[go: up one dir, main page]

WO2025048720A1 - Microscope confocal à balayage parallèle et procédé de microscopie confocale à balayage parallèle - Google Patents

Microscope confocal à balayage parallèle et procédé de microscopie confocale à balayage parallèle Download PDF

Info

Publication number
WO2025048720A1
WO2025048720A1 PCT/SG2024/050549 SG2024050549W WO2025048720A1 WO 2025048720 A1 WO2025048720 A1 WO 2025048720A1 SG 2024050549 W SG2024050549 W SG 2024050549W WO 2025048720 A1 WO2025048720 A1 WO 2025048720A1
Authority
WO
WIPO (PCT)
Prior art keywords
port
light
scanning confocal
parallel scanning
optical
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/SG2024/050549
Other languages
English (en)
Inventor
Poongkulali D/O RAJARAHM
Baocheng Li
Malini Carolene Devapiriyai OLIVO
Ruochong ZHANG
Yi QI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agency for Science Technology and Research Singapore
Original Assignee
Agency for Science Technology and Research Singapore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency for Science Technology and Research Singapore filed Critical Agency for Science Technology and Research Singapore
Publication of WO2025048720A1 publication Critical patent/WO2025048720A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/0048—Scanning details, e.g. scanning stages scanning mirrors, e.g. rotating or galvanomirrors, MEMS mirrors

