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WO2024134252A1 - Substance de type "gragel" avec une combinaison de chélate activateur, de gel polymère et de granules plastiques pour la séparation du sérum - Google Patents

Substance de type "gragel" avec une combinaison de chélate activateur, de gel polymère et de granules plastiques pour la séparation du sérum Download PDF

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Publication number
WO2024134252A1
WO2024134252A1 PCT/IB2022/062554 IB2022062554W WO2024134252A1 WO 2024134252 A1 WO2024134252 A1 WO 2024134252A1 IB 2022062554 W IB2022062554 W IB 2022062554W WO 2024134252 A1 WO2024134252 A1 WO 2024134252A1
Authority
WO
WIPO (PCT)
Prior art keywords
gel
polymer gel
density
serum
plastic granule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2022/062554
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English (en)
Inventor
Abbas JAFARI SOLTANI
Mahsa JAFARI SOLTANI
Roya SHAVAKH
Mahnoosh JAFARI SOLTANI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to PCT/IB2022/062554 priority Critical patent/WO2024134252A1/fr
Publication of WO2024134252A1 publication Critical patent/WO2024134252A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2300/00—Characterised by the use of unspecified polymers
    • C08J2300/14—Water soluble or water swellable polymers, e.g. aqueous gels
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J3/00—Processes of treating or compounding macromolecular substances
    • C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
    • C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
    • C08J3/075—Macromolecular gels

Definitions

  • a type of gragel with a density difference compared to gel and whole blood is placed on the gel and forms a strong layer that prevents it from hitting or even being sucked by the nozzle and as a result the nozzle of the device is not contaminated.
  • the filler that is added to increase the density of the polymer for separation one of its disadvantages is that in the long-term storage of the upper surface of the gel inside the tube, which is in contact with air, it is separated from the silica filler during the wet out process and during centrifugation. Polymer materials that have a lower density than serum float on the serum and interfere in the process of testing during sampling.
  • polyurethane polymer gel has been used for long-term storage because the final density of the composition is about 1.040 -1.045gr/cm3 obtained without the use of phyllosilicate materials and having a homogeneous density, does not release any materials during the separation process.
  • the tubes containing the agents that separate and activate the clot gel is used for the agent between serum and blood components after centrifugation, then the tube is placed on the autoanalyzer to perform biochemical tests. In their composition, they are separated during centrifugation and placed on the serum, and sometimes the nozzle of the biochemistry device collides with the top layer of the gel during serum sampling, or the surface layer of the gel is sucked and causes the nozzle of the autoanalyzer to be contaminated. It prevents correct sampling.
  • the density of serum is gr/cm3 (1.02-1.03) and the density of blood cells is gr/cm3 (1.095-1.09).
  • the density required for normal separation is between 1.035-1.085 gr/cm3.
  • the density of serum is gr/cm3 (1.02-1.03) and the density of blood cells is gr/cm3 (1.095-1.09).
  • the density required for separation is usually between 1.035-1.085 gr/cm3
  • plastic granules are poured into the tube and then gel is injected in the amount of usually 0.7 gr by the gel injection device and then it is sprayed with clot activating substances on the inner wall of the tube.
  • the filler that is added to increase the density of the polymer for separation one of its disadvantages is that in the long-term storage of the upper surface of the gel inside the tube, which is in contact with air, it is separated from the silica filler during the wet out process and during centrifugation. Polymer materials that have a lower density than serum float on the serum and interfere in the process of testing during sampling.
  • polyurethane polymer gel has been used for long-term storage because the final density of the composition is about 1.040 -1.045gr/cm3 obtained without the use of phyllosilicate materials and having a homogeneous density, does not release any materials during the separation process.
  • the use of plastic granules placed at the bottom of the tube and under the gel after centrifugation, due to the difference in density, the plastic granules are placed on the upper surface of the gel and form a strong layer, and when sampling the serum, the possibility of the nozzle hitting the biochemical analyzer device prevents the contamination of the nozzle with a soft gel layer.
  • nano-silica materials with dimensions of 50-100 nanometers are used to accelerate the clotting process along with polyethylene glycol copolymer surfactant materials in order to facilitate the clotting process and non-adhesion of blood materials and components to the tube wall by spraying the inner tube wall. is placed.
  • Gragel with a combination of activator chelate, polymer gel and plastic granule for serum separation, polymer gel and plastic granule with a density difference mechanism for serum separation which consists of the following components: -Nano substances that activate the blood clot, -Surfactant substances to prevent proteins and blood cells from sticking to the inside of the tube wall, -Polymer gel and serum separating granule, which are placed together at the bottom of the tube, -Activator chelate, -Polymer gel, -Plastic granule, -Nano silica, - Polyethylene glycol copolymer surfactant material
  • One of the advantages of this invention is the use of a polymer gel with a homogeneous density, which, unlike previous inventions, maintains its homogeneity over a long period of storage and does not release substances during use.
  • Another advantage of this invention is the use of separating granule, which forms a strong top layer and prevents contamination of the nozzle head of the analyzer during sampling, and this feature is unique.
  • D DBTDL catalyst (C32H64O4SN)
  • polymer gel In this invention, about 0.7 grams of polymer gel has been injected into the bottom of the tube, which is made of polyurethane.
  • This polymer is a combination of the polyol polymer group, usually polyethylene glycol with a molecular weight of 500 to 1000 or polypropylene glycol with a molecular weight of 1000 to 2000 polymethyl diphenyl diisocyanate and toluene diisocyanate are synthesized in the presence of DBTDL catalyst.
  • polyol composition with a ratio of 5 PMDI isocyanate composition with a ratio of 0.5
  • the density of serum is gr/cm3 (1.02-1.03) and the density of blood cells is gr/cm3 (1.095-1.09).
  • the density required for separation is usually between 1.035-1.085 gr/cm3
  • plastic granules are poured into the tube and then gel is injected in the amount of usually 0.7 gr by the gel injection device and then it is sprayed with clot activating substances on the inner wall of the tube.
  • nano-silica materials with dimensions of 50-100 nanometers are used to accelerate the clotting process along with polyethylene glycol copolymer surfactant materials in order to facilitate the clotting process and non-adhesion of blood materials and components to the tube wall by spraying the inner tube wall. is placed.
  • the application of the claimed invention is used in laboratory and hospital centers to store and separate serum for biochemistry, hormone and serology tests.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La substance de type "Gragel" avec la combinaison d'activateur chélate, de gel polymère et de granules plastiques est utilisée pour séparer le sérum, dans le domaine de la collecte et de la séparation du sérum à partir du sang total. Dans la présente invention, un gel polymère, qui a une masse volumique de 1,04-1,045 g/cm3 et conserve ses propriétés initiales pendant une longue période de temps, et des granules de séparation avec un mécanisme de différence de masse volumique sont utilisés, et l'objectif de la présente invention est de fabriquer un gel polymère qui a une masse volumique qui est uniforme et homogène, qui ne sépare pas en particules pendant la centrifugation, ainsi que les granules ayant une différence de densité par comparaison avec le gel et le sang total sur le gel, qui forme une couche résistante et l'empêche d'être frappé ou même aspiré par la buse et, par conséquent,protège de la contamination de la buse de dispositif.
PCT/IB2022/062554 2022-12-20 2022-12-20 Substance de type "gragel" avec une combinaison de chélate activateur, de gel polymère et de granules plastiques pour la séparation du sérum Ceased WO2024134252A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/IB2022/062554 WO2024134252A1 (fr) 2022-12-20 2022-12-20 Substance de type "gragel" avec une combinaison de chélate activateur, de gel polymère et de granules plastiques pour la séparation du sérum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2022/062554 WO2024134252A1 (fr) 2022-12-20 2022-12-20 Substance de type "gragel" avec une combinaison de chélate activateur, de gel polymère et de granules plastiques pour la séparation du sérum

