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WO2025063915A1 - Production of probiotic bacteria based biomaterial and bioplastic - Google Patents

Production of probiotic bacteria based biomaterial and bioplastic Download PDF

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Publication number
WO2025063915A1
WO2025063915A1 PCT/TR2023/051290 TR2023051290W WO2025063915A1 WO 2025063915 A1 WO2025063915 A1 WO 2025063915A1 TR 2023051290 W TR2023051290 W TR 2023051290W WO 2025063915 A1 WO2025063915 A1 WO 2025063915A1
Authority
WO
WIPO (PCT)
Prior art keywords
probiotic bacteria
probiotic
bacteria based
probif
bioplastic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/TR2023/051290
Other languages
French (fr)
Inventor
Hüseyin Sancar BOZKURT
Original Assignee
Bi̇oprobi̇f Sağlik Gida Arge Sanayi̇ Ve Ti̇caret Li̇mi̇ted Şi̇rketi̇
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from TR2023/011781 external-priority patent/TR2023011781A2/en
Application filed by Bi̇oprobi̇f Sağlik Gida Arge Sanayi̇ Ve Ti̇caret Li̇mi̇ted Şi̇rketi̇ filed Critical Bi̇oprobi̇f Sağlik Gida Arge Sanayi̇ Ve Ti̇caret Li̇mi̇ted Şi̇rketi̇
Publication of WO2025063915A1 publication Critical patent/WO2025063915A1/en
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L101/00Compositions of unspecified macromolecular compounds
    • C08L101/16Compositions of unspecified macromolecular compounds the macromolecular compounds being biodegradable
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135Bacteria or derivatives thereof, e.g. probiotics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66Microorganisms or materials therefrom
    • A61K35/74Bacteria
    • A61K35/741Probiotics
    • A61K35/744Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/745Bifidobacteria
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L23/00Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers
    • C08L23/02Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers not modified by chemical after-treatment
    • C08L23/04Homopolymers or copolymers of ethene

Definitions

  • the invention relates to the production of a probiotic bacteria based biomaterial and bioplastic, which can be used in the health, agriculture, food and industrial sectors, and which contributes significantly to a sustainable future by reducing the carbon ratio by compositing the probiotic bacteria based polymer material with a reduced proportion of petroleum-derived thermoplastic and biodegradable material.
  • probiotic bacteria based biomaterials and bioplastics offer numerous advantages:
  • Biodegradability These materials naturally degrade, reducing the plastic waste load in landfills and oceans.
  • Probiotic metabolites improve material properties, making them suitable for various applications.
  • Health Benefits Potential applications extend to the creation of functional materials that support human and environmental health
  • bio-based products lie in sustainability, lower carbon footprint, biodegradability, reduced toxicity and the potential to create different markets.
  • bio-based products By using renewable resources, bio-based products contribute to the preservation of ecosystems and minimize the depletion of finite resources. Their production also tends to emit less greenhouse gases and thus has a positive impact on climate change.
  • the probiotic bacteria based polymer material to be produced with the invention makes an important contribution to a sustainable future with its reduced petroleum- derived thermoplastic ratio and reduced carbon ratio by making it composite with biodegradable material.
  • the invention combines Probiotic Bifidobacterium infantis, Xylooligosaccharide and Sodium Alginate in materials known as "Probif".
  • Probif Linear Low Density Polyethylene
  • LLDPE Linear Low Density Polyethylene
  • Glycerol Monostearate the invention produces Probiotic Containing Bioplastics.
  • probiotic bacteria based biomaterials are as follows; in the (100 gr) material by weight;
  • LLDPE Linear Low Density Polyethylene
  • Probif Composite is based on Probif, a blend of Probiotic Bifidobacterium infantis, Xylooligosaccharide and Sodium Alginate. This composition gives the material not only biodegradability but also probiotic benefits, making it suitable for health and environmentally sensitive applications.
  • Glycerol in powder form is incorporated into the material. Beyond its plasticizer role, it contributes to the flexibility and durability of the material.
  • Linear Low Density Polyethylene (LLDPE) is in granular form and meticulously blended with Probif and Glycerol components. Variation in LLDPE concentration and specific processing parameters results in a range of material properties tailored to different applications.
  • Probiotic Containing Bioplastics is a multi-stage process utilizing a precise blend of biomaterials as follows:
  • Probif Addition The powdered Probif is meticulously blended into the formulation. Varying concentrations offer flexibility in adapting the probiotic content of the material.
  • Glycerol also in powder form, is added to the mixture. Combining it adds to the flexibility and strength of the material.
  • Extrusion and Granulation The mixture of Probif, Glycerol and LLDPE is mixed in bulk and extruded using an extruder (Extruder RTX-530). This process produces bioplastic granules using a temperature between 100°C and 170°C.
  • Granule Formation The extruded material is converted into granules, providing versatility for various applications.
  • Injection Molding The granules are molded into a multitude of products, from storage containers, bags and utensils to healthcare products such as sterile gloves, surgical supplies and syringes. Moreover, these bioplastics find applications in construction, agriculture, food processing and even the white goods industry.
  • Bioplastics with Probiotics is at the forefront of sustainable materials innovation, blending the latest biomaterials and precise production techniques.
  • This invention not only offers an environmentally conscious alternative to traditional plastics, but also offers probiotic benefits to a variety of industries, paving the way for a more sustainable, healthier future for both the environment and humanity. All technical and other features mentioned in each claim are followed by a reference number and these reference numbers are used only to facilitate understanding of the claims and should not be considered for illustrative purposes as limiting the scope of any of the elements indicated by these reference numbers.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Biological Depolymerization Polymers (AREA)

