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WO2025066469A1 - Cartouche de réactifs et appareil d'analyse d'échantillons - Google Patents

Cartouche de réactifs et appareil d'analyse d'échantillons Download PDF

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Publication number
WO2025066469A1
WO2025066469A1 PCT/CN2024/106530 CN2024106530W WO2025066469A1 WO 2025066469 A1 WO2025066469 A1 WO 2025066469A1 CN 2024106530 W CN2024106530 W CN 2024106530W WO 2025066469 A1 WO2025066469 A1 WO 2025066469A1
Authority
WO
WIPO (PCT)
Prior art keywords
chamber
flow path
valve stem
valve
driving
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/CN2024/106530
Other languages
English (en)
Chinese (zh)
Inventor
黄宏坤
李晓峰
黄超杰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fapon Biotech Inc
Guangdong Runpon Bioscience Co Ltd
Original Assignee
Fapon Biotech Inc
Guangdong Runpon Bioscience Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fapon Biotech Inc, Guangdong Runpon Bioscience Co Ltd filed Critical Fapon Biotech Inc
Publication of WO2025066469A1 publication Critical patent/WO2025066469A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Definitions

  • the present disclosure relates to the technical field of nucleic acid detection, and in particular to a reagent cartridge and a sample detection device.
  • the present disclosure provides a reagent cartridge and a sample detection device, which can conveniently and accurately control the on-off of a liquid channel, thereby realizing the transfer of liquid between different chambers, and simplifies the structure of the reagent cartridge and reduces production costs.
  • An embodiment of the present disclosure provides a reagent cartridge, comprising:
  • the box body includes a driving chamber, a rotary valve, an elution chamber and a plurality of accommodating chambers, and the driving chamber, the rotary valve, the elution chamber and the plurality of accommodating chambers are sequentially connected and integrated into one body;
  • reaction tube the reaction tube is connected to the driving chamber, and the reaction tube is communicated with the driving chamber;
  • a first flow path is provided on the side wall of the elution cavity, and a second flow path is provided on the side wall of the driving cavity;
  • the rotary valve is configured to connect or block the first flow path and the second flow path during rotation;
  • a driving member which is movably disposed in the driving chamber.
  • the driving member is configured to form a negative pressure in the driving chamber and the reaction tube during movement relative to the driving chamber when the first flow path and the second flow path are connected, so that the liquid in the elution chamber enters the reaction tube through the second flow path, the rotary valve, and the first flow path in sequence.
  • the rotary valve includes a valve cavity, a valve stem and a swinging member, the upper portion of the valve stem is connected to the swinging member, a through hole is provided on the valve stem, the lower portion of the valve stem is located in the valve cavity, and the swinging member is configured to rotate the valve stem so that the valve stem can rotate relative to the valve cavity;
  • the cavity openings of the valve cavity, the driving cavity, the elution cavity and the plurality of accommodating cavities are all located on the upper end surface of the box body.
  • a first limit block and a second limit block are provided on the valve chamber along the rotation path of the swing member, and the first limit block and the second limit block are configured to limit the swing stroke of the swing member.
  • the first flow path is connected to the second flow path.
  • the rotary valve when the swinging member rotates to the first limit block, the rotary valve is in the first position, and the through hole connects the first flow path to the second flow path;
  • the rotary valve When the swinging member is located between the first limit block and the second limit block and does not contact the first limit block, the rotary valve is in the second position, and the rotary valve blocks the first flow path and the second flow path.
  • valve stem includes a first valve stem portion, a second valve stem portion and a connecting portion, one end of the first valve stem portion is connected to the swinging member, the other end of the first valve stem portion is connected to one end of the second valve stem portion, and the connecting portion is connected to the other end of the second valve stem portion;
  • the first valve stem portion comprises two cross-intersecting plate-shaped structures, and the second valve stem portion is provided with a through hole.
  • the first valve stem portion is provided with annular protrusions at intervals along the extension direction of the first valve stem portion, and the annular protrusions are configured to be in close contact with the valve cavity to prevent the first valve stem portion and the second valve stem portion from tilting during rotation.
  • the connecting portion includes a plurality of claws, and for each claw, one end of the claw is connected to the second valve stem portion, and the other end of the claw is provided with a limiting protrusion; the plurality of claws are evenly distributed along the circumferential direction of the second valve stem, and there is a gap between two adjacent claws, the plurality of claws are made of elastic material, and the plurality of claws are retractable;
  • a protrusion is arranged in the valve cavity.
