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WO2024128651A1 - Procédé de production de collagène à l'aide d'un procédé de broyage humide - Google Patents

Procédé de production de collagène à l'aide d'un procédé de broyage humide Download PDF

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Publication number
WO2024128651A1
WO2024128651A1 PCT/KR2023/019719 KR2023019719W WO2024128651A1 WO 2024128651 A1 WO2024128651 A1 WO 2024128651A1 KR 2023019719 W KR2023019719 W KR 2023019719W WO 2024128651 A1 WO2024128651 A1 WO 2024128651A1
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WO
WIPO (PCT)
Prior art keywords
minutes
extract
cherry
collagen
grinding
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Ceased
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PCT/KR2023/019719
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English (en)
Korean (ko)
Inventor
박민선
유정진
배수지
임지영
강승현
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Cosmax Inc
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Cosmax Inc
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Filing date
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Publication of WO2024128651A1 publication Critical patent/WO2024128651A1/fr
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Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
    • A61K8/4913—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00—Function of food ingredients
    • A23V2200/30—Foods, ingredients or supplements having a functional effect on health
    • A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95

Definitions

  • the present invention relates to a method for producing collagen or producing an extract using wet grinding, and the use of the collagen or extract produced by the above method to improve skin wrinkles and promote elasticity.
  • Collagen is a major matrix protein produced by fibroblasts of the skin and exists in the extracellular matrix. Its important functions include mechanical rigidity of the skin, connective tissue resistance and tissue adhesion, support for cell adhesion, and cell division and differentiation (organism). It is known to induce growth or wound healing. This collagen decreases due to age and photoaging caused by ultraviolet irradiation, and this is known to be closely related to the formation of wrinkles in the skin. In addition, as extensive research on skin aging has developed in recent years, the important functions of collagen in the skin have been revealed.
  • retinoic acid retinoic acid
  • TGF transforming growth factor
  • protein derived from animal placenta protein derived from animal placenta
  • betulinic acid protein derived from animal placenta
  • chlorella extract etc.
  • the amount of the active ingredients is limited due to safety issues such as irritation and redness when applied to the skin, or the effect is so minimal that the effect of improving skin function by promoting collagen synthesis in the skin cannot be expected.
  • One aspect includes 1) grinding a raw material containing collagen using wet grinding (aqua grinding); and obtaining an extract or a fraction thereof from the pulverized raw material.
  • Another aspect provides a cosmetic composition for improving skin wrinkles or improving elasticity, comprising as an active ingredient one or more extracts or fractions thereof selected from cherry and cherry.
  • Another aspect provides a food composition for improving skin wrinkles or improving elasticity, comprising as an active ingredient one or more extracts or fractions thereof selected from cherry and cherry.
  • compositions for topical skin application for improving skin wrinkles or improving elasticity comprising as an active ingredient one or more extracts selected from cherry and cherry or a fraction thereof.
  • Another aspect includes 1) grinding a raw material containing collagen using wet grinding (aqua grinding); 2) Obtaining an extract or fraction thereof from the pulverized raw material; and 3) isolating collagen from the extract or fraction thereof.
  • One aspect includes 1) grinding a raw material containing collagen using wet grinding (aqua grinding); and 2) obtaining an extract or a fraction thereof from the pulverized raw material.
  • wet grinding in this specification refers to grinding in water or an organic solvent, and the presence of liquid improves the energy efficiency of grinding and reduces the agglomeration and caking of powder, making it easier to dry grind. Compared to other products, it has the advantage of being able to be more finely pulverized.
  • the step of grinding using wet grinding may be performed for 1 minute to 30 minutes, specifically 1 minute to 30 minutes, 1 minute to 25 minutes, 1 minute to 20 minutes, 1 minute to 17 minutes, and 1 minute. to 15 minutes, 1 minute to 10 minutes, 1 minute to 8 minutes, 1 minute to 6 minutes, 1 minute to 5 minutes, 2 minutes to 30 minutes, 2 minutes to 25 minutes, 2 minutes to 20 minutes, 2 minutes to 17 minutes minutes, 2 minutes to 15 minutes, 2 minutes to 10 minutes, 2 minutes to 8 minutes, 2 minutes to 6 minutes, 2 minutes to 5 minutes, 3 minutes to 30 minutes, 3 minutes to 25 minutes, 3 minutes to 20 minutes, 3 minutes to 17 minutes, 3 minutes to 15 minutes, 3 minutes to 10 minutes, 3 minutes to 8 minutes, 3 minutes to 6 minutes, 3 minutes to 5 minutes, 4 minutes to 30 minutes, 4 minutes to 25 minutes, 4 minutes to 20 minutes, 4 minutes to 17 minutes, 4 minutes to 15 minutes, 3 minutes to 10 minutes, 3 minutes to 8 minutes, 3 minutes to 6 minutes, 3 minutes to 5 minutes, 4 minutes to 30 minutes, 4 minutes to 25 minutes, 4 minutes to 20
