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WO2024233492A9 - Conception de cuvette destinée à être utilisée dans un module de cuvette - Google Patents

Conception de cuvette destinée à être utilisée dans un module de cuvette Download PDF

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Publication number
WO2024233492A9
WO2024233492A9 PCT/US2024/028033 US2024028033W WO2024233492A9 WO 2024233492 A9 WO2024233492 A9 WO 2024233492A9 US 2024028033 W US2024028033 W US 2024028033W WO 2024233492 A9 WO2024233492 A9 WO 2024233492A9
Authority
WO
WIPO (PCT)
Prior art keywords
cuvette
module
fluid
channel
region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/028033
Other languages
English (en)
Other versions
WO2024233492A1 (fr
Inventor
Robert GRANIER
Ramin Haghgooie
Daniel Hartmann
Jesse Newton JONES IV
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Fluidics Corp
Original Assignee
General Fluidics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Fluidics Corp filed Critical General Fluidics Corp
Priority to AU2024269943A priority Critical patent/AU2024269943A1/en
Publication of WO2024233492A1 publication Critical patent/WO2024233492A1/fr
Publication of WO2024233492A9 publication Critical patent/WO2024233492A9/fr
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/028—Modular arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0378—Shapes
    • G01N2021/0382—Frustoconical, tapered cell

Definitions

  • Cuvettes are small vessels, made from a transparent material, such as quartz, glass, or plastic. They are traditionally used for analysis of liquids with a spectrometer, fluorometer or spectrophotometer. They are also found in automated clinical chemistry and immunoassay analyzers where they are used to analyze biological fluids for determining patient health.
  • Flow-through cuvettes or flowcells also exist.
  • a flow-through cuvette has more than one opening, so that fluid may flow from a liquid inlet to a liquid outlet.
  • Such flow cells are used in instruments that include hematology analyzers, refractometers, particle counters, and tablet dissolution instruments, as well as certain fluorimeters, and spectrophotometers.
  • Flow cells are meant for in-line use; the input and output of the flow cell are joined to a fluidic network, and fluid is pumped into and out of the cell.
  • An advantage of these flow cells is that they are more-easily emptied and cleaned in-place.
  • a cleaning solution may be flowed through the flowcell directly to a waste reservoir to achieve the desired level of cleanliness.
  • traditional flowcells cannot be gravity-filled. Instead, a pressure gradient must be established to enable liquid to be pushed or pulled through the flowcell (e.g. through use of a pump). It is also difficult to mix two or more fluids together in a flowcell because the fluid is generally inaccessible to mechanical methods of mixing.
  • many flow cells have features such as expansions and contractions that make them prone to trapping bubbles during flow.
  • many flowcells are attached to the upstream fluidic network through unions which can leak.
  • aspects of the present disclosure include cuvettes, cuvette modules, and systems to conduct biological and chemical assays on a variety of samples.
  • One aspect of the present disclosure includes a cuvette for holding fluids, comprising: an inlet end comprising an inlet open to the atmosphere, wherein the inlet is configured to receive the fluids dispensed into the inlet from above the cuvette; a body of the cuvette connected to the inlet end, the body composed of walls surrounding the cuvette, each wall having an inner surface and an outer surface, the walls including a plurality of regions comprising: an inlet chamber, the inlet chamber comprising: an interrogation region comprising a proximal end and a distal end, the proximal end positioned at the inlet end of the cuvette, the walls at the interrogation region including a first wall and a second wall opposite the first wall, wherein the inlet chamber is configured to: be filled under the influence of gravity; and allow transmission of light through the first wall and the second wall of the interrogation region, and a tapered region comprising a proximal end and a distal end, the tapered region positioned at the distal end of
  • the necked region comprises an outlet channel.
  • the outlet channel comprises an outlet opening comprising an inner diameter ranging from 0.01 mm to 2 mm.
  • the outlet channel comprises an inner diameter ranging from 0.01 mm to 2 mm.
  • the necked region further comprises a restrictive channel.
  • the cuvette further comprising a capillary valve between the transition of the distal end of the tapered region and the proximal end of the restrictive channel.
  • the capillary valve is created by the transition of the inner diameter of distal end of the tapered region and the inner diameter of the proximal end of the restrictive channel.
  • the restrictive channel has an inner diameter ranging from 0.01 mm to 2 mm. In some embodiments, the restrictive channel is configured to hold a volume ranging from 0.01 microliters to 2 microliters.
  • the volume of the interrogation region of the cuvette is greater than 50% of the total volume of fluid that fills the cuvette
  • the restrictive channel comprises a length ranging from 0.5mm to 5 mm.
  • the inner diameter of the restrictive channel is smaller than the inner diameter of the outlet channel of the necked region.
  • the first and second walls of the interrogation region are substantially flat.
  • the obtuse angle between the inner surface of the wall of the tapered region and the inner surface of the first wall and the second wall of the interrogation region ranges from 130 to 179 degrees.
  • the cuvette comprises a connection point between the distal end of the interrogation region and the proximal end of the tapered region, wherein the connection point comprises the obtuse angle ranging from 130 to 179 degrees.
  • the cuvette further comprises an indexing feature configured to index a portion of the cuvette to a surface of a housing, a manifold, a sensor, or a fluidic valve.
  • the indexing feature is positioned at the inlet end.
  • indexing feature is positioned at the proximal end of the interrogation region.
  • the indexing feature comprises a peripheral rim surrounding the inlet.
  • the peripheral rim comprises a lip extending radially outward from the cuvette to lock the cuvette in place.
  • the indexing feature comprises a peripheral rim surrounding the inlet.
  • the indexing feature comprises a peripheral rim positioned between the distal end of the interrogation region and the proximal end of the tapered region.
  • the necked region is positioned at the distal end of the tapered region, and the outlet of the necked region is positioned at the distal end of the outlet channel.
  • the outlet of the cuvette has an opening with a diameter ranging from 0.1 mm to 2 mm.
  • the interrogation region of the cuvette comprises an inner dimension defining the optical path length that ranges from 1 mm to 10mm.
  • the cuvette is made of a transparent plastic chosen from: polycarbonate, polystyrene, acrylic, polypropylene, cyclic-olefm-copolymer, cyclic-olefm-polymer, copolyester, polyethylene terephthalate (PET), polyethylene terephthalate glycol (PETG), or other transparent plastic.
  • the cuvette is made of Eastman Tritan copolyester.
  • the cuvette, or portions thereof is made from a material that has a water/solid contact angle that is less than 90 degrees.
  • the cuvette is connected to a tube or channel at the distal end of the outlet.
  • the cuvette comprises means for changing the optical properties of light passing through the cuvette.
  • a cuvette module comprising: at least one compartment capable of holding at least one cuvette, the cuvette comprising an inlet open to the atmosphere, an inlet chamber, and an outlet, wherein inlet is configured to receive the fluids dispensed into the inlet from above the at least one cuvette; a central channel in fluidic communication with the outlet of the at least one cuvette; and a fluidic valve positioned between the central channel and a downstream channel and configured to connect and disconnect the downstream channel from the central channel.
  • the downstream channel is fluidically connected to and is downstream of the fluidic valve.
  • the cuvette module further comprises a fluidic seal between the outlet of the at least one cuvette and the central channel configured to create an air-tight seal between the outlet and the central channel.
  • the fluidic seal is a gasket positioned around the outlet end of the cuvette.
  • the outlet of the cuvette is configured to create a radial seal with the gasket.
  • the gasket is configured to provide an air-tight and/or fluid-tight seal between the outlet of the cuvette and a manifold of the cuvette module, the manifold comprising the central channel fluidically connecting the outlet of the cuvette and means for connecting to the fluidic valve.
  • the manifold comprises the downstream channel, the central channel, or the downstream channel and the central channel.
  • the gasket is positioned in the manifold. In some embodiments, the gasket is positioned around the outlet of the cuvette. In some embodiments, the fluidic seal is a radial O-ring seal or an axial gasket seal.
  • the fluidic valve when the fluidic valve is closed, the fluidic valve is configured to trap a volume of air between the fluidic valve and the inlet chamber of the at least one cuvette.
  • volume of air between the valve and any liquid in the cuvette inlet chamber ranges from 5 to 60 microliters.
  • the volume of air trapped between the fluidic valve and the inlet chamber of the cuvette is compressed if the fluid in the cuvette moves into the restrictive channel of the cuvette; wherein the compressed air generates a pressure that is equal and opposite to the head pressure of the fluid in the cuvette, thereby keeping the fluid in the inlet chamber of the cuvette.
  • the volume of air trapped between the fluidic valve and the inlet chamber of the cuvette is compressed if the fluid in the cuvette moves into the restrictive channel of the cuvette, wherein the compressed air then generates a pressure that, in combination with the capillary pressure associated with the capillary valve, is equal and opposite to the head pressure of the liquid in the cuvette, thereby keeping the fluid in the inlet chamber of the cuvette.
  • the cuvette comprises at least one cuvette.
  • the at least one cuvette comprises a body of the cuvette connected to the inlet end, the body composed of walls surrounding the cuvette, each wall having an inner surface and an outer surface, the walls including a plurality of regions comprising: the inlet chamber, the inlet chamber comprising: an interrogation region comprising a proximal end and a distal end, the proximal end positioned at the inlet end of the cuvette, the walls at the interrogation region including a first wall and a second wall opposite the first wall, wherein the inlet chamber is configured to: be filled, under the influence of gravity, with fluid dispensed into the inlet; and allow transmission of light through the first wall and the second wall of the interrogation region, and a tapered region comprising a proximal end and a distal end, the tapered region positioned at the distal end of the interrogation region, the inner surface of the wall at the tapered region tapering inward from the proximal end of the tapered region to
  • the fluidic valve when the fluidic valve is closed, the fluidic valve is configured to trap a volume of air between the fluidic valve and the necked region of the cuvette. In some embodiments, the open position of the fluidic valve creates a fluidic connection between the outlet of the cuvette and the downstream channel. [21] In some embodiments, the volume or air between the fluidic valve and the inlet chamber ranges from 5 and 60 microliters. In some embodiments, wherein the module further comprises mechanical means of holding the cuvette in a fixed position, wherein the inlet is maintained in an orientation and position for fluid to be dropped or dispensed into the inlet of the cuvette, thereby filling the inlet chamber of the cuvette by means of gravity.
  • the cuvette module further comprises: a housing having one or more housing walls configured to hold the at least one cuvette in the fixed position, and an opening at the proximal end of the housing walls for insertion of the cuvette into the housing.
  • the module further comprises a cap configured to attach to the housing over the inlet of the at least one cuvette.
  • the housing is made of a thermally conductive material.
  • the thermally conductive material is aluminum.
  • the housing comprises a slot on at least one of the housing walls to register or index the at least one cuvette in a selected position within the housing.
  • the housing comprises a fastener on at least one of the housing walls to fasten the cuvette into the housing.
  • the at least one cuvette comprises two or more cuvettes, each cuvette positioned in a separate opening or slot of the housing for inserting and indexing each cuvette.
  • the cuvette module comprises two or more fluidic valves.
  • the cuvette module comprises two or more downstream channels within a manifold.
  • the cuvette module comprises two or more central channels within a manifold.
  • each fluidic valve is configured to fluidically connect to each central channel, and wherein each central channel is configured to be in fluid communication with the outlet of each cuvette.
  • the module further comprises means of directing and detecting light that has interacted with at least a portion of the fluid contained in the at least one cuvette. In some embodiments, the module further comprises means of directing light from a light source so that it impinges on and passes through a first wall of the cuvette and interacts with at least a portion of the liquid contained therein.
  • the cuvette module further comprises:a light source positioned adjacent to the interrogation region of the cuvette, the light source configured to deliver a beam of light through the interrogation region of the cuvette; and an optical detector disposed in optical communication with the interrogation region of the cuvette to receive at least a portion of the beam that has traversed the cuvette.
  • the cuvette module comprises two or more light sources and two or more optical detectors, each light source positioned adjacent to the interrogation region of each cuvette, and each optical detector positioned adjacent to the interrogation region of each cuvette.
  • the module further comprises means of filtering light passing through the cuvette to change the wavelength content, polarization, and/or intensity of the light.
  • the module further comprises a filter housing configured to hold one or more optical filters, each optical filter positioned adjacent to each cuvette. In some embodiments, the module further comprises an optical mask.
  • the cuvette module is configured to enable light from a light source to enter the cuvette through a first substantially flat wall of the cuvette, interact with at least a portion of the fluid contained within the at least one cuvette, and exit through a second substantially flat wall of the at least one cuvette, wherein the remaining light is collected by a detector.
  • the module further comprises a heating and/or cooling element configured to control the temperature of the cuvette and the fluid within the cuvette.
  • the optical detector is a silicon photodiode.
  • the module comprises a sensor configured to detect the presence or absence of fluid in at least a portion of the cuvette.
  • the module comprises a pressure sensor.
  • the module comprises a tube or channel directed connected to an input of the cuvette, wherein the tube or channel is configured to hold and move a solution into the input of cuvette to fill the cuvette inlet chamber.
  • the module comprises one or more channels fluidically downstream of one or more fluidic valves, wherein the one or more fluidic channels is connected to the distal end of one or more fluidic valves.
  • the module comprises a pump connected to one or more channels that are downstream of and fluidically connected to, the outlet of the cuvette.
  • the module comprises the pump is configured to trap a pocket or volume of air between the pump and any fluid in the inlet chamber of the cuvette.
  • the module comprises the pump is selected from: a peristaltic pump, a membrane pump, or a diaphragm pump.
  • the module comprises the at least one cuvette is the at least one cuvette of any one of claims 1-31.
  • Another aspect of the present disclosure includes a module having a plurality of cuvettes, fluidic valves, central channels, and downstream channels of the present disclosure.
  • the fluidic channels downstream of the one or more fluidic valves are joined to a common or adjoining channel.
  • Another aspect of the present disclosure includes a method of using the module of the present disclosure to perform a photometric assay, the method comprising: (a) mechanically constraining the cuvette in the module in a fixed location and orientation; (b) closing the fluidic valve in the module; (c) dropping or dispensing fluid into the inlet of the cuvette so that it fills the inlet chamber of the cuvette, wherein a volume or pocket of air is trapped between the fluidic valve and the fluid in the inlet chamber of the cuvette; (d) directing light from a light source into the cuvette, wherein the light is configured to interact with at least a portion of the fluid contained within the inlet chamber of the cuvette; (e) collecting light that has interacted with the fluid with a light-detector and gathering data representative of changes occurring in the fluid; and (f) opening the fluidic valve of the module and pulling or pushing, with a pump, the fluid within the cuvette through the outlet of the cuvette and into the downstream channel.
