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WO2025017538A1 - Compositions comprenant un mélange de lipo-chitooligosaccharide(s) et de phytostimulant(s), et leurs utilisations pour augmenter la croissance de plantes et les rendements des cultures - Google Patents

Compositions comprenant un mélange de lipo-chitooligosaccharide(s) et de phytostimulant(s), et leurs utilisations pour augmenter la croissance de plantes et les rendements des cultures Download PDF

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Publication number
WO2025017538A1
WO2025017538A1 PCT/IB2024/057050 IB2024057050W WO2025017538A1 WO 2025017538 A1 WO2025017538 A1 WO 2025017538A1 IB 2024057050 W IB2024057050 W IB 2024057050W WO 2025017538 A1 WO2025017538 A1 WO 2025017538A1
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Prior art keywords
plant
composition
lco
biostimulant
seed
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François LAMOUREUX
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11584022 Canada Inc
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11584022 Canada Inc
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators

Definitions

  • Gram-negative soil bacteria commonly named as rhizobia can establish root symbiosis with certain legumes and fix atmospheric nitrogen. These bacterial include genera of Rhizobium, Bradyrhizobium, Sinorhizobium, mesorhizobium, Phylorhizobium, and Azorhizobium.
  • LCOs Lipo-chitooligosaccharides
  • LCOs Lipo-chitooligosaccharides
  • LCOs are known to affect several host plant physiological processes, including inducing root hair deformation, inducing ontogeny of compete nodule structures, inducing cortical cell division and inducing the expression of host nodulin genes essential for infection thread formation LCOs have also been shown to activate defense-related enzymes.
  • Patent US 7,250,068 teaches increasing photosynthesis in plants by exposing the plants to lipo-chitooligosaccharides.
  • Patent publication US 2021/0235704 discloses combinations of lipo-chitooligosaccharides for enhancing plant growth.
  • Patent US 10,973,230 teaches the combined used of lipo- chitooligosaccharides and chitinous compounds for enhancing plant growth and yield.
  • Patents US 8,992,653 and US 11,560,340 disclose seed treatment methods comprising contacting seeds with LCO.
  • Patent publication US 2018/0235219 teaches compositions comprising lipo-chitooligosaccharides and microorganisms for enhancing plant growth.
  • Bacillus thuringiensis MS 20 produces Bacillin 20, a peptide that has been tested in the field for biostimulant efficacy against infections from Clavibacter michiganensis subsp. michiganensis (Cmm) in tomato (see International PCT application PCT/IB2024/052728, filed on March 21, 2024).
  • patent publication US 2003/0228679 discloses bacteria of the species Bacillus subtilis (i.e., NEB4 and NEB5) and of the species Bacillus thurigiensis (i.e., NEB17) which can promote plant growth.
  • B. thuringiensis NEB17 produces a polypeptide named Thuricin-17, a polypeptide which has been suggested of having a positive impact on plant growth, at least under certain conditions (see international PCT patent publication WO 2008/138129; Marion P. et al., Agronomy for sustainable development 35 (2015): 749-757; Gray, E. J., and Donald L. Smith, Soil biology and biochemistry 37, no.3 (2005): 395-412; Smith D.
  • compositions and methods allowing to modulate and/or take advantage of plant-to-bacteria and bacteria-to-plant communications and molecular signaling in agriculture.
  • compositions and methods allowing to increase agricultural production in commercial and large-scale agriculture.
  • compositions combining both lipo- chitooligosaccharides (LCOs) and a plant biostimulant such as Bacillin 20 in a single mixture, the combination providing more benefits on plant growth, including crop yield, than each individual components used individually.
  • LCOs lipo- chitooligosaccharides
  • Bacillin 20 Bacillin 20
  • compositions capable of increasing agricultural production or crop yield are contacted with the plants (e.g., roots or seed).
  • seed coating compositions that can provide benefits such as improving or enhancing growth parameters such as crop yields.
  • the invention relates to a composition for increasing plant growth comprising a combined mixture of at least one lipo-chitooligosaccharides (LCO) and one or more plant biostimulant.
  • LCO lipo-chitooligosaccharide
  • the invention relates to a composition comprising at least one lipo-chitooligosaccharide (LCO) and one or more plant biostimulant, wherein said at least one LCO and said one or more plant biostimulant are present in amounts effective to synergistically enhance one or more growth parameters of a plant when said composition is applied to said plant.
  • the invention relates to a treated seed comprising a plant seed that is at least partially coated with a seed treatment composition, wherein said seed treatment composition comprises at least one lipo-chitooligosaccharide and one or more one or more plant biostimulant.
  • the invention relates to a method for enhancing plant growth, comprising applying at least one lipo-chitooligosaccharide (LCO) and one or more plant biostimulant to said plant in amounts effective to synergistically enhance one or more growth parameters of said plant.
  • LCO lipo-chitooligosaccharide
  • the invention relates to a method for enhancing plant growth, comprising applying at least one lipo-chitooligosaccharide (LCO) and one or more plant biostimulant to a plant seed in amounts effective to synergistically enhance one or more growth parameters of the plant that grows from said seed.
  • LCO lipo-chitooligosaccharide
  • the invention relates to a method for increasing crop yield, comprising contacting plants with at least one lipo-chitooligosaccharide (LCO) and one or more plant biostimulant, wherein said contacting synergistically enhance crop yield of plants contacted with both compounds, as compared to contacting plants with only the at least one lipo-chitooligosaccharide (LCO) or only the one or more plant biostimulant.
  • LCO lipo-chitooligosaccharide
  • LCO lipo-chitooligosaccharide
  • the invention relates to a method of increasing corn yield, said method comprising applying at least one lipo-chitooligosaccharide (LCO) and one or more plant biostimulant to corn seed in amounts effective to synergistically increase the yield of corn grown from said corn seed, and wherein said at least one lipo- chitooligosaccharide and said one or more plant biostimulant are applied to said corn seed prior to planting.
  • LCO lipo-chitooligosaccharide
  • the invention relates to a kit for stimulating plant growth, comprising: (i) a first container comprising a concentrated solution of at least one LCO; and (ii) a second container comprising a concentrated solution of one or more plant biostimulant.
