[go: up one dir, main page]

WO2024223764A1 - Bande pour libérer une composition antiparasitaire pour des abeilles pour lutter contre des infections parasitaires dans des ruches - Google Patents

Bande pour libérer une composition antiparasitaire pour des abeilles pour lutter contre des infections parasitaires dans des ruches Download PDF

Info

Publication number
WO2024223764A1
WO2024223764A1 PCT/EP2024/061440 EP2024061440W WO2024223764A1 WO 2024223764 A1 WO2024223764 A1 WO 2024223764A1 EP 2024061440 W EP2024061440 W EP 2024061440W WO 2024223764 A1 WO2024223764 A1 WO 2024223764A1
Authority
WO
WIPO (PCT)
Prior art keywords
strip
antiparasitic
composition
openings
weight
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2024/061440
Other languages
English (en)
Inventor
Ana AZNAR SANGUESA
Albert LLORCA SAGRERA
Xènia MERCADER I PUNTONET
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Laboratorios Calier S A
Original Assignee
Laboratorios Calier S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Laboratorios Calier S A filed Critical Laboratorios Calier S A
Publication of WO2024223764A1 publication Critical patent/WO2024223764A1/fr
Priority to MX2025012632A priority Critical patent/MX2025012632A/es
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K51/00—Appliances for treating beehives or parts thereof, e.g. for cleaning or disinfecting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A01N37/04—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof polybasic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/02—Acaricides

