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WO2024217580A1 - Tube de réactif, dispositif de rupture de paroi latérale ultrasonore et procédé de rupture de paroi latérale ultrasonore - Google Patents

Tube de réactif, dispositif de rupture de paroi latérale ultrasonore et procédé de rupture de paroi latérale ultrasonore Download PDF

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Publication number
WO2024217580A1
WO2024217580A1 PCT/CN2024/088990 CN2024088990W WO2024217580A1 WO 2024217580 A1 WO2024217580 A1 WO 2024217580A1 CN 2024088990 W CN2024088990 W CN 2024088990W WO 2024217580 A1 WO2024217580 A1 WO 2024217580A1
Authority
WO
WIPO (PCT)
Prior art keywords
tube
extrusion
reagent tube
assembly
ultrasonic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/CN2024/088990
Other languages
English (en)
Chinese (zh)
Inventor
戴立忠
解亚平
米旭君
郑典苏
朱滔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hunan Biometa Intelligent Manufacturing Technology Co Ltd
Original Assignee
Hunan Biometa Intelligent Manufacturing Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN202310429326.5A external-priority patent/CN118807861A/zh
Priority claimed from CN202320904854.7U external-priority patent/CN220027058U/zh
Priority claimed from CN202310430912.1A external-priority patent/CN118807862A/zh
Priority claimed from CN202320904032.9U external-priority patent/CN220027057U/zh
Priority claimed from CN202320909698.3U external-priority patent/CN220194894U/zh
Priority claimed from CN202320901967.1U external-priority patent/CN219984727U/zh
Priority claimed from CN202321041302.4U external-priority patent/CN220056819U/zh
Priority claimed from CN202321037366.7U external-priority patent/CN220027060U/zh
Priority claimed from CN202321041867.2U external-priority patent/CN220056820U/zh
Priority claimed from CN202321037612.9U external-priority patent/CN220027061U/zh
Priority claimed from CN202311372389.8A external-priority patent/CN118360148A/zh
Priority claimed from CN202323102205.4U external-priority patent/CN221371159U/zh
Priority claimed from CN202323102349.XU external-priority patent/CN221275781U/zh
Priority claimed from CN202323102364.4U external-priority patent/CN221275782U/zh
Priority claimed from CN202323107003.9U external-priority patent/CN221230604U/zh
Application filed by Hunan Biometa Intelligent Manufacturing Technology Co Ltd filed Critical Hunan Biometa Intelligent Manufacturing Technology Co Ltd
Publication of WO2024217580A1 publication Critical patent/WO2024217580A1/fr
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/80—Mixing by means of high-frequency vibrations above one kHz, e.g. ultrasonic vibrations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler

Definitions

  • the present application belongs to the technical field of detection instruments, and in particular, relates to a reagent tube and an ultrasonic side wall breaking device.
  • testers When conducting biological or medical tests, testers usually use reagent tubes to store and transfer reagents or liquid samples, so as to drop the reagents or liquid samples into the interior of the reagents to be tested for detection and analysis.
  • testers When using the reagent tubes in the prior art to transfer reagents or samples, testers mostly need to perform quantitative pipetting by human judgment.
  • the prior art CN207307892U discloses a disposable plastic graduated dropper, which is provided with a liquid capsule; the straw is connected to the upper end of the liquid capsule, and the lower end of the straw is equipped with a second valve; the dripper is integrally installed at the bottom of the liquid capsule, and the lower end of the dripper is equipped with a first valve; the scale line is printed on the outer wall of the liquid capsule.
  • the operator squeezes the airbag and uses the human eye to observe whether the reagent or liquid sample is flush with the scale line on the liquid capsule to achieve quantitative pipetting.
  • the main purpose of the present application is to propose a reagent tube and an ultrasonic side wall breaking device, aiming to solve the technical problem of poor accuracy of quantitative pipetting of reagent tubes in the prior art.
  • the present application provides a reagent tube for use in a detection device, the reagent tube comprising: a tube body assembly, the interior of the tube body assembly is formed with a receiving cavity for receiving liquid; a dripper, detachably arranged on the tube body assembly The first end of the component is formed with a liquid outlet for communicating with the accommodating cavity; the cover body is detachably mounted on the outer peripheral side of the dripper and is used to block the liquid outlet; a quantitative extrusion space is formed between the extrusion component and the tube body component, and the extrusion component is used to squeeze the tube body component so that the liquid in the accommodating cavity flows out quantitatively through the liquid outlet.
  • the dripper and the tube body assembly are detachably connected, the dripper and the tube body assembly can be detached, and after the sample liquid is placed into the receiving chamber through the opening of the tube body assembly, the dripper and the tube body assembly are connected.
  • the cover body and the dripper can be threadedly connected so that the cover body blocks the liquid outlet.
  • the cover body can be removed from the dripper. Since a quantitative extrusion space is formed between the tube body assembly and the extrusion assembly, the extrusion assembly has a quantitative displacement space.
  • the liquid in the receiving chamber can be quantitatively discharged through the liquid outlet, thereby realizing quantitative addition of the reagent tube, avoiding the situation of manual observation to determine whether it is quantitative, and having high quantitative accuracy and convenient operation.
  • FIG1 is a schematic diagram of the structure of a reagent tube according to an embodiment of the present application.
  • FIG2 is a schematic diagram of the cross-sectional structure of a reagent tube according to an embodiment of the present application.
  • FIG3 is a schematic diagram of a cross-sectional structure of a reagent tube according to another embodiment of the present application.
  • FIG4 is a partial structural schematic diagram of FIG3 ;
  • FIG5 is a schematic diagram of a cross-sectional structure of a reagent tube according to an embodiment of the present application.
  • FIG6 is a partial structural schematic diagram of FIG5
  • FIG7 is another partial structural schematic diagram of FIG5;
  • FIG8 is a schematic diagram of a partial structure of a reagent tube according to an embodiment of the present application.
  • FIG9 is a schematic diagram of the structure of an extrusion assembly of a reagent tube according to an embodiment of the present application.
  • FIG10 is a schematic diagram of the structure of an annular pressing member of a reagent tube according to an embodiment of the present application.
  • FIG. 11 is a schematic diagram of the structure of a stopper for a reagent tube according to an embodiment of the present application.
  • FIG12 is a schematic diagram of the structure of a reagent tube according to an embodiment of the present application.
  • FIG13 is a schematic diagram of the cross-sectional structure of FIG12;
  • FIG14 is a schematic diagram of the structure of a reagent tube according to another embodiment of the present application.
  • FIG15 is a schematic cross-sectional view of the structure of FIG14;
  • FIG16 is a schematic diagram of the structure of a reagent tube according to another embodiment of the present application.
  • FIG17 is a schematic diagram of the cross-sectional structure of FIG16.
  • FIG18 is a schematic diagram of the structure of a reagent tube according to yet another embodiment of the present application.
  • FIG19 is a schematic diagram of a cross-sectional structure of a reagent tube according to an embodiment of the present application.
  • FIG20 is a schematic diagram of a cross-sectional structure of a reagent tube according to another embodiment of the present application.
  • FIG21 is a schematic diagram of the structure of a reagent tube according to an embodiment of the present application.
  • FIG22 is a schematic diagram of the cross-sectional structure of FIG21;
  • FIG23 is a schematic diagram of a dripper structure of a reagent tube according to an embodiment of the present application.
  • FIG24 is a schematic diagram of the structure of an extrusion assembly of a reagent tube according to an embodiment of the present application.
  • FIG25 is a schematic diagram of the structure of an extrusion assembly of a reagent tube according to another embodiment of the present application.
  • FIG26 is a schematic diagram of the structure of an ultrasonic side wall breaking device according to an embodiment of the present application.
  • FIG. 27 is a schematic structural diagram of an ultrasonic generating mechanism in an ultrasonic side wall breaking device according to an embodiment of the present application.
  • FIG28 is a schematic diagram of the structure of a mounting block in a clamping assembly according to an embodiment of the present application.
