[go: up one dir, main page]

WO2025088131A1 - Procédé de lutte contre des champignons phytopathogènes à l'aide de jawsamycine - Google Patents

Procédé de lutte contre des champignons phytopathogènes à l'aide de jawsamycine Download PDF

Info

Publication number
WO2025088131A1
WO2025088131A1 PCT/EP2024/080247 EP2024080247W WO2025088131A1 WO 2025088131 A1 WO2025088131 A1 WO 2025088131A1 EP 2024080247 W EP2024080247 W EP 2024080247W WO 2025088131 A1 WO2025088131 A1 WO 2025088131A1
Authority
WO
WIPO (PCT)
Prior art keywords
jawsamycin
plant
botrytis
composition
plants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2024/080247
Other languages
English (en)
Inventor
Stuart Bagley
Santiago VALDES
Olivier Loiseleur
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Crop Protection AG Switzerland
Original Assignee
Syngenta Crop Protection AG Switzerland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Crop Protection AG Switzerland filed Critical Syngenta Crop Protection AG Switzerland
Publication of WO2025088131A1 publication Critical patent/WO2025088131A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/28—Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/067—Pyrimidine radicals with ribosyl as the saccharide radical

Definitions

  • the present invention relates to a method for controlling phytopathogenic fungi using jawsamycin.
  • Jawsamycin is a natural compound, indicated as FR-900848, that is produced by Streptoverticillium or Streptomyces bacteria.
  • the compound was first discovered in 1990 by Yoshida et al (1990), J. of Antibiotics, p. 748-754.
  • Yoshida et al (1990) discloses that the compound was active against Aspergillus niger, Mucorrouxianaus,, Aureobasidium, Penicillium chrysogenum,, Fusarium oxysporum, Sclerotinia arachidis and Trichophyton species.
  • JP2101006 discloses that jawsamycin was active against apple leaf spot Alternaria and against cucumber Botrytis grey mold.
  • Fu et. al. (2020), Nature Communications 11-3387, discloses that jawsamycin was active against some yeasts, fungi such as Aspergillus sp., Fusarium oxysporum, Scedosporium sp, and some Mucorales fungi. Fu et al. (1990) suggests that jawsamycin would have a broad-spectrum fungicidal activity, however, there is no teaching or suggestion which fungi are affected, nor whether by jawsamycin would be fu ngicidally active on which planta.
  • the present invention relates to a method of controlling or preventing phytopathogenic fungi belonging to Glomerella lagenarium, Magnaporthe grisea, Mycosphaerella arachidis, Phaeosphaeria nodorum, Pyrenophora teres, Venturia inaequalis, Botrytis eliptica, Botrytis tulipae, Corynespora cassiicola, Cercospora kikuchii, and/or Cercospora sojina, on a plant or on plant propagation material thereof, the method comprising applying to the plant, plant propagation material or locus thereof, a composition comprising jawsamycin.
  • the present invention relate to the use of jawsamycin for controlling or preventing phytopathogenic fungi, wherein the fungus is selected from Glomerella lagenarium, Magnaporthe grisea, Mycosphaerella arachidis, Phaeosphaeria nodorum, Pyrenophora teres, Venturia equalis, Botrytis eliptica, Botrytis tulipae, Corynespora cassiicola, Cercospora kikuchii, and Cercospora sojina.
  • the fungus is selected from Glomerella lagenarium, Magnaporthe grisea, Mycosphaerella arachidis, Phaeosphaeria nodorum, Pyrenophora teres, Venturia equalis, Botrytis eliptica, Botrytis tulipae, Corynespora cassiicola, Cercospora kikuchii, and Cercospora sojin
  • fungicidally effective amount where used means the quantity of such a compound or combination of such compounds that is capable of producing an effect on the growth of fungi. Controlling or modifying effects include all deviation from natural development, such as killing, retardation and the like, and prevention includes barrier or other defensive formation in or on a plant to prevent fungal infection. Controlling or preventing means reducing infestation by phytopathogenic fungi to such a level that an improvement is demonstrated.
  • a fungicidally effective amount of jawsamycin comprises 0.1 to 500 ppm, preferably 0.5 to 250 ppm of jawsamycin.
  • jawsamycin was surprisingly effective to control the fungi Glomerella lagenarium, Magnaporthe grisea, Mycosphaerella arachidis, Phaeosphaeria nodorum (syn. Parastagonospora nodorum), Pyrenophora teres, Venturia inaequalis, Botrytis eliptica, Botrytis tulipae, Corynespora cassiicola, Cercospora kikuchii, and I or Cercospora sojina. Accordingly, jawsamycin has a surprising fungicidal activity against these fungi.