  • the invention relates to a kit for stimulating plant growth, comprising: (i) a first container comprising a mixture of at least one LCO and one or more plant biostimulant; and (ii) a second container comprising at least one of a herbicide, an insecticide, a fungicide, a bactericide, and a nutrient.
  • the invention relates to the use of at least one LCO and one or more plant biostimulant for stimulating plant growth, wherein said at least one LCO and one or more plant biostimulant are applied on a plant simultaneously or concurrently.
  • LCOs Lipo-chitooligosaccharides
  • Plant biostimulants are natural and environmentally friendly substances with positive effects on plant growth. Plant biostimulants can enhance plant metabolism and physiology when applied in small doses.
  • plant-microbe interactions has largely been unexplored and unexploited for the improvement and optimization of plant production, relief of plant stress, and disease resistance.
  • the present invention proposes to modulate and/or take advantage of plant- to-bacteria and bacteria-to-plant communications and molecular signaling for agriculture.
  • the present invention concerns the demonstration that contacting lipo-chitooligosaccharide(s) (LCOs) with plant biostimulant(s) significantly increases plant growth, and particularly crop yield.
  • LCOs lipo-chitooligosaccharide(s)
  • Applicant has found that synergistic effects can be obtained by combining lipo- chitooligosaccharide(s) (LCOs) with plant biostimulant(s).
  • a composition of the present invention comprises a mixture of at least one LCO and one or more plant biostimulant (e.g., Bacillin 20) synergistically increased yield of corn.
  • lipo-chitooligosaccharides Prior to the present invention, no one had ever combined lipo- chitooligosaccharides (LCOs) and a peptidic plant biostimulant such as Bacillin 20 in a single mixture, nor tested the effect of such combination on plants, let alone agricultural production or yield. The surprising benefits associated with such combination thus open the way to numerous applications in the field of agriculture.
  • LCOs lipo-chitooligosaccharides
  • LCO therefore relates to a bacteria-to-plant signal molecule which induces the formation of nodules in legumes and enables the symbiotic bacteria to colonize same.
  • LCOs are lipo-chitooligosaccharide signal molecules, acting as phytohormones, comprising an oligosaccharide moiety having a fatty acid condensed at one of its end.
  • LCOs from B. japonicum have been characterized in U.S. Pat. Nos. 5,175,149 and 5,321,011, which are incorporated herein by reference.
  • LCOs are also commercially available from Novozymes (Milwaukee, WI, USA) under the brand names RatchetTM, TorqueTM IF, and B360TM.
  • the present invention encompasses the uses of one or more of these known LCOs.
  • the present invention encompasses compositions comprising various LCOs and it is not limited the LCOs exemplified hereinafter.
  • LCOs obtained from B. japonicum it is envisioned that other LCOs may also be effective as that of the LCOs exemplified herein.
  • effective LCOs can be synthetic or naturally occurring.
  • LCOs may be isolated and/or purified or purchased commercially (see above).
  • LCOs may be isolated and/or purified from a species of rhizobia which is capable of entering into a nitrogen fixation relationship with a plant including but not limited to, Bradyrhizobium spp., Rhizobium spp., Sinorhizobium spp. Azorhizobium spp.
  • the LCO is obtained from Bradyrhizobium japonicum strain 532C (see Soulemanov et al., Microbial Research, Vol. 157, 1, 2022, p. 25-28).
  • the chemical name of the LCO is Nod Bj-V (C18:1, MeFuc).
  • plant biostimulant refers to a biological agent or combination of agents, which application to a plant enhances one or more metabolic and/or physiological processes of the plant or part thereof (e.g., stress mitigation, respiration, photosynthesis, nucleic acid uptake, ion uptake and/or nutrient delivery).
  • the plant biostimulant is a peptide produced by a bacterium, for instance Bacillus thuringiensis or Bacillus subtilis, including but not limited to Bacillus thuringiensis MS 20, Bacillus thurigiensis NEB17, Bacillus subtilis NEB4 and Bacillus subtilis NEB5.
  • the plant biostimulant consists of Bacillin 20, a peptide produced the bacterium Bacillus thuringiensis strain 20 (BtMS20). BtMS20 was deposited at the International Depositary Authority of Canada (IDAC) on November 3 rd , 2020 under Accession No.301020-01.
  • the Bacillin 20 peptide comprises the amino acid sequence DWTCWSCLVCAACSVELLNLVTAATGASTAS (SEQ ID NO:1).
  • the plant biostimulant consists of Thuricin 17, a peptide produced by Bacillus thuringiensis strain NEB17 (the strain was deposited at the International Depositary Authority of Canada, Winnipeg, Manitoba, Canada, on March 27, 2003 under Accession No. 301020-01.
  • the Thuricin 17 peptide comprises the amino acid sequence DWTCWSCLVCAACSVELLNLVTAATGASTAS (SEQ ID NO:1).
  • the plant biostimulant consists of a peptide produced by Bacillus subtilis strain NEB4 or a peptide produced by Bacillus subtilis strain NEB5.
  • the B. subtilis strain NEB4 was deposited at the International Depositary Authority of Canada, Winnipeg, Manitoba, Canada, in 2003 under Accession No. AF406704.
  • the B. subtilis strain NEB5 was deposited at the International Depositary Authority of Canada, Winnipeg, Manitoba, Canada, in 2003 under Accession No. AF406705.
  • the plant biostimulant may comprises an isolated peptide comprising SEQ ID NO: 1 as defined hereinabove, as well as any fragments of SEQ ID NO: 1, or any isolated protein, fusion protein or peptide comprising SEQ ID NO: 1, and having substantially similar biological activity as the peptide of SEQ ID NO: 1, particularly in displaying plant biostimulant activity and/or in improving crop yield in combination with LCO(s) as described herein.
  • the plant biostimulant may comprise an amino acid sequence having at least 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% identity over the entire length of SEQ ID NO:1.
  • Sequence identity may be determined using any suitable software, for instance by using the BLASTTM algorithms (e.g., BLASTp), available through the internet at the National Center for Biotechnology Information (NCBI).
