WO2025019917A1 - Method for acting on elements present in the soil, water or air by means of sequences of extremely low frequencies - Google Patents

Abstract

The present invention relates to a method for using sequences of extremely low frequencies that makes it possible to act on elements in the soil, water and air by means of defining targets and specific sets of guides for this type of actuation. The desired effect is achieved by lengthening or shortening the bonds between the atoms that form chemical elements and their combinations, including the constituent elements of plant-related nutrients and gases.

Description

PROCESS FOR ACTING ON ELEMENTS PRESENT IN THE SOIL, WATER OR AIR BY MEANS OF EXTREMELY LOW FREQUENCY SEQUENCES

DESCRIPTIVE REPORT

FIELD OF INVENTION

[0001] This invention deals with a process for using extremely low frequency sequences that allows action on elements on the ground, in water and in the air by defining targets and sets of drivers specific to this type of action.

[0002] The desired effect is achieved by distancing or bringing together the bonds between the atoms that form chemical elements and their combinations, including the constituent elements of nutrients and gases related to plants.

PREVIOUS

[0003] In general, the use of various mineral elements from different sources (organic or inorganic) is common in agriculture today. We know that the choice of elements and their sources directly impacts crop productivity and that the costs related to the use of these molecules are relevant when considering the economic analysis of agricultural activity. There are currently two most common types of solutions available to stimulate plant growth: mineral and organic fertilizers. Both have been used, including together, but they can have undesirable effects such as contamination of soil, rivers, lakes and groundwater, poisoning of professionals who apply the products to crops, among others.

[0004] The invention filed on 10/30/2018, under number BR102017023442-8 and title "Product and Process for Generating Extremely Low Frequencies for "Chemical Equalization" describes a process and product for chemical equalization, based on the transmission of frequencies that will act on the equalization of chemical elements or a substance, consequently providing the adjustment of strengthening or attenuation of their physical-chemical properties and characteristics as desired, improving quality, increasing productivity and efficiency of industrial processes.

[0005] By strengthening the physical-chemical properties and characteristics of an element, it is possible to use it in significantly lower doses, without, however, impacting the results obtained. This invention, however, does not have the structure of directors, capable of guiding the frequency sequences to specific targets, nor the ability to act on the ground and air.

[0006] The invention BR 10 2019 025958 2 - PRODUCT AND PROCESS FOR ACTING ON BIOLOGICAL ORGANISMS BY MEANS OF EXTREMELY LOW FREQUENCIES of 12/07/2019 reveals a product and process for using extremely low frequency sequences for use in organisms. However, said invention does not define specific processes for the use of this procedure in the case of plants.

[0007] The publication Exposure to Sound Vibrations Lead to Transcriptomic, Proteomic and Hormonal Changes in Arabidopsis of 26/09/2016 - Ghosh, R., Mishra, R., Choi, B. et al. Exposure to Sound Vibrations Lead to Transcriptomic, Proteomic and Hormonal Changes in Arabidopsis. Sci Rep 6, 33370 (2016). https://doi.org/10.1038/srep33370 - reveals that sound vibration (SV) is considered as an external mechanical force that modulates plant growth and development as other mechanical stimuli (e.g., wind, rain, touch, and vibration). A number of previous and recent studies have reported developmental responses in adapted plants against SV of varying frequencies. This strongly suggests the existence of sophisticated molecular mechanisms for SV perception and signal transduction. Despite this, there is a huge gap in our understanding of the changes SV-mediated molecular changes, which is a prerequisite for gaining insight into SV-mediated plant development. Here, we investigated the global gene expression changes in Arabidopsis thaliana after treatment with five different single frequencies of SV at constant amplitude for 1 h. As a next step, we also studied the SV-mediated proteomic changes in Arabidopsis. The data suggested that, like other stimuli, SV also activated signature cellular events, e.g., scavenging of reactive oxygen species (ROS), alteration of primary metabolism, and hormonal signaling. Phytohormonal analysis indicated that VS-mediated responses were, in part, modulated by specific changes in phytohormones levels; especially salicylic acid (SA). Notably, several touch-regulated genes were also regulated by SV treatment suggesting a possible molecular crosstalk between the two mechanical stimuli, sound and touch.