  • jawsamycin showed very little or no activity against oomycetes, such as Phytophthora infestans, Pythium ultimum, and Plasmopora viticola, Basidiomycetes, such as Puccinia recondita, and Rhizoctonia solan! (syn. Thanatephorus cucumeris) and the Ascomycetes Blumeria graminis, and Uncinula necator. Accordingly, it appears the teaching of Fu, Y. et al. in Nat Commun 11 , 3387 (2020) cannot be applied to all fungi.
  • Jawsamycin is a known compound and has a structural formula according to Formula (la)
  • the jawsamycin of formula (la) is an oligocyclopropyl-containing natural product, also noted as FR- 900848.
  • Jawsamycin is a polyketide comprising a 5'-amino-5'-deoxy-3,4,5,6-tetrahydrouridine in which one of the hydrogens of the amino group is substituted by a (1 E,3E)-1- [(1 R,1 'R, 1 "R,1 "'R,2S,2'R,2"R,2"'S)-2"'- ⁇ (E)-2-[(1 R,2R)-2-methylcyclopropyl]ethenyl ⁇ [1 ,1 ':2',1 ":2",1 quater(cyclopropan)]-2-yl]-5-oxopenta-1 ,3-dien-5-yl group.
  • jawsamycin is C32H43N3O6, comprising a 5'-amino-5'-deoxy-5, 6-dihydrouridine with an unsaturated fatty acid comprising four serial and one isolated cyclopropane rings.
  • IUPAC name of jawsamycin is (2E,4E)-N-[[(2R,3S,4R,5R)-5-(2,4-dioxo-1 ,3-diazinan-1-yl)-3,4- dihydroxyoxolan-2-yl]methyl]-5-[(1 S,2R)-2-[(1 R,2R)-2-[(1 R,2R)-2-[(1 R,2S)-2-[(E)-2-[(1 R,2R)-2- methylcyclopropyl]ethenyl]cyclopropyl]cyclopropyl]cyclopropyl]cyclopropyl]cyclopropyl]penta-2,4-dienamide.
  • a microorganism expressing a compound according to formula (la) may be a Streptoverticillium and/or a Streptomyces, preferably Streptoverticillium fervens, and/or Streptomyces luteoverticillatus, or their spores, as these microorganisms express the compounds of formula (la) as metabolites.
  • a particularly suitable route to produce jawsamycin may be by cultivating Streptoverticillium fervens and isolating jawsamycin, for instance according to the methods disclosed in Masaru et al, The Journal of Antibiotics, 1990, Volume 43, Issue 7, Pages 748-754.
  • Congeners of jawsamycin are compounds lb and Ic according to following formulae: These congeners according to formula lb) and Ic) are expressed by the above microbial cultures in varying, but usually small or negligible amounts.
  • the method according to the present invention comprises applying a composition comprising Jawsamycin to a plant, plant propagation material or locus thereof. Applying jawsamycin to a plant may be performed by spraying, dusting or any suitable other means.
  • a suitable method of controlling or preventing an infestation of crop plants by Glomerella lagenarium, Magnaporthe grisea, Mycosphaerella arachidis, Phaeosphaeria nodorum, Pyrenophora teres, Venturiainia inaequalis, Botrytis eliptica, Botrytis tulipae, Corynespora cassiicola, Cercospora kikuchii, and / or Cercospora sojina comprises the application of an agrochemical composition comprising jawsamycin is foliar application. The frequency of application and the rate of application will depend on the risk of infestation by the phytopathogenic fungi. However, jawsamycin can also penetrate the plant through the roots via the soil by drenching the locus of the plant with a liquid formulation, or by applying the compounds in solid form to the soil, e.g. in granular form (soil application).
  • the propagation material can be treated with a composition comprising jawsamycin before planting: seed, for example, can be dressed before being sown. Jawsamycin can also be applied to grains (coating), either by impregnating the seeds in a liquid formulation or by coating them with a solid formulation.
  • the composition can also be applied to the planting site when the propagation material is being planted, for example, to the seed furrow during sowing.
  • the method according to the present invention comprises applying compositions, wherein the composition may be employed in any conventional form, for example in the form of a twin pack, a powder for dry seed treatment (DS), an emulsion for seed treatment (ES), a flowable concentrate for seed treatment (FS), a solution for seed treatment (LS), a water dispersible powder for seed treatment (WS), a capsule suspension for seed treatment (CF), a gel for seed treatment (GF), an emulsion concentrate (EC), a suspension concentrate (SC), a suspo-emulsion (SE), a capsule suspension (CS), a water dispersible granule (WG), an emulsifiable granule (EG), an emulsion, water in oil (EG), an emulsion, oil in water (EW), a micro-emulsion (ME), an oil dispersion (OD), an oil miscible flowable (OF), an oil miscible liquid (OL), a soluble concentrate (SL), an ultra-low volume suspension (SU), an ultra-low
