[go: up one dir, main page]

WO2025064425A1 - Système d'analyse d'échantillons biologiques - Google Patents

Système d'analyse d'échantillons biologiques Download PDF

Info

Publication number
WO2025064425A1
WO2025064425A1 PCT/US2024/047097 US2024047097W WO2025064425A1 WO 2025064425 A1 WO2025064425 A1 WO 2025064425A1 US 2024047097 W US2024047097 W US 2024047097W WO 2025064425 A1 WO2025064425 A1 WO 2025064425A1
Authority
WO
WIPO (PCT)
Prior art keywords
bio
sample
spectral optical
light
optical sensor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/047097
Other languages
English (en)
Inventor
Mohiudeen AZHAR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics Inc
Original Assignee
Siemens Healthcare Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Siemens Healthcare Diagnostics Inc filed Critical Siemens Healthcare Diagnostics Inc
Publication of WO2025064425A1 publication Critical patent/WO2025064425A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes

Definitions

  • the present embodiments generally relate to medical equipment. More specifically, the present embodiments are related to a system for performing bio-chemical tests for medical use.
  • CH chemistry
  • Figure 1 illustrates a conventional spectrophotometer 100 for analyzing bio-samples, in accordance with prior art.
  • the conventional spectrophotometer 100 includes a reaction ring 102 having a plurality of holders (not illustrated). Each holder of the plurality of holders accommodates a specimen container carrying a bio-sample to be analyzed.
  • the conventional spectrophotometer 100 further includes a lamp 104 for emitting a light towards the specimen container carrying the bio-sample. The light incidents on the biosample and incidents on a photometer 106.
  • the photometer 106 includes multiple lens 108 for directing the light towards a photo detector assembly 110.
  • the photo detector assembly 110 includes dichroic splitters 112-1 to 112-n for reflecting the light having a specified wavelength and transmitting the light having a wavelength other than the specified wavelength.
  • the dichroic splitter 112-1 reflects the light having wavelength of 805 nm and transmits the light having waving other than the wavelength of 805 nm.
  • the dichroic splitter 112-n reflects the light having wavelength of 658nm and transmits the light having a wavelength other than the wavelength of 658 nm.
  • the photo detector assembly 110 further includes band pass filters 114-1 to 114-n for allowing the light having a selected frequency range and blocking the light having a frequency other than the selected frequency range.
  • the photo detector assembly 110 further includes photodiodes 1 16-1 to 116-n for detecting the light passed through the band pass filters 114-1 to 114-n.
  • the conventional spectrophotometer 100 utilizes multiple elements, such as dichroic splitters, photodiodes, and band pass filters. Usage of multiple elements leads to increase in overall size, cost of manufacturing, and complexity of the luminometer.
  • the lamp 104 of the conventional spectrophotometer 100 requires a liquid coolant for cooling purpose.
  • the liquid coolant is to be frequently refilled manually in the lamp 100.
  • the problem of frequent refilling of the liquid coolant in the conventional spectrophotometer 100 is not addressed yet.
  • the usage of the liquid coolant in the lamp 104 increases an operational cost of the conventional spectrophotometer 100.
  • the present embodiments may obviate one or more of the drawbacks or limitations in the related art.
  • a system for analyzing bio-samples is provided.
  • the properties of multiple bio-samples are analyzed simultaneously.
  • the complexity is reduced by hardware reduction in a system for analysis of bio-samples.
  • the power consumption of the system for analyzing of biosamples is reduced.
  • the system for analyzing bio-samples includes a reaction ring including a plurality of holders. Each holder of the plurality of holders is configured to accommodate a specimen container containing a bio-sample for analysis.
  • the system further includes at least one light source positioned at a location within the reaction ring to emit light towards the bio-sample.
  • At least one multi-spectral optical sensor is positioned outside the reaction ring to capture the light passed through the biosample, for determining one or more parameters of the bio-sample.
  • the at least one light source is a light emitting diode (LED) emitting white light.
  • LED light emitting diode
  • a plurality of multi-spectral digital sensors are positioned at multiple locations outside the reaction ring to capture the light passed through the bio-sample to increase the accuracy of detection of the light.
  • the multi-spectral optical sensor is operable in a whole spectrum range.
  • the bio-samples include blood, urine, liquid bone marrow, and amniotic fluids.
  • the one or more parameters of the bio-sample that may be determined include levels of plasma, metabolites, electrolytes, drugs, and proteins.