[0008] However, the aforementioned publication does not reveal the use of specific frequency sequences for the observed effect, reporting only changes resulting from constant frequency stimulation.

[0009] The publication SOUND WAVE IN PLANT GROWTH REGULATION: A REVIEW OF POTENTIAL BIOTECHNOLOGICAL APPLICATIONS - Mohanta, Tapan Kumar. Sound wave in plant growth regulation: a review of potential biotechnological applications. JAPS: Journal of Animal & Plant Sciences, v. 28, n. 2, 2018. - reveals that the perception of sound waves by animals is one of the most important means of communication. It has contributed significantly to the formation of the behavior, ecology and evolution of the organism. This evolutionary conservation mechanism of communication in animals is carried out by different specialized organs such as ear, antenna and others. However, the sessile organism, plants do not have the specialized organ for the perception of sound and still perceive the sound signal. The perception of sound waves in plants can also play a critical role in the ecological and evolutionary formation. The effect of sound waves on the plant can have an effect advantageous that allows it to learn about the surrounding environment using acoustic energy. The perceptions of sound signal in plants can lead to increased growth, development and productive potential of plants. Therefore, sound wave treatment in plant can be used as a growth promoter/regulator to increase the yield potential of crops. However, the receptor molecule that perceives sound wave in plants has not yet been identified. The mechano-sensitive ion channels present in the plasma membrane are highly modulated due to sound wave treatment which leads to differential calcium signaling in plants and subsequent regulation of downstream signaling molecules. In this review, the author reported some basic concepts of sound wave, research and modulation of molecular responses in plants at cellular and subcellular level after sound wave treatment.

[0010] However, the aforementioned publication does not reveal the use of specific frequency sequences for the observed effect, reporting only changes resulting from constant frequency stimulation.

[0011] The publication Sound waves delay tomato fruit ripening by negatively regulating ethylene biosynthesis and signaling genes https://doi.org/10.1016/j-postharvbio.2015.07.015 reveals that tomato fruits were investigated whether appropriate treatment could delay their ripening. Harvested fruits were treated with low-frequency sound waves (1 kHz) for 6 h and then several fruit characteristics were monitored for 14 to 23 days. Seven days after treatment, 85% of the treated fruits were green, versus less than 50% of the untreated fruit. Most of the tomato fruit turned red ripening by 14 days after treatment. Ethylene production and respiration rate were lower in acoustically treated than untreated tomatoes. Furthermore, changes in surface color and flesh firmness were delayed in the treated fruit. To investigate how sound wave treatment affects fruit ripening, the expression of ethylene-related genes was analyzed by real-time quantitative RT-PCR analysis. real. The expression level of several ethylene biosynthetic (ACS2, ACS4, AC01, E4, and E8) and ripening-regulated (RIN, TAGL1, HB-1, NOR, CNR) genes was influenced by sound wave treatment. These results indicated that sound wave treatment delays tomato fruit ripening by altering the expression of genes important in ethylene biosynthesis and ethylene signaling pathways.

[0012] However, the aforementioned publication does not reveal the use of specific frequency sequences for the observed effect, reporting only changes resulting from constant frequency stimulation.

GENERAL DESCRIPTION

[0013] Since the controlled use of frequency sequences is something new, it is natural for there to be gaps in the state of the art, as demonstrated. To resolve the usage demands, we then developed a new technique revealed below.

[0014] The technique developed here makes use of an existing transmitter of frequency sequences calculated according to the elements on which it is desired to act, determining a process that obtains the desired effects from the point of view of acting on the ground, water or air, being able to act on more than one of these objects at the same time and on more than one element in these objects also at the same time.

[0015] In practice, one must start from the desired effect, as specified by demand, to determine targets and drivers that are passed to the existing system for determining the frequency sequences that will act on the targets when in conjunction with the drivers and will produce the desired effect.