Definitions

  • Various embodiments relate to a parallel scanning confocal microscope and a parallel scanning confocal microscopy method.
  • LSCM Laser scanning confocal microscopy
  • a parallel scanning confocal microscope may include an optical circulator including a first port, a second port and a third port; a light source optically coupled to the first port of the optical circulator; an optical arrangement in optical communication with the second port of the optical circulator; an focusing lens in optical communication with the optical arrangement; and a photodetector optically coupled to the third port of the optical circulator.
  • the light source may be configured to emit light towards the optical arrangement through the first port and the second port along an illumination path.
  • the optical arrangement may be configured to direct the light as multiple optical beams towards the focusing lens along the illumination path.
  • a parallel scanning confocal microscopy method may include emitting light through a first port and a second port of an optical circulator along an illumination path, directing the light as multiple optical beams along the illumination path, focusing the multiple optical beams into multiple illumination points to confocally and simultaneously illuminate a sample under observation along the illumination path, collecting the light reflected from the sample confocally along a reflection path, and directing the reflected light in a form of reflected photons through the second port and a third port of the optical circulator along the reflection path.
  • FIG. 1 shows a schematic representative view of a parallel scanning confocal microscope (PSCM), according to various embodiments.
  • PSCM parallel scanning confocal microscope
  • FIG. 2 shows a flow chart illustrating a parallel scanning confocal microscopy method, according to various embodiments.
  • FIG. 4 shows a schematic representation illustrating a PSCM with a second parallel scanning scheme, according to one example.
  • FIG. 5A shows a schematic cross-sectional view of a collimator with three fibers collimated together in close proximity used in the PSCM of FIG. 4, according to one example.
  • FIG. 5B shows a schematic cross-sectional view of a collimator with four fibers collimated together in close proximity used in the PSCM of FIG. 4, according to a different example.
  • FIG. 6A shows an image of resolution test targets used by the United States air force, according to an example.
  • FIG. 6B shows an image of a specific part of the resolution test targets of FIG. 6A, obtained by the PSCM.
  • FIG. 7 shows an image of red blood cells from human blood sample, as obtained by the PSCM.
  • FIG. 8 shows an image of white blood cells from human blood sample, as obtained by the PSCM.
  • Embodiments described in the context of one of the methods or devices are analogously valid for the other methods or devices. Similarly, embodiments described in the context of a method are analogously valid for a device, and vice versa.
  • the articles “a”, “an” and “the” as used with regard to a feature or element include a reference to one or more of the features or elements.
  • the phrase “at least” may include “exactly” and a reasonable variance.
  • the term “about”, or interchangeably “approximately”, as applied to a numeric value encompasses the exact value and a reasonable variance.
  • Various embodiments may provide a fiber-optic parallel scanning confocal microscope (PSCM) with single-pixel detector.
  • PSCM fiber-optic parallel scanning confocal microscope
  • the PSCM is miniaturized and may include light source, e.g. laser diodes or light emitting diodes (LEDs) with driver boards, single-mode or multi-mode fibers, fiber collimators, objective, galvo mirror or spatial light modulator (SLM), as well as single-pixel photodetectors.
  • light source e.g. laser diodes or light emitting diodes (LEDs) with driver boards, single-mode or multi-mode fibers, fiber collimators, objective, galvo mirror or spatial light modulator (SLM), as well as single-pixel photodetectors.
  • fiber circulators are employed to combine illumination, collection and detection ports. Attributing to the stability and flexibility of the circulators, the PSCM has a more robust confocal light path.
  • the PSCM may operate with two different novel scanning schemes, namely parallel scanning or simultaneous multiple point scanning, to improve imaging speed by significantly reducing the image acquisition time.
  • the resolutions may be as high as sub-micron levels, which make the PSCM suitable for real-time applications in life science, semiconductor, dermatology, material, amongst others.
  • FIG. 1 shows a schematic representative view of a parallel scanning confocal microscope 100, according to various embodiments.
  • the parallel scanning confocal microscope 100 may include an optical circulator 102 including a first port 104, a second port 106 and a third port 108; a light source 110 optically coupled (as denoted by a line 118) to the first port 104 of the optical circulator 102; an optical arrangement 112 in optical communication (as denoted by a line 120) with the second port 106 of the optical circulator 102; a focusing lens 114 in optical communication (as denoted by a line 122) with the optical arrangement 112; and a photodetector 116 optically coupled (as denoted by a line 124) to the third port 108 of the optical circulator 102.
  • the light source 110 may be configured to emit light towards the optical arrangement 1 12 through the first port 104 and the second port 106 along an illumination path.
  • the optical arrangement 112 may be configured to direct the light as multiple optical beams towards the focusing lens 114 along the illumination path.
  • the focusing lens 114 may be configured to focus the multiple optical beams into multiple illumination points to confocally and simultaneously illuminate a sample under observation (not shown in FIG. 1) by the parallel scanning confocal microscope 100 along the illumination path.
  • the focusing lens 1 14 may further be configured to collect the light reflected from the sample confocally along a reflection path.
  • the optical arrangement 112 may further be configured to direct the reflected light towards the photodetector 116 in a form of reflected photons through the second port 106 and the third port 108 along the reflection path.
  • the term “coupled” may mean connected to or in communication with, directly or indirectly.
  • the focusing lens 114 may be any type of focusing lens, including objectives (objective lens), convex lenses, metasurfaces, and the like.
  • the photodetector 116 may be a single -pixel photodetector. In other embodiments, the photodetector 116 may include array detectors.
  • the light source 1 10 may include a non-coherent light source, preferably a light emitting diode (LED).
  • a non-coherent light source preferably a light emitting diode (LED).
  • the parallel scanning confocal microscope 100 may essentially involve singlewavelength light source(s) and mechanical scanning. No spatial disperser may be required since spectral information is not considered. The setup of the parallel scanning confocal microscope 100 may also be much simpler without spectral or time encoding/decoding.