  • BLASTTM algorithms e.g., BLASTp
  • NCBI National Center for Biotechnology Information
  • the peptide may comprise a fragment of SEQ ID NO:1, the fragment comprising at least 25, 26, 27, 27, 28, or 29 consecutive amino acids of SEQ ID NO:1.
  • the fragment should have substantially identical plant biostimulant activity and/or be able to improve crop yield in combination with LCO(s), in a substantially identical manner to than peptide of SEQ ID NO:1 as described herein.
  • Plant biostimulants useful in practicing the methods of the invention including Bacilin 20, Thuricin 17, peptide(s) from B.
  • subtilis strain NEB4 or B. subtilis strain NEB5 and/or related peptides may be obtained in a number of ways. For example, any polypeptide of interest may be screened either sequentially in either order, or simultaneously, for a desired biological activity, including but not limited to plant biostimulant activity and/or improving crop yield in combination with LCO(s) as described herein.
  • plant biostimulant polypeptides are obtained from a bacterial species that express the polypeptides.
  • suitable bacterial strain may be cultured under conditions sufficient for expression of the polypeptide and the polypeptide recovered from the culture medium.
  • the polypeptide may be purified e.g., by chromatography (e.g., high-performance liquid chromatography), gel electrophoresis, filtration, dialysis, precipitation, centrifugation, etc. or combinations thereof. It may also be possible to obtain the biostimulant peptides of interest by chemical synthesis and/or by overexpression in a bacterial system.
  • Bacilin 20 is produced by the bacterium BtMS20 and it is obtainable therefrom (e.g., purified).
  • the bacterium BtMS20 is cultured in King’s B liquid medium at 28 ⁇ 2 °C on an orbital shaker for at least 96 hours, the entire culture is extracted with of n-butanol (shaking for 30 min keeping overnight at room temperature and the separated butanol phase is collected and evaporated under vacuum at 50 °C using a rotary evaporator.
  • the dried extract is resuspended in 20% acetonitrile and loaded on PrepSep C18TM cartridge (Fisher Scientific, cat# 03-251-292) and fractionated using 20, 30, 35, 40, 60, 70 and 100% acetonitrile (in water, v/v).
  • the present invention also encompasses bacterial extracts, such as extracts from BtMS20, comprising one or more Bacilin 20 polypeptides as defined herein.
  • bacterial extracts such as extracts from BtMS20, comprising one or more Bacilin 20 polypeptides as defined herein.
  • the present invention further encompasses compositions comprising peptides (e.g., Bacilin 20 and/or fragment or peptides deriving therefrom as defined herein), proteins and bacterial strain (e.g., BtMS20) as defined herein.
  • the present invention further encompasses bacterial extracts, such as extracts from Bacillus thurigiensis NEB17 comprising one or more Thuricin 17 polypeptides as defined herein, or extracts from B. subtilis strain NEB4 or from B. subtilis strain NEB5, compositions comprising such peptides (e.g., isolated peptides and/or fragment deriving therefrom), and proteins and bacterial strains (e.g., NEB17, NEB4 and NEB5) as defined herein.
  • bacterial extracts such as extracts from Bacillus thurigiensis NEB17 comprising one or more Thuricin 17 polypeptides as defined herein, or extracts from B. subtilis strain NEB4 or from B. subtilis strain NEB5, compositions comprising such peptides (e.g., isolated peptides and/or fragment deriving therefrom), and proteins and bacterial strains (e.g., NEB17, NEB4 and NEB5) as defined herein.
  • a particular aspect of the invention concerns compositions combining both, at least one lipo-chitooligosaccharide (LCO) with one or more biostimulant.
  • LCO lipo-chitooligosaccharide
  • Applicant has found that synergistic effects on plant growth (e.g., crop yield) can be obtained when combining such compounds.
  • the present invention encompasses compositions comprising at least one lipo-chitooligosaccharide (LCO) and at least one biostimulant in amounts effective to synergistically enhance one or more growth parameters of a plant when said composition is applied to the plant, e.g., the seed from which said plant is grown.
  • the term “amounts effective to synergistically enhance one or more growth parameters of the plant” refers to a quantity which is sufficient to result in enhancement of the growth of the plant as compared to growth of a control-treated (or untreated) crop plant. In particular embodiments, enhancement of growth is displayed as an increased crop yield. Examples of growth parameters are provided hereinafter. [00051] As used herein, “synergistically enhance”, “synergistic enhancement” or “synergistic combination” and grammatical variants thereof) refers to a combined effect that is more than a simple additive effect.
  • the combined use of at least one lipo-chitooligosaccharide (LCO) and one or more plant biostimulant provides benefit(s) to the plant that is(are) greater than the sum of benefit(s) for the at least one lipo-chitooligosaccharide (LCO) and the one or more plant biostimulant when used separately.
  • LCO lipo-chitooligosaccharide
  • the Examples hereinafter demonstrate that corn seed treatment with a mixture comprising LCO + Bacillin 20 provided an increase in corn yield that was greater than simple a coating of LCO alone or a coating of Bacillin 20 alone, thereby suggesting a synergistic effect when using both compounds in combination.
  • both compounds are applied to the plant(s) concurrently or contemporaneously, for instance by contacting the plant(s) with a mixture comprising both compounds. It is also conceivable to obtains synergistic effects if the compounds are applied to or contacted with the plant(s) (e.g., seed) in sequential order (e.g., one before or after the other). [00052]
  • the yield-promoting activity of the compositions of the invention may be observable over a broad range of concentrations.
  • the LCO(s) is(are) present in a concentrated liquid mixture at a concentration between about 10 -7 M to about 10 -9 M and it is used as a diluted liquid solution (e.g., a dilutive factor of 10X, 50X, 100X, 500X, 1000X or more) to be applied on plants at a concentration of about 10 -7 M to about 10 -12 M, preferably about 10 -9 M to about 10 -12 M, and more preferably about 10 -8 M to about 10 -10 M.
  • a dose of about 8500 mg of LCO is used to treat seeds for over 40000 ha of corn (i.e., about 25 kg of corn seeds/ha).
  • a dose of about 8500 mg of LCO is used to treat seeds for over 20,000 ha of soybean (i.e., about 70-100 kg of soybean seeds/ha). In embodiments, a dose of about 6000 mg to about 22 000 mg of LCO is used to treat about 750,000 kg to about 2,500,000 kg of corn seeds.