  • the multiple claws shrink inwardly; when the limiting protrusion passes through the protruding position, the multiple claws rebound, thereby realizing the fixed limiting of the rotary valve and the valve cavity.
  • one end of the claw away from the valve stem portion is a guiding arc surface.
  • the second flow path includes a horizontal channel and a vertical channel, the horizontal channel is located at the same horizontal plane as the first flow path, and the vertical channel is configured to connect the reaction tube and the horizontal channel.
  • a blocking member is provided at one end of the horizontal channel away from the rotary valve.
  • the multiple accommodating chambers are a first cleaning chamber, a second cleaning chamber, a magnetic bead chamber, a sample chamber and a magnetic rod sleeve chamber, and the first cleaning chamber, the second cleaning chamber, the magnetic bead chamber, the sample chamber and the magnetic rod sleeve chamber are arranged in sequence from one end of the box body close to the elution chamber to the end of the box body away from the elution chamber.
  • shaped holes are provided at radial positions of partial flow sections of the first flow path and the second flow path.
  • An embodiment of the present disclosure further provides a sample detection device, comprising a detection component and any of the above-mentioned reagent cartridges, wherein the reagent cartridge is connected to the detection component.
  • the detection assembly includes a detection platform, a motion mechanism, a magnetic rod sleeve grasping mechanism and a toggle member, and the detection platform is configured to place a reagent cartridge;
  • the motion mechanism is connected to the magnetic rod cover grabbing mechanism and the toggle member respectively, and the motion mechanism is located above the detection platform, and the motion mechanism drives the magnetic rod cover grabbing mechanism and the toggle member to move above the reagent cartridge;
  • the magnetic rod sleeve grabbing mechanism is configured to grab the magnetic rod for sample testing;
  • the toggle member is configured to rotate the rotary valve;
  • the motion mechanism is provided with a groove, and the groove is configured to clamp the driving member.
  • the motion mechanism moves up and down, so that the driving member moves relative to the driving cavity.
  • the detection assembly further includes a movable member, and the movable member is configured to drive the driving member to move relative to the driving cavity.
  • the transfer of liquid between different chambers is realized, so that the connection and disconnection between the first flow path and the second flow path can be conveniently and accurately controlled.
  • multiple cavities are opened on the box body, and the rotary valve that controls the connection and blocking of the first flow path in the elution cavity and the second flow path in the driving cavity is set in one of the cavities of the box body, which simplifies the structure of the reagent cartridge and reduces the production cost.
  • the driving member and the rotary valve are both located above the box body, which is convenient for setting a moving mechanism above the reagent cartridge to drive the driving member and the rotary valve to move, and is convenient for the integration of the sample detection device.
  • FIG1 is a schematic diagram of a sample detection device provided in an embodiment of the present disclosure.
  • FIG2 is a schematic diagram of a reagent cartridge provided in an embodiment of the present disclosure from a first viewing angle
  • FIG3 is a schematic diagram of a reagent cartridge provided in an embodiment of the present disclosure from a second viewing angle
  • FIG4 is a schematic diagram of the A-A section provided in an embodiment of the present disclosure.
  • FIG5 is a schematic diagram of a BB cross section provided in an embodiment of the present disclosure.
  • FIG. 6 is a schematic diagram of a rotary valve provided in an embodiment of the present disclosure.
  • Icons 1-sample detection device; 1000-reagent cartridge; 100-cartridge body; 110-driving chamber; 111-second flow path; 1111-horizontal channel; 1112-vertical channel; 120-rotary valve; 121-valve chamber; 1211-first limit block; 1212-second limit block; 1213-protrusion; 122-valve stem; 1221-first valve stem portion; 12211-annular protrusion; 1222-second valve stem portion; 12221-through hole; 12222-annular groove; 1223-connecting portion; 12231-claw ;122311-limiting protrusion;122312-guiding arc surface;123-swinging member;130-elution chamber;131-first flow path;140-accommodating chamber;141-first cleaning chamber;142-second cleaning chamber;143-magnetic bead chamber;144-sample chamber;145-magnetic rod sleeve chamber;200-reaction tube;300-driving member;400-sealing member;
  • a reagent cartridge 1000 and a sample detection device 1 provided in the embodiments of the present disclosure can effectively improve the above-mentioned technical problems.
  • the reagent cartridge 1000 can conveniently and accurately control the on-off of the liquid path, thereby realizing the transfer of liquid between different chambers, and simplifies the structure of the reagent cartridge 1000, reducing the production cost.
  • the reagent cartridge 1000 is applied to the sample detection device 1.
