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WO2025087247A1 - Nouvel appareil d'électrophorèse verticale et son utilisation - Google Patents

Nouvel appareil d'électrophorèse verticale et son utilisation Download PDF

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Publication number
WO2025087247A1
WO2025087247A1 PCT/CN2024/126587 CN2024126587W WO2025087247A1 WO 2025087247 A1 WO2025087247 A1 WO 2025087247A1 CN 2024126587 W CN2024126587 W CN 2024126587W WO 2025087247 A1 WO2025087247 A1 WO 2025087247A1
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WO
WIPO (PCT)
Prior art keywords
electrophoresis
gel
plate
electrode
detachable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/CN2024/126587
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English (en)
Chinese (zh)
Inventor
望超
陈鑫
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Jinsirui Science and Technology Biology Corp
Original Assignee
Nanjing Jinsirui Science and Technology Biology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing Jinsirui Science and Technology Biology Corp filed Critical Nanjing Jinsirui Science and Technology Biology Corp
Publication of WO2025087247A1 publication Critical patent/WO2025087247A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/453—Cells therefor

Definitions

  • the present application relates to a vertical electrophoresis device, which is compatible with the use of more than two gel blocks of different sizes, and in particular, is compatible with the use of mini gel and midi gel.
  • Electrophoresis is a commonly used bioanalytical technique that uses the differences in migration rates and isoelectric points of nucleic acids or proteins in an electric field to achieve molecular separation, identification, and quantification.
  • a disk electrophoresis device which includes two upper and lower electrophoresis tanks and a cover with platinum electrodes.
  • the upper tank has several holes, which are sealed with silicone plugs to insert electrophoresis gel tubes, and unused holes are plugged with silicone rubber plugs. Due to differences in the preparation process and later losses, there will be large inner diameter deviations between the holes, which makes it difficult to keep the upper electrophoresis tank sealed. Therefore, disk electrophoresis has been almost replaced by plate electrophoresis (horizontal/vertical) and is only used in small quantities in the analysis of large protein complexes;
  • Horizontal electrophoresis device which generally includes a horizontal electrophoresis tank base, a cooling plate and electrodes.
  • the positive and negative electrodes are located at the two ends of the horizontal electrophoresis tank base, and the separation gel is bridged by filter paper or agarose gel.
  • Horizontal electrophoresis is widely used in agarose nucleic acid electrophoresis, but in protein electrophoresis, due to the need to use discontinuous gels, vertical electrophoresis is almost entirely used. Only in isoelectric focusing electrophoresis can horizontal electrophoresis be used;
  • Vertical electrophoresis device its basic structure and electrophoresis principle are similar to those of disk electrophoresis, the main difference is that the gel preparation and electrophoresis are not in the electrophoresis tube, but between two vertically placed parallel glass plates.
  • Vertical electrophoresis device can be used for protein electrophoresis, as well as nucleic acid electrophoresis.
  • vertical electrophoresis is the most commonly used and widely applicable type of electrophoresis.
  • a vertical electrophoresis device for electrophoresis, it is necessary to prepare gel plates, electrophoresis buffer, a DC power supply, and sample loading equipment, etc.
  • the assembly of the electrophoresis device usually includes removing the comb on the upper part of the gel plate, removing the sealing tape on the bottom and/or side of the gel plate when necessary, inserting the gel plate vertically into the gap in the inner core of the electrophoresis tank, and sealing to form an inner electrophoresis tank, ensuring that the short gel plate faces the inner electrophoresis tank and the long gel plate faces the outer electrophoresis tank, wherein the negative electrode is usually connected in the inner tank and the positive electrode is usually connected in the outer tank. Add electrophoresis buffer to the inner tank and outer tank respectively.
  • the liquid level in the outer tank must be lower than the liquid level in the inner tank to avoid short circuits, and the liquid level in the inner tank must be higher than the short plate of the gel plate to ensure that the inner tank buffer is in full contact with the gel on the upper part of the gel plate (comb hole position) to avoid short circuits. Then, use a sampler to load the sample according to actual needs. Set a constant voltage or current to separate nucleic acid or protein molecules.
  • the inventor of the present application has designed a new vertical electrophoresis device, which may include an electrophoresis tank and a detachable electrophoresis core, wherein the electrophoresis tank is provided with a first electrode (for example, a positive electrode) and a first electrode plug connected thereto, as well as a second electrode plug and an electrode plug guide connected thereto, and the detachable electrophoresis core is provided with a second electrode (for example, a negative electrode), and the second electrode can be electrically connected to the second electrode plug through the electrode plug guide.
  • a first electrode for example, a positive electrode
  • a first electrode plug for example, a first electrode plug connected thereto
  • the detachable electrophoresis core is provided with a second electrode (for example, a negative electrode)
  • the second electrode can be electrically connected to the second electrode plug through the electrode plug guide.
  • the vertical electrophoresis device of the present application can i) reduce the collision damage to the electrode during the assembly of the detachable electrophoresis core by assembling one electrode (for example, the positive electrode) in the electrophoresis tank; ii) arrange the electrode endpoints according to the type of the detachable electrophoresis core (a core that can only assemble a single gel plate, such as a midi-type core, or a core that can assemble two gel plates, such as a mini-type core), so that the endpoints are electrically connected to the electrode plug set in the electrophoresis tank through the electrode plug guide, thereby minimizing the size of the electrophoresis tank while assembling different types of electrophoresis cores, reducing the preparation of electrode plugs and reducing production costs; iii) arrange the two endpoints of the electrode on the detachable electrophoresis core in mirror symmetry Or arranged in a centrally symmetrical manner to facilitate "error-proof" assembly of the
  • a vertical electrophoresis device which may include:
  • an electrophoresis tank which may include a tank base and an upper cover, wherein the tank base may include a first electrode, a first electrode plug, a second electrode plug, and an electrode plug guide, and
  • each set of detachable electrophoresis cores may include more than one detachable electrophoresis core
  • each detachable electrophoresis core may include a core frame and a second electrode, and the core frame may be configured to assemble a gel plate or a gel replacement plate
  • the first electrode can be configured to be connected to the first electrode plug and can be electrically connected to the first electrode plug,
  • the second electrode may be configured to be electrically connected to the electrode plug guide, and the electrode plug guide may be configured to be connected to the second electrode plug and be electrically connected to the second electrode plug.
  • the detachable electrophoresis core can be configured to be fixed vertically in the electrophoresis tank.
  • the electrophoresis tank may also contain electrical cables and a power supply for the electrophoresis apparatus.
  • the upper cover can be configured to cover the tank base, in particular to cover the tank base in a sealed manner.
  • the upper cover can include a first power interface and a second power interface.
  • the first power interface can be configured to be electrically connected to the first electrode plug and can be electrically connected to the cable.
  • the second power interface can be configured to be electrically connected to the second electrode plug and can be electrically connected to the cable.
  • the inner core frame of each group of detachable electrophoresis inner cores can be configured to form a sealed slot body with an upper opening together with the gel plate and/or the gel replacement plate.
  • the inner core frame of each detachable electrophoresis inner core may include a frame body that is substantially in the shape of a rectangular parallelepiped.
  • the inner core frame may further include a sealing strip and a groove for accommodating the sealing strip, which is configured to allow the gel plate or gel replacement plate to be in sealing contact with the inner core frame to form a sealing groove body with an upper opening.
  • the inner core frame may further include a gel sheet or gel replacement sheet fastener configured to compress the gel sheet or gel replacement sheet toward the inner core frame.
  • the tank base can be configured to accommodate a detachable electrophoresis inner core selected from any group.
  • the tank base can be configured to only accommodate a detachable electrophoresis inner core selected from any group.
  • the vertical electrophoresis device may include more than two groups of detachable electrophoresis cores, wherein each group of detachable electrophoresis cores can be equipped with gel plates or gel replacement plates of different sizes.
  • the vertical electrophoresis device may include more than two groups of detachable electrophoresis cores, and each group of detachable electrophoresis cores can be equipped with gel plates or gel replacement plates of different sizes.
