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WO2025029630A3 - Aptamer-capture agent-based molecular sensors and methods of use - Google Patents

Aptamer-capture agent-based molecular sensors and methods of use Download PDF

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Publication number
WO2025029630A3
WO2025029630A3 PCT/US2024/039754 US2024039754W WO2025029630A3 WO 2025029630 A3 WO2025029630 A3 WO 2025029630A3 US 2024039754 W US2024039754 W US 2024039754W WO 2025029630 A3 WO2025029630 A3 WO 2025029630A3
Authority
WO
WIPO (PCT)
Prior art keywords
aptamer
capture agent
methods
analyte
molecular sensors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/039754
Other languages
French (fr)
Other versions
WO2025029630A2 (en
Inventor
Hyongsok Tom SOH
Ian Andrew Paul THOMPSON
Nicolo MAGANZINI
Dehui KONG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Leland Stanford Junior University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of WO2025029630A2 publication Critical patent/WO2025029630A2/en
Publication of WO2025029630A3 publication Critical patent/WO2025029630A3/en
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/7051—T-cell receptor (TcR)-CD3 complex

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided are molecular sensors comprising a capture agent that binds to a first epitope of an analyte, and an aptamer that binds to a second epitope of the analyte. The aptamer is stably associated with the capture agent and produces a detectable signal upon cooperative binding of the capture agent and aptamer to the analyte. Also provided are substrates comprising a molecular sensor of the present disclosure immobilized on the surface thereof, as well as devices comprising the molecular sensors and substrates. Methods of using the molecular sensors, substrates and devices of the present disclosure are also provided. In some instances, the methods are for assessing a sample for an analyte, the methods comprising contacting a molecular sensor of the present disclosure with the sample under conditions suitable for cooperative binding of the capture agent and aptamer to the analyte, and assessing for a detectable signal produced by the aptamer.
PCT/US2024/039754 2023-07-28 2024-07-26 Aptamer-capture agent-based molecular sensors and methods of use Pending WO2025029630A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363529633P 2023-07-28 2023-07-28
US63/529,633 2023-07-28

Publications (2)

Publication Number Publication Date
WO2025029630A2 WO2025029630A2 (en) 2025-02-06
WO2025029630A3 true WO2025029630A3 (en) 2025-03-13

Family

ID=94395855

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/039754 Pending WO2025029630A2 (en) 2023-07-28 2024-07-26 Aptamer-capture agent-based molecular sensors and methods of use

Country Status (1)

Country Link
WO (1) WO2025029630A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6503452B1 (en) * 1996-11-29 2003-01-07 The Board Of Trustees Of The Leland Stanford Junior University Biosensor arrays and methods
US20190359937A1 (en) * 2016-11-17 2019-11-28 Innovative Targeting Solutions Inc. Chimeric receptors, biosensor host cells and methods/uses thereof
US10605810B2 (en) * 2013-12-30 2020-03-31 University-Industry Cooperation Group Of Kyung Hee University Pincers comprising antibody and aptamer conjugated via a linker which binds to the same target material and use thereof
US20210355496A1 (en) * 2018-10-24 2021-11-18 Chan Zuckerberg Biohub, Inc. Compositions and methods involving aptamer switch polynucleotides

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6503452B1 (en) * 1996-11-29 2003-01-07 The Board Of Trustees Of The Leland Stanford Junior University Biosensor arrays and methods
US10605810B2 (en) * 2013-12-30 2020-03-31 University-Industry Cooperation Group Of Kyung Hee University Pincers comprising antibody and aptamer conjugated via a linker which binds to the same target material and use thereof
US20190359937A1 (en) * 2016-11-17 2019-11-28 Innovative Targeting Solutions Inc. Chimeric receptors, biosensor host cells and methods/uses thereof
US20210355496A1 (en) * 2018-10-24 2021-11-18 Chan Zuckerberg Biohub, Inc. Compositions and methods involving aptamer switch polynucleotides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HAN: "Design Strategies for Aptamer-Based Biosensors", SENSORS, 4 May 2010 (2010-05-04), pages 4541 - 4557, XP055044381, DOI: 10.3390/s100504541 *
KONG DEHUI, THOMPSON IAN A. P., MAGANZINI NICOLO, EISENSTEIN MICHAEL, SOH HYONGSOK TOM: "Aptamer–Antibody Chimera Sensors for Sensitive, Rapid, and Reversible Molecular Detection in Complex Samples", ACS SENSORS, AMERICAN CHEMICAL SOCIETY, US, vol. 9, no. 3, 22 March 2024 (2024-03-22), US, pages 1168 - 1177, XP093291557, ISSN: 2379-3694, DOI: 10.1021/acssensors.3c01638 *

Also Published As

Publication number Publication date
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