  • the multi-spectral optical sensor is developed using standard CMOS silicon via nano-optic deposited interference fdter technology.
  • each multi-spectral optical sensor of the plurality of multi-spectral optical sensors is positioned to capture the light passing through a bio-sample held in a respective specimen container.
  • Figure 1 illustrates a conventional spectrophotometer for analyzing bio-samples, in accordance with prior-art.
  • Figure 3 illustrates a block diagram of a system for analyzing multiple bio-samples simultaneously, in accordance with an embodiment.
  • the present embodiments pertain to a system for analyzing bio-samples by calculating a concentration of a certain substances in a bio-sample.
  • the bio-sample may be blood, urine, liquid bone marrow, and amniotic fluid.
  • FIG. 2 illustrates a block diagram of a system 200 for analyzing bio-samples, in accordance with an embodiment.
  • the system 200 may include a reaction ring 202 including a holder 204.
  • the holder 204 may accommodate a specimen container 206 containing a biosample for analysis.
  • the specimen container may be a test tube or a beaker made from a transparent material.
  • the specimen container may be of cylindrical or cuboidal shape.
  • the holder 204 may be configured to cause minimal interference with transmission of the light passing through the specimen container 206.
  • the holder 204 may be of a shape that may hold the specimen container 206 effectively.
  • the system 200 may further include a light source 208 for emitting light towards the bio-sample.
  • the light source 208 may be positioned at any location within the reaction ring 202, such as at center of the reaction ring 202.
  • the light source 208 may be a light emitting diode (LED) emitting white light.
  • the system 200 may further include a multi-spectral optical sensor 210 for detecting the light passing through the bio-sample.
  • the multi-spectral optical sensor 210 is operable in one or more of Infrared region, visible region, and ultraviolet region.
  • the multi-spectral optical sensor 210 may be developed using a semiconductor technology where filters are integrated into standard complementary metal-oxide-semiconductor (CMOS) silicon via nano-optic deposited interference filter technology. Comparative analysis of the white light and the light passing through the bio-sample indicates one or more components of the white light absorbed by the bio-sample.
  • the one or more components of the white light absorbed by the bio-sample are indicative of one or more parameters associated with the bio-sample.
  • the one or more parameters of the bio-sample may include levels of plasma, metabolites, electrolytes, drugs, and proteins.
  • multiple multi-spectral optical sensors 210 may be used for detecting the light passing through the bio-sample. Such arrangement increases accuracy of detection of the light passing through the bio-sample and is beneficial in circumstances where any multi-spectral optical sensor becomes faulty or calibration of any multi-spectral optical sensor gets affected.
  • multiple bio-samples may be simultaneously analyzed using a system 300, as illustrated in Figure 3.
  • the system 300 may include a reaction ring 302 including a plurality of holders 304. Each holder of the plurality of holders 304 accommodates a specimen container containing a bio-sample for analysis.
  • the system 300 may further include a plurality of LEDs 306 positioned within the reaction ring 302. The plurality of LEDs 306 simultaneously emit light towards the multiple bio-samples held in the plurality of holders 304.
  • the system 300 may further include a plurality of multi-spectral optical sensors 308 positioned outside the reaction ring 302.
  • the plurality of multi-spectral optical sensors 308 simultaneously detect the light passing through respective bio-sample positioned next to the plurality of multi-spectral optical sensors 308.
  • the light detected by the plurality of multi- spectral optical sensors 308 may be utilized to determine one or more parameters associated with each of the multiple bio-samples. Analysis of the multiple bio-samples at once increases throughput of the system 300.
  • a system for analyzing bio-samples utilizes an LED for illumination and a multi-spectral optical sensor for detecting the light emitted by the LED.
  • the LED does not need any coolant, as the LED has a low thermal profile.
  • the multi-spectral optical sensor has multiple filters integrated into standard CMOS silicon via nano-optic deposited interference filter technology.
  • the multi-spectral optical sensor is able to detect the light of multiple wavelengths without requiring bulky hardware, such as dichroic splitters, photodiodes, and band pass filters.
  • the compact and economical multi-spectral optical sensor results in simultaneous analysis of multiple bio-samples. As a result, readout time of the system is reduced and throughput of the system is increased.

