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WO2024263564A3 - Multi-chambered electroporation cartridge and methods of use - Google Patents

Multi-chambered electroporation cartridge and methods of use Download PDF

Info

Publication number
WO2024263564A3
WO2024263564A3 PCT/US2024/034474 US2024034474W WO2024263564A3 WO 2024263564 A3 WO2024263564 A3 WO 2024263564A3 US 2024034474 W US2024034474 W US 2024034474W WO 2024263564 A3 WO2024263564 A3 WO 2024263564A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
chamber
separate chambers
methods
electroporation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/034474
Other languages
French (fr)
Other versions
WO2024263564A2 (en
Inventor
Ludger Altrogge
Timo Gleissner
Frank MORRISSEY-WETTEY
Claudiu TODOR
Martin Stainer
Paul WAROK
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza Cologne GmbH
Lonza Walkersville Inc
Original Assignee
Lonza Cologne GmbH
Lonza Walkersville Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Cologne GmbH, Lonza Walkersville Inc filed Critical Lonza Cologne GmbH
Priority to CN202480018634.1A priority Critical patent/CN120882852A/en
Publication of WO2024263564A2 publication Critical patent/WO2024263564A2/en
Publication of WO2024263564A3 publication Critical patent/WO2024263564A3/en
Priority to IL323095A priority patent/IL323095A/en
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present disclosure provides systems, devices, and methods for electroporating at least one cell in a plurality of cells. A cartridge divided into a plurality of separate chambers is provided, where each chamber of the plurality of separate chambers is configured to hold a media comprising the at least one cell. Each chamber of the plurality of separate chambers is fluidly connected via a fluid exchange path, and are functionally separated by constrictions disposed within the fluid exchange path. Each chamber of the plurality of separate chambers includes electrodes for applying a pulsed electrical field to the at least one cell to achieve electroporation and transfection of the cell with nucleic acids. The constrictions disposed between each chamber prevents electric field interference between adjacent chambers during the electroporation procedures.
PCT/US2024/034474 2023-06-21 2024-06-18 Multi-chambered electroporation cartridge and methods of use Pending WO2024263564A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202480018634.1A CN120882852A (en) 2023-06-21 2024-06-18 Multi-chamber electroporation box and its usage method
IL323095A IL323095A (en) 2023-06-21 2025-09-02 Multi-chambered electroporation cartridge and methods of use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363509319P 2023-06-21 2023-06-21
US63/509,319 2023-06-21

Publications (2)

Publication Number Publication Date
WO2024263564A2 WO2024263564A2 (en) 2024-12-26
WO2024263564A3 true WO2024263564A3 (en) 2025-05-15

Family

ID=93936172

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/034474 Pending WO2024263564A2 (en) 2023-06-21 2024-06-18 Multi-chambered electroporation cartridge and methods of use

Country Status (3)

Country Link
CN (1) CN120882852A (en)
IL (1) IL323095A (en)
WO (1) WO2024263564A2 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9574245B2 (en) * 2011-07-25 2017-02-21 Qvella Corporation Methods and devices for electrical sample preparation
US20190062735A1 (en) * 2016-03-04 2019-02-28 Editas Medicine, Inc. Crispr-cpf1-related methods, compositions and components for cancer immunotherapy
US20200318104A1 (en) * 2015-02-22 2020-10-08 The Board Of Trustees Of The Leland Stanford Junior University Micro-screening Apparatus, Process, and Products
US10907178B2 (en) * 2017-09-30 2021-02-02 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems comprising flow-through electroporation devices
US20210123009A1 (en) * 2019-10-25 2021-04-29 Life Technologies Corporation Systems, methods, and devices for electroporation of cell-containing fluid
US20210139837A1 (en) * 2019-11-11 2021-05-13 Precigen, Inc. Electroporation apparatus and method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9574245B2 (en) * 2011-07-25 2017-02-21 Qvella Corporation Methods and devices for electrical sample preparation
US20200318104A1 (en) * 2015-02-22 2020-10-08 The Board Of Trustees Of The Leland Stanford Junior University Micro-screening Apparatus, Process, and Products
US20190062735A1 (en) * 2016-03-04 2019-02-28 Editas Medicine, Inc. Crispr-cpf1-related methods, compositions and components for cancer immunotherapy
US10907178B2 (en) * 2017-09-30 2021-02-02 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems comprising flow-through electroporation devices
US20210123009A1 (en) * 2019-10-25 2021-04-29 Life Technologies Corporation Systems, methods, and devices for electroporation of cell-containing fluid
US20210139837A1 (en) * 2019-11-11 2021-05-13 Precigen, Inc. Electroporation apparatus and method

Also Published As

Publication number Publication date
WO2024263564A2 (en) 2024-12-26
IL323095A (en) 2025-11-01
CN120882852A (en) 2025-10-31

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