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WO2025090132A3 - Procédés et kits de détection d'endotoxine - Google Patents

Procédés et kits de détection d'endotoxine

Info

Publication number
WO2025090132A3
WO2025090132A3 PCT/US2024/028332 US2024028332W WO2025090132A3 WO 2025090132 A3 WO2025090132 A3 WO 2025090132A3 US 2024028332 W US2024028332 W US 2024028332W WO 2025090132 A3 WO2025090132 A3 WO 2025090132A3
Authority
WO
WIPO (PCT)
Prior art keywords
endotoxin
kits
methods
detection
detecting endotoxin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/028332
Other languages
English (en)
Other versions
WO2025090132A2 (fr
WO2025090132A9 (fr
Inventor
Andrew DIX
Alexia BACHIR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technolohgies Corp
Original Assignee
Life Technolohgies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technolohgies Corp filed Critical Life Technolohgies Corp
Publication of WO2025090132A2 publication Critical patent/WO2025090132A2/fr
Publication of WO2025090132A9 publication Critical patent/WO2025090132A9/fr
Publication of WO2025090132A3 publication Critical patent/WO2025090132A3/fr
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96433—Serine endopeptidases (3.4.21)
    • G01N2333/96441—Serine endopeptidases (3.4.21) with definite EC number
    • G01N2333/96463—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/50—Lipopolysaccharides; LPS

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Est décrit un procédé amélioré basé sur un lysat d'amibocytes pour la détection d'endotoxine. Le procédé ne nécessite qu'une seule étape d'incubation et permet l'utilisation de divers volumes d'entrée d'échantillon, ce qui permet une plus grande flexibilité tout en maintenant une détection sensible d'endotoxine à des concentrations allant de 0,01 à 10 unités d'endotoxine (EU)/ml. Sont également décrits des kits pour réaliser le procédé de détection d'endotoxine.
PCT/US2024/028332 2023-05-12 2024-05-08 Procédés et kits de détection d'endotoxine Pending WO2025090132A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202363501972P 2023-05-12 2023-05-12
US63/501,972 2023-05-12
US202463641556P 2024-05-02 2024-05-02
US63/641,556 2024-05-02

Publications (3)

Publication Number Publication Date
WO2025090132A2 WO2025090132A2 (fr) 2025-05-01
WO2025090132A9 WO2025090132A9 (fr) 2025-06-19
WO2025090132A3 true WO2025090132A3 (fr) 2025-07-24

Family

ID=95071417

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/028332 Pending WO2025090132A2 (fr) 2023-05-12 2024-05-08 Procédés et kits de détection d'endotoxine

Country Status (1)

Country Link
WO (1) WO2025090132A2 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5334703A (en) * 1989-12-12 1994-08-02 Chromonogenix Ab Chromogenic substrate
WO2018132562A1 (fr) * 2017-01-11 2018-07-19 Lonza Walkersville, Inc. Lysat d'amibocytes de limule clarifié sans coagulogène

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4406832A (en) 1981-09-03 1983-09-27 Mallinckrodt, Inc. Peptide-type substrates useful in the quantitative determination of endotoxin
US5569587A (en) 1986-04-18 1996-10-29 Carnegie Mellon University Method for labeling and detecting materials employing luminescent arysulfonate cyanine dyes
US5268486A (en) 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5627027A (en) 1986-04-18 1997-05-06 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5866366A (en) 1997-07-01 1999-02-02 Smithkline Beecham Corporation gidB
AU2018282868B2 (en) 2017-06-16 2023-10-19 Duke University Resonator networks for improved label detection, computation, analyte sensing, and tunable random number generation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5334703A (en) * 1989-12-12 1994-08-02 Chromonogenix Ab Chromogenic substrate
WO2018132562A1 (fr) * 2017-01-11 2018-07-19 Lonza Walkersville, Inc. Lysat d'amibocytes de limule clarifié sans coagulogène

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "Pierce(TM) Chromogenic Endotoxin Quant Kit", 18 January 2023 (2023-01-18), pages 1 - 6, XP093278423, Retrieved from the Internet <URL:https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017902_ChromogenicEndotoxinQuantKit_UG.pdf> *
ANONYMOUS: "Qubit (TM) Endotoxin Detection Assay Kit - User Guide", 22 May 2023 (2023-05-22), pages 1 - 24, XP093278625, Retrieved from the Internet <URL:https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0029395-QubitEndotoxinDetectAssay-UG-EN.pdf> *
LINDSAY G K ET AL: "Single-step, chromogenic Limulus amebocyte lysate assay for endotoxin", JOURNAL OF CLINICAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 27, no. 5, 1 May 1989 (1989-05-01), pages 947 - 951, XP002691773, ISSN: 0095-1137 *

Also Published As

Publication number Publication date
WO2025090132A2 (fr) 2025-05-01
WO2025090132A9 (fr) 2025-06-19

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