Definitions

  • Strip for releasing an antiparasitic composition to bees to control parasitic infections in beehives Strip for releasing an antiparasitic composition to bees to control parasitic infections in beehives.
  • the present disclosure refers to treatments for controlling parasites such as e.g., Varroa and Nosema in honeybees. More specifically, the present disclosure refers to a strip for releasing an antiparasitic composition to honeybees for controlling parasitic infections in beehives.
  • Varroa mites are one of said parasites that causes a disease called Varroasis which has been found that can destroy beehives completely.
  • Another type of parasite is Nosema, a type of spore forming fungus, responsible for nosemosis, which causes digestive disorders and may impair the ability of bees to fly.
  • CCD Colony Collapse Disorder
  • antiparasitic agents have been used to kill Varroa mites or Nosema spores, antiparasitic agents have shown to be toxic for bees and also to humans even when delivered in recommended dosages. Other antiparasitic agents are simply not as effective.
  • oxalic acid which has been found to be highly effective as acaricide against Varroa.
  • Known methods for applying oxalic acid to the beehives include dripping or sublimation. In the dripping method, the beekeepers have to prepare first a sugar solution containing a desired concentration of oxalic acid and then use it within 24 hours of its preparation. In the sublimation method, a special sublimation apparatus is needed.
  • antiparasitic compositions have also been applied on strips made of different materials which are intended to be placed between frames of beehives.
  • cellulose strips have been used to deliver formulations containing oxalic acid and glycerin.
  • plastic polymer strips with an active substance intended to be attached to beehives are known from W02020161155A1.
  • devices for releasing pesticidal vapors as the ones disclosed in W02015071890A1 and US2018116198A1 that comprise an absorbent substrate impregnated with a pesticidal material and an impermeable membrane. The substrate has dimples forming a waffled surface acting as wells to retain the pesticidal composition.
  • a strip for releasing a veterinary antiparasitic composition to honeybees in beehives to control parasites such as Varroa mites or Nosema spores is provided herein.
  • the present strip has been found to effectively address said need while providing further significant advantages as it will be described herein below.
  • the strip disclosed herein comprises a number of through openings that can be impregnated with an antiparasitic composition.
  • the special configuration of these through openings allows for good retention of a semi-solidified or solidified antiparasitic composition with specific characteristics adapted to the configuration of the strip.
  • the use of the strip impregnated with the antiparasitic composition results in that the antiparasitic active agent not only does not drip into the beehive in an uncontrolled manner but is only released by contact with the bees in the beehive.
  • these through openings also allow a uniform distribution of the composition over the whole surface of the strip which facilitates that the bees come into contact with the composition.
  • a veterinary antiparasitic composition for being released to honeybees through the present strip is also described herein.
  • the veterinary antiparasitic composition disclosed herein has been specially designed to have the adequate properties of consistency which allows its use in the strip herein disclosed.
  • the composition comprising an antiparasitic active agent, a wax, a fatty amide, and optionally a solvent has liquid consistency at a temperature above room temperature, e.g., at about 65 °C, and can be easily impregnated at this temperature onto the strip by filling the through openings provided for the reception of the composition.
  • the composition When the composition is cooled down to room temperature it solidifies in said through openings and remains adhered to them thanks to its semi-solid state consistency and the special structural configuration of the holes.
  • the combined development of the mechanical configuration of the strip and the specific characteristics of the antiparasitic composition allows providing a ready to use product which shows very good efficacy against parasites, has reduced toxicity for bees, is stable during production, storage, distribution, and manipulation, and is safe for beekeepers.
  • the strip is configured to be inserted between beehive frames to remain inside the beehives for a certain period of time as it will be described below.
  • the strip has a first surface to be impregnated with the antiparasitic composition to be released to bees in a beehive.
  • Said first surface has at least a first area with a number of through openings.
  • the through openings are intended for receiving the antiparasitic composition therein. At least some of said through openings has an interior that defines a first portion and a second portion and an intermediate portion between and narrower than the first portion and the second portion.
  • a cross-section of the interior of the through openings may include walls sloped at an angle to each other such as for example of 20-30°. Said walls defining the crosssection of the interior of the through openings may be for example straight or curved or a combination of both, although many other configurations are possible.
  • the through openings formed in the first surface may be shaped in the form of two inverted cones. It is preferred that the through openings are defined by a hexagonal perimeter, that is, through openings are preferably hexagonal in shape when viewed from the top or the bottom. Hexagonal shape resembles natural six-sided honeycomb structures allowing to compact a large number of through openings.
  • An interior of at least some of said through openings may be configured such that the second portion is wider than the first portion.
  • the substance to be released can be held suitably inside the through openings and thus applied to both surfaces of the strip.
  • the second portion may be preferably covered by a peelable sheet.
  • the peelable sheet is glued to a second surface of the strip, opposite the first surface.
  • adhesive may be applied to the second surface of the strip. Taking into account that the mutually opposite first and second surfaces of the strip have a similar configuration, the adhesive may be applied in particular to the first area with through openings in the second surface of the strip. In general, adhesive may be applied to at least one of the first surface and the second surface of the strip.
  • the peelable sheet prevents the antiparasitic composition from leaking out from the through openings as it is applied hot, allowing the antiparasitic composition to cool down until it solidifies within the through openings. Once the antiparasitic composition has been solidified, the peelable sheet is removed before placing the strip inside the beehive causing the antiparasitic composition, already solidified, to be available on both surfaces of the strip.
  • the substance could be alternatively applied to only one surface of the strip if required.
  • the first surface of the strip may have a second area.
  • a thickness of the first area of the first surface is preferably thicker than a thickness of the second area of the first surface.
  • the second area of the first surface of the strip includes two or more spaced apart sets of weakening lines. Weakening lines allow portions of the second area of the first surface of the strip to be folded to define a hanging arrangement.
  • the hanging arrangement is suitable for suspending the strip at different heights in a beehive frame.