  • Each detachable electrophoresis core in each group can be configured to assemble two gel plates, or one gel plate and one gel replacement plate.
  • the vertical electrophoresis device may include more than two groups of detachable electrophoresis cores, each group of detachable electrophoresis cores can be equipped with gel plates or gel replacement plates of different sizes, wherein each detachable electrophoresis core in each group can be configured to only be able to assemble one gel plate.
  • the vertical electrophoresis device may include more than two groups of detachable electrophoresis cores, wherein each detachable electrophoresis core of one or more groups of detachable electrophoresis cores may be configured to assemble two gel plates, or one gel plate and one gel replacement plate, and each of the one or more groups of detachable electrophoresis cores may be configured to be able to assemble only one gel plate.
  • the size of the gel plate or gel substitute plate that can be assembled with each detachable electrophoresis inner core can be about 10cm ⁇ 10cm, or about 10cm ⁇ 15cm.
  • the vertical electrophoresis device can include two sets of detachable electrophoresis inner cores, wherein the size of the gel plate or gel substitute plate that can be assembled with one set of detachable electrophoresis inner cores can be about 10cm ⁇ 10cm, and the size of the gel plate or gel substitute plate that can be assembled with the other set of detachable electrophoresis inner cores can be about 10cm ⁇ 15cm.
  • the trough base may be substantially in the shape of a cuboid, comprising a bottom plate and four side plates.
  • the first electrode plug and the second electrode plug may be respectively disposed in a groove base.
  • the electrode plug guide can be arranged on the top of the same side plate or the side plate adjacent to the side plate close to the second electrode plug and protrude into the inside of the slot base.
  • the electrode plug guide can protrude into the inside of the slot base in the horizontal direction.
  • the electrode plug guide can protrude into the inside of the slot base in the horizontal direction in a direction parallel to the side plate on which the first electrode plug and the second electrode plug are arranged.
  • a detachable electrophoresis core configured to be able to assemble two gel plates, or a gel plate and a gel replacement plate, can be configured so that one end of the second electrode extends outward from a side plate adjacent to the gel plate to be assembled in the horizontal direction on the top surface of the frame body of the core frame in a direction perpendicular to the side plate and extends after bending, presenting a " ⁇ " shape, or extends outward and extends in both directions after bending, presenting a "T" shape.
  • the two ends of the second electrode can be extended outward from two side plates adjacent to the gel plate to be assembled in a horizontal direction on the top surface of the frame body in a centrally symmetrical manner relative to the center of the top surface of the frame body in a direction perpendicular to the two side plates and extended after bending, presenting a " ⁇ " shape, or extended outward and extended in two directions after bending, presenting a "T" shape.
  • one end of the second electrode can be extended outward from the side plate opposite to the gel plate to be assembled in a horizontal direction on the top surface of the frame body.
  • one end of the second electrode can be extended outward from the side plate opposite to the gel plate to be assembled in a direction parallel to the side plate in a horizontal direction on the top surface of the frame body.
  • the two ends of the second electrode can be extended outward from the side plate opposite to the gel plate to be assembled in a mirror-symmetrical manner relative to a plane passing through the midpoint of the top edge of the gel plate to be assembled and perpendicular to the gel plate to be assembled on the top surface of the frame body in a horizontal direction.
  • the two ends of the second electrode can be extended outward from the side plate opposite to the gel plate to be assembled in a mirror-symmetrical manner relative to a plane passing through the midpoint of the top edge of the gel plate to be assembled and perpendicular to the gel plate on the top surface of the frame body in a horizontal direction in a direction parallel to the side plate.
  • the first electrode plug and the second electrode plug may be arranged at the top of the two side plates including the short sides of the tank base in a mirror-symmetrical manner relative to the connecting line of the centers of the two long sides of the tank base, and the electrode plug guide may be arranged at the top of the side plate where the second electrode plug is arranged and protrude into the tank base.
  • the electrode plug guide may protrude into the tank base in the horizontal direction.
  • the electrode plug guide may protrude into the tank base in the horizontal direction in a direction perpendicular to the side plate where it is located.
  • the first electrode plug and the second electrode plug may be arranged at the basic center of the top of the two side plates including the short sides of the tank base, respectively, and the electrode plug guide may be arranged at the top of the side plate where the second electrode plug is arranged and protrude into the tank base.
  • the electrode plug guide may be arranged at the basic center of the top of the side plate where the second electrode plug is arranged and protrude into the tank base.
  • the electrode plug guide may be arranged at the basic center of the top of the side plate where the second electrode plug is arranged and protrude into the tank base.
  • the electrode plug guide may be arranged at the basic center of the top of the side plate where the second electrode plug is arranged and protrude into the tank base in the horizontal direction.
  • the electrode plug guide can be arranged at the substantially center of the top of the side plate where the second electrode plug is arranged and protrude in the horizontal direction in a direction perpendicular to the side plate where it is located toward the inside of the tank base.
  • Each detachable electrophoresis core includes a configuration capable of assembling two gel plates or one gel plate.
  • the detachable electrophoresis core configured to be equipped with a gel replacement plate and a detachable electrophoresis core configured to be equipped with only one gel plate can extend one end of the second electrode outwardly from a side plate adjacent to the gel plate to be assembled in a horizontal direction on the top surface of the frame body in a direction parallel to the gel plate to be assembled.
  • each detachable electrophoresis core including a detachable electrophoresis core configured to be equipped with two gel plates, or a gel plate and a gel replacement plate, and a detachable electrophoresis core configured to be equipped with only one gel plate, can extend the two ends of the second electrode outwardly from two side plates adjacent to the gel plate to be assembled in a horizontal direction on the top surface of the frame body in a direction parallel to the gel plate to be assembled in a centrally symmetrical manner relative to the center of the top surface of the frame body.
  • the core frame may further include a first protrusion and a second protrusion extending outwardly from two side plates of the frame body in a horizontal direction in a direction parallel to the gel plate to be assembled on the top surface of the frame body.
  • One end of the second electrode extending outward from the side plate may be arranged on the first protrusion and configured to protrude outward from the first protrusion.
  • the two ends of the second electrode may be arranged on the first protrusion and the second protrusion, respectively, and configured to protrude outward from the first protrusion and the second protrusion.
  • the core frame further includes a third protrusion and a fourth protrusion extending outwardly from two side plates of the frame body adjacent to the gel plate to be assembled in a horizontal direction in a direction parallel to the gel plate to be assembled on the top surface of the frame body.
  • the length of the sides of the third protrusion and the fourth protrusion connected to the two side plates may be less than the length of the sides of the two side plates connected to the third protrusion and the fourth protrusion.
  • the detachable electrophoresis core configured to be able to assemble two gel plates, or one gel plate and one gel replacement plate, the thickness of the bottom plate of the frame body and/or the two side plates can be relatively large, for example, each can be 1.0 cm, 1.5 cm, 2.0 cm, or 2.5 cm. In some embodiments, the thickness of the bottom plate and the two side plates of the frame body can be relatively large, for example, each can be 1.0 cm, 1.5 cm, 2.0 cm, or 2.5 cm.
  • the first electrode can be located at any position of the tank base, configured to be connected to the first electrode plug, and at least a portion of it contacts the electrophoresis buffer when the electrophoresis buffer is poured into the tank base.
  • the first electrode can be arranged on a side plate on which the first electrode plug is arranged.
  • the first electrode can be arranged on a side plate and a bottom plate on which the first electrode plug is arranged.
  • the first electrode can be attached to the surface of the tank base or embedded in the tank base.
  • the first electrode can be embedded in the tank base or attached to the surface of the tank base by integral molding, such as injection molding.
  • the second electrode except for one or two ends having the above-mentioned special configuration, the rest of the parts can be located at any position of the inner core frame, and is configured so that at least a part of it contacts with the electrophoresis buffer when the electrophoresis buffer is poured into the sealed groove formed by the inner core frame and the gel plate to be assembled.