  • FIG29 is a schematic diagram of the structure of a reagent tube according to an embodiment of the present application.
  • FIG30 is a schematic diagram of a cross-sectional structure of a dripper according to an embodiment of the present application.
  • FIG31 is a schematic diagram of the structure of a reagent tube according to another embodiment of the present application.
  • FIG32 is a schematic diagram of the exploded structure of a reagent tube according to another embodiment of the present application.
  • FIG33 is a schematic diagram of a partial cross-sectional structure of a reagent tube according to another embodiment of the present application.
  • FIG34 is a schematic diagram of a partial structure of a reagent tube according to another embodiment of the present application.
  • FIG35 is a schematic diagram of a cross-sectional structure of a reagent tube according to an embodiment of the present application.
  • Figure 36 is a schematic diagram of the tube body assembly structure of a reagent tube according to one embodiment of the present application.
  • the reagent tube 100 includes a tube body assembly 1, a dripper 2, a cover body 3 and an extrusion assembly 4, wherein a accommodating chamber 11 for accommodating liquid is formed inside the tube body assembly 1; the dripper 2 is detachably arranged at the first end of the tube body assembly 1 and is formed with a liquid outlet 21 for communicating with the accommodating chamber 11; the cover body 3 is detachably mounted on the outer peripheral side of the dripper 2 and is used to block the liquid outlet 21; a quantitative extrusion space 5 is formed between the extrusion assembly 4 and the tube body assembly 1, and the extrusion assembly 4 is used to extrude the tube body assembly 1 so that the liquid in the accommodating chamber 11 flows out quantitatively through the liquid outlet 21.
  • the reagent tube 100 in this embodiment is mainly used for nucleic acid detection in detection equipment. After the sample is sampled by the sampling swab 300, it can be placed in the containing cavity 11 of the tube body component 1 to form a sample solution.
  • the dripper 2 and the tube body assembly 1 are detachably connected, and the dripper 2 and the tube body assembly 1 can be separated.
  • the sample liquid is placed into the containing chamber 11 through the opening of the tube body assembly 1, the dripper 2 and the tube body assembly 1 are connected.
  • the cover body 3 and the dripper 2 can be threadedly connected so that the cover body 3 blocks the liquid outlet 21.
  • the cover body 3 can be removed from the dripper 2. Since a quantitative squeezing space 5 is formed between the tube body assembly 1 and the squeezing assembly 4, the squeezing assembly 4 has a quantitative displacement space.
  • the liquid in the containing chamber 11 can be quantitatively discharged through the liquid outlet 21, thereby realizing quantitative sampling of the reagent tube 100, avoiding the situation of manual observation to determine whether it is quantitative, and having high quantitative accuracy and convenient operation.
  • the extrusion component 4 is a flexible vesicle portion disposed at the second end of the tube body component 1 , and the quantitative extrusion space 5 is an extrusion cavity formed inside the flexible vesicle portion, and the extrusion cavity is connected to the accommodating cavity 11 .
  • the inspector inverts the reagent tube 100 as a whole and presses the flexible vesicle part (in this embodiment, an electric push rod or a hydraulic push rod or other equipment can also be used to press the flexible vesicle part) to deform the flexible vesicle part and move it toward the direction of the accommodating cavity 11.
  • the flexible vesicle part in this embodiment, an electric push rod or a hydraulic push rod or other equipment can also be used to press the flexible vesicle part
  • the extrusion cavity becomes smaller, that is, the space inside the reagent tube 100 (the interior of the reagent tube 100 includes the accommodating cavity 11, the extrusion cavity and the pipetting channel) becomes smaller, so that the air pressure inside the reagent tube 100 increases, and the liquid inside the reagent tube 100 can be discharged outward through the liquid outlet 21.
  • a circular connecting portion 101 for connecting to the tube body assembly 1 is provided on the outer peripheral side of the flexible vesicle portion.
  • the volume of the extrusion cavity is 0 at this time, so that the change amount of the extrusion cavity is a fixed value (the fixed value is the volume value of the extrusion cavity before the flexible vesicle portion is pressed). Therefore, after the flexible vesicle portion is pressed to deform it and place it in the same plane with the circular connecting portion 101, the space inside the reagent tube 100 can only be reduced by a preset size.
  • the liquid inside the reagent tube 100 (the liquid here refers to the mixture of the sample and the reagent) can only be squeezed outward by a preset volume, thereby realizing the quantitative pipetting function of the reagent tube 100.
  • the reagent tube 100 has a simple structure. When the inspector uses it for pipetting, the quantitative pipetting can be realized by pressing the flexible vesicle portion into a plane. It is not easy to produce pipetting deviation due to fatigue operation, which can better ensure the accuracy of quantitative pipetting and improve the accuracy of pipetting.
  • the reagent tube 100 further includes a stopper 12 disposed in the accommodating cavity 11 and used to limit the extrusion depth of the flexible vesicle portion.
  • a quantitative extrusion space 5 is formed between the flexible vesicle portion and the stopper 12.
  • the stopper 12 is further formed with a plurality of through holes 121 that connect the extrusion cavity and the accommodating cavity 11.
  • the liquid inside the reagent tube 100 (the liquid here refers to the mixture of the sample and the reagent) can only be squeezed out of a preset volume.
  • the reagent tube 100 has a simple structure and can detect When pipetting, personnel can achieve quantitative pipetting without controlling the squeezing force of the flexible vesicle part. No human judgment is required, and pipetting deviation will not occur due to fatigue operation. The accuracy of quantitative pipetting can be better guaranteed, and the precision of pipetting is improved.
  • a mounting groove 122 is formed on the side wall of the stop member 12 facing away from the dripper 2, an annular mounting portion 41 is arranged on the periphery of the flexible vesicle portion, and the annular mounting portion 41 is arranged in the mounting groove 122, and the extrusion assembly 4 also includes an annular clamping member 42 connected to the tube body assembly 1, and the annular clamping member 42 is used to apply a clamping force to the annular mounting portion 41.
  • multiple through holes 121 are all within the coverage range of the flexible vesicle portion, and the annular pressing member 42 applies a pressing force to the annular mounting portion 41 while being connected to the second end of the tube body assembly 1.
  • the annular mounting portion 41 is tightly pressed in the mounting groove 122 under the action of the pressing force, that is, the annular pressing member 42 realizes the installation connection between the extrusion assembly 4 and the tube body assembly 1; in addition, the use of this structural form can reduce the restrictions on the manufacturing materials of the extrusion assembly 4 and the tube body assembly 1, and expand the selection range of the manufacturing materials of the extrusion assembly 4 and the tube body assembly 1.
  • the flexible vesicle portion is made of silicone material and the tube body assembly 1 is made of polypropylene material (i.e. PP material) or polyethylene material (i.e. PE material), the above-mentioned structural form can still achieve a stable connection between the two.
  • polypropylene material i.e. PP material
  • PE material polyethylene material
  • the annular clamping member 42 includes a welding portion 421 and a clamping portion 422 for clamping the annular mounting portion 41 in the mounting groove 122 .
  • the welding portion 421 is arranged on the lower end surface of the clamping portion 422 and is used to be welded to the pipe body assembly 1 .
  • the annular clamping member 42 applies a clamping force to the annular mounting portion 41 through the clamping portion 422; the welding portion 421 is arranged on the lower end surface of the clamping portion 422 and is used to be welded together with the tube body assembly 1.
  • the tube body assembly 1 also includes a cylindrical portion detachably connected to the first end and the dripper 2, and the stop member 12 is arranged inside the second end of the cylindrical portion.
  • the welding portion 421 is an annular welding line arranged on the bottom wall of the clamping portion 422 facing the tube body assembly 1. The welding between the clamping portion 422 and the end surface of the second end of the cylindrical portion can be achieved by heating the annular welding line.
  • the tube body assembly 1 includes a main body 13 and an extrusion body 14, the first end of the main body 13 is detachably connected to the dripper 2, the flexible vesicle portion is formed on the extrusion body 14, the extrusion body 14 also includes a mounting portion 141 arranged on the periphery of the vesicle portion, an annular mounting cavity 1411 is formed on the mounting portion 141, and the second end of the main body 13 is provided with an annular insertion portion 131 for matching with the annular mounting cavity 1411.