  • the hanging arrangement is sized such that the strip may be hung in frames of the most commonly used beehives, namely Langstroth and Dadant hives.
  • a veterinary antiparasitic composition to be released into the beehive through the above-described strip is also disclosed herein.
  • Said veterinary antiparasitic composition comprises: a) a therapeutically effective amount of an antiparasitic active agent; b) a wax; c) a fatty amide of formula (I)
  • R 1 is a substituted or unsubstituted (C8-Cso)hydrocarbon group
  • R 2 is selected from hydrogen, an hydroxyl group, and a substituted or unsubstituted (C8-Cso)hydrocarbon
  • R 3 is selected from hydrogen, a substituted or unsubstituted (C8-Cso)hydrocarbon, and -(C8-Cso)-NHC(0)-R 4 , wherein R 4 is a substituted or unsubstituted (C8-C3o)hydrocarbon group.
  • d) optionally a solvent; and optionally one or more further veterinary acceptable excipients.
  • a further aspect of the disclosure refers to a ready to use product which comprises the strip provided with a number of through openings as defined herein, the strip being impregnated with the antiparasitic composition.
  • Figure 1 is a top plan view of one example of a strip for releasing an antiparasitic composition to bees to control Varroa in beehives;
  • Figure 2 is a cross-sectional view of the strip in figure 1 ; and Figure 3 is a detail cross-sectional view of one opening in a first area of the strip in figures 1 and 2.
  • any ranges given include both the lower and the upper endpoints of the range.
  • terapéuticaally effective amount refers to the amount of antiparasitic active agent which, when administered to a beehive, is sufficient to kill, inhibit, reduce, slow, stabilize or control the growth of a mite population.
  • the specific dose of the antiparasitic active agent to obtain a therapeutic benefit may vary depending on the particular circumstances of the individual case including, among others, the type of beehive and the nature and stage of a parasitic infection.
  • veterinary acceptable excipients refers to components which are appropriate for use in veterinary technology for the preparation of compositions for veterinary use. Each component should be “acceptable” in the sense of being compatible with the other ingredients of the composition. When used in contact with the bees should not have excessive toxicity or other related problems or complications such as contamination of wax or honey in the beehive, commensurate with a reasonable benefit/risk ratio.
  • antiparasitic refers to an active agent or composition which is able to kill the mites, bacteria or fungal spores, or is able to inhibit, reduce, slow, stabilize or control the growth of a parasite population.
  • antiparasitic comprises the terms “miticide”, “miticidal”, which are hereby equivalent to the terms “acaricide” or “acaricidal”, respectively.
  • parasites include “mites”, “acarids”, “bacteria” or “fungus”.
  • parasitic infection refers to the colonization or parasitisation of a beehive by mites, bacteria or fungal spores.
  • treatment of a parasitic infection means reducing, stabilizing, inhibiting, controlling or slowing the growth of a parasite population in the beehive.
  • prevention of a parasitic infection means avoiding that a parasitic infection is established in a beehive.
  • ready to use product refers to a finished product comprising a strip impregnated with the veterinary antiparasitic composition specially designed for the strip which allows its prompt and direct use without require any additional preparation or assembling steps to be performed by the user (beekeeper) before use.
  • impregnated means that the antiparasitic composition as defined herein is held within the through openings present in the strip.
  • hydrocarbon refers to a linear or branched, saturated or unsaturated hydrocarbon having a number of carbon atoms as specified herein.
  • room temperature means a temperature from 20 to 25 °C.
  • a strip 10 for releasing an antiparasitic composition to honeybees to control Varroa in beehives is provided.
  • Each strip 10 has a first surface A and a second, opposite surface B as shown in figure 2 of the drawings.
  • the first surface A of the strip 10 has at least a first area 20 where a number of through hexagonal openings 21 are formed for receiving therein the antiparasitic composition.
  • a suitable number of strips 10 may be provided to a beehive, arranged between beehive frames. The strips 10 are left for a certain period of time during which the antiparasitic composition is uniformly released, impregnating the bees by contact, distributing it throughout the beehive.
  • the cross-section of the interior of the through openings 21 comprises straight or curved walls 21a, 21b sloped at an angle to each other of 20-30° with 24° being preferred.
  • the walls 21a, 21b thus define two inverted cones, that is, a cross-section in the shape of a double trapeze.
  • the antiparasitic composition impregnated to the strip 10 is a viscous a liquid above 60 - 65° C and solidifies into a dense paste below that temperature range. Other temperature ranges may be involved depending on the composition used for impregnating the strip 10.
  • the antiparasitic composition is delivered hot through dosing nozzles into the through openings 21.
  • the inclined surfaces 21a, 21b defined in the interior of the through openings 21 when the antiparasitic composition in liquid form is hot poured into the first portion 22 of the through openings 21 of the strip 10 the antiparasitic composition is retained in the intermediate portion 24 as a result of which quick solidification is promoted.
  • the strip 10 is presented with a peelable sheet 25 that is glued to a second surface B of the strip 10, opposite the first surface B, through an adhesive 40.
  • the adhesive 40 is applied to the second surface of the strip B as shown in figure 1, and in particular in a first area 20 with through openings 21 of the second surface B of the strip.
  • the peelable sheet 25 By having the peelable sheet 25 glued to the second surface B of the strip 10 before the strip 10 is placed in the beehive frames, the second portion 23 of the through openings 21 is closed such that the antiparasitic composition that is delivered hot to the through openings 21 is held in their interior while it cools down until it solidifies.
  • the peelable sheet 25 thus prevents the antiparasitic composition from leaking out when being hot poured into the through openings 21 of the strip 10, as stated above.
  • the peelable sheet 25 Upon solidification of the formulation and prior to the application of the strip 10 inside the beehive, the peelable sheet 25 is removed causing the antiparasitic composition to be available in both surfaces A, B of the strip 10.
  • the first surface A of the strip 10 has a second area 30, as illustrated in figure 1 .
  • the second area 30 includes two spaced apart sets of weakening portions such as weakening lines 31 , 32.
  • the weakening portions may be weakening holes or other suitable strip areas formed in the second area 30 This results in portions of the second area 30 of the first surface A of the strip 10 being allowed to be folded, e.g. bent up to 90° to the strip 10, to define a hanging arrangement 35.
  • the hanging arrangement 35 allows the strip 10 to be suspended at different heights in a beehive frame preventing the strip from falling down the beehive frame.
  • the hanging arrangement 35 is configured to fit to frames for Langstroth and Dadant hives, as well as many other hives.
  • both mutually opposite first and second surfaces A, B of the strip 10 have a similar configuration as depicted in figure 1 of the drawings.
  • a step portion 50 is formed in the second surface B of the strip 10 resulting from the thickness 620 of the first area 20 of the first surface A being thicker than the thickness e 3 o of the second area 30 of the first surface A as illustrated in figure 2.