  • the method further comprises, after step c), optionally processing the fluid of the cuvette by performing one or more steps selected from: adding additional fluids or solids to the cuvette; mixing the fluids or solids within the cuvette; and heating or cooling the mixed fluids or solids within the cuvette.
  • the method further comprises, after step e), analyzing the data.
  • the method comprises, after step f), cleaning the cuvette by: closing the fluidic valve; filling the cuvette with a solution; opening the fluidic valve; and pulling or pushing the cleaning fluid through the outlet of the cuvette and into a downstream channel.
  • the method further comprises measuring a polarization change of the light interacting with the fluid within the cuvette. In some embodiments, the method further comprises measuring a fluorescence emission when the light interacts with the fluid within the cuvette.
  • Another aspect of the present disclosure includes a fluidic system comprising the cuvette module of the present disclosure and a downstream pump.
  • the pump is attached to a tube or channel that is connected to the fluidic valve, wherein when the fluidic valve is closed, the pump is fluidically disconnected from the cuvette, and wherein when the valve is open, the pump is fluidically connected to the cuvette.
  • the system further comprises a waste tank.
  • the system further comprises a cleaning fluid tank.
  • the fluidic system further comprises a cleaning pump.
  • the fluidic system further comprises a degasser.
  • the fluidic system further comprises one or more pipettes.
  • the fluidic system comprises the at least one cuvette of the present disclosure.
  • the fluidic system further comprises a mixing apparatus.
  • Another aspect of the present disclosure includes a method of using the cuvette module of the present disclosure to perform a photometric assay, the method comprising: (a) mechanically constraining a cuvette in the module in a fixed location and orientation; (b) dropping or dispensing fluid into the inlet of the cuvette so that it fills the inlet chamber of the cuvette, wherein a volume or a pocket of air is trapped between the pump and the fluid in the inlet chamber of the cuvette; (c) directing light from a light source into the cuvette, wherein the light is configured to interact with at least a portion of the fluid contained within the inlet chamber of the cuvette; (d) collecting light that has interacted with the fluid with a light-detector and gathering data representative of changes occurring in the fluid; and (e) pulling, using the pump, the fluid within the cuvette through the outlet of the cuvette to the downstream channel.
  • the method further comprises, after step b), using the pump to slightly-increase the pressure in the volume or pocket of air between the cuvette and the pump. In some embodiments, method further comprises, after using the pump to slightly-increase the pressure in the volume or pocket of air between the cuvette and the pump, the method further comprises processing the fluid of the cuvette by performing one or more steps selected from: adding additional fluids or solids to the cuvette; mixing the fluids or solids within the cuvette; and heating or cooling the mixed fluids or solids within the cuvette.
  • the method further comprises, after step d), the method further comprises analyzing the data. In some embodiments, the method comprises, after step e), cleaning the cuvette by:filling the cuvette with a wash solution; and pulling the cleaning fluid through the outlet of the cuvette and into a downstream channel
  • a cuvette module comprising: at least one compartment capable of holding at least one cuvette, the cuvette comprising an inlet open to the atmosphere, an inlet chamber, and an outlet, wherein inlet is configured to receive the fluids dispensed into the inlet from above the at least one cuvette; a central channel in fluidic communication with the outlet of the at least one cuvette; and a pump connected between the central channel and a downstream channel, wherein the downstream channel is positioned downstream of the pump.
  • the pump is configured to trap a pocket or volume of air between the pump and any fluid in the inlet chamber of the cuvette.
  • the pump is selected from: a peristaltic pump, a membrane pump, or a diaphragm pump.
  • Another aspect of the present disclosure includes a cuvette comprising an inlet end comprising an inlet open to the atmosphere, wherein the inlet is configured to receive the fluids dispensed into the inlet from above the cuvette; a body of the cuvette connected to the inlet end, the body composed of walls surrounding the cuvette, each wall having an inner surface and an outer surface, the walls including a plurality of regions comprising: an inlet chamber, the inlet chamber comprising: an interrogation region comprising a proximal end and a distal end, the proximal end positioned at the inlet end of the cuvette, the walls at the interrogation region including a first wall and a second wall opposite the first wall, wherein the inlet chamber is configured to: be filled under the influence of gravity; and allow transmission of light through the first wall and the second wall of the interrogation region, and a tapered region comprising a proximal end and a distal end, the tapered region positioned at the distal end of the interrogation region,
  • Another aspect of the present disclosure include a cuvette that has: an interrogation region that can be loaded and held under the influence of gravity through an input, but emptied from a separate output; a design to ensure that the cuvette may be filled with minimal chance of bubble entrainment occurring in the interrogation region, and a design that maintains the liquid within the interrogation region for as long as desired, and then be easily emptied to remove all, or almost all the liquid within it.
  • Another aspect of the present disclosure includes cuvette geometries that minimize the total fluid volume required to fill them, while maintaining the volume of fluid that interacts with light during photometric assays.
  • Another aspect of the present disclosure includes unique cuvette modules into which at least one cuvette may be removably-inserted; the modules enabling the cuvette to be easily integrated with all the hardware required to perform a desired assay.
  • Another aspect of the present disclosure includes cuvette designs that provide an indexing feature configured to index a portion of the cuvette to a surface of a housing, a manifold, a sensor, or a fluidic valve.
  • the indexing feature is to enable it to be removably-inserted into a module in a precise position.
  • Another aspect of the present disclosure includes a system and methods that use the cuvettes and cuvette modules to conduct biological and chemical assays on a variety of samples.
  • One aspect of the present disclosure includes a cuvette for holding fluids, comprising: an inlet end comprising an inlet open to the atmosphere, wherein the inlet is configured to receive the fluid that is dropped or dispensed into the inlet from above the cuvette.
  • the cuvette further comprises a body of the cuvette connected to the inlet end, the body composed of walls surrounding the cuvette, each wall having an inner surface and an outer surface, the walls including a plurality of regions comprising: an inlet chamber containing an interrogation region comprising a proximal end and a distal end, the proximal end positioned at the inlet end of the cuvette, the walls at the interrogation region including a first wall and a second wall opposite the first wall, wherein the inlet chamber is configured to: be filled, under the influence of gravity, by the fluid that is dropped or dispensed into the inlet; and allow transmission of light through the first wall and the second wall of the interrogation region, and a tapered region comprising a proximal end and a distal end, the tapered region positioned at the distal end of the interrogation region, the inner surface of the wall at the tapered region tapering inward from the proximal end of the tapered region to a narrowed section at the distal end
  • the first and second walls of the interrogation region are substantially flat.
  • the obtuse angle between the inner surface of the wall of the tapered region and the inner surface of the first wall and the second wall of the interrogation region ranges from 130 to 179 degrees.
  • the cuvette comprises a connection point between the distal end of the interrogation region and the proximal end of the tapered region, wherein the connection point comprises the obtuse angle ranging from 130 to 179 degrees.
  • the cuvette comprises an indexing feature.
  • the indexing feature is configured to index a portion of the cuvette to a surface of a housing, a manifold, a sensor, or a fluidic valve of a cuvette module of the present disclosure.
  • the inlet end further comprises the indexing feature.
  • the indexing feature is positioned at the proximal end of the interrogation region.
  • the indexing feature comprises a peripheral rim surrounding the inlet.
  • the peripheral rim comprises a lip extending radially outward from the container to lock the container in place.
  • the indexing feature is connected to the first and second walls. In some embodiments, the indexing feature comprises a peripheral rim surrounding the inlet. In some embodiments, the cuvette further comprises an indexing feature, wherein the indexing feature comprises a peripheral rim positioned between the distal end of the interrogation region and the proximal end of the tapered region.
  • the outlet is positioned at the distal end of the tapered region.
  • the body of the cuvette further comprises a necked region positioned at the distal end of the tapered region and comprises an outlet at the distal end of the necked region.
  • the outlet of the cuvette has an opening that is between 0.1 mm and 2 mm in diameter.
  • the necked region comprises an outlet channel that fluidically joins the distal end of the tapered region with the outlet.
  • the outlet channel is restrictive, having an inner diameter that is between 0.1 and 1mm in diameter.
  • the necked region comprises two channels: a restrictive channel and an outlet channel, wherein the restrictive channel is proximal to and in fluid communication with the distal end of the tapered region, the distal end of the restrictive channel is connected to the proximal end of the outlet channel, and the distal end of the outlet channel is connected to the outlet.
  • the restrictive channel and the outlet channel are in series and fluidically connect the distal end of the tapered region to the outlet.
  • the restrictive channel is between 0.1 and 1mm in diameter, and the outlet channel is between 0.3mm and 2mm in diameter.
  • the outlet of the cuvette is fluidically-restricted through use of a restrictive element inserted into a necked region of the cuvette, the restrictive element having an opening that is between 0.1 mm and 1 mm in diameter.
  • the cuvette is made of a transparent plastic chosen from: polycarbonate, polystyrene, acrylic, polypropylene, cyclic-olefm-copolymer, cyclic-olefm-polymer, copolyester, polyethylene terephthalate (PET), polyethylene terephthalate glycol (PETG), or other transparent plastic.
  • a transparent plastic chosen from: polycarbonate, polystyrene, acrylic, polypropylene, cyclic-olefm-copolymer, cyclic-olefm-polymer, copolyester, polyethylene terephthalate (PET), polyethylene terephthalate glycol (PETG), or other transparent plastic.
  • the cuvette is made of Eastman Tritan copolyester.
  • the cuvette is connected to a tube or channel at the distal end of the outlet.
  • a cuvette for holding fluids comprising: an inlet end comprising an inlet open to the atmosphere, wherein the inlet is configured to receive the fluid dropped or dispensed into the inlet from above the cuvette; a body of the cuvette connected to the inlet end, the body composed of walls surrounding the cuvette, each wall having an inner surface and an outer surface, the walls including a plurality of regions comprising: an inlet chamber containing an interrogation region comprising a proximal end and a distal end, the proximal end positioned at the inlet end of the cuvette, the walls at the interrogation region including a first wall and a second wall opposite the first wall, the interrogation region configured to: be filled, under the influence of gravity, by the fluid dispensed into the inlet; and allow transmission of light through the first wall and the second wall of the interrogation region; and an outlet end fluidically connected to the body of the cuvette, the outlet end comprising an outlet that behaves as a
  • the cuvette further comprises a pinching element for creating a tapered region comprising a proximal end and a distal end, the tapered region positioned at the distal end of the interrogation region, the inner surface of the wall at the tapered region tapering inward from the proximal end of the tapered region to a narrowed section at the distal end of the tapered region, wherein there is an obtuse angle formed between the inner surface of the wall at the tapered region and the inner surface of the first wall and the second wall at the interrogation region to enable drainage of the fluid from the cuvette once the fluid is measured.
  • the pinching element is configured to close or open the outlet of the cuvette.
  • the pinching element is configured to reversibly create a necked region of the cuvette and the tapered region.
  • the cuvette is connected to a tube or channel at the distal end of the outlet.
  • the cuvette has a trapezoidal cross-section.
  • the cuvette is coated with or is made from material that alters the characteristics of light passing through the cuvette in a way that enables or improves the performance of an assay performed therein.
  • a cuvette module comprising: a cuvette design of the present disclosure; a housing comprising one or more housing walls shaped to hold and mechanically constrain the cuvette in place.
  • the housing comprises an opening at a proximal end of the housing walls for inserting the cuvette into the housing.
  • the housing comprises a slot on at least one of the housing walls to register or index the cuvette in a selected position within the housing.
  • the housing comprises a fastener on at least one of the housing walls to fasten the cuvette into the housing.
  • the cuvette module further comprises a manifold having a fluidic channel positioned between and fluidically connecting the outlet of the cuvette and a fluidic valve.
  • the manifold further comprises a gasket positioned around the outlet end of the cuvette.
  • the outlet end of the cuvette is configured to create a radial seal with the gasket.
  • the gasket is configured to provide an air-tight and/or fluid-tight seal between the manifold and the outlet of the cuvette.
  • the gasket is configured to provide an air-tight and/or fluid-tight seal between the first fluidic channel and the outlet of the cuvette.
  • the cuvette module further comprises an active fluidic valve (e.g., a valve that is controlled by an externally-applied signal), fluidically connected to the central channel of the manifold, wherein the active valve may open and close, such that opening the valve creates a fluid path from the outlet of the cuvette to a channel downstream (e.g., downstream channel) of the valve, and closing the valve disconnects the outlet of the cuvette from the downstream channel. If there is liquid in the cuvette, closing the valve also traps a small volume of air between the liquid and the valve.
  • an active fluidic valve e.g., a valve that is controlled by an externally-applied signal
  • the cuvette module further comprises a second gasket that is configured to provide an air-tight and/or fluid-tight seal between the active fluidic valve and the manifold.
  • the cuvette module further comprises: a light source positioned adjacent to the interrogation region of the container, the light source configured to deliver a beam of light through the interrogation region of the cuvette; and an optical detector disposed in optical communication with the interrogation region of the cuvette to receive at least a portion of the beam that has traversed the cuvette.
  • the cuvette module further comprises openings into which optical elements, such as filters and lenses may be removably inserted, such that light passes through the optical elements before and/or after passing through the interrogation region of the cuvette.
  • the cuvette is one of an array of cuvettes, each of the cuvettes positioned within a housing. In some embodiments, each cuvette within the array of cuvettes is positioned in a separate opening or slot for inserting and indexing each cuvette.
  • the cuvette module further comprises an array of active fluidic valves.
  • the cuvette module further comprises a manifold comprising an array of fluidic channels, each fluidic channel positioned between and fluidically connecting each outlet of each of the cuvettes and each active fluidic valve.
  • each active valve is fluidically connected to the fluidic channel of the manifold, wherein each active valve is configured to open and close, such that opening the valve creates a fluid path from the outlet of the cuvette to a channel downstream of the valve, and closing the valve disconnects the outlet of the cuvette from the downstream channel. If there is liquid in the cuvette this action also traps a small volume of air between the closed valve and the liquid.