  • the optical arrangement 112 may be a single spatial light modulator (SLM) configured to modulate the light in forming the multiple optical beams along the illumination path.
  • the parallel scanning confocal microscope 100 may further include a data acquisition unit in electrical communication with the photodetector 116.
  • the data acquisition unit may be configured to acquire and process information based on the reflected photons to generate a reconstructed image of the sample.
  • the single spatial light modulator may be configured to generate a plurality of time-varying coded patterns based on the light reflected from the sample at pre-determined sampling intervals.
  • the photodetector 1 16 may be configured to detect the reflected photons including correlated light intensities between each time-varying coded pattern of the plurality of time-varying coded patterns and a target image of the sample. More specifically, each time-varying coded pattern of the plurality of time-varying coded patterns may form a row of an encoded matrix F, the target image may be represented by a target matrix having a plurality of elements, each element of the target matrix representing a light intensity at a location on the sample and the target matrix being expanded to a vector X.
  • This embodiment provides an implementation scheme of parallel scanning to improve the imaging speed significantly by employing structural illumination using a spatial light modulator and compressive sensing-based image reconstruction.
  • the image reconstruction may be carried out with a specific decoding algorithm.
  • compressive sensing (or minimization) algorithms may include but are not limited to off-the-shelf algorithm, orthogonal matching pursuit, ridge regression, gradient projection for sparse reconstruction, Nestorov’s algorithm.
  • the parallel scanning confocal microscope 100 may further include one or more additional optical circulators, each including a first port, a second port and a third port; and one or more additional photodetectors, each optically coupled to the third port of each of the one or more additional optical circulators.
  • the light source 110 may include multiple output ports, each output port optically coupled to the first port of each of the one or more additional optical circulators.
  • the optical arrangement 112 may further be in optical communication with the second port of each of the one or more additional optical circulators.
  • the optically coupled output port may be configured to emit light towards the optical arrangement 1 12 through the first port (of each additional optical circulator) and the second port (of each additional optical circulator) along the illumination path.
  • the optical arrangement 112 may further be configured to direct the reflected light towards the optically coupled additional photodetector through the second port (of each additional optical circulator) and the third port (of each additional optical circulator), along the reflection path, forming a part of the reflected photons.
  • the light source 1 10 may include a light emitting [0035]
  • the parallel scanning confocal microscope 100 may further include one or more additional optical circulators, each including a first port, a second port and a third port; one or more additional light sources, each optically coupled to the first port of each of the one or more additional optical circulators; and one or more additional photodetectors, each optically coupled to the third port of each of the one or more additional optical circulators.
  • the optical arrangement 112 may further be in optical communication with the second port of each of the one or more additional optical circulators.
  • the optically coupled additional light source may be configured to emit light towards the optical arrangement 112 through the first port and the second port along the illumination path.
  • the optical arrangement 1 12 may further be configured to direct the reflected light towards the optically coupled additional photodetector through the second port (of each additional optical circulator) and the third port (of each additional optical circulator), along the reflection path, forming a part of the reflected photons.
  • the light source 110 and the one or more additional light sources may be configured to emit the light simultaneously.
  • the light source and the one or more additional light sources each may include a light emitting diode (LED) or a laser diode.
  • each additional optical circulator, each additional photodetector and each additional light source may be respectively described in similar context with the optical circulator 102, the photodetector 116 and the light source 110 of FIG. I.
  • the optical arrangement 112 may include a collimator configured to collimate the light from or to de-collimate the reflected lighted to the second port of each of the optical circulator and the one or more additional optical circulators; and one or more scanning mirrors in optical communication with the collimator.
  • the one or more scanning mirrors may include one or more galvo mirrors, one or more micro-electrical-mechanical system (MEMS) mirrors, or one or more polygon scanning mirrors.
  • MEMS micro-electrical-mechanical system
  • the parallel scanning confocal microscope 100 may further include a data acquisition unit in electrical communication with the photodetector 116 and the one or more additional photodetectors.
  • the data acquisition unit may be configured to acquire and process information based on the reflected photons to generate an image of the sample.
  • the one or more additional photodetectors may include one or more additional single-pixel photodetectors, or one or more additional array detectors.
  • the abovementioned two preceding embodiments provide an implementation scheme of parallel scanning to improve the imaging speed significantly by employing multi-foci confocal parallel scanning with customized fiber collimator.
  • the implementation scheme of parallel scanning may be used for single-wavelength imaging. It provides N illumination points simultaneously and may reduce imaging time to 1/N. With this configuration, the need for image reconstruction may be avoided.
  • FIG. 2 shows a flow chart illustrating a parallel scanning confocal microscopy method 240, according to various embodiments.
  • light may be emitted through a first port (e.g. 104, FIG. 1 ) and a second port (e.g. 106, FIG. 1 ) of an optical circulator (e.g. 102, FIG. 1) along an illumination path.
  • the light may be directed as multiple optical beams along the illumination path.
  • the multiple optical beams may be focused into multiple illumination points to confocally and simultaneously illuminate a sample under observation along the illumination path.
  • the light reflected from the sample may be collected confocally along a reflection path.
  • the reflected light may be directed in a form of reflected photons through the second port and a third port (e.g. 108, FIG. 1 ) of the optical circulator along the reflection path.
  • the parallel scanning confocal microscopy method 240 may include the same or like elements or components as used in the parallel scanning confocal microscope 100 of FIG. 1, and as such, the like elements may be as described in the context of the parallel scanning confocal microscope 100 of FIG. 1, and therefore some corresponding descriptions are omitted here.