[0016] Thus the process revealed here consists of:

[0017] Phase 1: determining the plants' needs. This phase begins with the input of data collected from the land where the planting will occur (or occurs) and Depending on the plant to be planted, we calculate whether the plant's needs can be met by the soil or whether it is necessary to add missing elements. These determinations may consider the soil's extra nutritional capacity made possible by the use of technologies based on frequency sequences. In practice, the data from this phase may be provided by third parties, such as the planters themselves. In this case, the data on the existence of the necessary nutrients produced by third parties, which may be the producer himself and his soil correction processes, are accepted and we immediately proceed to phase 2 of the process.

[0018] Phase 2: Determination of targets and drivers (for each element considered important in plant nutrition). Elements that should be more bioavailable and more easily metabolized are identified and a target and driver are defined for each one. The target is the element that should be more or less absorbed and more or less easily metabolized by the plant. The driver is defined so that its combination with the target produces a unique combination, that is, the frequency sequence that will be directed to the target will only act on the target when it is combined with the driver and this will only occur in the circumstances in which it is desired to act on the target. In parallel, the following are also defined:

[0019] a) The objective of the action: to distance or bring the atoms of the target elements closer together according to the objective of making the target more or less bioavailable and more or less easily metabolizable by the plant.

[0020] b) The object of the action: the need for the action to take place in the natural soil or in fertilizers (to be added), in the water or in the air that permeates the plantation. [0021] Phase 3: Communication with the frequency sequence generator: The sets of targets and drivers and the objective of the action (which automatically already presupposes the object) are sent to the frequency sequence generator, which returns with a set of frequency sequences that will be sent to the plantation. [0022] Phase 4: Sending to the application device: The sets of frequency sequences are sent to the application device which can be RFID cards, satellites or other means of emitting the frequency sequences.

[0023] Phase 5: Database and Intelligence: The sets of targets, drivers and objectives are stored for future use when in similar circumstances.

[0024] The action can take place (object of the action):

[0025] 1. A set of atoms (chemical molecule) naturally present in the soil and/or generated from the degradation, metabolism, chemical reactions or transformation of fertilizer in the soil. In some cases, the chemical molecule, when applied to the soil, undergoes changes due to reaction with other molecules or through a process of metabolism by microorganisms present in the soil. After this transformation, the molecule that will be absorbed by the plants is generated (the form of the nutrient that can be absorbed by the plant). In this case, the aim is to strengthen or attenuate the bonds between the chemical elements to facilitate or hinder the process of metabolizing this form that can be absorbed by the plant.

[0026] 2. About a set of atoms (chemical molecule) used as fertilizer for application to the soil or directly to the plant. In this case, the aim is to strengthen or attenuate the bonds between the chemical elements to facilitate or hinder the process of metabolization (degradation) of these nutrients in the soil and/or by the plant.

[0027] 3. About a set of atoms (chemical molecule) present in the air (atmosphere) that are absorbed by the plant. In this case, the aim is to strengthen or attenuate the bonds between the chemical elements to facilitate or hinder the process of metabolization (degradation) of these compounds during their use in the physiological processes carried out by the plant.

[0028] The process described above is more easily implemented by means of a computer program with the appropriate interfaces for data input and output, processing modules, communication modules/interfaces, analysis and simulation of the joint effect of actions on the ground, among others. [0029] List of Figures

[0030] Figure 1: illustrates the process phases

[0031] Figure 2: Representation of the carbon dioxide (CO2) molecule in the air that will be absorbed by the plant for the photosynthesis process. A) CO2 molecule represented in its natural form; B) CO2 molecule represented after the action of the frequency sequences, showing the distance between the atoms.

[0032] Figure 3: Representation of the ammonium molecule (NH4) in the soil that will be absorbed by the plant as a source of nitrogen. A) NH4 molecule represented in its natural form; B) NH4 molecule represented after the action of the frequency sequences, showing the distance between the atoms.

