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WO2025071696A1 - Support de plaque à puits - Google Patents

Support de plaque à puits Download PDF

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Publication number
WO2025071696A1
WO2025071696A1 PCT/US2024/032534 US2024032534W WO2025071696A1 WO 2025071696 A1 WO2025071696 A1 WO 2025071696A1 US 2024032534 W US2024032534 W US 2024032534W WO 2025071696 A1 WO2025071696 A1 WO 2025071696A1
Authority
WO
WIPO (PCT)
Prior art keywords
support
length
well plate
width
level
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/032534
Other languages
English (en)
Inventor
Stephen C. Howard
Victoria A. TORRES
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
EMD Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EMD Millipore Corp filed Critical EMD Millipore Corp
Publication of WO2025071696A1 publication Critical patent/WO2025071696A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Definitions

  • Embodiments described herein relate to a support to prevent deformation of a well plate during centrifugation. More specifically, some embodiments relate to a support to prevent deformation of a well plate used in high resolution microscopy techniques, such as digital molecule counting, requiring a centrifugation step.
  • a lid sometimes referred as a 'shoe', to protect the bottom of a well plate from dust and debris when on a lab bench, such as the Aurora plates (product number: ABB200160A; sold by M il liporeS igma, Burlington, MA) packaged with a lid that can be used to protect the bottom of the plate or the Ultra Low Profile Lid for Microplates sold by Greiner Bio-One (product number: 691101 ) which can be used in a similar way with certain Greiner-brand well plates.
  • Aurora plates product number: ABB200160A; sold by M il liporeS igma, Burlington, MA
  • Greiner Bio-One product number: 691101
  • a support designed to physically support the optical read surface of a well plate to significantly reduce deformations and stresses, and thus diminish centrifugation issues would represent an advance in the art.
  • Some embodiments include a support for a well plate, the support comprising: a tiered rectangular structure comprising at least a top level and a lower level; a length and a width of the top level being less than a length and a width of the lower level, and the length and the width of the top level being at least the same as a length and a width of an optical read surface of the well plate; and the length and width of the lower level being greater than the length and the width of the top level, and the length and the width of the lower level are at least the same as a length and a width of the well plate.
  • the length of the lower level is within the range of 120 mm to 140 mm. In some embodiments, the width of the lower level is within the range of 75 mm to 95 mm. In some embodiments, the length of the top level is within the range of 105 mm to 130 mm. In some embodiments, the width of the top level is within the range of 65 mm to 85 mm. In some embodiments, at least one dimension of the support is such that at least one open space is formed between a top or a side of the top level or the lower level when the support is contacted with a well plate for centrifugation. In some embodiments, the support further comprises a middle level.
  • a length and a width of the top level being less than a length and a width of the middle level.
  • at least one dimension of the support is such that at least one open space is formed between a top or a side of the middle level.
  • the length of the middle level is within the range of 120 mm to 130 mm.
  • the width of the middle level is within the range of 80 mm to 90 mm.
  • Some embodiments include a method for reducing the frequency and degree of deformation or failure of a well plate during centrifugation compared to the risk of deformation or failure of the well plate without using the support of any one of claims 1-8 during centrifugation, the method comprising: contacting a well plate to an optical viewing area of the support; and centrifuging the well plate and support.
  • centrifuging occurs prior to single molecule counting.
  • the method further comprises preventing delamination of an optical read surface from a frame of the well plate.
  • FIG. 1 provides some embodiments of a well plate contacting a support.
  • FIG. 2 provides an exploded view of some embodiments shown in FIG. 1.
  • FIG. 3A provides a cross-section view of some embodiments shown in FIG. 1.
  • FIG. 3B provides a line drawing enlarging the portion of FIG. 3A within the circle.
  • FIG. 4 is a line drawing of a side view of some embodiments of the support.
  • the present disclosure describes some embodiments of a support to significantly reduce the frequency and degree of failure of well plates during centrifugation. Some embodiments of the support are especially helpful for reducing the frequency or degree of failure for glass bottom or transparent plastic bottom well plates, which are used in high resolution microscopy applications. Glass bottom or transparent plastic bottom well plates are generally more delicate than other types of well plates.