  • the method 240 may further include acquiring and processing information based on the reflected photons to generate an image of the sample.
  • directing the light (at Step 244) as multiple optical beams along the illumination path may include modulating the light and forming the multiple optical beams along the illumination path.
  • the method 240 may further include generating a plurality of time-varying coded patterns based on the light reflected from the sample at pre-determined sampling intervals.
  • the method 240 may further include detecting, at each pre-determined sampling interval, the reflected photons.
  • the reflected photons may include correlated light intensities between each time-varying coded pattern of the plurality of time-varying coded patterns and a target image of the sample.
  • Each time-varying coded pattern of the plurality of time-varying coded patterns forms a row of an encoded matrix F.
  • the target image may be represented by a target matrix having a plurality of elements, each element of the target matrix representing a light intensity at a location on the sample and the target matrix being expanded to a vector X.
  • the method 240 may further include performing compressive sensing.
  • 0 may be reconstructed through a minimization algorithm.
  • the minimization algorithm may include a convex optimization algorithm or a greedy algorithm.
  • the minimization algorithm may be defined by: minimize
  • the method 240 may further include providing one or more additional optical circulators, each including a first port, a second port and a third port; emitting light through the first port and the second port of each of the one or more additional optical circulators along the illumination path; and directing the reflected light through the second port and the third port of each of the one or more additional optical circulators, along the reflection path, forming a part of the reflected photons.
  • Emitting the light through the first port and the second port of each of the one or more additional optical circulators and emitting the light through the first port and the second port of the optical circulator (Step 242) may be carried out simultaneously.
  • Directing the light as multiple optical beams along the illumination path may include collimating the light from the second port of each of the optical circulator and the one or more additional optical circulators.
  • Directing the reflected light along the reflection path may include de-collimating the reflected lighted to the second port of each of the optical circulator and the one or more additional optical circulators. This configuration may avoid the need for reconstruction of the image.
  • Two schemes of parallel scanning confocal microscope (PSCM) based on fiber circulator may be provided.
  • PSDCM parallel scanning confocal microscope
  • the feature of fiber circulator is that light source goes into port 1 and travels to port 2, then from port 2 to port 3. Light cannot travel from port 1 to port 3 directly, bypassing port 2, which minimizes the interference from the light source.
  • the reconstructed accuracy may be ensured by minimizing the h norm function of the measured signals and the signals resulting from the predicted signals, and the li norm guarantees the sparse solution to satisfy the mentioned assumption.
  • An image 336 may be reconstructed by exploring its sparsity in the pixel domain using the minimization of the total variation (TV) or the total curvature (TC) of the images, at least Af-coded patterns 332a, 332b, 332c may be needed to fully recover/reconstruct the image 336, where N>M>0.25N (Vis pixel numbers of an image). Hence, imaging time of [(N-M) x sampling rate] may be saved.
  • FIG. 4 shows a schematic representation illustrating a PSCM 400 with a second parallel scanning scheme, according to one example.
  • the PSCM 400 may include the same or like elements or components as those of the parallel scanning confocal microscope 100 of FIG. 1 , and as such, the same ending numerals are assigned and the like elements may be as described in the context of the parallel scanning confocal microscope 100 of FIG. 1, and therefore the corresponding descriptions are omitted here.
  • FIG. 5 A shows a schematic cross-sectional view of the collimator 438 with three fibers 437 collimated together in close proximity, according to one example.
  • the three fibers 437 accomodated by the the collimator 438 may be arranged vertically or along a same plane with one another.
  • FIG. 5B shows a schematic cross-sectional view of a collimator 438’ with four fibers 437’ collimated together in close proximity, according to a different example.
  • the four fibers 437’ accomodated by the the collimator 438’ may be arranged in an array form.
  • Other examples (not shown in FIGS. 5A and 5B) with various number of fibers collimated by the collimator as well as different arrangments of each of the fibers with respect to an adjacent fiber within the collimator may be appreciated.
  • the two or more light beams 413 from the collimator 438 may be directed onto the sample 432 through an objective 414 by a galvo 439, forming two or more focus points 419 on the sample 432.
  • the objective 414 may be coupled to a z-axis motor 430 to facilitate z-axis adjustments.
  • the reflected photons may be collected by the same objective 414 in a confocal way and reflect to the original point of the collimator 438, then transmitted to Port 3 408a, 408b, 408c through individual fiber circulators 402a, 402b, 402c. Photons collected by the photodetectors 416a, 416b, 416c may be analysed in a DAQ 426.
  • the two or more focus points 419 may enable parallel scanning of the sample 432.
  • the imaging time may be shortened by two or more times and no complex algorithm may be needed for the construction of image.
  • the number of parallel scanning points may be highly customizable, based on different applications. In general, more parallel scanning points provides faster imaging speed but with higher system cost. [0066] Preliminary trials were conducted to evaluate the performance of the PSCM (e.g. 300, 400 of FIGS. 3 and 4).
  • FIG. 6A shows an image of resolution test targets 601 used by the United States air force, according to an example.
  • FIG. 6B show an image 603 of a specific part of the resolution test targets 601 of FIG. 6A, obtained by the PSCM 300 with the first parallel scanning scheme.
  • the calculated lateral resolution is about 462 nm.
  • FIG. 7 shows an image 705 of red blood cells from human blood sample, as obtained by the PSCM 300 with the first parallel scanning scheme.
  • FIG. 8 shows an image 807 of white blood cells from human blood sample, as obtained by the PSCM 300 with the first parallel scanning scheme.
  • the preliminary trials endorse the performances of the PSCM, which is miniaturized, robust and based on the use of fiber circulator with minimal cross interference.
  • a majority of free-space optical components may be removed by using fiber circulator and fiber bundles.
  • Using fiber circulator instead of fiber combiner/coupler advantageously improves signal quality and minimize interference, while achieving high speed parallel scanning through fiber components.

