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WO2025064843A1 - Stabilisation d'enzymes sur une brosse de polymères mixtes au moyen de groupes aromatiques - Google Patents

Stabilisation d'enzymes sur une brosse de polymères mixtes au moyen de groupes aromatiques Download PDF

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Publication number
WO2025064843A1
WO2025064843A1 PCT/US2024/047728 US2024047728W WO2025064843A1 WO 2025064843 A1 WO2025064843 A1 WO 2025064843A1 US 2024047728 W US2024047728 W US 2024047728W WO 2025064843 A1 WO2025064843 A1 WO 2025064843A1
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eer
meacrae
acrame
meacrame
acrae
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Joel L. Kaar
Daniel K. Schwartz
Hector Sanchez-Moran
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University of Colorado System
University of Colorado Colorado Springs
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University of Colorado Colorado Springs
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
    • C08J7/12—Chemical modification
    • C08J7/16—Chemical modification with polymerisable compounds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/10—Esters
    • C08F220/38—Esters containing sulfur
    • C08F220/387—Esters containing sulfur and containing nitrogen and oxygen
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F290/00—Macromolecular compounds obtained by polymerising monomers on to polymers modified by introduction of aliphatic unsaturated end or side groups
    • C08F290/02—Macromolecular compounds obtained by polymerising monomers on to polymers modified by introduction of aliphatic unsaturated end or side groups on to polymers modified by introduction of unsaturated end groups
    • C08F290/06—Polymers provided for in subclass C08G
    • C08F290/062—Polyethers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F2438/00—Living radical polymerisation
    • C08F2438/01—Atom Transfer Radical Polymerization [ATRP] or reverse ATRP
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2333/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
    • C08J2333/04—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters
    • C08J2333/06—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters of esters containing only carbon, hydrogen, and oxygen, the oxygen atom being present only as part of the carboxyl radical
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2333/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers
    • C08J2333/04—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters
    • C08J2333/14—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides, or nitriles thereof; Derivatives of such polymers esters of esters containing halogen, nitrogen, sulfur, or oxygen atoms in addition to the carboxy oxygen

Definitions

  • monomern eresennvenonroves a co-omerrusavn an aromac r comnaon w a non-aromac monomer.
  • co-omerrus accornoeos sa asezce c aann enazvem aen ennzeme aeraeco.eoe coe- cooomemrerrursus a.ccore cno-oomeerrsrus assecou cnnocouem an aona monomer or mo an c eaacaes aacmen o an enzmeoe cocroner m ceerruasn.
  • eaecrrso asamec,e non-aromac monomers seecerome meacrooxe rmeammonumn coorormeammonum core,- suoro acrae,-suoro acramre,-suoro meacrae,- meacrooxe me--suoroamme,on-suoro meacrame,- ac, acrame, meacrame,o umroxe, meacrc ac, acrc acrae,oeeneco acraemee,neco meacrae,oeeneco eamnoe meacrae,-eamonoeeene acco meacrame,- acrame, -eamnoe meacrame, nr-saoer,oa-eamnoe moronoe meacrae,--moronoe acrae,-c
  • c ac monomerneomerrus accornoer asec cane eene eoeneenc eer meacrae ,en meacrae,enz meacrae,eraeenoen eer meacrae,reenecoen eer meacrae, oeecoen eer meacrae,enaeenecoen eer meacrae, meacraenee,-cro en eer meacrae,-a meacrae,-a een eneme meacrae an-nraceneme meacrae, srene, eer aceraceo,reenen eeer accoraeen, eener a accrraaee,,eernaze acerneae,coeenene eecroen enaeenecoen eer acrae,oeenecoen eer ac
  • c c nes reresen caracersceasor aromac- scaern.or eac comoness oeraeomeraer measure namc raus oeomer- son,e raus oe core 2 arce was suraceromeecnca recaesn rae, acacorucne.nrroorrraorsa reareosnen o eerr soran reaare erroor oe se meanorree core scaarce.
  • curnov ervaeweeneowes an esurnover o anea.nzmac acve rer oeno ao oaneromaaonsneaseox o rrorars reresene sanar enroe ommoze urnncuaon ao. measuremenn .
  • e rror oe mean oreeecnca recaesor eac o curvesrom..rroeorrearcsa esomaaee va ororoaucaoonrm oanone croarrees eorrnorn areoncnueeraon areoo smao u anure a.romsa ae s o ces re oerve surea.
