the fascia 100 must be far away enough from the funnel 320 so that the operative may access the funnel 320. In the third fascia position, the fascia 100 is substantially perpendicular to the nearest side of the bottle. In other words, in the third fascia position, the fascia 100 is in a substantially horizontal position, substantially parallel to the base 400.

[0122] The fascia 100 may comprise a pair of reinforcement ribs 104. The reinforcement ribs 104 may protrude along the inner edges of the fascia 100. The reinforcement ribs 104 may be tapered. Beneficially, the reinforcement ribs 104 may support the rigidity of the fascia 100.

[0123] The fascia 100 may further comprise at least one opening 106. The opening may be a capsule-shaped and/or rectangular opening. The bottle 300 may be wholly or partially transparent. Beneficially, the at least one opening 106 enables the operative to see the liquid level in the bottle 300.

[0124] The CBA 1 comprises a front fascia support 102. The front fascia support 102 supports the fascia 100. The fascia support 102 connects the fascia 100 to the locking mechanism. Thus, the fascia 100 is not in direct contact with the bottle 300 and this is advantageous because the weight of the bottle 300 is not affected by the weight of the fascia 100. This is also advantageous because the fascia 100 does not interfere with the bottle 300 or any other part of the weighting system. Thus, correct measurements of the weight of the bottle 300 and hence dispensed reagent from the bottle 300 may be achieved. Also beneficially, because the fascia 100 is not in direct contact with the bottle 300, the bottle 300 is not moved by the movement of the fascia 100 which may result, for instance, in excessive overloading of the load cell 203.

[0125] One end of the fascia support 102, which is not attached to the fascia 100, is arranged to form a fascia hinge 405 (hinge mechanism) between the fascia support 102 and the base 400 so that the fascia 100 is able to be rotated, in a hinge-like motion, from a substantially vertical position (closed position) in a horizontal direction, to a substantially horizonal position (third fascia position) and back. In an example, the fascia support 102 may comprise a fascia hinge bar positioned at one end of the fascia support 102 that is not attached to the fascia 100. The base 400 may comprise two fascia hinge holders which are configured such that the fascia hinge bar may be placed between the fascia hinge holders. Thus, the fascia 100 may be moved from the closed position to an open position (and to a service position), and back, via the hinge-like motion around the fascia hinge bar.

[0126] Figures 11 to 13 show the locking mechanism.

[0127] The CBA 1 comprises a locking mechanism. The locking mechanism is configured to govern the movement of the fascia 100.

[0128] The locking mechanism comprises a locking bar 204. The locking bar 204 is a multifunctional component that facilitates several components of the locking mechanism. For instance, a load cell overload protection may be positioned in the locking bar 204. A front fascia adjustment cam 202 is attached to the locking bar 204. The locking bar 204 may comprise dowel pins 219, 220. The locking bar 204 may further comprise an overload protection cam rail 236.

[0129] The locking bar 204 comprises a front fascia adjustment cam 202. The cam 202 is configured to act as a mechanical linkage between the locking bar 204 and the front fascia 100. The front fascia support 102 is attached to the locking bar 204 via the fascia adjustment cam 202. In an example, the front fascia support 102 has a substantially radial opening. The fascia adjustment cam 202 is configured to fit into the substantially radial opening of the fascia support 102. Beneficially, the cam 202 is configured to adjust the position of the front fascia 100 so that the fascia 100 can be properly aligned with respect to the instrument. Furthermore, the CBA may comprise a fascia compression spring 103. Beneficially, the cam 202 is configured to adjust the position of the front fascia 100 so that a correct alignment between the fascia 100 and its fascia compression spring 103 may be achieved. The compression spring 103 assists the movement of the fascia 100 from the closed to open position and back, therefore, a proper alignment between the fascia compression spring 103 and the fascia 100 results in a more mechanically efficient system. The fascia compression spring 103may be located under the fascia support 102 so that it can exert force on the fascia support 102. In this way, the fascia compression spring 103 may rotate the fascia support 102 to different fascia positions (e.g., first fascia position, second fascia position, third fascia position).

