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WO2024249849A3 - Scalable platform for single cell proteomics via digitized detection of proteins and their proteoforms - Google Patents

Scalable platform for single cell proteomics via digitized detection of proteins and their proteoforms Download PDF

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Publication number
WO2024249849A3
WO2024249849A3 PCT/US2024/031987 US2024031987W WO2024249849A3 WO 2024249849 A3 WO2024249849 A3 WO 2024249849A3 US 2024031987 W US2024031987 W US 2024031987W WO 2024249849 A3 WO2024249849 A3 WO 2024249849A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
proteoform
liquid droplet
proteoforms
single cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/031987
Other languages
French (fr)
Other versions
WO2024249849A2 (en
Inventor
Neil L. Kelleher
Jared Otto KAFADER
Pei SU
Michael AR HOLLAS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Northwestern University
Original Assignee
Northwestern University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Northwestern University filed Critical Northwestern University
Publication of WO2024249849A2 publication Critical patent/WO2024249849A2/en
Publication of WO2024249849A3 publication Critical patent/WO2024249849A3/en
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

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Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/0027—Methods for using particle spectrometers
    • H01J49/0036—Step by step routines describing the handling of the data generated during a measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Disclosed herein are systems and methods for single-cell proteoform imaging mass spectrometry. The method includes moving a liquid droplet comprising a solvent across a substrate having disaggregated cells thereon, sampling the moving liquid droplet with an ionization source, wherein sampling the moving liquid droplet comprises detecting a discrete cell extraction profile comprising a multiplicity of proteoform ions when the moving liquid droplet contacts a disaggregated cell on the substrate, aggregating a mass-domain spectrum from a plurality of discreate cell extraction profiles; assigning a single cell proteoform from the aggregated mass-domain spectrum; and identifying one or more proteoforms from the assigned the single cell proteoform.
PCT/US2024/031987 2023-05-31 2024-05-31 Scalable platform for single cell proteomics via digitized detection of proteins and their proteoforms Pending WO2024249849A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363505404P 2023-05-31 2023-05-31
US63/505,404 2023-05-31

Publications (2)

Publication Number Publication Date
WO2024249849A2 WO2024249849A2 (en) 2024-12-05
WO2024249849A3 true WO2024249849A3 (en) 2025-02-06

Family

ID=93658620

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/031987 Pending WO2024249849A2 (en) 2023-05-31 2024-05-31 Scalable platform for single cell proteomics via digitized detection of proteins and their proteoforms

Country Status (1)

Country Link
WO (1) WO2024249849A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10151758B2 (en) * 2016-01-14 2018-12-11 Thermo Finnigan Llc Methods for top-down multiplexed mass spectral analysis of mixtures of proteins or polypeptides
US10665439B2 (en) * 2017-01-26 2020-05-26 Protein Metrics Inc. Methods and apparatuses for determining the intact mass of large molecules from mass spectrographic data
US20220043001A1 (en) * 2014-04-10 2022-02-10 Yale University Methods and compositions for detecting misfolded proteins
WO2023076583A1 (en) * 2021-10-29 2023-05-04 Northwestern University Automatic ion population control for charge detection mass spectrometry

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220043001A1 (en) * 2014-04-10 2022-02-10 Yale University Methods and compositions for detecting misfolded proteins
US10151758B2 (en) * 2016-01-14 2018-12-11 Thermo Finnigan Llc Methods for top-down multiplexed mass spectral analysis of mixtures of proteins or polypeptides
US10665439B2 (en) * 2017-01-26 2020-05-26 Protein Metrics Inc. Methods and apparatuses for determining the intact mass of large molecules from mass spectrographic data
WO2023076583A1 (en) * 2021-10-29 2023-05-04 Northwestern University Automatic ion population control for charge detection mass spectrometry

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SU PEI, HOLLAS MICHAEL A. R., BUTUN FATMA AYALOGLU, KANCHUSTAMBHAM VIJAYA LAKSHMI, RUBAKHIN STANISLAV, RAMANI NAMRATA, GREER JOSEP: "Single Cell Analysis of Proteoforms", JOURNAL OF PROTEOME RESEARCH, AMERICAN CHEMICAL SOCIETY, vol. 23, no. 6, 7 June 2024 (2024-06-07), pages 1883 - 1893, XP093277502, ISSN: 1535-3893, DOI: 10.1021/acs.jproteome.4c00075 *

Also Published As

Publication number Publication date
WO2024249849A2 (en) 2024-12-05

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