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WO2025065564A1 - Systèmes et procédés de coloration d'immunohistochimie (ihc) à commande dynamique - Google Patents

Systèmes et procédés de coloration d'immunohistochimie (ihc) à commande dynamique Download PDF

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Publication number
WO2025065564A1
WO2025065564A1 PCT/CN2023/122724 CN2023122724W WO2025065564A1 WO 2025065564 A1 WO2025065564 A1 WO 2025065564A1 CN 2023122724 W CN2023122724 W CN 2023122724W WO 2025065564 A1 WO2025065564 A1 WO 2025065564A1
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WO
WIPO (PCT)
Prior art keywords
staining
heating chamber
hier
ihc
formation
Prior art date
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Pending
Application number
PCT/CN2023/122724
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English (en)
Inventor
Jee Jong Shum
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Shenzhen Prs Ltd
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Shenzhen Prs Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenzhen Prs Ltd filed Critical Shenzhen Prs Ltd
Priority to PCT/CN2023/122724 priority Critical patent/WO2025065564A1/fr
Publication of WO2025065564A1 publication Critical patent/WO2025065564A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates

Definitions

  • This description relates to immunohistochemistry (IHC) staining and associated systems, devices, methods, and apparatuses.
  • IHC immunohistochemistry
  • HIER heat induced epitope retrieval
  • a Legend 6G Stainer includes a dynamically controlled external air pressurized heating chamber designed for a Heat Induced Epitope Retrieval (HIER) immunohistochemistry (IHC) staining process, said heating chamber being of low mass and capable of being pressurized by external air.
  • the Legend 6G Stainer further includes an adjustable air pressure control mechanism that regulates the pressurization of the heating chamber.
  • the Legend 6G Stainer further includes a capillary gap cover that protects a tissue during the HIER IHC staining process to ensure even heat distribution within a buffer pool.
  • the Legend 6G Stainer further includes a submersion system that submerges a slide within the buffer pool to eliminate formation of hot spots and micro nucleated boiling, the heating chamber and associated components allowing users to adjust a pressure within a range of 8 to 12 psi, thereby providing control over a boiling point of the HIER IHC staining process for different tissue types and staining protocol requirements.
  • a staining system includes a dynamically controlled external air pressurized heating chamber designed for a Heat Induced Epitope Retrieval (HIER) immunohistochemistry (IHC) staining process, said heating chamber being of low mass and capable of being pressurized by external air.
  • the staining system further includes an adjustable air pressure control mechanism configured to regulate the pressurization of the heating chamber.
  • the staining system further includes a capillary gap cover that protects a tissue during the HIER IHC staining process to ensure even heat distribution within a buffer pool.
  • the staining system further includes a submersion system that submerges a slide within the buffer pool to eliminate formation of hot spots and micro nucleated boiling, the heating chamber and associated components allowing users to adjust a pressure within a range of 8 to 12 psi, thereby providing control over a boiling point of the HIER IHC staining process for different tissue types and staining protocol requirements.
  • a staining method comprising dynamically controlling an external air pressurized heating chamber designed for a Heat Induced Epitope Retrieval (HIER) immunohistochemistry (IHC) staining process, said heating chamber being of low mass and capable of being pressurized by external air, regulating the pressurization of the heating chamber using an adjustable air pressure control mechanism, ensuring even heat distribution within a buffer pool using a capillary gap cover that protects a tissue during the HIER IHC staining process, submerging a slide within the buffer pool to eliminate formation of hot spots and micro nucleated boiling using a submersion system, allowing users to adjust a pressure within a range of 8 to 12 psi, thereby providing control over a boiling point of the HIER IHC staining process for different tissue types and staining protocol requirements using the heating chamber and associated components.
  • HIER Heat Induced Epitope Retrieval
  • IHC immunohistochemistry
  • Figure 1 is a table diagram of pressure ranges in relation to temperature, in accordance with some embodiments.
  • FIG. 2 is a schematic diagram of a stainer system, in accordance with some embodiments.