  • the strip 10 is sized to fit, for example, to frames of Langstroth and Dadant hives, as well as many other types of hives. Frames are usually 24 cm high, 51 .5 cm long, and 43 cm wide in Langstroth hives while frames are usually 29.6 cm high, 51.5 cm long, and 43 cm wide in Dadant hives.
  • the strip 10 is sized to reach the bottom of a beehive. Strip thickness 620, e 3 o is selected so that the strip 10 is allowed to be easily inserted between beehive frames without hampering the passage of the bees.
  • the weight of the strip 10 is 25-29 g.
  • the strip 10 is 310 cm long and 60 cm wide.
  • the first area 20 of the strip 10 is 225 cm long and the second area 30 of the strip 10 is 85 cm long.
  • the thickness 620 of the first area 20 of the strip 10 is 3.5 cm, and the thickness e 3 o of the second area 30 of the strip 10 is 1.2 cm.
  • Two parallel opposite sides of the hexagonal perimeter of the through openings 21 are spaced apart 6.9 cm in the first portion 22 for the antiparasitic composition in the interior of the through openings 21 .
  • two parallel opposite sides of the hexagonal perimeter of the through openings 21 are spaced apart 8 cm.
  • two parallel opposite sides of the hexagonal perimeter of the through openings 21 are spaced apart 6.2-7.1 cm. This results in a volume of the interior of the through openings 21 of 27 cm 3 .
  • Other values are possible for the present strip 10.
  • a veterinary antiparasitic composition suitable for use in the strip 10 as defined herein, which comprises an antiparasitic active agent, a wax, a fatty amide of formula (I), optionally a solvent, and optionally one or more further veterinary acceptable excipients.
  • Non-limiting examples of antiparasitic active agents that may be included in the antiparasitic composition include pyrethroids, amidine or related compounds such as abamectin (CAS RN 71751-41-2), acequinocyl (CAS RN 57960-19-7), acrinathrin (CAS RN 101007-06-1), amitraz (CAS RN 33089-61-1), bifenthrin (CAS RN 99267- 18-2), bromopropylate (CAS RN18181-80-1), chlorfenapyr (CAS RN 122453-73-0), clofentezine (CAS RN 74115-24-5), coumaphos (CAS RN 56-72-4), cyflumetofen (CAS RN 400882-07-7), cymiazole (CAS RN 61676-87-7), etoxazole (CAS RN 153233-91-1), fenpropathrin (CAS RN 39515-41-8), f
  • the antiparasitic active agent is selected from the group consisting of organic acids, pyrethroids, essential oils, and combinations thereof. More particularly, the antiparasitic active agent is selected from the group consisting of oxalic acid, fenazaquin, fenpyroximate, fluvalinate, and thymol, and even more particularly the antiparasitic active agent is oxalic acid, which can be in the form of oxalic acid dihydrate.
  • the antiparasitic active agent has low or no insecticidal activity, i.e. , has low or no toxicity against honeybees.
  • the bee mortality in a beehive that has been treated with the antiparasitic composition is the same than the bee mortality in a beehive that has not been treated with the antiparasitic composition.
  • the antiparasitic active agent is present in the composition in an amount from 1 to 50%, more particularly from 2 to 45%, even more particularly from 5 to 40%, and even more particular from 20 to 35% by weight with respect to the total weight of the composition. In another embodiment, optionally in combination with one or more features of the various embodiments described above or below, the antiparasitic active agent is present in the composition in an amount about 1%, about 2%, about 3%, about 4%, about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, or about 50% by weight with respect to the total weight of the composition.
  • the antiparasitic active agent is an organic acid, particularly oxalic acid, and is present in the composition in an amount from 4 to 35%, more particularly from 20 to 30%, by weight with respect to the total weight of the composition.
  • the antiparasitic active agent is a pyrethroid, more particularly fenazaquin or fenpyroximate, and is present in the composition in an amount from 1 to 10%, more particularly from 1 to 5%, by weight with respect to the total weight of the composition.
  • the antiparasitic active agent is an essential oil, more particularly thymol, and is present in the composition in an amount from 4 to 35%, more particularly from 20 to 30%, by weight with respect to the total weight of the composition.
  • the veterinary antiparasitic composition further comprises a wax.
  • waxes which can be used in the context of the present invention include waxes of mineral or vegetable origin, and synthetic waxes, which also include chemically modified natural waxes.
  • the wax has a viscosity from 25 to 80 mPas, more particularly from 30 to 50 mPa s, at 20 °C.
  • the wax is a (Cs-Csojhydrocarbon wax.
  • (Cs-Csojhydrocarbon wax” as used herein refers to a linear or branched saturated or unsaturated hydrocarbon of natural or artificial origin, which is composed solely of carbon and of hydrogen, and which contains from 8 to 50 carbon atoms.
  • the (Cs-Csojhydrocarbon wax is a saturated hydrocarbon.
  • the (Cs-Csojhydrocarbon wax is selected from the group consisting of petrolatum, paraffin, ozocerite, and ceresin. More particularly, the (Cs-Csojhydrocarbon wax is paraffin, even more particularly liquid paraffin (CAS RN: 8042-47-5).
  • the wax is a vegetable wax, more particularly selected from the groups consisting of carnauba wax and jojoba wax.
  • the wax is present in the composition in an amount from 3 to 55%, more particularly from 5 to 40%, even more particularly from 10 to 35%, and even more particular from 10 to 20% by weight with respect to the total weight of the composition.
  • the wax is present in the composition in an amount about 3%, about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 50%, or about 55%, by weight with respect to the total weight of the composition.
  • the veterinary antiparasitic composition further comprises a fatty amide of formula (I) R 1 -C(O)NR 2 R 3
  • R 1 is a substituted or unsubstituted (Cs-Csojhydrocarbon group
  • R 2 is selected from hydrogen, an hydroxyl group, and a substituted or unsubstituted (C8-Cso)hydrocarbon
  • R 3 is selected from hydrogen, a substituted or unsubstituted (C8-Cso)hydrocarbon, and -(C8-Cso)-NHC(0)-R 4 , wherein R 4 is a substituted or unsubstituted (C8-C3o)hydrocarbon group.
  • R 1 is substituted or unsubstituted (C8-Cso)hydrocarbon; R 2 is hydrogen or an hydroxyl group; and R 3 is hydrogen. More particularly, in the fatty amide of formula (I) R 1 is an unsubstituted unsaturated (C8-Cso)hydrocarbon; and R 2 and R 3 are hydrogen.
  • the fatty amide of formula (I) is selected from the group consisting of stearamide (CAS RN: 8042-47-5), oleamide (CAS RN 301-02-0), erucamide (CAS RN 112-84-5), behenamide (CAS RN 3061-75- 4), lauramide (CAS RN 1120-16-7), tridecanamide (CAS RN 34778-57-9), eicosanamide (CAS RN 51360-63-5), N-hydroxyoleamide (CAS RN 10335-69-0), palmitoleamide (CAS RN 106010-22-4), and a combination thereof. More particularly, the fatty amide of formula (I) is erucamide.
  • the fatty amide of formula (I) is present in the composition in an amount from 10 to 60%, more particularly from 20 to 55%, even more particularly from 25 to 45%, and even more particular about 30 to 35% by weight with respect to the total weight of the composition.
  • the wax is present in the composition in an amount about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, or about 60% by weight with respect to the total weight of the composition.
  • the weight ratio of the fatty amide of formula (I) and the wax is from 1 :4 to 6:1 , more particularly from 1 :1 to 6:1 , and even more particularly from 2:1 to 4:1.
  • weight ratio of the fatty amide of formula (I) and the wax is about 1 :4, about 1 :3.5, about 1 :3, about 1 :2.5, about 1 :2, about 1 :1.5, about 1 :1 , about 1.5:1 , about 2:1 , about 2.5:1 , about 3:1 , about 3.5:1, about 4:1 , about 4.5:1 , about 5:1 , about 5.5:1 , or about 6:1.
