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WO2025028931A1 - Procédé d'amélioration de la teneur en ginsénosides du panax ginseng contenant un solvant colloïdal d'argent, et composition de panax ginseng ayant une teneur accrue en ginsénosides - Google Patents

Procédé d'amélioration de la teneur en ginsénosides du panax ginseng contenant un solvant colloïdal d'argent, et composition de panax ginseng ayant une teneur accrue en ginsénosides Download PDF

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Publication number
WO2025028931A1
WO2025028931A1 PCT/KR2024/010911 KR2024010911W WO2025028931A1 WO 2025028931 A1 WO2025028931 A1 WO 2025028931A1 KR 2024010911 W KR2024010911 W KR 2024010911W WO 2025028931 A1 WO2025028931 A1 WO 2025028931A1
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Prior art keywords
ginseng
ppm
silver
ginsenoside content
improving
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English (en)
Korean (ko)
Inventor
피재호
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Ingr Inc
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Ingr Inc
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/35—Bulbs; Alliums, e.g. onions or leeks
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • C05G5/27—Dispersions, e.g. suspensions or emulsions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/15—Inorganic Compounds
    • A23V2250/156—Mineral combination
    • A23V2250/1562—Silver
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00—Food ingredients
    • A23V2250/20—Natural extracts
    • A23V2250/21—Plant extracts
    • A23V2250/2124—Ginseng