  • compositions may be produced in conventional manner, e.g., by mixing the active ingredients with at least one appropriate inert formulation adjuvant (for example, diluents, solvents, fillers, and optionally other formulating ingredients such as surfactants, biocides, anti-freeze, stickers, thickeners, and compounds that provide adjuvancy effects).
  • inert formulation adjuvant for example, diluents, solvents, fillers, and optionally other formulating ingredients such as surfactants, biocides, anti-freeze, stickers, thickeners, and compounds that provide adjuvancy effects.
  • conventional slow-release formulations may be employed where long lasting efficacy is intended.
  • Particularly formulations to be applied in spraying forms such as water dispersible concentrates (e.g.
  • wettable powders and granules may contain surfactants such as wetting and dispersing agents and other compounds that provide adjuvancy effects, e.g. the condensation product of formaldehyde with naphthalene sulphonate, an alkylarylsulphonate, a lignin sulphonate, a fatty alkyl sulphate, and ethoxylated alkylphenol and an ethoxylated fatty alcohol.
  • surfactants such as wetting and dispersing agents and other compounds that provide adjuvancy effects, e.g. the condensation product of formaldehyde with naphthalene sulphonate, an alkylarylsulphonate, a lignin sulphonate, a fatty alkyl sulphate, and ethoxylated alkylphenol and an ethoxylated fatty alcohol.
  • Any suitable plant may be treated to prevent or control infestation by Glomerella lagenarium, Magnaporthe grisea, Mycosphaerella arachidis, Phaeosphaeria nodorum, Pyrenophora teres, enturiainia inaequalis, Botrytis eliptica, Botrytis tulipae, Corynespora cassiicola, Cercospora kikuchii, and / or Cercospora sojina.
  • a suitable plant includes ornamental plants such as trees, shrubs, flowers and houseplants, and also fruits, vegetables, herbs and forages.
  • the plant is selected from vegetables, such as cucumber, tomato, fruits, such as apple, pear, grapes, cereals, such as barley, wheat, corn, peanut, rice and soya.
  • a plant in method as disclosed herein for controlling or preventing infestation by Glomerella lagenarium comprises or is cucumber.
  • Glomerella lagenarium was effectively controlled at a fungicidally effective amount of jawsamycin of 0.1 to 500 ppm , preferably 0.2 to 300 ppm, preferably 0.3 to 200 ppm.
  • a plant in method as disclosed herein for controlling or preventing infestation by Magnaporthe grisea comprises or is rice.
  • Magnaporthe grisea was surprisingly effectively controlled at a fungicidally effective amount of jawsamycin of 1 to 500 ppm , preferably 2 to 300 ppm, preferably 5 to 200 ppm.
  • a plant in method as disclosed herein for controlling or preventing infestation by Mycosphaerella arachidis comprises or is peanut.
  • Mycosphaerella arachidis was surprisingly effectively controlled on planta at a fungicidally effective amount of jawsamycin of 20 to 500 ppm, preferably 30 to 300 ppm, preferably 40 to 250 ppm.
  • a plant in method as disclosed herein for controlling or preventing infestation by Pyrenophora teres comprises or is barley. Pyrenophora teres was surprisingly effectively controlled at a fungicidally effective amount of jawsamycin of 0.5 to 500 ppm , preferably 1 to 300 ppm, preferably 2to 200 ppm.
  • a plant in method as disclosed herein for controlling or preventing infestation by Venturia inaequalis comprises or is fruit, preferably apple. Venturia inaequalis was surprisingly effectively controlled at a fungicidally effective amount of jawsamycin of 0.1 to 500 ppm , preferably 0.2 to 300 ppm, preferably 0.3 to 200 ppm.
  • a plant in method as disclosed herein for controlling or preventing infestation by Phaeosphaeria nodorum comprises or is wheat. Phaeosphaeria nodorum is effectively controlled at a fungicidally effective amount of jawsamycin of 50 to 500 ppm, preferably 60 to 400 ppm
  • plants refers to all physical parts of a plant, including seeds, seedlings, saplings, roots, tubers, stems, stalks, foliage, and fruits.
  • a plant as used herein also includes useful plants.
  • useful plants is to be understood as also including useful plants that have been rendered tolerant to herbicides.