  • the annular insertion portion 131 is placed in the annular mounting cavity 1411 to achieve a quick connection between the extrusion body 14 and the main body 13.
  • the main body 13 and the extrusion body 14 are integrally injection molded.
  • the integral injection molding process in this embodiment is the same as the integral injection molding process in the prior art.
  • the manufacturing material for the extrusion body 14 is first injected into the mold of the extrusion body 14 to complete the production of the extrusion body 14;
  • the mold of the main body 13 includes two parts, one of which is the extrusion body 14 (the annular mounting cavity 1411 on the extrusion body 14 is used as the mold of the annular insertion portion 131 of the main body 13),
  • the other part is a cylindrical mold.
  • the two parts of the mold are assembled together, and then the manufacturing material of the main body 13 is injected into it to complete the production of the main body 13.
  • the tube body assembly 1 made in this way has good sealing performance, and there is no gap between the main body 13 and the extrusion body 14.
  • the liquid will not leak from between the main body 13 and the extrusion body 14, which improves the reliability of the use of the reagent tube 100 and is also conducive to further improving the accuracy of quantitative pipetting.
  • an inverted truncated cone-shaped liquid outlet 22 is formed on the dripper 2, and the cover body 3 includes a protective portion 31 for protecting the liquid outlet 22, and an inclined support wall 311 for supporting the peripheral wall of the liquid outlet 22 is formed on the inner side of the protective portion 31.
  • the cover body 3 When the reagent tube 100 is not performing a pipetting operation, the cover body 3 is sleeved on the dripper 2 to prevent the dripper 2 from being accidentally damaged due to external reasons (such as falling on the ground and being hit); when the reagent tube 100 is performing a pipetting operation, the cover body 3 and the dripper 2 are disassembled to expose the liquid outlet 21 so that the liquid to be transferred and/or detected can flow out. Further, the liquid outlet 22 is set to an inverted truncated cone shape (this shape is the shape of the liquid outlet 22 when the reagent tube 100 is in a working state), and when the cover body 3 is tightened on the dripper 2, the liquid outlet 22 enters the protective part 31 of the cover body 3.
  • the bottom wall of the protective part 31 contacts the bottom wall of the liquid outlet 22, and the protective part 31 blocks the liquid outlet 21.
  • the inclined support wall 311 on the protective part 31 will support the surrounding wall of the liquid outlet part 22.
  • the extrusion force between the bottom wall of the protective part 31 and the bottom wall of the liquid outlet part 22 is reduced, so that the liquid outlet end of the liquid outlet part 22 (that is, the bottom end face where the liquid outlet 21 is located) is subjected to less force, thereby reducing the risk of the dripper 2 being damaged due to the extrusion force, that is, the cover body 3 further enhances the protection effect of the dripper 2.
  • the inclined support wall 311 is in the shape of an inverted truncated cone and can fit with the outer peripheral wall of the liquid outlet 22.
  • This structural design can increase the contact area between the inclined support wall 311 and the outer peripheral wall of the liquid outlet 22 as much as possible when they are in contact, which can avoid the situation where the liquid outlet 22 is subjected to concentrated force, and can also make the force of the liquid outlet 22 more dispersed and uniform, which is conducive to further improving the protective effect of the cover body 3 on the liquid outlet 22.
  • the tube body assembly 1 includes an inner hose 15 and an outer support tube 16, the accommodating chamber 11 is arranged in the inner hose 15, the outer support tube 16 is sleeved outside the inner hose 15 and is used to protect the inner hose 15, the extrusion assembly 4 is used to extend into the outer support tube 16 and extrude the inner hose 15, and a quantitative extrusion space 5 is formed between the extrusion assembly 4 and the outer support tube 16.
  • the hardness of the outer support tube 16 is greater than that of the inner hose 15, and the outer support tube 16 can protect the inner hose 15 to prevent the inner hose 15 from leaking liquid or transferring excessive liquid due to accidental touch.
  • the sampling swab 300 is placed in the accommodating cavity 11 of the inner hose 15, and the entire reagent tube 100 is shaken to fully mix the sampling swab 300 with the liquid inside the accommodating cavity 11.
  • External force can be applied to the pressing component by manual drive, hydraulic drive or mechanical drive, so that the liquid in the inner hose 151 flows out from the liquid outlet 21.
  • the pressing component can be arranged on the side or end of the inner hose 15.
  • a support gap 17 is formed between the outer support tube 16 and the inner hose 15, and the outer support tube
  • An anti-slip structure 18 is provided between the inner hose 16 and the inner hose 15 , and the anti-slip structure 18 is used to resist the side tube wall of the inner hose 15 along the extension direction of the inner hose 15 .
  • the outer support tube 16 in this embodiment can be first sleeved on the outside of the inner hose 15 to form a support gap 17 between the inner hose 15 and the outer support tube 16, which can avoid the outer support tube 16 contacting the inner hose 15 with a large area and causing inaccurate pipetting control, and an anti-detachment structure 18 is provided between the inner hose 15 and the outer support tube 16 to avoid the double-layer sleeve from detaching, thereby ensuring the stability of the reagent tube 100.
  • the pressing component is passed through the outer support tube 16. After the liquid preparation is completed, an external force can be applied to the pressing component to squeeze the inner hose 15.
  • the inner hose 15 Since the contact area between the pressing component and the inner hose 15 is constant, the inner hose 15 is squeezed by a fixed volume, so that the volume of the droplets squeezed out each time reaches the quantitative requirement, which meets the quantitative requirement and is more conducive to accurate detection.
  • the outer support tube 16 and the inner soft tube cooperate with each other to form a double-layer sleeve, and an anti-slip structure 18 is set between the outer support tube 16 and the inner soft tube 15 to form a support gap 17.
  • an anti-slip structure 18 is set between the outer support tube 16 and the inner soft tube 15 to form a support gap 17.
  • the anti-slip structure 18 includes a hose convex strip and a support convex strip, wherein the hose convex strip is arranged on the outer tube wall of the inner hose 15, and the support convex strip is arranged on the inner wall of the outer support tube 16.
  • the contact area between the hose convex strip and the support convex strip is relatively small, and the outer surfaces of the hose convex strip and the support convex strip are both arc-shaped surfaces.
  • the support convex strip is located between the hose convex strip and the liquid outlet 21, and can resist the hose convex strip in the direction close to the liquid outlet 21, and resist the side tube wall of the inner hose 15 in the up and down directions, so as to prevent the outer support tube 16 from excessively squeezing the inner hose 15, resulting in a large amount of liquid being discharged from the inner hose 15 in a short time.
  • the inner hose 15 includes a tube body portion 151 and a pressing portion 152 connected in sequence
  • the accommodating chamber 11 is arranged in the tube body portion 151
  • the pressing portion 152 is sealed at one end of the accommodating chamber 11
  • the extrusion assembly 4 includes a pressing block 43 and a pressing column 44 connected to the pressing block 43
  • the pressing block 43 is located outside the outer support tube 16 and is used to reciprocate between an initial position and a quantitative position
  • an axial quantitative extrusion space 5 is formed between the pressing block 43 located at the initial position and the outer support tube 16, and the pressing block 43 located at the quantitative position abuts against the end of the outer support tube 16; when moving from the initial position to the quantitative position, the pressing block 43 axially squeezes the pressing portion 152 to quantitatively squeeze out the liquid in the accommodating chamber 11.
  • the outer support tube 16 in this embodiment can be first sleeved outside the inner hose 15, so that the tube side wall of the inner hose 15 is supported on the tube side wall of the outer support tube 16, and the inner hose 15 can be protected by the outer support tube 16.
  • the outer support tube 16 and the inner hose 15 cooperate with each other to form a double-layer sleeve, which can avoid the situation where the inner hose 15 is accidentally touched and the liquid is squeezed out.