  • the plant biostimulant is present in a concentrated liquid mixture at a concentration between about 10 -6 M to about 10 -10 M and it is used in a diluted liquid solution (e.g., a dilutive factor of 10X, 50X, 100X, 500X, 1000X or more) and applied on plants at a concentration of about 10 -7 M to about 10 -13 M, preferably about 10 -8 M to about 10 -12 M, and more preferably about 10 -9 M to about 10 -11 M.
  • a dose of about 200 mg of Bacilin 20 (in an isolated form or as part of a bacterial extract) is used to treat seeds for over 40,000 ha of corn (i.e., about 25 kg of corn seeds/ha).
  • compositions of the invention may contain dilutents, adjuvants, excipients, carriers, wetting agents, surfactants, anti-freezing agents, etc. known in the art as suitable for inclusion in plant growth and/or crop yield promoting compositions.
  • the invention provides a composition comprising a LCO and a plant biostimulant as described herein, and a carrier or diluent.
  • compositions may be in, for example, solid (such as powdered) or liquid form.
  • the composition is sterilized for greater stability and the components remain stable and active for more than 2 years.
  • carrier means an agronomically acceptable carrier, a oil compatible carrier, a seed-compatible carrier and/or a foliar-compatible carrier.
  • An "agronomically acceptable carrier” means any material which can be used to deliver the actives (e.g., LCO(s) + biostimulant(s), etc.), to a plant, plant part or plant growing locus (e.g., foliage, seed, soil).
  • the term “soil compatible carrier” means any material which can be added to a soil without causing/having substantial adverse effect on plant growth, soil structure, soil drainage, or the like.
  • the term “seed-compatible carrier” means any material which can be added to a seed without causing/having an adverse effect on the seed, the plant that grows from the seed, seed germination, or the like.
  • the term “foliar-compatible carrier” means any material which can be added to a plant or plant part without causing/having an adverse effect on the plant, plant part, plant growth, plant health, or the like.
  • plant can refer to a whole plant, any part thereof, or a cell or tissue culture derived from a plant.
  • the term “plant” may refer to any of whole plants, plant components or organs (e.g., leaves, stems, roots, etc.), plant tissues, plant cells and seeds.
  • plant includes all naturally occurring and non-naturally occurring plant populations, including, agricultural, horticultural and silvicultural plants.
  • the term "plant” encompasses plants obtained by conventional plant breeding and optimization methods (e.g., marked-assisted selection) and plants obtained by genetic engineering (e.g., transgenic), including cultivars protectable and not protectable by plant breeders' rights.
  • the composition of the present invention is preferably applied in an amount effective for increasing plant growth. In embodiments, the composition of the present invention is applied in an effective amount.
  • Terms like "effective amount,” “effective concentration,” and “effective dosage” refer to an amount, concentration or dosage that is sufficient to cause a desired effect (e.g., enhanced plant growth).
  • the terms “enhanced growth”, “enhanced plant growth”, and the like refer to an improvement in one or more characteristics or parameters of plant growth and/or plant development as compared to one or more control plants (e.g., a plant germinated from an untreated seed or an untreated plant).
  • Exemplary plant growth/development characteristics or parameters include, but are not limited to, biomass, height, leaf length, leaf mass, leaf number, leaf surface area, leaf volume, nutrient uptake, root area, root diameter, root length, root mass, root nodulation, root number, root surface area, root volume, nitrogen fixation, seed germination, seedling emergence, shoot diameter, shoot length, shoot mass, shoot number, shoot surface area, shoot volume, plant spread, plant survival rate, and plant or crop yield.
  • the terms “increasing crop yield” or “improving yield” and the like refer to an improvement in one or more characteristics of plant yield or production, as compared to one or more control plants (e.g., a plant germinated from an untreated seed or an untreated plant).
  • Exemplary plant yield/production characteristics include, but are not limited to, increased biomass, increased fruit number increased bolls, increased seed number or size, or a combination thereof as measured by bushels per acre, increased root number, increased root mass, increased root volume, increased leaf area, increased plant stand, increased plant vigor, improved disease resistance, reduced or inhibited pathogen infestation, faster seedling emergence (e.g., enhanced emergence), faster germination, (e.g., enhanced germination), or combinations thereof) when compared to an untreated plant or untreated plant part.
  • the increase of crop yield associated with using the composition of the invention is more than 10, 20, 30, 40, 50, 60, or 70%, compared to a crop from control plants (e.g., UTC).
  • the at least one LCO is applied to plant seeds at a rate of about 0.8 ng per kg to about 8000 ng per kg, or about 8 ng per kg to about 800 ng per kg, or about 80 ng per kg to about 400 ng per kg.
  • the biostimulant is applied to plant seeds at a rate of about 0.1 ng per kg to about 15000 ng per kg, or about 1 ng per kg to about 1500 ng per kg, or about 10 ng per kg to about 150 ng per kg.
  • the composition comprising both the LCO and biostimulant can be applied in an amount that ranges between about 0.1 to about 10 liter/A, or between about 0.5 to about 5 liter/A, or between about 1 to about 3 liter/A
  • the absolute value of the amount/concentration/dosage that is sufficient to cause desired effect(s) may be affected by factors such as size (e.g., area, total acreage, etc.) of land being treated, the type and condition of soils, the species, variety and/or condition of the plant, the environment (e.g., whether the plant is in a pot, a greenhouse, a field), etc.
  • a composition in accordance with the present invention may be applied by any suitable means or method including, but not limited to, seed coating, application to seeds prior to and/or during planting, root inoculation, rood dipping, soil drenching, soil application (e.g., injection), foliar spray (e.g., high- or low-pressure spraying), etc.
  • the composition may be applied either in a solid form (e.g., as a free-flowing powder) or liquid form (such as in an aqueous carrier).
  • the composition may also be applied to a delivery vehicle, wherein the delivery vehicle serves as a means of transporting the LCO and biostimulant to the soil, plant, seed, field, etc.
  • the compounds or composition can be applied to a delivery vehicle (e.g., a particle, a polymer, or a substrate) to be used in filtration systems for the treatment of irrigation water.