  • the second electrode can be attached to the surface of the inner core frame, or embedded in the inner core frame.
  • the second electrode can be formed together with the inner core frame in an integral manner, such as by injection molding.
  • the second electrode can be made in an integral manner, such as by injection molding, in the form of being embedded in the inner core frame.
  • the second electrode in contact with the electrophoresis buffer The part can be attached to or embedded in the bottom plate of the frame body, or attached to or embedded in the side plate of the frame body opposite to the gel plate to be assembled, or attached to or embedded in the bottom plate of the frame body and the side plate opposite to the gel plate to be assembled.
  • the part of the second electrode in contact with the electrophoresis buffer can be attached to or embedded in the side plate of the frame body opposite to the gel plate to be assembled below the height of the short plate of the gel plate to be assembled.
  • the part of the second electrode in contact with the electrophoresis buffer can be attached to or embedded in the side plate of the frame body opposite to the gel plate to be assembled at a height slightly lower than the short plate of the gel plate to be assembled.
  • the part of the second electrode in contact with the electrophoresis buffer can be attached to or embedded in one or two side plates of the frame body adjacent to the gel plate to be assembled below the height of the short plate of the gel plate to be assembled.
  • the portion of the second electrode in contact with the electrophoresis buffer may be attached to or embedded in the bottom plate of the frame body and one or two side plates adjacent to the gel plate to be assembled below the height of the short plate of the gel plate to be assembled.
  • the portion of the second electrode in contact with the electrophoresis buffer may be attached to or embedded in the two side plates of the frame body adjacent to the gel plate to be assembled below the height of the short plate of the gel plate to be assembled, and spans the frame body, wherein the width of the portion of the second electrode in contact with the electrophoresis buffer is less than the width of the one or two side plates of the frame body adjacent to the gel plate to be assembled.
  • the portion of the second electrode in contact with the electrophoresis buffer may be attached to or embedded in the two side plates of the frame body adjacent to the gel plate to be assembled at the height of the short plate of the gel plate to be assembled, and spans the frame body, wherein the width of the portion of the second electrode in contact with the electrophoresis buffer is less than the width of the one or two side plates of the frame body adjacent to the gel plate to be assembled.
  • the inner core frame may further include an electrophoresis inner core fixing member configured to vertically fix the detachable electrophoresis inner core in the tank base of the electrophoresis tank.
  • the electrophoresis inner core fixing member may be a first protrusion and a second protrusion. In some embodiments, the electrophoresis inner core fixing member may be a third protrusion and a fourth protrusion.
  • the tank base may also include a removable electrophoresis core fixture, which may be configured to vertically fix the removable electrophoresis core in the tank base of the electrophoresis tank.
  • the removable electrophoresis core fixture may match the electrophoresis core fixture on the removable electrophoresis core to vertically fix the removable electrophoresis core in the tank base of the electrophoresis tank.
  • the tank base may include a groove on each of the two opposite side panels including the short side of the tank base, configured to match the first protrusion and the second protrusion, or the third protrusion and the fourth protrusion, to vertically fix the removable electrophoresis core to the electrophoresis tank.
  • the first power interface may be a positive power interface
  • the second power interface may be a negative power interface
  • the first electrode may be a positive electrode
  • the second electrode may be a negative electrode.
  • the positive electrode and the negative electrode may be made of any suitable conductive material, such as a conductive metal, a conductive alloy, graphite, etc., including, but not limited to, consumable electrode materials (such as copper), inert materials (such as platinum, titanium, graphite), electrode materials with inert metals (such as platinum, iridium) on the surface, stainless steel, etc.
  • the positive electrode may be made of a metal (stainless steel, copper, iron, titanium, or an alloy thereof) with an inert conductive metal (such as gold, platinum, iridium, tantalum) on the surface.
  • the positive electrode may be made of a stainless steel material with an inert conductive metal (such as gold, platinum, iridium, tantalum) on the surface.
  • the negative electrode may be made of a stainless steel material. The stainless steel material may be selected from 204, 304, 314 or 316 stainless steel.
  • the first electrode plug and the second electrode plug can be made of any suitable conductive material, such as conductive metal, conductive alloy, graphite, etc., including, but not limited to, consumable electrode materials (such as copper), inert materials (such as platinum, titanium, graphite), stainless steel, etc.
  • conductive metal such as conductive metal, conductive alloy, graphite, etc.
  • consumable electrode materials such as copper
  • inert materials such as platinum, titanium, graphite
  • stainless steel etc.
  • the electrode plug guide can be made of any suitable conductive material, such as conductive metal, conductive alloy, graphite, etc., including, but not limited to, consumable electrode materials (such as copper), inert materials (such as platinum, titanium, graphite), stainless steel, etc.
  • conductive metal such as conductive metal, conductive alloy, graphite, etc.
  • consumable electrode materials such as copper
  • inert materials such as platinum, titanium, graphite
  • stainless steel etc.
  • a vertical electrophoresis device which may include:
  • an electrophoresis tank which may include a tank base and an upper cover, wherein the tank base may include a first electrode, a first electrode plug, a second electrode plug, and an electrode plug guide, and
  • each set of detachable electrophoresis cores may include more than one detachable electrophoresis core
  • each detachable electrophoresis core may include a core frame and a second electrode, and the core frame may be configured to assemble a gel plate or a gel replacement plate
  • the first electrode can be configured to be connected to the first electrode plug and can be electrically connected to the first electrode plug,
  • the second electrode can be configured to be electrically connected to the electrode plug guide, and the electrode plug guide can be configured to be connected to the second electrode plug and can be electrically connected to the second electrode plug.
  • the trough base may include a first trough body and a second trough body separated from each other.
  • the first trough body and the second trough body may both be substantially rectangular parallelepipeds.
  • the first trough body and the second trough body may be arranged with their long sides facing each other and their short sides in a line, and may share a side plate.
  • the first electrode plug and the second electrode plug may be respectively disposed at two top ends of a side plate shared by the first tank body and the second tank body.
  • the electrode plug guide can be arranged on the top of the common side plate or the side plate adjacent to the side plate and close to the second electrode plug and protrude into the first slot body and the second slot body.
  • the electrode plug guide can protrude into the first slot body and the second slot body in a horizontal direction.
  • the electrode plug guide can protrude into the first slot body and the second slot body in a horizontal direction in a direction parallel to the common side plate.
  • the electrode plug guide can, for example, be U-shaped, wherein the bottom of the U is connected to the second electrode plug.
  • the first tank body and the second tank body can be configured to each accommodate a detachable electrophoresis inner core selected from any group.
  • the first tank body and the second tank body can be configured to each accommodate only one detachable electrophoresis inner core selected from any group.
  • the inner core frame of each group of detachable electrophoresis inner cores can be configured to form a sealed slot body with an upper opening together with the gel plate and/or the gel replacement plate.
  • the inner core frame may further include a sealing strip and a groove for accommodating the sealing strip, which is configured to allow the gel plate or gel replacement plate to be in sealing contact with the inner core frame to form a sealing groove body with an upper opening.
  • the inner core frame may also include a gel sheet or gel replacement sheet fastener configured to press the gel against the inner core frame.
  • Gel sheet or gel substitute sheet configured to press the gel against the inner core frame.
  • the vertical electrophoresis apparatus may comprise:
  • each detachable electrophoresis core in each set of detachable electrophoresis cores can be configured to assemble two gel plates, or one gel plate and one gel replacement plate;
  • each detachable electrophoresis core in each set can be configured to fit only one gel plate
  • each detachable electrophoresis core in one or more groups of detachable electrophoresis cores is configured to be able to assemble two gel plates, or one gel plate and one gel replacement plate, and each detachable electrophoresis core in one or more groups of detachable electrophoresis cores is configured to be able to assemble only one gel plate.
  • the vertical electrophoresis device can include more than two groups of detachable electrophoresis cores, wherein the sizes of gel plates or gel replacement plates that can be assembled in each group of detachable electrophoresis cores can be different.