  • the volume of air trapped between liquid in the cuvette and the active valve is small, such that the head pressure of the liquid in the cuvette is substantially smaller than the increase in the air pressure that would result from the compression of air if fluid was to pass through the restrictive element in the necked region of the cuvette.
  • the active valve acts in association with the restrictive region (e.g., restrictive channel) in the necked region of the cuvette to maintain liquid in the inlet chamber of the cuvette until the active valve is opened.
  • restrictive region e.g., restrictive channel
  • the small volume of air trapped between the cuvette and the closed active valve becomes pressurized above atmospheric pressure if liquid moves into the restrictive channel in the necked region of the cuvette.
  • the array of cuvettes comprises 2 or more cuvettes.
  • the array of active fluidic valves comprises 2 or more fluidic active valves.
  • the cuvette module further comprises an array of light sources and an array of optical detectors, each light source positioned adjacent to the interrogation region of each cuvette, and each optical detector positioned adjacent to the interrogation region of each cuvette.
  • the cuvette module further comprises an array of cuvettes.
  • the cuvette module further comprises a tube or channel connected to the distal end of the cuvettes.
  • Another aspect of the present disclosure includes a system for performing photometric measurements of a fluid, the system comprising the cuvette module of the present disclosure, and a pump.
  • the system further comprises a waste tank. In some embodiments, the system further comprises a cleaning fluid tank. In some embodiments, the microfluidic system further comprises a cleaning pump. In some embodiments, the microfluidic system further comprises a degasser. In some embodiments, the microfluidic system further comprises one or more pipettes. In some embodiments, the one or more pipettes comprise one or more automated pipettes. In some embodiments, the system further comprises a thermal heater.
  • Another aspect of the present disclosure includes methods for processing and performing a photometric measurement.
  • the method comprises : (a) dispensing a fluid, under the influence of gravity, into an inlet of the cuvette design of the present disclosure from above the cuvette to deliver the fluid into the cuvette; (b) processing the fluid while holding it within the inlet chamber.
  • the method further comprises (c) transmitting light from a first light source to a photodetector through the interrogation region of the cuvette containing the fluid; (d) acquiring data representing the fluid; (e) determining the presence or absence of one or more analytes in the fluid.
  • the method further comprises, step (f) draining the fluid from the cuvette through the outlet and washing the cuvette to prepare it to process more fluid.
  • the method further comprises, before step (e), analyzing the data representing the fluid.
  • analyzing comprises determining a concentration of the one or more analytes in the fluid.
  • acquiring data representing said fluid comprises acquiring fluorometric measurements of the fluid.
  • acquiring data representing the fluid comprises measuring the absorbance of the fluid. In some embodiments, acquiring data representing the fluid comprises measuring the concentration of the fluid.
  • the one or more analytes are selected from: Blood urea nitrogen (BUN), carbon dioxide (CO2), Creatinine, Glucose, chloride, potassium, sodium, calcium, Hemoglobin, Albumin (ALB), Total protein (TP), Alkaline phosphatase (ALP), Alanine transaminase (ALT), Aspartate aminotransferase (AST), Total Bilirubin, Amylase, Gamma-glutamyl transferase, Lipase, Magnesium, Phosphorus, Direct Bilirubin, Triglycerides, total Cholesterol, high-density- lipoprotein (HDL), Ammonia, lactic acid (LAC), Fructose, Lactate dehydrogenase (LDH), uric acid, bile acids, Hbalc, and Creatine kinase.
  • BUN Blood urea nitrogen
  • CO2 carbon dioxide
  • Creatinine Glucose
  • chloride potassium
  • sodium
  • analyzing comprises determining a mean corpuscular hemoglobin concentration (MCHC) in the fluid. In some embodiments, analyzing comprises determining a mean corpuscular hemoglobin (MCH) in the fluid.
  • MCHC mean corpuscular hemoglobin
  • FIG. 1 shows a conventional spectrophotometer and chemistry analyzer and conventional cuvettes for use regarding the same.
  • FIG. 2A-2D shows an exemplary cuvette design of the present disclosure comprising an inlet, an outlet, and a body of a cuvette positioned between the inlet and outlet.
  • the body includes an inlet chamber (206) that contains an interrogation region.
  • the body also contains a tapered region and a necked region.
  • the necked region contains an output channel and a restrictive channel that is proximal to the inlet chamber.
  • the restrictive channel is small enough in diameter and/or has a low- enough surface energy that a liquid/air interface formed at the restricted region creates an upward force greater than or equal to the force of gravity pushing the liquid down, so that liquid stays in the inlet chamber and does not leave the cuvette.
  • FIGs. 2B-2C show examples of cuvettes with necked regions having restrictive channels.
  • the outlet channel (240) and the restrictive channel (210) are one-and-the same, and the channel spans the full- length of the necked region;
  • the restrictive channel (210) is at the top of the necked region, proximal to the tapered region, and is in series with a larger-diameter outlet channel (240).
  • FIGs 2D-2E show examples of cuvettes in which (FIG. 2D) the tapered region makes a gradual and smooth transition to the restrictive channel of the necked region: (FIG. 2E) the tapered region transitions to a flat section, and there is an abrupt transition to the restrictive channel of the necked region.
  • FIG. 3 shows a cross-sectional view of an exemplary cuvette design of the present disclosure positioned so that it is proximal to a light-emitter and a detector. A cone of light from the emitter is also illustrated.
  • FIGs. 4A-4B provides isometric and cross-sectional views of exemplary cuvette design of the present disclosure, where the cuvette design can be injection moldable.
  • the cuvette can include a peripheral rim surrounding the inlet end of the cuvette, and a connection point comprising an obtuse angle between the distal end of the interrogation region and the proximal end of the tapered region of the cuvette.
  • FIG. 4B provides isometric and cross-sectional views of a second exemplary cuvette design of the present disclosure.
  • the cuvette can include a peripheral rim surrounding the cuvette, and positioned at the proximal end of the tapered region.
  • FIG. 5A-5D show a cross-sectional view of an exemplary cuvette design of the present disclosure that is fluidically connected to a downstream active valve.
  • FIG 5B illustrates the forces on the liquid when the liquid has filled the cuvette up to the opening of the restrictive channel in the necked region.
  • FIG 5C shows the forces on the liquid when the liquid has entered the restrictive channel in the necked region.
  • FIGs. 5D show the forces acting on the liquid when the liquid has filled the restrictive channel.
  • FIG. 6 shows an example of a cuvette having a necked region with a relatively large inner diameter, into which a restrictive element has been press-fit.
  • FIGs. 9A-9B provide a non-limiting isometric views of a cuvette according to one embodiment, where the cuvette is dyed (FIG. 9A) or coated (FIG. 9B) with one or more colors or coatings to alter the properties of light transmitted through the cuvette.
  • Such alterations include altering the spectrum, polarity, or intensity of light transmitted through the cuvette.
  • FIGs. 11A-11D show examples in which a plurality of cuvettes are joined together, for example, either through a monolithic fabrication process, or by assembly of discrete cuvettes into a whole.
  • the plurality of cuvettes can be in the form of ID and 2D arrays, or any other desirable configuration.
  • FIG. 11 A shows representative schematic of a ID array of cuvettes.
  • FIG. 1 IB shows an array of molded cuvettes assembled into a circular holder.
  • FIG. 11C shows an isometric and cross-sectional view of a monolithic array of cuvettes in a ID array.
  • FIG. 1 ID shows a two-dimensional array of molded cuvettes.
  • FIG. 13 shows the components of an exemplary multiplexed cuvette module including an array of cuvettes, and a single-unit-cell of the cuvette module.
  • FIG. 14 shows an exploded view of the exemplary multiplexed cuvette module of the present disclosure of FIG. 17.
  • FIGs. 15A-15K show cross-sectional views of an exemplary cuvette module of the present disclosure comprising a cuvette, a housing, a fluidic manifold, and a fluidic valve or pump.
  • the exemplary cuvette module shown in FIG. 15A provides an example of a radial Ciring seal configured to provide an air-tight and/or fluid tight seal between of the cuvette and the manifold.
  • the exemplary cuvette module shown in FIG. 15B provides an example of an axial gasket seal configured to provide an air-tight and/or fluid tight seal between the cuvette and the manifold.
  • FIG. 15C illustrates the state of the module before liquid has been dropped or dispensed into the cuvette. The cuvette and the tube are at atmospheric pressure.
  • FIG. 15D illustrates the state of the module when liquid has been dropped or dispensed into the cuvette, thereby trapping a volume of air, VI, between the valve and the liquid.
  • FIG. 15E illustrates the state of the module wherein some of the liquid in the cuvette begins to pass into the restrictive channel of the cuvette, thereby compressing the trapped volume of air to a volume V2.
  • FIG. 15F illustrates a state in which the cuvette is being emptied; the valve is opened and a downstream pump (not shown) pulls the fluid out of the cuvette.
  • FIG. 15G illustrates the state of the module before liquid has been dropped or dispensed into the cuvette.
  • the cuvette, tube and upstream side of the pump are all at atmospheric pressure.
  • FIG. 15H illustrates an embodiment in which liquid has been dropped or dispensed into the cuvette, thereby trapping a volume of air, VI, between the pump and the liquid in the inlet chamber of the cuvette.
  • FIG. 151 illustrates an alternative situation wherein some of the liquid in the cuvette begins to pass into the restrictive channel of the cuvette, thereby compressing the trapped volume of air to a volume V2.
  • FIG. 15J illustrates an optional aspect of this embodiment in which the pump may be configured to apply a very slight pressure to the air-chamber, thereby momentarily increasing the pressure in the trapped air volume above the head-pressure of the liquid.
  • FIG. 15K illustrates a state in which the cuvette is being emptied by the pump which pulls the fluid out of the cuvette.
  • FIGs. 16A-16B show non-limiting examples of a cuvette module of the present disclosure.
  • FIG. 16A shows a cross-sectional view of an exemplary cuvette module of the present disclosure comprising a cuvette, a housing, a fluidic manifold, and a fluidic valve.
  • FIG. 16B shows an isometric view of a cuvette module that includes one or more filter holders within the housing that can accept optical filters.
  • FIG. 17 shows cross-sectional views of an exemplary cuvette module of the present disclosure comprising a cuvette, and a soft rubber tube positioned at the end of the outlet of the cuvette that serves the purpose of a manifold.
  • the cuvette module in this exemplary embodiment includes a pinching element that prevents fluid from draining through the outlet when the pinching element is closed, but permits fluid to be drained when the pinching element is open.
  • FIG. 18 shows an exemplary fluidic system design comprising a cuvette of the present disclosure, cuvette module and a fluidic base with channels or tubes connecting individual cuvettes to a single waste chamber.
  • FIG. 19A-19B show a workflow schematic of the methods of the present disclosure using a cuvette module to fill and mix liquids in a cuvette of the present disclosure, measure and analyze the sample, and empty the cuvette after the sample has been measured and analyzed.
  • FIG. 20 shows the fluid architecture of an exemplary system of the present disclosure.
  • FIG. 21 shows a process flow of an exemplary method of the present disclosure (e.g., clinical chemistry) using the cuvette module cuvette module of the present disclosure.
  • FIG. 22 shows a non-limiting example of a fluidic system of the cuvette module comprising a tube or channel connected to a hole within the cuvette for dispensing fluids into the cuvette.
  • FIGs. 23A-23B show alternate cuvette embodiments wherein FIG 21 A is representative of the cuvettes we have described thus far wherein the interrogation region is contained within the inlet chamber, and FIG 2 IB are designs in which the interrogation region is separate from the inlet chamber.
  • FIG. 24 shows a cuvette module system containing the integrated module, a wash station, and a pipette system for use in processing samples.
  • FIG. 25 shows a cuvette module that includes a circular array of cuvettes. 5. DETAILED DESCRIPTION
  • sample refers to a sample of fluid material that is assumed to contain an analyte of interest.
  • samples include bodily fluids (such as whole blood, serum, plasma, cerebrospinal fluid, urine, lymph fluids), and other fluids (such as, for example, cell culture suspensions, cell extracts, cell culture supernatants).
  • a sample may be suspended or dissolved in, for example, buffers, extractants, solvents, and the like. Additional examples of samples are provided by fluids deliberately created for the study of biological processes or discovery or screening of drug candidates. The latter include, but are not limited to, aqueous samples containing bacteria, viruses, DNA, polypeptides, natural or recombinant proteins, metal ions, or drug candidates and their mixtures.
  • aspects of the present disclosure include a container for holding a fluid, such as a cuvette or tube or channel, or other fluid holder. It can be used in a device or instrument for performing measurements or testing.
  • the container or cuvette is used in a cuvette module for performing a photometric measurement.
  • the term “cuvette” is used throughout for illustration, but it is understood that this can be any type of sample holder or container, such as a test tube, a well or microwell in a plate, centrifuge tube, etc.
  • the present disclosure provides cuvette designs that can be used in an instrument capable of performing a plurality of clinical chemistry, hematology, and/or immunoassays. Preferably, it is a multi-modal instrument capable of running assays of all these types.
  • the cuvettes of the present disclosure are designed to be used in photometric and radiometric assays (the words “photometry,” and “photometric” are used interchangeably herein). These assays involve the detection of light intensity as a means of quantifying an analyte. This broadly includes absorption, scattering, polarization, fluorescence, and luminescence-based assays, which have been widely adopted as tools for determining concentrations of analytes in both human and animal biological samples such as, for example, blood, urine, and saliva, to name just a few. In vitro diagnostic devices using photometric detection techniques have been developed for a large variety of clinical biomarkers.
  • An aspect of the present disclosure includes a cuvette for use in photometric measurements.
  • an example of the cuvette of the present disclosure includes cuvette 200 comprising an inlet 201, an outlet 202, and a body 203.
  • the design of the cuvette allows for prevention of trapped air bubbles inside of the cuvette; complete drainage of the cuvette after measurement of the sample leaving behind no residual fluid; elimination of sharp edges or comers where fluid may pool; maintaining fluid within the interrogation region for as long as desired, but to exit the cuvette upon application of an additional pressure drop across the outlet, and provides an optical path length for a light beam traversing its width that is sufficient for the desired photometric assay(s).
  • the design and orientation of the cuvette is such that gravity ensures that fluids provided through the inlet enter and uniformly fill the inlet chamber (206) and the interrogation region (207).