  • a mue seuence anmen o w-e ma WT aesne muans.oven-exose aromac resues are en eacenoas, aonn anure mu aaronm aarec s reoswnuees aorwee w-een s ceaune.nce.e reacemen amno acsor ao.suo aar oras sown oca asoron secra oaexauor an c roores.n eaco,e correconacorsor overan snas s uocuroaeore.n n eoarcmazon,ewo an waveen nms s an wascoe’s a asre ae maexm arae m aasorance o eac .s ara sowne overa reoneween
  • annseausr oa aovno sonwn aro surace-exose aromac resues onacus sus urer sazaon o v macneracons,ese cane exoeoromoeemeraureeenenc.romaac-s-ascacnn.sos monreeremmoeercauuarrennseernacsovnsaveronounce .s anusraon sown-sac e. .s a scem n w aomeracone.
  • nerranser .e acs sownnramoecuarrseresonance azoenaa -rece muaeness aoweenroucon o csene an- seccaenn wnez a z an ses.c oroona cemsres aowe se- orser raus: . nm-.areuoroores s –exa anaeme –o .
  • ee omaes an scemacs sown - eermnenamc unoeracon vram aauae enaecurn conons , suoreo er unon.ucuaon : caerone-easer re-on an sownown oncrease anass enaes measurn r oeoe an unoe saes.n ase mnos re anoc “sens”eoe sae va reucnucuaons.
  • eacaosrs an re asnoonrsmaeoarr enez ammoeu an ore suaraocne.-exeose aromac resues wc coue amonncreasene omaem mara,ncororaon o aromacseo an ne-moecue eraure o o, as we as a-o enancemenn acv. romon enzme reo sune asn re sveaaeznaee esnez sume’osrso ace a asno cmaamecca ca acervoene ssae.
  • cemcaeeroeneous suor maerassrre saznneraconsnonamc anmmoze enzmes e suc mecansmosv ces comex sazaon mecansmsor convenona caracerzaon meos, sne moeane caennosenane usn mcroscoasrove unue mecancsuerser resonance enerranser- suors w aroeruneaance o enzme-omcernsns,emonsrananamc ommoze enzmes sazneoe sae anoerracaonsmroveeerormance “caerone-e” eec.araonsnomer srucure ma can reon.e., va ammoze oer new ooruneso enance mmo enzzameoner oormaancce.
  • oan mecanscnssnoe comex unon an reons onammmcoze anaer.vera,e comne macroscoc an moecuar-sca s wnerusmorance oroeruneneracons e oservaons unerscoree suor an enzme surace re eween aromac moeesnemmozaonmmoze enzme suesorreservn an rescuneoe an acve sae o saus.
  • uez,on..,ovaenene,m.m.o,czawaornz,e.as.oaaar,.-.cevuceranez-ome.ounranmcs uon mercanemcaoce , - o a oe r na, oerasesa are more aooc areeer saze essroc mxeomerruses conann sonveno-eexoseene aromcaoc m reesaucres,a acec.onunerensnor ⁇ , oas an unusua comoson o on areessroocanac s surace.e aromacacesoun roocases esorme aac resues, wc areresenn more nersan .ncez-orn,.,ez,..,cwarz,..
  • aar aar,.. an nesnuesormzneneraceeweenmmozenzmes an uroemrmoroe,oemerruses. eaerasneraces, - care a suraceroc o s asoueoeresence o numerous oservaonns, warsooc reesszeuesasne, m aarneneu,r asera sraae,zeuamanero.ucase onese w aromacrous wn ruses, wc ma seccan n monomers sacn an caonc-caon resues.ese a erac w aromac- oerneracons w ,er romacrous ma asooenaromoe moeesa surrou oocneracons we soven exose aac erorma n ’s acve
  • oer wors s o *moereso_a moes o monomere..., .n ncororaon o was conrme usnmoes_ moes_ moes_ . secrosco, wc reveae a ssemacncrease ouse ree areocmaanceco-ur serr-ercansnorm monerarencreasn conen ..aracerzaon oeomer-moearc w namc scaern sueseae sovaeomer corona was- es c.as, we veree wea o arusesrown ona sca wa nmc .
  • amouns oo seusrsac wea-esxeossee aneusrenn aromraeec reserueens were m vuaraaenson a warmceexeo rseave meonnevaneure.
