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WO2025072834A3 - Procédé de séquençage et de synthèse de polynucléotide en forme de l - Google Patents

Procédé de séquençage et de synthèse de polynucléotide en forme de l Download PDF

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Publication number
WO2025072834A3
WO2025072834A3 PCT/US2024/049074 US2024049074W WO2025072834A3 WO 2025072834 A3 WO2025072834 A3 WO 2025072834A3 US 2024049074 W US2024049074 W US 2024049074W WO 2025072834 A3 WO2025072834 A3 WO 2025072834A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequencing
synthesizing
polynucleotide
nucleotides
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/049074
Other languages
English (en)
Other versions
WO2025072834A2 (fr
Inventor
Dae Hyun Kim
Bo Shun HUANG
Jun Hyuk CHUNG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dxome Co Ltd
Original Assignee
Dxome Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dxome Co Ltd filed Critical Dxome Co Ltd
Publication of WO2025072834A2 publication Critical patent/WO2025072834A2/fr
Publication of WO2025072834A3 publication Critical patent/WO2025072834A3/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/14—Pyrrolo-pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente divulgation concerne un nouveau procédé d'amplification, de séquençage ou de synthèse de polynucléotide en forme de L. L'invention concerne en outre des nucléotides en forme de L et des enzymes en forme de D qui peuvent être utilisées dans le procédé. L'invention concerne également le procédé de fabrication des nucléotides en forme de L et des enzymes en forme de D.
PCT/US2024/049074 2023-09-27 2024-09-27 Procédé de séquençage et de synthèse de polynucléotide en forme de l Pending WO2025072834A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363585885P 2023-09-27 2023-09-27
US63/585,885 2023-09-27

Publications (2)

Publication Number Publication Date
WO2025072834A2 WO2025072834A2 (fr) 2025-04-03
WO2025072834A3 true WO2025072834A3 (fr) 2025-06-12

Family

ID=93150549

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/049074 Pending WO2025072834A2 (fr) 2023-09-27 2024-09-27 Procédé de séquençage et de synthèse de polynucléotide en forme de l

Country Status (1)

Country Link
WO (1) WO2025072834A2 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170137869A1 (en) * 2015-11-06 2017-05-18 Intelligent Biosystems, Inc. Methods for synthesizing nucleotide analogues with disulfide linkers
WO2017177759A1 (fr) * 2015-08-18 2017-10-19 清华大学 Système de réplication d'acide nucléique miroir
WO2022029512A1 (fr) * 2020-08-06 2022-02-10 Tsinghua University Synthèse chimique de grandes protéines d'image miroir et utilisations associées
WO2023183345A2 (fr) * 2022-03-22 2023-09-28 Dxome Inc. Procédé de séquençage de l-polynucléotide

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017177759A1 (fr) * 2015-08-18 2017-10-19 清华大学 Système de réplication d'acide nucléique miroir
US20170137869A1 (en) * 2015-11-06 2017-05-18 Intelligent Biosystems, Inc. Methods for synthesizing nucleotide analogues with disulfide linkers
WO2022029512A1 (fr) * 2020-08-06 2022-02-10 Tsinghua University Synthèse chimique de grandes protéines d'image miroir et utilisations associées
WO2023183345A2 (fr) * 2022-03-22 2023-09-28 Dxome Inc. Procédé de séquençage de l-polynucléotide

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KUZNETSOVA VIKTORIYA E ET AL: "Novel fluorescently labeled nucleotides: synthesis, spectral properties and application in polymerase chain reaction", MENDELEEV COMMUNICATIONS, INSTITUTE OF PHYSICS PUBLISHING, BRISTOL, GB, vol. 26, no. 2, 3 April 2016 (2016-04-03), pages 95 - 98, XP029492540, ISSN: 0959-9436, DOI: 10.1016/J.MENCOM.2016.03.002 *
SHERSHOV VALERIY E ET AL: "Synthesis and characterization of novel zwitterionic heptamethine indocyanine fluorophores", MENDELEEV COMMUNICATIONS, INSTITUTE OF PHYSICS PUBLISHING, BRISTOL, GB, vol. 27, no. 4, 29 July 2017 (2017-07-29), pages 360 - 362, XP085146053, ISSN: 0959-9436, DOI: 10.1016/J.MENCOM.2017.07.013 *

Also Published As

Publication number Publication date
WO2025072834A2 (fr) 2025-04-03

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