[0033] Figure 4: Representation of the amino acid sequence that forms the HRP Enzyme and some site annotations found on the UNIPROT website (https://www.uniprot.org/uniprotkb/K7ZW59/entry). Note that the highlighted amino acid sequence was used as a target for calculating the frequency sequences to be used in the test.

[0034] Figure 5: Image of the test development plate after 10 minutes of enzymatic action. Note the visual difference in color between the control and treated (test) samples.

[0035] Figure 6: Vases labeled with a microchip that has the function of emitting extremely low frequency sequences into the soil. In detail, microchip emitting extremely low frequencies that are glued directly to the vases.

[0036] Next we will reveal some implementations of the process revealed above.

PREFERRED MODALITIES

[0037] The first preferred embodiment targets the urea molecule [CO(NH2)2] which is used as a nitrogen source for plants. In this case the sequences of frequencies will act on the set of atoms that form the chemical molecule in question. As a consequence of this action, the chemical bond between the atoms will be strengthened or weakened, causing a change in the energy requirement for the metabolization process of this molecule, that is, facilitating or hindering its transformation in the soil before absorption by the plants.

[0038] A second preferred modality targets the ammonium nitrate molecule (NH4NO3) which is used as a source of nitrogen for plants. In this case, the frequency sequences will act on the set of atoms that form the chemical molecule in question. As a consequence of this action, the chemical bond between the atoms will be strengthened or weakened, causing a change in the energy requirement for the metabolization process of this molecule, that is, facilitating or hindering its transformation in the soil before absorption by the plants.

[0039] A third preferred modality targets the Ammonium Sulfate molecule [(NH4)2SO4] which is used as a source of nitrogen for plants. In this case, the frequency sequences will act on the set of atoms that form the chemical molecule in question. As a consequence of this action, the chemical bond between the atoms will be strengthened or weakened, causing a change in the energy requirement for the metabolization process of this molecule, that is, facilitating or hindering its transformation in the soil before absorption by the plants.

[0040] A fourth preferred modality targets the ammonium molecule (NH4), which is one of the main forms of nitrogen that plants can absorb. In this case, the frequency sequences will act on the set of atoms that form the chemical molecule in question. As a consequence of this action, the chemical bond between the atoms will be strengthened or weakened, causing a change in the energy requirement for the metabolization process of this molecule, that is, facilitating or hindering its transformation after absorption by the plants. [0041] A fifth preferred modality targets the Di-ammonium Phosphate (NH4)2HPO4 molecule, which is used as a source of phosphorus and nitrogen for plants. In this case, the frequency sequences will act on the set of atoms that form the chemical molecule in question. As a consequence of this action, the chemical bond between the atoms will be strengthened or weakened, causing a change in the energy requirement for the metabolization process of this molecule, that is, facilitating or hindering its transformation in the soil before absorption by the plants.

[0042] A sixth preferred modality targets the Phosphoric Acid molecule (H2PO4), which is one of the main forms of phosphorus that can be absorbed by plants. In this case, the frequency sequences will act on the set of atoms that form the chemical molecule in question. As a consequence of this action, the chemical bond between the atoms will be strengthened or weakened, causing a change in the energy requirement for the metabolization process of this molecule, that is, facilitating or hindering its transformation after absorption by the plants.

[0043] A seventh preferred embodiment targets the Potassium Chloride (KCl) molecule, which is used as a source of potassium for plants. In this case, the frequency sequences will act on the set of atoms that form the chemical molecule in question. As a consequence of this action, the chemical bond between the atoms will be strengthened or weakened, causing a change in the energy requirement for the metabolization process of this molecule, that is, facilitating or hindering its transformation in the soil before absorption by plants. [0044] An eighth preferred embodiment targets the Potassium Sulfate (K2SO4) molecule, which is used as a source of potassium for plants. In this case, the frequency sequences will act on the set of atoms that form the chemical molecule in question. As a consequence of this action, the chemical bond between the atoms will be strengthened or weakened, causing a change in the energy requirement for the metabolization process of this molecule, or that is, facilitating or hindering its transformation in the soil before absorption by plants.