  • muans wereesnae , w soven-accees or aromac surace resues muaeo reucee aromac s DSA-50% se a surace aromac resues remove urace area , DSA-100% , w removeae,..e , an Dburied-100% , w aure aromac resues a sraomac resues accessensee muoanes saaceae D e S s A-5 n 0% o an wee D r S r A e-m 10 o 0 vn res suoveenn-e rxeuocsee
  • ane omea muans were o sumaor saorze on suor.s w.esso ormmoze muansa reuceerma sa rea WT veommozena, a,osree acv aoveo, evenou some oe muans were more acve an sa WT n ..ese oservaons suesea aromac resuesn ae anemaonr WT nromon saznneracons w -conann suors W , T suornean roe aen-asa mcecnan ansms m-caa aonso areenev oess o ronveen,nncuen saezoarmona oon ommorozeoc nc W e T s.
  • nmneoe sae o reaveo aarnermar se 0 u resunn .eonor an acceor were asoar was ence, resunnare substituted with unoe sae.s substituted with unoe sae o va a se-avon wa us,,en an averaener-uorooresance o aroxmae. nm ..n su aecar ene creecerease-n o w case emxceaceen uauornaon, w-c re usnuoenn a.
  • ea sms unererma sae anermaenaurn were coeceo enae sasccaem reroarouures, m aeaasruere nmuemne or ooraemceo-raevserae-uaneasee.., avoenraor-eacoceeorracsoonra amneanmamasc waeraremeeenrserae.e., araren unon an reon raes. comoson an were useoenoe om a oservaonsor eacrus n eac exermena reazaon.
  • cemcas were use w e wasurcaseromees wo-ncuse sca waersor sneou mouererurcaon.
  • csanana,.wo-nc sc cueman wereurcaseromar Inse on waers w nave oxe coanor esomer were
  • senvrro rom omenae eer rxmaro ouoa sraee, erac-. am s,rane.enz- ccoocone-ma-se was Inserom orema me was uracerearaon, .ous,.
  • vvor mnues.ereoson was comee, waers were nouenex,soroanox an waer, an soren a v e rnse w urace-naeomerza acuumesccaor unurer use.
  • ecvaorseenerae econs o mxe ruses were snesze meo.ez,.. ronranseromranseracaomerzaon aa ,ene,..,cwarz,..aar,..ramacncreasen acaersosrm,ance o-mmozeonaseosewcz,er.
  • -nsrumenr wces were caracerze va usn aermosercenccoe aareszer were ae sameoer.namc scaern measuremensnon uer a . anroer soonrme aer resusensonn.mere m soumosae or sne moecue mccarosocnoor, sarcocne oxseers woane.
  • vvroeneroxe suurc acor, rnrsse were ceanenrana souon nroen, an exoseo-ozoneor.er e wos uonr,aure waer,re w erorme smar asescreorarces,un a camer omerzaons were ea o suraces was measure conac aneono w anner 2 amosere. w a cusom mer vae sessero meo m-uuansonesomne aenr.
  • e consruc oacus suasenas moe verson o arevousescre a e was exresse recomnan w a oner,.,azen,..,acson, uonuconrooco.amm,..,ae- uornaeroensor- .
  • m.ncuaon was carre ouor a exresson, ces werearves ssroecerom n aumnumo.oown suseuenasrozen uneur vcaa coenruaon a rm aournmn an ao,raourr mesc.a aroen va ,, c a mesn wmer cer az roem osua eson anenra. en wa uanus caenrrre e, c oou ronca wnon a-m so couummnoso-aae, esae a re, anse va soncaon.
  • exncon coecen o, -1 cm -1 .se va- an secrooomer usn an ..,eanee,n. w.,as ccarwraerz o,u. w. a moaar,e. v.ersraomna ocrenvcroeuassenescre meos.ez, mmozeases erazaon onomerrusuoaacerormance o .an,.,amar,.. uer,.
  • m o enaemmozaon,uressa ensz:mes wereuer excanen nzmomeza.o mnu waers cnocnussaen w o o an e meocmneer,rus-.co,aem nanosaer.es sou mon o us enac revovn ora saer.
  • enzmes were cacuaeor ao.ermmoz a enzmeoan onearces waseermne usn a massaanc aon, o eac enzme remannne suernaan ease one reave acv normazne acv oe enz oe reacon.eave acv was cacuae asence oarces.o ensurea amne nnon-ceov saueenrna aansoroee e anczvme w oas reem eonvzemeromnee mom soerumrusaer,earces were wase muemes wresuer conssn o emoseraaue a une enzmac acv oe suernaan was ne e.