  • useful plants is to be understood as also including useful plants which have been so transformed by the use of recombinant DNA techniques that they are capable of synthesising one or more selectively acting toxins, such as are known, for example, from toxin-producing bacteria, especially those of the genus Bacillus.
  • plant propagation material is understood to denote generative parts of the plant, such as seeds, which can be used for the multiplication of the latter, and vegetative material, such as cuttings or tubers, for example potatoes.
  • vegetative material such as cuttings or tubers, for example potatoes.
  • seeds in the strict sense
  • roots in the strict sense
  • fruits in the tubers
  • bulbs rhizomes
  • parts of plants there can be mentioned for example seeds (in the strict sense), roots, fruits, tubers, bulbs, rhizomes and parts of plants.
  • Germinated plants and young plants which are to be transplanted after germination or after emergence from the soil may also be mentioned. These young plants can be protected before transplantation by a total or partial treatment by immersion.
  • plant propagation material is understood to denote seeds.
  • a composition comprising jawsamycin is an agriculturally acceptable composition.
  • locus means fields in or on which plants are growing, or where seeds of cultivated plants are sown, or where seed will be placed into the soil. It includes soil, seeds, and seedlings, as well as established vegetation.
  • composition stands for the various mixtures or combinations of jawsamycin, for example in a single “ready-mix” form, in a combined spray mixture composed from separate formulations of the single active ingredient components, such as a “tank-mix”, and in a combined use of the single active ingredients when applied in a sequential manner, i.e. one after the other with a reasonably short period, such as a few hours or days.
  • composition comprising jawsamycin in a method according to the present invention preferably is an agriculturally acceptable composition.
  • the composition further comprises at least one agriculturally acceptable carrier, additive, formulation adjuvant, and / or a surfactant.
  • Suitable carriers and adjuvants can be solid or liquid and are substances useful in formulation technology, e.g. natural or regenerated mineral substances, solvents, dispersants, wetting agents, tackifiers, thickeners, binders or fertilizers.
  • the composition comprises one or more further biologically active compound(s), preferably one or more further pesticide, such as fungicides, insecticides, or herbicides.
  • further pesticidal components are known and are referred to hereinabove by their so-called ISO common name or their IUPAC name.
  • Typical pesticidal components are included in the Pesticide Manual Online (“E-pesticide Manual") by the British Crop Production Council and in the Compendium of Pesticide Common Names by Alan Wood.
  • jawsamycin is applied at a rate of from 5 to 2000 g a.i./ ha, particulary 1 g a.i./ha to 1000 g a.i. I ha, preferably at a rate 5 g a.i./ha to 500 g a.i./ha, preferably at a rate 1 g a.i./ha to 250 g a.i./ha.
  • the abbreviation “a.i.” stands for “active ingredient”
  • the active ingredient comprises or is jawsamycin
  • jawsamycin is applied at a rate of, 0.001 to 50 g of a.i. per kg of plant propagation material, in particular per kg of seeds.
  • jawsamycin is applied at a rate of, 0.01 to 40 g, preferably at a rate of 0.1 to 30 g of a.i. per kg of plant propagation material.
  • the present invention also relates to the use of jawsamycin for controlling or preventing phytopathogenic fungi, wherein the fungus is selected from Glomerella lagenarium, Magnaporthe grisea, Mycosphaerella arachidis, Phaeosphaeria nodorum, Pyrenophora teres, Venturia inaequalis, Botrytis eliptica, Botrytis tulipae, Corynespora cassiicola, Cercospora kikuchii, and Cercospora sojina.
  • the fungus is selected from Glomerella lagenarium, Magnaporthe grisea, Mycosphaerella arachidis, Phaeosphaeria nodorum, Pyrenophora teres, Venturia inaequalis, Botrytis eliptica, Botrytis tulipae, Corynespora cassiicola, Cercospora kikuchii,
  • jawsamycin for controlling or preventing phytopathogenic fungi is on a plant or on plant propagation material.
  • the method and use of the present invention excludes methods and uses for the treatment of the human or animal body by surgery or therapy.
  • Jawsamycin employed in the examples was produced by cultivating Streptoverticillium fervens and isolating jawsamycin according to the methods disclosed in Masaru et al, The Journal of Antibiotics, 1990, Volume 43, Issue 7, Pages 748-754.
