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WO2024258379A1 - Procédé de transfert d'échantillons de sang et produit de transport pour celui-ci - Google Patents

Procédé de transfert d'échantillons de sang et produit de transport pour celui-ci Download PDF

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Publication number
WO2024258379A1
WO2024258379A1 PCT/TR2024/050486 TR2024050486W WO2024258379A1 WO 2024258379 A1 WO2024258379 A1 WO 2024258379A1 TR 2024050486 W TR2024050486 W TR 2024050486W WO 2024258379 A1 WO2024258379 A1 WO 2024258379A1
Authority
WO
WIPO (PCT)
Prior art keywords
blood
blood samples
transportation
blood sample
samples
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/TR2024/050486
Other languages
English (en)
Inventor
Haldun DOGAN
Bora ERGIN
Sena GJOTA ERGIN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Intergen Genetik Ve Nadir Hastaliklar Tani Arastirma Ve Uygulama Merkezi AS
Original Assignee
Intergen Genetik Ve Nadir Hastaliklar Tani Arastirma Ve Uygulama Merkezi AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from TR2023/006948 external-priority patent/TR2023006948A1/tr
Application filed by Intergen Genetik Ve Nadir Hastaliklar Tani Arastirma Ve Uygulama Merkezi AS filed Critical Intergen Genetik Ve Nadir Hastaliklar Tani Arastirma Ve Uygulama Merkezi AS
Publication of WO2024258379A1 publication Critical patent/WO2024258379A1/fr
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/128—Chemically defined matrices for immobilising, holding or storing living parts, e.g. alginate gels; Chemically altering living parts, e.g. by cross-linking