[0130] The CBA 1 comprises a load cell 203. The load cell 203 may be located between the base 400 and the bottle support 308. The load cell 203 may be directly secured to the base 400. For instance, two screws may be used to secure the load cell 203 to the base 400. The bottle support 308 may be directly secured on the load cell 203. The load cell 203 may not be in direct contact with the locking bar 204. The load cell 203 is configured to measure the weight of the bottle 300. [0131] The locking bar 204 comprises two dowel pins 219, 220. The two dowel pins 219, 220 are located horizontally in the locking bar 204. The dowel pins 219, 220 are configured to engage with a lock pin 210. The first dowel pin 219 is configured to hold the lock pin 210 and in this configuration the fascia 100 is in the first fascia position (closed position). The second dowel pin 220 is configured to hold the lock pin 210 and in this configuration the fascia 100 is in a second fascia position (open position and ready to be refilled). The lock pin 210 is configured to engage only the first dowel pin 219 or the second dowel pin 220. In other words, the lock pin 210 cannot engage both dowel pins 219,220 simultaneously. The lock pin 210 may not engage either one of the dowel pins 219, 220. The lock pin 210 may not engage either one of the dowel pins 219, 220 when the fascia 100 is in the third fascia position ('service' position).

[0132] The locking bar 204 may comprise a screw 226. The screw 226 is fitted substantially at one end of the locking bar 204. The head of the screw 226 at the end of the locking bar 204 is above the locking bar 204 which serves as a hard stop. By releasing the screw 226 at the end of the locking bar 204, the fascia 100 travel limit may be extended. Thus, the fascia 100 may travel to the and optionally past the third fascia position ('service position'). In one or more examples, the screw 226 may be replaced by a dowel pin.

[0133] The locking mechanism may comprise a first locking detection sensor 206. The first locking detection sensor 206 is attached to the locking bar 204. Accordingly, the first locking detection sensor 206 is moveable. The first locking detection sensor 206 may be magnetic (e.g. a reed switch). The skilled person will appreciate that the first locking detection sensor 206 may comprise one or more sensors.

[0134] The locking mechanism may comprise a second locking detection sensor 207. The second locking detection sensor 207 is fixed on the lever support 211. In other words, the second locking detection sensor 207 is not moveable. The second locking detection sensor 207 may be magnetic (e.g., a reed switch). The skilled person will appreciate that the second locking detection sensor 207 may comprise one or more sensors. In an example, the first locking detection sensor 206 and the second locking detection sensor 207 may be a part of the same sensor such as the reed switch.

[0135] In the art, usually, the first locking detection sensor 206 is moved away from the second locking detection sensor 207 along an imaginary y-axis (i.e., up and down). Undesirably, this results in the second locking detection sensor 207 sensing the first locking detection sensor 206 at large distances which may lead to less accurate measurements. In contrast, in the present application, beneficially, the first locking detection sensor 206 is configured to slide with the locking bar 204 (along the imaginary x- axis, from side to side of the locking bar 204), while the second locking detection sensor 207 is fixed on the lever support 211. Thus, the sliding movement of the first locking detection sensor 206 with respect to the fixed second locking detection sensor 207 results in shorter distance sensing and increased precision.

[0136] The locking mechanism may comprise an overload base 208. The overload base 208 comprises an opening for the locking bar 204. The locking bar 204 penetrates through the opening of overload base 208. The overload base 208 may be made of two parts so that it is easier to manufacture (e.g. via injection moulding). The two parts of the overload base 208 may be attached to each other via a snap fit attachment.

[0137] The locking mechanism comprises the lock pin 210 and a lever 212. The lock pin 210 is positioned in the overload base 208 on the lever 212. The lock pin 210 is configured to interact with the two dowel pins 219, 220 in the locking bar 204.

[0138] The locking mechanism may comprise a lever support 211. Lever support 211 is fixed (i.e. does not move) and supports the lever 212 which is moveable.

[0139] The locking mechanism may further comprise a lever spring 213. The lever spring 213 is configured to assist the movement of the lever 212. The lever spring 213 may be in contact with lever 212.

[0140] The locking mechanism comprises a locking solenoid 214. The solenoid 214 is configured to communicate with the instrument. In other words, the instrument may actuate the solenoid 214. Upon actuation, the solenoid 214 is configured to interact with the lever 212 which forces the movement of the lock pin 210.

[0141] The locking mechanism may comprise a solenoid frame 216 for holding the locking solenoid 214 in place. The solenoid frame 216 may comprise one or more openings (e.g. two) for wires that may lead to the CBA 1.