  • the cuvette also includes a restrictive channel (210) located in the necked region (209) of the cuvette. This restriction helps maintain all the liquid in the inlet chamber while the liquid is being processed.
  • the cuvette design also allows for processing of the sample and measurement of a sample in a single cuvette design.
  • the cuvette of the present disclosure allows pipetting and active mixing of reagents and the sample inside a single cuvette prior to or during measurement of the sample.
  • the cuvette is designed so that when it is placed into a corresponding cuvette module, and loaded with the expected fluid volume (e.g. sample, reagents, or sample + reagents), the liquid volume fills the inlet chamber comprising the interrogation region (207) that is proximal to the position of a light emitter and a detector.
  • the expected fluid volume e.g. sample, reagents, or sample + reagents
  • the liquid volume fills the inlet chamber comprising the interrogation region (207) that is proximal to the position of a light emitter and a detector.
  • the emitter and detector are positioned such that they are approximately halfway between the cuvette taper and the top of the liquid volume, so that the cone of light from the emitter is not disturbed by the liquid/air meniscus, or the taper of the cuvette.
  • the cuvette of the present disclosure is designed such that the sample can be processed and optically measured at the same time. Alternatively, the sample can also be processed first within the cuvette, followed by measuring the sample.
  • a feature of this cuvette is that the interrogation region of the inlet chamber of the cuvette does not require a pressure gradient to fill it since it relies on gravity to allow fluid, once received by the inlet of the cuvette, to fill the inlet chamber, which contains the interrogation region.
  • the capillary pressure created by a liquid/air interface at the outlet of the necked region of the cuvette is greater than the force of gravity acting on the liquid, thereby preventing the liquid from leaving the cuvette.
  • the cuvette includes a restrictive channel positioned between the tapered region of the cuvette and the outlet channel of the necked region.
  • the capillary pressure created by a liquid/air interface at this restrictive fluidic channel is greater than the force of gravity acting on the liquid, thereby preventing the liquid from leaving the cuvette for as long as desired during processing.
  • the cuvette can be emptied by applying a pressure drop across the fluid (e.g. with a pump).
  • the cuvette includes an inlet end comprising an inlet (201) open to the atmosphere.
  • the inlet is configured to receive a sample dropped or dispensed into the inlet from above the cuvette, wherein the fluid enters the inlet chamber under the influence of gravity.
  • the cuvette can be positioned within a cuvette module such that fluids can be pipetted into the inlet and the interrogation region of an inlet chamber will fill the interrogation region under the influence of gravity.
  • the fluid can be dropped, e.g., via gravity, into the inlet, from above the cuvette.
  • the fluid can be dispensed, e.g., through a pipettor, into the inlet, from above the cuvette.
  • the inlet chamber comprising an interrogation region fills with fluid under the influence of gravity to a level determined only by the force of gravity.
  • the fluid equilibrates at the same level in the inlet chamber under influence of gravity.
  • the size and orientation of the inlet chamber and interrogation region are such that the force of gravity within them is larger than any other forces within them (such as surface tension and capillary forces); therefore liquid uniformly fills these volumes without entrapment of air bubbles.
  • the inlet end further comprises an indexing feature.
  • the indexing feature is positioned at the proximal end of the interrogation region (e.g., closest to the inlet).
  • the indexing feature comprises a peripheral rim (411) surrounding the inlet.
  • the peripheral rim 411 comprises a lip extending radially outward from the cuvette.
  • the purpose of the indexing feature is to index the cuvette against a hard stop that prevents movement of the cuvette.
  • the indexing feature is connected to the first and second walls of the cuvette.
  • the indexing feature as shown in FIG. 4B, is positioned at the distal end of the interrogation region.
  • the inlet is open directly to atmosphere and is sized appropriately to allow a sample or reagent to flow through the inlet from a pipettor or any other vessel such as a cartridge.
  • the width of the inlet is sized appropriately such that a pipettor can easily access the inlet opening.
  • the inlet opening is between 3 and 10 mm in width.
  • the opening of the inlet and the inlet chamber are sized appropriately such that the interrogation region of the cuvette may be filled under the influence of gravity, with fluid without creating air bubbles in the interrogation region, and so that fluids may be mixed e.g., by mechanical stirring.
  • the cuvette, (200) includes a body 203 connected to the inlet end.
  • the body is composed of walls surrounding the cuvette, with each wall having an inner surface (204) and an outer surface
  • the body portion of the cuvette includes an inlet chamber
  • the body portion includes a necked region (209) having an output channel (240).
  • the necked region has a restrictive channel, (210), wherein the inner diameter of the restrictive channel is smaller than that of the output channel.
  • the inlet chamber comprises an interrogation region (207) that includes a proximal end and a distal end.
  • the proximal end (213) of the interrogation region is positioned near or at the inlet (201) end of the cuvette, while the distal end (214) of the interrogation region is positioned near or at the beginning of the tapered region (208).
  • the walls at the interrogation region include a first wall and a second wall opposite the first wall, which, depending on the position of a light source, allows for transmission of light through the first wall and the second wall of the interrogation region.
  • the first and second walls of the interrogation region are substantially flat (see e.g., walls proximal to the interrogation region in FIG. 2).
  • the interrogation region is designed and dimensioned to accept light from an emitter (314) from one side of the cuvette and allow it to pass through the cuvette (300) to a light detector (315) on the opposite side of the cuvette without obstruction.
  • the light source (314) is closest to the first wall (left-most wall 305 of FIG. 3)
  • light will first transmit through a first wall 305, through the center of the interrogation region of the cuvette, and then through a second wall (right-most wall 316 of FIG. 3).
  • an optical detector (315) is situated proximal to the second wall (316).
  • FIG. 3 also shows a cone of light (317) transmitted through the interrogation region.
  • the optical path length of the interrogation region ranges from 1-20 mm, such as 1-10 mm, 5-10 mm, 7-10 mm, 10-15 mm, 5-12 mm, and 8-15 mm.
  • the optical path length of the interrogation region is 1 mm or more, 1.5 mm or more, 2 mm or more, 2.5 mm or more, 3 mm or more, 3.5 mm or more, 4 mm or more, 4.5 mm or more, 5 mm or more, 5.5 mm or more, 6 mm or more, 6.5 mm or more, 7 mm or more, 7.5 mm or more, 8 mm or more, 8.5 mm or more, 9 mm or more, 9.5 mm or more, 10 mm or more, 10.5 mm or more, 11 mm or more, 11.5 mm or more, 12 mm or more, 12.5 mm or more, 13 mm or more, 13.5 mm or more, 14 mm or more, 14.5 mm or more, 15 mm or more, 15.5 mm or more, 16 mm or more, 16.5 mm or more, 17 mm or more, 17.5 mm or more, 18 mm or more, 18.5 mm or more, 19 mm or more, or
  • Path lengths will vary depending on the assay being used. For example, a longer optical path length may be optimal for assays with lower concentrations of the sample. In contrast, a shorter path length may be optimal for assays with higher concentrations of the sample. As another non-limiting example, a shorter path length may be optimal for assays that require a high level of light absorbance.
  • the body (403) of the cuvette (400) further includes a tapered region (408) .
  • the tapered region (408) includes a proximal end and a distal end as shown in FIG. 4A.
  • the proximal end (418) is positioned near or closest to the distal end of the interrogation region (407), while the distal end (419) of the tapered region is positioned near or closest to the outlet (402) or an optional necked region (409) of the cuvette.
  • the tapered region (408) is positioned at the distal end of the interrogation region (407), with the inner surface of the wall at the tapered region tapering inward from the proximal end of the tapered region (408) to a narrowed section at the distal end (419) of the tapered region.
  • the tapered region (408) is between the interrogation region (407) surfaces and the outlet (402) of the cuvette.
  • This tapered transition region can include a connection point (412) shaped to promote fluid-elimination when the cuvette is drained from the outlet (402).
  • the tapered transition region creates a smooth transition from the interrogation region (407) to the neck of the cuvette, thereby eliminating corners and crevices that might otherwise cause fluid to become trapped when it is drained from the cuvette.
  • an obtuse angle (212 and 412) is formed between the inner surface of the first and second walls (204 and 221) at the tapered region (208) and the inner surface of the first wall (204) and second wall (221) at the interrogation region (207).
  • This obtuse angle provides for complete drainage of the sample from the cuvette once the sample is measured.
  • the obtuse angle between the inner surface of the wall of the tapered region and the inner surface of the first wall and the second wall of the interrogation region ranges from 130 to 179 degrees.
  • the obtuse angle between the inner surface of the wall of the tapered region and the inner surface of the first wall and the second wall of the interrogation region can be at least 130 degrees, at least 135 degrees, at least 140 degrees, at least 145 degrees, at least 150 degrees, at least 155 degrees, at least 160 degrees, at least 165 degrees, at least 170 degrees, or at least 175 degrees.
  • the cuvette comprises a connection point (412) between the distal end of the interrogation region and the proximal end of a tapered region, wherein the connection point comprises the obtuse angle ranging from 130 to 179 degrees.
  • the obtuse angle prevents liquid from getting trapped through the surface energy interaction with the walls of the cuvette and thus allows for fluid to flow down the cuvette.
  • the cuvette further comprises fillets and/or chambers on all internal surfaces of the cuvette to eliminate sharp edges or corners where liquid can pool.
  • the body of the cuvette further comprises a necked region
  • the necked region (509) allows for sealing to a manifold (523).
  • the necked region may be used to create a radial seal with a manifold (523) through use of an O-ring (524).
  • the necked region contains a channel that comprises an outlet (202) at the distal end of the necked region.
  • the necked region contains an elongated outlet channel as shown in FIGs. 2 and 4.
  • the necked region contains a short outlet channel as shown in FIG. 7.
  • the short necked region that includes a channel comprises a length ranging from 0 to 2 mm.
  • the long necked region comprises a length ranging from 2 to 10 mm.
  • the necked region comprising a channel comprises an inner diameter at the proximal end of the channel that is smaller than at the distal end of the channel.
  • the necked region comprises an outlet channel (240) and a restrictive channel (210) that are in series with each other.
  • the outlet channel comprises an inner diameter at the proximal end of the outlet channel that is smaller than the distal end of the outlet channel.
  • the outlet channel comprises an inner diameter at the proximal end of the outlet channel that is bigger than the distal end of the outlet channel.
  • the restrictive channel comprises an inner diameter at the proximal end of the restrictive channel that is smaller than the distal end of the restrictive channel.
  • the restrictive channel comprises an inner diameter at the proximal end of the restrictive channel that is bigger than the distal end of the restrictive channel.
  • the necked region comprises a restrictive channel that is between 0.5 and 4mm in length, and has an inner diameter between 0.1 mm and 1 mm.
  • the necked region of the cuvette further comprises an outlet channel that is fluidically connected to the restrictive channel having a smaller diameter than the outlet channel.
  • the fluidic channel(s) of the necked region create a passage between the tapered region of the cuvette and the outlet.
  • the restrictive channel (210) creates a passage between the tapered region (208) of the cuvette and the outlet channel (240).
  • the restrictive channel (210) of the cuvette is created by inserting a restrictive element into the necked region of the cuvette, the restrictive element having an opening that is between 0.1 mm and 1mm in diameter, and a length ranging between 0.5 and 4 mm.
  • the tapered region of the cuvette gradually narrows to a small channel with a smaller inner diameter of the necked region (FIG 2D). In other embodiments, there is a sudden step change from the tapered region of the cuvette to the small inner diameter channel of the necked region. (FIG 2E)
  • the restrictive channel comprising a smaller first inner diameter positioned proximal to the tapered region of the cuvette exhibits a step change to an outlet channel with a larger internal diameter (FIG 2C).
  • the necked region comprises a restrictive channel with a minimum inner diameter ranging from 0.01 mm to 2 mm. In some embodiments, the necked region comprises a restrictive channel with an inner diameter ranging from 0.01 mm to 1 mm. In some embodiments, the necked region comprises a restrictive channel with an inner diameter ranging from 0.01 mm to 0.5 mm. In some embodiments, the necked region comprises a restrictive channel with an inner diameter ranging from 0.2 mm to 1 mm.
  • the necked region comprises a restrictive channel with an inner diameter of 0.01 mm, 0.05 mm, 0.06 mm, 0.07 mm, 0.08 mm, 0.09 mm, 0.1 mm, 0.15 mm, 0.2 mm, 0.25 mm, 0.3 mm, 0.35 mm, 0.4 mm, 0.45 mm, 0.5 mm, 0.55 mm, 0.6 mm, 0.65 mm, 0.7 mm, 0.75 mm, 0.8 mm, 0.85 mm, 0.9 mm, 0.95 mm, 1 mm, 1.1 mm, 1.2 mm, 1.3 mm, 1.4 mm, 1.5 mm, 1.6 mm, 1.7 mm, 1.8 mm, 1.9 mm, or 2 mm. 5.2.2.3.1 Cuvette Outlet Channel
  • the cuvette of the present disclosure comprises an outlet channel that includes an outlet (202).
  • the outlet of the cuvette is configured to allow the sample to exit the cuvette.
  • the outlet is positioned at the distal end of the outlet channel.
  • the inner diameter of the outlet (402) (or inner diameter of the outlet channel) is sufficiently small to prevent fluid from passing through the outlet (402) during measuring and/or processing of the sample.
  • the inner diameter of the outlet (402) is configured to cause surface tension to keep the fluid in the inlet chamber.
  • the size of the outlet (402) is configured so that the upward force due to the capillary pressure between the fluid/ solid and fluid/air interfaces at the outlet 402 are equal to the gravitational force of the fluid above the outlet (e.g., in the interrogation region), thus preventing the fluid from draining from the cuvette under the influence of gravity.
  • the surface tension of cuvette outlet 202 is configured to maintain fluid in the interrogation region of the cuvette.
  • the internal diameter (inner diameter) of the outlet channel is configured to elicit capillary force at the outlet channel that is equal to the gravitational force of the fluid above the outlet, thus maintaining the fluid in the inlet chamber and preventing the fluid from draining from the cuvette under the influence of gravity.
  • the surface energies between the liquid/solid and liquid/air interfaces at the outlet channel is configured to prevent the fluid from draining from the cuvette under the influence of gravity.
