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WO2024236366A3 - Design and method of generating scarless circular rna - Google Patents

Design and method of generating scarless circular rna Download PDF

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Publication number
WO2024236366A3
WO2024236366A3 PCT/IB2024/000261 IB2024000261W WO2024236366A3 WO 2024236366 A3 WO2024236366 A3 WO 2024236366A3 IB 2024000261 W IB2024000261 W IB 2024000261W WO 2024236366 A3 WO2024236366 A3 WO 2024236366A3
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WO
WIPO (PCT)
Prior art keywords
segment
rna
scarless
generating
design
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/IB2024/000261
Other languages
French (fr)
Other versions
WO2024236366A2 (en
Inventor
Monalisa Chatterji
Anirudh RANGANATHAN
Srivatsa DWARAKANATH
Anand Prakash Khedkar
Asma Nadhira Haseen MEERA MOHAIDEEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekkei Bio Private Ltd
Original Assignee
Sekkei Bio Private Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sekkei Bio Private Ltd filed Critical Sekkei Bio Private Ltd
Publication of WO2024236366A2 publication Critical patent/WO2024236366A2/en
Publication of WO2024236366A3 publication Critical patent/WO2024236366A3/en
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/124—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes based on group I or II introns
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/532—Closed or circular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of making circularization of an RNA of interest without a use of permuted intron-exon (PIE) includes: providing a DNA (deoxyribonucleic acid) construct, wherein the DNA construct includes a Group I intron-based ribozyme, a sequence of interest, and a target sequence; causing an RNA to be transcribed from the DNA construct, the RNA includes a segment S1 corresponding to the ribozyme, a segment S2 corresponding to the target sequence, and a segment S3 corresponding to the sequence of interest; and effecting a base-pairing between the segment S1 and the segment S2, wherein an occurrence of the base-pairing induces circularization of the segment S3.
PCT/IB2024/000261 2023-05-12 2024-05-09 Design and method of generating scarless circular rna Pending WO2024236366A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN202341033449 2023-05-12
IN202341033449 2023-05-12

Publications (2)

Publication Number Publication Date
WO2024236366A2 WO2024236366A2 (en) 2024-11-21
WO2024236366A3 true WO2024236366A3 (en) 2024-12-26

Family

ID=91829081

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2024/000261 Pending WO2024236366A2 (en) 2023-05-12 2024-05-09 Design and method of generating scarless circular rna

Country Status (1)

Country Link
WO (1) WO2024236366A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018237372A1 (en) * 2017-06-23 2018-12-27 Cornell University RNA MOLECULES, METHODS FOR PRODUCING CIRCULAR RNA, AND METHODS OF TREATMENT
WO2020219563A1 (en) * 2019-04-22 2020-10-29 TCR2 Therapeutics Inc. Compositions and methods for tcr reprogramming using fusion proteins
WO2022247943A1 (en) * 2021-05-28 2022-12-01 Shanghai Circode Biomed Co., Ltd. Constructs and methods for preparing circular rnas and use thereof
EP4116421A1 (en) * 2021-03-10 2023-01-11 Rznomics Inc. Self-circularized rna structure
WO2023046153A1 (en) * 2021-09-26 2023-03-30 Center For Excellence In Molecular Cell Science, Chinese Academy Of Sciences Circular rna and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018237372A1 (en) * 2017-06-23 2018-12-27 Cornell University RNA MOLECULES, METHODS FOR PRODUCING CIRCULAR RNA, AND METHODS OF TREATMENT
WO2020219563A1 (en) * 2019-04-22 2020-10-29 TCR2 Therapeutics Inc. Compositions and methods for tcr reprogramming using fusion proteins
EP4116421A1 (en) * 2021-03-10 2023-01-11 Rznomics Inc. Self-circularized rna structure
WO2022247943A1 (en) * 2021-05-28 2022-12-01 Shanghai Circode Biomed Co., Ltd. Constructs and methods for preparing circular rnas and use thereof
WO2023046153A1 (en) * 2021-09-26 2023-03-30 Center For Excellence In Molecular Cell Science, Chinese Academy Of Sciences Circular rna and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ADAM ROTH ET AL.: "The structural and functional diversity of metabolite-binding riboswitches", ANNUAL REVIEW OF BIOCHEMISTRY, vol. 78, no. 1, 9 March 2009 (2009-03-09), US, pages 305 - 334, XP055566323, ISSN: 0066-4154, DOI: 10.1146/annurev.biochem.78.070507.135656 *
ALEXANDER SERGANOV ET AL.: "Ribozymes, riboswitches and beyond: regulation of gene expression without proteins", NATURE REVIEWS GENETICS, vol. 8, no. 10, 11 September 2007 (2007-09-11), pages 776 - 790, XP055107979, ISSN: 1471-0056, DOI: 10.1038/nrg2172 *

Also Published As

Publication number Publication date
WO2024236366A2 (en) 2024-11-21

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