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

Des modes de réalisation de la présente invention concernent un microscope confocal à balayage parallèle. Le microscope comprend un circulateur optique comprenant un premier port, un deuxième port et un troisième port ; une source de lumière couplée optiquement au premier port ; un agencement optique en communication optique avec le deuxième port ; une lentille de focalisation ; et un photodétecteur couplé optiquement au troisième port. La source de lumière peut être configurée pour émettre de la lumière vers l'agencement optique le long d'un trajet d'éclairage. La lumière peut être dirigée sous la forme de multiples faisceaux optiques vers la lentille de focalisation, qui peut être configurée pour focaliser les faisceaux pour éclairer de manière conique et simultanée un échantillon le long du trajet d'éclairage ; et pour collecter la lumière réfléchie par l'échantillon de manière conique le long d'un trajet de réflexion. La lumière réfléchie peut être dirigée vers le photodétecteur le long du trajet de réflexion. Selon d'autres modes de réalisation, l'invention concerne également un procédé de microscopie confocale à balayage parallèle.
PCT/SG2024/050549 2023-08-29 2024-08-27 Microscope confocal à balayage parallèle et procédé de microscopie confocale à balayage parallèle Pending WO2025048720A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG10202302433R 2023-08-29
SG10202302433R 2023-08-29

Publications (1)

Publication Number Publication Date
WO2025048720A1 true WO2025048720A1 (fr) 2025-03-06

Family

ID=94820538

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SG2024/050549 Pending WO2025048720A1 (fr) 2023-08-29 2024-08-27 Microscope confocal à balayage parallèle et procédé de microscopie confocale à balayage parallèle

Country Status (1)