  • the veterinary antiparasitic composition may further comprise a solvent.
  • the veterinary antiparasitic composition comprises a solvent. More particularly, the solvent is a polyol, a vegetable oil, or a mixture thereof.
  • the veterinary antiparasitic composition comprises a solvent which is a polyol, more particularly a polyol selected from the group consisting of glycerin, propylene glycol, 1,2-butanediol, 1,3- butanediol, 1,2-pentanediol, 1,5-pentanediol, 1,2-hexanediol, 1,6-hexanediol, diglycerin, dipropylene glycol, 1,2,3-hexanetriol, 1,2,6-hexanetriol, and mixtures thereof.
  • a solvent which is a polyol, more particularly a polyol selected from the group consisting of glycerin, propylene glycol, 1,2-butanediol, 1,3- butanediol, 1,2-pentanediol, 1,5-pentanediol, 1,2-hexanediol, 1,6-hexanediol
  • the polyethylene glycol used in the antiparasitic composition has an average molecular weight (Mw) from 200 to 100000 g/mol.
  • Mw average molecular weight
  • polyethylene glycol having an average molecular weight from 200 to 8000 g/mol, more particularly from 200 to 1000 g/mol, and even more particularly from 200 to 400 g/mol may be used.
  • the veterinary antiparasitic composition comprises a solvent which is polyethylene glycol.
  • the veterinary antiparasitic composition comprises a solvent which is glycerin.
  • the veterinary antiparasitic composition comprises a solvent which is a vegetable oil, more particularly a vegetable oil selected from the group consisting of sunflower oil, olive oil, cardamom oil, palm oil, and mixtures thereof.
  • the veterinary antiparasitic composition comprises a solvent which is a mixture of a polyol and a vegetable oil, more particularly a mixture of a polyol selected from the group consisting of glycerin, propylene glycol, 1,2-butanediol, 1,3-butanediol, 1,2-pentanediol, 1,5-pentanediol, 1 ,2-hexanediol, 1,6-hexanediol, diglycerin, dipropylene glycol, 1 ,2,3-hexanetriol, 1 ,2,6-hexanetriol, and mixtures thereof; and a vegetable oil selected from the group consisting of sunflower oil, olive oil, cardamom oil, palm oil, and mixtures thereof; even more particularly a mixture of glycerin and sunflower oil.
  • a polyol selected from the group consisting of glycerin, propylene glycol, 1,2-butanediol, 1,
  • the veterinary antiparasitic composition comprises a solvent which is present in the composition in an amount from 20 to 50%, more particularly from 22 to 40%, and even more particularly from 25 to 35% by weight with respect to the total weight of the composition.
  • the wax is present in the composition in an amount about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50% by weight with respect to the total weight of the composition.
  • the veterinary antiparasitic composition does not comprise a solvent.
  • the antiparasitic composition as defined herein may also comprise one or more further veterinary acceptable excipients.
  • excipients include, without limitation, viscosity modifiers, surfactants, isotonic modifiers, emulsifiers, binders, preservatives, and the like.
  • the veterinary antiparasitic composition suitable for use in the strip 10 as defined herein comprises: a) a therapeutically effective amount of an antiparasitic active agent; b) from 3 to 55% by weight of a wax; c) from 10 to 60% by weight of a fatty amide of formula (I), as defined herein; d) optionally a solvent; and optionally one or more further veterinary acceptable excipients; wherein the percentages are given with respect to the total weight of the composition, and wherein the weight ratio of the fatty amide of formula (I) and the wax is from 1 :1 to 6:1.
  • the veterinary antiparasitic composition suitable for use in the strip 10 as defined herein comprises: a) from 1 to 50% by weight of an antiparasitic active agent; b) from 3 to 55% by weight of a wax; c) from 10 to 60% by weight of a fatty amide of formula (I) as defined herein; d) optionally a solvent; and optionally one or more further veterinary acceptable excipients; wherein the weight percentages are expressed with respect to the total composition weight, and being the sum of all the components of the composition of 100% by weight.
  • the weight ratio of the fatty amide of formula (I) and the wax is from 1 :4 to 6: 1.
  • the veterinary antiparasitic composition suitable for use in the strip 10 as defined herein comprises: a) from 1 to 50% by weight of an antiparasitic active agent; b) from 3 to 55% by weight of a wax; c) from 10 to 60% by weight of a fatty amide of formula (I) as defined herein; d) from 20 to 50% by weight of a solvent; and optionally one or more further veterinary acceptable excipients; wherein the weight percentages are expressed with respect to the total composition weight, and being the sum of all the components of the composition of 100% by weight.
  • the weight ratio of the fatty amide of formula (I) and the wax is from 1 :4 to 6: 1.
  • the veterinary antiparasitic composition suitable for use in the strip 10 as defined herein comprises: a) from 4 to 35% by weight of an organic acid, more particularly oxalic acid, even more particularly oxalic acid dihydrate; b) from 10 to 35% by weight of a wax, more particularly liquid paraffin; c) from 20 to 55% by weight of a fatty amide of formula (I) as defined herein, more particularly erucamide; d) from 25 to 35% by weight of a solvent, more particularly a polyol, even more particularly glycerin or polyethylene glycol; and optionally one or more further veterinary acceptable excipients; wherein the weight percentages are expressed with respect to the total composition weight, and being the sum of all the components of the composition of 100% by weight.
  • the weight ratio of the fatty amide of formula (I) and the wax is from 1:1 to 6: 1.
  • the veterinary antiparasitic composition suitable for use in the strip 10 as defined herein comprises: a) from 1 to 10% by weight of a pyrethroid, more particularly fenazaquin or fen pyroxi mate; b) from 10 to 35% by weight of a wax, more particularly liquid paraffin; c) from 30 to 60% by weight of a fatty amide of formula (I) as defined herein, more particularly erucamide; d) from 25 to 35% by weight of a solvent, a mixture of a polyol and a vegetable oil, even more particularly, a mixture of glycerin and sunflower oil; and optionally one or more further veterinary acceptable excipients; wherein the weight percentages are expressed with respect to the total composition weight, and being the sum of all the components of the composition of 100% by weight.
  • the weight ratio of the fatty amide of formula (I) and the wax is from 1:1 to 6: 1.
  • the veterinary antiparasitic composition suitable for use in the strip 10 as defined herein comprises: a) from 4 to 35% by weight of an essential oil, more particularly thymol; b) from 5 to 20% by weight of a wax, more particularly liquid paraffin; c) from 20 to 55% by weight of a fatty amide of formula (I) as defined herein, more particularly erucamide; d) from 25 to 35% by weight of a solvent, more particularly a mixture of a polyol and a vegetable oil, even more particularly, a mixture of glycerin and sunflower oil; and optionally one or more further veterinary acceptable excipients; wherein the weight percentages are expressed with respect to the total composition weight, and being the sum of all the components of the composition of 100% by weight.
  • the weight ratio of the fatty amide of formula (I) and the wax is from 1:1 to 6: 1.
