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WO2025153796A1 - Appareil approprié pour préparer un échantillon destiné à une analyse - Google Patents

Appareil approprié pour préparer un échantillon destiné à une analyse

Info

Publication number
WO2025153796A1
WO2025153796A1 PCT/GB2024/053162 GB2024053162W WO2025153796A1 WO 2025153796 A1 WO2025153796 A1 WO 2025153796A1 GB 2024053162 W GB2024053162 W GB 2024053162W WO 2025153796 A1 WO2025153796 A1 WO 2025153796A1
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
container
sample
filter
optionally
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/GB2024/053162
Other languages
English (en)
Inventor
Rosemary Louise Burnell
Ralph George Lamble
Iason IOANNIDIS
William Ka Wing HO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotangents Ltd
Original Assignee
Biotangents Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB2401017.5A external-priority patent/GB2637356A/en
Application filed by Biotangents Ltd filed Critical Biotangents Ltd
Publication of WO2025153796A1 publication Critical patent/WO2025153796A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0605—Valves, specific forms thereof check valves

Definitions

  • the system may further comprise a cap, wherein the cap is configured to occlude the entrance aperture in order to prevent ingress of contaminants or to retain the fluid within the apparatus
  • Apparatus comprising an absorber
  • the invention provides an apparatus, suitable for preparing a sample for analysis, the apparatus comprising a body defining a cavity with an entrance aperture and an egress aperture arranged in fluid communication with the entrance aperture; wherein the body comprises a movable member, and an absorber associated with the body.
  • a fluid absorber can make samples easier to handle by retaining the fluid within the container, even if the apparatus is, for example, inverted.
  • the absorber may therefore remove impurities and also simplify sample collection.
  • the absorber may perform the aforesaid functions singularly or together.
  • the egress aperture is adapted to interface with a fluid extractor, such that the fluid, once absorbed by the absorber, may be extracted for further processing or analysis directly from the apparatus.
  • the movable member may be displaceable within the cavity between an entrapping position and a releasing position.
  • the movable member may be placed in either the entrapping position or the releasing position by depressing the egress aperture. Depressing the egress aperture should be achievable by hand and without the need of extra tooling.
  • the invention provides an apparatus, suitable for preparing a sample for analysis, the apparatus comprising: a body having first and second arms, the first arm bearing a fluid collector the second arm bearing an absorber.
  • a fluid collector can make collecting samples easier, providing a mechanical filter where particulate matter in the fluid is unable to enter the collector, e.g. via a weir or suitably dimensioned aperture.
  • An absorber can be sized to absorb a predetermined volume of fluid, thereby simplifying collection of the correct volume of a fluid.
  • the apparatus may also be provided as the body having only the first arm bearing the fluid collector and absent the second arm.
  • the fluid collector may comprise a reagent, wherein the reagent optionally comprises a lysing agent, optionally a bacteriocin, further optionally wherein the bacteriocin is lysostaphin.
  • the reagent may be contained on a bead.
  • the reagent may be contained within a rupturable chamber, such that the reagent may only be released once a user ruptures the rupturable chamber at the desired time.
  • the absorber may be pretreated with a reagent, wherein the reagent optionally is a buffer.
  • the absorber may be sized to absorb a predetermined volume of fluid, and optionally or alternatively, the absorber is arranged to release an absorbed fluid.
  • An absorber can be sized to absorb a predetermined volume of fluid, thereby simplifying collection of the correct volume of a fluid without additional fluid measuring devices.
  • a kit of parts comprising the apparatus described above may be provided, and further comprising one or more of a container; a rupturer; a cap.
  • the kit may suitably comprise a rupturer, wherein the rupturer is sized to accept the fluid collector, and the rupturer arranged to rupture the rupturable container.
  • the rupturer may comprise a piercing element in order to rupture the rupturable container.
  • the invention provides a kit of parts for collecting, filtering, and preparing a sample for analysis, the kit comprising: a container; an apparatus as discussed in any of the aspects disclosed above; and a processing container.
  • the kit optionally further includes a heater and/or an analyser, optionally wherein the analyser comprises a further filter.
  • the container may comprise an aperture sized to allow collection of 20 – 100 ml of a fluid, optionally collection directly from a living creature or processing line, and further optionally wherein the container comprises an indicator marker.
  • the above apparatus is applicable to the preparation of clinical mastitis milk samples in the field.
  • Such samples can be very variable in consistency depending on the host immune response and the causative pathogen.
  • the physical characteristics of each sample and the consistency of the sample vary greatly. Filtration to remove lumps and debris from the milk samples after heating allows the sample fluid to then be analysed.
  • Some fluids can solidify on initial processing (e.g. milk samples can solidify following a high heat incubation treatment). Subsequent handling of such samples is difficult and often results in protocol failure.
  • a solution to this issue is to provide a fluid absorber (not shown) in the tube (108).
  • the fluid collector may comprise a reagent (306), similar to the reagents discussed previously, that is, anything that a user wishes to introduce to a fluid once collected by the absorber, and the fluid released from the absorber.
  • the reagent (306) may, for example, be immobilized on a bead.
  • the reagent (306) may be, for example, lysing agent, optionally a bacteriocin, further optionally the bacteriocin is lysostaphin, or provided as a lysostaphin bead.
  • the reagent may also be a fluid or other solid preparation.
  • the reagent may be entrapped within a rupturable chamber within the fluid collector (303).