  • both the size of the inner diameter of the outlet channel and the surface energies between the liquid/solid and liquid/air interface at the outlet channel are configured to prevent the fluid from draining from the cuvette under the influence of gravity.
  • the outlet end of the cuvette is configured to be deformable such that it is capable of opening and closing.
  • the cuvette may be fabricated, e.g. by injection molding, or machining, so that the outlet channel in the necked region of the cuvette has a relatively large inner diameter (e.g. 1 - 2 mm).
  • the outlet channel may be further constricted by press-fitting or gluing a short length of a restrictive element (such as a short length of drawn tubing) into the neck, as illustrated in FIG 6.
  • This method permits the outlet of each cuvette to be adjusted to any desired inner diameter, based on factors that may include the viscosity and surface tension of the liquids contained therein. Furthermore, it reduces the complexity of the injection molding or machining operations necessary to fabricate the cuvettes.
  • the necked region of the cuvette comprises a monolithic restrictive channel as illustrated in FIG 2.
  • the outlet (or outlet channel) comprises an opening with an inner diameter ranging from 0.01 mm to 2 mm. In some embodiments, the outlet comprises an opening with an inner diameter ranging from 0.01 mm to 1 mm. In some embodiments, the outlet comprises an opening with an inner diameter ranging from 0.01 mm to 0.5 mm. In some embodiments, the outlet comprises an opening with an inner diameter ranging from 0.2 mm to 1 mm.
  • the outlet comprises an opening with an inner diameter of 0.01 mm, 0.05 mm, 0.06 mm, 0.07 mm, 0.08 mm, 0.09 mm, 0.1 mm, 0.15 mm, 0.2 mm, 0.25 mm, 0.3 mm, 0.35 mm, 0.4 mm, 0.45 mm, 0.5 mm, 0.55 mm, 0.6 mm, 0.65 mm, 0.7 mm, 0.75 mm, 0.8 mm, 0.85 mm, 0.9 mm, 0.95 mm, 1 mm, 1.1 mm, 1.2 mm, 1.3 mm, 1.4 mm, 1.5 mm, 1.6 mm, 1.7 mm, 1.8 mm, 1.9 mm, or 2 mm.
  • the outlet comprises an outer diameter (the outer-diameter of the necked region) ranging from 0.25 mm to 6 mm. In some embodiments, the outlet comprises an outer diameter ranging from 0.25 to 5 mm. In some embodiments, the outlet comprises an opening with an outer diameter ranging from 0.25 mm to 2 mm. In some embodiments, the outlet comprises an opening with an outer diameter ranging from 0.25 mm to 1.5 mm. In some embodiments, the outlet comprises an opening with an outer diameter ranging from 1.5 mm to 2 mm. In some embodiments, the outlet comprises an opening with an outer diameter of 2 mm, 2.5 mm, 3 mm, 3.5 mm, 4 mm, 4.5 mm, 5 mm, 5.5 mm, or 6 mm.
  • the cuvette, (600) includes a restrictive channel (510).
  • the restrictive channel is positioned/connected to the distal end of the tapered region and the outlet channel of the cuvette.
  • the combination of the restrictive channel and the abrupt transition (expansion or contraction) of the channel diameter constitutes a passive capillary valve (530).
  • the cuvette comprises a capillary valve, e.g., between the transition in diameter of the distal end of the tapered region and the diameter of the proximal end of the restrictive channel.
  • 5B illustrates the forces on the fluid when the liquid has filled the cuvette up to the opening of the restrictive channel (510) in the necked region (509).
  • the force of gravity acts to push the fluid down with a force proportional to the height of the fluid in the cuvette and the cross-sectional area of the outlet channel in the restrictive channel (510).
  • An upward force also acts on the fluid, proportional to the surface tension of the fluid/air interface, the radius of the restrictive channel (510), and dependent upon the abrupt angle with which the transition from large to small diameter occurs.
  • the equilibrium position of the fluid is at the entry to the restrictive channel, and all liquid is maintained in the inlet chamber of the cuvette.
  • the surface tension of the fluid/air interface increases, or the radius of the restrictive channel (510) decreases, the surface tension of the fluid/air interface becomes relatively more important than gravity, and the height of the fluid that can be maintained in the cuvette increases.
  • FIG. 5C shows a state in which the capillary pressure associated with the passive valve is insufficient to keep the liquid in the inlet chamber. Liquid therefore enters the restrictive channel in the necked region. As the liquid enters the restrictive channel, it compresses the air trapped between the liquid and a downstream active valve. The additional pressure generated by the compression of air adds to the capillary force associated with the liquid/air meniscus in the restrictive channel to create a force that opposes further egress of the liquid from the inlet chamber.
  • FIG. 5D shows a state in which liquid has continued to move out of the inlet chamber until it reaches the end of the restrictive channel.
  • this state there is a second abrupt transition from a small diameter channel to a large diameter channel.
  • This second transition constitutes a second capillary valve (532), and generates an additional capillary pressure that resists further egress of the liquid from the cuvette.
  • the air trapped between the liquid and the active valve has been further compressed, resulting in additional force that resists the egress of liquid from the cuvette.
  • the cuvette comprises a second capillary valve, e.g., between the transition in diameter of the distal end of the restrictive channel and the diameter of the proximal end of the outlet channel.
  • the passive valve (530) is created by insertion of a restrictive element into the necked region of the cuvette.
  • a restrictive region is shown as a restrictive element (625) in FIG. 6.
  • the restrictive element has an opening that is between 0.1 mm and 1 mm in diameter.
  • the volume of air between the inlet chamber of the cuvette and the closed active valve ranges from 5 and 60 microliters. In some embodiments, the volume of air between the inlet chamber of the cuvette and the closed active valve is 50 microliters or less, 40 microliters or less, 30 microliters or less, 20 microliters or less, 10 microliters or less, 9 microliters or less, 8 microliters or less, 7 microliters or less, 6 microliters or less, or 5 microliters or less.
  • the necked region has a passive valve (e.g., a restrictive channel) with a diameter small enough that liquid dropped or dispensed into the inlet of the cuvette does not fill or only partially fills the restricted opening of the restrictive channel in the necked region.
  • a passive valve e.g., a restrictive channel
  • the force that prevents liquid from displacing air in the restricted opening of the necked region is a capillary force.
  • it is the force that results from the geometric shape of the opening in the outlet, and from the surface tension between the liquid/solid interface.
  • the force keeping the liquid from displacing air in the restricted opening of the necked region is the force due to the compression of the air that is trapped between the liquid in the cuvette and a downstream active valve.
  • a combination of capillary and compressed-air forces is configured to keep the air in the restricted opening of the necked region from being displaced.
  • the restrictive channel of the cuvette creates a passive capillary valve.
  • the outlet of the cuvette is in fluidic communication with a tube or channel that can be opened and closed through means of an active valve.
  • an active valve placed downstream of the cuvette may open and close, such that opening the active valve creates a fluid path from the outlet of the cuvette to a separate channel downstream of the valve, and closing the valve disconnects the outlet of the cuvette from the downstream channel and traps a small volume of air between the closed valve and the liquid in the cuvette.
  • the volume of air trapped between liquid in the cuvette and the active valve is small, such that the head pressure of the liquid in the cuvette is substantially smaller than the increase in the air pressure that would result from the compression of air if fluid was to pass through the outlet channel of the cuvette comprising a small inner diameter.
  • the pressurized air trapped between the active valve and liquid in the cuvette maintains the liquid in the inlet chamber of the cuvette and prevents the liquid from draining from the cuvette under the influence of gravity.
  • the volume or pocket of air is trapped between the liquid in the inlet chamber of the cuvette and the active valve. In some embodiments, the volume or pocket of air is trapped between liquid in the restrictive channel and the active valve. In some embodiments, the volume or pocket of air is trapped between liquid in the outlet channel and the active valve.
  • the equilibrium position of the liquid/air meniscus in the restricted channel of the necked region is located at the point where the forces due to gravity, capillary pressure, and air-pressure balance one another.
  • the choice of cuvette material and the type of liquid put into the cuvette will influence the capillary pressure.
  • the cuvette shape and the volume of liquid put into the cuvette will change the height of the column of liquid in the cuvette, and so alter the force due to gravity.
  • the volume of air trapped between liquid in the cuvette inlet chamber and an active valve influences the rate at which pressure increases when the air from the restrictive channel is displaced.
  • the cuvette and associated hardware e.g. the location of the active valve
  • an exemplary cuvette of the present disclosure can hold a liquid column that is up to 2 cm in height.
  • the head-pressure associated with a 2 cm tall liquid column of an aqueous solution is approximately 0.03 psi.
  • the volume of air trapped between the liquid in the cuvette and a downstream active valve is 50 microliters. When this volume is compressed to 49.9 microliters, an air pressure of 0.03 psi is generated. In other words, a change in the volume of air of just 0.1 microliters is sufficient to counteract a 2 cm head pressure.
  • restrictive channel in the cuvette has an internal volume greater than 0.1 microliters, liquid will not fill the restrictive channel under the influence of gravity.
  • a restrictive channel having an inner diameter of 0.5mm and a length of 1mm has an internal volume of 0.19 microliters, which is almost twice the volume necessary to ensure that no liquid passes through the restrictive channel.
  • the restrictive channel short so that it is easy to manufacture by molding, and so that the cuvette can be emptied quickly when a pressure drop is applied across the restrictive channel. In some embodiments, it is preferable to keep the volume of air between the inlet chamber and the active valve small.
  • the length of the restrictive channel is at least 1mm. In some embodiments, the length of the restrictive channel ranges from 0.05 mm to 8 mm. In some embodiments, the restrictive channel comprises a length ranging from 0.5mm to 5 mm. In some embodiments, the length of the restrictive channel ranges from 0.05 mm to 5 mm. In some embodiments, the length of the restrictive channel is at least 0.05 mm, at least 0.1 mm, at least 0.5 mm, at least 1 mm, at least 1.5 mm, at least 2 mm, at least 3 mm, at least 4 mm, at least 5 mm, at least 6 mm, at least 7 mm, at least 8 mm, at least 9 mm, or at least 10 mm.
  • the restrictive channel is configured to hold a volume of fluid ranging from 0.05 microliters to 1 microliter. In some embodiments, the restrictive channel is figured to hold a volume of fluid ranging from 0.01 microliters to 2 microliters. In some embodiments, the restrictive channel is configured to hold a volume of fluid ranging from 0.01 microliters to 1 microliter. In some embodiments, the restrictive channel is configured to hold a volume of 2 microliters or less, 1 microliter or less, 0.5 microliters or less, 0.4 microliters or less, 0.2 microliters or less, or 0.1 microliters or less.
  • An aspect of the cuvette that is the subject of this disclosure is that in operation, a substantial part of the interrogation region is filled with fluid without introducing air bubbles that might obscure the optical path of light used for photometric measurements. Air in the path of light can lead to light diffraction, reflection, and scatter, thereby causing errors in the measurement of transmitted light.
  • the design and dimensions of the cuvette of the present disclosure are such as to prevent the entrapment of bubbles in the interrogation region and to ensure that all the light collected by the detector is representative of the fluids contained within the cuvette.
  • the cuvette is dimensioned such that every portion of light collected by the photodetector has passed through the liquid in the cuvette. If this condition is not observed, the background noise associated with the measurement is increased and the measurement system will have reduced sensitivity at the lower end of the sampleconcentration range.
  • a path length for light propagating from a source of light through the fluids being measured to a detector.
  • Typical photometric modules are structured to ensure that the path length is on the order of 1 cm.
  • Some point-of-care blood analysis instruments may be configured to utilize path lengths as small as a few hundred microns.
  • the path length of the interrogation region of the cuvette ranges from 1 mm to 5 mm. In some embodiments, the path length of the interrogation region of the cuvette ranges from 1 mm to 25 mm.
  • the path length of the interrogation region of the cuvette ranges from 1 micron to 1000 microns. [176] In some embodiments, the volume of the interrogation region of the cuvette is greater than 50% of the total volume of fluid that fills or is placed into the cuvette.
  • the minimum operational value of the cuvette width over which light is transmitted is determined both by the desire to measure the analyte having the lowest concentration (at the dilution ratio of the assay) and by the availability of the sample to be measured.
  • the amount of fluid required to fill the interrogation region also increases. If sample is limited, it may be necessary to dilute the sample to achieve this fill. However, if the dilution ratio is made too large, it may not be possible to detect analytes at low-concentration.
  • the necessary sample dilution ratio for an assay also decreases, (e.g., a more concentrated sample may be used). However, if the width of the cuvette results in an optical path length that is too small, it will again be impossible to detect analytes at low-concentration.
  • the volume of liquid that interacts with the light as it passes through the cuvette is large, because this volume is directly proportional to the sensitivity of the assay.
  • the total volume of liquid used in the cuvette is the minimum volume needed to perform the assay so as to reduce waste and minimize cost.
  • the cuvette of the present disclosure is configured to hold a total volume ranging from 50 microliters to 5 milliliters. In some embodiments, the cuvette of the present disclosure is configured to hold a total volume ranging from 50 microliters to 4 millimeters.
  • the cuvette of the present disclosure is configured to hold a total volume ranging from 50 microliters to 3 mililiters. In some embodiments, the cuvette of the present disclosure is configured to hold a total volume ranging from 50 microliters to 2 milliliters. In some embodiments, the cuvette of the present disclosure is configured to hold a total volume ranging from 50 microliters to 1 milliliter. In some embodiments, the cuvette of the present disclosure is configured to hold a total volume ranging from 50 microliters to 800 microliters. In some embodiments, the cuvette of the present disclosure is configured to hold a total volume ranging from 50 microliters to 500 microliters.
  • the cuvette of the present disclosure is configured to hold a total volume ranging from 50 microliters to 400 microliters. In some embodiments, the cuvette of the present disclosure is configured to hold a total volume ranging from 50 microliters to 300 microliters. In some embodiments, the cuvette of the present disclosure is configured to hold a total volume ranging from 50 microliters to 200 ml. In some embodiments, the cuvette of the present disclosure is configured to hold a total volume ranging from 50 microliters to 100 microliters.
  • the cuvette body is composed of walls surrounding the cuvette.
  • the cuvette body can include a short wall (830) on one side and a long wall (831) on the opposite side.