Definitions

  • the present invention relates to a method for improving the ginsenoside content of ginseng including a silver colloidal solvent and a ginseng composition having increased ginsenoside content.
  • Ginseng ( Panax ginseng ) is a perennial shade-loving herbaceous plant belonging to the genus Panax in the family Araliaceae, and has long been used as an important medicinal ingredient in oriental medicine.
  • Ginseng is generally classified into white ginseng and red ginseng depending on the processing method.
  • White ginseng is unprocessed ginseng dug from the field, that is, fresh ginseng that has been dried as is, while red ginseng is fresh ginseng that has been steamed, dried, and processed.
  • various chemical changes such as saponin modification, amino acid change, and browning occur. Since ginseng contains saponin, excellent pharmacological effects such as cancer prevention, cancer cell growth inhibition, blood pressure lowering, brain nerve cell protection, and learning ability improvement can be expected.
  • Ginseng saponin is called ginsenoside, which is a name given to the glycoside separated from ginseng in order to distinguish it from other plant saponins. It is usually named ginsenoside-Rx, etc. (abbreviated as G-Rx). Here, “R” means Radix or Root. In addition, “x” is named in order from bottom to top according to the migration distance (Rf value) of the spot that appears on the TLC, such as o, a, b, c, d, e, f, g, h.
  • the types of ginsenosides currently known to be contained in ginseng include Rg1, Rb1, Rg2, Rc, and Rd.
  • Korean Patent No. 10-0464901 discloses a bactericidal trace element fertilizer containing silver colloid particles
  • Korean Patent Publication No. 2016-0086149 discloses a composition containing a ginseng extract with increased ginsenoside content as an active ingredient
  • Korean Patent No. 10-1603169 discloses a method for producing a ginseng extract with increased acidic polysaccharide and ginsenoside content.
  • no method of using a silver colloid solvent to increase the content of ginsenoside according to the present invention has been disclosed.
  • Ag-NP silver nano-particle
  • the present invention aims to provide a method of utilizing a silver colloidal solvent to improve the content of ginseng ginseng by analyzing the effect of applying a silver colloidal solvent having a different composition to ginseng on the growth and differentiation of ginseng and analyzing the change in the content of ginsenoside in ginseng roots.
  • Patent Document 0001 Republic of Korea Registered Patent No. 10-0464901
  • Patent Document 0002 Republic of Korea Publication Patent No. 2016-0086149
  • Patent Document 0003 Republic of Korea Registered Patent No. 10-1603169
  • the present invention provides a method for improving the ginsenoside content of ginseng including a silver colloidal solvent and a ginseng composition with increased ginsenoside content.
  • the present invention provides a method for improving the ginsenoside content of ginseng comprising a silver colloidal solvent.
  • the present invention provides a ginseng composition having increased ginsenoside content by the above method.
  • the ginsenoside content can be increased with only two fertilizations at a low concentration of 10 ppm at the harvest time without having to fertilize consistently for six years.
  • Figure 2 shows the results of ginseng growth-differentiation analysis according to one embodiment of the present invention (Experimental Example 1), showing the results of measuring the length of each ginseng stem when the silver colloid solvents of Examples 1 to 5 were applied.
  • Figure 8 shows the results of ginseng ginsenoside analysis according to one embodiment of the present invention (Experimental Example 2), showing 10 major ginsenosides in the V1 treatment group and unknown substances A to N that increased compared to the control group.
  • Figure 9 shows the results of ginseng ginsenoside analysis according to one embodiment of the present invention (Experimental Example 2), showing 10 major ginsenosides in the En treatment group and unknown substances A to N that increased compared to the control group.
  • Figure 11 shows the results of analyzing 10 types of ginsenosides in ginseng roots treated with silver colloid solvents according to Examples 1 to 3 of the present invention.
  • Figure 12 shows the results of comparative analysis of protopanaxatriol (PPT) series ginsenosides in ginseng roots treated with silver colloid solvents of Examples 1 to 3 of the present invention.
  • PPT protopanaxatriol
  • Figure 13 shows the results of comparative analysis of protopanaxadiol (PPD) series ginsenosides in ginseng roots treated with silver colloid solvents of Examples 1 to 3 of the present invention.
  • PPD protopanaxadiol