Abstract

The invention relates to the production of probiotic bacteria based biomaterial and bioplastic which is produced by extrusion consisting of Probif, Glycerol Monostearate and Linear Low Density Polyethylene (LLDPE) ExxonMobil LL 1001AV, a composite in powder form with unique properties and applications, and which provides for the creation of various products by molding using an injection device.

Description

PRODUCTION OF PROBIOTIC BACTERIA BASED BIOMATERIAL AND BIOPLASTIC
Technical Field
The invention relates to the production of a probiotic bacteria based biomaterial and bioplastic, which can be used in the health, agriculture, food and industrial sectors, and which contributes significantly to a sustainable future by reducing the carbon ratio by compositing the probiotic bacteria based polymer material with a reduced proportion of petroleum-derived thermoplastic and biodegradable material.
Background of the Invention
In a world increasingly focused on sustainability, a remarkable innovation is emerging at the intersection of biology and materials science. Probiotic Bacteria Based Biomaterial and Bioplastics combine the benefits of probiotic bacteria with biodegradable polymers, paving the way for a sustainable and thus environmentally friendly future.
Probiotic bacteria based biomaterials and bioplastics are materials that also provide benefits to the environment. At the heart of this technology is the synergy between beneficial probiotic microorganisms and biodegradable polymers derived from renewable resources such as starch, cellulose or polyhydroxyalkanoates (PHA).
The production journey of these innovative materials begins with the careful selection of probiotic bacteria strains known for their compatibility with biodegradable polymers. These strains are grown in sterile fermentation environments under controlled conditions of temperature, pH and oxygen levels. As they grow, they produce metabolites that enhance the properties of biodegradable polymers.
Once the probiotic cultures reach the desired state, they are incorporated into the biodegradable polymer matrix, ensuring even distribution and functional enhancement. Processing techniques such as extrusion or injection molding are used to shape the final biomaterials and bioplastics. Probiotic bacteria based biomaterials and bioplastics offer numerous advantages:
Biodegradability: These materials naturally degrade, reducing the plastic waste load in landfills and oceans.
Functional Properties: Probiotic metabolites improve material properties, making them suitable for various applications.
Sustainability: The use of renewable polymers and probiotics promotes environmentally friendly production.
Health Benefits: Potential applications extend to the creation of functional materials that support human and environmental health
Strict quality control and testing procedures ensure the viability, biodegradability and desired material properties of probiotic cultures. Microbiological analysis, biodegradability testing and mechanical evaluations are essential components of the quality assurance process.
Probiotic Bacteria Based Biomaterials and Bioplastics represent an important step forward in the search for a sustainable and environmentally friendly future. These materials have the potential to revolutionize industries from packaging to healthcare by offering a viable alternative to traditional plastics.
For a greener world, innovations in the production of biomaterials and bioplastics demonstrate the extraordinary possibilities that arise when nature's wisdom is used for the improvement of society and the planet. Probiotic bacteria based materials are not just a technological advance; they are a testament to the harmonious coexistence of science and nature, paving the way for a more sustainable and healthier future for all.
Currently used petroleum-derived food packaging has a negative impact on the environment and human health. Collecting and recycling these products is a major problem in terms of time and cost.
The main advantages of bio-based products lie in sustainability, lower carbon footprint, biodegradability, reduced toxicity and the potential to create different markets. By using renewable resources, bio-based products contribute to the preservation of ecosystems and minimize the depletion of finite resources. Their production also tends to emit less greenhouse gases and thus has a positive impact on climate change.
The probiotic bacteria based polymer material to be produced with the invention makes an important contribution to a sustainable future with its reduced petroleum- derived thermoplastic ratio and reduced carbon ratio by making it composite with biodegradable material.