  • uoroax- s re-eenen acvroes were measure usn aoracenc aorncecrouoromeer.nec sa roes were carre ou usn arean concenraouna oon,r aene a anecmanmonnzeero enzmeaen re eaacer aosrsauo rreeascceonnce, measuremens.e speechous, was normazease one enzmeoan one m wc was mxe carre oun m soumosaeuer a , usncros reeres.eacons were uoroenc susrae.
  • mm rausorovcseen scca suorrou ause sca macn o.e emsson oo onor ano us anc ause sca reracvenex- were mineeno secrasncman cannes uceoruorooresrom - ,roma.eonor sna wasurer ceane usnsn a a n nmcroc mrror emroc, ane acceor sna was ceane w a nmanassmaenreasser onor an acceorman cannes were ane usn anos eam semroc.
  • eamas wereenerae u r or acceoruorooresransonarameers we sn aaaonsrom eacraecor,nec escre.ez,..,ree ensem,a.e., uscnw aarz,e.n.aroavar,.an. wramarecen scareeass aesrevous erormance ommoze ases erazaon onomerrusunaac araress, - ene,..,aaa ors. o c,..,aar,..cwarz,..
  • w,eresseee oave-nraneraeweenonor s emsson an acceor’s excaon secra exncon c D oecen o aocrcmeazeuorescence emsson secrum oeonor + ,
  • a se moar orenaonacoreweenonoorr, ans w acacveeeonr, w, 0 serser raus, Consequentlyse reave resecveners are sucenon anexeocesareeen ausoro,o arsesu romanon naer reracvenex oeranser meum an se D 2 oeesnrueo unn ooneer s-aueor ooore vsaancoenseoaro smuaon: va aonearo smua re unoe sae o was moee m on usn a
  • o ae mse uasne aroeuas ooue enre acaon,eerms “a” an “an” are usene sense comonens or ses, unesnse”,e “a coneaesx a cearsr”, “ocnaee osr o moerrew”s oer. “aor euxraam”e, oe reerencencues aura o ces, eerm “a ce” er ceerm “anoncun mxuresereo.
  • an morereera wnxm oa ae”v as useeren means wn,reera wn numerca rearneasn, amnouen os,e vraauens a enxamerceese n,n oar va e unuees osr o ranerew.se exress sece, a oe anemeraures o reacon s suc asoseor amouns o maeras,mes averae mo , raos o amouns, vauesor moecuar we weer numer oownoecuar we“ n ” or we averae moecuar we“ w ”, an oersne eerm “aron oe seccaon mae rea asreace e wor “aou” evenouncaeooue” c monara nro, exer neusss
  • e vereas an no as an aemosm soue a souca aonea osee cooncsrrunee on euva oense noume scoe oe cams, eac numercaarameer ornar r er o reore sncan s an an escooou suwnn re asreanec annu roxaes.
  • numerca vaue,owev ecc exames are necessar resunrome sanarev er,neren conan ceran errors urermore, wen numer aonounner resecveesn measuremens.
  • an comnaon oeca ranes o varn scoe are seoreren,s conemaea s useeren,see vaerumes “cnocmusve oe rece vaues mae use.
  • an meosncuee reerencrsn”sneneo meanaeroucs, comosons essena o” wen use e comonens or ses,u no excun oers.
  • onssn oer comonens o oeneroucs comosons an meos, sa mean excun oe rece comorn seenss o wo aun e nsose enxcau senraccea cnocne.amunsa,n a cso amnosaromna ccoenuscsan e ascsceena carrers.
  • “onssn o” sa mean excun moreanrace eemens o oe ae ses.
  • sasca coomers aomeseo aomoomer were are arranen a seuenceaoows some s rn wcwo or more monomers euaoeroa onn a resue oaas mcoa rue.e moeracon o a monomeres a ranomomer.eseomers areener nomer a anonne can,eomer meo.
  • vaeree racaomerzaon suraces w uesere aeorenm,erererm “ase maera” reerso a susraerovn one or more eraeo or oer us canerownrome surace, oro wcomerruses can we vare ouncwosnea axe.s conemaeaeomerruses cane axeo a oroene.
  • ze suraces,ncun sca, resns,omereas, aarose, an sormesr uosme aeorenm,erezaeromn s “urussra”e ora “von am rearcraus-”o remeersrzoa aeoermmenrac sroue cana eomerzae susraesease maera, or cane enraeo or oe , did ase maera,ere su rwse axeoe s us sanaaneorm oease maera.