  • the pressing part 152 seals the other end of the accommodating chamber 11 to avoid liquid leakage.
  • the pressing column 44 can be extended into the outer support tube 16 to keep the pressing block 43 in the initial position outside the pressing frame.
  • An external force can be applied to the pressing block 43 until the pressing block 43 moves to a quantitative position, and the pressing block 43 abuts against the outer support tube 16.
  • the pressing column 44 squeezes the pressing part 152 to deform the pressing part 152. Since the length of the quantitative extrusion space 5 is constant, the fixed pressing stroke of the fixed pressing block 43 is fixed to make each The volume of the liquid droplets squeezed out for the first time reaches the quantitative requirement, which meets the requirement of liquid quantification and is more conducive to accurate detection.
  • the outer support tube 16 protects the inner hose 15 and limits the pressing block 43, which can avoid leakage caused by accidentally touching the tube body 151.
  • the pressing block 43 By pressing the pressing block 43 to the quantitative position at one time, accurate quantitative pipetting can be performed quickly without human observation, thereby improving the efficiency and accuracy of quantitative pipetting.
  • the inner hose 15 also includes a connecting frame 153, the pressing portion 152 is connected between the connecting frame 153 and the tube body 151, the connecting frame 153 is connected to the pressing column 44 through a limiting structure 19, and the limiting structure 19 is used to limit the pressing column 44 and the connecting frame 153 along the axial direction of the tube body 151.
  • the connecting frame 153 and the pressing column 44 are connected by a limiting structure 19.
  • the limiting structure 19 limits the connecting frame 153 and the pressing column 44, which can prevent the pressing column 44 from being offset relative to the connecting frame 153 and ensure the quantitative stroke of the pressing block 43 in the vertical direction.
  • the pressing column 44 can rotate in the horizontal direction relative to the connecting frame 153.
  • the tube body assembly 1 includes a accommodating portion 10 and a connecting portion 101 arranged at one end of the accommodating portion 10, the accommodating portion 10 is internally formed with an accommodating cavity 11, the connecting portion 101 is internally formed with a piston cavity 1011 connected to the accommodating cavity 11, the extrusion assembly 4 includes a piston rod 45 and a limiting cylinder 46 connected to the piston rod 45, part of the piston rod 45 is movably arranged in the piston cavity 1011, a quantitative extrusion space 5 is formed between the limiting cylinder 46 and the accommodating portion 10, and a limiting end surface 461 is formed on the limiting cylinder 46 for resisting on the side end surface of the accommodating portion 10 facing the limiting cylinder 46.
  • the inspector presses the piston rod 45 downward (in this embodiment, an electric push rod or a hydraulic push rod or other equipment can also be used to press the piston rod 45), and the piston rod 45 moves toward the direction of the accommodating chamber 11, the volume of the columnar cavity becomes smaller, and the space inside the tube body becomes smaller, so that the internal air pressure of the reagent tube 100 increases, and the liquid inside the reagent tube 100 can be discharged outward through the liquid outlet 21.
  • the piston rod 45 when the piston rod 45 moves, it drives the limiting cylinder 46 to move together, and the moving strokes of the two are consistent.
  • the piston rod 45 can only move a preset distance, that is, the space inside the reagent tube 100 can only be reduced by a preset size.
  • the liquid inside the reagent tube 100 can only squeeze out a preset volume, thereby realizing the quantitative liquid transfer function of the reagent tube 100.
  • the reagent tube 100 has a simple structure. When using it for pipetting, the tester can achieve quantitative pipetting without controlling the squeezing force of the piston rod 45. No human judgment is required, and there will be no pipetting deviation due to fatigue operation. It can better ensure the accuracy of quantitative pipetting and improve the precision of pipetting.
  • the extrusion assembly 4 further includes a seal 47 sleeved on the piston rod 45 and located in the piston cavity 1011.
  • the seal 47 in this embodiment is a sealing ring, which is used to enhance the sealing performance inside the reagent tube 100 to avoid leakage or air leakage, which is conducive to further improving the accuracy of pipetting.
  • a limiting step 1012 is formed on the outer peripheral wall of the connecting portion 101 , the limiting cylinder 46 is arranged on the outer peripheral side of the piston rod 45 and the connecting portion 101 , and a limiting block portion for resisting against the limiting step 1012 is formed on the inner peripheral wall of the limiting cylinder 46 .
  • the limit step 1012 is in the shape of a circular ring.
  • the limit block part stops moving under the blocking action of the limit step 1012, and accordingly, the limit cylinder 46 and the piston rod 45 also stop moving, thereby limiting the moving distance of the limit cylinder 46 and the piston rod 45 when they are reset, avoiding the piston rod 45 from moving too long when it is reset and escaping from the piston cavity 1011, thereby improving the structural stability and reliability of the extrusion assembly 4.
  • a limiting boss 102 is formed on the outer circumferential wall of the tube body assembly 1
  • the extrusion assembly 4 is sleeved on the second end of the tube body assembly 1 and can move axially toward the limiting boss 102
  • a quantitative extrusion space 5 is formed between the extrusion assembly 4 and the limiting boss 102
  • extrusion ribs 48 for applying lateral extrusion force to the tube body assembly 1 are formed on the inner circumferential wall of the extrusion assembly 4.
  • the inspector pushes the extrusion assembly 4 (in this embodiment, an electric push rod or a hydraulic push rod or other equipment can also be used to push the extrusion assembly 4), and the extrusion assembly 4 moves in the direction of the limiting boss 102.
  • the extrusion rib 48 applies a lateral extrusion force to the tube body assembly 1 to deform the tube body assembly 1.
  • the volume of the accommodating cavity 11 becomes smaller, and the space inside the reagent tube 100 becomes smaller, so that the internal air pressure of the reagent tube 100 increases, and the liquid inside the reagent tube 100 can be discharged outward through the liquid outlet 21.
  • the extrusion assembly 4 moves a preset distance relative to the tube body assembly 1, it will be blocked by the limiting boss 102. At this time, no matter how much force the inspector applies to the tube body assembly 1, the tube body assembly 1 cannot continue to move. Therefore, the tube body assembly 1 can only move a preset distance relative to the tube body assembly 1, that is, the space inside the reagent tube 100 can only be reduced by a preset size. Accordingly, the liquid inside the reagent tube 100 can only be squeezed out of the quantitative extrusion space 5, thereby realizing the quantitative liquid transfer function of the reagent tube 100.
  • the reagent tube 100 has a simple structure. When using it for pipetting, the tester can achieve quantitative pipetting without controlling the force applied to the extrusion component 4. No human judgment is required, and no pipetting deviation will occur due to fatigue operation. It can better ensure the accuracy of quantitative pipetting and improve the precision of pipetting.
  • the tube body assembly 1 includes a pressure-bearing portion 103 for bearing lateral extrusion force and a first limiting portion 104 located on the side of the pressure-bearing portion 103 close to the dripper 2.
  • the extrusion rib 48 includes an extrusion portion 481 and a second limiting portion 482. The extrusion portion 481 is used to cooperate with the pressure-bearing portion 103, and the second limiting portion 482 is used to cooperate with the first limiting portion 104.
  • the extrusion portion 481 of the extrusion rib 48 applies lateral extrusion force to the pressure-bearing portion 103, and the extrusion portion 481 is deformed, thereby causing the volume of the accommodating chamber 11 to change; the second limiting portion 482 cooperates with the first limiting portion 104, an inclined first limiting surface is formed on the first limiting portion 104, and an inclined second limiting surface is formed on the second limiting portion 482.
  • the second limiting portion 482 is in a state of being ...
  • a limiting surface and a second limiting surface are in contact and extrusion occurs, that is, the first limiting portion 104 can also realize the movement of the extrusion assembly 4 by blocking the movement of the second limiting portion 482, further ensuring the accuracy of the moving distance of the extrusion assembly 4.
  • a limiting groove 105 is formed on the outer peripheral wall of the tube body assembly 1, and the limiting groove 105 is arranged along the axial direction of the tube body assembly 1.