  • the size of the gel plate or gel substitute plate that can be assembled with each detachable electrophoresis inner core can be about 10cm ⁇ 10cm, or about 10cm ⁇ 15cm.
  • the vertical electrophoresis device can include two sets of detachable electrophoresis inner cores, wherein the size of the gel plate or gel substitute plate that can be assembled with one set of detachable electrophoresis inner cores can be about 10cm ⁇ 10cm, and the size of the gel plate or gel substitute plate that can be assembled with the other set of detachable electrophoresis inner cores can be about 10cm ⁇ 15cm.
  • the inner core frame of each detachable electrophoresis inner core may include a frame body that is substantially in the shape of a rectangular parallelepiped.
  • one end of the second electrode can be configured to extend outward from a side plate adjacent to the gel plate to be assembled in a horizontal direction on the top surface of the frame body of the core frame in a direction perpendicular to the side plate and extend after bending, presenting a " ⁇ " shape, or extend outward and extend in two directions after bending, presenting a "T" shape.
  • the two ends of the second electrode can be respectively extended outward from two side plates adjacent to the gel plate to be assembled in a direction perpendicular to the two side plates on the top surface of the frame body in a centrally symmetrical manner relative to the center of the top surface of the frame body in a horizontal direction on the top surface of the frame body and extend after bending, presenting a " ⁇ " shape, or extend outward and extend in two directions after bending, presenting a "T" shape.
  • the two ends of the second electrode can be extended outward from the side plate opposite to the gel plate to be assembled in a mirror-symmetrical manner relative to a plane passing through the midpoint of the top edge of the gel plate to be assembled and perpendicular to the gel plate to be assembled on the top surface of the frame body in a horizontal direction.
  • the two ends of the second electrode can be extended outward from the side plate opposite to the gel plate to be assembled in a mirror-symmetrical manner relative to a plane passing through the midpoint of the top edge of the gel plate to be assembled and perpendicular to the gel plate to be assembled on the top surface of the frame body in a horizontal direction in a direction parallel to the side plate.
  • the electrophoresis tank may also contain electrical cables and a power supply for the electrophoresis apparatus.
  • the upper cover can be configured to cover the tank base, in particular to cover the tank base tightly.
  • the upper cover can include a first
  • the first power interface can be configured to be electrically connected to the first electrode plug and to be electrically connected to the cable.
  • the second power interface can be configured to be electrically connected to the second electrode plug and to be electrically connected to the cable.
  • the core frame may further include a first protrusion and a second protrusion extending outwardly from two side plates of the frame body in a horizontal direction in a direction parallel to the gel plate to be assembled on the top surface of the frame body.
  • One end of the second electrode extending outward from the side plate may be arranged on the first protrusion and configured to protrude outward from the first protrusion.
  • the two ends of the second electrode are respectively arranged on the first protrusion and the second protrusion, and configured to protrude outward from the first protrusion and the second protrusion.
  • the core frame further includes a third protrusion and a fourth protrusion extending outwardly from two side plates of the frame body adjacent to the gel plate in a horizontal direction in a direction parallel to the gel plate on the top surface of the frame body.
  • the length of the sides of the third protrusion and the fourth protrusion connected to the two side plates may be less than the length of the sides of the two side plates connected to the third protrusion and the fourth protrusion.
  • the detachable electrophoresis core configured to be able to assemble two gel plates, or one gel plate and one gel replacement plate, the thickness of the bottom plate of the frame body and/or the two side plates can be relatively large, for example, each can be 1.0 cm, 1.5 cm, 2.0 cm, or 2.5 cm. In some embodiments, the thickness of the bottom plate and the two side plates of the frame body can be relatively large, for example, each can be 1.0 cm, 1.5 cm, 2.0 cm, or 2.5 cm.
  • the first electrode can be arranged at any position of the tank base, configured to be connected to the first electrode plug, at least a portion of which is in contact with the electrophoresis buffer to be added in the first tank body, and at least a portion of which is in contact with the electrophoresis buffer to be added in the second tank body.
  • the first electrode can be arranged on a common side plate of the first tank body and the second tank body.
  • the first electrode can be arranged on a common side plate of the first tank body and the second tank body, and on the bottom plate of the first tank body and/or the second tank body.
  • the first electrode can be attached to the surface of the first tank body, or embedded in the first tank body, and can be attached to the surface of the second tank body, or embedded in the second tank body.
  • the first electrode can be embedded in the first tank body and/or the second tank body, or attached to the surface of the first tank body and/or the second tank body by integral molding, such as injection molding.
  • the second electrode except for one or two ends having the above-mentioned special configuration, the other parts can be located at any position of the inner core frame, and is configured so that at least a part of it contacts the electrophoresis buffer when the electrophoresis buffer is poured into the sealed groove formed by the inner core frame and the gel plate to be assembled.
  • the second electrode can be attached to the surface of the inner core frame or embedded in the inner core frame.
  • the second electrode can be formed together with the inner core frame in an integral manner, such as by injection molding.
  • the second electrode can be made in the form of being embedded in the inner core frame by an integral manner, such as injection molding.
  • the part of the second electrode that contacts the electrophoresis buffer can be attached to or embedded in the bottom plate of the frame body, or attached to or embedded in the side plate of the frame body opposite to the gel plate to be assembled, or attached to or embedded in the bottom plate of the frame body and the side plate opposite to the gel plate to be assembled.
  • the portion of the second electrode in contact with the electrophoresis buffer can be attached to or embedded in the side plate of the frame body opposite to the gel plate to be assembled, below the height of the short plate of the gel plate to be assembled.
  • the portion of the second electrode in contact with the electrophoresis buffer can be attached to or embedded in the side plate of the frame body opposite to the gel plate to be assembled at a height slightly lower than the short plate of the gel plate to be assembled.
  • the portion of the second electrode in contact with the electrophoresis buffer can be attached to or embedded in one or two side plates of the frame body adjacent to the gel plate to be assembled, below the height of the short plate of the gel plate to be assembled.
  • the portion of the second electrode in contact with the electrophoresis buffer can be attached to or embedded in the bottom plate of the frame body and one or two side plates adjacent to the gel plate to be assembled, below the height of the short plate of the gel plate to be assembled.
  • the portion of the second electrode in contact with the electrophoresis buffer may be attached to or embedded in two side plates of the frame body adjacent to the gel plate to be assembled below the height of the short plate of the gel plate to be assembled, and spans the frame body, wherein the width of the portion of the second electrode in contact with the electrophoresis buffer is less than the width of one or two side plates of the frame body adjacent to the gel plate to be assembled.
  • the portion of the second electrode in contact with the electrophoresis buffer may be attached to or embedded in two side plates of the frame body adjacent to the gel plate to be assembled at the height of the short plate of the gel plate to be assembled, and spans the frame body, wherein the width of the portion of the second electrode in contact with the electrophoresis buffer is less than the width of one or two side plates of the frame body adjacent to the gel plate to be assembled.
  • the inner core frame may further include an electrophoresis inner core fixing member configured to vertically fix the detachable electrophoresis inner core in the tank base of the electrophoresis tank.
  • the electrophoresis inner core fixing member may be a first protrusion and a second protrusion. In some embodiments, the electrophoresis inner core fixing member may be a third protrusion and a fourth protrusion.
  • the tank base may include a groove on two opposite side plates of the second tank body containing short sides, respectively, configured to match the first protrusion and the second protrusion, or the third protrusion and the fourth protrusion, to vertically fix the removable electrophoresis core to the electrophoresis tank.
  • the tank base may include a groove on two opposite side panels of the first tank body and the second tank body including their respective short sides, respectively, configured to match the first protrusion and the second protrusion, and/or the third protrusion and the fourth protrusion to vertically fix the removable electrophoresis core to the electrophoresis tank.
  • the first power interface may be a positive power interface
  • the second power interface may be a negative power interface
  • the first electrode may be a positive electrode
  • the second electrode may be a negative electrode.