  • Example 1 In vitro liquid culture assays.
  • Conidia or mycelial fragments from the fungus / oomycetes listed in Table 1 were mixed into nutrient broth (potato dextrose broth (PDB) / Vogel’s minimal media (Microbial Genetics Bulletin 13:42-43, 1956)).
  • a 10 pL solution of jawsamycin and DMSO was dispensed into a well of a 96-well microtiter plate, before 90 pL of the nutrient broth containing the conidia or mycelial fragments was added to the well.
  • the assay plate was then incubated at 24 °C and assessed photometrically at 620 nm after 2 to 7 days incubation.
  • the efficacy of jawsamycin was assessed by comparing test wells to non-treated inoculated wells and non-inoculated wells and allocating efficacy on a scale of 0-100 %.
  • Leaf discs efficacy was assessed on a scale of 0 - 100 % by comparing test discs to non-treated, inoculated leaf discs representing 0 % control and leaf discs treated with a known standard that completely controls the pathogen representing 100 % control.
  • Tomato leaf disks were placed on water agar in multiwell plates (24-well format) and sprayed with test solutions. After drying, the leaf disks are inoculated with a spore suspension of the fungus. After appropriate incubation the activity of a compound was assessed 4 dpi (days post inoculation) as preventive fungicidal activity. Dose range: 200-22 ppm.
  • Plasmopara viticola downy mildew of grapevine
  • Grape vine leaf disks were placed on agar in multiwell plates (24-well format) and sprayed with test solutions. After drying, the leaf disks are inoculated with a spore suspension of the fungus. After appropriate incubation the activity of a compound was assessed 7 dpi (days post inoculation) as preventive fungicidal activity. Dose range: 200-22 ppm.
  • Barley leaf segments were placed on agar in multiwell plates (24-well format) and sprayed with test solutions. After drying, the leaf disks are inoculated with spores of the fungus. After appropriate incubation the activity of a compound was assessed 7 dpi (days post inoculation) as preventive fungicidal activity. Dose range: 66-22 ppm.
  • Barley leaf segments were placed on agar in multiwell plates (24-well format) and sprayed with test solutions. After drying, the leaf disks are inoculated with a spore suspension of the fungus. After appropriate incubation the activity of a compound was assessed 4 dpi (days post inoculation) as preventive fungicidal activity. Dose range: 200-22 ppm.
  • Wheat leaf segments were placed on agar in multiwell plates (24-well format) and sprayed with test solutions. After drying, the leaf disks are inoculated with a spore suspension of the fungus. After appropriate incubation the activity of a compound was assessed 8 dpi (days post inoculation) as preventive fungicidal activity. Dose range: 200-22 ppm.
  • Phaeosphaeria nodorum (syn. Parastagonospora nodorum, Glume blotch):
  • Wheat leaf segments were placed on agar in multiwell plates (24-well format) and sprayed with test solutions. After drying, the leaf disks are inoculated with a spore suspension of the fungus. After appropriate incubation the activity of a compound was assessed 4 dpi (days post inoculation) as preventive fungicidal activity. Dose range: 200-22 ppm.
  • Rice leaf segments were placed on agar in multiwell plates (24-well format) and sprayed with test solutions. After drying, the leaf disks are inoculated with a spore suspension of the fungus. After appropriate incubation the activity of a compound was assessed 5 dpi (days post inoculation) as preventive fungicidal activity. Dose range: 200-22 ppm.
  • test plants cv. Arina 1-week old wheat plants cv. Arina were sprayed in a spray chamber with the formulated test compound diluted in water.
  • the test plants were inoculated by spreading powdery mildew spores over them in an inoculation chamber two days after application.
  • the inoculated test plants were incubated at 20° C I 18°C (day/night) and 60% rh in a greenhouse and the percentage leaf area covered by disease was assessed when an appropriate level of disease appeared on untreated check plants (7 - 9 days after application).
  • Glomerella lagenarium (Colletotrichum lagenarium) on cucumber with a preventative application.
  • 1-week old cucumber plants cv. Wisconsin were sprayed in a spray chamber with the formulated test compound diluted in water.