  • FIG. 3 is a block diagram of system architecture in accordance with some embodiments.
  • first and second features are formed in direct contact
  • additional features may be formed between the first and second features, such that the first and second features may not be in direct contact
  • present disclosure may repeat reference numerals and/or letters in the various examples. This repetition is for the purpose of simplicity and clarity and does not in itself dictate a relationship between the various embodiments and/or configurations discussed.
  • the other systems uneven heating causes bubble formation and cell cluster detachment, while in other systems an air knife displaces reagents and results in the loss of cell features.
  • the capillary gap, resembling a clam, also contributes to material loss from sectioned cells.
  • Figure 1 is a table diagram 100 of pressure ranges in relation to temperature, in accordance with some embodiments.
  • a heating chamber and associated components allow users to adjust a pressure within a pounds per square inch (psi) range, as described herein, thereby providing control over a boiling point of the HIER IHC staining process for different tissue types and staining protocol requirements.
  • psi pounds per square inch
  • a pressure range of 8 to 12 psi (102) is shown as related to particular temperatures. Therefore, dynamic control of air pressure, according to embodiments herein, allows for dynamic control of boiling point.
  • psi as a measure of pressure is merely an example and that other units are also usable for adjusting the pressure range.
  • FIG. 2 is a schematic diagram of a stainer system 200 (hereinafter referred to as “stainer system 200” ) , in accordance with some embodiments. Figure 2 is simplified for the purpose of illustration.
  • the stainer system 200 is capable of carrying out, or being used for, one or more staining processes or procedures.
  • the stainer system 200 includes a cover 202.
  • the cover 202 comprises a material suitable for forming an air-tight enclosure.
  • the cover 202 comprises a hard plastic metallic material.
  • the cover 202 comprises a plexi-glass material.
  • the cover 202 comprises a glass material.
  • the cover 202 comprises a polypropylene material.
  • the cover 202 comprises a polypropylene and polyethylene blend material.
  • the cover 202 comprises an acrylic material.
  • the cover 202 comprises another material.
  • the cover 202 comprises a dark or colored material usable to block light.
  • the stainer system 200 includes a container 204.
  • the container 204 comprises a material suitable for forming an air-tight enclosure.
  • the container 204 comprises a high temperature hard plastic material.
  • the container 204 comprises a plexi-glass metallic material.
  • the container 204 comprises a glass material.
  • the container 204 comprises a polypropylene material.
  • the container 204 comprises a polypropylene and polyethylene blend material.
  • the container 204 comprises an acrylic material.
  • the container 204 comprises another material.
  • the container 204 comprises a dark or colored material usable to block light.
  • the cover 202 and the container 204 are comprised of the same material. In some embodiments, the cover 202 and the container 204 are comprised of different materials.
  • the stainer system 200 includes a hinge 206 attached to the cover 202 and the container 204.
  • the hinge 206 allows for opening and closing of the cover 202.
  • the cover 202 and the container 204 are attached by more than one hinge or a hinge alternative.
  • the hinge 206 comprises a metallic material.
  • the hinge 206 comprises a plastic material.
  • the hinge 206 comprises a material that is the same material as the cover 202 or the container 204. In some embodiments, a hinge is not used.
  • the container 204 forms a chamber area.
  • the chamber area is a heating chamber of low mass capable of being pressurized by external air or air from sources external to the chamber area, such as an air tank compressor, etc.
  • the chamber area includes an air-circulating section or portion for circulating air.
  • the air is heated.
  • the chamber area is an air-heating area or portion.
  • the heating chamber area and associated components allow users to adjust a pressure within a psi range, such as a range of 8 to 12 psi, thereby providing control over a boiling point of the HIER IHC staining process for different tissue types and staining protocol requirements.
  • the cover 202 and the container 204 form an air-tight enclosure.
  • the cover 202 and the container 204 are capable of forming an air-tight seal.
  • the stainer system 200 includes a fan 208.