  • the positive electrode and the negative electrode may be made of any suitable conductive material, such as conductive metal, conductive alloy, graphite, etc., including, but not limited to, consumable electrode materials (such as copper), inert materials (such as platinum, titanium, graphite), and surfaces with inert metals (such as platinum, iridium). Electrode material, stainless steel, etc.
  • the positive electrode can be made of a metal (stainless steel, copper, iron, titanium or its alloy) with an inert conductive metal (e.g., gold, platinum, iridium, tantalum) on the surface.
  • the positive electrode can be made of a stainless steel material with an inert conductive metal (e.g., gold, platinum, iridium, tantalum) on the surface.
  • the negative electrode can be made of a stainless steel material. The stainless steel material can be selected from 204, 304, 314 or 316 stainless steel.
  • the first electrode plug and the second electrode plug can be made of any suitable conductive material, such as conductive metal, conductive alloy, graphite, etc., including, but not limited to, consumable electrode materials (such as copper), inert materials (such as platinum, titanium, graphite), stainless steel, etc.
  • conductive metal such as conductive metal, conductive alloy, graphite, etc.
  • consumable electrode materials such as copper
  • inert materials such as platinum, titanium, graphite
  • stainless steel etc.
  • the electrode plug guide can be made of any suitable conductive material, such as conductive metal, conductive alloy, graphite, etc., including, but not limited to, consumable electrode materials (such as copper), inert materials (such as platinum, titanium, graphite), stainless steel, etc.
  • conductive metal such as conductive metal, conductive alloy, graphite, etc.
  • consumable electrode materials such as copper
  • inert materials such as platinum, titanium, graphite
  • stainless steel etc.
  • the present application provides a method for vertical electrophoresis of a sample, comprising:
  • the sample may contain nucleic acid molecules or protein molecules.
  • step ii) the size of the gel plate and the number of gel plates can be determined according to the amount of the sample to be tested, so as to select the corresponding detachable electrophoresis core.
  • the detachable electrophoresis core in step ii) can be a detachable electrophoresis core configured to be able to mount two gel plates, or a gel plate and a gel replacement plate, or a detachable electrophoresis core configured to be able to mount only one gel plate.
  • the present application also provides a method for performing vertical electrophoresis on a sample, comprising:
  • the sample may contain nucleic acid molecules or protein molecules.
  • step ii) the size of the gel plate and the number of gel plates can be determined according to the amount of the sample to be tested, so as to select the corresponding detachable electrophoresis core.
  • the number of the detachable electrophoresis cores in step ii) may be one or two.
  • the detachable electrophoresis core in step ii) may be a detachable electrophoresis core configured to be equipped with two gel plates, or a gel plate and a gel replacement plate, and/or a detachable electrophoresis core configured to be equipped with only one gel plate.
  • FIG. 1 is a top view of a detachable electrophoresis core of the present application.
  • FIG. 2 is a top view of a detachable electrophoresis core of the present application equipped with a gel plate.
  • FIG3 is a top view (A) and a partial front view (B) of another detachable electrophoresis core of the present application.
  • FIG. 4 is a top view of another detachable electrophoresis core of the present application equipped with a gel plate.
  • FIG. 5 is a top view of the electrophoresis tank containing two tank bodies of the present application.
  • FIG. 7 is a top view of an electrophoresis tank equipped with different numbers and types of detachable electrophoresis cores.
  • “Detachable” means that the connection between two or more objects is not permanent but can be To connect or disconnect.
  • the "detachable” electrophoresis core in this article refers to an electrophoresis core that can be assembled into the electrophoresis tank as needed and can be removed from the electrophoresis tank as needed.
  • the detachable electrophoresis core can be assembled into the electrophoresis tank before the start of electrophoresis and removed from the electrophoresis tank after the end of electrophoresis.
  • Conductive materials refer to materials that can be used to transport and conduct electric current, such as conductive metals, conductive alloys, and graphite.
  • Metals generally refer to a class of substances that have a unique luster, opacity, ductility, thermal conductivity, and electrical conductivity. Most of them have excellent electrical conductivity. Alloys refer to substances that are a mixture of two or more chemical substances (at least one of which is a metal) and have metallic properties.
  • “Inert” metals generally refer to metals with low activity and strong resistance to oxidation and corrosion, including gold, silver, and the six platinum group elements (ruthenium, rhodium, palladium, osmium, iridium, and platinum).
  • stainless steel refers to metal alloys that are passive, corrosion-resistant, and rust-free in the atmosphere and corrosive media such as acids, alkalis, and salts. They usually contain a high content (12% to 30%) of chromium and contain elements such as nickel, molybdenum, vanadium, manganese, and tungsten. Because they contain metals, they must be conductive. Graphite is an allotrope of carbon, which can be crystalline, etc. It is conductive due to the presence of a large number of delocalized electrons in the crystal structure.
  • “Substantially cuboid” means that the shape or outline of the object is close to a cuboid, but not a regular cuboid.
  • An object that is “substantially cuboid” may have local deformations on the basis of the cuboid, such as a cylindrical protrusion on one side of the cuboid, a concave inward on one side of the cuboid, etc.
  • a rectangular or substantially rectangular object such as the slot base of the present application, has a long side that refers to the longer side of the rectangle or substantially rectangle presented by the top or bottom surface of the object, and a short side that refers to the shorter side of the rectangle or substantially rectangle presented by the top or bottom surface of the object.
  • “Matching” or “matching” in this application means that the shapes, sizes, etc. of two objects can enable the two objects to be tightly combined together in a manner such as engagement, meshing, embedding, etc.
  • the groove on the tank base can match the protrusion on the inner core frame, thereby vertically fixing the removable electrophoresis inner core to the electrophoresis tank.
  • “matching” or “matching” can mean that the shapes, sizes, etc. of two objects are in a basically identical and interchangeable state.
  • the size of the gel substitute plate can match the gel plate, which means that the shape and size of the gel substitute plate are basically the same as the gel plate, and can replace the gel plate and the inner core frame to form a sealed tank body.
  • “Sealed” in this application means that two objects are tightly joined so that no gas or liquid can flow from the joint of the two objects.
  • the gel plate or gel substitute plate in this application can be assembled into the inner core frame to form a sealed electrophoresis inner tank with the inner core frame, and the electrophoresis buffer poured into it will not flow out of the inner tank.
  • “embedded” in an object means firmly fixed in the object, which can be a state of being completely embedded in the object or in contact with the outside world.
  • the electrode "embedded" in the tank base of this application can be completely embedded in the plate of the tank base and cannot be in contact with the outside air, or the plate of the tank base fixes the electrode in a semi-enclosed form so that the electrode can be in contact with the outside air, as long as the condition that at least a part of the electrode can be in contact with the electrophoresis buffer is met.
  • “Vertical” refers to the direction perpendicular to the horizontal plane, parallel to the direction of gravity. “Horizontal plane” refers to the plane formed by the surface of water when it is still under the action of gravity, which is perpendicular to the direction of gravity. “Horizontal direction” refers to the direction parallel to the horizontal plane, perpendicular to the vertical direction.
  • “Vertical electrophoresis” refers to electrophoresis in which the gel is placed vertically and nucleic acid or protein molecules are separated in the vertical direction.
  • Two or more objects that are “electrically connected” allow current to flow, or the fact that current flows, when an external voltage is applied.
  • Two or more objects that are “capable of being electrically connected” allow current to flow, or the fact that current flows, when an external voltage is applied, after being connected to each other.
  • top end portion refers to the end portion on the top surface of the side panel.
  • midi type electrophoresis devices There are roughly two types of vertical electrophoresis devices on the market, namely midi type and mini type. Considering the volume factor and the amount of electrophoresis buffer used, midi type electrophoresis devices all adopt an integral design Midi electrophoresis tank, whose "inner core" cannot be disassembled and can only be equipped with one gel plate.
  • the gel used is usually larger in size, such as about 10cm ⁇ 15cm.
  • the electrophoresis core of the mini type electrophoresis device is usually detachable, and the first electrode, the second electrode, the first electrode plug, and the second electrode plug are all set on the electrophoresis core. At most two gel plates can be assembled, and the gel used is usually smaller in size, such as about 10cm ⁇ 10cm.
