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WO2025072809A3 - Herbicide resistant hppd variants identified via directed evolution - Google Patents

Herbicide resistant hppd variants identified via directed evolution Download PDF

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Publication number
WO2025072809A3
WO2025072809A3 PCT/US2024/049038 US2024049038W WO2025072809A3 WO 2025072809 A3 WO2025072809 A3 WO 2025072809A3 US 2024049038 W US2024049038 W US 2024049038W WO 2025072809 A3 WO2025072809 A3 WO 2025072809A3
Authority
WO
WIPO (PCT)
Prior art keywords
mutated
gene
directed evolution
identified via
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/049038
Other languages
French (fr)
Other versions
WO2025072809A2 (en
Inventor
Huazhong Shi
Hongjia QIAN
Yihui YUAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Texas Tech University TTU
Texas Tech University System
Original Assignee
Texas Tech University TTU
Texas Tech University System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Texas Tech University TTU, Texas Tech University System filed Critical Texas Tech University TTU
Publication of WO2025072809A2 publication Critical patent/WO2025072809A2/en
Publication of WO2025072809A3 publication Critical patent/WO2025072809A3/en
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Embodiments of the present disclosure pertains to a modified plant or seed that includes a mutated 4-hydroxyphenylpyruvate dioxygenase (HPPD) gene. The mutated HPPD gene expresses an HPPD protein that confers herbicide resistance to the modified plant or seed. Additional embodiments of the present disclosure pertain to methods of producing modified plants or seeds by introducing a mutated HPPD gene to the plant or seed. The mutated HPPD gene may express a protein that includes SEQ ID NO: 1 or a sequence with at least 65% sequence identity to SEQ ID NO: 1. The mutated HPPD gene may also express a protein that includes SEQ ID NO: 2 or a sequence with at least 65% sequence identity to SEQ ID NO: 2.
PCT/US2024/049038 2023-09-30 2024-09-27 Herbicide resistant hppd variants identified via directed evolution Pending WO2025072809A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363541802P 2023-09-30 2023-09-30
US63/541,802 2023-09-30

Publications (2)

Publication Number Publication Date
WO2025072809A2 WO2025072809A2 (en) 2025-04-03
WO2025072809A3 true WO2025072809A3 (en) 2025-05-15

Family

ID=95201333

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/049038 Pending WO2025072809A2 (en) 2023-09-30 2024-09-27 Herbicide resistant hppd variants identified via directed evolution

Country Status (1)

Country Link
WO (1) WO2025072809A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6245968B1 (en) * 1997-11-07 2001-06-12 Aventis Cropscience S.A. Mutated hydroxyphenylpyruvate dioxygenase, DNA sequence and isolation of plants which contain such a gene and which are tolerant to herbicides
US20050289664A1 (en) * 2004-01-26 2005-12-29 Monsanto Technology, L.L.C. Genes encoding 4-Hydroxyphenylpyruvate dioxygenase (HPPD) enzymes for plant metabolic engineering
US20200063155A1 (en) * 2017-03-07 2020-02-27 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6245968B1 (en) * 1997-11-07 2001-06-12 Aventis Cropscience S.A. Mutated hydroxyphenylpyruvate dioxygenase, DNA sequence and isolation of plants which contain such a gene and which are tolerant to herbicides
US20050289664A1 (en) * 2004-01-26 2005-12-29 Monsanto Technology, L.L.C. Genes encoding 4-Hydroxyphenylpyruvate dioxygenase (HPPD) enzymes for plant metabolic engineering
US20200063155A1 (en) * 2017-03-07 2020-02-27 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use

Also Published As

Publication number Publication date
WO2025072809A2 (en) 2025-04-03

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