Definitions

  • the invention relates to a method that provides dry blood transfer in accordance with regulations so that blood samples can be transported and stored in solid form over international and intercity distances, and a transportation product performing this method.
  • Blood is the most commonly used biological sample for the field of molecular diagnostics. There are various regulations for transporting and storing blood samples over international and intercity distances:
  • IATA International Air Transport Association
  • Blood samples may also be subject to temperature controls, which may require special transport conditions. These conditions may include specific temperature ranges to ensure that blood samples are transported intact.
  • the vehicles used during the transport of blood samples may need to have special conditions.
  • vehicles carrying blood samples may need to have temperature controls or follow different safety protocols depending on where the blood samples will be transported to.
  • Dry blood transfer is a method in which blood is transported in solid form (i.e., without separation of serum or plasma). Dry blood transfer is a safe and effective method of transporting blood samples. In order for this method to be used, all equipment and materials must be properly prepared and used. In addition, dry blood transfer
  • Guthrie paper is a filter paper frequently used in international and intercity dry blood transfer. Using a special filtration technology, this paper separates the blood components such as serum and plasma and takes the form of a filter paper containing only the blood cells. Blood samples are applied to Guthrie paper by absorption, and the paper is labeled and properly packaged in accordance with certain rules and prepared for international and intercity transportation.
  • the use of Guthrie paper for international and intercity dry blood transfer ensures safe and effective transport of blood samples. This paper allows blood samples to be transported in a dry state, thus reducing the risk of samples leaking or spoiling. Furthermore, Guthrie paper ensures that blood samples are properly filtered and labeled, making it easy to accurately identify and track samples.
  • Guthrie paper is susceptible to contamination by blood samples on paper. Therefore, anything that comes into contact with the paper can cause the results to be misleading.
  • paper-based filters are often used so that dry blood can be transported and stored over long distances.
  • These paper-based products have disadvantages such as contamination problems in the transportation of dry blood samples over long distances, the inability of paper to have reliable components for long-term storage, the risk of contamination and the fact that it does not allow the transportation of large quantities of samples.
  • paper-based filters cannot ensure that dry blood samples are transported and stored in large quantities. 1 drop of liquid is considered to be approximately 30 microliters (one thousandth of a milliliter); in routine molecular diagnostic tests, approximately 100 - 200 microliters (3-6 drops) of blood sample are needed per sample. While paper-based filters can only store and transport blood samples up to these values, they cannot function when more blood samples are required. Additionally, if some absorption occurs in blood samples, such that paper-based products cannot be used, loss of sample amount occurs. Therefore, the efficiency of existing systems is low.
  • the present inventors put forward a method that makes it possible to transport and store blood samples in high quantities and over long distances for the relevant technical field and to eliminate other mentioned technical problems.
  • the present inventors argue that it is more appropriate to store and transport blood samples by performing absorption processes with chemical solutions, rather than storing and transporting them in absorbent paper-based products.
  • the present invention provides a product in which dry blood samples can be transported and stored with the use of said chemical solutions. Thanks to said product, it is possible to transfer a particularly high amount of blood samples. In addition, the risk of pollution and contamination, which is one of the technical disadvantages that may be encountered in paper-based products, is not possible in the product of the present invention.
  • the subject of the invention is related to a method for transporting and storing dry blood samples, and is described only with examples that will not create any limiting effect for a better understanding of the subject.
  • the method of the present invention is an alternative method to the paper-based filters that are frequently used in the art to transport and store blood samples in dry form.
  • blood samples are transported in dry form by forming chemical bonds with solutions.
  • the method of the present invention mainly comprises three main process steps:
  • 1 drop of liquid blood sample is approximately 30 microliters.
  • a liquid blood sample of approximately 100 to 200 microliters, that is, 3-6 drops, is needed per sample.
  • the development of a method that allows the transportation and storage of large quantities of blood samples is a need for the relevant technical field.
  • the addition of retaining powder and conversion solution (also called dispersion) as much as the amount of blood sample can make it possible to obtain large amounts of blood samples.
  • sodium polyacrylate compound is used as the retaining powder.
  • the sodium polyacrylate compound used as the retaining powder in this invention has the formula CsHyNaCh; and its molecular weight is 94.04 g/mol.
  • sodium polyacrylate compound is used as a retaining powder in the method of the present invention is that it is an anionic polyelectrolyte thanks to the negatively charged carboxylic groups it carries.
  • Sodium polyacrylate which comes into contact with the blood sample, increases the viscosity and forms a dense gel-shaped structure by elongating the chains attached to the anionic groups in its structure.
  • One of the major advantages of sodium polyacrylate is that the release rate of molecularly bound blood samples is very low compared to other similar retaining molecules.
  • a process is carried out between the retaining powder and the liquid blood sample with a value between 0.3 and 0.6 g/ml.
  • This ratio is critical because the increase in the amount of sodium polyacrylate compound in the mixture causes an increase in the undesired gelling rate. If a small amount of sodium polyacrylate is used, it is caused that the targeted amount of blood sample cannot be stored.
  • a period of at least 30 minutes is needed after the retaining powder and the liquid blood sample are combined together. This is the time required for the targeted interactions between the retaining powder and liquid blood to occur.
  • the conversion solution contains at least one magnesium-containing component.
  • the conversion solution is a solution of MgCl2 at a concentration of 1 M.
  • a process is carried out between the conversion solution and the liquid blood sample with a value between 0.3 and 0.6 ml/ml.
  • a conversion solution within the mentioned value ranges can degrade the gel structure in targeted amounts and make it possible to obtain a free liquid blood sample.
  • the invention provides a transportation product that allows the implementation of the method characterized.
  • the transportation and storage of dry blood samples was usually carried out on paper-based products.
  • structures that can be called "cassettes” are used as transportation products.
  • the transportation product of the present invention comprises at least one compartment.
  • the said compartment is the part where the retaining powder and blood sample used in the method for storage and transportation activities are trapped in the first place. If preferred, the number of compartments is more than one. Naturally, with the increase in the number of compartments, the amount of blood that can be stored and transported may increase.
  • the transportation product of the present invention may comprise more than one independent compartment. In this way, it is possible to store and transport blood samples obtained from the same or different people in independent compartments.
  • the upper part of the transportation product of the present invention is open so that samples can be placed into the compartment part. Via the opening, the samples are placed into the transportation product and it comprises at least one lid to cover the upper part and to prevent the samples from spilling, scattering or mixing.
  • Said cover is preferably of the same size as a lower part containing a compartment.
  • the inside of the lid is hollow and is positioned above the lower part, allowing these compartments to be covered.
  • the transportation product of the present invention is of a simple structure; it comprises a lower part and at least one compartment in the lower part where blood samples and retaining powder are added for storage and transportation activities, and at least one lid that allows the compartments to be covered from the upper part, depending on the shape of the lower part.
  • the transportation product of the present invention is made of non-paper-based materials as in the art. It is preferably made of polymer materials or composite materials containing polymer material. In this way, technical problems, pollution, contamination and storage problems arising from the components of paper-based products are prevented. At least 10 mL of blood sample can be transported and stored with the transportation product of the present invention.
  • the transportation product of the present invention is preferred, it can be produced easily and quickly by means of three-dimensional printer technology.
  • the elements within the transportation product of the present invention can be produced in different sizes or colors. If preferred, features such as text, code, name can be added to the elements.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention concerne un procédé qui assure un transfert de sang sec conformément à des réglementations de telle sorte que des échantillons de sang peuvent être transportés sur des distances internationales et interurbaines, et un produit de transport mettant en oeuvre ce procédé.
PCT/TR2024/050486 2023-06-13 2024-05-15 Procédé de transfert d'échantillons de sang et produit de transport pour celui-ci Pending WO2024258379A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2023/006948 TR2023006948A1 (tr) 2023-06-13 Kan numuneleri̇ni̇n transferi̇ i̇çi̇n bi̇r yöntem ve bunu sağlayan bi̇r taşima ürünü
TR2023006948 2023-06-13