  • the extrusion assembly 4 is sleeved on the second end of the tube body assembly 1, and an extrusion strip 49 and a limiting protrusion 40 are formed on the inner peripheral wall of the extrusion assembly 4.
  • the extrusion strip 49 is used to apply lateral extrusion force to the tube body assembly 1, and the limiting protrusion 40 is movably arranged in the limiting groove 105.
  • the tester first pulls the extrusion assembly 4 to move away from the dripper 2 so that the limiting clamp 40 abuts against the side wall of the limiting groove 105 away from the end of the dripper 2, and then uses the sampling swab 300 to complete the nucleic acid sampling.
  • the dripper 2 is unscrewed to place the sampling swab 300 in the receiving chamber 11, and then the dripper 2 is tightened and the reagent tube 100 is shaken as a whole to fully mix the nucleic acid in the sampling swab 300 and the liquid in the receiving chamber 11. After the nucleic acid and the liquid in the receiving chamber 11 are fully mixed, the reagent tube 100 is inverted as a whole.
  • the air pressure outside the reagent tube 100 and the pressure inside the reagent tube 100 reach a balance, and the inside of the reagent tube 100 forms a relatively sealed state. Therefore, even if the reagent tube 100 is inverted as a whole, the liquid inside the reagent tube 100 will not leak out through the liquid outlet 21.
  • the inspector presses the extrusion assembly 4 (in this embodiment, an electric push rod or a hydraulic push rod or other equipment can also be used to press the extrusion assembly 4) to move the limiting protrusion 40 in the limiting groove 105 toward the direction close to the dripper 2.
  • the extrusion bar 49 applies lateral extrusion force to the tube body assembly 1 to deform the tube body assembly 1.
  • the volume of the accommodating cavity 11 becomes smaller, and the space inside the reagent tube 100 becomes smaller, so that the air pressure inside the reagent tube 100 increases, and the liquid inside the reagent tube 100 can be discharged outward through the liquid outlet 21101.
  • the length of the limiting groove 105 is a fixed value, when the extrusion component 4 moves a preset distance relative to the tube body component 1 (the preset distance is consistent with the length of the limiting groove 105), the side wall of the limiting groove 105 close to the end of the dripper 2 will block the limiting convex 40. At this time, no matter how much force the inspector applies to the extrusion component 4, the extrusion component 4 cannot continue to move. Therefore, the extrusion component 4 can only move a preset distance relative to the tube body component 1, that is, the space inside the reagent tube 100 can only be reduced by a preset size.
  • the liquid inside the reagent tube 100 can only be squeezed out of a preset volume, thereby realizing the quantitative pipetting function of the reagent tube 100.
  • the reagent tube 100 has a simple structure. When the inspector uses it for pipetting, there is no need to control the force applied to the extrusion component 4 to achieve quantitative pipetting. There is no need for human judgment, and there will be no pipetting deviation due to fatigue operation. It can better ensure the accuracy of quantitative pipetting and improve the accuracy of pipetting.
  • the reagent tube 100 further includes an elastic reset member 6 for restoring the extrusion assembly 4 to its original position, thereby facilitating the next pipetting operation by the testing personnel and improving the ease of use of the reagent tube 100 .
  • the outer wall of the tube body assembly 1 is also formed with a stop step 106, and the elastic reset member 6 is sleeved on the outer peripheral side of the tube body assembly 1 and is located between the stop step and the extrusion strip 49.
  • Step 106 the elastic reset member 6 is sleeved on the outer peripheral side of the tube body assembly 1 and is located between the resisting step 106 and the extrusion strip 49.
  • the elastic reset member 6 in this embodiment can be selected as a spring, and the resisting step 106 is on the side of the limiting groove 105 away from the dripper 2. One end of the spring abuts against the resisting step 106, and the other end of the spring abuts against the extrusion strip 49.
  • the spring Before the inspector presses the extrusion assembly 4, the spring is in an extended state, and the spring applies a thrust to the extrusion strip 49 of the extrusion assembly 4. Under the action of the above thrust, the extrusion assembly 4 is in the first position (the first position is the original position of the extrusion assembly 4). At this time, the limiting protrusion 40 abuts against the limiting protrusion 49.
  • the positioning groove 105 is on the side wall away from one end of the dripper 2 to prevent the volume of the accommodating chamber 11 from changing before the pipetting operation; after the inspector presses the extrusion assembly 4, the pressing force is greater than the elastic force of the spring, the spring is compressed, and the limiting protrusion 40 moves in the limiting groove 105 toward the dripper 2 until the limiting protrusion 40 abuts against the side wall of the limiting groove 105 close to the end of the dripper 2. When the inspector releases the extrusion assembly 4, the spring changes from a compressed state to an extended state, and the extrusion assembly 4 also returns to its original position under the action of the spring extension force.
  • the dripper 2 is provided with a delivery channel 23, a transition channel 24 and a quantitative pipetting channel 25 with an opening which are connected in sequence.
  • the inner diameter of the transition channel 24 is smaller than the inner diameter of the delivery channel 23 and larger than the inner diameter of the transition channel 24.
  • the delivery channel 23 is connected to the accommodating chamber 11.
  • the tube body assembly 1, the dripper 2 and the cover body 3 are sealed and connected in sequence, and the cover body 3 blocks the liquid outlet 21 of the dripper 2, which can avoid liquid leakage and can seal and preserve the liquid.
  • the cover body 3 can be removed relative to the dripper 2, the liquid outlet 21 is opened, and an external force is applied to the extrusion assembly 4, so that the liquid in the accommodating chamber 11 passes through the delivery channel 23, the transition channel 24 and the quantitative pipetting flow 25 in sequence, and finally flows out through the opening of the quantitative pipetting flow 25.
  • the inner diameters of the delivery channel 23, the transition channel 24 and the quantitative pipetting flow 25 are reduced in sequence, among which the delivery channel 23 has the largest inner diameter, which can facilitate the liquid in the accommodating chamber 11 to quickly and smoothly enter the delivery channel 23.
  • the inner diameter of the transition channel 24 is between the inner diameter of the delivery channel 23 and the inner diameter of the quantitative pipetting flow 25, which can buffer the liquid and avoid the situation where the inner diameter suddenly changes and causes the flow to be intercepted.
  • the quantitative pipetting flow 25 with the smallest inner diameter can accurately control the amount of each drop passing through the opening.
  • the reagent tube 100 also includes a filter assembly 5, which is arranged in the dripper 2 and includes a primary filter element 51 and a secondary filter element 52.
  • the primary filter element 51 is used to divide the inside of the dripper 2 into a plurality of diversion channels 53
  • the secondary filter element 52 is a microporous structure and is used to block the outlet of the diversion channel 53.
  • the primary filter 51 is used to divide the flow channel in the dripper 2 into a plurality of mutually independent flow channels 53.
  • the secondary filter 52 is a hydrophobic microporous structure and is arranged at the outlet of the flow channel 53.
  • the secondary filter 52 can intercept impurities and allow the liquid to pass smoothly.
  • the liquid in the accommodating chamber 11 is mixed with large particles or floccules, the liquid will be discharged as the liquid passes through the secondary filter 52.
  • the liquid is discharged from the liquid outlet 21 of the dripper 2, and the large particles or flocs will flow to the primary filter element 51 and randomly enter a diversion channel 53.
  • the secondary filter element 52 will intercept the large particles or flocs at the outlet of the diversion channel 53 to stop the flow of the large particles or flocs, thereby preventing the large particles or flocs from flowing to the liquid outlet 21 of the dripper 2 and causing the liquid outlet 21 to be blocked. Since there are multiple diversion channels 53, when one of the diversion channels 53 is blocked by large particles or flocs, the liquid can be discharged from other diversion channels 53, so that the dropper can discharge the liquid smoothly, ensuring the accuracy of the reagent tube 100.
  • the delivery channel 23 includes a first liquid outlet cavity section and a second liquid outlet cavity section, a primary filter 51 is arranged in the first liquid outlet cavity section, one end of the secondary filter 52 is installed in the second liquid outlet cavity section and is in close contact with the inner wall of the second liquid outlet cavity section, and the other end of the secondary filter 52 is abutted against the primary filter 51 to block the outlet of the diverter channel 53.