  • a cuvette may be positioned with the short side wall (830) that is proximal to the light source and the long side wall (831) that is proximal to the optimal detector, so that the cuvette widens in the same or similar manner as the widening of the beam of light as it passes from the light source to the detector.
  • a non-limiting example of such a cuvette with a long side wall (831) and a short side (830) wall is a cuvette that has a trapezoidal cross-section, as illustrated in FIG. 8A-8C.
  • a cuvette with a trapezoidal cross-section (FIG. 8B) includes a smaller total liquid volume but the amount of liquid that interacts with the light entering the cuvette remains the same.
  • the obtuse angles of the trapezoid may be of any value between 90° and 180° but are preferably equal -to or slightly larger than the angle of the cone of light emitted by the light source.
  • cross-sectional geometries are possible to maintain the function and parameters of the cuvette in a cuvette module.
  • other cross-sectional geometries such as a square cross-section also prevent liquid within the interrogation region of the cuvette from exiting the cuvette until an additional pressure gradient is applied across the cuvette outlet.
  • the cuvette or portions thereof is composed of a plastic material.
  • the plastic material is selected from: polycarbonate, polystyrene, acrylic, polypropylene, cyclic-olefin-copolymer, cyclic-olefin-polymer, copolyester, polyethylene terephthalate (PET), polyethylene terephthalate glycol (PETG), or other transparent plastic such as are known in the art.
  • the cuvette is molded from an injection moldable plastic.
  • the cuvette or portions thereof are composed of glass, quartz, or fused-silica. [183] In some embodiments, the cuvette or portions thereof (e.g., walls of the cuvette) is composed of a transparent elastomer.
  • the cuvette or portions thereof e.g., walls of the cuvette
  • the cuvette or portions thereof is composed of a thin, transparent material to enable light to pass through the cuvette from one side to another.
  • the cuvette, or portions thereof, is made from a material that has a static liquid/solid contact angle greater than 90°for all fluids that are to be placed inside the cuvette.
  • the restricted channel of the cuvette is made from a different material than that of the rest of the cuvette.
  • the restricted channel is made of PEEK, FEP, PTFE, or PF A plastic.
  • the cuvette may be dyed, colored, or coated, with a wavelength-selective material.
  • the cuvette comprises one or more materials molded into the materials used to make the cuvette in order to change the optical properties of the light passing through the cuvette.
  • materials embedded into the cuvette materials can include a dye, coating, or a lens molded into the cuvette.
  • the cuvette dye, color, or coating acts as a light filter.
  • dyes of any color may be added to the cuvette during the manufacturing process, as illustrated in FIG 9A
  • the cuvette is coated with one or more coatings as shown in FIG 9B. As shown in FIG. 9B, each wall of the cuvette can be coated with the same or different coating/color. These dyes and coatings may serve to alter the spectrum, polarity, or intensity of light transmitted through the cuvettes.
  • the cuvette, or portions thereof is made from a material that has a water/solid contact angle that is less than 90 degrees.
  • FIG. 4B shows an alternative embodiment of the cuvette of the present disclosure.
  • the cuvette, (400) is substantially the same as the cuvette of FIG. 2.
  • the peripheral rim (411) that may serve as an indexing feature is here located between the distal end of the interrogation region (407) and the proximal end of the tapered region (408).
  • the cuvette further comprises an indexing feature, wherein the indexing feature comprises a peripheral rim (411) positioned between the distal end (414) of the interrogation region and the proximal end (418) of the tapered region, as shown in FIG. 4B.
  • FIG. 7 shows another embodiment of the cuvette of the present disclosure.
  • This cuvette (700) shares many common features with cuvettes of FIGs. 2-6. However, this cuvette has a much shorter necked region (709) as compared to cuvettes of FIGs. 2-6, and includes an axial-gasket plate (732).
  • the axial-gasket plate (732) could also serve as an indexing feature, making the peripheral rim unnecessary.
  • the cuvette comprising the axial-gasket plate does not comprise a peripheral rim.
  • FIG. 10 shows another embodiment of the cuvette of the present disclosure.
  • This cuvette, (1000) is made from a soft elastomeric tube.
  • the tube has a square cross-section and has substantially flat walls.
  • the tube is cylindrical, but is inserted into a housing that causes at least two of the opposing walls to deform into substantially flat walls.
  • the cuvette of FIG. 10 is connected to a pinching element (1033), such as a pinch valve, or a peristaltic pump that, when closed, creates a tapered region and a closed outlet for containing the fluid within the cuvette during processing and/or measurement of the fluid.
  • a pinching element (1033) such as a pinch valve, or a peristaltic pump that, when closed, creates a tapered region and a closed outlet for containing the fluid within the cuvette during processing and/or measurement of the fluid.
  • the interrogation region of the cuvette of FIG. 10 is contained within the inlet chamber (1006), and may be filled under the influence of gravity by a fluid inserted through the inlet (e.g. by a pipettor). Further, the outlet (1002) of the cuvette of FIG. 10 is constrained to have an opening that is small enough to prevent fluid from exiting the cuvette under the influence of gravity alone.
  • a cuvette for holding fluids comprising: (A)an inlet end comprising an inlet open to the atmosphere, wherein the inlet is configured to receive the fluids dropped or dispensed into the inlet from above the cuvette; (B) a body of the cuvette connected to the inlet end, the body composed of walls surrounding the cuvette, each wall having an inner surface and an outer surface, the walls including a plurality of regions comprising an inlet chamber and a necked region; (C) the inlet chamber configured to be filled under the influence of gravity by the fluid dropped or dispensed into the inlet; (D) the inlet chamber containing an interrogation region comprising a proximal end and a distal end, the proximal end positioned at the inlet end of the cuvette, the walls at the interrogation region including a first wall and a second wall opposite the first wall, wherein the inlet chamber is configured to allow transmission of light through the first wall and the second wall of the interrog
  • the various embodiments of the cuvette of the present disclosure may be ganged together to create structures containing a plurality of cuvettes (e.g. a multiplicity of cuvettes or an array of cuvettes). For instance, as shown in FIG. 11, individual cuvettes may be placed into ID or 2D arrays, or may be assembled into other structures, such as rings. Furthermore, arrays of cuvettes may be fabricated as a monolithic block, as illustrated in FIG. 11C.
  • the plurality of cuvettes comprises at least 2 cuvettes, at least 3 cuvettes, at least 4 cuvettes, at least 5 cuvettes, at least 6 cuvettes, at least 7 cuvettes, at least 8 cuvettes, at least 9 cuvettes, or at least 10 cuvettes.
  • the plurality of cuvettes comprises at least 5 cuvettes, at least 10 cuvettes, at least 15 cuvettes, at least 20 cuvettes, at least 25 cuvettes, at least 30 cuvettes, at least 35 cuvettes, at least 40 cuvettes, at least 45 cuvettes, or at least 50 cuvettes.
  • aspects of the present disclosure include a cuvette module for processing and performing measurements, such as photometric measurements.
  • the cuvette module is a multiplexed device that can process and measure multiple samples in a single cuvette or in an array of cuvettes within the cuvette module.
  • the cuvette module comprises at least one compartment capable of holding at least one cuvette. In some embodiments, the cuvette module comprises one or more compartments capable of holding at least one cuvette. In some embodiments, the cuvette module comprises at least one cuvette. In some embodiments, the cuvette module comprises one or more cuvettes.
  • FIG. 12 shows a cuvette module that includes one or more compartments configured to hold at least one cuvette of the present disclosure.
  • the cuvette has has an inlet open to the atmosphere, wherein the inlet is configured to receive fluids dropped or dispensed into the inlet from above the cuvette.
  • the cuvette further comprises an inlet chamber that holds the fluids.
  • the cuvette further comprises an output, the output having a restrictive channel. Any of the cuvette designs described in the present disclosure can be used in the cuvette module of the present disclosure.
  • the cuvette module comprises a cuvette and a housing comprising one or more housing walls shaped to hold and mechanically constrain the cuvette in place.
  • the cuvette module also includes a manifold having a fluidic channel positioned between and fluidically connecting the outlet of the cuvette and an active valve (528).
  • the active valve (528) can serve to open and close the connection between the outlet (502) of the cuvette and a downstream channel (529).
  • the cuvette module comprises an array of valves that serve to open and close specifically selected cuvettes when needed.
  • the cuvette module of the present disclosure comprises one or more cuvettes described herein.
  • the cuvette module comprises 2 or more cuvettes, 3 or more cuvettes, 4 or more cuvettes, 5 or more cuvettes, 6 or more cuvettes, 7 or more cuvettes, 8 or more cuvettes, 9 or more cuvettes, 10 or more cuvettes, 15 or more cuvettes, 20 or more cuvettes, 25 or more cuvettes, 30 or more cuvettes, 40 or more cuvettes, 45 or more cuvettes, or 50 or more cuvettes.
  • cuvette module the 2 or more cuvettes are positioned in an array within the cuvette module as shown in FIG. 13.
  • one or more cuvettes are reusable.
  • the one or more cuvettes comprise at least 2 reusable cuvettes, at least reusable 3 cuvettes, at least 4 reusable cuvettes, at least reusable 5 cuvettes, at least reusable 6 cuvettes, at least reusable 7 cuvettes, at least reusable 8 cuvettes, at least reusable 9 cuvettes, or at least 10 reusable cuvettes.
  • the array of cuvettes comprises 2-10 reusable cuvettes, 2-20 reusable cuvettes, 2-50 reusable cuvettes, or 2-100 reusable cuvettes.
  • the cuvette module contains an array of cuvettes and associated hardware.
  • the cuvettes may be individual cuvettes inserted into individual cavities in the housing.
  • the cuvettes may be joined together into a monolithic array which is positioned in the housing.
  • the cuvette module comprising the cuvette of the present disclosure further comprises a housing into which one or more cuvettes is inserted or placed.
  • the housing includes an opening at a proximal end of the housing walls for inserting the cuvette into the housing.
  • the cuvette module further comprises a housing having one or more housing walls shaped to hold and mechanically constrain at least one cuvette and orient it so that its inlet chamber may be filled under the influence of gravity.
  • the cuvette module further comprises a housing cap (140) that additionally constrains the position of the cuvette. In some embodiments, the cap may be attached to the housing over a portion of the cuvette.
  • FIG. 15A shows a cuvette comprising a long-necked region of different designs fitted into a housing of the cuvette module and fluidically connected through a radial seal
  • FIG. 15B shows a cuvette design with a short-necked region fitted into a housing of the cuvette module and fluidically connected through an axial seal.
  • the housing comprises a slot on at least one of the housing walls to register or index the cuvette in a selected position within the housing.
  • the lip or indexing feature of cuvette is positioned such that when the cuvette is securely placed into the housing, the lip or indexing feature of the cuvette is configured to stop the cuvette from movement further into the housing (see e.g., FIG. 15A and FIG. 15B).
  • the housing comprises a fastener on at least one of the housing walls to fasten the cuvette into the housing.
  • a cuvette module comprises an array of cuvettes
  • each cuvette within the array of cuvettes is positioned in a separate opening or slot within the housing for inserting and indexing each cuvette.
  • the cuvette module of the present disclosure incorporates a fluidic seal that puts the outlet of the cuvette in fluid communication with a fluidic tube or channel (e.g., central channel described herein as 527), and an active valve (528) that can open and close the path from the cuvette to the downstream tube or channel (529).
  • a fluidic tube or channel e.g., central channel described herein as 527
  • an active valve e.g., an active valve that can open and close the path from the cuvette to the downstream tube or channel (529).
  • the fluidic seal puts the cuvette in fluidic communication with a manifold; the manifold having a fluidic channel positioned between and fluidically connecting the outlet of the cuvette and an active valve that serves to open and close the connection between the outlet of the cuvette and a downstream channel.
  • the housing comprises a fluidic seal around one or more regions of the cuvette wall.
  • a fluidic seal can be positioned within the housing at the necked region, of the cuvette.
  • a fluidic seal can be positioned at the distal end of the transition tapered region.
  • the fluidic seal is a radial seal.
  • the fluidic seal is an axial seal. In some embodiments, the fluidic seal comprises an O-ring or a gasket. In some embodiments, the fluidic seal is configured to hold the cuvette in place within the housing. In some embodiments, the housing comprises a gasket positioned around the outlet end of the cuvette.
  • the housing further comprises a housing cap as shown in FIG. 12 and FIG. 14.
  • the housing cap does not constrict or block the inlet opening of the cuvette, but sits around the edges of the proximal end of the inlet and allows for holding the cuvette in place.
  • the housing cap (140) is also shown in FIG. 14 and FIG. 16.
  • the cuvette module may comprise an array of caps, or a single cap may be configured so that it covers the entire array.
  • the cap(s) may have holes in them corresponding in size and location to the cuvette inlet openings, so that when the cap is placed on top of the cuvettes, it does not prevent fluid from being loaded into the cuvettes.
  • the cap may be used to mechanically- secure the cuvettes in their housing. For example, the cap may make contact with the top surface of a peripheral rim surrounding the inlet end of the cuvette, and may be screwed down to the housing so that it prevents the cuvettes from being unintentionally withdrawn from the housing.
  • the cuvette module further comprises a manifold.
  • the manifold includes a fluidic channel positioned between and fluidically connecting the outlet of the cuvette and a fluidic valve.
  • the manifold includes a neck chamber positioned between the fluidic channel and the outlet of the cuvette, as shown in FIG. 15 A.
  • the manifold of the present cuvette module can be of a size appropriate to fluidically connect a single cuvette or an array of cuvettes to a single active fluidic valve or an array of active fluidic valves.
  • the manifold comprises a number of fluidic channels and valves that correspond to the number of cuvettes in the cuvette module.
  • the manifold comprises one or more fluidic channels, 2 or more fluidic channels, 3 or more fluidic channels, 4 or more fluidic channels, 5 or more fluidic channels, 6 or more fluidic channels, 7 or more fluidic channels, 8 or more fluidic channels, 9 or more fluidic channels, 10 or more fluidic channels, 15 or more fluidic channels, 20 or more fluidic channels, 25 or more fluidic channels, 30 or more fluidic channels, 40 or more fluidic channels, 45 or more fluidic channels, or 50 or more fluidic channels, each fluidic channel positioned between and fluidically connecting a corresponding outlet of a cuvette and a fluidic valve.
  • the cuvette module 2 or more fluidic channels are positioned in an array within the cuvette module.