Publications (1)

Publication Number Publication Date
WO2024258379A1 true WO2024258379A1 (fr) 2024-12-19

Family

ID=93852484

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/TR2024/050486 Pending WO2024258379A1 (fr) 2023-06-13 2024-05-15 Procédé de transfert d'échantillons de sang et produit de transport pour celui-ci

Country Status (1)

Country Link
WO (1) WO2024258379A1 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4295987A (en) * 1979-12-26 1981-10-20 The Procter & Gamble Company Cross-linked sodium polyacrylate absorbent
EP0676968A1 (fr) * 1992-12-30 1995-10-18 Chemische Fabrik Stockhausen GmbH Polymeres pulverulents absorbant en charge les liquides aqueux et le sang, procede pour leur preparation et utilisation dans des produits textiles pour l'hygiene corporelle

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4295987A (en) * 1979-12-26 1981-10-20 The Procter & Gamble Company Cross-linked sodium polyacrylate absorbent
EP0676968A1 (fr) * 1992-12-30 1995-10-18 Chemische Fabrik Stockhausen GmbH Polymeres pulverulents absorbant en charge les liquides aqueux et le sang, procede pour leur preparation et utilisation dans des produits textiles pour l'hygiene corporelle

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BHAPSE ONKAR: "Phase Change Materials: An Overview", MEDIUM, 22 June 2022 (2022-06-22), XP093255559, Retrieved from the Internet <URL:https://medium.com/@onkar.bhapse19/phase-change-materials-anoverview-883be147adff> *
JIANG DEZHI; D'ORAZIO ANNUNZIATA; ALRABAIAH HUSSAM; FAGIRY MORAM A.; SAJADI S. MOHAMMAD; LI ZHIXIONG: "Molecular dynamics approach to study the effects of MgCl2 salt atomic ratio on the phase transition phenomenon in the phase change materials", JOURNAL OF ENERGY STORAGE, ELSEVIER BV, NL, vol. 46, 24 December 2021 (2021-12-24), NL , XP086940373, ISSN: 2352-152X, DOI: 10.1016/j.est.2021.103860 *
XU TING, ZHU WENXIANG, SUN JIAN: "Structural Modifications of Sodium Polyacrylate-Polyacrylamide to Enhance Its Water Absorption Rate", COATINGS, M D P I AG, CH, vol. 12, no. 9, CH , pages 1234, XP093255556, ISSN: 2079-6412, DOI: 10.3390/coatings12091234 *

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