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WO2024233705A3 - Recombinant host cell for producing aspartate - Google Patents

Recombinant host cell for producing aspartate Download PDF

Info

Publication number
WO2024233705A3
WO2024233705A3 PCT/US2024/028433 US2024028433W WO2024233705A3 WO 2024233705 A3 WO2024233705 A3 WO 2024233705A3 US 2024028433 W US2024028433 W US 2024028433W WO 2024233705 A3 WO2024233705 A3 WO 2024233705A3
Authority
WO
WIPO (PCT)
Prior art keywords
host cell
recombinant host
aspartic acid
producing
producing aspartate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/028433
Other languages
French (fr)
Other versions
WO2024233705A2 (en
Inventor
Nick OHLER
Rebecca Lennen
Azadeh ALIKHANI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lygos Inc
Original Assignee
Lygos Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lygos Inc filed Critical Lygos Inc
Publication of WO2024233705A2 publication Critical patent/WO2024233705A2/en
Publication of WO2024233705A3 publication Critical patent/WO2024233705A3/en
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Provided herein are host cells, such as recombinant microorganism host cells, for producing aspartic acid, preferably is amounts larger than produced by corresponding wild type cells, culturing them to produce aspartic acid or a salt thereof, and identifying cells that are thus suitable, and uses of aspartic acid produced thereby.
PCT/US2024/028433 2023-05-08 2024-05-08 Recombinant host cell for producing aspartate Pending WO2024233705A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202363464924P 2023-05-08 2023-05-08
US63/464,924 2023-05-08
US202363515294P 2023-07-24 2023-07-24
US63/515,294 2023-07-24

Publications (2)

Publication Number Publication Date
WO2024233705A2 WO2024233705A2 (en) 2024-11-14
WO2024233705A3 true WO2024233705A3 (en) 2025-04-03

Family

ID=93431139

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/028433 Pending WO2024233705A2 (en) 2023-05-08 2024-05-08 Recombinant host cell for producing aspartate

Country Status (1)

Country Link
WO (1) WO2024233705A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050019877A1 (en) * 2001-11-05 2005-01-27 Oskar Zelder Genes coding for metabolic pathway proteins
WO2007133184A2 (en) * 2005-04-26 2007-11-22 Cargill, Incorporated Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors
WO2010038905A1 (en) * 2008-09-30 2010-04-08 Ajinomoto Co.,Inc. A BACTERIUM BELONGING TO THE GENUS Pantoea PRODUCING AN L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES AND A METHOD FOR PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050019877A1 (en) * 2001-11-05 2005-01-27 Oskar Zelder Genes coding for metabolic pathway proteins
WO2007133184A2 (en) * 2005-04-26 2007-11-22 Cargill, Incorporated Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors
WO2010038905A1 (en) * 2008-09-30 2010-04-08 Ajinomoto Co.,Inc. A BACTERIUM BELONGING TO THE GENUS Pantoea PRODUCING AN L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES AND A METHOD FOR PRODUCING L-ASPARTIC ACID OR L-ASPARTIC ACID-DERIVED METABOLITES

Also Published As

Publication number Publication date
WO2024233705A2 (en) 2024-11-14

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