  • the compounds or composition is to be applied to a polymer as a wetting agent and/or gel that releases water as needed.
  • Seed coating and root dipping prior to planting are two preferred techniques. Seed coating, root irrigation, root dipping, soil drenching, foliar applications such as spray treatments of leaves, etc.
  • the invention also concerns plant seeds pre-treated with a composition of the invention, e.g., the composition is applied to the seeds in advance of planting (e.g., a seed coating that has occurred 1 week, 2 weeks, 4 weeks, 2 months, 3 months, 4 months, or 6 months, or 1 year or more prior to planting).
  • the invention relates to plant seeds treated and/or coated with a composition as defined herein.
  • the material coated on dry seeds is effective for at least 1 year or at least 2 years.
  • the invention also related to foliar applications with a composition of the invention.
  • foliage refers to those portions of a plant that normally grow above the ground, including, but not limited to, leaves, stalks, stems, flowers, fruiting bodies, and fruits.
  • foliar application “foliarly applied” and grammatical variations thereof, refer to the application of one or more active ingredients to the foliage of a plant (e.g., to the leaves of the plant).
  • Application may be effected by any suitable means, including, but not limited to, spraying the plant with a composition comprising the active ingredient(s).
  • the active ingredient(s) is/are applied to the leaves, stems and/or stalk of the plant and not to the flowers, fruiting bodies or fruits of the plant.
  • the compositions of the invention can be applied at a particular time, or one or more times, depending on parameters including, but not limited to, soil types, soil conditions, environmental conditions, time of planting, plant susceptibility, etc.
  • the method for increasing crop yield comprises contacting plants with at least one lipo-chitooligosaccharide (LCO) and one or more plant biostimulant.
  • LCO lipo-chitooligosaccharide
  • the method for enhancing plant growth comprises applying at least one lipo-chitooligosaccharide (LCO) and one or more plant biostimulant to a plant seed in amounts effective to synergistically enhance one or more growth parameters of the plant that grows from said seed.
  • LCO lipo-chitooligosaccharide
  • the at least one lipo-chitooligosaccharide (LCO) and the one or more plant biostimulant can be applied by a variety of techniques including, but not limited to, high- or low-pressure spraying, coating, immersion, and injection. Once treated, seeds can be planted in natural or artificial soil and cultivated using conventional procedures to produce plants.
  • a composition in accordance with the present invention may additionally or alternatively be applied to the growing environment of the plant or seed rather than directly to the plant or seed.
  • growing environment is meant the area sufficiently proximal to the plant or seed (such as to the soil adjacent to the plant or seed) that the composition and/or individual components can affect have a desired beneficial effect on the plant. If the composition is applied to the soil, it may be applied before, during or after planting.
  • compositions in accordance with the present invention may also find additional useful preventive and/or curative applications for various agricultural crops (e.g., cereals), fruits (e.g., small fruits, citrus), vegetables, grass, turf, ornamental plants, etc.
  • agricultural crops e.g., cereals
  • fruits e.g., small fruits, citrus
  • vegetables e.g., grass, turf, ornamental plants, etc.
  • the present invention has been demonstrated in the Examples using corn, the invention should not be so limited. Indeed, it will be clear to a person skilled in the art to which the present invention pertains that other types of plants may respond similarly to the combined LCO+biostimulant application, by displaying an increase in crop yield thereof. [00076] Therefore, the compositions and methods of the invention are not limited to use with any particular plant or plant-type.
  • the invention is used for enhancing growth of one or more of monocot, dicot, C3 plants and C4 plants.
  • plants with which the compositions and methods of the invention may be practiced include, without limitation: cereals (e.g., wheat, rye, maize, corn, sorghum, oat, barley, bulgur, rice, millet, and canola), rice (black rice, brown rice, red rice), tree nuts (e.g., almonds, cashews, pecans, pistachios, etc.) ground nuts (e.g., peanuts), fruits (e.g., tomato), vegetables (e.g., potato), oil seed crops (e.g.
  • cereals e.g., wheat, rye, maize, corn, sorghum, oat, barley, bulgur, rice, millet, and canola
  • rice black rice, brown rice, red rice
  • tree nuts e.g., almonds, cashews, pecans, pist
  • canola soybeans, cotton
  • forage crops e.g., alfalfa and clover
  • plants of lesser agricultural importance e.g., lupine, sainfoin, trefoil, and even some small tree species
  • horticultural tree species e.g., peach, apple, plum, pear, mango
  • forestry tree species e.g., spruce, pine, fir, maple, oak, poplar
  • flowers cannabis, grass, turf, and ornamental plants.
  • the term "agriculturally beneficial agent” refers to any agent (e.g., chemical or biological agent) or combination of agents the application of which causes and/or provides a beneficial and/or useful effect in agriculture (e.g., enhanced plant growth).
  • Such agents include, but not limited to, agriculturally beneficial microorganisms (e.g., rhizobacteria), biostimulants, nutrients, chemical fertilizers, flavonoids, pesticides (e.g., acaricides, fungicides, gastropodicides, herbicides, insecticides, nematicides, rodenticides, and virucides), and plant signal molecules such as lipo-chitooligosaccharides, chitooligosaccharides, chitinous compounds, flavonoids, jasmonic acid or derivatives thereof, linoleic acid or derivatives thereof, linolenic acid or derivatives thereof, karrikins, etc.
  • agriculturally beneficial microorganisms e.g., rhizobacteria
  • biostimulants e.g., acaricides, fungicides, gastropodicides, herbicides, insecticides, nematicides, rodenticides, and virucides
  • the methods of the invention further comprise applying at least one of a herbicide, an insecticide, a fungicide, a bactericide, and a nutrient.
  • agriculturally beneficial microorganisms refer to a microorganism having at least one agriculturally beneficial property (e.g., the ability to produce an agriculturally beneficial agent, such as a plant signaling molecule described herein; to enhance nutrient and/or water uptake; to promote and/or enhance nitrogen fixation; to enhance growth; to enhance seed germination; to enhance seedling emergence; to increase seed number or size; to break the dormancy or quiescence of a plant; etc.).