  • the secondary filter 52 When the secondary filter 52 is installed, it is necessary to be in close contact with the outlet surface of the diverter channel 53 to achieve the purpose of blocking the outlet of the diverter channel 53, and the secondary filter 52 is preferably interference fit with the second liquid outlet cavity section, and the interference fit can avoid the formation of a gap between the secondary filter 52 and the inner wall of the delivery channel, prevent large particles or flocs from slipping out of the gap, and ensure the reliability of interception by the secondary filter 52.
  • the primary filter element 51 includes a central portion 511 and a plurality of partition portions 512 spaced apart in the circumferential direction of the central portion 511, wherein the partition portion 512 contacts the cavity wall of the first liquid outlet cavity section, wherein the diverter channel 53 is formed by two adjacent partition portions 512 and the cavity wall of the first liquid outlet cavity section.
  • the primary filter element 51 is also preferably interference fit with the first liquid outlet cavity section to ensure the firmness of the installation of the filter assembly 5 and avoid the filter assembly 5 from loosening and slipping after installation, and there are many ways to form the diverter channel 53, such as by the above-mentioned partition enclosure, or by opening a through hole in the primary filter element 51.
  • a vent hole 107 communicating with the accommodating cavity 11 is formed on the side wall of the tube body assembly 1, and the flexible vesicle portion covers the vent hole 107;
  • the reagent tube 100 also includes an ultrasonic conduction head 108 connected to the ultrasonic end of the tube body assembly 1, and the cross-section of the ultrasonic conduction head 108 gradually increases from the ultrasonic end toward the first end of the tube body assembly 1, and a divergent ultrasonic conduction slope 109 is formed.
  • the dripper 2 When the reagent tube 100 is used for sample processing, the dripper 2 can be removed from the tube body component 1, the accommodating chamber 11 penetrates the tube body component 1, and the end away from the ultrasonic conduction head 108 is open, and the solution can be injected into the accommodating chamber 11. After the solution is injected, the dripper 2 and the tube body component 1 can be connected. When ultrasonic treatment is required, the ultrasonic conduction head 108 can be brought into contact with the ultrasonic head.
  • the divergent ultrasonic conduction inclined surface 109 on the ultrasonic conduction head 108 can imitate the ultrasonic divergence to achieve rapid conduction of ultrasonic waves, which can greatly improve the ultrasonic propagation efficiency and quickly shake the solution in the reagent tube 100.
  • the flexible vesicle part can be pressed to deform the flexible vesicle part, and the air in the flexible vesicle part enters the accommodating chamber 11, thereby quantitatively squeezing out the sample through the change in air pressure.
  • the solution in the containing chamber 11 is compressed to make the solution flow out of the reagent tube 100 through the liquid outlet 21 of the dripper 2.
  • the flexible vesicle part of the reagent tube 100 is placed on its side and matched with the ultrasonic conduction head 108 at the ultrasonic end, which can realize quantitative liquid transfer, improve the ultrasonic processing efficiency and shorten the ultrasonic time.
  • the reagent tube 100 also includes a dosage feedback component 6, which includes a baffle 61 and an elastic quantitative frame 62, which are respectively cantilevered to the inner tube wall of the tube body component 1.
  • the cantilever extension end of the elastic quantitative frame 62 is arranged adjacent to the flexible vesicle portion.
  • the pressed flexible vesicle portion can push the cantilever extension end of the elastic quantitative frame 62 to contact and collide with the baffle 61 to form feedback for prompting that the pressure is in place.
  • the air in the flexible vesicle portion can be pressed to allow the air in the flexible vesicle portion to enter the accommodating chamber 11, and the air pressure in the accommodating chamber 11 changes.
  • the solution can flow out of the reagent tube 100 through the liquid outlet 21 under the action of the air pressure.
  • the dosage feedback component 6 close to the flexible vesicle portion can be pressed at the same time, so that the elastic quantitative frame 62 rotates from the initial position relative to the tube body component 1 to the quantitative position, and in the process of rotation, the cantilever extension end of the elastic quantitative frame 62 collides with the stopper 61 to produce a "click" feedback collision sound, which provides tactile feedback to the human hand and acoustic feedback to the operator, so that the operator can obtain the current state of the flexible vesicle portion being pressed according to the tactile and acoustic feedback, avoids the situation of insufficient pressing or excessive pressing, makes quantitative pipetting more intuitive, and improves the efficiency of quantitative pipetting.
  • the operator relaxes the pressing on the flexible vesicle part, and the elastic quantitative frame 62 can reset to the initial position from the quantitative position under the action of its own elastic force, and collide with the stopper 61 during the resetting process.
  • the operator can obtain the current reset state of the dosage feedback component 6 through tactile and acoustic feedback, which is convenient for subsequent repeated quantitative pipetting pressing.
  • the dosage feedback component 6 can be repeatedly reset during the operation of the flexible vesicle part, and give the operator tactile and acoustic feedback of quantitative and reset, which can facilitate the operator to accurately control the quantitative pipetting and reset, and improve the convenience of operation and the accuracy of pipetting quantitative.
  • the reagent tube 100 in the present embodiment can save the operation of the liquid transfer gun, and can directly and repeatedly quantitatively drip liquid.
  • the stopper 61 can generate interference with the elastic quantitative frame 62 during the movement of the elastic quantitative frame 62 to provide tactile and acoustic feedback.
  • the structure is simple, the cost is low, and it is easy to achieve batch production.
  • the quantitative position is located at the lower side of the initial position, and the stopper 61 is located between the initial position and the quantitative position along the up and down direction.
  • the stopper 61 located between the initial position and the quantitative position can interfere with the elastic quantitative frame 62, and emits acoustic and tactile feedback, so that the operator stops pressing, and the stroke stops.
  • the quantitative solution can drip through the liquid outlet 21, and the operator manually disengages from the flexible vesicle portion, so that the elastic quantitative frame 62 is reset under the action of its own elastic force, and the operator is fed back to the intuitive feedback in place through acoustic and tactile feedback.
  • the elastic quantitative frame 62 includes a reset spring 621 and a pressure rod 622.
  • the reset spring 621 is used to collide with The stopper 61 and the cantilever connection end of the reset spring 621 are connected to the inner tube wall of the tube body assembly 1; the pressure rod 622 is connected to the cantilever extension end of the reset spring 621 and is located on the side of the reset spring 621 facing the flexible vesicle part, and a pressing gap is formed between the flexible vesicle part and the pressure rod 622 located at the initial position.
  • the reagent tube 100 also includes a flexible rebound member 8 and a support member 7 arranged at the second end of the tube body assembly 1; the flexible rebound member 8 is arranged in the extrusion cavity, the upper side wall of the flexible rebound member 8 is connected to the inner side wall of the flexible vesicle portion, and the lower side wall of the flexible rebound member 8 is connected to the top wall of the support member 7.
  • the support member 7 is arranged at the second end of the tube body assembly 1; the flexible resilient member 8 is arranged in the extrusion cavity, the upper side wall of the flexible resilient member 8 is connected to the inner side wall of the flexible vesicle portion, and the lower side wall of the flexible resilient member 8 is connected to the top wall of the support member 7.
  • the reagent tube 100 has a simple structure, and the upper and lower sides of the flexible resilient member 8 are respectively connected to the flexible vesicle portion and the support member 7.
  • the flexible resilient member 8 can drive the flexible vesicle portion in the extruded state to quickly return to its original state, which is beneficial to shorten the time interval between two adjacent extrusions of the vesicle body, which is beneficial to improve the efficiency of the test personnel in pipetting, and can also avoid the volume of the extrusion cavity from changing compared to before extrusion, further ensuring the accuracy of quantitative pipetting.
  • an ultrasonic side wall breaking device 200 which includes a limit seat 210 and an ultrasonic generating mechanism 220.