  • a mini electrophoresis device or a midi electrophoresis device.
  • one electrode is installed inside the inner core frame, and the other electrode (usually the positive electrode) is installed on the bottom and outside of the inner core frame and is usually exposed.
  • the positive electrode is usually made of platinum, which is relatively expensive.
  • a new vertical electrophoresis device which may include:
  • an electrophoresis tank which may include a tank base and an upper cover, wherein the tank base may include a first electrode, a first electrode plug, a second electrode plug, and an electrode plug guide, and
  • each set of detachable electrophoresis cores may include more than one detachable electrophoresis core, wherein each detachable electrophoresis core may include a core frame, and a second electrode, wherein the core frame may be configured to assemble a gel plate or a gel replacement plate,
  • the first electrode can be configured to be connected to the first electrode plug and can be electrically connected to the first electrode plug,
  • the second electrode may be configured to be electrically connected to the electrode plug guide, and the electrode plug guide may be configured to be connected to the second electrode plug and be electrically connected to the second electrode plug.
  • the first electrode can be located at any position of the tank base, for example, it can be set on the side plate of the tank base, or the side plate plus the bottom plate.
  • the electrode usually located on the outside and bottom side of the electrophoresis core (such as the first electrode in this application) on the tank base, especially on the side plate and bottom plate of the tank base, by attachment or embedding the collision damage of the electrode can be significantly reduced, the service life of the electrophoresis device can be extended, and the user experience can be improved.
  • the second electrode plug is arranged on the tank base and connected to the electrophoresis inner
  • the second electrode on the core is electrically connected.
  • Each detachable electrophoresis core in each group of detachable electrophoresis cores in the electrophoresis device has the same structure, and the sizes of the gel plates or gel replacement plates that can be installed are also the same.
  • each group of electrophoresis cores can be configured to install gel plates or gel replacement plates of different sizes, for example, one group of cores is configured to install a gel plate or gel replacement plate of about 10cm ⁇ 10cm, and the other group of cores is configured to install a gel plate or gel replacement plate of about 10cm ⁇ 15cm, thereby expanding the adaptability range of the electrophoresis device to the gel plate.
  • the mini type electrophoresis core that is, the electrophoresis core that can be equipped with at most two gel plates, has its second electrode usually located on the center line of the core frame.
  • the midi type electrophoresis core that is, the electrophoresis core that can only be equipped with at most one gel plate, has its second electrode usually located on the side plate opposite to the gel plate rather than on the center line of the core frame.
  • the electrophoresis device when the electrophoresis device is to be compatible with the midi type electrophoresis core and the mini type electrophoresis core, according to the original design, that is, without changing the structure of the midi and mini cores, including but not limited to the arrangement position of the second electrode plug, the distribution method of the second electrode, and the connection method between the second electrode plug and the second electrode, the mini type core needs to be installed in the middle part of the tank base, and the midi type core needs to be installed in one half of the tank base. In this way, a larger tank base volume is required to meet this arrangement.
  • the volume of the tank base can be greatly reduced, the floor space can be reduced, and the amount of electrophoresis buffer used in the electrophoresis outer tank can be reduced.
  • the tank base of the present application can be configured to just accommodate midi-type and mini-type electrophoresis cores.
  • "just enough to accommodate” means that after the tank base accommodates any type of electrophoresis core, there is almost no extra internal space.
  • the first electrode plug and the second electrode plug can be respectively arranged at the two top ends of a side plate including the long side of the tank base, and the electrode plug guide can be arranged at the top of the same side plate or the side plate adjacent to the side plate close to the second electrode plug and protrude into the tank base.
  • the electrode plug guide and the second electrode can be connected at a higher position to prevent accidental contact with the electrophoresis buffer in the electrophoresis outer tank.
  • the electrode plug guide can protrude into the tank base in the horizontal direction.
  • the electrode plug guide can protrude into the tank base in the horizontal direction in a direction parallel to the side plate on which the first electrode plug and the second electrode plug are arranged.
  • one end of the second electrode can be configured to extend outward from a side plate adjacent to the gel plate in a horizontal direction on the top surface of the frame body of the core frame in a direction perpendicular to the side plate and extend after bending, presenting a " ⁇ " shape, or to extend outward and extend in both directions after bending, presenting a "T" shape.
  • the two ends of the second electrode can be respectively extended outward from two side plates adjacent to the gel plate in a direction perpendicular to the two side plates on the top surface of the frame body in a centrally symmetrical manner relative to the center of the top surface of the frame body in a horizontal direction on the top surface of the frame body and extend after bending, presenting a " ⁇ " shape, or to extend outward and extend in both directions after bending. After that, it extends in both directions to present a "T" shape.
  • the endpoint of the second electrode extends in a " ⁇ " shape
  • one end of the second electrode extends outward in a "T” shape
  • the two ends extend outward in a "T" shape in a centrally symmetrical manner, there is no need to make much judgment during the preparation and installation process, and "fool-style" assembly can be easily achieved.
  • one end of the second electrode can be extended outward from the side plate opposite to the gel plate in the horizontal direction on the top surface of the frame body.
  • one end of the second electrode can be extended outward from the side plate opposite to the gel plate in the horizontal direction on the top surface of the frame body in a direction parallel to the side plate.
  • the two ends of the second electrode can be extended outward from the side plate opposite to the gel plate in the horizontal direction on the top surface of the frame body in a mirror-symmetrical manner relative to the plane passing through the midpoint of the top edge of the gel plate and perpendicular to the gel plate.
  • the detachable electrophoresis core is configured to be able to assemble only one gel plate, and the two ends of the second electrode can be extended outward from the side plate opposite to the gel plate in the top surface of the frame body in a mirror-symmetrical manner relative to a plane passing through the midpoint of the top edge of the gel plate and perpendicular to the gel plate in the horizontal direction in a direction parallel to the side plate.
  • the bottom plate of the frame body of the electrophoresis core and/or the two side plates can have a larger thickness.
  • the larger thickness can be 1.0 cm, 1.5 cm, 2.0 cm, or 2.5 cm.
  • the thickness of the bottom plate and the two side plates of the frame body can be larger, for example, each can be 1.0 cm, 1.5 cm, 2.0 cm, or 2.5 cm.
  • the two types of electrophoresis inner cores can be compatible and the volume of the tank base can be minimized.
  • the first electrode plug and the second electrode plug can be arranged on the top of the two side panels including the short sides of the tank base in a mirror-symmetrical manner relative to the connecting line of the centers of the two long sides of the tank base, and the electrode plug guide can be arranged on the top of the side panel on which the second electrode plug is arranged and protrudes into the tank base.
  • the first electrode plug and the second electrode plug can be respectively arranged at the basic center of the top of the two side panels including the short sides of the tank base, and the electrode plug guide can be arranged on the top of the side panel on which the second electrode plug is arranged and protrudes into the tank base.
  • Each detachable electrophoresis core including a detachable electrophoresis core configured to be able to assemble two gel plates, or a gel plate and a gel replacement plate, and a detachable electrophoresis core configured to be able to assemble only one gel plate, can extend one end of the second electrode outward from a side plate adjacent to the gel plate in a horizontal direction on the top surface of the frame body in a direction parallel to the gel plate, or can extend the two ends of the second electrode in a centrally symmetrical manner relative to the center of the top surface of the frame body. In a manner, the top surface of the frame body extends outward in a horizontal direction from two side plates adjacent to the gel plate in a direction parallel to the gel plate.
  • the inner core frame may also include a first protrusion and a second protrusion extending outwardly from two side plates of the frame body in a horizontal direction in a direction parallel to the gel plates on the top surface of the frame body.
  • the first protrusion and the second protrusion may match the structure on the tank base, such as a groove, so as to vertically fix the electrophoresis device in the electrophoresis tank.
  • An end portion of the second electrode extending outward from the side plate may be arranged on the first protrusion and configured to protrude outward from the first protrusion.