The structural and characteristic features and all advantages of the invention will be more clearly understood with the detailed description below, and therefore this evaluation should be made in consideration of the detailed description.
Detailed Description of the Invention
In this detailed description, the invention is described with respect to the production of probiotic bacteria based biomaterial and bioplastic without any limiting effect.
The invention combines Probiotic Bifidobacterium infantis, Xylooligosaccharide and Sodium Alginate in materials known as "Probif". When integrated with Linear Low Density Polyethylene (LLDPE) and 90% Glycerol Monostearate, the invention produces Probiotic Containing Bioplastics.
The components used in the production of the invention probiotic bacteria based biomaterials are as follows; in the (100 gr) material by weight;
• - Probif in the range of 1 -30% (in powder form)
• - Glycerol in the range of 1 -10% (in powder form)
• - Linear Low Density Polyethylene (LLDPE) in the range of 1% - 60% (Granular)
Probif Composite is based on Probif, a blend of Probiotic Bifidobacterium infantis, Xylooligosaccharide and Sodium Alginate. This composition gives the material not only biodegradability but also probiotic benefits, making it suitable for health and environmentally sensitive applications.
Glycerol in powder form is incorporated into the material. Beyond its plasticizer role, it contributes to the flexibility and durability of the material.
Linear Low Density Polyethylene (LLDPE) is in granular form and meticulously blended with Probif and Glycerol components. Variation in LLDPE concentration and specific processing parameters results in a range of material properties tailored to different applications.
The production of Probiotic Containing Bioplastics is a multi-stage process utilizing a precise blend of biomaterials as follows:
Probif Addition: The powdered Probif is meticulously blended into the formulation. Varying concentrations offer flexibility in adapting the probiotic content of the material.
Glycerol Increase: Glycerol, also in powder form, is added to the mixture. Combining it adds to the flexibility and strength of the material.
Extrusion and Granulation: The mixture of Probif, Glycerol and LLDPE is mixed in bulk and extruded using an extruder (Extruder RTX-530). This process produces bioplastic granules using a temperature between 100°C and 170°C.
Granule Formation: The extruded material is converted into granules, providing versatility for various applications.
Injection Molding: The granules are molded into a multitude of products, from storage containers, bags and utensils to healthcare products such as sterile gloves, surgical supplies and syringes. Moreover, these bioplastics find applications in construction, agriculture, food processing and even the white goods industry.
Environmentally Friendly Packaging: With a focus on biodegradability, it is ideal for storage containers as well as bags, and reduces environmental impact.
Developments in Healthcare Services: Sterile and non-sterile gloves, surgical mesh materials and syringes provide additional health benefits by utilizing probiotic content.
Industrial Versatility: Their integration into plastic crates increases the efficiency of the agriculture and food industries, while their adaptability in construction and appliance manufacturing enhances sustainability.
Bioplastics with Probiotics is at the forefront of sustainable materials innovation, blending the latest biomaterials and precise production techniques. This invention not only offers an environmentally conscious alternative to traditional plastics, but also offers probiotic benefits to a variety of industries, paving the way for a more sustainable, healthier future for both the environment and humanity. All technical and other features mentioned in each claim are followed by a reference number and these reference numbers are used only to facilitate understanding of the claims and should not be considered for illustrative purposes as limiting the scope of any of the elements indicated by these reference numbers.
It is clear that a person skilled in the art can also demonstrate the novelty set forth in the invention by using similar embodiments and/or can apply this embodiment to other areas with similar purposes used in the relevant art. Therefore, it is also obvious that such embodiments will lack the criterion of novelty and especially the criterion of exceeding the state of the art.