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un appareil, un kit, un système, et un procédé d'utilisation d'un appareil approprié pour préparer un échantillon destiné à une analyse. L'appareil comprend un corps définissant une cavité avec une ouverture d'entrée, un joint agencé sur le corps pour, lors de l'utilisation, venir en prise avec un récipient et former un joint étanche aux liquides entre le récipient et le corps, une vanne agencée en communication fluidique avec un filtre et conçue pour empêcher le fluide contenu à l'intérieur du corps de passer à travers l'ouverture d'entrée ; et le filtre étant agencé en communication fluidique avec l'ouverture d'entrée.
PCT/GB2024/053162 2024-01-19 2024-12-19 Appareil approprié pour préparer un échantillon destiné à une analyse Pending WO2025153796A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GR20240100031 2024-01-19
GR20240100031 2024-01-19
GB2401017.5A GB2637356A (en) 2024-01-19 2024-01-26 Apparatus suitable for preparing a sample for analysis
GB2401017.5 2024-01-26

Publications (1)

Publication Number Publication Date
WO2025153796A1 true WO2025153796A1 (fr) 2025-07-24

Family

ID=93962409

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2024/053162 Pending WO2025153796A1 (fr) 2024-01-19 2024-12-19 Appareil approprié pour préparer un échantillon destiné à une analyse

Country Status (1)

Country Link
WO (1) WO2025153796A1 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3512940A (en) * 1968-12-30 1970-05-19 Justin J Shapiro Test tube filter device
US4510058A (en) * 1982-08-15 1985-04-09 Technion Research & Development Foundation, Ltd. Method for a new type of chromatography and device therefor
US20110233119A1 (en) * 2010-03-29 2011-09-29 Nelson Steven D Sports bottle device with filter isolated from filtered fluid
US8211384B2 (en) * 2008-03-21 2012-07-03 Scientific Plastic Products, Inc. Filter vial
US9677981B2 (en) * 2013-03-15 2017-06-13 3M Innovative Properties Company Sample concentrator and method of use
US11045036B2 (en) * 2009-03-04 2021-06-29 O2C Raleigh, Llc Beverage filtering system

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3512940A (en) * 1968-12-30 1970-05-19 Justin J Shapiro Test tube filter device
US4510058A (en) * 1982-08-15 1985-04-09 Technion Research & Development Foundation, Ltd. Method for a new type of chromatography and device therefor
US8211384B2 (en) * 2008-03-21 2012-07-03 Scientific Plastic Products, Inc. Filter vial
US11045036B2 (en) * 2009-03-04 2021-06-29 O2C Raleigh, Llc Beverage filtering system
US20110233119A1 (en) * 2010-03-29 2011-09-29 Nelson Steven D Sports bottle device with filter isolated from filtered fluid
US9677981B2 (en) * 2013-03-15 2017-06-13 3M Innovative Properties Company Sample concentrator and method of use

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