  • a process for the preparation of the antiparasitic composition as defined herein comprising a solvent comprises: i) heating the solvent or part of the solvent at a temperature from 40 to 70 °C, particularly at about 50 °C, ii) adding the antiparasitic active agent to the solvent of step i), particularly under stirring, while keeping the temperature from 40 to 70 °C; iii) optionally adding the remaining part of the solvent; iv) adding the wax to the mixture of step ii) or iii), particularly under stirring, and heating to a temperature from 60 to 80 °C; and v) adding the fatty amide of formula (I) to the mixture of step iii), particularly until the fatty amide of formula (I) is dissolved.
  • a process for the preparation of the antiparasitic composition as defined herein comprising a solvent, wherein the process comprises: i’) heating the solvent at a temperature from 40 to 70 °C, particular at about 50 °C; ii’) adding the antiparasitic active agent to the solvent of step i’), particularly under stirring, while keeping the temperature from 40 to 57 °C; iii’) heating the wax, particularly under stirring, at a temperature from 60 to 80 °C; iv’) adding a fatty amide of formula (I) to the wax of step iii’), particularly until the fatty amide of formula (I) is dissolved; and v’) mixing the mixture of step ii’) with the mixture of step iv’).
  • a process for the preparation of the antiparasitic composition as defined herein in the absence of a solvent comprising: i”) heating the wax at a temperature from 80 to 85 °C; ii”) adding a fatty amide of formula (I) to the wax of step i’), particularly until the fatty amide of formula (I) is dissolved; iii”) adding the antiparasitic active agent to the step ii’), particularly under stirring, while keeping the temperature from 95 to 105 °C until the active agent is dissolved;
  • a ready to use product which comprises the strip 10 provided with a number of through openings as defined above and the antiparasitic composition impregnated onto said strip 10 is described below.
  • the ready to use product comprises from 5 to 60 g, more particularly from 20 to 35 g, of antiparasitic composition impregnated onto the strip 10.
  • oxalic acid dihydrate (Punjab Chemicals), thymol (Sigma-Aldrich), fenazaquin (Crysdot), fenpyroximate (Punjab Chemicals), glycerin (IOI Oleochemical), liquid paraffin (Dilube, CAS RN: 8042-47-5), erucamide (Equus UK Topco Ltd (Cargill)), Polyethylenglycol 300 (PEG300, Ineos Oxide), Polyethylenglycol 6000F (PEG6000PF, Clariant), Polyethylenglycol 8000F (PEG8000PF, Clariant), Carnauba Wax T3 flakes (Pontes Industrias de Cera), Beeswax (Sigma Aldrich), Crodabase SQ-SS-(BR)Croda/Croda), sunflower oil (Gustav Heess).
  • Glycerin or Polyethylenglycol 300 were introduced in a reactor and was heated to 50 °C.
  • Oxalic acid dihydrate was added to the reactor and the mixture was stirred for 20 min using propeller type agitation at a temperature 50 - 60 °C.
  • Glycerin or Polyethylenglycol 300 were introduced in a vessel and was heated to 50 °C.
  • Oxalic acid was added to the reactor and the mixture was stirred for 20 min using propeller type agitation at a temperature 50 - 60 °C.
  • Sunflower oil was introduced in a reactor and was heated to 50 °C (for thymol), 60 °C (for fenazaquin) and 70 °C (for fenpyroximate)
  • Thymol or fenazaquin or fenpyroximate was added to the reactor and the mixture was stirred for 20 min using propeller type agitation at a temperature 50 - 60 - 70 °C.
  • Paraffin wax was introduced into the reactor and heated until 80 - 85 °C.
  • Erucamide was added to paraffin and the mixture was heating under stirring at 80 - 90 °C. The solution was allowed to cool to 85 °C and oxalic acid was added under stirring maintaining the temperature at 70 - 85 °C. After 15 minutes the oxalic acid was not completely dissolved.
  • Erucamide was heated until completely melted at 80 °C. Oxalic acid was added but did not dissolve; an agglomerate has formed. Paraffin was added under stirring. The mixture was heated to 80 °C under stirring but oxalic acid did not dissolve.
  • Paraffin was heated to 80 °C. Erucamide was added little by little under magnetic stirring until erucamide was dissolved. Erucamide melted at 77 °C after 15 min. Oxalic acid was added. In order for the oxalic acid to dissolve, the mixture was heated to 100 °C. A pinkish product was obtained that gave off a pungent odor.
  • Glycerin was heated under stirring. Erucamide was added melting about 78 - 80 °C after 15 min. At 83 °C oxalic acid was incorporated and the temperature dropped to 77 °C. A wax was not formed.
  • PEG 300 was heated up to 60 °C and oxalic acid was added under stirring. Erucamide was added at 80 °C little by little and the mixture was stirred for 10 min. Crystallizations appeared.
  • Paraffin was heated to 60 °C.
  • PEG8000 was added and heated until dissolution.
  • the mixture was heated up to 90 °C but a phase separation is observed.
  • Oxalic acid was added under stirring until dissolution.
  • the mixture was heated up to 90°C but presented phase separation and air bubbles.
  • PEG 6000 was heated up to 60°C and melted. Paraffin was added under stirring. Oxalic acid was added until dissolution. However, paraffin and PEG 6000 remained immiscible.
  • strips with through openings (holes) according to the disclosure were used.
  • the through openings of the strip were filled with the formulation of the invention directly after the preparation of the formulation (i.e. , when the formulation had still liquid consistency, at about 65 °C).
  • Mechanised dispensers (as many as through openings in the strip) were used for this purpose, which allowed maintaining the product at the correct temperature, and allowed dosing the correct amounts to each opening.
  • a volume of the formulation was transferred to a buffer tank where the temperature was maintained to keep the formulation in liquid state.
  • the buffer tank supplied the formulation to a dose dispenser located in front of the buffer tank.
  • the temperature was maintained under control also in all the connections used to contain or transport the formulation.
  • An empty strip was placed below the dose dispenser. The strip was moved to the correct position and the exact quantity of formulation was dosed in any opening. This procedure was repeated for a new empty strip until all the formulation from the buffer tank was transferred to the strips.
  • the formulation After depositing the formulation to the through openings of the strip, the formulation was left to cool and at room temperature, the composition exhibited a semi-solid state, consistent enough to stay into the through openings on the strip. A label was sticked on the back to prevent leaking during filling and cooling down of the product.
  • EMA European Medicine Agency
  • EMA/CVMP/EWP/459883/2008-Rev.1* One study was with 2 strips placed in the hives for 6 weeks and the other was with 2 strips placed in the hives for 3 weeks, and after this, two strips were removed, and 2 new strips were placed in the hives for 3 more weeks. Trays for appropriate counting of the mites killed by treatment were used.
  • the rate of mite mortality after treatment with the product under investigation was determined using a follow-up treatment in the treated colony itself with a chemically unrelated substance with >95% documented efficacy.
  • the follow-up treatment was carried out shortly after treatment with the test product in order to keep the reinfestation level low.
  • the follow-up treatment was Apitraz (Calier) with a proven efficacy of about 99%.
  • the formulations of the invention showed very high efficacy values.
  • analog placebo formulations without antiparasitic agent were also tested in 15 hives. After 6 weeks, it was observed that the efficacy for these placebo compositions was either very low (efficacy values in 5 hives ranging from 19.4% to 44.5%, average: 31.8%) or resulted in the death of the hive.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Insects & Arthropods (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Medicinal Preparation (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Catching Or Destruction (AREA)