[0142] The CBA 1 may comprise a base 400. The base 400 is configured to contain all of the elements of the CBA 1. In other words, the components of the CBA 1 may be positioned on the base 400. The base 400 may comprise a flat bottom surface so that the CBA 1 may be stable on a flat surface. Beneficially, the bottom surface of the base 400 may comprise anti-slip means so that the base 400 is more stable on flat surfaces.

[0143] The CBA 1 may comprise an electronics circuit (not shown). The CBA 1 may further comprise an electronics circuit cover 222. The electronics circuit may calibrate the load cell 203. The electronics circuit may measure the weight of the bottle 300. In other words, the electronics circuit may be in communication with the load cell 203 and/or the instrument. The electronics circuit may be housed in the electronics circuit cover 222. Beneficially, the electronics circuit cover 222 may protect the electronics circuit from unintentional spillage during refilling. The electronics circuit may comprise a PCB.

[0144] The locking mechanism may comprise an overload protection 230. Figure 14 shows the overload protection 230 of the CBA 1 in a greater detail.

[0145] The overload protection 230 comprises a protrusion 231. The protrusion 231 may be positioned at the bottom surface of the bottle support 308. The locking bar 204 may comprise an overload protection cam rail 236. The protection cam rail 236 may be formed as a depression configured to intake the protrusion 231 of the overload protection 230. The overload protection cam rail 236 is configured to intake the protrusion 231 when the fascia 100 moves from the first fascia position to form an overload protection.

[0146] When fascia 100 opens, the overload protection protrusion 231 moves into the protection cam rail 236. Thus advantageously, this prevents bottle support 308, which may be bolted on the load cell 203, from applying excessive load onto the load cell 203. This ensures that the load cell 203 will not be overloaded if the operative presses onto the bottle 300 or the bottle support 308 while fascia 100 is open. Thus, the load cell 203 is protected and is properly functional to measure the weight of the bottle 300. If the overload protection was not in place, the operative may damage the load cell 203 by applying force on the bottle support during refilling (e.g., leaning on the bottle) because of the short distances between the bottle support 308 and the load cell 203. In some instances, the distance between the bottle support 308 and the load cell may be only a few millimetres (e.g., 2.5 mm).

[0147] In one example, the load cell 203 has four strain gauges. The centre of mass of bottle 300 is positioned such that it is positioned between the strain gauges. This achieves a greater weighing accuracy. Therefore, advantageously, the bottle 300 may be precisely weighed. The load cell 203 may be configured to be in communication (e.g. via the electronics circuit) with the instrument (or any other device) and send the weight measurement to the instrument. Therefore, beneficially, the precise amount of reagent dispensed may be measured. Also beneficially, the amount of reagent left in the bottle 300 may be measured or ascertained.

[0148] Figures 26 to 44 show another example of a CBA 2. CBA 1 and CBA 2 are substantially similar; therefore, the following description of the CBA 2 focuses only on the major differences between CBA 1 and CBA 2. Furthermore, the features which are the same or substantially similar retain the same reference numbers in the Figures and throughout the description. Therefore, a repeated description of such features is omitted.

[0149] Furthermore, CBA 2 comprises a bottle protection locking mechanism. The bottle protection locking mechanism comprises a bottle protection protrusion 500 and a bottle protection pin 502.

[0150] The bottle protection protrusion 500 is located on the locking bar 204. The bottle protection pin 502 is located in the bottle support 308. The bottle protection pin 502 is configured to engage with the bottle protection protrusion 500.

[0151] To accommodate the bottle protection pin 502, the bottle support 308 may comprise a safety projection 506. To further accommodate the bottle protection pin 502, the bottle 300 may comprise a bottle protection recess 510.

[0152] The bottle protection recess 510 is configured to intake the bottle protection pin 502 via the safety projection 506. The bottle support 308 may further comprise a drain hole located close to the safety projection 506 so that any spillage may be captured by the drain hole. Thus, advantageously, any spillage does not interfere with the operation of the bottle protection pin 502.