  • the test plants were inoculated by spraying them with a spore suspension one day after application. After an incubation period of 30 h in darkness at 23° C and 100% rh, the inoculated test plants were kept at 23° C / 21 ° C (day/night) and 70% rh in a greenhouse. The percentage leaf area covered by disease was assessed when an appropriate level of disease appeared on untreated check plants (6 - 8 days after application).
  • Magnaporthe grisea (Pyricularia oryzae) on rice with a preventative application.
  • 3-weeks old rice plants cv. Koshihikari were sprayed in a spray chamber with the formulated test compound diluted in water.
  • the test plants were inoculated by spraying them with a spore suspension two days after application.
  • the inoculated test plants were incubated at 25° C and 95% rh and the percentage leaf area covered by disease was assessed when an appropriate level of disease appeared on untreated check plants (7 - 9 days after application).
  • Mycosphaerella arachidis (Cercospora arachidicola) on peanut with a preventative application 3-weeks old peanut plants cv. Georgia Green were sprayed in a spray chamber with the formulated test compound diluted in water.
  • the test plants were inoculated by spraying them with a spore suspension on their lower leaf surface one day after application. After an incubation period of 4 days under a plastic hood at 23° C and 100% rh, the inoculated test plants were kept at 23° C 1 20° C (day/night) and 70% rh in a greenhouse. The percentage leaf area covered by disease was assessed when an appropriate level of disease appeared on untreated check plants (12 - 14 days after application).
  • Phytophthora infestans on potato with a preventative application 2-weeks old potato plants cv. Bintje are sprayed in a spray chamber with the formulated test compound diluted in water.
  • the test plants were inoculated by spraying them with a sporangia suspension 2 days after application.
  • the inoculated test plants were incubated at 18° C with 14 h light/day and 100 % rh in a growth chamber and the percentage leaf area covered by disease was assessed when an appropriate level of disease appeared on untreated check plants (5 - 7 days after application).
  • test plants plants were inoculated by spraying a sporangia suspension on their lower leaf surface one day after application.
  • the inoculated test plants were incubated at 22° C and 100% rh in a greenhouse and the percentage leaf area covered by disease was assessed when an appropriate level of disease appeared on untreated check plants (6 - 8 days after application).
  • Puccinia recondita f. sp. tritici on wheat with a preventative application Puccinia recondita f. sp. tritici on wheat with a preventative application.
  • 2-weeks old wheat plants cv. Arina were sprayed in a spray chamber with the formulated test compound diluted in water.
  • the test plants were inoculated by spraying them with a spore suspension one day after application. After an incubation period of 1 day at 20° C and 95% rh, the inoculated test plants were kept at 20° C / 18° C (day/night) and 60% rh in a greenhouse. The percentage leaf area covered by disease was assessed when an appropriate level of disease appeared on untreated check plants (12 - 14 days after application).
  • test plants cv. Regina were sprayed in a spray chamberwith the formulated test compound diluted in water.
  • the test plants were inoculated by spraying them with a spore suspension 2 days after application.
  • the inoculated test plants were incubated at 20° C and 95% rh and the percentage leaf area covered by disease was assessed when an appropriate level of disease appeared on untreated check plants (5 - 7 days after application).
  • 3-weeks old apple seedlings were sprayed in a spray chamber with the formulated test compound diluted in water.
  • the test plants were inoculated by spraying them with a spore suspension 1 day after application. After an incubation period of 2 days at 20° C and 95% rh, the inoculated test plants were placed at 20°C/19°C (day/night) and 60% rh in a greenhouse. The percentage leaf area covered by disease was assessed when an appropriate level of disease appeared on untreated check plants (11 - 13 days after application). Uncinula necator on grape with a preventative application.
  • test plants were inoculated by shaking plants infected with grape powdery mildew above them 1 day after application.
  • the inoculated test plants were incubated at 24/22 °C (day/night) and 70% rh under a light regime of 14/10 h (light/dark) and the percentage leaf area covered by disease was assessed when an appropriate level of disease appeared on untreated check plants (7 - 9 days after application).