Abstract

Bande (10) pour libérer une composition antiparasitaire pour des abeilles pour lutter contre le Varroa dans les ruches. La bande (10) a une surface (A) à imprégner avec une composition antiparasitaire, ayant des ouvertures traversantes (21) pour recevoir la substance, ayant une première partie et une seconde partie (22, 23) pour la substance et une partie intermédiaire (24) entre les première et seconde parties (22, 23) et plus étroite que celles-ci. Une section transversale de l'intérieur des ouvertures traversantes (21) comprend des parois (21a, 21b) inclinées selon un angle (α) l'une par rapport à l'autre. L'invention concerne également un produit prêt à l'emploi comprenant la bande (10) et une composition antiparasitaire vétérinaire pour le traitement ou la prévention d'une infection parasitaire dans une ruche.
PCT/EP2024/061440 2023-04-26 2024-04-25 Bande pour libérer une composition antiparasitaire pour des abeilles pour lutter contre des infections parasitaires dans des ruches Pending WO2024223764A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MX2025012632A MX2025012632A (es) 2023-04-26 2025-10-22 Tira para liberar una composicion antiparasitaria a abejas para controlar infecciones parasitarias en colmenas

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP23382384 2023-04-26
EP23382384.8 2023-04-26

Publications (1)

Publication Number Publication Date
WO2024223764A1 true WO2024223764A1 (fr) 2024-10-31

Family

ID=86272557

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/EP2024/061440 Pending WO2024223764A1 (fr) 2023-04-26 2024-04-25 Bande pour libérer une composition antiparasitaire pour des abeilles pour lutter contre des infections parasitaires dans des ruches
PCT/EP2024/061425 Pending WO2024223754A1 (fr) 2023-04-26 2024-04-25 Composition antiparasitaire pour lutter contre des infections parasitaires dans des ruches

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/EP2024/061425 Pending WO2024223754A1 (fr) 2023-04-26 2024-04-25 Composition antiparasitaire pour lutter contre des infections parasitaires dans des ruches

Country Status (3)