  • the manifold comprises a seal around one or more regions of the cuvette wall as shown in FIG. 12 and FIG. 15.
  • FIG. 15 illustrates two embodiments of the cuvette module; a first embodiment uses a radial O-ring (FIG. 15 A) as an element to create a fluidic seal between a cuvette and a manifold. A second embodiment uses an axial gasket (FIG. 15B) to accomplish the fluidic seal between the cuvette and the manifold.
  • a radial O-ring FIG. 15 A
  • FIG. 15B axial gasket
  • a seal can be positioned within the manifold and is adjacent to the necked region of the cuvette. In other embodiments, a seal can be positioned within the manifold and is adjacent to the distal end of the tapered region.
  • the manifold comprises a seal adjacent to the distal end of the outlet of the cuvette that seals the distal end of the outlet to a channel within the manifold.
  • the seal is a radial seal.
  • the seal is an axial seal.
  • the seal comprises an O-ring or a gasket. In some embodiments, the seal is configured to hold the cuvette in place within the housing.
  • the manifold comprises a gasket positioned around the outlet end of the cuvette.
  • the outlet end of the cuvette is configured to create a radial seal with the gasket.
  • the gasket is configured to provide an air-tight and/or fluid-tight seal between the manifold and the outlet of the cuvette.
  • the gasket is configured to provide an airtight and/or fluid-tight seal between the first fluidic channel and the outlet of the cuvette.
  • the seal creates a fluid-tight connection between the cuvette and the manifold.
  • the cuvette module further comprises a second gasket configured to provide an air-tight and/or fluid-tight seal between an active fluidic valve and the manifold. This is distinct from the gasket that creates a fluid seal between the cuvette module and the cuvette.
  • the fluidic manifold further comprises a waste channel (e.g., waste line) as shown in FIG. 16A.
  • waste line is fluidically connected to one or more active fluidic valves.
  • the module may also comprise a plurality of O-rings, gaskets, or other means of fluidically sealing the cuvettes to a manifold.
  • the manifold comprises fluidic channels that connect the output of each cuvette to a valve, to enable the output of each cuvette to be controllably opened and closed, and to enable a volume of air to be trapped between the valve and the liquid in each cuvette.
  • the cuvette module of the present disclosure comprises a fluidic valve (used interchangeably herein as “active fluidic valve” or “active valve”).
  • active fluidic valve used interchangeably herein as “active fluidic valve” or “active valve”).
  • the active valve is fluidically connected to the fluidic central channel (527) of the manifold.
  • the active valve is configured to open and close the connection between the central channel (527)and a downstream channel (529) of the manifold.
  • the cuvette module comprises an active valve fluidically connected to each fluidic channel. Each active valve allows the connection between the outlet of a specific cuvette and a downstream channel to be opened and closed.
  • the active fluidic valve (528) serves an additional function, for example, when closed, the active fluidic valve traps a small volume of air between the outlet (502) of the cuvette and the active valve (528) that can serve to help keep fluid in the cuvette inlet chamber if the first passive valve (530) is insufficient to counter the head pressure of the liquid in the cuvette (FIG. 5B and 5C).
  • the module when the active valve is closed, the module traps a volume of air between it and the liquid in the inlet chamber of the cuvette.
  • FIG. 15C illustrates the state of the module before liquid has been dropped or dispensed into the cuvette.
  • the cuvette and the tube are at atmospheric pressure.
  • FIG. 15D illustrates an embodiment in which liquid has been dropped or dispensed into the cuvette, thereby trapping a volume of air, VI, between the valve and the liquid.
  • 15E illustrates an alternative state wherein some of the liquid in the cuvette begins to pass into the restrictive channel of the cuvette, thereby compressing the trapped volume of air to a volume V2. In this state, the pressure of the trapped air again rises to be equal and opposite to the head pressure of the liquid.
  • FIG. 15F illustrates a state in which the cuvette is being emptied; the valve is opened and a downstream pump (not shown) pulls the fluid out of the cuvette.
  • the fluidic valve (active valve) comprises at least one port. In some embodiments, the fluidic valve comprises at least two ports. In some embodiments, the fluidic valve comprises at least three ports. In some embodiments, the fluidic valve is a oneway valve. In some embodiments, the fluidic valve is a two-way valve. In some embodiments, the fluidic valve is a three-way valve. In some embodiments, at least one port of the fluidic valve is connected to the central channel. In some embodiments, at least one port of the fluidic valve is connected to the downstream channel. In some embodiments, at least one port of the fluidic valve is connected to the central channel, and at least one port of the fluidic valve is connected to the downstream channel.
  • the opposite end of the active valve (distal end, furthest away from the fluidic channel) is connected to a pump.
  • the opposite ends of each active valve may be connected to a joint channel that is connected to a common pump.
  • the active valve when the active valve is closed, a pocket of air is trapped between the valve and any fluid in the inlet chamber of the cuvette, such that if fluid in the inlet chamber of the cuvette starts to move out of the inlet chamber under the influence of gravity, the air pocket between the inlet chamber and the valve becomes pressurized and therefore resists further movement of fluid from the cuvette.
  • the volume or pocket of air between the valve and any liquid in the cuvette inlet chamber has a volume ranging from 5 microliters to 60 microliters.
  • the cuvette module does not comprise an active valve.
  • the cuvette module comprises a pump.
  • the pump such as a peristaltic pump, membrane pump, or diaphragm pump, is configured so that it behaves as a closed fluidic valve when it is at rest, thereby enabling a volume of air to be trapped between the liquid in the cuvette and the pump.
  • FIG. 15G illustrates the state of the module before liquid has been dropped or dispensed into the cuvette. The cuvette, tube and upstream side of the pump are all at atmospheric pressure. FIG.
  • FIG. 15H illustrates an embodiment in which liquid has been dropped or dispensed into the cuvette, thereby trapping a volume of air, VI, between the pump and the liquid in the inlet chamber of the cuvette.
  • the pressure at the first capillary valve increases to a pressure equal and opposite to the head-pressure of the liquid.
  • FIG. 15H illustrates the situation where this equal-and-opposite pressure is generated exclusively by the capillary forces at the air/liquid interface.
  • the liquid has not entered the restrictive channel; rather the air/liquid interface itself creates the pressure.
  • FIG. 151 illustrates an alternative situation wherein some of the liquid in the cuvette begins to pass into the restrictive channel of the cuvette, thereby compressing the trapped volume of air to a volume V2.
  • the pressure of the trapped air again rises to be equal and opposite to the head pressure of the liquid.
  • the compression of the trapped air volume that generates the equal and opposite air pressure that resists further movement of the liquid out of the cuvette.
  • both capillary pressure and compressed-air pressure contribute to the pressure that resists the liquid head pressure.
  • the equilibrium position of the liquid/air meniscus may lie between the extremes shown in FIG. 15H and FIG. 151. In other words, the equilibrium position of the liquid/air meniscus may lie anywhere within the restrictive channel of the cuvette depending on the liquid height, the capillary forces associated with the first capillary valve, and the volume of air trapped between the liquid and the pump.
  • FIG. 15J illustrates an optional aspect of this embodiment in which the pump may be configured to apply a very slight pressure to the air-chamber, thereby momentarily increasing the pressure in the trapped air volume above the head-pressure of the liquid. In this way any fluid in the restrictive channel may be pushed back into the inlet chamber of the cuvette.
  • FIG. 15K illustrates a state in which the cuvette is to be emptied, and the pump pulls the fluid out of the cuvette.
  • the cuvette module comprises one or more filter holders into which filters may be inserted so that they are positioned proximal to the cuvettes, as shown in FIG. 16B.
  • filters may be high-, low-, or bandpass wavelength-selective filters, or they may be polarization or neutral density filters.
  • wavelength selective filters can be used to filter out wavelengths from a broad-spectrum light source, so that only an assay-appropriate band of wavelengths is detected by the detector.
  • the wavelength selective filters are configured to provide improved signal/noise during detection.
  • polarization filters may be used with polarization-based assays, wherein a first polarizer near the light-source creates linearly polarized light which passes through the cuvette and experiences an assay-dependent change in polarization. A second, crossed polarizer on the other side of the cuvette then only transmits light that has undergone the transition in polarization state.
  • neutral density filters can be used to attenuate light received by a detector if the light source is too bright and the light source itself cannot easily be adjusted.
  • the filters can be swapped in and out of the filter holders, enabling the cuvette module to accommodate a variety of assays.
  • the module comprises a housing that has holes in it to permit light from a light source to pass through the cuvette and any liquid contained therein, to a detector located on the opposite side of the cuvette.
  • the light source may be attached to a vertically-mounted PCB board, while the detectors may be arranged on a corresponding vertically-mounted PCB board.
  • the light-source and detector boards may be connected to a common motherboard, which may have its own power and a microcontroller.
  • the motherboard is capable of capturing data from the detector board and transmitting the data via a communication protocol (such as USB, Ethernet or RS-485) to a master microcontroller.
  • the discrete light-source and detector PCB boards may be easily swapped out by unplugging them from the motherboard, enabling the module to be rapidly reconfigured with different light sources and detectors to enable different photometric functions.
  • the cuvette module comprises a light source, or a plurality of light sources.
  • the cuvette module may be positioned adjacent to a light source, or plurality of light sources.
  • Each light source can be an optical emitter, such as a light-emitting-diode (LED) laser, or lamp, as shown in FIG. 13, FIG. 14, and FIG. 16 A.
  • the optical emitter can include an LED spacer and LED board as shown in FIG. 14 and 16A.
  • a broad-spectrum light source may be used with a filter wheel, prism, or grating to enable select wavelengths to be transmitted to particular cuvettes. Alternatively, a narrow band wavelength light source may be used.
  • the light from each light source is guided such that it is directed through the cuvette until it reaches the optical detector.
  • the detector may be a silicon photodiode, silicon photomultiplier, avalanche photodiode (APD), photomultiplier tube (PMT), or any other light detector such as are commonly used in the art,
  • the signal detected at the detector is acquired, amplified, and evaluated through means that are well-known to those versed in the art, so that the presence of an analyte and/or its concentration in the test fluid can be determined.
  • the absorbance of light A at wavelength caused by the presence of species X at a concentration [X] along a path L through the sample, can be expressed as: the millimolar absorptivity of the species X at the designated wavelength. Accordingly, the concentration of the sought-after species can be expressed as
  • Illumination beam paths in accordance with embodiments of the present disclosure may also include various light sources configured to generate light in the visible spectrum, having a wavelength ranging from 390 to 700 nm, or in the UV spectrum, having a wavelength ranging from 300 to 390 nm, or in the infrared spectrum having a wavelength ranging from 700-1500.
  • the light source may be a laser.
  • the light source may be a light-emitting diode (LED).
  • the light source is configured to emit light having a wavelength of, e.g., 340 nm, 365 nm, 405 nm, 488 nm, 514 nm, 525 nm, 561 nm, 594 nm, or 616 nm, 640 nm, or 660 nm, however, other wavelengths are possible.
  • each cuvette in an array of cuvettes of the cuvette module has a dedicated light source.
  • a single light source is shared by multiple cuvettes.
  • the light source and/or the cuvette module is motorized so that it may move by translation or rotation to positions that enable the light source to be delivered to multiple cuvettes.
  • FIG 25 shows an example of a motorized cuvette module that rotates each cuvette into position between a shared light source and detector.
  • more than one light source can be associated with a single cuvette.
  • several surface-mount LEDs may be positioned close to each other, such that light from all of the LEDs can be directed to a single cuvette.
  • the cuvette module comprises a sensor configured to detect the presence or absence of fluid in at least a portion of the cuvette.
  • the sensor is positioned around the neck of the cuvette in a location at which it can detect fluid in the outlet channel or restrictive channel of the neck.
  • the sensor is an optical sensor, capacitive sensor, or conductivity sensor.
  • the cuvette module comprises a pressure sensor configured to measure the pressure of the volume of air between the cuvette and the active valve.
  • the cuvette module comprises one or more fluidic sensors configured to determine the presence or absence of liquid in at least a portion of the cuvette.
  • the fluidic sensor is positioned around the necked region of the cuvette and can detect the position of the liquid/air meniscus in the restrictive channel therein. The location of the meniscus could be used as a check that the cuvette module is working as intended to maintain fluid in the inlet chamber of the cuvette.
  • this sensor is an optical sensor having an emitter and a detector, configured such that the emitter of the sensor is positioned on one side of the restrictive channel in the neck of the cuvette, and the detector on the other.
  • the sensor may alternatively be a capacitive sensor that can sense the change in the dielectric constant when air in the restrictive channel of the cuvette is replaced with liquid.
  • the pressure sensor will measure a pressure in the now-trapped volume of air between the cuvette and the pump that is equal to the head pressure of the liquid in the cuvette.
  • the pressure sensor may provide feedback about the height of the liquid in the cuvette inlet chamber.
  • the pump has been used to (optionally) provide a slight overpressure to the volume of trapped air, thereby ensuring that any liquid that has entered the restrictive channel is pushed back up into the cuvette inlet chamber.
  • the pressure sensor would sense the slight addition of pressure.
  • FIG. 15K during the evacuation of the cuvette, the pressure sensor will measure reduction in pressure associated with pulling the liquid out of the cuvette.
  • a characteristic pressure “signature” may be recorded and compared with the expected pressure signature. Significant deviation of the experimental signature from the expected signature may enable diagnostic assessment of hardware failure, clogs or unintended restrictions in the pump, valve, tubing, channels, or cuvette.
  • the cuvette is rinsed by the same pipettor used to conduct assays.
  • This requires no additional hardware, but is a serial process and occupies pipettor time, and often adds to the total analysis time (e.g., the total time a required in a run before another run can begin).
  • the cleaning fluid differs from the system fluid, it can become inefficient to use the pipettor as means to distribute the cleaning fluid, as it requires the pipette to prime with cleaning fluid, dispense the cleaning fluid, and then prime with system fluid before the next run, leading to the generation of more liquid waste and loss of time.
  • the cuvette module further comprises a fluid input system, wherein each cuvette has a dedicated tube or channel configured to convey fluid (such as a wash fluid) to the cuvette.
  • fluid such as a wash fluid
  • the cuvette may have an additional inlet hole, positioned such that it is above the expected fill level of the cuvette inlet chamber.
  • the cuvette module may have a tube or channel configured to interface with the inlet hole of the cuvette.