  • the two ends of the second electrode may be arranged on the first protrusion and the second protrusion, respectively, and configured to protrude outward from the first protrusion and the second protrusion.
  • the core frame also includes a third protrusion and a fourth protrusion extending outwardly from two side plates of the frame body adjacent to the gel plate in a horizontal direction in a direction parallel to the gel plate on the top surface of the frame body.
  • the third protrusion and the fourth protrusion can match the structure on the tank base, such as a groove, so as to vertically fix the electrophoresis device in the electrophoresis tank.
  • the length of the edge of the third protrusion and the fourth protrusion connected to the two side plates can be less than the length of the edge of the two side plates connected to the third protrusion and the fourth protrusion. In this way, the third protrusion and the fourth protrusion will not affect, for example, block the connection between the end of the second electrode and the electrode plug guide, and make the tank base "just" able to accommodate any type of electrophoresis core.
  • the second electrode except for one or two ends having the above-mentioned special configuration, the other parts can be located at any position of the inner core frame, and is configured so that at least a part of it contacts the electrophoresis buffer when the electrophoresis buffer is poured into the sealed groove formed by the inner core frame and the gel plate.
  • the part of the second electrode that contacts the electrophoresis buffer can be attached to or embedded in the side plate of the frame body opposite to the gel plate at a height slightly lower than the short plate of the gel plate.
  • the part of the second electrode that contacts the electrophoresis buffer can be attached to or embedded in the two side plates of the frame body adjacent to the gel plate at the height of the short plate of the gel plate, and span the frame body. Based on this design, the distance between the second electrode and the loading hole of the gel is shorter, which can improve the electrophoresis efficiency.
  • the width of the portion of the second electrode that contacts the electrophoresis buffer is smaller than the width of one or two side plates of the frame body that are adjacent to the gel plate. In this way, the electrode can be prevented from contacting the gel plate or the gel replacement plate, and collision damage to the electrode can be reduced.
  • the first electrode in the present application can be a positive electrode, which can be made of a stainless steel material with an inert conductive metal (such as gold, platinum, iridium, tantalum) coated on the surface.
  • the second electrode can be a negative electrode, which can be made of stainless steel. Replacing the platinum wire with a stainless steel material can enhance the strength of the electrode, extend the service life, and reduce the cost.
  • the stainless steel electrode can enhance the structural strength and stability of the electrophoresis core and the tank base.
  • the present application also provides a vertical electrophoresis device comprising a double tank.
  • the structure of the vertical electrophoresis device is basically the same as
  • the single tank electrophoresis apparatus is identical except that the tank base includes two tank bodies and the electrode plug and the electrode plug guide are arranged differently.
  • the tank base may include a first tank body and a second tank body separated, the first tank body and the second tank body may both be substantially rectangular, the first tank body and the second tank body may be arranged in a manner that the long sides are opposite and the short sides are in a line, and share a side plate.
  • the first electrode plug and the second electrode plug may be respectively arranged at the two top ends of the side plate shared by the first tank body and the second tank body, and the electrode plug guide may be arranged at the top of the common side plate or the side plate adjacent to the side plate close to the second electrode plug and protrude into the first tank body and the second tank body.
  • the first electrode may be configured so that at least a portion of it contacts the electrophoresis buffer to be added in the first tank body, and at least a portion of it contacts the electrophoresis buffer to be added in the second tank body.
  • the vertical electrophoresis device may include a) one or more groups of detachable electrophoresis cores, each detachable electrophoresis core in each group of detachable electrophoresis cores can be configured to assemble two gel plates, or one gel plate and a gel replacement plate; b) one or more groups of detachable electrophoresis cores, each detachable electrophoresis core in each group is configured to be able to assemble only one gel plate; or c) two or more groups of detachable electrophoresis cores, each detachable electrophoresis core in one or more groups of detachable electrophoresis cores is configured to be able to assemble two gel plates, or one gel plate and a gel replacement plate, and each detachable electrophoresis core in one or more groups of detachable electrophoresis cores is configured to be able to assemble only one gel plate.
  • one end of the second electrode can be configured to extend outward from a side plate adjacent to the gel plate in a horizontal direction on the top surface of the frame body of the core frame in a direction perpendicular to the side plate and extend after bending, presenting a " ⁇ " shape, or extend outward and extend in two directions after bending, presenting a "T" shape.
  • the two ends of the second electrode can be respectively extended outward from two side plates adjacent to the gel plate in a direction perpendicular to the two side plates on the top surface of the frame body in a centrally symmetrical manner relative to the center of the top surface of the frame body in a horizontal direction on the top surface of the frame body and extend after bending, presenting a " ⁇ " shape, or extend outward and extend in two directions after bending, presenting a "T" shape.
  • the two ends of the second electrode can be extended outward from the side plate opposite to the gel plate in a horizontal direction on the top surface of the frame body in a mirror-symmetrical manner relative to a plane passing through the midpoint of the top edge of the gel plate and perpendicular to the gel plate.
  • the two ends of the second electrode can be extended outward from the side plate opposite to the gel plate in a horizontal direction on the top surface of the frame body in a mirror-symmetrical manner relative to a plane passing through the midpoint of the top edge of the gel plate and perpendicular to the gel plate. Only mirror Only a symmetrical design can ensure that the electrophoresis core can be connected to the electrode plug guide when it is in the first trough body and the second trough body.
  • FIG1 shows an embodiment of a detachable electrophoresis core configured to be able to assemble two gel plates, or one gel plate and one gel replacement plate
  • FIG2 shows a schematic diagram of the detachable electrophoresis core after being assembled into a gel plate.
  • the electrophoresis inner core frame 2 includes a second electrode 1, and the two ends 3 and 4 of the second electrode 1 are arranged in a bilaterally symmetrical manner.
  • the second electrode 1 i.e., the negative electrode
  • the second electrode 1 is integrated into the inner core frame 2 by means of encapsulation injection molding or interlocking installation, and is connected to the electrophoresis buffer in the electrophoresis inner tank 11 by exposing the inner side of the electrophoresis inner core frame 2 by opening the glue or half-encapsulation.
  • the second electrode 1 can be made of stainless steel material, including but not limited to 204, 304, 314, or 316, etc., and processed into a " ⁇ " shape.
  • the inner wall of the inner core frame is provided with a U-shaped beam frame, and a single-stage or multi-stage U-shaped groove or a semicircular groove 5 is opened on one side to facilitate the installation of a sealing strip 6, such as a flexible silicone or foam sealing strip.
  • FIG. 3 shows an embodiment of the detachable electrophoresis core of the present application that is configured to be able to assemble only one gel plate
  • FIG. 4 shows a schematic diagram of the detachable electrophoresis core after being assembled into a gel plate.
  • the second electrode i.e., the negative electrode
  • the second electrode is integrated into the inner core frame 13 by means of overmolding or interlocking assembly, with its two ends 15 and 16 exposed, and the second electrode is connected to the electrophoresis buffer in the electrophoresis inner tank 21.
  • Single-stage or multi-stage U-shaped grooves or semicircular grooves are opened on both sides of the inner core frame 13 to facilitate the installation of a sealing strip 17, such as a flexible silicone or foam sealing strip.
  • the inner core frame 13 also includes a gel plate or gel replacement plate fastener 14, such as a C-shaped buckle connected to the inner core frame 13 by a bottom hinge, and the C-shaped buckle can rotate around the hinge axis within a range of 0 to 210 degrees.
  • a gel plate or gel replacement plate fastener 14 such as a C-shaped buckle connected to the inner core frame 13 by a bottom hinge, and the C-shaped buckle can rotate around the hinge axis within a range of 0 to 210 degrees.
  • FIG5 shows an embodiment of the electrophoresis tank of the present application.
  • FIG6 shows the electrophoresis tank after being assembled into a detachable Schematic diagram after electrophoresis core.