  • the fan is suitable for circulating air within a chamber area, causing the temperature of the air to equilibrate or become more consistent.
  • the fan 208 comprises an electrical fan.
  • the fan 208 is battery operated.
  • the fan 208 is enclosed, shielded, or substantially enclosed or shielded.
  • the fan 208 is in communication with one or more processors, controllers, microcontrollers, or circuits.
  • the fan 208 speed is capable of being controlled by the one or more processors, controllers, microcontrollers, or circuits.
  • the fan 208 speed is adjusted based on a temperature of a chamber area.
  • the fan 208 speed is adjusted based on a temperature of dewax process. a buffer liquid, as described herein.
  • the stainer system 200 includes a heater element 210.
  • the heater element is suitable for heating or increasing the temperature of the air HIER buffer in a chamber area.
  • the heater element 210 comprises an electrical heater.
  • the heater element 210 is battery operated.
  • the heater element 210 is enclosed, shielded, or substantially enclosed or shielded.
  • the heater element 210 is in communication with one or more processors, controllers, microcontrollers, or circuits.
  • the heater element 210 temperature is capable of being controlled by the one or more processors, controllers, microcontrollers, or circuits.
  • the heater element 210 temperature is adjusted based on a temperature of a 214 chamber area.
  • the heater element 210 temperature is adjusted based on a temperature of a buffer liquid, as described herein.
  • the stainer system 200 includes an air inlet/outlet 212.
  • the air inlet/outlet 212 forms an air-tight passage into a chamber area.
  • the air inlet/outlet 212 is capable of adding or removing air from a chamber area.
  • the air inlet/outlet 212 is connected to an air pump or similar device.
  • the air inlet/outlet 212 is in communication with one or more processors, controllers, microcontrollers, or circuits.
  • the air pressure in a chamber area is capable of being controlled by the one or more processors, controllers, microcontrollers, or circuits.
  • the air pressure is controlled by an adjustable air pressure control mechanism.
  • the air pressure in a chamber area is adjusted based on a temperature of a chamber area. In some embodiments, the air pressure in a chamber area is adjusted based on a temperature of a buffer area, as described herein.
  • the adjustable air pressure control mechanism comprises an air pressure measurement gauge or measurement device connected to a valve. In some embodiments, the valve is opened or closed to allow for air pressure adjustment.
  • the stainer system 200 includes a seating area 214.
  • the seating area 214 is capable of, or suitable for, holding a slide 214a or IHC utensil for staining. In some embodiments, stack a slide over another in an angle, so the chamber is capable to hold more slides per each staining process.
  • the slide or IHC utensil for staining includes a capillary gap cover that protects a tissue during an HEIR IHC staining process to ensure even heat distribution within a buffer pool.
  • the seating area 214 is capable of, or suitable for, containing a liquid, such as a buffer liquid.
  • the seating area 214 is capable of, or suitable for, containing a buffer pool 214b.
  • the seating area 214 includes or comprises a submersion system that submerges a slide within a buffer pool to eliminate formation of hot spots and micro nucleated boiling.
  • the seating area 214 comprises a material suitable for conducting heat.
  • the seating area 214 comprises a hard plastic metallic material prefer stainless steel.
  • the seating area 214 comprises a plexi-glass material.
  • the seating area 214 comprises a glass material.
  • the seating area 214 comprises a polypropylene material.
  • the seating area 214 comprises a polypropylene and polyethylene blend material.
  • the seating area 214 comprises an acrylic material. In some embodiments, the seating area 214 comprises another material. In some embodiments, the seating area 214 comprises a dark or colored material usable to block light. In some embodiments, a submersion system comprises a dish, bowl, or other device capable of containing a liquid.
  • the seating area 214 is removable attached to the container 204. In some embodiments, the seating area 214 is cup-shaped, rectangularly and cup-shaped, or U-shaped, and removably attached to the container 204. In some embodiments, the seating area 214 allows for suspension of an IHC slide, device, or utensil above a chamber area, allowing for heat conduction from the chamber area, through the seating area 214 or through a buffer liquid or pool.