  • the optical read surface is adhesively attached to the well plate frame. The optical read surface can become delaminated if the frame deformation becomes significant, as is prone to happen during centrifugation.
  • the objective lens is positioned below the optical read surface when the contents of the wells in the well plate are being viewed using an inverted microscope.
  • a typical well plate has a thin frame that can subject to extreme bending and eventual failure at high centrifugal forces.
  • the support is below an optical read surface resulting in the well plate being more uniformly supported.
  • the optical read surface is flat.
  • the optical read surface is large.
  • the support contacts the optical read surface.
  • the contact is not continuous with the optical read surface.
  • the support spreads centrifugal forces to a larger area than the centrifugal forces for a well plate not using the support.
  • use of the support reduces deformation of the well plate overall and/or the optical read surface.
  • use of a support results in a reduction in engineering stress during centrifugation.
  • engineering stress is significantly reduced for well plates using a support during centrifugation.
  • the support acts as a cover to prevent dust from getting on the imaging surface.
  • FIG. 1 provides a line drawing of some embodiments of a well plate 1 seated on a support 2.
  • the exploded view of some embodiments, shown in FIG. 2, shows the well plate 1 and support 2 as physically distinct components.
  • the support 2 comprises of at least three tiered levels: a top level 5, a middle level 6, a lower level 7 as shown in FIG.
  • the support 2 comprises two tired levels: a top level 5 and a lower level 7
  • the length and the width of the support 2 are the same as the length and the width of the lower level 7.
  • the length of the lower level 7 is within the range of 120 mm to 140 mm.
  • the length of the lower level 7 is within a range selected from the group consisting of: 120 mm to 121 mm, 121 mm to 122 mm, 122 mm to 123 mm, 123 mm to 124 mm, 124 mm to 125 mm, 125 mm to 126 mm, 126 mm to 127 mm, 127 mm to 128 mm, 128 mm to 129 mm, 129 mm to 130 mm, 130 mm to 131 mm, 131 mm to 132 mm, 132 mm to 133 mm, 133 mm to 134 mm, 134 mm to 135 mm, 135 mm to 136 mm, 136 mm to 137 mm, 137 mm to 138 mm, 138 mm to 139 mm, and 139 mm to 140 mm.
  • the length of the lower level 7 is 127.9 mm.
  • the width of the lower level 7 is within the range of 75 mm to 95 mm. In some embodiments, the width of the lower level 7 is within a range selected from the group consisting of: 75 mm to 76 mm, 76 mm to 77 mm, 77 mm to 78 mm, 78 mm to 79 mm, 79 mm to 80 mm, 80 mm to 81 mm, 81 mm to 82 mm, 82 mm to 83 mm, 83 mm to 84 mm, 84 mm to 85 mm, 85 mm to 86 mm, 86 mm to 87 mm, 87 mm to 88 mm, 88 mm to 89 mm, 89 mm to 90 mm, 90 mm to 91 mm, 91 mm to 92 mm, 92 mm to 93 mm, 93 mm to 94 mm, and 94 mm to 95 mm. In some embodiments, the width of
  • the length of the middle level 6 is less than the length of the lower level 7. In some embodiments, the length of the middle level 6 is within a range of 115 mm to 135 mm. In some embodiments, the length of the lower level 7 is within a range selected from the group consisting of: 115 mm to 116 mm, 116 mm to 117 mm, 1 18 mm to 119 mm, 119 mm to 120 mm, 102 mm to 121 mm, 121 mm to 122 mm, 122 mm to 123 mm, 123 mm to 124 mm, 124 mm to 125 mm, 125 mm to 126 mm, 126 mm to 127 mm, 127 mm to 128 mm, 128 mm to 129 mm, 129 mm to 130 mm, 130 mm to 131 mm, 131 mm to 132 mm, 132 mm to 133 mm, 133 mm to 134 mm
  • the width of the middle level 6 is less than the width of the lower level 7. In some embodiments, the width of the middle level 6 is within a range of 70 mm to 90 mm. In some embodiments, the width of the middle level 6 is within a range selected from the group consisting of: 70 mm to 71 mm, 71 mm to 72 mm, 72 mm to 73 mm, 73 mm to 74 mm, 74 mm to 75 mm, 75 mm to 76 mm, 76 mm to 77 mm, 77 mm to 78 mm, 78 mm to 79 mm, 79 mm to 80 mm, 80 mm to 81 mm, 81 mm to 82 mm, 82 mm to 83 mm, 83 mm to 84 mm, 84 mm to 85 mm, 85 mm to 86 mm, 86 mm to 87 mm, 87 mm to 88 mm, 88 mm to 89
  • At least one of the middle level 6 and the lower level 7 guide the well plate 1 into place over the support 2. In some embodiments, at least one of the middle level 6 and the lower level 7 prevent the well plate 1 from slipping off the support 2 during centrifugation or handling.
  • the length of the top level 5 is less than the length of the middle level 6. In some embodiments, the length of the top level 5 is less than the length of the lower level 7. In some embodiments, the length of the top level 5 is within the range selected from 105 mm to 130 mm.