[0153] The locking bar 204 moves in horizontal direction as the fascia 100 moves from the first fascia position to the second fascia position and also to the third fascia position and back. The movement of the locking bar 204 results in the movement of the safety protrusion 500 because the safety protrusion 500 is located on the locking bar 204. The bottle protection recess 510 is aligned with the safety projection 506 so that when the bottle protection pin 502 engages with the bottle protection protrusion 500, the bottle protection protrusion 500 forces the bottle protection pin 502 in an upward vertical direction through the safety projection 506 to the bottle protection recess 510. This occurs when the fascia 100 is in open position. In other words, this occurs when the lock pin 210 is engaged with the second dowel pin 220. Figure 40 shows a scenario when the fascia 100 is in the third fascia position ('service' position). Figure 41 shows a scenario when the fascia 100 is in the closed position. Therefore, advantageously, the bottle 300 does not interfere with the load cell 203 and the bottle 300 may be refilled without any damage to the load cell 203 caused by the operative. Further advantage is that when the fascia 100 is in open position, the bottle 300 is more stable, therefore, it is easier for the operative to refill the bottle 300. Overall, the bottle protection locking mechanism improves the sturdiness and usability of the CBA. [0154] The bottle support 308 may additionally comprise a backside wing 314. The backside wing

314 protects the CBA 2 against a leakage that can occur during bottle refilling.

[0155] The funnel 320 may additionally comprise an elongated flat surface 327. The elongated flat surface 327 may be located near the funnel hinge bar 325. Beneficially, the elongated flat surface 327 prevents leakage when the funnel 320 is open and the bottle 300 is being refilled.

[0156] The funnel 320 may additionally comprise a sealing material. The sealing material may be located on the inside of the funnel near the funnel hinge bar 325. Thus, advantageously, the sealing material prevents leakage when the funnel 320 is open and the bottle 300 is being refilled.

[0157] CBA 2 may comprise a mechanical switch 2060. The skilled person will appreciate that the mechanical switch 2060 may be used as an alternative instead of the first locking detection sensor 206 and the second locking detection sensor 207. The function of the mechanical switch 2060 is the same as the function of the first and the second locking detection sensors 206, 207. In other words, the mechanical switch 2060 is configured to sense that the fascia 100 has been opened. In other words, the mechanical switch 2060 is configured to sense whether the fascia 100 is in the closed position. The mechanical switch 2060 may sense that the fascia 100 has been opened due to the movement of the locking bar 204. The mechanical switch 2060 is configured to communicate with the instrument. The mechanical switch 2060 may communicate with the instrument that the fascia 100 has changed position.

[0158] Figures 45 to 64 show another example of a CBA 3. CBA 1, CBA 2 and CBA 3 are substantially similar; therefore, the following description of the CBA 3 focuses only on the major differences between CBA 3 and CBA 1 and/or CBA 2. Furthermore, the features which are the same or substantially similar retain the same reference numbers in the Figures and throughout the description. Therefore, a repeated description of such features is omitted.

[0159] In the third exemplary CBA 3, the fascia 100 and the fascia support 102 are combined into a single part. In other words, the fascia 100 and the fascia support 102 may be integral with each other. Thus, beneficially, reliability of the CBA is increased. Further, beneficially, the CBA 3 is simpler to assemble and manufacture.

[0160] In the third exemplary CBA 3, the overload base 208 further comprises a mechanical switch area. The mechanical switch area may comprise at least one indent. The mechanical switch 2060 may be attached to the overload base 208. For instance, the mechanical switch 2060 may be attached to the overload base 208 via a snap fit attachment. This makes the overall design of the CBA 3 more compact.

[0161] In the third exemplary CBA 3, the locking bar 204 may comprise two lock pin depressions 239, 240 (for example, shown in Figure 57) instead of the dowel pins 219, 220. The two lock pin depressions 239, 240 are configured to interact with the lock pin 210 in a similar manner as the dowel pins 219,220.

[0162] In some examples, the locking mechanism comprises a lock pin, a lever and a solenoid; the locking bar further comprises a first lock pin depression and a second lock pin depression; wherein the lock pin is positioned on the lever; wherein the solenoid is configured to be actuated; wherein the lever is configured to move upon the actuation of the solenoid which moves the lock pin in a vertical direction; and when the lock pin is engaged with the first lock pin depression, the fascia is in the first fascia position and when the lock pin is engaged with the second lock pin depression, the fascia is in the second fascia position. The locking bar may comprise more than two lock pin depressions.

[0163] In the third exemplary CBA 3, the bottle support 308 may further comprise a drainage hole 319. The drainage hole 319 is configured to drain any accidental spillage. Furthermore, the drainage hole 319 may be sized so that a screwdriver may fit through the drainage hole 319. Thus, beneficially, easier maintenance of the CBA may be achieved.