  • the stainer system 200 includes a liquid drain 216 from the seating area 214.
  • the liquid drain 216 allows for removal of liquid, such as a liquid buffer, from the seating area 214.
  • the stainer system 200 includes a temperature sensor 218.
  • the temperature sensor 218 allows for determination of a temperature of a liquid such as a buffer liquid in which an IHC slide or device is immersed.
  • the temperature sensor 218 is in communication with one or more processors, controllers, microcontrollers, or circuits.
  • the temperature of a liquid, such as a buffer liquid is capable of being controlled by the one or more processors, controllers, microcontrollers, or circuits.
  • the temperature of a liquid, such as a buffer liquid is adjusted based on a temperature of a chamber area, or vice versa.
  • various systems, sub-systems, and components from the aforementioned are combined and in communication with the same controlling unit comprising one or more processors, controllers, microcontrollers, or circuits.
  • temperatures are continuously monitored by one or more temperature sensors. In some embodiments, temperatures are determined at predetermined intervals by one or more temperature sensors. For example, temperatures may be determined by one or more temperature sensors in a buffer liquid, chamber area, etc.
  • air pressures are continuously monitored by one or more pressure sensors. In some embodiments, air pressures are determined at predetermined intervals by one or more pressure sensors. For example, air pressures may be determined by one or more pressure sensors in a chamber area, etc.
  • the temperature and pressure readings and measurements from various sensors are delivered to a controlling unit, for example.
  • the controlling unit allows for synchronization, adjustment, and calibration of various system components based on various system environment measurements.
  • one or more signals may be delivered by the controlling unit to a heating element to increase or decrease the temperature so as to adjust the temperature of the buffer pool.
  • one or more signals may be delivered by the controlling unit to a compressor element or system to increase or decrease the air pressure by adding or removing air through an air inlet/outlet so as to adjust the air pressure of the chamber area.
  • physical chemistry relationships between temperature and pressure are used to adjust environment conditions in a chamber area or buffer pool. For example, pressure adjustments are used to modify boiling points and chamber temperatures and humidities for example, and vice versa.
  • the systems and methods herein are usable to dynamically control environment conditions, such as the conditions of a heating chamber or buffer pool, during a staining process.
  • environment conditions such as the conditions of a heating chamber or buffer pool
  • the systems and methods herein help to prevent tissue damage and bubble formation during staining.
  • FIG. 3 is a block diagram of system architecture 300 in accordance with some embodiments.
  • System architecture 300 includes a hardware processor 302 and a non-transitory, computer readable storage medium 304 encoded with, i.e., storing, the computer program code 306, i.e., a set of executable instructions.
  • Computer readable storage medium 304 is also encoded with instructions 307 for interfacing with external devices.
  • the processor 302 is electrically coupled to the computer readable storage medium 304 via a bus 308.
  • the processor 302 is also electrically coupled to an I/O interface 310 by bus 308.
  • a network interface 312 is also electrically connected to the processor 302 via bus 308.
  • Network interface 312 is connected to a network 314, so that processor 302 and computer readable storage medium 304 are capable of connecting to external elements via network 314.
  • the processor 302 is configured to execute the computer program code 306 encoded in the computer readable storage medium 304 in order to cause system architecture 300 to be usable for performing a portion or all of the operations as described herein.
  • the processor 302 is a central processing unit (CPU) , a multi-processor, a distributed processing system, an application specific integrated circuit (ASIC) , or a suitable processing unit.
  • CPU central processing unit
  • ASIC application specific integrated circuit
  • the computer readable storage medium 304 is an electronic, magnetic, optical, electromagnetic, infrared, or a semiconductor system (or apparatus or device) .
  • the computer readable storage medium 604 includes a semiconductor or solid-state memory, a magnetic tape, a removable computer diskette, a random access memory (RAM) , a read-only memory (ROM) , a rigid magnetic disk, or an optical disk.