Country Link
AR (2) AR132519A1 (fr)
MX (2) MX2025012632A (fr)
WO (2) WO2024223764A1 (fr)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0441750A1 (fr) * 1990-02-05 1991-08-14 Sandoz Ltd. Amélioration concernant des dispositifs mécaniques
US20070059333A1 (en) * 2005-09-15 2007-03-15 Volby Stuart A Pesticide strips for control of mites in honeybees
WO2015071890A1 (fr) 2013-11-18 2015-05-21 0903608 B.C. Ltd. Compositions, dispositifs et méthodes pour lutter contre des organismes nuisibles à l'aide de vapeurs actives
KR20180001022U (ko) * 2016-10-05 2018-04-13 조상균 꿀벌응애 구제제 스트립용 클립
US20180116198A1 (en) 2013-11-18 2018-05-03 0903608 B.C. Ltd. Compositions, devices and methods for control of pests using vapor activity
WO2020161155A1 (fr) 2019-02-07 2020-08-13 Bayer Animal Health Gmbh Support pour bande de substance active
WO2020172750A1 (fr) * 2019-02-27 2020-09-03 Terramera, Inc. Compositions de lutte contre les organismes nuisibles améliorées exploitant l'activité de la vapeur

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060008492A1 (en) * 2004-07-09 2006-01-12 Pablo Janowicz Composition and method for delivering chemical agent to insects
ES2604527B2 (es) * 2016-11-25 2017-07-07 Agustín ARIAS MARTINEZ Composición y procedimiento multifactorial y ecológico para el tratamiento de la varroosis de las colonias de abejas melíferas, con sistema rápido de aplicación, sin necesidad de abrir la colmena

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0441750A1 (fr) * 1990-02-05 1991-08-14 Sandoz Ltd. Amélioration concernant des dispositifs mécaniques
US20070059333A1 (en) * 2005-09-15 2007-03-15 Volby Stuart A Pesticide strips for control of mites in honeybees
WO2015071890A1 (fr) 2013-11-18 2015-05-21 0903608 B.C. Ltd. Compositions, dispositifs et méthodes pour lutter contre des organismes nuisibles à l'aide de vapeurs actives
US20180116198A1 (en) 2013-11-18 2018-05-03 0903608 B.C. Ltd. Compositions, devices and methods for control of pests using vapor activity
KR20180001022U (ko) * 2016-10-05 2018-04-13 조상균 꿀벌응애 구제제 스트립용 클립
WO2020161155A1 (fr) 2019-02-07 2020-08-13 Bayer Animal Health Gmbh Support pour bande de substance active
WO2020172750A1 (fr) * 2019-02-27 2020-09-03 Terramera, Inc. Compositions de lutte contre les organismes nuisibles améliorées exploitant l'activité de la vapeur

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ANON.: "APITRAZ 500 MG STRIPS FOR BEES - Razimbaud Pharmacy", 11 May 2018 (2018-05-11), pages 1 - 6, XP093102710, Retrieved from the Internet <URL:https://mapharmanaturelle.com/en/online-pharmacy/our-products/veterinary-en/anti-puces-et-anti-tiques-en/apitraz-500-mg-strips-for-bees/> [retrieved on 20231117] *

Also Published As

Publication number Publication date
AR132519A1 (es) 2025-07-02
MX2025012631A (es) 2025-12-01
MX2025012632A (es) 2025-12-01
AR132520A1 (es) 2025-07-02
WO2024223754A1 (fr) 2024-10-31

Similar Documents

Publication Publication Date Title
DE60032021T2 (de) Verabreichung eines arzneistoffs mittels einer sich phasenumwandelnden formulierung
Sajid et al. Efficacy assessment of soft and hard acaricides against Varroa destructor mite infesting honey bee (Apis mellifera) colonies, through sugar roll method
JPH0131481B2 (fr)
JP7293434B2 (ja) スプレー製品
MX2011005790A (es) Control de parasitos.
BRPI0720500A2 (pt) Composições farmacêuticas e métodos para tratar inflamação em gado e outros animais
TW201919588A (zh) 用於治療外寄生物感染之化合物、其組成物和方法
EP2735229A1 (fr) Produit pour le traitement de familles d&#39;abeilles et procédé pour son application
BRPI0715157A2 (pt) mÉtodo de tratamento e composiÇço antiviral tàpica
US20160067288A1 (en) Honey-Based Gel Composition
JPH03169812A (ja) トリアセチンを含む活性持続性注射用処方剤
KR20120122072A (ko) 꿀벌응애 방제용 겔 조성물
KR101796776B1 (ko) 친환경 꿀벌 방제조성물의 제조방법 및 이에 의해 제조되는 친환경 꿀벌 방제조성물
JP6383361B2 (ja) ミツバチヘギイタダニ（Ｖａｒｒｏａｍｉｔｅ）防除用成形品
WO2024223764A1 (fr) Bande pour libérer une composition antiparasitaire pour des abeilles pour lutter contre des infections parasitaires dans des ruches
US20090131539A1 (en) Mixture
JP2002193717A (ja) 抗菌剤
AU2008339883B2 (en) Domestic animal parasite-repellent device
JP2018021004A (ja) ロキソプロフェンを含有してなる医薬製剤
RU2145476C1 (ru) Препарат &#34;бипин-т&#34; для борьбы с варроатозом пчел
KR20190042835A (ko) 쿼세틴을 이용한 꿀벌응애 구제용 제제
EP4413861A1 (fr) Composition pour lutter contre l&#39;infestation par des acariens
CA2939659C (fr) Composition comprenant des produits d&#39;abeille
US12064461B2 (en) Deep penetrating topical cannabinoid compositions and methods for treating musculoskeletal inflammation and pain
ES2879239T3 (es) Agente para el tratamiento de insectos voladores, en particular, abejas melíferas, que están infestados por ácaros

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24722220

Country of ref document: EP

Kind code of ref document: A1

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112025023058

Country of ref document: BR

WWE Wipo information: entry into national phase

Ref document number: 2024722220

Country of ref document: EP