[go: up one dir, main page]

WO2025054146A3 - Label free analyte binder and labeled probe - Google Patents

Label free analyte binder and labeled probe Download PDF

Info

Publication number
WO2025054146A3
WO2025054146A3 PCT/US2024/045094 US2024045094W WO2025054146A3 WO 2025054146 A3 WO2025054146 A3 WO 2025054146A3 US 2024045094 W US2024045094 W US 2024045094W WO 2025054146 A3 WO2025054146 A3 WO 2025054146A3
Authority
WO
WIPO (PCT)
Prior art keywords
labeled probe
free analyte
label free
analyte binder
analyte binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/045094
Other languages
French (fr)
Other versions
WO2025054146A2 (en
Inventor
Min-Jui Richard Shen
Denis MALYSHEV
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eglint Inc
Original Assignee
Eglint Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eglint Inc filed Critical Eglint Inc
Publication of WO2025054146A2 publication Critical patent/WO2025054146A2/en
Publication of WO2025054146A3 publication Critical patent/WO2025054146A3/en
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N2021/757—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated using immobilised reagents

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Analyte binding to analyte binding moieties such as oligos on an array such as a surface is disclosed. Binding is assayed using, for example, fluorophore labeled probes rather than using fluorophores on the analyte binding moieties, resulting in a substantial increase in system efficiency.
PCT/US2024/045094 2023-09-08 2024-09-04 Label free analyte binder and labeled probe Pending WO2025054146A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363581326P 2023-09-08 2023-09-08
US63/581,326 2023-09-08

Publications (2)

Publication Number Publication Date
WO2025054146A2 WO2025054146A2 (en) 2025-03-13
WO2025054146A3 true WO2025054146A3 (en) 2025-06-12

Family

ID=94923991

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/045094 Pending WO2025054146A2 (en) 2023-09-08 2024-09-04 Label free analyte binder and labeled probe

Country Status (1)

Country Link
WO (1) WO2025054146A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150219652A1 (en) * 2013-11-29 2015-08-06 The Governors Of The University Of Alberta Binding-induced formation of dna three-way junctions from non-dna targets
US20180094316A1 (en) * 2000-02-07 2018-04-05 Illumina, Inc. Multiplex nucleic acid reactions
US20190376987A1 (en) * 2018-06-11 2019-12-12 The Florida International University Board Of Trustees Methods for generating structure-switching aptamers and uses thereof
US20210324374A1 (en) * 2018-06-04 2021-10-21 Chan Zuckerberg Biohub, Inc. Compositions and methods for screening aptamers

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180094316A1 (en) * 2000-02-07 2018-04-05 Illumina, Inc. Multiplex nucleic acid reactions
US20150219652A1 (en) * 2013-11-29 2015-08-06 The Governors Of The University Of Alberta Binding-induced formation of dna three-way junctions from non-dna targets
US20210324374A1 (en) * 2018-06-04 2021-10-21 Chan Zuckerberg Biohub, Inc. Compositions and methods for screening aptamers
US20190376987A1 (en) * 2018-06-11 2019-12-12 The Florida International University Board Of Trustees Methods for generating structure-switching aptamers and uses thereof

Also Published As

Publication number Publication date
WO2025054146A2 (en) 2025-03-13

Similar Documents

Publication Publication Date Title
MXPA03001879A (en) Method and system for detecting nucleic acids.
GB0308851D0 (en) Method
CA2337762A1 (en) Metal ion-binding mass markers for nucleic acids
WO2005074467A3 (en) Matrix array nanobiosensor
WO2001027624A3 (en) Compositions and methods for detecting protein modification and enzymatic activity
CA2304260A1 (en) Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method
CA2377685A1 (en) Automatic barcode creation for data transfer and retrieval
EP2312317A3 (en) Fluorescent detection system and dye set for use therewith
AU3062300A (en) Methods for detecting changes to cellular components
CA2170592A1 (en) Method for Activation of Polyanionic Fluorescent Dyes in Low Dielectric Media with Quaternary Onium Compounds
CA2399368A1 (en) Fluorescent detection method and reagent
WO2007035929A3 (en) Method for detecting and quantitating multiple subcellular components
WO2025054146A3 (en) Label free analyte binder and labeled probe
WO2001038585A3 (en) Polymer arrays and methods of using labeled probe molecules to identify and quantify target molecule expression
AU3414599A (en) Detection method
AU2002361146A1 (en) Normalisation of microarray data based on hybridisation with an internal reference
WO2003064608A3 (en) Surface based translation system
EP1249503A3 (en) Multiplexed gene analysis on a mobile solid support
CA2407760A1 (en) Methods for diagnosis of low grade astrocytoma
WO2006036735A3 (en) Method for detecting and quantitating multiple subcellular components
CN219608936U (en) Magnaporthe grisea nucleic acid colloidal gold test strip based on RAA isothermal amplification reagent
CN109491067B (en) 10-time working distance objective lens of large-field microscope
WO2003002759A3 (en) Detection of specific dinucleotides in dna-samples by fluorescence resonance energy transfer (fret)
EP1229318A3 (en) Chromatic dispersion distribution measurement apparatus, method and storage medium for the same
ATE383579T1 (en) REDUCING FLUORESCENCE QUENCHMENT DURING A DETERMINATION

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24863499

Country of ref document: EP

Kind code of ref document: A2