  • Figure 14 shows the total contents of 10 kinds of ginsenosides in ginseng roots treated with silver colloid solvents of Examples 1 to 3 of the present invention.
  • One embodiment of the present invention provides a method for improving the ginsenoside content of ginseng, comprising a silver colloidal solvent.
  • the above silver colloidal solvent may be composed solely of silver colloid, but is not limited thereto.
  • the above silver colloid concentration may be 500 to 5,000 ppm, specifically 1,000 to 4,500 ppm, 1,500 to 4,500 ppm, 1,500 to 4,000 ppm, 2,000 to 4,000 ppm, more specifically 2,100 to 3,900 ppm, 2,200 to 3,800 ppm, 2,300 to 3,700 ppm, 2,400 to 3,600 ppm, 2,500 to 3,500 ppm, 2,600 to 3,400 ppm, 2,700 to 3,300 ppm, 2,800 to 3,200 ppm, 2,900 to 3,100 ppm, 3000 ppm. However, it is not limited to these.
  • the silver colloid solvent of the present invention may include silver colloid and a main component of a fertilizer.
  • the main component of the fertilizer may be N, P, K, but is not limited thereto. Specifically, it may be at least one selected from the group consisting of nitrogen, phosphoric acid, potassium, manganese, boron, molybdenum, zinc, and copper.
  • the component may be water-soluble, but is not limited thereto.
  • the nitrogen may be in an amount of 1 to 10 wt% based on the total solvent weight. Specifically, 2 to 10 wt%, 3 to 10 wt%, 4 to 10 wt%, 5 to 10 wt%, 5 to 9 wt%, 5 to 8 wt%, more specifically, 5.5 to 8 wt%, 6 to 8 wt%, 6.5 to 8 wt%, 7 to 8 wt%, even more specifically, 7.1 to 7.9 wt%, 7.2 to 7.8 wt%, 7.3 to 7.7 wt%, 7.4 to 7.6 wt%, 7.5 wt%, but is not limited thereto.
  • the phosphoric acid may be present in an amount of 0.5 to 5 wt% based on the total solvent weight. Specifically, 0.5 to 4.5 wt%, 0.5 to 4 wt%, 0.5 to 3.5 wt%, 0.5 to 3 wt%, 0.5 to 2.5 wt%, 0.5 to 2 wt%, more specifically, 0.6 to 1.9 wt%, 0.7 to 1.8 wt%, 0.8 to 1.7 wt%, 0.9 to 1.6 wt%, 1 to 1.5 wt%, 1.1 to 1.4 wt%, 1.1 to 1.3 wt%, 1.2 wt%, but is not limited thereto.
  • the above potassium permanganate may be present in an amount of 1 to 5 wt% based on the total solvent weight. Specifically, the potassium permanganate may be present in an amount of 1 to 4.5 wt%, 1 to 4 wt%, 1 to 3.5 wt%, 1 to 3 wt%, 1.5 to 3 wt%, more specifically, 1.6 to 3 wt%, 1.7 to 3 wt%, 1.8 to 3 wt%, 1.9 to 3 wt%, 2 to 3 wt%, 2.1 to 2.9 wt%, 2.2 to 2.8 wt%, 2.3 to 2.6 wt%, 2.5 wt%, but is not limited thereto.
  • the manganese may be present in an amount of 0.01 to 1 wt% based on the total solvent weight. Specifically, 0.01 to 0.9 wt%, 0.01 to 0.8 wt%, 0.01 to 0.7 wt%, 0.01 to 0.6 wt%, 0.01 to 0.5 wt%, 0.01 to 0.4 wt%, 0.01 to 0.3 wt%, 0.01 to 0.2 wt%, more specifically, 0.02 to 0.2 wt%, 0.03 to 0.2 wt%, 0.04 to 0.2 wt%, 0.05 to 0.2 wt%, 0.06 to 0.2 wt%, 0.07 to 0.2 wt%, 0.08 to 0.2 wt%, 0.09 to 0.2 wt%, 0.1 to 0.2 wt%, 0.11 to 0.19 wt%, It may be 0.12 to 0.18 wt%, 0.13 to 0.17 wt%, 0.14 to 0.16 wt%, or 0.15 wt%,
  • the boron may be present in an amount of 0.001 to 1 wt% based on the total solvent weight. Specifically, the boron may be present in an amount of 0.001 to 0.5 wt%, 0.005 to 0.5 wt%, 0.01 to 0.5 wt%, 0.01 to 0.4 wt%, 0.01 to 0.3 wt%, 0.01 to 0.2 wt%, 0.01 to 0.1 wt%, more specifically, 0.02 to 0.1 wt%, 0.03 to 0.1 wt%, 0.04 to 0.1 wt%, 0.05 to 0.1 wt%, 0.06 to 0.1 wt%, 0.07 to 0.1 wt%, 0.07 to 0.09 wt%, or 0.08 wt%, but is not limited thereto.
  • the above molybdenum may be 0.001 to 1 wt% based on the total solvent weight. Specifically, 0.001 to 0.5 wt%, 0.001 to 0.4 wt%, 0.001 to 0.3 wt%, 0.001 to 0.2 wt%, 0.001 to 0.1 wt%, more specifically, 0.001 to 0.09 wt%, 0.002 to 0.09 wt%, 0.003 to 0.09 wt%, 0.004 to 0.09 wt%, 0.005 to 0.09 wt%, 0.006 to 0.09 wt%, 0.007 to 0.09 wt%, 0.008 to 0.09 wt%, 0.009 to 0.09 wt%, 0.01 to 0.09 wt%, and even more specifically, 0.01 to 0.08 wt%, It may be 0.01 to 0.07 wt%, 0.01 to 0.06 wt%, 0.01 to 0.05 wt%, 0.01 to 0.04 wt%,
  • the above zinc may be 0.01 to 1 wt% based on the total solvent weight. Specifically, it may be 0.01 to 0.9 wt%, 0.01 to 0.8 wt%, 0.01 to 0.7 wt%, 0.01 to 0.6 wt%, 0.01 to 0.5 wt%, 0.02 to 0.5 wt%, 0.03 to 0.5 wt%, 0.04 to 0.5 wt%, 0.05 to 0.5 wt%, 0.06 to 0.5 wt%, 0.07 to 0.5 wt%, 0.08 to 0.5 wt%, 0.09 to 0.5 wt%, 0.1 to 0.5 wt%, more specifically, 0.1 to 0.4 wt%, 0.1 to 0.3 wt%, 0.15 to 0.25 wt%, 0.2 wt%, but is not limited thereto.
  • the above copper may be 0.01 to 1 wt% based on the total solvent weight. Specifically, 0.01 to 0.9 wt%, 0.01 to 0.8 wt%, 0.01 to 0.7 wt%, 0.01 to 0.6 wt%, 0.01 to 0.5 wt%, 0.01 to 0.4 wt%, 0.01 to 0.3 wt%, 0.01 to 0.2 wt%, more specifically, 0.01 to 0.19 wt%, 0.02 to 0.18 wt%, 0.03 to 0.17 wt%, 0.04 to 0.16 wt%, 0.05 to 0.15 wt%, 0.06 to 0.14 wt%, 0.07 to 0.13 wt%, 0.08 to 0.12 wt%, 0.09 to 0.11 wt%, 0.1 wt%. However, it is not limited to this.