  • compositions of the invention may be used, and the methods of the invention practiced, wherever plants are grown. Accordingly, the present invention encompasses uses of the compositions and methods as defined herein in a variety of plant environments such as fields, greenhouse facilities, vertical farms, urban greening systems, hydroponic systems and laboratories.
  • the compositions may be used with plants that are grown at temperatures above 30°C, at which temperatures nitrogen fixing rhizobacteria are generally most active, or also at low temperatures, such as at an average daily root zone temperature below 28, 26, 24, 22, 20, 18, 16, 14, 12, or 10°C.
  • Examples of potentially useful herbicides include, but are not limited to, RoundupTM, atrazine, 2,4-D (Weed-B-GonTM), dicamba (BanvelTM), imazethapyr (PursuitTM), metolachlor (DualTM), S-metolachlor (Dual II MagnumTM), pendimenthalin (ProwlTM), clethodim (SelectTM), triclopyr (GarlonTM), clopyralid (StingerTM), Fluro StarTM, flufosinate (LibertyTM), halosulfuron (PermitTM), isoxaben (GalleryTM), sethoxydim (PoastTM), bentazon (BasagranTM), pyraflufen-ethyl (EpicTM), fomesafen (ReflexTM), trifluralin (TreflanTM), mesotrione (CallistoTM), flumioxazin (Valor), dicamba
  • Examples of potentially useful insecticides include, but are not limited to, pyrethoids, pyrethins, neonicotinoids, organophosphates, carbamates, spinosad, imidacloprid, fipronil, malathion, permethrin, cypermethrin, and chlorpyrifos.
  • Examples of potentially useful bactericide include, but are not limited to, copper sulfate, zinc sulfate, peracetic acid, quaternary ammonium compounds, streptomycin, kasugamycin, tetracycline hydrochloride, phyton 27TM, aureobasidium pullulans, ActigardTM, and Agri- strepTM.
  • Examples of potentially useful nutrients include, but are not limited to, nitrogen, phosphorus, potassium, sulphur, magnesium, calcium and other plant micronutrients.
  • Examples of potentially useful fungicides include, but are not limited to, copper fungicide that includes copper sulfate, copper sulfate pentahydrate, copper hydroxide, copper oxychloride sulfate, cuprous oxide, copper octanoate, and mixtures thereof.
  • compositions and methods of the invention may provide numerous benefits.
  • using the composition of the provide one or more of the following benefits: ⁇ mitigating negative effects and stress induced by application of herbicide(s) and pesticide(s) to plants and crops (e.g., by at least 2%, 3%, 4%, 5%, 6%, 7%, 10%, 15%, 20%, 25%, 30% or more); ⁇ increasing crop yields (e.g., by at least 2%, 3%, 4%, 5%, 6%, 7%, 10%, 15%, 20%, 25%, 30% or more); ⁇ increasing plant growth (e.g., by at least 3%, 4%, 5%, 6%, 7%, 10%, 15%, 20%, 25% or more); ⁇ increasing leaf area, greenness, number and/or biomass (e.g., by at least 3%, 4%, 5%, 6%, 7%, 10%, 15%, 20%, 25% or more); ⁇ stimulating the plant root system (e.g., by at least 3%, 4%, 5%, 6%, 7%, 10%, 15%, 20%, 25% or more
  • benefit(s) is(are) observed or determined by comparing plant(s) contacted or not with the two compounds or with the composition of the invention. In embodiments, benefit(s) is(are) observed when comparing plant(s) contacted with the composition versus plant(s) contacted with a reference treatment (e.g., UTC or compounds individually).
  • a reference treatment e.g., UTC or compounds individually.
  • seeds are treated prior to planting and the reference treatments consist of a LCO and a plant biostimulant (e.g., Bacillin 20), both applied individually as a seed coating.
  • the benefit(s) is(are) observed or determined about 3, 5, 7, 14, 21, 28, 35, 42, 60 days, or about 3 months, or about 4 months after contacting the plant with the two compounds or with the composition of the invention.
  • Packages/Kits [00085]
  • the composition in accordance with the invention may be provided in the form of a package or kit containing the individual components (i.e., LCO(s) and plant biostimulant(s) separately) or a composition comprising both components, and optionally additional elements such as instructions for uses in promoting plant growth.
  • the kit comprises separate containers such as bottles, e.g., a bottle comprising the LCO(s) and another bottle comprising the plant biostimulant(s) (e.g., Bacillin 20), and optional instructions for mixing both product prior use.
  • the LCO(s) and biostimulant(s) are provided in a concentrated format (either as a mixture or as individual components) for dilution prior use (e.g., 10x, 50x, 100x, 500x, 1000x, 10000x concentrate).
  • the kit comprises a first container having a composition comprising both LCO(s) and the plant biostimulant(s) in a concentrated or diluted form, and a second container comprising at least one agriculturally beneficial ingredient as defined above.
  • a concentrate of LCO(s), a concentrate of the plant biostimulant(s) or a concentrated mixture thereof may, as necessary, be formulated with suitable agricultural excipients such as agronomically acceptable carriers, polymers, wetting agents, surfactants, anti-freezing agents, etc.
  • suitable agricultural excipients such as agronomically acceptable carriers, polymers, wetting agents, surfactants, anti-freezing agents, etc.
  • Example 1 Combination of lipo-chitooligosaccharides (LCOs) and plant biostimulant Bacillin 20 increases agricultural production
  • LCOs lipo-chitooligosaccharides
  • Bacillin 20 increases agricultural production
  • LCOs lipo-chitooligosaccharides
  • UTC untreated check
  • Bacillin 20 [00092] The Bacillin 20 used for the field trials consisted of a Bacillin 20 aqueous stock solution. Bacillin 20 was purified from Bacillus thuringiensis MS20 strain. The process used to isolate Bacillin 20 involved the following steps: 1) microbial culture production; 2) centrifugation and membrane filtration; 3) butanol extraction of organic metabolites; 4) evaporation of butanol and concentration of extract; 5) fractionation of the organic extract using acetonitrile solvent; 6) purification of Bacillin 20 using HPLC; and 7) mass spectrometry analysis of Bacillin 20 to confirm identity.