  • the driving member 300 and the rotary valve 120 of the reagent cartridge 1000 are both located above the box body 100.
  • the use of the reagent cartridge 1000 facilitates the installation of a motion mechanism 2200 above the reagent cartridge 1000 to drive the driving member 300 and the rotary valve 120 to move, thereby facilitating the integration of the sample detection device 1.
  • FIG1 is a schematic diagram of a sample detection device 1 provided in an embodiment of the present disclosure.
  • the sample detection device 1 provided in an embodiment of the present disclosure includes a detection component 2000 and a reagent cartridge 1000 , and the reagent cartridge 1000 is connected to the detection component 2000 .
  • the detection component 2000 provided in this embodiment includes a detection platform 2100, a motion mechanism 2200, a magnetic rod sleeve grasping mechanism 2300 and a toggle member 2400, the detection platform 2100 is configured to place the reagent cartridge 1000; the motion mechanism 2200 is connected to the magnetic rod sleeve grasping mechanism 2300 and the toggle member 2400 respectively, the motion mechanism 2200 is located above the detection platform 2100, and the motion mechanism 2200 drives the magnetic rod sleeve grasping mechanism 2300 and the toggle member 2400 to move above the reagent cartridge 1000; the magnetic rod sleeve grasping mechanism 2300 is configured to grasp the magnetic rod sleeve for sample detection; the toggle member 2400 is configured to rotate the rotary valve 120; the motion mechanism 2200 is provided with a groove 2210, the groove 2210 is configured to clamp the driving member 300, and the driving member 300 moves relative to the driving chamber 110 through the up and down movement of the motion mechanism 2200.
  • the magnetic rod sleeve is configured to adsorb
  • the moving mechanism 2200 is provided with a groove 2210, and the groove 2210 is configured to clamp the driving member 300, and the moving mechanism 2200 moves up and down, so that the driving member 300 moves relative to the driving chamber 110.
  • the magnetic rod sleeve is configured to adsorb magnetic beads and mix the sample liquid for sample detection
  • the driving member 300 is a piston
  • the moving mechanism 2200 moves along the X direction so that the groove 2210 is located obliquely above the driving member 300, and then the moving mechanism 2200 moves along the Z direction in a direction close to the detection platform 2100, so that the groove 2210 and the upper part of the piston are located in the same horizontal plane; then the moving mechanism 2200 moves along the X direction so that the groove 2210 is clamped with the piston, and the moving mechanism 2200 moves upward along the Z direction away from the detection platform 2100 to lift the piston upward.
  • the moving mechanism 2200 puts the piston back into the driving chamber 110 downward, and then the
  • the detection assembly 2000 may also include a movable member (not shown), the movable member replaces the above-mentioned groove 2210 structure, and the movable member is configured to drive the driving member 300 so that the driving member 300 moves relative to the driving chamber 110.
  • the mode in which the movable member drives the driving member 300 is similar to the mode of motion of the above-mentioned groove 2210 structure, and will not be described in detail here.
  • a connecting structure is provided on the reagent cartridge 1000, and the reagent cartridge 1000 is fixedly connected to the detection platform 2100 through the connecting structure.
  • the motion mechanism 2200 above the detection platform 2100 can drive the magnetic rod cover grasping mechanism 2300, the toggle member 2400 and the movable member to move along the three directions of X, Y, and Z, so that it moves above the reagent cartridge 1000 to complete the sample detection work.
  • the motion mechanism 2200 mainly moves along the X and Z directions.
  • Multiple reagent cartridges 1000 placement positions can be set on the detection platform 2100, and multiple grooves 2210 can be opened on the motion mechanism 2200 to connect multiple magnetic rod sleeve grasping mechanisms 2300 and multiple toggle members 2400.
  • the spacing distances between the multiple grooves 2210, the multiple magnetic rod sleeve grasping mechanisms 2300, and the multiple toggle members 2400 are the same as the spacing distances between the multiple reagent cartridges 1000.
  • the structure of the reagent cartridge 1000 is described in detail below.
  • Figure 2 is a schematic diagram of the reagent cartridge 1000 provided in the embodiment of the present disclosure from a first perspective
  • Figure 3 is a schematic diagram of Figure 2 from another perspective
  • Figure 4 is a schematic diagram of the A-A section provided in the embodiment of the present disclosure
  • Figure 5 is a schematic diagram of the B-B section provided in the embodiment of the present disclosure.
  • the reagent cartridge 1000 provided in the embodiment of the present disclosure includes a cartridge body 100, a reaction tube 200 and a driving member 300.
