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WO2024227050A3 - Engineered type iii rna-targeting crispr effectors - Google Patents

Engineered type iii rna-targeting crispr effectors Download PDF

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Publication number
WO2024227050A3
WO2024227050A3 PCT/US2024/026605 US2024026605W WO2024227050A3 WO 2024227050 A3 WO2024227050 A3 WO 2024227050A3 US 2024026605 W US2024026605 W US 2024026605W WO 2024227050 A3 WO2024227050 A3 WO 2024227050A3
Authority
WO
WIPO (PCT)
Prior art keywords
type iii
targeting crispr
engineered type
iii rna
crispr effectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/026605
Other languages
French (fr)
Other versions
WO2024227050A2 (en
Inventor
Daniel BROGAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of WO2024227050A2 publication Critical patent/WO2024227050A2/en
Publication of WO2024227050A3 publication Critical patent/WO2024227050A3/en
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

This disclosure provides a synthetic or engineered protein or polypeptide comprising a Cas7.1 peptide and a Casl or Casl 1 peptide, the synthetic or engineered protein or polypeptide having RNA-targeting function. This disclosure further provides CRISPR systems including the polypeptide or protein and methods of use of the CRISPR systems.
PCT/US2024/026605 2023-04-26 2024-04-26 Engineered type iii rna-targeting crispr effectors Pending WO2024227050A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363462195P 2023-04-26 2023-04-26
US63/462,195 2023-04-26

Publications (2)

Publication Number Publication Date
WO2024227050A2 WO2024227050A2 (en) 2024-10-31
WO2024227050A3 true WO2024227050A3 (en) 2025-03-13

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/026605 Pending WO2024227050A2 (en) 2023-04-26 2024-04-26 Engineered type iii rna-targeting crispr effectors

Country Status (1)

Country Link
WO (1) WO2024227050A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024227050A2 (en) * 2023-04-26 2024-10-31 The Regents Of The University Of California Engineered type iii rna-targeting crispr effectors

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220073891A1 (en) * 2020-09-02 2022-03-10 Massachusetts Institute Of Technology Systems, methods, and compositions for rna-guided rna-targeting crispr effectors
US20230383288A1 (en) * 2022-05-25 2023-11-30 Massachusetts Institute Of Technology Systems, methods, and compositions for rna-guided rna-targeting crispr effectors
WO2024227050A2 (en) * 2023-04-26 2024-10-31 The Regents Of The University Of California Engineered type iii rna-targeting crispr effectors

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220073891A1 (en) * 2020-09-02 2022-03-10 Massachusetts Institute Of Technology Systems, methods, and compositions for rna-guided rna-targeting crispr effectors
US20230383288A1 (en) * 2022-05-25 2023-11-30 Massachusetts Institute Of Technology Systems, methods, and compositions for rna-guided rna-targeting crispr effectors
WO2024227050A2 (en) * 2023-04-26 2024-10-31 The Regents Of The University Of California Engineered type iii rna-targeting crispr effectors

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KATO KAZUKI, ZHOU WENYUAN, OKAZAKI SAE, ISAYAMA YUKARI, NISHIZAWA TOMOHIRO, GOOTENBERG JONATHAN S., ABUDAYYEH OMAR O., NISHIMASU H: "Structure and engineering of the type III-E CRISPR-Cas7-11 effector complex", CELL, ELSEVIER, AMSTERDAM NL, vol. 185, no. 13, 27 May 2022 (2022-05-27), Amsterdam NL , pages 2324 - 2337.e16, XP093075387, ISSN: 0092-8674, DOI: 10.1016/j.cell.2022.05.003 *
ÖZCAN AHSEN; KRAJESKI ROHAN; IOANNIDI ELEONORA; LEE BRENNAN; GARDNER APOLONIA; MAKAROVA KIRA S.; KOONIN EUGENE V.; ABUDAYYEH OMAR : "Programmable RNA targeting with the single-protein CRISPR effector Cas7-11", NATURE, vol. 597, no. 7878, 6 September 2021 (2021-09-06), pages 720 - 725, XP037922752, DOI: 10.1038/s41586-021-03886-5 *
YU ET AL.: "Structure and function of a bacterial type III-E CRISPR- Cas 7-11complex", NATURE MICROBIOLOGY, vol. 7, 27 October 2022 (2022-10-27), pages 2078 - 2088, XP093075493, DOI: 10.1038/s41564-022- 01256-z *

Also Published As

Publication number Publication date
WO2024227050A2 (en) 2024-10-31

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