  • step 1) Bacillus thuringiensis MS20 was cultured in Kings B medium incubated in a shaker incubator at 125 rpm and at 30 °C for at least 96 h.
  • step 2) bacterial mass was removed by centrifugation and membrane filtration to obtain a cell- free supernatant.
  • the butanol extraction of step 3) was carried out by mixing and vigorous shaking the microbial cell free supernatant with 1-butanol and phase partitioning of 1- butanol.
  • step 4) the butanol was evaporated using a rotary evaporator and the organic extract was suspended in 20% acetonitrile.
  • the organic extract was fractionated using solid phase extraction (SPE) cartridges to remove undesirable and "junk” compounds, retaining only active fractions of Bacillin 20 for further purification.
  • SPE solid phase extraction
  • Bacillin 20 was purified conducting high performance liquid chromatography (HPLC) using a gradient of acetonitrile/water to a target of 85-95% purity of Bacillin 20.
  • HPLC high performance liquid chromatography
  • mass spectrometry analysis was done on a purified sample of Bacillin 20 to confirm identity of the peptide using molecular weight.
  • the purified Bacillin 20 (i.e., about 85 to about 95% purity) was diluted in phosphate buffered saline to obtain a stock solution comprising a 10 -9 M concentration of Bacillin 20.
  • the stock solution of Bacillin 20 was diluted 100-fold in water to a final ready- to-use diluted 10 -11 M working solution which was used for seed treatment.
  • Lipo-chitooligosaccharides [00095] The lipo-chitooligosaccharides (LCOs) were obtained from Bradyrhizobium japonicum 532C strain in accordance with the procedure described by Soulemanov A, Prithiviraj B, Carlson RW, Jeyaretnam B, Smith DL.2002. Isolation and characterization of the major nod factor of Bradyrhizobium japonicum strain 532C. Microbiological Research.157: 25-8.
  • Nod factor peak was identified by comparing with the retention time of the standard (Nod Bj-V (C18:1, MeFuc)) of strain USDA110. The concentration of the Nod factor was calculated by the comparison of the area of the peak of the standard, at a known concentration.
  • Freeze Drying Eluted fractions containing high concentrations of pure Nod factor were freeze dried and used for mass spectrometry and biological activity tests.
  • Mass spectrometry Molecular weight identification was done by LC-MS analysis. The results confirmed that the major Nod factor (Nod Bj-V, C18:1, MeFuc) was present in the samples.
  • Zea Mays L corn seeds (variety P1718VYHR) were treated prior to planting with working solutions comprising: LCO alone; Bacillin 20 alone; a mixture of both LCO + Bacillin 20; or just water (control or UTC).
  • the seed treatment comprised soaking the corn seeds in the different solutions followed by air drying before planting.
  • the seeds from the different treatments were planted in adjacent plots of 10 ft wide x 40 feet long for an individual plot area of 400 square feet. Each seed treatment was repeated in six different plots for a total area of 2400 square feet per treatment.
  • planting was on June 6, 2022 and emergence was observed about 6 days later (June 12, 2022).
  • Table 1 Corn Response to LCO & Bacillin 20 subsequent to seed coating (Experiment A) Treatments Yield % yield increase (Bushel per acre) compared to UTC UTC 121 0 LCO 121 0.00% Bacillin 20 125 3.31% LCO + Bacillin 20 128 5.79% [000111]
  • Table 2 Corn Response to LCO & Bacillin 20 subsequent to seed coating (Experiment B) Treatments Yield % yield increase (Bushel per acre) compared to UTC UTC 117 0 LCO 121 3.42% Bacillin 20 123 5.13% LCO + Bacillin 20 132 12.82% [000112] The data of Table 1 and Table 2 clearly demonstrate a positive effect on corn yield of corn seed treated with either LCO or Bacillin 20 when compared to untreaded seeds (UTC).
  • Example 2 Combination of lipo-chitooligosaccharides (LCOs) and plant biostimulant Bacillin 20 increases soybean yield
  • LCOs lipo-chitooligosaccharides
  • FCOs plant biostimulant Bacillin 20 increases soybean yield
  • Field trials were conducted in Brazil to evaluate the effectiveness of Bacillin 20 alone, LCO alone, and a mixture of Bacillin 20 and LCO, as growth-promoting inoculants, applied via seed treatment, on growth and yield variables, in soybean crop.
  • Primavera do Leste, MT this trial took place at the Ceres Consultoria Agronômica on Rodovia MT 130, km 30, at an altitude of 589 meters. The climate there was tropical with distinct wet and dry seasons, an average annual temperature of 23.7 °C, and annual precipitation of 1,868 mm.
  • the soil was classified as Red Latosol with a sandy clay loam texture.
  • Barreiras, BA the trial occurred at the Experimental Station of Multicrop Research and Development on Rodovia BA 460, km 9, at an altitude of 725 meters.
  • the climate was tropical, with an average annual temperature of 23.3 °C and precipitation of 1,273 mm, making it the driest among the study locations.
  • the soil was also a Red Latosol but with a sandy texture.
  • Campo Alegre de Goiás, GO This was hosted in the Central-West region, at an altitude of 868 meters. This area had a tropical climate with dry winters, an average annual temperature of 21.2 °C, and rainfall of 1,525 mm.
  • the soil was a dystrophic red Latosol with a clayey texture.
  • Gurupi TO: this trial was held at the Multcrop experimental station. The climate there was tropical with dry winters, featuring a good distribution of rainfall, an average annual temperature of 26.1 °C, and annual precipitation of 1,776 mm. The soil was classified as a Dystrophic Red-Yellow Latosol with a clay loam texture.
  • Treatments and Management The trials involved four treatments: a control without any treatment, application of the test product Bacillin 20 (referred to as ABIO), application of the LCO from Bradyrhizobium japonicum strain 532C (see above) and a combination of Bacillin 20 and LCO.