[0164] In the third exemplary CBA 3, the bottle 300 may comprise at least one rib at the bottom surface of the bottle 300 (i.e. the surface that is in contact with the bottle support 308 in use). Beneficially, the at least one rib stabilizes the bottle 300 in the bottle support 308.

[0165] In the third exemplary CBA 3, the front face 328 of the funnel 320 is angled inwards. The angle a is shown in Figure 59. The angle a may be about 17 degrees. In some examples, the angle a may be between 10 to 30 degrees. In some examples, the angle a may be between 30 to 50 degrees. Beneficially, this prevents leakage outside of the bottle 300 during refilling.

[0166] The funnel 320 may further comprise a sharp edge 329. In some examples, the funnel 320 may further comprise two sharp edges 329. In some examples, the funnel 320 may comprise more than two sharp edges 329. It will be appreciated that the bottle 300 is configured to hold any type of a reagent. Different reagents have different surface tensions. Reagents with differing surface tensions may exhibit capillary action or creep along the surface, potentially leading to upward migration from the funnel 320, the sharp edge 329 and the angle of the front face 328 advantageously prevent this phenomenon. [0167] In the third exemplary CBA 3, some elements, such as the coupling support 306 have the same function as described in relation to the other CBAs, but the shape of such elements may be different. The benefits of some of the changes of the shapes are:

1) directing accidental fluid spills during refilling to follow a particular path for controlled draining and preventing them from reaching components such as the solenoid 214 and the load cell 203; and

2) some shapes are more suitable for injection moulding, which results in significant cost reduction compared to machining the parts. Some of the exemplary parts that can be manufactured by injection moulding include at least: the funnel 320 and the opening means 322; the bottle support 308, the coupling support 306; the fascia 100 and the fascia support 102; the locking bar 204; the lever support 211; the overload base 208, the electronic circuitry cover 222 and the base 400. The skilled person will appreciate that additional elements may be manufactured by injection moulding.

[0168] Figure 65 shows a method of opening and closing the funnel 320 of the bottle 300 of the CBAs 1, 2, 3 for refilling the reagent. This method illustrates how the components of the CBAs 1, 2, 3 of the present techniques interact with each other to achieve the opening and closing of the funnel 320 of the CBAs 1, 2, 3 for reagent refilling.

[0169] In step 1001, the method 1000 comprises scanning the reagent supply bottle for refilling. The reagent supply bottle has a scannable identification marker. The reagent supply bottle is used to refill the bottle 300 of the CBA 1, 2, 3. The scanning means may be positioned on the instrument (not shown). The scanning means may be also positioned on the CBA 1, 2, 3 (e.g. on the bottle 300 and/or the fascia 100) or in the vicinity of the CBA 1, 2, 3. In one example, an operative may scan the scannable identification marker of the reagent supply bottle with the instrument's scanning means. After or before scanning the reagent supply bottle, the operative may select the 'refill' operation with the instrument.

[0170] In step 1010, the method 1000 comprises initiating the fascia 100 unlock sequence. In other words, the instrument (not shown) selects an appropriate CBA for refill based on the scanned reagent supply bottle and sends a signal (ON power) to fascia locking solenoid 214 to unlock the selected CBA. The solenoid 214 is configured to receive an actuation signal from the instrument. The solenoid 214 is configured to process the actuation signal from the instrument. In an example, the reagent supply bottle has a 2D barcode, which when scanned allows the instrument to control a software to identify the correct CBA to open for that specific scanned reagent supply bottle. [0171] In step 1020, the method 1000 comprises moving the fascia 100 to an open fascia position ('second fascia position'). Initially, the fascia 100 may be in the closed position ('first fascia position'), wherein the fascia 100 is substantially close to the funnel 320 so that an operative is unable to access the funnel 320. In this step, after receiving the actuation signal from the instrument, the solenoid 214 actuates lever 212 which moves lever 212 in the downward direction, from a first lever position to a second lever position. By moving the lever 212 in the downward direction, the pin 210, which is positioned on the lever 212, is moved vertically down. Initially, the pin 210 may be caught in the first dowel pin 219. When the pin is engaged with the first dowel pin 219, the fascia 100 is in the closed position and the locking bar 204 cannot be moved. When the pin 210 is released from the first dowel pin 219, the locking bar 204 can move horizontally which allows the fascia 100 to move forward to an open position. By the movement of the locking bar 204, a compression spring (not shown) which is located under the fascia support 102 is actuated so that it can exert force on the fascia 100 to force the fascia 100 from the closed fascia position to an open fascia position. The skilled person will appreciate that the first lock pin depression and the second lock pin depression have the same functions as the first dowel pin 219 and the second dowel pin 220.