  • a fluid-tight gasket may be used to ensure a fluid-tight seal of the inlet tube with the cuvette.
  • wash fluid may be pumped through the fluid tube into the cuvette by an external pump (not shown) such as a peristaltic pump or membrane pump.
  • the cuvette does not comprise a inlet hole, and the inlet tube instead interfaces to the cuvette inlet.
  • the inlet tube may be preferentially located in a corner of the cuvette inlet so that it does not obstruct the remainder of the inlet.
  • the cuvette module comprises an optical mask that limits the spatial extent of the light source.
  • FIG. 16A illustrates an optical mask that is integral to the heatblock.
  • the mask compmrises a small hole that limits the spatial extent of the light emitted from the LED.
  • This spatial filtering can be used, for example, to limit the angular spread of light entering the cuvette so that, for example, no light rays hit the liquid/air meniscus representing the top of the liquid in the cuvette.
  • the mask is tailored to the cuvette geometry.
  • the mask with the small hole illustrated in FIG 16 A. will spatially limit the light passing into the cuvette to a cone of light that might match the angular dimensions of the trapezoidal cuvette of FIG. 8A-8C.
  • a mask with a slit will spatially limit the light to a flat-topped pyramid of light that is better suited to a square cuvette.
  • other masks and cuvette geometry pairs are possible.
  • the cuvette module optionally comprises a spatial mask that can be used to limit the area of light incident on the detectors from the light source through the cuvettes.
  • the cuvette module of the present disclosure includes an optical detector.
  • the optical detector may be a silicon photodiode, silicon photomultiplier, avalanche photodiode (APD), photomultiplier tube (PMT), or any other light detector such as are commonly known in the art.
  • aspects of the present disclosure further comprise a system for processing a sample and/or performing photometric measurements of a sample.
  • An example of such a system is shown in FIG. 20.
  • the system of the present disclosure includes one or more cuvette module(s) as described herein.
  • the system further comprises a pump.
  • a pump of the system is used to remove fluid through an outlet of one or more cuvettes of the cuvette module(s). For example, once an active fluidic valve is opened, the pump, once turned on, creates a pressure difference across the fluid, resulting in the expulsion of the liquid from an outlet of the cuvette with the open valve.
  • the pump causes fluid to be expelled from only a cuvette outlet with an open active valve. For example, if the cuvette module comprises 10 cuvettes, and only 5 of the 10 cuvettes comprises an open active valve, the pump can remove fluid from the 5 cuvettes with an open active valve, without disruption of the remaining 5 cuvettes with closed active valves.
  • the pump is a peristaltic pump, a membrane pump, or a diaphragm pump.
  • the pump comprises an evacuated gas cylinder, configured to create a pressure difference across the fluid in the cuvettes by pulling on their outlets.
  • the pump is a pneumatic pump.
  • the pump comprises a pressurized gas cylinder to create a pressure difference across the fluid in the cuvettes by applying pressure to their inlets.
  • the system further comprises a cleaning fluid tank.
  • the cleaning fluid tank may be fluidically connected to a pipettor so that the pipettor can dispense cleaning fluid into the cuvettes to clean them. This is the configuration shown in FIG.20 Alternatively, the cleaning fluid may be directly connected to the cuvettes through a valve, or through fluidic inlet tubes as shown in FIG 22.
  • the system further comprises a cleaning pump.
  • the cleaning pump can be used to pump cleaning fluid from the cleaning fluid tank, to the cuvettes.
  • the system further comprises a degasser.
  • the degasser can be used to degas fluids before they are used in the system.
  • the system further comprises one or more pipettor(s) for pipetting and/or mixing the sample and/or reagents in the cuvette(s).
  • the pipettor(s) are automated pipettor(s).
  • the system comprises a plurality of pipettors.
  • the pipettor(s) are mounted to arms on a robot, such as an XYZ gantry robot to enable them to move to different places in the system. An example of the pipettor configuration and orientation is provided in FIG. 24. 5.6.6. Thermal Heater
  • the system further comprises a thermal heater or a thermal block.
  • the thermal heater of the system can be used to maintain the temperature inside of an enclosed system generally, or inside the cuvette module, specifically.
  • the system further comprises one or more waste and wash stations.
  • the waste / wash station(s) can be used to accept waste from the pipettor(s) and to the inside and outside of the pipettors.
  • the system further comprises one or more consumable reagent plates.
  • the reagent plate(s) hold the reagents that are to be used in the assay(s). When the reagents are used up, the consumable reagent plate is replaced.
  • the system further comprises one or more sample vials.
  • the sample vial(s) hold the sample(s) that are to be used in the assay(s). After a sample is used, the sample vial is replaced.
  • the system further comprises a mixing apparatus.
  • a mixing apparatus includes magnetic stir bars or magnetic balls within the cuvette, and a magnet, such as a bar magnet may be positioned outside of the cuvettes. Movement of the bar magnet affects movement of the balls or bar magnets within the cuvettes, thereby creating turbulence that mixes the fluids.
  • Another non-limting example of a mixing apparatus includes mechanical features such as propellers or impellers that move into the cuvette to mix and retract from the cuvettes during measurement operations.
  • the system comprises mechanical means of mixing one or more fluids within the cuvette.
  • the system comprises non-mechanical means of mixing fluids within the cuvette. 5.7. Method for Processing a Sample
  • the cuvette module is a multiplexed device that can process and measure multiple samples in a single cuvette or in an array of cuvettes.
  • FIG. 19 describes a non-limiting example of use of the cuvette module.
  • step 1 the valve below the cuvette is closed. Liquids are pipetted into the cuvette and mixed, if necessary.
  • Step 2 of FIG. 19A the valve remains closed during the assay - a photometric assay is illustrated here.
  • step 3 of FIG. 19A the valve is opened - either by electrically actuating an active valve, and/or by creating a pressure differential across a passive valve (e.g. a check-valve, duckbill-valve, or capillary valve), and thereby causing it to open.
  • a passive valve e.g. a check-valve, duckbill-valve, or capillary valve
  • Such a pressure differential might be generated by using a pump to pressurize the top of the open cuvette, or pull a vacuum on the bottom of the cuvette on the distal side of the valve.
  • Other operating methods and modalities may also be envisioned.
  • the pipettor first aspirates Reagent 1 (Rl) from a well in the consumable cartridge and dispenses it to the cuvette on the cuvette module.
  • the pipettor washes itself in a wash station and then aspirates sample (e.g. such as plasma or serum) from the sample tube, dispenses it into the cuvette, and mixes it with Rl .
  • sample e.g. such as plasma or serum
  • the pipettor washes itself again in the wash station while Rl and sample are incubating.
  • the pipettor aspirates Reagent 2 (R2) from a second well in the consumable cartridge, dispenses it into the cuvette on the cuvette module and mixes.
  • the reaction now proceeds, and the intensity of the light from the LED(s) is monitored over time by the detector. These photometric measurements may be used to determine the concentration of the chemical species under test.
  • a waste pump (not shown in FIG. 19B) pulls the waste to a waste chamber.
  • the pipette then positions itself above the cuvette and flushes it with consecutive clean and other solutions, which are pumped through the pipette.
  • Other types of assays such as photometric endpoint assays, may be conducted in a similar manner.
  • a fluidic solution is a wash solution.
  • the method comprises dispensing fluids into an inlet of the cuvette of the present disclosure to deliver the fluid such as sample fluid, reagents, buffers, cleaning fluids, etc.
  • the method comprises mixing one or more reagents and a sample within a cuvette.
  • said mixing can be performed using a pipettor.
  • the method comprises measuring a sample while processing the sample and acquiring data representing the sample while processing the sample. In some embodiments, the method comprises measuring a processed sample and acquiring data representing the processed sample.
  • FIG. 19 An example of a sample processing and measuring workflow of the present methods is shown in FIG. 19 As shown in FIG. 19 the pipettor dispenses fluids (e.g., sample and/or reagent(s),.) into a cuvette to fill the cuvette. The pipettor can be used to mix the sample with reagents and/or buffer solutions (step 1). At any point during processing of the sample, or after processing the sample, the sample is measured and analyzed (step 2 of FIG. 19). Once measurements are performed, the fluidic valve is opened and the sample fluid is aspirated from the cuvette (step 3).
  • fluids e.g., sample and/or reagent(s),.
  • the method includes analyzing the data representing the sample. In some embodiments, analyzing comprises determining a concentration of the one or more analytes in the sample.
  • acquiring data representing said sample comprises acquiring fluorometric measurements of the sample. In some embodiments, acquiring data representing the sample comprises measuring the absorbance of the sample. In some embodiments, acquiring data representing the sample comprises measuring the concentration of the sample.
  • the sample comprises one or more metabolic analytes selected from: Blood urea nitrogen (BUN), carbon dioxide (CO2), Creatinine, Glucose, chloride, potassium, sodium, calcium, Hemoglobin, Albumin (ALB), Total protein (TP), Alkaline phosphatase (ALP), Alanine transaminase (ALT), Aspartate aminotransferase (AST), Total Bilirubin, Amylase, Gamma-glutamyl transferase, Lipase, Magnesium, Phosphorus, Direct Bilirubin, Triglycerides, total Cholesterol, high-density-lipoprotein (HDL), Ammonia, lactic acid (LAC), Fructose, Lactate dehydrogenase (LDH), uric acid, bile acids, Hbalc, and Creatine kinase.
  • BUN Blood urea nitrogen
  • CO2 carbon dioxide
  • Creatinine Glucose
  • chloride potassium
  • FIG. 21 Another non-limiting example of the method workflow is shown in FIG. 21.
  • the method includes aspirating reagent 1 (Rl) from a consumable well (step 1), and dispensing Rl into the cuvette module cuvette (step 2).
  • the next step includes washing/rinsing the pipettor (step 3).
  • the next step of the method includes aspirating the sample from a serum tube (step 4) and dispensing the sample containing serum into the cuvette module cuvette containing Rl (step 5).
  • the pipettor can be used to aspirate and dispense the sample and/or reagent to and from the cuvette, or from a well or tube containing Rl and/or sample and into the cuvette.
  • the pipettor can also be used to mix the reagent Rl and sample within the cuvette module cuvette (step 6). After mixing the sample with Rl in the cuvette module cuvette, the pipettor is washed and/or rinsed to remove residual sample and/or reagent (step 7). The next step is aspirating reagent 2 (R2) (step 8) from a consumable well (well #2) and dispensing R2 into the cuvette module cuvette (step 9) and mixing R2 into the cuvette containing Rl and sample (step 10). Again the pipettor is washed (step 11). The next step includes measuring the sample using the optical emitter. Light is transmitted through an interrogation region of the cuvette and detected by a detector on the opposite side of the cuvette wall.
  • the measurements may be taken at any time point, for example: during processing of the sample, during a reaction of an assay being performed, or after the reaction.
  • the detector measures the wavelength(s) of light transmitted over time and as the reaction proceeds (step 12). Once the assay is finished, the cuvette is drained, cleaned, and washed and the fluid exiting the cuvette then flows through a waste line into a waste container (step 13).
  • the fluid is a sample.
  • the fluid is a sample and a reagent.
  • the fluid is a sample and one or more reagents mixed together.
  • the fluid is a sample mixed with a buffer.
  • the reagent is a buffer.
  • the sample is a biological sample.
  • the sample is a chemical sample.
  • samples include various fluids such as bodily fluids (such as whole blood, serum, plasma, cerebrospinal fluid, urine, lymph fluids), and other fluids (such as, for example, cell culture suspensions, cell extracts, cell culture supernatants).
  • a sample may be suspended or dissolved in, for example, buffers, extractants, solvents, and the like. Additional examples of samples are provided by fluids deliberately created for the study of biological processes or discovery or screening of drug candidates. The latter include, but are not limited to, aqueous samples that contain bacteria, viruses, DNA, polypeptides, natural or recombinant proteins, metal ions, or drug candidates and th66ixturetures.
  • the cuvettes that have been described thus far have an interrogation region that is contained within the inlet chamber. It is a common feature of these embodiments that the interrogation region may be filled by a fluid entering the inlet, without substantial trapping of air. In other words, the interrogation region of the cuvette may be completely filled under the influence of gravity.
  • FIG. 23 A shows an embodiment in which the interrogation region is contained within the inlet chamber. This is the embodiment that we have previously described herein.
  • the inlet chamber is separated from the interrogation region.
  • the inlet chamber may be filled under the influence of gravity. However to fill the interrogation region, a pressure differential is created across the fluid to push or pull the fluid into the interrogation region.
  • the inputs to multiple modules may be clustered together and thus easily accessible by a pipettor or probe, while the interrogation regions may be located elsewhere, to save space on the instrument.
  • the inlet chamber may be filled under the influence of gravity.
  • Valve# 1 is opened, and fluid is pushed or pulled into the joining channel.
  • the fluid Upon reaching the interrogation region, the fluid then uniformly fills it under the influence of gravity, while air escapes through the air-channel.
  • the interrogation region is sized and shaped such that surface tension and capillary forces within it are negligible compared to the force of gravity.
  • Valve#l is closed, valve #2 is opened, and the pump pulls the liquid to waste through the waste channel.

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Abstract

Des aspects de la présente divulgation comprennent des cuvettes utilisées dans des dosages photométriques. Des aspects de la présente divulgation comprennent également un module de cuvette utilisant la cuvette pour effectuer des dosages photométriques. Des aspects de la présente divulgation comprennent en outre des systèmes et des procédés pour effectuer des dosages photométriques à l'aide de la cuvette.
PCT/US2024/028033 2023-05-05 2024-05-06 Conception de cuvette destinée à être utilisée dans un module de cuvette Pending WO2024233492A1 (fr)

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AU2024269943A AU2024269943A1 (en) 2023-05-05 2024-05-06 Cuvette design for use in a cuvette module

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JP4861042B2 (ja) * 2006-04-17 2012-01-25 株式会社日立ハイテクマニファクチャ＆サービス 分光光度計
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US10215687B2 (en) 2012-11-19 2019-02-26 The General Hospital Corporation Method and system for integrated mutliplexed photometry module
ES2784785T3 (es) * 2014-09-29 2020-09-30 Bd Kiestra Bv Aparato para la inspección óptica de pequeños volúmenes de muestra líquida y cubetas para el mismo

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