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WO2025024701A3 - 3d spatial single cell omics by implantation barcoding - Google Patents

3d spatial single cell omics by implantation barcoding Download PDF

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Publication number
WO2025024701A3
WO2025024701A3 PCT/US2024/039614 US2024039614W WO2025024701A3 WO 2025024701 A3 WO2025024701 A3 WO 2025024701A3 US 2024039614 W US2024039614 W US 2024039614W WO 2025024701 A3 WO2025024701 A3 WO 2025024701A3
Authority
WO
WIPO (PCT)
Prior art keywords
single cell
spatial
implantation
barcoding
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/039614
Other languages
French (fr)
Other versions
WO2025024701A2 (en
Inventor
Wing H. Wong
Tungyu WU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Leland Stanford Junior University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of WO2025024701A2 publication Critical patent/WO2025024701A2/en
Publication of WO2025024701A3 publication Critical patent/WO2025024701A3/en
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Described herein are methods, compositions, devices, and kits related to methods for 3-dimensional (3D) spatial single cell omics analysis based on the concept of spatial encoding by positional markers injected with implantation devices (for example, jetlet dispensors). Aspects of the present disclosure provide for at least the following: 1) single cell multiomics: a multiomic profile is generated for each single cell in the sample; and 2) 3D mapping: the single cells can be mapped to 3D spatial locations at cellular resolution.
PCT/US2024/039614 2023-07-26 2024-07-25 3d spatial single cell omics by implantation barcoding Pending WO2025024701A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363529017P 2023-07-26 2023-07-26
US63/529,017 2023-07-26

Publications (2)

Publication Number Publication Date
WO2025024701A2 WO2025024701A2 (en) 2025-01-30
WO2025024701A3 true WO2025024701A3 (en) 2025-04-24

Family

ID=94375491

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/039614 Pending WO2025024701A2 (en) 2023-07-26 2024-07-25 3d spatial single cell omics by implantation barcoding

Country Status (1)

Country Link
WO (1) WO2025024701A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170337459A1 (en) * 2015-02-27 2017-11-23 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2020086510A1 (en) * 2018-10-22 2020-04-30 The General Hospital Corporation Multiplexed single-cell analysis using optically-encoded rna capture particles

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170337459A1 (en) * 2015-02-27 2017-11-23 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2020086510A1 (en) * 2018-10-22 2020-04-30 The General Hospital Corporation Multiplexed single-cell analysis using optically-encoded rna capture particles

Also Published As

Publication number Publication date
WO2025024701A2 (en) 2025-01-30

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