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WO2025052145A3 - Enzymes et procédés - Google Patents

Enzymes et procédés Download PDF

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Publication number
WO2025052145A3
WO2025052145A3 PCT/GB2024/052336 GB2024052336W WO2025052145A3 WO 2025052145 A3 WO2025052145 A3 WO 2025052145A3 GB 2024052336 W GB2024052336 W GB 2024052336W WO 2025052145 A3 WO2025052145 A3 WO 2025052145A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzymes
methods
tdt
seq
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/GB2024/052336
Other languages
English (en)
Other versions
WO2025052145A2 (fr
Inventor
Raquel Maria Sanches-Kuiper
Stephane Patrick EMOND
Laurens Herman LINDENBURG
David Robert Watson HODGSON
Pascal LANG
Zhengyun ZHAO
Edward Andrew NESBITT
Matthew James Hayes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evonetix Ltd
Original Assignee
Evonetix Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonetix Ltd filed Critical Evonetix Ltd
Publication of WO2025052145A2 publication Critical patent/WO2025052145A2/fr
Publication of WO2025052145A3 publication Critical patent/WO2025052145A3/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne une désoxynucléotidyl transférase terminale (TdT) comprenant une séquence d'acides aminés qui présente au moins 75 % d'identité de séquence avec SEQ ID NO : 3.
PCT/GB2024/052336 2023-09-08 2024-09-06 Enzymes et procédés Pending WO2025052145A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB2313749.0 2023-09-08
GB2313749.0A GB2635104A (en) 2023-09-08 2023-09-08 Enzymes and methods

Publications (2)

Publication Number Publication Date
WO2025052145A2 WO2025052145A2 (fr) 2025-03-13
WO2025052145A3 true WO2025052145A3 (fr) 2025-05-15

Family

ID=88412836

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2024/052336 Pending WO2025052145A2 (fr) 2023-09-08 2024-09-06 Enzymes et procédés

Country Status (2)

Country Link
GB (1) GB2635104A (fr)
WO (1) WO2025052145A2 (fr)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019135007A1 (fr) * 2018-01-08 2019-07-11 Dna Script Variants de la désoxynucléotidyle transférase terminale et leurs utilisations
WO2020099451A1 (fr) * 2018-11-14 2020-05-22 Dna Script Variants de désoxynucléotidyltransférase et utilisations correspondantes
US20210009969A1 (en) * 2014-10-20 2021-01-14 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
WO2022013094A1 (fr) * 2020-07-15 2022-01-20 Dna Script Synthèse enzymatique massivement parallèle de polynucléotides
WO2022063835A1 (fr) * 2020-09-22 2022-03-31 Dna Script Variants stabilisés de désoxynucléotidyl transférase terminale tronqués à l'extrémité n-terminale et utilisations associées
WO2023069865A1 (fr) * 2021-10-15 2023-04-27 Codexis, Inc. Variants de désoxynucléotidyl transférase terminale modifiée

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201901501D0 (en) * 2019-02-04 2019-03-27 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
US20230159903A1 (en) * 2020-04-20 2023-05-25 Dna Script Terminal Deoxynucleotidyl Transferase Variants and Uses Thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210009969A1 (en) * 2014-10-20 2021-01-14 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
WO2019135007A1 (fr) * 2018-01-08 2019-07-11 Dna Script Variants de la désoxynucléotidyle transférase terminale et leurs utilisations
WO2020099451A1 (fr) * 2018-11-14 2020-05-22 Dna Script Variants de désoxynucléotidyltransférase et utilisations correspondantes
WO2022013094A1 (fr) * 2020-07-15 2022-01-20 Dna Script Synthèse enzymatique massivement parallèle de polynucléotides
WO2022063835A1 (fr) * 2020-09-22 2022-03-31 Dna Script Variants stabilisés de désoxynucléotidyl transférase terminale tronqués à l'extrémité n-terminale et utilisations associées
WO2023069865A1 (fr) * 2021-10-15 2023-04-27 Codexis, Inc. Variants de désoxynucléotidyl transférase terminale modifiée

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JASMINE PUAY SUAN CHUA ET AL: "Evolving a Thermostable Terminal Deoxynucleotidyl Transferase", ACS SYNTHETIC BIOLOGY, vol. 9, no. 7, 5 June 2020 (2020-06-05), Washington DC ,USA, pages 1725 - 1735, XP055763199, ISSN: 2161-5063, DOI: 10.1021/acssynbio.0c00078 *
SEBASTIAN BARTHEL ET AL: "Enhancing Terminal Deoxynucleotidyl Transferase Activity on Substrates with 3' Terminal Structures for Enzymatic De Novo DNA Synthesis", GENES, vol. 11, no. 1, 16 January 2020 (2020-01-16), pages 102, XP055763206, DOI: 10.3390/genes11010102 *

Also Published As

Publication number Publication date
GB2635104A (en) 2025-05-07
GB202313749D0 (en) 2023-10-25
WO2025052145A2 (fr) 2025-03-13

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