  • the limit seat 210 is used for vertical placement of the reagent tube 100;
  • the ultrasonic generating mechanism 220 includes a transducer 230 and an ultrasonic block 240 connected to the transducer 230, and a contact head 250 is formed on the ultrasonic block 240 for laterally abutting against the side tube wall of the reagent tube 100.
  • the transducer 230 can generate ultrasonic waves, and the ultrasonic block 240 is connected to the transducer 230 and can conduct ultrasonic waves.
  • the limit seat 210 is used for the reagent tube 100 to be placed vertically so as to vertically support the reagent tube 100 on one side of the ultrasonic block 240.
  • a contact head 250 is formed on the ultrasonic block 240, and the contact head 250 abuts against the side wall of the reagent tube 100 from the side, so that the ultrasonic waves generated by the transducer 230 can be conducted to the side wall of the reagent tube 100 through the ultrasonic block 240 and the contact head 250, thereby performing ultrasonic vibration to break the wall of the liquid in the reagent tube 100, so that
  • the contact head 250 can abut against the side tube wall of the reagent tube 100 and ultrasonically break the wall of the sample in the reagent tube 100, which not only ensures stable contact, increases the ultrasonic conduction area, and improves the ultrasonic conduction effect, thereby greatly increasing the amount of nucleic acid released from the sample and improving the nucleic acid amplification effect, but also eliminates the need to transfer the sample from the reagent tube 100 to the microcentrifuge tube
  • Table 1 shows the ultrasonic wall breaking of influenza A virus using three wall breaking methods, namely, directly performing bottom ultrasound on the reagent tube 100, transferring the sample from the reagent tube 100 to a microcentrifuge tube for ultrasound, and using the ultrasonic side wall breaking device 200 of this embodiment to perform ultrasound on the reagent tube 100.
  • the wall breaking method of directly performing bottom ultrasound on the reagent tube 100 has poor ultrasonic conduction effect at the flexible vesicle part at the bottom of the reagent tube 100, resulting in poor detection.
  • the detection rate is only 75%, which affects the sample detection result; the detection rates of transferring the sample to a microcentrifuge tube for ultrasonication and using the ultrasonic side wall breaking device 200 in this embodiment to perform ultrasonication on the reagent tube 100 both reach 100%.
  • the wall breaking method of transferring the sample to a microcentrifuge tube for ultrasonication increases the operation steps of sample transfer, the wall breaking steps are relatively cumbersome, and the sample detection efficiency is low.
  • the contact head 250 is used to abut against the side tube wall of the reagent tube 100 to realize ultrasonic conduction from the side of the reagent tube 100.
  • the ultrasonic conduction effect is good, which improves the accuracy of the sample detection result, simplifies the operation steps, and improves the sample detection efficiency.
  • the limit seat 210 includes a seat body 2101 and a clamping assembly 2102.
  • the seat body 2101 is used for vertical placement of the reagent tube 100.
  • the transducer 230 and the clamping assembly 2102 are relatively spaced apart on the seat body 2101, and the clamping assembly 2102 is retractably arranged to cooperate with the contact head 250 to clamp the reagent tube 100.
  • the transducer 230 and the clamping assembly 2102 are both arranged on the seat body 2101, so that the contact head 250 and the clamping assembly 2102 are opposite to each other and arranged at intervals, and the reagent tube 100 is vertically placed between the contact head 250 and the clamping assembly 2102.
  • the clamping assembly 2102 can be extended and retracted relative to the contact head 250, so as to extend toward the direction close to the contact head 250 during ultrasonic wall breaking and cooperate with the contact head 250 to clamp the reagent tube 100 in the middle, so that the contact head 250 is pressed against the side tube wall of the reagent tube 100, which not only improves the reliability of ultrasonic wave conduction, but also improves the stability of the placement of the reagent tube 100.
  • the clamping assembly 2102 includes a mounting block 2103, a limiting column 2105 and an elastic member 2106.
  • the mounting block 2103 is arranged on the seat body 2101 and is spaced relative to the transducer 230.
  • a mounting hole 2104 corresponding to the contact head 250 is opened on the mounting block 2103.
  • One end of the limiting column 2105 extends into the mounting hole 2104 and can compress the elastic member 2106 in the mounting hole 2104.
  • the other end of the limiting column 2105 extends out of the mounting hole 2104 and is used to cooperate with the contact head 250 to clamp the reagent tube 100.
  • the transducer 230 and the mounting block 2103 are both arranged on the seat body 2101.
  • the side of the transducer 230 facing the mounting block 2103 is connected to the ultrasonic block 240.
  • the multiple contact heads 250 are all arranged on the side of the ultrasonic block 240 facing the mounting block 2103, and the multiple contact heads 250 are arranged at intervals along the width direction of the ultrasonic block 240.
  • the mounting block 2103 is provided with a plurality of mounting holes 2104, and the number of the mounting holes 2104 is consistent with the number of the contact heads 250 and is arranged one by one.
  • a limiting column 2105 and an elastic member 2106 are arranged in each mounting hole 2104, and the two ends of the elastic member 2106 are respectively connected to the limiting column 2105 and the elastic member 2106.
  • the column 2105 abuts against the inner wall of the mounting block 2103, and the limiting column 2105 can retract into the mounting hole 2104 and compress the elastic member 2106, so that the distance between the limiting column 2105 and the contact head 250 is increased, thereby facilitating the removal and placement of the reagent tube 100.
  • the elastic restoring force of the elastic member 2106 acts on the limiting column 2105, so that the limiting column 2105 extends toward the direction close to the contact head 250, and then cooperates with the contact head 250 to clamp the reagent tube 100 located in the middle, so that the contact head 250 is pressed against the side tube wall of the reagent tube 100, thereby ensuring the stability of ultrasonic transmission.
  • the present application also provides an ultrasonic side wall breaking method which is a flow chart of the first embodiment of the ultrasonic side wall breaking method of the present application, wherein the ultrasonic side wall breaking method is based on the ultrasonic side wall breaking device 200 according to the above, and the ultrasonic side wall breaking method includes:
  • Step S10 vertically placing the reagent tube 100 on the limiting seat 210, and making the side tube wall of the reagent tube 100 laterally abut against the contact head 250 on the ultrasonic block 240;
  • the reagent tube 100 containing the sample is placed on the limit seat 210 so that the limit seat 210 vertically supports the reagent tube 100 on one side of the ultrasonic block 240.
  • a contact head 250 is formed on the ultrasonic block 240, and the contact head 250 abuts against the side tube wall of the vertically placed reagent tube 100.
  • Step S20 controlling the transducer 230 to start emitting ultrasonic waves at a preset frequency, so that the ultrasonic waves are sequentially transmitted through the ultrasonic block 240 and the contact head 250 to the reagent tube 100;
  • the transducer 230 is controlled to turn on at a preset frequency, the preset frequency of the transducer 230 is 25KHz ⁇ 40KHz, the transducer 230 is turned on and emits ultrasonic waves, the ultrasonic block 240 is connected to the transducer 230 and is used to transmit the ultrasonic waves emitted by the transducer 230 to the contact head 250, so that the contact head 250 transmits the ultrasonic waves into the reagent tube 100 through the contact surface with the side wall of the reagent tube 100, and then ultrasonically breaks the wall of the sample in the reagent tube 100.
  • the preset frequency of the transducer 230 is 25KHz ⁇ 40KHz
  • the transducer 230 is turned on and emits ultrasonic waves
  • the ultrasonic block 240 is connected to the transducer 230 and is used to transmit the ultrasonic waves emitted by the transducer 230 to the contact head 250, so that the contact head 250 transmits the ultrasonic waves into the reagent tube
  • ultrasonic vibration is applied to the sample in the reagent tube 100.
  • the contact head 250 can abut against the side wall of the reagent tube 100 from the side of the reagent tube 100 and transmit the ultrasonic wave generated by the transducer 230.