  • the silver colloid solvent of the present invention may include a silver colloid and a silver particle stabilizer.
  • the silver particle stabilizer may be at least one selected from the group consisting of carbonic acid, anionic phosphorus-containing surfactants, anionic polymers, and nitrogen-containing polymers, but is not limited thereto.
  • the concentration of the silver particle stabilizer may be 1,000 to 50,000 ppm. Specifically, 1,000 to 40,000 ppm, 1,000 to 30,000 ppm, 1,000 to 20,000 ppm, 2,000 to 20,000 ppm, 3,000 to 20,000 ppm, 4,000 to 20,000 ppm, 5,000 to 20,000 ppm, 6,000 to 20,000 ppm, 7,000 to 20,000 ppm, 8,000 to 20,000 ppm, 9,000 to 20,000 ppm, 10,000 to 20,000 ppm, more specifically, 11,000 to 20,000 ppm, 12,000 to 20,000 ppm, 13,000 to 20,000 ppm, 14,000 to 20,000 ppm, 15,000 to 20,000 ppm, 15,000 to 19,000 ppm, 15,000 to 18,000 ppm, 15,000 to 17,000 ppm, 15,500 to 16,500 ppm, 16,000 ppm, but is not limited thereto.
  • one embodiment of the present invention provides a ginseng composition having increased ginsenoside content by the above method.
  • a silver colloidal solvent containing silver colloid and anionic phosphorus-containing surfactant as a silver particle stabilizer was prepared and named Variant 2 (Eunjandi 2).
  • a silver colloidal solvent containing silver colloid and anionic high molecular, nitrogen-containing polymer as a silver particle stabilizer was prepared and named Variant 3 (Eunjandi 3).
  • Example 1 Zeroxxe 3,000 -
  • Example 2 Eunbi 2,200 Nitrogen 7.5%, water-soluble phosphoric acid 1.2%, water-soluble potassium 2.5%, water-soluble manganese 0.15%, water-soluble boron 0.08%, water-soluble molybdenum 0.01%, water-soluble zinc 0.2%, water-soluble copper 0.1%,
  • Figures 1 to 7 show the results of comparative analysis of growth information of 6-year-old phosphoric acid according to treatment of silver colloid products manufactured in Examples 1 to 5.
  • Ginseng root samples treated with liquid nitrogen were finely ground and extracted and purified by adding 10 ml of 50% MeOH per 1 g.
  • the samples were analyzed using a UV detector (203 nm) under gradient conditions at a flow rate of 1 ml/min using high-performance liquid chromatography (HPLC) Agilent 110 series, Waters Symmetry C18 column.
  • HPLC high-performance liquid chromatography
  • Figure 8 shows the 10 major ginsenosides in the V1 treatment group and the unknown substances A to N that increased compared to the control group
  • Figure 9 shows the 10 major ginsenosides in the En treatment group and the unknown substances A to N that increased compared to the control group
  • Figure 10 shows the 10 major ginsenosides in the Zx treatment group and the unknown substances A to N that increased compared to the control group.
  • the substance corresponding to D was identified only in the Zx treatment and not in En or V1, and all substances A to N except D were found to be enhanced in the silver colloid treatment group.
  • Figure 11 shows the results of analyzing 10 types of ginsenosides in ginseng roots treated with silver colloid in Examples 1 to 3
  • Figure 12 shows the results of comparing and analyzing protopanaxatriol (PPT)-series ginsenosides
  • Figure 13 shows the results of comparing and analyzing protopanaxadiol (PPD)-series ginsenosides.
  • Rg1, Rf, and Rc were significantly increased (P ⁇ 0.001), and Rb1 and Rh1 were increased in content at the P ⁇ 0.01 level.
  • Re and Rg2 were confirmed to have decreased in content at a very significant level (P ⁇ 0.001).
  • Rg1, Rf, Rb1, Rg2, Rh1, Rc, Rb2, and Rd were significantly increased (P ⁇ 0.001), and Rb3 content increased at the level of P ⁇ 0.01. Re content was confirmed to decrease at the level of P ⁇ 0.01.

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Abstract

La présente invention concerne : un procédé d'amélioration de la teneur en ginsénosides de Panax ginseng contenant un solvant colloïdal d'argent ; et une composition de Panax ginseng ayant une teneur accrue en ginsénosides. L'utilisation du procédé de la présente invention a pour effet de permettre d'augmenter la teneur en ginsénosides par apport d'engrais juste deux fois à une faible concentration de 10 ppm au moment de la récolte sans avoir à apporter de l'engrais de manière constante pendant 6 ans.
PCT/KR2024/010911 2023-07-28 2024-07-26 Procédé d'amélioration de la teneur en ginsénosides du panax ginseng contenant un solvant colloïdal d'argent, et composition de panax ginseng ayant une teneur accrue en ginsénosides Pending WO2025028931A1 (fr)

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KR10-2023-0098995 2023-07-28

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KR101603169B1 (ko) 2015-09-17 2016-03-14 대동고려삼 주식회사 산성다당체 및 진세노사이드 함량이 증진된 인삼 추출물의 제조방법

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