  • the computer readable storage medium 304 includes a compact disk-read only memory (CD-ROM) , a compact disk-read/write (CD-R/W) , or a digital video disc (DVD) .
  • the storage medium 304 stores the computer program code 606 configured to cause system architecture 300 to perform a portion or all of the operations as described herein. In some embodiments, the storage medium 304 also stores information needed for performing a portion or all of the operations as described herein as well as information generated during performing a portion or all of the operations as described herein, such as a user interface parameter 316.
  • the storage medium 304 stores instructions 607 for interfacing with external devices.
  • the instructions 307 enable processor 302 to generate instructions readable by the external devices to effectively implement a portion or all of the operations as described herein.
  • System architecture 300 includes I/O interface 310.
  • I/O interface 310 is coupled to external circuitry.
  • I/O interface 310 includes a keyboard, keypad, mouse, trackball, trackpad, or cursor direction keys for communicating information and commands to processor 302.
  • System architecture 300 also includes network interface 312 coupled to the processor 302.
  • Network interface 312 allows computer architecture 600 to communicate with network 314, to which one or more other computer systems are connected.
  • Network interface 312 includes wireless network interfaces such as BLUETOOTH, WIFI, WIMAX, GPRS, or WCDMA; or wired network interface such as ETHERNET, USB, or IEEE-1394.
  • wireless network interfaces such as BLUETOOTH, WIFI, WIMAX, GPRS, or WCDMA
  • wired network interface such as ETHERNET, USB, or IEEE-1394.
  • the apparatus is another device capable of processing logical functions in order to perform the operations herein.
  • the controller and the storage unit need not be entirely separate devices, but share circuitry or one or more computer-readable mediums in some embodiments.
  • the storage unit includes a hard drive storing both the computer-executable instructions and the data accessed by the controller, and the controller includes a combination of a central processing unit (CPU) and RAM, in which the computer-executable instructions are able to be copied in whole or in part for execution by the CPU during performance of the operations herein.
  • CPU central processing unit
  • a program that is installed in the computer is capable of causing the computer to function as or perform operations associated with apparatuses of the embodiments described herein.
  • a program is executable by a processor to cause the computer to perform certain operations associated with some or all of the blocks of flowcharts and block diagrams described herein.
  • At least some embodiments are described with reference to flowcharts and block diagrams whose blocks represent (1) steps of processes in which operations are performed or (2) sections of a controller responsible for performing operations.
  • certain steps and sections are implemented by dedicated circuitry, programmable circuitry supplied with computer-readable instructions stored on computer-readable media, or processors supplied with computer-readable instructions stored on computer-readable media.
  • dedicated circuitry includes digital or analog hardware circuits and include integrated circuits (IC) or discrete circuits.
  • programmable circuitry includes reconfigurable hardware circuits including logical AND, OR, XOR, NAND, NOR, and other logical operations, flip-flops, registers, memory elements, etc., such as field-programmable gate arrays (FPGA) , programmable logic arrays (PLA) , etc.
  • FPGA field-programmable gate arrays
  • PLA programmable logic arrays
  • the computer readable storage medium includes a tangible device that is able to retain and store instructions for use by an instruction execution device.
  • the computer readable storage medium includes, for example, but is not limited to, an electronic storage device, a magnetic storage device, an optical storage device, an electromagnetic storage device, a semiconductor storage device, or any suitable combination of the foregoing.
  • a non-exhaustive list of more specific examples of the computer readable storage medium includes the following: a portable computer diskette, a hard disk, a random access memory (RAM) , a read-only memory (ROM) , an erasable programmable read-only memory (EPROM or Flash memory) , a static random access memory (SRAM) , a portable compact disc read-only memory (CD-ROM) , a digital versatile disk (DVD) , a memory stick, a floppy disk, a mechanically encoded device such as punch-cards or raised structures in a groove having instructions recorded thereon, and any suitable combination of the foregoing.