  • the length of the lower level 7 is within a range selected from the group consisting of: 105 mm to 106 mm, 106 mm to 107 mm, 107 mm to 108 mm, 108 mm to 109 mm, 109 mm to 110 mm, 110 mm to 11 1 mm, 111 mm to 112 mm, 112 mm to 113 mm, 113 mm to 114 mm, 114 mm to 115 mm, 115 mm to 1 16 mm, 116 mm to 117 mm, 118 mm to 119 mm, 119 mm to 120 mm, 121 mm to 122 mm, 122 mm to 123 mm, 123 mm to 124 mm, 124 mm to 125 mm, 125 mm to 126 mm, 126 mm to 127 mm, 127 mm to 128 mm, 128 mm to 129 mm, 129 mm to 130 mm.
  • the width of the top level 5 is less than the length of the middle level 6. In some embodiments, the width of the top level 5 is less than the length of the lower level 7. In some embodiments, the length of the top level 5 is within the range selected from 65 mm to 85 mm.
  • the width of the lower level 7 is within a range selected from the group consisting of: 65 mm to 66 mm, 66 mm to 67 mm, 67 mm to 68 mm, 68 mm to 69 mm, 69 mm to 70 mm, 70 mm to 71 mm, 71 mm to 72 mm, 72 mm to 73 mm, 73 mm to 74 mm, 74 mm to 75 mm, 75 mm to 76 mm, 76 mm to 77 mm, 77 mm to 78 mm, 78 mm to 79 mm, 79 mm to 80 mm, 80 mm to 81 mm, 81 mm to 82 mm, 82 mm to 83 mm, 83 mm to 84 mm, and 84 mm to 85 mm.
  • the length of the top level 5 is 79.5 mm.
  • the support 2 physically contacts the well plate 1.
  • the support 2 physically contacts the optical read surface 3 of the well plate 1.
  • other surfaces 4 are not in contact with the support 2 and an open space exists between the support 2 and the well plate 1 to ensure the optical surface 3 is always supported by the top level 5 of the support 2.
  • the other surfaces 4 comprise the lower level 7 and the middle level 6.
  • the lower level 7 and the middle level 6 do not physically contact the well plate 1 .
  • the support 2 does not physically contact the side of the top level 5.
  • the support 2 does not physically contact the side or the top of the middle level 6.
  • the support 2 does not physically contact the top of the lower level 7.
  • the lack of physical contact described above prevent over-constraint of the well plate 1.
  • the lack of physical contact results in empty space between the other surfaces 4 and the well plate 1 .
  • an empty space exists between the side of the top level 5 and the well plate 1.
  • an empty space exists between the side of the middle level 6 and the well plate 1.
  • an empty space exists between the top of the middle level 6 and the well plate 1 .
  • an empty space exists between the top of the lower level 7 and the well plate 1.
  • the support 2 enables a well plate 1 to be centrifuged with much lower risk of failure due to bending and stresses experienced during the centrifugation process compared to the risk during centrifugation of a well plate 1 without the support 2.
  • the support 2 is single-use. In some embodiments, the support 2 is multi-use. The design of the support 2 may be adjusted for the specific well plate 1 to be supported. In some embodiments, the support 2 is produced by any means appropriate. In some embodiments, the support 2 is produced by machining. In some embodiments, the support 2 is produced by injection-molding. In some embodiments, the support 2 is produced by forming. In some embodiments, the support 2 is produced by three-dimensional (3D) printing.
  • the support 2 is made of any appropriate material.
  • the support 2 is made of polyethylene terephthalate glycol (PETG) plastic.
  • the support 2 is made of polypropylene (PP) homopolymer plastic.
  • the support 2 is made of polystyrene (PS).
  • the support 2 is made of acrylonitrile butadiene styrene (ABS).
  • a support 2 designed to prevent deformation of a well plate 1 used in laboratory techniques requiring high centrifugation forces.
  • the support 2 is used during Millipore® (single molecule counting) SMC® Assays.
  • an eluted product is pipetted into a well plate such as Greiner #781892 or Aurora #ABB200160A.
  • the plate is centrifuged the plate at 1000 x gravity to ensure that the reagent product is at the bottom of the well and there are no air bubbles.
  • Such centrifugation can cause failure in well plates, especially high resolution imaging well plates including an optical read surface that is glass, such as Greiner #781892.
  • the optical read surface is made of transparent plastic.
  • All ranges for formulations recited herein include ranges therebetween and can be inclusive or exclusive of the endpoints.
  • Optional included ranges are from integer values therebetween (or inclusive of one original endpoint), at the order of magnitude recited or the next smaller order of magnitude.
  • the lower range value is 0.2
  • optional included endpoints can be 0.3, 0.4,... 1.1 , 1.2, and the like, as well as 1 , 2, 3 and the like; if the higher range is 8, optional included endpoints can be 7, 6, and the like, as well as 7.9, 7.8, and the like.
  • One-sided boundaries, such as 3 or more similarly include consistent boundaries (or ranges) starting at integer values at the recited order of magnitude or one lower.
  • 3 or more includes 4, or 3.1 or more.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Optical Measuring Cells (AREA)

