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WO2025083418A3 - Methods for cell culturing - Google Patents

Methods for cell culturing Download PDF

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Publication number
WO2025083418A3
WO2025083418A3 PCT/GB2024/052677 GB2024052677W WO2025083418A3 WO 2025083418 A3 WO2025083418 A3 WO 2025083418A3 GB 2024052677 W GB2024052677 W GB 2024052677W WO 2025083418 A3 WO2025083418 A3 WO 2025083418A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
cellular
vitro
cell culturing
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/GB2024/052677
Other languages
French (fr)
Other versions
WO2025083418A2 (en
Inventor
Ricardo GOUVEIA
Che Connon
Craig STAMP
Emily TELFORD
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
3D Bio Tissues Ltd
Original Assignee
3D Bio Tissues Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 3D Bio Tissues Ltd filed Critical 3D Bio Tissues Ltd
Publication of WO2025083418A2 publication Critical patent/WO2025083418A2/en
Publication of WO2025083418A3 publication Critical patent/WO2025083418A3/en
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1305—Adipocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1335—Skeletal muscle cells, myocytes, myoblasts, myotubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C12N5/0659—Satellite cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Rheumatology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a novel composition for cultivating animal biomass in vitro. Corresponding methods are also provided herein. Further, this invention relates to a cellular composite for cultivating animal biomass in vitro, wherein the cellular composite comprises a cellular substrate and the composition of the invention. Corresponding methods for generating the cellular composite are also provided herein. Finally, a method for cultivating animal biomass in vitro in serum-free or reduced-serum conditions using the composition and cellular substrate of the invention are also provided.
PCT/GB2024/052677 2023-10-19 2024-10-18 Methods for cell culturing Pending WO2025083418A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB2316002.1 2023-10-19
GBGB2316002.1A GB202316002D0 (en) 2023-10-19 2023-10-19 Methods for cell culturing

Publications (2)

Publication Number Publication Date
WO2025083418A2 WO2025083418A2 (en) 2025-04-24
WO2025083418A3 true WO2025083418A3 (en) 2025-05-22

Family

ID=88970268

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2024/052677 Pending WO2025083418A2 (en) 2023-10-19 2024-10-18 Methods for cell culturing

Country Status (2)

Country Link
GB (1) GB202316002D0 (en)
WO (1) WO2025083418A2 (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019016795A1 (en) * 2017-07-15 2019-01-24 Technion Research & Development Foundation Limited Cultured meat compositions
US20200140821A1 (en) * 2017-06-07 2020-05-07 Wild Type, Inc. Ex vivo meat production
WO2020123876A1 (en) * 2018-12-12 2020-06-18 Wild Type, Inc. Synthetic food compositions
CN114438014A (en) * 2022-01-26 2022-05-06 上海食未生物科技有限公司 An edible chitosan glutenin biomimetic oriented cell culture meat bioscaffold
US20220195392A1 (en) * 2019-04-23 2022-06-23 The Regents Of The University Of California Methods and compositions for cell culture on heterogeneous scaffolds
WO2023285813A1 (en) * 2021-07-12 2023-01-19 3D Bio-Tissues Limited Cell culture medium and supplements for cellular meat production
US20230115567A1 (en) * 2017-04-09 2023-04-13 Supermeat The Essence Of Meat Ltd. Cultured meat-containing hybrid food

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102690364B (en) 2012-06-03 2014-07-02 柳州爱格富食品科技股份有限公司 Ficoll synthesis process method

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230115567A1 (en) * 2017-04-09 2023-04-13 Supermeat The Essence Of Meat Ltd. Cultured meat-containing hybrid food
US20200140821A1 (en) * 2017-06-07 2020-05-07 Wild Type, Inc. Ex vivo meat production
WO2019016795A1 (en) * 2017-07-15 2019-01-24 Technion Research & Development Foundation Limited Cultured meat compositions
WO2020123876A1 (en) * 2018-12-12 2020-06-18 Wild Type, Inc. Synthetic food compositions
US20220195392A1 (en) * 2019-04-23 2022-06-23 The Regents Of The University Of California Methods and compositions for cell culture on heterogeneous scaffolds
WO2023285813A1 (en) * 2021-07-12 2023-01-19 3D Bio-Tissues Limited Cell culture medium and supplements for cellular meat production
CN114438014A (en) * 2022-01-26 2022-05-06 上海食未生物科技有限公司 An edible chitosan glutenin biomimetic oriented cell culture meat bioscaffold

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DAVID SHLOMIT ET AL: "Co-culture approaches for cultivated meat production", NATURE REVIEWS BIOENGINEERING, 12 June 2023 (2023-06-12), XP093055295, Retrieved from the Internet <URL:https://www.nature.com/articles/s44222-023-00077-x> DOI: 10.1038/s44222-023-00077-x *
JEONG H J ET AL: "Coculture with BJ fibroblast cells inhibits the adipogenesis and lipogenesis in 3T3-L1 cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM NL, vol. 392, no. 4, 19 February 2010 (2010-02-19), pages 520 - 525, XP026912698, ISSN: 0006-291X, [retrieved on 20100218], DOI: 10.1016/J.BBRC.2009.12.184 *

Also Published As

Publication number Publication date
WO2025083418A2 (en) 2025-04-24
GB202316002D0 (en) 2023-12-06

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