  • RAM random access memory
  • ROM read-only memory
  • EPROM or Flash memory erasable programmable read-only memory
  • SRAM static random access memory
  • CD-ROM compact disc read-only memory
  • DVD digital versatile disk
  • memory stick a floppy disk
  • a mechanically encoded device such as punch-cards or raised structures in a groove having instructions
  • a computer readable storage medium is not to be construed as being transitory signals per se, such as radio waves or other freely propagating electromagnetic waves, electromagnetic waves propagating through a waveguide or other transmission media (e.g., light pulses passing through a fiber-optic cable) , or electrical signals transmitted through a wire.
  • computer readable program instructions described herein are downloadable to respective computing/processing devices from a computer readable storage medium or to an external computer or external storage device via a network, for example, the Internet, a local area network, a wide area network or a wireless network.
  • the network includes copper transmission cables, optical transmission fibers, wireless transmission, routers, firewalls, switches, gateway computers or edge servers.
  • a network adapter card or network interface in each computing/processing device receives computer readable program instructions from the network and forwards the computer readable program instructions for storage in a computer readable storage medium within the respective computing/processing device.
  • computer readable program instructions for carrying out operations described above are assembler instructions, instruction-set-architecture (ISA) instructions, machine instructions, machine dependent instructions, microcode, firmware instructions, state-setting data, or either source code or object code written in any combination of one or more programming languages, including an object oriented programming language such as Smalltalk, C++ or the like, and conventional procedural programming languages, such as the C programming language or similar programming languages.
  • the computer readable program instructions are executed entirely on the user's computer, partly on the user's computer, as a stand-alone software package, partly on the user's computer and partly on a remote computer or entirely on the remote computer or server.
  • the remote computer is connected to the user's computer through any type of network, including a local area network (LAN) or a wide area network (WAN) , or the connection is made to an external computer (for example, through the Internet using an Internet Service Provider) .
  • electronic circuitry including, for example, programmable logic circuitry, field-programmable gate arrays (FPGA) , or programmable logic arrays (PLA) execute the computer readable program instructions by utilizing state information of the computer readable program instructions to individualize the electronic circuitry, in order to perform aspects of the subject disclosure.
  • a Legend 6G Stainer includes a dynamically controlled external air pressurized heating chamber designed for a Heat Induced Epitope Retrieval (HIER) immunohistochemistry (IHC) staining process, said heating chamber being of low mass and capable of being pressurized by external air.
  • the Legend 6G Stainer further includes an adjustable air pressure control mechanism that regulates the pressurization of the heating chamber.
  • the Legend 6G Stainer further includes a capillary gap cover that protects a tissue during the HIER IHC staining process to ensure even heat distribution within a buffer pool.
  • the Legend 6G Stainer further includes a submersion system that submerges a slide within the buffer pool to eliminate formation of hot spots and micro nucleated boiling, the heating chamber and associated components allowing users to adjust a pressure within a range of 8 to 12 psi, thereby providing control over a boiling point of the HIER IHC staining process for different tissue types and staining protocol requirements.
  • the Legend 6G Stainer of Supplemental Note 1 wherein the heating chamber maintains a temperature range and a boing point between 100 and 130°C, reducing the risk of tissue damage and enabling precise control during the HIER IHC staining process.
  • the Legend 6G Stainer of any of Supplemental Notes 1-2 further comprising a pressure control system that adjusts the air pressure in real-time to prevent the formation of bubbles, ensuring effective antigen retrieval and staining.
  • a staining system includes a dynamically controlled external air pressurized heating chamber designed for a Heat Induced Epitope Retrieval (HIER) immunohistochemistry (IHC) staining process, said heating chamber being of low mass and capable of being pressurized by external air.
  • the staining system further includes an adjustable air pressure control mechanism configured to regulate the pressurization of the heating chamber.
  • the staining system further includes a capillary gap cover that protects a tissue during the HIER IHC staining process to ensure even heat distribution within a buffer pool.