[0172] In step 1030, the method 1000 comprises sensing that the fascia 100 has been opened. In a first example, the movement of the locking bar 204 results in the movement of the first locking detection sensor 206. Therefore, the first locking detection sensor 206 and the second locking detection sensor 207 may sense that the fascia 100 has been opened. In a second example, the movement of the locking bar 204 actuates the mechanical switch 2060. Thus, the mechanical switch 2060 may sense the movement of the locking bar 204.

[0173] In step 1040, the method 1000 comprises detecting, by the instrument, the movement of the fascia. In one example, the sensor reading from the first and/or the second locking detection sensor 206, 207 which detect the movement of the fascia 100 are transmitted to the instrument. In other words, the first locking detection sensor 206 and/or the second locking detection sensor 207 send a signal to the instrument that the fascia 100 has been opened. In another example, the mechanical switch 2060 is configured to detect the movement of the fascia and transmit a result of this detection to the instrument.

[0174] In step 1050, the method 1000 comprises deactivating, by the instrument, the solenoid 214. In reply to the detection that the fascia 100 has moved, the instrument sends a second actuation signal (OFF power) to the solenoid 214 and deactivates the solenoid 214. In reply to the second actuation signal from the instrument, the solenoid 214 is configured to return to its original ('first') position and the movement of the fascia 100 stops at the open position ('refill' position). With the solenoid 214 returning to its original position, the lock pin 210 moves vertically back and engages the second dowel pin 220. Accordingly, the fascia 100 remains in the second fascia position (open position).

[0175] In step 1060, the method 1000 comprises opening the funnel 320. The funnel 320 is configured to be openable by an operative when the fascia 100 is in a 'refill' position (open fascia position/second fascia position). For instance, the operative may use only one finger to open the funnel 320 using an opening protrusion 322. The opening protrusion may be a hook. The hook may be shaped in such a way that it fits a human finger, therefore, the opening of the funnel 320 is easy. Beneficially, once the funnel 320 is open, the operative may pour the contents of a reagent refill bottle into the bottle 300, but the operative is unable to take the bottle 300 out of the CBA 1,2,3. Thus, the human error is reduced.

[0176] In step 1070, the method 1000 comprises closing the funnel 320. The skilled person will appreciate that the funnel 320 may be closed in more than one way. In the first option, in order to close the funnel 320, when the fascia 100 is in open ('refill') position, the operative may swing the funnel 320 to the closed position using only one finger. For instance, the operative may use the hook 322 to swing the funnel 320 back to its closed position. After that, the user may push the fascia 100 back to the closed position.

[0177] Alternatively, in order to close the funnel 320, when the fascia 100 is in open ('fill') position, the operative may push the fascia 100 to the closed position. In turn, the fascia 100 will push the funnel 320 to its closed position. Advantageously, this reduces human error and makes the CBA easier to operate.

[0178] Pushing the fascia 100 to the closed position, moves the locking bar 204 which forces the engagement of first dowel pin 219 with the lock pin 210. Once the first dowel pin 219 is engaged with the lock pin 210, the fascia 100 remains in the closed position (first fascia position).

[0179] In certain circumstances, it is desirable to remove the bottle 300 from the CBA (e.g. for service). In order to remove the bottle 300 from the CBA, the fascia 100 must be opened in a third fascia position ('bottle removal position'). In order to move the fascia 100 to the third position, an operative presses an appropriate button at the instrument screen to initiate the fascia 100 opening to the third position. This type of fascia 100 opening, follows the same opening sequence as for the refilling of the bottle 300, depicted in Figure 65, but the difference is that the solenoid 214 allows the fascia 100 to swing open until it reaches a hard stop. This is achieved by a screw fitted at the end of the locking bar 204. The head of the screw at the end of the locking bar 204 is above the locking bar 204 which prevents the locking bar 204 to travel further in the horizontal direction. Thus, by releasing the screw at the end of the locking bar, the fascia 100 travel limit to the third service position is achieved. Moreover, an appropriate time delay before releasing the solenoid 214 may be used in this sequence. After that, the bottle 300 may be entirely removed from the CBA. Then, the hard stop is removed from the end of the locking bar 204 which allows the fascia 100 to swing open to its maximum travel as shown in Figures 16, 33 and 53.