[0045] A ninth preferred embodiment targets the CO2 molecule that is used by plants in the photosynthesis process. In this case, the frequency sequences will act on the set of atoms that form the chemical molecule in question. As a consequence of this action, the chemical bond between the atoms will be strengthened or weakened, causing a change in the energy requirement for the metabolization process of this molecule, that is, facilitating or hindering its participation in the physiological processes of plants.

Claims

1. Process for acting on elements present in the soil, water or air by means of extremely low frequency sequences characterized by identifying targets, drivers, objectives and objects for each action and consisting of 5 phases: Phase 1 - Determination of the plants' needs; Phase 2 - Determination of targets and drivers; Phase 3 - Communication with the frequency sequence generator; Phase 4 - Sending to the application device; Phase 5 - Database and Intelligence; whose implementation allows attenuating or accentuating the absorption of nutrients by plants. 2. Process for acting on elements present in the soil, water or air as per claim 1, characterized by having as target the urea molecule [CO(NH2)2], which is used as a source of nitrogen for plants, as a driver a combination of elements that make the target unique when present in the object which in this case is the soil or fertilizer, and as objective the approximation or distancing of its atoms, that is, the strengthening or weakening of the bonds between them. 3. Process for acting on elements present in the soil, water or air as per claim 1, characterized by having as target the ammonium nitrate molecule (NH4NO3) which is used as a source of nitrogen for plants, as director a combination of elements that make the target unique when present in the object which in this case is the soil or fertilizer, and as objective the approximation or distancing of its atoms, that is, the strengthening or weakening of the bonds between them. 4. Process for acting on elements present in the soil, water or air according to claim 1, characterized by having as target the ammonium sulfate molecule [(NH4)2SO4] which is used as a source of nitrogen for plants, as a driver a combination of elements that make the target unique when present in the object which in this case is the soil or fertilizer, and as objective the approximation or distancing of its atoms, that is, the strengthening or weakening of the bonds between them. 5. Process for acting on elements present in soil, water or air as per claim 1 characterized by having as target the ammonium molecule (NH4) which is one of the main absorbable forms of nitrogen by plants, as director a combination of elements that make the target unique when present in the object which in this case is the soil or fertilizer, and as objective the approximation or distancing of its atoms, that is, the strengthening or weakening of the bonds between them. 6. Process for acting on elements present in the soil, water or air as per claim 1, characterized by having as target the Diammonium Phosphate (NH4)2HPO4 molecule which is used as a source of phosphorus and nitrogen for plants, as a driver a combination of elements that make the target unique when present in the object which in this case is the soil or fertilizer, and as objective the approximation or distancing of its atoms, that is, the strengthening or weakening of the bonds between them. 7. Process for acting on elements present in the soil, water or air as per claim 1, characterized by having as target the Phosphoric Acid molecule (H2PO4) which is one of the main absorbable forms of phosphorus by plants, as director a combination of elements that make the target unique when present in the object which in this case is the soil or fertilizer, and as objective the approximation or distancing of its atoms, that is, the strengthening or weakening of the bonds between them. 8. Process for acting on elements present in the soil, water or air according to claim 1, characterized by having as a target the Potassium Chloride (KCI) molecule which is used as a source of potassium for plants, as a driver a combination of elements that make the target unique when present in the object which in this case is the soil or fertilizer, and as an objective the approximation or distancing of its atoms, that is, the strengthening or weakening of the bonds between them. 9. Process for acting on elements present in the soil, water or air according to claim 1, characterized by targeting the Potassium Sulfate (K2SO4) molecule, which is used as a source of potassium for plants, as a driver a combination of elements that make the target unique when present in the object, which in this case is the soil or fertilizer, and with the aim of bringing its atoms closer together or further apart, that is, strengthening or weakening the bonds between them. 10. Process for acting on elements present in the soil, water or air as per claim 1, characterized by having as target the CO2 molecule that is used by plants in the photosynthesis process, as director a combination of elements that make the target unique when present in the object which in this case is the air or soil or fertilizer, and as objective the approximation or distancing of its atoms, that is, the strengthening or weakening of the bonds between them.