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne un procédé pour combattre ou prévenir des champignons phytopathogènes appartenant à Glomerella lagenarium, Magnaporthe grisea, Mycosphaerella arachidis, Phaeosphaeria nodorum, Pyrenophora teres, Venturia equalis, Botrytis eliptica, Botrytis tulipae, Corynespora cassiicola, Cercospora kikuchii, et/ou Cercospora sojina, sur une plante ou sur un matériel de propagation de plantes de celle-ci, le procédé consistant à appliquer sur la plante, un matériel de propagation de plantes ou un locus de celle-ci, une composition contenant de la jawsamycine.
PCT/EP2024/080247 2023-10-26 2024-10-25 Procédé de lutte contre des champignons phytopathogènes à l'aide de jawsamycine Pending WO2025088131A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP23205996 2023-10-26
EP23205996.4 2023-10-26

Publications (1)

Publication Number Publication Date
WO2025088131A1 true WO2025088131A1 (fr) 2025-05-01

Family

ID=88558531

Family Applications (4)

Application Number Title Priority Date Filing Date
PCT/EP2024/080248 Pending WO2025088132A1 (fr) 2023-10-26 2024-10-25 Procédé de lutte contre des champignons phytopathogènes
PCT/EP2024/080247 Pending WO2025088131A1 (fr) 2023-10-26 2024-10-25 Procédé de lutte contre des champignons phytopathogènes à l'aide de jawsamycine
PCT/EP2024/080245 Pending WO2025088129A1 (fr) 2023-10-26 2024-10-25 Procédé de lutte contre des champignons phytopathogènes
PCT/EP2024/080246 Pending WO2025088130A1 (fr) 2023-10-26 2024-10-25 Procédé de lutte contre des champignons phytopathogènes

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/EP2024/080248 Pending WO2025088132A1 (fr) 2023-10-26 2024-10-25 Procédé de lutte contre des champignons phytopathogènes

Family Applications After (2)

Application Number Title Priority Date Filing Date
PCT/EP2024/080245 Pending WO2025088129A1 (fr) 2023-10-26 2024-10-25 Procédé de lutte contre des champignons phytopathogènes
PCT/EP2024/080246 Pending WO2025088130A1 (fr) 2023-10-26 2024-10-25 Procédé de lutte contre des champignons phytopathogènes

Country Status (1)

Country Link
WO (4) WO2025088132A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02101006A (ja) 1988-10-07 1990-04-12 Fujisawa Pharmaceut Co Ltd 植物用殺菌剤
WO2000013487A2 (fr) * 1998-09-09 2000-03-16 Green Care Laboratories Ltd. Composes d'inhibition de la croissance de champignons vegetaux, leur procede de preparation, compositions les contenant et leur utilisation dans un procede de lutte contre les champignons vegetaux
US20230081771A1 (en) * 2020-02-11 2023-03-16 Syngenta Crop Protection Ag Method of controlling fungi

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8708635D0 (en) 1987-04-10 1987-05-13 Fujisawa Pharmaceutical Co Fr-900848 substance
CA1340685C (fr) 1988-07-29 1999-07-27 Frederick Meins Sequences d'adn codant des polypeptides possedant une activite de beta-1,3-glucanase
EP0392225B1 (fr) 1989-03-24 2003-05-28 Syngenta Participations AG Plantes transgéniques résistantes aux maladies
US5639949A (en) 1990-08-20 1997-06-17 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
AR114634A1 (es) 2018-02-21 2020-09-30 Syngenta Crop Protection Ag Método y dispositivo de siembra de precisión

