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WO2024233363A3 - Methods and systems for isolating nucleic acids - Google Patents

Methods and systems for isolating nucleic acids Download PDF

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Publication number
WO2024233363A3
WO2024233363A3 PCT/US2024/027770 US2024027770W WO2024233363A3 WO 2024233363 A3 WO2024233363 A3 WO 2024233363A3 US 2024027770 W US2024027770 W US 2024027770W WO 2024233363 A3 WO2024233363 A3 WO 2024233363A3
Authority
WO
WIPO (PCT)
Prior art keywords
systems
methods
nucleic acids
isolating nucleic
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/027770
Other languages
French (fr)
Other versions
WO2024233363A2 (en
Inventor
Lauren Nicole Mach QUIGLEY
Benjamin Mcnally
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Foundation Medicine Inc
Original Assignee
Foundation Medicine Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Foundation Medicine Inc filed Critical Foundation Medicine Inc
Publication of WO2024233363A2 publication Critical patent/WO2024233363A2/en
Publication of WO2024233363A3 publication Critical patent/WO2024233363A3/en
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Described herein is a method for isolating nucleic acid molecules. The method can comprise: removing proteins from a solution comprising nucleic acid molecules; adding a substrate and a binding reagent comprising an alcohol to the solution after removing the proteins from the solution to bind the nucleic acid molecules to the substrate; and separating the substrate bound to the nucleic acid molecules from a remainder of the solution.
PCT/US2024/027770 2023-05-05 2024-05-03 Methods and systems for isolating nucleic acids Pending WO2024233363A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363464484P 2023-05-05 2023-05-05
US63/464,484 2023-05-05

Publications (2)

Publication Number Publication Date
WO2024233363A2 WO2024233363A2 (en) 2024-11-14
WO2024233363A3 true WO2024233363A3 (en) 2024-12-19

Family

ID=93431011

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/027770 Pending WO2024233363A2 (en) 2023-05-05 2024-05-03 Methods and systems for isolating nucleic acids

Country Status (1)

Country Link
WO (1) WO2024233363A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001071732A2 (en) * 2000-03-24 2001-09-27 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
US20120171675A1 (en) * 2009-06-18 2012-07-05 Qiagen Gmbh Method for isolating nucleic acids
US20220403471A1 (en) * 2019-08-27 2022-12-22 Exact Sciences Corporation Characterizing methylated dna, rna, and proteins in subjects suspected of having lung neoplasia

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001071732A2 (en) * 2000-03-24 2001-09-27 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
US20120171675A1 (en) * 2009-06-18 2012-07-05 Qiagen Gmbh Method for isolating nucleic acids
US20220403471A1 (en) * 2019-08-27 2022-12-22 Exact Sciences Corporation Characterizing methylated dna, rna, and proteins in subjects suspected of having lung neoplasia

Also Published As

Publication number Publication date
WO2024233363A2 (en) 2024-11-14

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