  • ABIO test product Bacillin 20
  • LCO from Bradyrhizobium japonicum strain 532C
  • the experimental design was a randomized complete block design (RCBD) with four treatments and six replications. Each plot measured 54 m2 and was spaced 0.5 meters apart to avoid contamination between treatments. The plant population was set at 300,000 seeds per hectare. [000123] Dosage for the treatments in the trials was as follows: 1. Control: Only water was applied to the seeds. 2. Bacillin 20 (referred to as ABIO): The test product Bacillin 20 (ABIO) was seed applied at a dose of 0.50 mL/kg of seeds. 3. LCO: The LCO was also seed applied at a dose of 0.50 mL/kg of seeds. 4.
  • ABIO Bacillin 20
  • LCO The LCO was also seed applied at a dose of 0.50 mL/kg of seeds.
  • Bacillin 20 (ABIO) + LCO A combination of Bacillin 20 (ABIO) and LCO was seed applied, with each product dosed at 0.50 mL/kg of seeds, resulting in a total application volume of 1.00 mL/kg of seeds.
  • the application method involved coating the products onto the seeds using at the rate of 10 mL/kg of seeds, which included both water and the products. After treating the seeds with the treatments the seeds were dried and prepared for sowing.
  • the trials assessed various growth and production parameters of the soybean plants. These included: • Germination: The percentage of seeds that successfully germinated, determined through laboratory tests. • Emergency Speed Index (ESI): A measure of the speed at which seeds emerged, calculated using the Maguire (1962) formula.
  • Table 4 Soybean vegetative growth variables in different locations [000132] The following results can be observed from the data presented in Table 5 (important numbers are highlighted): A) ABIO+LCO 0.8% increase in Yield (Primavera do Leste/MT) B) ABIO+LCO 1.1% increase in Number of Pods per Plant (Barreiras/BA) C) ABIO+LCO 1.5% increase in Yield (Barreiras/BA) D) ABIO+LCO 2.6% increase in Number of Pods per Plant (Campo Alegre de Goiàs/GO) E) ABIO+LCO 4.2% increase in Number of Grains per Pod (Campo Alegre de Goiàs/GO) F) ABIO+LCO 2.1% increase in 1000 grain weight(g) (Campo Alegre de Goiàs/GO) G) ABIO+LCO 5.6% increase in Yield (Campo Alegre de Goiàs/GO) H) ABIO+LCO 2.9% increase in Number of Pod
  • Example 3 Combination of lipo-chitooligosaccharides (LCOs) and plant biostimulant Bacillin 20 increases corn crop development and yield
  • LCOs lipo-chitooligosaccharides
  • Field trials were conducted in Brazil to evaluate the effectiveness of Bacillin 20 alone, LCO alone, and a mixture of Bacillin 20 and LCO, as growth-promoting compounds applied via seed treatment on corn crop development and yield. The trials were conducted at two locations: Primavera do Leste/MT and Gurupi/TO.
  • Treatments and Management The treatments included: 1) a control with only water applied at a spray volume of 10 mL/kg seed, 2) ABIO at 0.82 mL/kg seeds, applied with water at 10 mL/kg seed, 3) LCO at 0.82 mL/kg seeds, and 4) a combination of ABIO and LCO, each at 0.82 mL/kg seeds.
  • the experimental design consisted of randomized blocks with four treatments and six replications. A minimum of 60,000 seeds per hectare were used, with three seeds per linear meter, resulting in a minimum plot size of 54.0 m2 (12 rows, 9 meters long, spaced 0.5 meters apart), and 0.5-meter borders between plots. Each plot contained 324 seeds.
  • Measured Parameters included a germination test post-inoculation and pre-seeding, emergency speed index (ESI) from sowing to the end of emergence over 6 meters linear per plot, and crop stand at the V6 growth stage over 10 meters linear per plot. Plant vigor was visually assessed at the V6 and V10 growth stages, graded from 1 to 10. Plant height, fresh and dry mass of the aerial part, and fresh and dry root mass were measured at the V10 growth stage with five plants per plot. At harvest, average cob length and diameter, number of grains per cob, number of rows of grains per cob, number of grains per row, 1000 grain weight, and yield (all corrected to 13% moisture) were assessed. Application and planting took place between January and March 2024.

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Abstract

L'invention concerne des procédés et des compositions comprenant une combinaison de lipo-chitooligosaccharide(s) (LCO) et de phytostimulant(s) pour augmenter la croissance des plantes, et en particulier le rendement des cultures. Des avantages synergiques sont obtenus lors de la combinaison du ou des lipo-chitooligosaccharides avec le ou les phytostimulants.
PCT/IB2024/057050 2023-07-20 2024-07-19 Compositions comprenant un mélange de lipo-chitooligosaccharide(s) et de phytostimulant(s), et leurs utilisations pour augmenter la croissance de plantes et les rendements des cultures Pending WO2025017538A1 (fr)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025217017A1 (fr) 2024-04-08 2025-10-16 Novozymes A/S Compositions et procédés pour augmenter la disponibilité du phosphore

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008138129A1 (fr) * 2007-05-16 2008-11-20 The Royal Institution For The Advancement Of Learning/Mcgill University Thuricine 17 permettant de favoriser la croissance végétale et la résistance des plantes à une maladie et plantes transgéniques
WO2016014386A1 (fr) * 2014-07-23 2016-01-28 Novozymes Bioag A/S Compositions contenant des lipo-chitooligosaccharides et des microorganismes et destinées à améliorer la croissance des plantes
WO2017205258A1 (fr) * 2016-05-26 2017-11-30 Novozymes Bioag A/S Bacillus et lipo-chito-oligosaccharide pour améliorer la croissance de plantes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008138129A1 (fr) * 2007-05-16 2008-11-20 The Royal Institution For The Advancement Of Learning/Mcgill University Thuricine 17 permettant de favoriser la croissance végétale et la résistance des plantes à une maladie et plantes transgéniques
WO2016014386A1 (fr) * 2014-07-23 2016-01-28 Novozymes Bioag A/S Compositions contenant des lipo-chitooligosaccharides et des microorganismes et destinées à améliorer la croissance des plantes
WO2017205258A1 (fr) * 2016-05-26 2017-11-30 Novozymes Bioag A/S Bacillus et lipo-chito-oligosaccharide pour améliorer la croissance de plantes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025217017A1 (fr) 2024-04-08 2025-10-16 Novozymes A/S Compositions et procédés pour augmenter la disponibilité du phosphore

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