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

L'invention concerne un système (200) d'analyse d'échantillons biologiques. Le système (200) comprend une couronne de réaction (202) comprenant une pluralité de supports (204). Chaque support (204) de la pluralité de supports (204) est conçu pour recevoir un récipient (206) à échantillon contenant un échantillon biologique devant être analysé. Au moins une source de lumière (208), en particulier une diode électroluminescente (DEL), est positionnée à l'intérieur de la couronne de réaction (202) pour émettre de la lumière en direction de l'échantillon biologique. Le système (200) comprend en outre au moins un capteur optique multispectral (210) positionné à l'extérieur de la couronne de réaction (202) pour capturer la lumière ayant traversé l'échantillon biologique, pour déterminer une ou plusieurs propriétés de l'échantillon biologique.
PCT/US2024/047097 2023-09-18 2024-09-17 Système d'analyse d'échantillons biologiques Pending WO2025064425A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN202311062642 2023-09-18
IN202311062642 2023-09-18

Publications (1)

Publication Number Publication Date
WO2025064425A1 true WO2025064425A1 (fr) 2025-03-27

Family

ID=92932841

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/047097 Pending WO2025064425A1 (fr) 2023-09-18 2024-09-17 Système d'analyse d'échantillons biologiques

Country Status (1)

Country Link
WO (1) WO2025064425A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212094A (en) * 1986-09-16 1993-05-18 Kabushiki Kaisha Toshiba Automatic chemical analyzer
US7459713B2 (en) * 2003-08-14 2008-12-02 Microptix Technologies, Llc Integrated sensing system approach for handheld spectral measurements having a disposable sample handling apparatus
US20140118743A1 (en) * 2012-10-31 2014-05-01 Lite-On It Corporation Optical measuring apparatus and optical measuring method
EP2988111B1 (fr) * 2013-04-02 2021-11-03 Hitachi High-Tech Corporation Analyseur et analyseur automatique
CN215768617U (zh) * 2021-06-29 2022-02-08 迈克医疗电子有限公司 生化光电检测系统与生化分析仪

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5212094A (en) * 1986-09-16 1993-05-18 Kabushiki Kaisha Toshiba Automatic chemical analyzer
US7459713B2 (en) * 2003-08-14 2008-12-02 Microptix Technologies, Llc Integrated sensing system approach for handheld spectral measurements having a disposable sample handling apparatus
US20140118743A1 (en) * 2012-10-31 2014-05-01 Lite-On It Corporation Optical measuring apparatus and optical measuring method
EP2988111B1 (fr) * 2013-04-02 2021-11-03 Hitachi High-Tech Corporation Analyseur et analyseur automatique
CN215768617U (zh) * 2021-06-29 2022-02-08 迈克医疗电子有限公司 生化光电检测系统与生化分析仪

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZHANG MIN ET AL: "The current status and development of OD measurement technique", 20131219, vol. 9046, 19 December 2013 (2013-12-19), pages 904614 - 904614, XP060032307, DOI: 10.1117/12.2037509 *

Similar Documents

Publication Publication Date Title
CA2322895C (fr) Detecteur spectrophotometrique et nephelometrique
US8064061B2 (en) Sample analyzer and sample analyzing method
US4945250A (en) Optical read head for immunoassay instrument
EP1830174B1 (fr) Analyseur d'échantillon de fluorescence multicanal
US6836332B2 (en) Instrument and method for testing fluid characteristics
US6396584B1 (en) Pipette adapter, absorbance measuring pipette, tip, absorbance measuring apparatus, and absorbance measuring
JPH06343454A (ja) コンパクトな血液培養装置
US12352685B2 (en) Apparatus and method for measuring the light absorbance of a substance in a solution
AU738290B2 (en) Method and apparatus for determining characteristics of a sample in the presence of ambient light
US9285311B2 (en) System for performing scattering and absorbance assays
US4977325A (en) Optical read system and immunoassay method
JP7206570B2 (ja) 分析装置
JP2005147826A (ja) 蛍光測定装置
US20070037272A1 (en) System for optically analyzing a substance
JP2010503861A (ja) 光ファイバー励起子を使用する生化学アッセイ検出
CN110954486A (zh) 校正由实验室仪器中的检测单元的检测器测量的信号光强度的方法
CN103261858A (zh) 定量样品中的核酸
WO2022031458A1 (fr) Analyseur de spectroscopie d'absorbance et procédé d'utilisation
WO2025064425A1 (fr) Système d'analyse d'échantillons biologiques
WO2025236461A1 (fr) Système optique pour analyseur biochimique à sec
CN210664766U (zh) 一种单光源多检测模块光度计
RU70577U1 (ru) Планшетный микроколориметр
CN117169122A (zh) 生化分光系统和生化分析仪
KR20220126773A (ko) 기체와 부유물질을 검출하는 광학 검출기
HU198793B (hu) MÉRftEERENDEZÉS FOLYÉKONY KÖZEGEK FÉNYELNYELÉSÉNEK, ILLETVE EitNYSZÓRÁSÁNAK MEGHATÁROZÁSÁRA

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24783103

Country of ref document: EP

Kind code of ref document: A1