  • the cartridge body 100 includes a driving chamber 110, a rotary valve 120, an elution chamber 130 and a plurality of accommodating chambers 140.
  • the driving chamber 110, the rotary valve 120, the elution chamber 130 and the plurality of accommodating chambers 140 are connected in sequence.
  • the reaction tube 200 and the driving chamber 110 are connected, and the reaction tube 200 and the driving chamber 110 are connected;
  • a first flow path 131 is provided on the side wall of the elution chamber 130, and a second flow path 111 is provided on the side wall of the driving chamber 110;
  • the rotary valve 120 is configured to connect or block the first flow path 131 and the second flow path 111 during the rotation process;
  • the driving member 300 is movably arranged in the driving chamber 110, and the driving member 300 is configured to form a negative pressure in the driving chamber 110 and the reaction tube 200 during the movement relative to the driving chamber 110 when the first flow path 131 and the second flow path 111 are connected, so that the liquid in the elution chamber 130 enters the reaction tube 200 through the second flow path 111, the rotary valve 120, and the first flow path 131 in sequence.
  • the rotary valve 120 can more conveniently control the connection and blocking of the first flow path 131 and the second flow path 111.
  • the rotary valve 120 is arranged in one of the cavities of the box body 100 to simplify the structure of the reagent cartridge 1000.
  • FIG. 6 is a schematic diagram of a rotary valve 120 provided in an embodiment of the present disclosure.
  • the driving member 300 provided in this embodiment is a piston.
  • the rotary valve 120 in this embodiment includes The valve cavity 121, the valve stem 122 and the swinging member 123, the upper part of the valve stem 122 is connected to the swinging member 123, the valve stem 122 is provided with a through hole 12221, the lower part of the valve stem 122 is located in the valve cavity 121, and the swinging member 123 is configured to rotate the valve stem 122, so that the valve stem 122 can rotate relative to the valve cavity 121.
  • the cavity openings of the valve cavity 121 and the driving cavity 110, the elution cavity 130 and the plurality of accommodating cavities 140 are all located on the upper end surface of the box body 100.
  • the swinging member 123 in this embodiment includes a body and a rectangular convex strip, the toggle member 2400 contacts the convex strip, and the toggle member 2400 drives the swinging member 123 to rotate by its own rotation, thereby driving the valve stem 122 to rotate relative to the valve cavity 121.
  • the toggle member 2400 rotates the swing member 123 and then drives the valve stem 122 to rotate relative to the valve chamber 121, so that the through hole 12221 connects the first flow path 131 and the second flow path 111
  • the movable member drives the piston to move upward relative to the drive chamber 110
  • the reaction tube 200 is connected with the drive chamber 110
  • a negative pressure is formed in the drive chamber 110, so that the liquid in the elution chamber 130 passes through the second flow path 111, the through hole 12221 of the rotary valve 120 and the first flow path 131 and enters the reaction tube 200.
  • the drive member 300 can also be other structures that can move relative to the drive chamber 110, which is not limited here.
  • the shape of the convex strip is also not limited, as long as it is convenient for the toggle member 2400 to drive it to rotate.
  • the moving mechanism 2200 drives the toggle member 2400 to move along the X direction.
  • the through hole 12221 can also be connected to the first flow path 131 and the second flow path 111, so as to rotate the convex strip and drive the valve stem 122 to rotate relative to the valve cavity 121.
  • the rotary valve 120 can be controlled to connect the first flow path 131 and the second flow path 111 without precisely controlling the rotation angle.
  • the valve chamber 121 is provided with a first limit block 1211 and a second limit block 1212 along the rotation path of the swing member 123.
  • the first limit block 1211 and the second limit block 1212 are configured to limit the swing stroke of the swing member 123.
  • the swing member 123 is located at the position of the first limit block 1211, the first flow path 131 and the second flow path 111 are connected.
  • the rotary valve 120 When the swing member 123 rotates to the first limit block 1211, the rotary valve 120 is in the first position; when the swing member 123 is located between the first limit block 1211 and the second limit block 1212 and does not contact the first limit block 1211, the rotary valve 120 is in the second position.
  • the through hole 12221 connects the first flow path 131 and the second flow path 111 ; when the rotary valve 120 is in the second position, the rotary valve 120 blocks the first flow path 131 and the second flow path 111 .
  • the second flow path 111 in this embodiment includes a horizontal channel 1111 and a vertical channel 1112.