Country Link
WO (1) WO2025048720A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070081236A1 (en) * 2005-09-29 2007-04-12 The General Hospital Corporation Method and apparatus for optical imaging via spectral encoding
US20110137178A1 (en) * 2009-10-06 2011-06-09 The General Hospital Corporation Devices and methods for imaging particular cells including eosinophils
US20120190928A1 (en) * 2011-01-24 2012-07-26 Boudoux Caroline Double clad fiber coupler and device
CN104568777A (zh) * 2015-01-12 2015-04-29 南京理工大学 基于频谱编码的共焦显微成像装置及方法
CN113946044A (zh) * 2021-09-09 2022-01-18 深圳大学 基于点扩散函数工程的多焦点多光子显微成像系统及方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070081236A1 (en) * 2005-09-29 2007-04-12 The General Hospital Corporation Method and apparatus for optical imaging via spectral encoding
US20110137178A1 (en) * 2009-10-06 2011-06-09 The General Hospital Corporation Devices and methods for imaging particular cells including eosinophils
US20120190928A1 (en) * 2011-01-24 2012-07-26 Boudoux Caroline Double clad fiber coupler and device
CN104568777A (zh) * 2015-01-12 2015-04-29 南京理工大学 基于频谱编码的共焦显微成像装置及方法
CN113946044A (zh) * 2021-09-09 2022-01-18 深圳大学 基于点扩散函数工程的多焦点多光子显微成像系统及方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NARUSE, M. ET AL.: "Parallel confocal laser microscope system using smart pixel arrays", SPIE PROCEEDINGS NOVEL OPTICAL SYSTEMS DESIGN AND OPTIMIZATION III, vol. 4092, 2 October 2000 (2000-10-02), pages 94 - 101, XP008021621, [retrieved on 20241029], DOI: 10.1117/12.402414 *
ZHANG, Y. ET AL.: "Parallel large-range scanning confocal microscope based on a digital micromirror device", OPTIK, vol. 124, no. 13, 4 August 2012 (2012-08-04), pages 1585 - 1588, XP028538274, [retrieved on 20241029], DOI: 10.1016/J.IJLEO. 2012.04.02 8 *

Similar Documents

Publication Publication Date Title
EP2653091B1 (fr) Endoscope de balayage
US10151907B2 (en) Full-color three-dimennsional optical sectioning microscopic imaging system and method based on structured illumination
KR100940435B1 (ko) 2차원 광섬유 스캐닝 모듈, 이를 구비하는 광섬유 스캐닝 시스템 및 방법
US5784098A (en) Apparatus for measuring three-dimensional configurations
KR102588057B1 (ko) 광학 시스템 및 방법
KR101502236B1 (ko) 3 차원 다색 형광 공초점 현미경 및 이를 사용하는 시편의 깊이의 정보를 생성하는 방법
CN104634766B (zh) 一种基于泵浦‑探针技术的超分辨装置和方法
US20140088438A1 (en) Apparatus and method for fluorescence imaging and tomography using spatially structured illumination
JP6000010B2 (ja) レーザー走査型顕微鏡
US9816868B2 (en) Device for measuring temperature distribution
KR101541610B1 (ko) 공간 필터 기반 구조 조명 현미경
KR20210093245A (ko) 다중 모드 도파관 이미징
JPWO2018124285A1 (ja) イメージング装置及び方法
US20210018744A1 (en) Devices and methods for conveying and controlling light beams for lensless endo-microscopic imagery
US20220061644A1 (en) Holographic endoscope
JP2019503516A (ja) 光ファイバーのバンドルを使用した高分解能撮像のためのシステム及び方法
JP3816624B2 (ja) 三次元計測装置
JP2016202360A (ja) 撮像装置
US4634857A (en) Imaging arrangement for scanning using stationary optics
CN107430270A (zh) 光扫描装置的扫描轨迹测定方法、扫描轨迹测定装置和图像校准方法
WO2025048720A1 (fr) Microscope confocal à balayage parallèle et procédé de microscopie confocale à balayage parallèle
RU2579640C1 (ru) Конфокальный спектроанализатор изображений
US9007572B2 (en) Multifunction solid-state inspection system
Riza Coded access optical sensor (CAOS) imager and applications
KR102198254B1 (ko) 패턴 부호화를 이용한 광 간섭 단층 촬영 장치

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24860581

Country of ref document: EP

Kind code of ref document: A1