Publications (1)

Publication Number Publication Date
WO2024134252A1 true WO2024134252A1 (fr) 2024-06-27

Family

ID=91587851

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2022/062554 Ceased WO2024134252A1 (fr) 2022-12-20 2022-12-20 Substance de type "gragel" avec une combinaison de chélate activateur, de gel polymère et de granules plastiques pour la séparation du sérum

Country Status (1)

Country Link
WO (1) WO2024134252A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110250105A1 (en) * 2008-11-07 2011-10-13 Kunihiro Suto Blood serum or blood plasma separating material and blood-collecting tube using same
JP2018169214A (ja) * 2017-03-29 2018-11-01 東洋インキＳｃホールディングス株式会社 血液分離用組成物
KR20210069505A (ko) * 2019-12-03 2021-06-11 가톨릭관동대학교산학협력단 혈액응고촉진제가 도포된 초소형 채혈튜브

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110250105A1 (en) * 2008-11-07 2011-10-13 Kunihiro Suto Blood serum or blood plasma separating material and blood-collecting tube using same
JP2018169214A (ja) * 2017-03-29 2018-11-01 東洋インキＳｃホールディングス株式会社 血液分離用組成物
KR20210069505A (ko) * 2019-12-03 2021-06-11 가톨릭관동대학교산학협력단 혈액응고촉진제가 도포된 초소형 채혈튜브

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BABAKHANI, B. ET AL.: "A new formulation for polymeric separator gels for potential use in blood serum separator tubes", PROGRESS IN RUBBER PLASTICS AND RECYCLING TECHNOLOGY, vol. 34, no. 1, 2018 - 4 September 2018 (2018-09-04), pages 35 - 53, XP009545898, DOI: 10.1177/147776061803400103 *

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