  • the tank base 25 of the electrophoresis tank is designed as a chamber, and adopts an integrally designed crosspiece, that is, a common side plate 29 of the two chambers, to divide the tank base into two independent inner chambers, namely, a first tank body 27 and a second tank body 28.
  • a negative electrode spring, namely, an electrode plug guide 23, and a second electrode plug 26 are installed on one side of the tank base; and a first electrode 24 (i.e., a positive electrode) and a first electrode plug 30 are installed on the other side.
  • the electrode plug guide 23 adopts a high-rise design to prevent it from accidentally contacting the electrophoresis buffer of the outer tank.
  • the positive electrode material is made of metal (including but not limited to stainless steel, or copper, iron, titanium and other metals or their alloys) covered with inert conductive metals (including but not limited to gold, platinum, iridium, tantalum, etc.) through surface treatment (including but not limited to electroplating, spraying and other processes), rather than metal platinum wire, which can effectively reduce the loss of the positive electrode and provide good mechanical properties, greatly reduce the difficulty of production and assembly, and reduce the accidental breakage of the platinum wire during use.
  • the positive electrode adopts a Z-shaped structure, and the conductive part is deeply inserted into the bottom of the tank base.
  • the first electrode 24 is connected to the first electrode plug 30
  • the electrode plug guide 23 is connected to the second electrode plug 26 .
  • the detachable electrophoresis core is assembled into the tank base 25, and the second electrode on the detachable electrophoresis core is connected to the electrode plug guide 23 via one of the two ends 3 and 4, or one of 15 and 16.
  • the detachable electrophoresis core is assembled into the first tank body 27 and/or the second tank body 28, so that the second electrode on the detachable electrophoresis core is connected to the electrode plug guide 23 via one of the two ends 3 and 4, or one of 15 and 16.
  • the upper cover of the electrophoresis tank is covered, so that the first electrode plug 30 and the second electrode plug 26 are respectively connected to the first power interface and the second power interface on the upper cover, and the power interface can be connected to the electrophoresis instrument via a cable.
  • the two symmetrical ends 3 and 4 of the second electrode can ensure that when the electrophoresis core is placed in the electrophoresis tank in any direction, the electrophoresis buffer in the electrophoresis tank is connected to the negative electrode.
  • This design allows users to avoid the installation direction of the electrode core without considering the installation direction of the electrode core, and can fundamentally avoid the error of reverse electrode connection without additional fool-proofing design.
  • the two symmetrical ends 15 and 16 of the second electrode can ensure that when the electrophoresis core is placed in the electrophoresis tank in any direction, the electrophoresis buffer in the electrophoresis tank is connected to the negative electrode.
  • the electrophoresis tank of the present application is compatible with both Mini and Midi electrophoresis cores.
  • FIG7 in a dual-tank electrophoresis tank, it can support 1-2 midi gels to be electrophoresed separately or simultaneously, and the two gels do not affect each other; support 1-4 mini gels to be electrophoresed separately or simultaneously, and the electrophoresis between independent tanks does not affect each other; or support 1 midi gel and 1-2 mini gels to be electrophoresed simultaneously, and the electrophoresis between independent tanks does not affect each other.
  • an electrophoresis outer tank 12 is formed between the tank base and the electrophoresis inner core.
  • Electrophoresis buffer is injected into the electrophoresis inner tank and the electrophoresis outer tank respectively.
  • the electrophoresis inner tank needs to be filled with electrophoresis buffer to ensure that the buffer is in full contact with the upper end of the gel.
  • the electrophoresis outer tank can be injected to about 2/3 of the height of the gel plate to ensure a good heat dissipation effect, but it cannot be at the same height as the electrophoresis liquid level in the electrophoresis inner tank to avoid short circuit between the inner and outer tanks.
  • the second electrode is connected to the electrode plug guide 23 via the end 3/4 or 15/16 to connect the negative pole of the power supply to the electrophoresis buffer in the electrophoresis inner tank.
  • the electrophoresis buffer in the electrophoresis outer tank is connected to the positive pole power supply through the first electrode 24 in the tank base.
  • the upper and lower ends of the gel are connected to the negative pole and the positive pole of the power supply through the electrophoresis buffer of the inner and outer tanks of the electrophoresis, respectively, to form a voltage difference, driving the nucleic acid or protein molecules to migrate in the gel.
  • the vertical electrophoresis device of the present application has the following advantages: i) the tank base is designed with a separate partition to form two independent outer tanks that do not affect each other, which can be used independently or simultaneously, ensuring heat dissipation and effectively reducing the amount of electrophoresis buffer used; ii) the negative electrode and the positive electrode are respectively integrated on the electrophoresis core and the tank base, so that the two electrophoresis units are completely independent and do not affect each other; iii) the electrodes on the mini and midi electrophoresis cores are designed symmetrically to achieve a unified size and electrode interface; with the uniquely designed power-on device, the electrophoresis core can be placed at will, which greatly facilitates the user's operation and fundamentally eliminates the risk of electrode inversion; iv) it is compatible with both mini and midi sizes of gel, and supports mixed mode to achieve The mixed use mode of midi and mini can support electrophoresis of up to 4 mini gels at the same time,

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  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
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Abstract

L'invention concerne un appareil d'électrophorèse verticale, comprenant i) un réservoir d'électrophorèse, et ii) au moins un groupe de noyaux internes d'électrophorèse détachables, une base de cuve (25) de la cuve d'électrophorèse comprenant une première électrode (24), une première fiche d'électrode (30), une seconde fiche d'électrode (26) et une pièce de guidage de fiche d'électrode (23) ; chaque groupe de noyaux internes d'électrophorèse amovibles comprend au moins une plaque de gel d'électrophorèse détachable, et chaque noyau interne d'électrophorèse détachable comprend des cadres de noyau interne (2, 13) et une seconde électrode (1), les cadres de noyau interne (2, 13) étant conçus pour pouvoir assembler la plaque de gel ou une plaque de remplacement de gel ; la première électrode (24) est configurée pour se connecter à la première fiche d'électrode (30) et peut être électriquement connectée à celle-ci ; la seconde électrode (1) est configurée pour se connecter électriquement à la pièce de guidage de fiche d'électrode (23) ; et la pièce de guidage de fiche d'électrode (23) est configurée pour se connecter à la seconde fiche d'électrode (26) et peut être électriquement connectée à celle-ci (26).
PCT/CN2024/126587 2023-10-23 2024-10-23 Nouvel appareil d'électrophorèse verticale et son utilisation Pending WO2025087247A1 (fr)

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CN202311376763 2023-10-23
CN202311376763.1 2023-10-23

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050103628A1 (en) * 2003-09-22 2005-05-19 Jackson Thomas R. Apparatus for concurrent electrophoresis in a plurality of gels
US20060254918A1 (en) * 2005-04-15 2006-11-16 Jackson Tom R Expanding cam lock for sealing slab gels in an electrophoresis apparatus
US20100326830A1 (en) * 2009-06-26 2010-12-30 Yi Wang Monolithic electrophoresis gel system
CN206479488U (zh) * 2017-01-11 2017-09-08 北京凯元信瑞仪器有限公司 具有防触电功能的直接接触式循环控温式电泳槽
CN108490059A (zh) * 2018-05-03 2018-09-04 莫纳（苏州）生物科技有限公司 一种垂直电泳槽及电泳仪

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050103628A1 (en) * 2003-09-22 2005-05-19 Jackson Thomas R. Apparatus for concurrent electrophoresis in a plurality of gels
US20060254918A1 (en) * 2005-04-15 2006-11-16 Jackson Tom R Expanding cam lock for sealing slab gels in an electrophoresis apparatus
US20100326830A1 (en) * 2009-06-26 2010-12-30 Yi Wang Monolithic electrophoresis gel system
CN206479488U (zh) * 2017-01-11 2017-09-08 北京凯元信瑞仪器有限公司 具有防触电功能的直接接触式循环控温式电泳槽
CN108490059A (zh) * 2018-05-03 2018-09-04 莫纳（苏州）生物科技有限公司 一种垂直电泳槽及电泳仪

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