  • the horizontal channel 1111 and the first flow path 131 are located at the same horizontal plane, and the vertical channel 1112 is configured to connect the reaction tube 200 and the horizontal channel 1111.
  • a blocking member 400 is provided at one end of the horizontal channel 1111 away from the rotary valve 120.
  • the valve stem 122 provided in the invention includes a first valve stem portion 1221, a second valve stem portion 1222 and a connecting portion 1223, one end of the first valve stem portion 1221 is connected to the swinging member 123, the other end of the first valve stem portion 1221 is connected to one end of the second valve stem portion 1222, and the connecting portion 1223 is connected to the other end of the second valve stem portion 1222.
  • the first valve stem portion 1221 includes two cross-intersecting plate structures, and the second valve stem portion 1222 is provided with a through hole 12221.
  • the second valve stem portion 1222 is provided with an annular groove 12222, and an annular sealing gasket is embedded in the annular groove 12222.
  • the first valve stem portion 1221 in this embodiment is provided with annular protrusions 12211 at intervals along the extension direction of the first valve stem portion 1221, and the annular protrusions 12211 are configured to be in close contact with the valve cavity 121 to prevent the first valve stem portion 1221 and the second valve stem portion 1222 from tilting during rotation.
  • the connecting portion 1223 provided in the embodiment of the present disclosure includes a plurality of claws 12231.
  • one end of the claw 12231 is connected to the second valve stem portion 1222, and the other end of the claw 12231 is provided with a limiting protrusion 122311; the plurality of claws 12231 are evenly distributed along the circumferential direction of the second valve stem 122, and there is a gap between two adjacent claws 12231.
  • the plurality of claws 12231 are all made of elastic material, and the plurality of claws 12231 can shrink inward.
  • a protrusion 1213 is provided in the valve cavity 121.
  • the limiting protrusion 122311 passes through the position of the protrusion 1213, the plurality of claws 12231 shrink inwardly; when the limiting protrusion 122311 passes through the position of the protrusion 1213, the plurality of claws 12231 rebound, thereby achieving fixed limiting of the valve stem 122 and the valve cavity 121, thereby achieving installation of the rotary valve 120.
  • the protrusion 1213 on the valve cavity 121 can be provided with a connecting portion 1223 to ensure that the through hole 12221 and the first flow path 131 and the second flow path 111 are located in the same horizontal plane, which is convenient for installation.
  • the end of the claw 12231 away from the second valve stem portion 1222 is a guide arc surface 122312.
  • the number of the claws 12231 is four, and the four claws 12231 are evenly distributed along the circumferential direction of the second valve stem 122, and there is a gap between two adjacent claws 12231.
  • the number of the claws 12231 can also be two, three, etc., which is not limited here.
  • the multiple accommodating chambers 140 provided in the embodiment of the present disclosure are a first cleaning chamber 141, a second cleaning chamber 142, a magnetic bead chamber 143, a sample chamber 144, and a magnetic rod sleeve chamber 145.
  • the first cleaning chamber 141, the second cleaning chamber 142, the magnetic bead chamber 143, the sample chamber 144, and the magnetic rod sleeve chamber 145 are sequentially arranged from the end of the box body 100 close to the elution chamber 130 to the end of the box body 100 away from the elution chamber 130.
  • one or more cleaning chambers can be added between the first cleaning chamber 141 and the second cleaning chamber 142.
  • the magnetic rod sleeve chamber is configured to carry the magnetic rod sleeve assembly
  • the sample chamber is configured to hold the sample solvent
  • the magnetic bead chamber is configured to hold the magnetic bead preservation solution
  • the magnetic bead preservation solution contains magnetic beads
  • the cleaning chamber is configured to hold the washing solution
  • the first elution chamber and the second elution chamber are both configured to hold the elution solution.
  • the sample solvent is first injected into the sample chamber.
  • the magnetic rod sleeve grabbing mechanism 2300 holds the magnetic rod sleeve assembly
  • the moving mechanism 2200 then drives the toggle member 2400 to toggle the swing member 123 of the rotary valve 120, so that the through hole 12221 of the rotary valve 120 is connected to the first flow path 131 and the second flow path 111.
  • the moving member drives the piston to move upward relative to the driving chamber 110, and the reaction tube 200 is connected to the driving chamber 110, and a negative pressure is formed in the driving chamber 110, so that the liquid in the elution chamber 130 passes through the second flow path 111, the through hole 12221 of the rotary valve 120 and the first flow path 131 to enter the reaction tube 200, and finally the detection module detects and analyzes the reagent in the reaction tube 200.