  • caarcanen ae rarencaeneurcme “reraacnve r meaocnoomne.r” ree reerascove a m coomnomouern canaes an ca maaeer oa cae o mormnomers asescre aove aneren,or exameu nomeo e same ceacrae , or eene.ease maera an reacve monomer mae o oromeromoun,or exame, aoeenease maera ma uze eeene monomers an manuss acon urrereacra e.c.,nn re aea ac mceon aeo.
  • osr o a ceonnavenneron co amrerursnerrove.
  • n o sa mean excun moreanrace eemens o oer comonens or ar e avanaes seor aove, anose mae aarenromeoreonescron, eea erncenrom aea snceo.e oncee cenrvaenno cn,anessn meanee maa ae mnaeer as coovnea cnoensrnuceono wreoonu aesmcrnon se or sownne accomannrawns saenerree asusrave an non rnesee.
  • reerenceorenece esx cene nonnceoresen acaon arencororaener enreeren oreon wescre soeenae avnanssaeens seereowr. aove, anose mae aarenrome consrucon woun, aerea erncenrom aaen se an snce ceran canes mae maene aove conaneneoreonescron or socwonen oe aeccnovmenanon,nrsanenea a maers asusrave an non amn sen wns saenerree an secsc aseoauorees u oneresonovenase one.
  • o o waereee.raumeceorr oes r aenmaaznenr aaeecro aresnor eacer snn cree mroaeceuscer exeernmeen ceonoson se.coens.e . c meac . x -asco rae . erc ac ur 2 u wre - w caaaes..ereo rnearre rasenoss oe reaerecnen rsaanoes.
  • oe raos are r neoacrmoanszeo oro eeac se cnoonceesmnser oraorouns omearec crou.seesr
  • exareesse wenes a o-ermn maueaxnas- wscne weare cnarraocuecrescn ooe exr-esso.n veecsoer.enes were -onomeenrecoen eer meacrae rucure --nraceneme meacrae ae.rucures o seec aromac monomersor coomerruses. onomer rucure ae . .

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Abstract

L'invention concerne des brosses de copolymères aléatoires comportant un monomère aromatique méthacrylate d'éthylène glycol phényl éther (EGPMA) et un méthacrylate de sulfobétaïne (SBMA) zwitterionique pour stabiliser des enzymes qui sont immobilisées sur la couche de brosse. Il est démontré ici que l'incorporation de 5 % d'EGPMA produit la stabilisation spectaculaire de la lipase immobilisée, ce qui a conduit à une augmentation de 50 °C de la température optimale de l'enzyme. Il a été observé que l'enzyme stabilisée à des températures élevées présente une activité catalytique 50 fois supérieure par rapport à l'enzyme non modifiée. Il est à noter que, en comparaison avec des surfaces de brosses antérieures, l'incorporation d'EGPMA permet de nouveaux types d'interactions avec l'enzyme qui contribuent à cet effet stabilisant.
PCT/US2024/047728 2023-09-20 2024-09-20 Stabilisation d'enzymes sur une brosse de polymères mixtes au moyen de groupes aromatiques Pending WO2025064843A1 (fr)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140045398A1 (en) * 2008-12-05 2014-02-13 Semprus Biosciences Corporation Layered non-fouling, antimicrobial antithrombogenic coatings
US20150045515A1 (en) * 2010-06-09 2015-02-12 Jun Li Non-fouling, anti-microbial, anti-thrombogenic graft-from compositions
WO2022104385A2 (fr) * 2020-11-13 2022-05-19 The Regents Of The University Of Colorado, A Body Corporate Stabilité et activité d'enzymes par immobilisation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140045398A1 (en) * 2008-12-05 2014-02-13 Semprus Biosciences Corporation Layered non-fouling, antimicrobial antithrombogenic coatings
US20150045515A1 (en) * 2010-06-09 2015-02-12 Jun Li Non-fouling, anti-microbial, anti-thrombogenic graft-from compositions
WO2022104385A2 (fr) * 2020-11-13 2022-05-19 The Regents Of The University Of Colorado, A Body Corporate Stabilité et activité d'enzymes par immobilisation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SÁNCHEZ-MORÁN HÉCTOR, KAAR JOEL L., SCHWARTZ DANIEL K.: "Supra-biological performance of immobilized enzymes enabled by chaperone-like specific non-covalent interactions", NATURE COMMUNICATIONS, NATURE PUBLISHING GROUP, UK, vol. 15, no. 1, 1 March 2024 (2024-03-01), UK, pages 2299 - 2299, XP093295809, ISSN: 2041-1723, DOI: 10.1038/s41467-024-46719-5 *

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