  • the staining system further includes a submersion system that submerges a slide within the buffer pool to eliminate formation of hot spots and micro nucleated boiling, the heating chamber and associated components allowing users to adjust a pressure within a range of 8 to 12 psi, thereby providing control over a boiling point of the HIER IHC staining process for different tissue types and staining protocol requirements.
  • the staining system of Supplemental Note 6 wherein the heating chamber maintains a temperature range and a boing point between 100 and 130°C, reducing the risk of tissue damage and enabling precise control during the HIER IHC staining process.
  • the staining system of any of Supplemental Notes 6-7 further comprising a pressure control system configured to adjust the air pressure in real-time to prevent the formation of bubbles, ensuring effective antigen retrieval and staining.
  • the staining system of any of Supplemental Notes 6-9 wherein the capillary gap cover protects the slide from uneven heating and other irregularities that lead to formation of bubbles, preventing staining issues, and breaking off of cell clusters.
  • a staining method comprising dynamically controlling an external air pressurized heating chamber designed for a Heat Induced Epitope Retrieval (HIER) immunohistochemistry (IHC) staining process, said heating chamber being of low mass and capable of being pressurized by external air, regulating the pressurization of the heating chamber using an adjustable air pressure control mechanism, ensuring even heat distribution within a buffer pool using a capillary gap cover that protects a tissue during the HIER IHC staining process, submerging a slide within the buffer pool to eliminate formation of hot spots and micro nucleated boiling using a submersion system, allowing users to adjust a pressure within a range of 8 to 12 psi, thereby providing control over a boiling point of the HIER IHC staining process for different tissue types and staining protocol requirements using the heating chamber and associated components.
  • HIER Heat Induced Epitope Retrieval
  • IHC immunohistochemistry
  • the staining system of Supplemental Note 11 further comprising maintaining a temperature range and a boing point between 100 and 130°C, reducing the risk of tissue damage and enabling precise control during the HIER IHC staining process using the heating chamber.
  • the staining method of any of Supplemental Notes 11-12 further comprising adjusting the air pressure in real-time to prevent the formation of bubbles, ensuring effective antigen retrieval and staining using a pressure control system.
  • the staining method of any of Supplemental Notes 11-14 further comprising protecting the slide from uneven heating and other irregularities that lead to formation of bubbles, preventing staining issues, and breaking off of cell clusters using the capillary gap cover.

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Abstract

L'invention concerne un système de coloration (200) comprenant une chambre de chauffage sous pression d'air externe à commande dynamique pour un processus de coloration d'immunohistochimie (IHC) de récupération d'épitopes induite par la chaleur (HIER), ladite chambre de chauffage étant de faible masse et pouvant être mise sous pression par de l'air externe. Le système de coloration (200) comprend un mécanisme de commande de pression d'air réglable qui régule la mise sous pression de la chambre de chauffage. Le système de coloration (200) comprend en outre un couvercle à espace capillaire qui protège un tissu pendant le processus de coloration IHC HIER pour assurer une distribution de chaleur uniforme à l'intérieur d'un groupe de tampons. Le système de coloration (200) comprend en outre un système d'immersion qui immerge une lame à l'intérieur du groupe de tampons pour éliminer la formation de points chauds et l'ébullition micro-nucléée, la chambre de chauffage et les composants associés permettant aux utilisateurs d'ajuster une pression dans une plage de 8 à 12 psi, ce qui permet de fournir une commande sur un point d'ébullition du processus de coloration IHC HIER pour des pH et des exigences de protocole de coloration de différents types de tissu.
PCT/CN2023/122724 2023-09-28 2023-09-28 Systèmes et procédés de coloration d'immunohistochimie (ihc) à commande dynamique Pending WO2025065564A1 (fr)

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Citations (6)

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WO2016064760A1 (fr) * 2014-10-19 2016-04-28 Peddinti Kamal Prasad Dispositif de coloration par lot automatisé pour l'immunohistochimie
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WO2016064760A1 (fr) * 2014-10-19 2016-04-28 Peddinti Kamal Prasad Dispositif de coloration par lot automatisé pour l'immunohistochimie
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