Claims

1. Probiotic bacteria based biomaterial wherein it comprises the following materials in the (100 gr) material by weight;
• - Probif in the range of 1 -30% (in powder form)
• - Glycerol in the range of 1 -10% (in powder form)
• - Linear Low Density Polyethylene (LLDPE) in the range of 1 % - 60% (Granular)
2. Probiotic bacteria based biomaterial according to Claim 1 , wherein it comprises a Probif composite comprising Probiotic Bifidobacterium infantis, Xylooligosaccharide and Sodium alginate in a ratio optimized for probiotic activity and biodegradability.
3. Probiotic bacteria based biomaterial according to Claim 1 , wherein comprises Glycerol Monostearate, which contributes to the flexibility and elasticity of the bioplastic material.
4. Production of probiotic bacteria based biomaterial and bioplastic and characterized with the following process steps:
• Addition of probif in powder form, and addition of Glycerol Monostearate in powder form,
• The mixture of probif, glycerol and LLDPE was mixed in bulk and subjected to extrusion using Extruder device (Extruder RTX-530),
• obtaining bioplastic granules using temperatures between 100°C and 170°C in this process
SUBSTITUTE SHEET (RULE 26)
PCT/TR2023/051290 2023-09-21 2023-11-08 Production of probiotic bacteria based biomaterial and bioplastic Pending WO2025063915A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2023011781 2023-09-21
TR2023/011781 TR2023011781A2 (en) 2023-09-21 PROBIOTIC BACTERIA-BASED BIOMATERIALS AND BIOPLASTICS PRODUCTION

Publications (1)

Publication Number Publication Date
WO2025063915A1 true WO2025063915A1 (en) 2025-03-27

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Application Number Title Priority Date Filing Date
PCT/TR2023/051290 Pending WO2025063915A1 (en) 2023-09-21 2023-11-08 Production of probiotic bacteria based biomaterial and bioplastic

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160022592A1 (en) * 2013-03-14 2016-01-28 Therabiome, Llc Targeted gastrointestinal tract delivery of probiotic organisms and/or therapeutic agents
CN106366358A (en) * 2016-08-31 2017-02-01 迁安市隆赓塑料制品有限公司 Multifunctional polyethylene photo-biodegradable mulching film
WO2020264063A1 (en) * 2019-06-25 2020-12-30 Decomer Technology Inc. Hydro-liquid soluble films, products and uses thereof
CN112244302A (en) * 2020-11-04 2021-01-22 拉格比(广东)健康科技有限公司 Probiotic drop for improving infantile diarrhea
KR102300547B1 (en) * 2021-04-30 2021-09-13 주식회사 더자연 Biodegradable Plastic Composite using Livestock Organic Resource and Manufacturing Method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160022592A1 (en) * 2013-03-14 2016-01-28 Therabiome, Llc Targeted gastrointestinal tract delivery of probiotic organisms and/or therapeutic agents
CN106366358A (en) * 2016-08-31 2017-02-01 迁安市隆赓塑料制品有限公司 Multifunctional polyethylene photo-biodegradable mulching film
WO2020264063A1 (en) * 2019-06-25 2020-12-30 Decomer Technology Inc. Hydro-liquid soluble films, products and uses thereof
CN112244302A (en) * 2020-11-04 2021-01-22 拉格比(广东)健康科技有限公司 Probiotic drop for improving infantile diarrhea
KR102300547B1 (en) * 2021-04-30 2021-09-13 주식회사 더자연 Biodegradable Plastic Composite using Livestock Organic Resource and Manufacturing Method thereof

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