[0180] Figure 66 shows a schematic diagram of a system comprising an instrument and the CBA 1,2, 3 of the present techniques. The instrument 600 is configured to use a reagent. For instance, the instrument 600 is configured to mix several reagents. For instance, the instrument 600 may be a medical device. For instance, the instrument 600 may be used in relation to cancer treatment and/or diagnosis. In another example, the instrument 600 may be used in any other medical setting. The instrument 600 may be an automated staining instrument (e.g. Leica Biosystems Inc. BOND-PRIME Staining Instrument). The CBA 1, 2, 3 may be connected to the instrument 600. For instance, the CBA 1,2,3 may comprise a tube connector that connects the CBA 1, 2, 3 with the instrument 600 so that the reagent from the bottle 300 of the CBA 1, 2, 3 may be transferred to the instrument 600. The instrument 600 may comprise scanning means. The CBA 1, 2, 3 and the instrument 600 may be configured to be used in the method of Figure 65.

[0181] Reference throughout this specification to "one embodiment", "some embodiments" or "an embodiment" means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment of the present disclosure. Thus, appearances of the phrases "in one embodiment", "in some embodiments" or "in an embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures or characteristics may be combined in any suitable manner, as would be apparent to one of ordinary skill in the art from this disclosure, in one or more embodiments.

[0182] As used herein, unless otherwise specified the use of the ordinal adjectives "first", "second", "third", etc., to describe a common object, merely indicate that different instances of like objects are being referred to, and are not intended to imply that the objects so described must be in a given sequence, either temporally, spatially, in ranking, or in any other manner.

[0183] In the claims below and the description herein, any one of the terms comprising, comprised of or which comprises is an open term that means including at least the elements/features that follow, but not excluding others. Thus, the term comprising, when used in the claims, should not be interpreted as being limitative to the means or elements or steps listed thereafter. For example, the scope of the expression a device comprising A and B should not be limited to devices consisting only of elements A and B. Any one of the terms including or which includes or that includes as used herein is also an open term that also means including at least the elements/features that follow the term, but not excluding others. Thus, including is synonymous with and means comprising. In other words, where any or all of the terms "comprise", "comprises", "comprised" or "comprising" are used in this specification (including the claims) they are to be interpreted as specifying the presence of the stated features, integers, steps or components, but not precluding the presence of one or more other features, integers, steps or components.

[0184] It should be appreciated that in the above description of exemplary embodiments of the disclosure, various features of the disclosure are sometimes grouped together in a single embodiment, Fig., or description thereof for the purpose of streamlining the disclosure and aiding in the understanding of one or more of the various inventive aspects. This method of disclosure, however, is not to be interpreted as reflecting an intention that the claims require more features than are expressly recited in each claim. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single foregoing disclosed embodiment. Thus, the claims following the Detailed Description are hereby expressly incorporated into this Detailed Description, with each claim standing on its own as a separate embodiment of this disclosure.

[0185] Furthermore, while some embodiments described herein include some but not other features included in other embodiments, combinations of features of different embodiments are meant to be within the scope of the disclosure, and form different embodiments, as would be understood by those skilled in the art. For example, in the following claims, any of the claimed embodiments can be used in any combination.

[0186] In the description provided herein, numerous specific details are set forth. However, it is understood that embodiments of the disclosure may be practiced without these specific details. In other instances, well-known methods, structures and techniques have not been shown in detail in order not to obscure an understanding of this description.

[0187] Similarly, it is to be noticed that the term coupled, when used in the claims, should not be interpreted as being limited to direct connections only. The terms "coupled" and "connected", along with their derivatives, may be used. It should be understood that these terms are not intended as synonyms for each other. Thus, the scope of the expression a device A coupled to a device B should not be limited to devices or systems wherein an output of device A is directly connected to an input of device B. It means that there exists a path between an output of A and an input of B which may be a path including other devices or means. "Coupled" may mean that two or more elements are either in direct physical, electrical or optical contact, or that two or more elements are not in direct contact with each other but yet still co-operate or interact with each other.

[0188] Embodiments described herein are intended to cover any adaptations or variations of the present invention. Although the present invention has been described and explained in terms of particular exemplary embodiments, one skilled in the art will realize that additional embodiments can be readily envisioned that are within the scope of the present invention.