Abstract

La divulgation concerne un support pour une plaque à puits, le support comprenant : une structure rectangulaire étagée comprenant au moins un niveau supérieur et un niveau inférieur ; une longueur et une largeur du niveau supérieur étant inférieures à une longueur et à une largeur du niveau inférieur, et la longueur et la largeur du niveau supérieur étant au moins identiques à une longueur et à une largeur d'une surface de lecture optique de la plaque à puits ; et la longueur et la largeur du niveau inférieur étant supérieures à la longueur et à la largeur du niveau supérieur, et la longueur et la largeur du niveau inférieur étant au moins identiques à une longueur et à une largeur de la plaque à puits. La divulgation concerne un support destiné à empêcher la déformation d'une plaque à puits pendant la centrifugation. Dans certains modes de réalisation, le support comprend des niveaux étagés pour garantir que la plaque à puits n'entre pas en contact physique en continu avec le support.
PCT/US2024/032534 2023-09-27 2024-06-05 Support de plaque à puits Pending WO2025071696A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363585696P 2023-09-27 2023-09-27
US63/585,696 2023-09-27

Publications (1)

Publication Number Publication Date
WO2025071696A1 true WO2025071696A1 (fr) 2025-04-03

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ID=91664520

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/032534 Pending WO2025071696A1 (fr) 2023-09-27 2024-06-05 Support de plaque à puits

Country Status (1)

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WO (1) WO2025071696A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1395645B1 (fr) * 2001-06-14 2013-05-22 EMD Millipore Corporation Trous d'acces de plaque de filtre multipuits pour appareil d'essai multipuits
US8602958B1 (en) * 2007-11-29 2013-12-10 Life Technologies Corporation Methods and assemblies for collecting liquid by centrifugation
EP2929939A1 (fr) * 2014-04-07 2015-10-14 Yantai AusBio Laboratories Co., Ltd. Microplaque
US11413619B1 (en) * 2020-07-17 2022-08-16 Lidong Qin Double-layer multi-well plate

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1395645B1 (fr) * 2001-06-14 2013-05-22 EMD Millipore Corporation Trous d'acces de plaque de filtre multipuits pour appareil d'essai multipuits
US8602958B1 (en) * 2007-11-29 2013-12-10 Life Technologies Corporation Methods and assemblies for collecting liquid by centrifugation
EP2929939A1 (fr) * 2014-04-07 2015-10-14 Yantai AusBio Laboratories Co., Ltd. Microplaque
US11413619B1 (en) * 2020-07-17 2022-08-16 Lidong Qin Double-layer multi-well plate

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