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02101006A (ja) 1988-10-07 1990-04-12 Fujisawa Pharmaceut Co Ltd 植物用殺菌剤
WO2000013487A2 (fr) * 1998-09-09 2000-03-16 Green Care Laboratories Ltd. Composes d'inhibition de la croissance de champignons vegetaux, leur procede de preparation, compositions les contenant et leur utilisation dans un procede de lutte contre les champignons vegetaux
US20230081771A1 (en) * 2020-02-11 2023-03-16 Syngenta Crop Protection Ag Method of controlling fungi

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
A. BARRETT ET AL., J. AM. CHEM. SOC., vol. 118, no. 45, 1996, pages 11030 - 11037
FU, NATURE COMMUNICATIONS, 2020, pages 11 - 3387
FU, Y. ET AL., NAT COMMUN, vol. 11, 2020, pages 3387
MASARU ET AL., THE JOURNAL OF ANTIBIOTICS, vol. 43, 1990, pages 748 - 754
MICROBIAL GENETICS BULLETIN, vol. 13, 1956, pages 42 - 43
YOSHIDA ET AL., J. OF ANTIBIOTICS, 1990, pages 748 - 754

Also Published As

Publication number Publication date
WO2025088129A1 (fr) 2025-05-01
WO2025088132A1 (fr) 2025-05-01
WO2025088130A1 (fr) 2025-05-01

Similar Documents

Publication Publication Date Title
RU2444896C2 (ru) Способы и композиция для воздействия на рост и для борьбы с болезнями
JP6758296B2 (ja) 植物の成長に利益をもたらす及び植物病害を治療するための微生物組成物
JP5443176B2 (ja) 組み合わせ殺病害虫剤
JP2018502111A (ja) バチルス・アミロリケファシエンス（ｂａｃｉｌｌｕｓ ａｍｙｌｏｌｉｑｕｅｆａｃｉｅｎｓ）ｒｔｉ３０１組成物および植物成長に利益を与えかつ植物病害を処置するための使用方法
CN101686679A (zh) 杀线虫剂组合物及其使用方法
JP2010520865A5 (fr)
JPH08503223A (ja) 相乗性殺微生物剤組成物
TW200911119A (en) Fungicidal active compound combination
UA126823C2 (uk) Способи контролю або попередження зараження овочевої рослини, рослин томата та картоплі фітопатогенними мікроорганізмами
CN108935486A (zh) 一种杀菌组合物
WO2015135422A1 (fr) Procédé de protection de plante contre une maladie de plante
WO2010058830A1 (fr) Procédé permettant de lutter contre les maladies des plantes agricoles et horticoles
JP2019026616A (ja) ベンゾオキサジノン化合物を有効成分とする農園芸用殺菌剤
EP3367797A1 (fr) Utilisation antifongique d'ilicicoline h en agriculture
WO2025088131A1 (fr) Procédé de lutte contre des champignons phytopathogènes à l'aide de jawsamycine
EP4199721B1 (fr) Procédés de lutte ou de prévention d'infestation de plantes par des nématodes parasites des plantes du genre aphelenchoides spp., en particulier aphelenchoides besseyi
WO2003011029A1 (fr) Composition permettant de lutter contre une bacterie pathogene de plante et procede de lutte contre ladite bacterie pathogene de plante
JP2025534468A (ja) 殺真菌性混合物
JPS6368505A (ja) 農業用殺虫殺菌組成物
US20240057600A1 (en) Methods for controlling or preventing infestation of plants by a phytopathogenic microorganism of the genus aspergillus
CN107593710B (zh) 一种杀菌组合物
WO2021063818A1 (fr) Procédés de lutte ou de prévention de l'infestation de plants de banane par des micro-organismes phytopathogènes du genre pseudocercospora
JP2007045818A (ja) 農園芸用殺ダニ剤組成物
JPH0784363B2 (ja) 農業用の殺虫殺菌組成物
JPH072607B2 (ja) 農業用殺虫殺菌組成物

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24794823

Country of ref document: EP

Kind code of ref document: A1