  • step S10 includes:
  • Step S11 vertically placing a plurality of reagent tubes 100 in a plurality of placement positions in sequence along the width direction of the ultrasonic block 240, and making the side tube walls of the plurality of reagent tubes 100 correspondingly and laterally abutting against a plurality of contact heads 250 on the ultrasonic block 24030;
  • a plurality of contact heads 250 are arranged at intervals on the ultrasonic block 240 along the width direction of the ultrasonic block 240, and a placement position is formed on the limit seat 210 corresponding to the position of each contact head 250, and a plurality of reagent tubes 100 containing samples are vertically placed one by one in a plurality of placement positions, so that each contact head 250 is laterally abutted against the side tube wall of a reagent tube 100.
  • the transducer 230 is controlled to emit ultrasonic waves at a preset frequency.
  • the ultrasonic energy emitted by the transducer 230 is transmitted to the multiple contact heads 250 through the ultrasonic block 240, so that the multiple contact heads 250 can synchronously transmit the ultrasonic waves to the multiple reagent tubes 100, thereby achieving synchronous wall breaking of samples in the multiple reagent tubes 100, greatly improving the wall breaking efficiency.
  • step S10 includes:
  • Step S12 retracting the clamping assembly 2102 relative to the contact head 250 so that the distance between the clamping assembly 2102 and the contact head 250 increases;
  • the transducer 230 and the clamping assembly 2102 are arranged on the seat body 2101 at a relative interval, the ultrasonic block 240 is connected to the transducer 230, and the contact head 250 is arranged at one end of the ultrasonic block 240 facing the clamping assembly 2102.
  • the clamping assembly 2102 is retractable relative to the contact head 250.
  • the clamping assembly 2102 is retracted relative to the contact head 250 to make the clamping assembly 2102 move away from the contact head 250 and increase the distance between the clamping assembly 2102 and the contact head 250, thereby facilitating the placement of the reagent tube 100 between the contact head 250 and the clamping assembly 2102.
  • Step S13 vertically placing the reagent tube 100 on the seat body 2101, and making the side tube wall of the reagent tube 100 abut against the contact head 250 laterally, and the reagent tube 100 is located between the contact head 250 and the clamping assembly 2102;
  • the reagent tube 100 containing the sample is placed on the limiting seat 210 so that the limiting seat 210 vertically supports the reagent tube 100 between the contact head 250 and the clamping assembly 2102, and the contact head 250 abuts against the side tube wall of the vertically placed reagent tube 100.
  • Step S14 extending the clamping assembly 2102 relative to the contact head 250, so that the clamping assembly 2102 cooperates with the contact head 250 to clamp the reagent tube 100;
  • the clamping assembly 2102 is extended relative to the contact head 250 so that the clamping assembly 2102 is close to the contact head 250 and the distance between the clamping assembly 2102 and the contact head 250 is reduced.
  • the clamping assembly 2102 is extended to abut against the side tube wall of the reagent tube 100
  • the clamping assembly 2102 and the contact head 250 are respectively abutted against the two sides of the reagent tube 100, so that the clamping assembly 2102 can cooperate with the contact head 250 to clamp the reagent tube 100, ensuring that the reagent tube 100 always remains stable during the ultrasonic wall breaking process, which not only improves the reliability of ultrasonic wave conduction, but also improves the stability of the placement of the reagent tube 100.
  • first and second are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as “first” or “second” may explicitly or implicitly include at least one of the features.
  • “plurality” means at least two, such as two, three, etc., unless otherwise clearly and specifically defined.
  • the terms “installed”, “connected”, “connected”, “fixed” and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined.
  • installed installed”, “connected”, “connected”, “fixed” and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined.
  • the specific meanings of the above terms in this application can be understood according to specific circumstances.

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Abstract

L'invention concerne un tube de réactif (100), un dispositif de rupture de paroi latérale ultrasonore (200) et un procédé de rupture de paroi latérale ultrasonore. Ledit tube de réactif (100) comprend : un composant de corps de tube (1) à l'intérieur duquel est formée une cavité de réception (11) pour recevoir un liquide ; un goutteur (2), qui est disposé de manière amovible au niveau d'une première extrémité du composant de corps de tube (1) et forme une sortie de liquide (21) conçue pour être en communication avec la cavité de réception (11) ; un capuchon (3), qui est emmanché de manière amovible sur un côté périphérique externe du goutteur (2) et est utilisé pour bloquer la sortie de liquide (21) ; et un composant de compression (4) dont un espace de compression quantitatif (5) est formé entre lui (4) et le composant de corps de tube (1), et ledit composant de compression (4) est utilisé pour presser le composant de corps de tube (1), de telle sorte que le liquide dans la cavité de réception (11) s'écoule quantitativement à travers la sortie de liquide (21). L'espace de compression quantitative est formé entre le composant de corps de tube (1) et le composant de compression (4) ; le composant de compression (4) est pourvu d'un espace de déplacement quantitatif, et lorsque le composant de corps de tube (1) est pressé au moyen du composant de compression (4), le liquide dans la cavité de réception (11) peut s'écouler quantitativement à travers la sortie de liquide (21), de telle sorte que le tube de réactif effectue une distribution quantitative. Le dispositif de rupture de paroi latérale ultrasonore (200) comprend : une base de limitation (210), qui est utilisée pour le placement vertical d'un tube de réactif (100) ; et un mécanisme de génération d'ultrasons (220), qui comprend un transducteur (230) et un bloc ultrasonore (240) relié au transducteur (230). Une tête de contact (250) conçue pour venir latéralement en butée contre une paroi de tube latéral du tube de réactif (100) est formée sur le bloc ultrasonore (240).
PCT/CN2024/088990 2023-04-20 2024-04-20 Tube de réactif, dispositif de rupture de paroi latérale ultrasonore et procédé de rupture de paroi latérale ultrasonore Pending WO2024217580A1 (fr)

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CN202310430912.1A CN118807862A (zh) 2023-04-20 2023-04-20 用于检测设备的试剂管和检测设备
CN202320904032.9U CN220027057U (zh) 2023-04-20 2023-04-20 用于检测设备的试剂管和检测设备
CN202310430912.1 2023-04-20
CN202320904854.7 2023-04-20
CN202310429326.5 2023-04-20
CN202320909698.3U CN220194894U (zh) 2023-04-20 2023-04-20 用于检测设备的试剂管和检测设备
CN202320901967.1U CN219984727U (zh) 2023-04-20 2023-04-20 定量移液装置和样本检测设备
CN202320909698.3 2023-04-20
CN202310429326.5A CN118807861A (zh) 2023-04-20 2023-04-20 试剂管和检测设备
CN202320904854.7U CN220027058U (zh) 2023-04-20 2023-04-20 定量移液装置和样本检测设备
CN202320901967.1 2023-04-20
CN202320904032.9 2023-04-20
CN202321037612.9 2023-05-04
CN202321041867.2 2023-05-04
CN202321041302.4U CN220056819U (zh) 2023-05-04 2023-05-04 用于检测设备的试剂管和检测设备
CN202321037366.7U CN220027060U (zh) 2023-05-04 2023-05-04 用于试剂管的滴头组件、试剂管和检测设备
CN202321037366.7 2023-05-04
CN202321041302.4 2023-05-04
CN202321041867.2U CN220056820U (zh) 2023-05-04 2023-05-04 用于检测设备的试剂管和检测设备
CN202321037612.9U CN220027061U (zh) 2023-05-04 2023-05-04 用于检测设备的试剂管和检测设备
CN202311372389.8 2023-10-20
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CN202323102364.4 2023-11-16
CN202323102205.4U CN221371159U (zh) 2023-11-16 2023-11-16 用于检测设备的试剂管和检测设备
CN202323102205.4 2023-11-16
CN202323102349.XU CN221275781U (zh) 2023-11-16 2023-11-16 试剂管和检测设备
CN202323102364.4U CN221275782U (zh) 2023-11-16 2023-11-16 试剂管和检测设备
CN202323102349.X 2023-11-16
CN202323107003.9 2023-11-16
CN202323107003.9U CN221230604U (zh) 2023-11-16 2023-11-16 滴管

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