Claims

The claims defining the invention are as follows:

1. A closeable bulk fluid assembly (CBA) for an instrument and for holding a reagent and transferring the reagent to the instrument, comprising: a bottle for holding the reagent; the bottle comprising a funnel for filling the bottle, wherein the funnel is configured to move from a closed position to an open position; a fascia configured to move from a first fascia position to a second fascia position; wherein in the first fascia position, an operative is unable to access the funnel; and in the second fascia position, the operative is able to access the funnel and to move the funnel from the closed position to the open position to refill the bottle with the reagent and the operative is unable to remove the bottle out of the CBA.

2. The CBA according to claim 1, wherein the funnel comprises a hook for the operative to move the funnel from the closed position to the open position with one finger of the operative.

3. The CBA according to any one of the preceding claims, wherein the bottle further comprises a tube connector, wherein the tube connector is configured to connect the bottle to the instrument.

4. The CBA according to any one of the preceding claims, wherein the bottle further comprises a coupling support and a bottle lock.

5. The CBA according to any one of the preceding claims, wherein the CBA further comprises a bottle support configured to support the bottle.

6. The CBA according to claim 5, wherein the bottle support comprises two sloped wings for stabilizing the bottle in the bottle support.

7. The CBA according to any one of claims 5 to 6, wherein the bottle support is directly mounted on a load cell.

8. The CBA according to any one of the preceding claims, wherein the fascia is further configured to move to a third fascia position, wherein in the third fascia position, the bottle is removable from the CBA by the operative.

9. The CBA according to any one of the preceding claims, wherein the fascia comprises a pair of reinforcement ribs.

10. The CBA according to any one of the preceding claims, wherein the fascia comprises a fascia support to support the fascia so that the fascia is not in direct contact with the bottle.

11. The CBA according to claim 10, wherein one end of the fascia support forms a hinge mechanism so that the fascia is moveable from the first fascia position to the second fascia position and to the third fascia position.

12. The CBA according to any one of the preceding claims, wherein the CBA further comprises a locking mechanism for governing the movement of the fascia.

13. The CBA according to claim 12, wherein the locking mechanism comprises a locking bar; the locking bar comprises a fascia adjustment cam; wherein the fascia adjustment cam is configured to align the fascia with the instrument.

14. The CBA according to claim 13, wherein the locking bar is configured to move in horizontal direction with the movement of the fascia.

15. The CBA according to any one of claims 13 to 14, wherein the locking mechanism comprises a lock pin, a lever and a solenoid; the locking bar further comprises a first lock pin depression and a second lock pin depression; wherein the lock pin is positioned on the lever; wherein the lever is configured to move upon the actuation of the solenoid which moves the lock pin in a vertical direction; and when the lock pin is engaged with the first lock pin depression, the fascia is in the first fascia position and when the lock pin is engaged with the second lock pin depression, the fascia is in the second fascia position.

16. The CBA according to any one of claims 13 to 15, wherein the bottle support comprises a protrusion; wherein the locking bar comprises an overload protection cam rail; wherein the overload protection cam rail is configured to intake the protrusion when the fascia moves from the first fascia position to form an overload protection.

17. The CBA according to any one of the preceding claims, further comprising: a bottle protection locking mechanism; wherein the bottle protection locking mechanism comprises a bottle protection protrusion and a protection pin.

18. The CBA according to claim 17, wherein the bottle further comprises a bottle protection recess to accommodate the protection pin; and the bottle support further comprises a safety projection through which the protection pin interacts with the bottle.

19. The CBA according to any one of the preceding claims, further comprising: a mechanical switch configured to sense movement of the fascia.

20. The CBA according to any one of the claims 12 to 14, further comprising a first locking detection sensor, wherein the first locking detection sensor is moveable; wherein the locking mechanism comprises a second locking detection sensor; wherein the second locking detection sensor is fixed; and preferably wherein the first locking detection sensor and the second locking detection sensor are parts of an electromechanical switch.

21. A system comprising: a closeable bulk assembly (CBA) according to any one of the preceding claims; and an instrument configured to use the reagent.

22. The system of claim 21, wherein the instrument comprises a scanning means for scanning an identification marker of a reagent supply bottle; and wherein the instrument is configured to actuate the solenoid of the CBA based on the scanned identification marker.

23. The system of any one of the claims 21 to 22, wherein the instrument is an automated staining instrument.