  • the reagent cartridge 1000 includes a cartridge body 100, a reaction tube 200 and a driving member 300.
  • the cartridge body 100 includes a driving chamber 110, a rotary valve 120, an elution chamber 130 and a plurality of accommodating chambers 140.
  • the driving chamber 110, the rotary valve 120, the elution chamber 130 and the plurality of accommodating chambers 140 are sequentially connected and integrated into one body.
  • the reaction tube 200 is connected to the driving chamber 110, and the reaction tube 200 is in communication with the driving chamber 110.
  • a first flow path 131 is provided on the side wall of the elution chamber 130, and a second flow path 132 is provided on the side wall of the driving chamber 110.
  • the rotary valve 120 is configured to connect or block the first flow path 131 and the second flow path 111 during rotation;
  • the driving member 300 can be movably arranged in the driving chamber 110, and the driving member 300 is configured to form a negative pressure in the driving chamber 110 and the reaction tube 200 during the movement relative to the driving chamber 110 when the first flow path 131 and the second flow path 111 are connected, so that the liquid in the elution chamber 130 enters the reaction tube 200 through the second flow path 111, the rotary valve 120, and the first flow path 131 in sequence.
  • the rotary valve 120 By using the rotary valve 120 to control the on-off between the first flow path 131 and the second flow path 111, the transfer of liquid between different chambers is realized, so that the on-off between the first flow path 131 and the second flow path 111 can be conveniently and accurately controlled.
  • a plurality of cavities are provided on the box body 100, and the rotary valve 120 for controlling the connection and blocking of the first flow path 131 in the elution chamber 130 and the second flow path 111 in the driving chamber 110 is set as one of the cavities of the box body 100, which simplifies the structure of the reagent cartridge 1000 and reduces the production cost.
  • the driving member 300 and the rotary valve 120 are both located above the box body 100, so that a moving mechanism is provided above the reagent cartridge 1000 to drive the driving member 300 and the rotary valve 120 to move, which is convenient for the integration of the sample detection device 1.
  • the reagent cartridge disclosed in the present invention can be used in a sample detection device, and the reagent cartridge uses a rotary valve to control the on-off between the first flow path and the second flow path to achieve the transfer of liquid between different chambers, so that it can conveniently and accurately control the flow of liquid between the first flow path and the second flow path.
  • a plurality of cavities are provided on the box body, and a rotary valve for controlling the connection and blocking of the first flow path in the elution cavity and the second flow path in the driving cavity is provided in one of the cavities of the box body, which simplifies the structure of the reagent cartridge and reduces the production cost.
  • the driving member and the rotary valve are both located above the box body, which facilitates the provision of a moving mechanism above the reagent cartridge to drive the driving member and the rotary valve to move, and facilitates the integration of the sample detection device.

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Abstract

Cartouche de réactifs et appareil d'analyse d'échantillons, relatifs au domaine technique de l'analyse de l'acide nucléique. La cartouche de réactifs comporte les éléments suivants : un corps de cartouche, un tube de réaction et un élément d'entraînement. Le corps de cartouche comporte une chambre d'entraînement, une vanne rotative, une chambre d'élution et de multiples chambres d'accueil, la chambre d'entraînement et la vanne rotative, la chambre d'élution et les multiples chambres d'accueil étant connectées en séquence et intégrées dans un corps ; le tube de réaction est connecté à la chambre d'entraînement, et le tube de réaction est en communication avec la chambre d'entraînement ; une première voie d'écoulement est contenue dans une paroi latérale de la chambre d'élution, et une deuxième voie d'écoulement est contenue dans une paroi latérale de la chambre d'entraînement ; la vanne rotative est conçue pour ouvrir la première voie d'écoulement et la deuxième voie d'écoulement pendant la rotation de la vanne ; l'élément moteur est agencé de manière mobile dans la chambre d'entraînement, l'élément moteur étant conçu pour constituer une pression négative dans la chambre d'entraînement et le tube de réaction pendant le mouvement par rapport à la chambre d'entraînement lorsque la première voie d'écoulement et la deuxième voie d'écoulement sont en communication, de manière à ce qu'un liquide dans la chambre d'élution pénètre dans le tube de réaction par l'intermédiaire, successivement, de la deuxième voie d'écoulement, de la vanne rotative et de la première voie d'écoulement.
PCT/CN2024/106530 2023-09-25 2024-07-19 Cartouche de réactifs et appareil d'analyse d'échantillons Pending WO2025066469A1 (fr)

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