  • the step of grinding using wet grinding may be performed under conditions of 50 °C or less, specifically 50 °C or less, 45 °C or less, 40 °C or less, 35 °C or less, and more specifically, 15 °C or less. to 50°C, 15 to 45°C, 15 to 40°C, 15 to 35°C, 20 to 50°C, 20 to 45°C, 20 to 40°C, 20 to 35°C, 25 to 50°C, 25 to 45°C, 25°C It may be carried out under conditions of 40°C to 40°C, 25 to 35°C, 30 to 50°C, 30 to 45°C, 30 to 40°C, or 30 to 35°C.
  • the step of grinding using wet grinding may be performed under conditions of 1000 to 10000 rpm, specifically 1000 to 10000 rpm, 1000 to 9000 rpm, 1000 to 8000 rpm, 1000 to 7000 rpm, 1000 to 6000 rpm, 1000 to 1000 rpm.
  • heat in the case of a wet grinding process, heat can be generated up to about 33 °C, which means that heat is only generated up to a significantly lower temperature than the existing dry grinding process.
  • Raw materials containing the collagen include cherry (Prunus Avium), cherry (Prunus Tomentosa), avocado (Persea americana), olive (Olea europaea), carrot (Daucus carota), bean (Glycine max), tomato (Solanum lycopersicum), and blue It may include one or more selected from the group consisting of berries (Vaccinium spp.), and may specifically be cherries or cherry.
  • cherry in this specification refers to the fruit of the cherry tree (Prunus Tomentosa), and is also called 'cherry'.
  • the cherry tree is a deciduous broad-leaved shrub native to North China, China, and is cultivated in the central and northern regions of Korea, reaching a height of approximately 1-3 m.
  • the fruit of the cherry tree is a ball-shaped drupe that ripens red around June.
  • the term “cherry” in this specification refers to the fruit of Prunus Avium (Sweet Cherry).
  • the fruit of the sheep cherry tree is round or oval-shaped, about 2.5 cm in diameter, ripens to yellow-red in June and July, and has a sweet taste.
  • the method may be a method for producing an extract or fraction thereof with a collagen component or increased collagen content, and specifically, a method for producing an extract or fraction thereof of cherries and/or cherry with an increased collagen component or collagen content. You can.
  • the cherry or cherry may be obtained from a natural, hybrid, or mutated plant of the corresponding plant, or may be obtained from plant tissue culture. Specifically, the cherry or cherry may be a dried raw material.
  • extract in this specification refers to the result, such as a liquid component obtained by immersing the desired material in various solvents and then extracting it at room temperature or at a heated state for a certain period of time, and a solid component obtained by removing the solvent from the liquid component. It can mean.
  • extracts of all formulations that can be formed using the extract itself and the extract such as dilutions of the results, concentrates, crude preparations, purifications, or mixtures thereof. You can.
  • the extraction solvent for the extract may be a protic polar solvent or an aprotic polar and non-polar solvent.
  • the protic polar solvent may be water, methanol, ethanol, propanol, isopropanol, or butanol.
  • the aprotic polar solvent may be dichloromethane, tetrahydrofuran, ethyl acetate, acetonitrile, dimethylformamide, dimethyl sulfoxide, acetone, 2-butanone, or hexamethylphosphoramide.
  • the non-polar solvent may be pentane, hexane, chloroform, or diethyl ether.
  • the non-polar solvent excludes benzene.
  • the solvent may be a C1-C6 alcohol, a C3-C10 ester, such as a C3-C10 acetate, a C3-C10 ketone, a C1-C6 unsubstituted or halogenated hydrocarbon, a C2-C10 ring ether, or mixtures thereof. or it may be a mixture of one or more of the above solvents and water.
  • the hydrocarbon may be an alkane, alkene, or alkyne.
  • the extract is water; C1 to C4 lower alcohols such as methanol, ethanol, propanol, and butanol; Polyhydric alcohols such as glycerine, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, bezene, hexane, diethyl ether, and dichloromethane. It may be extracted with one or more solvents selected from the group, and specifically, it may be extracted using a mixture of water and ethanol as the solvent. More specifically, the extraction solvent may be water (distilled water, purified water, etc.).
  • the extract may be extracted by one or more methods selected from the group consisting of reduced pressure high temperature extraction, boiling water extraction, reflux extraction, hot water extraction, cold immersion extraction, low temperature extraction, room temperature extraction, ultrasonic extraction, steam extraction, and fractional extraction, specifically low temperature extraction. It could be extraction.
  • fraction refers to a result obtained by performing fractionation to separate a specific component or specific group of components from a mixture containing various components.
  • the fractionation method for obtaining the above fraction is not particularly limited and may be performed according to methods commonly used in the art.
  • Non-limiting examples of the fractionation method include fractionation performed by treating various solvents, ultrafiltration fractionation performed by passing through an ultrafiltration membrane with a certain molecular weight cut-off value, and various chromatography methods (size, charge, hydrophobicity or There are chromatographic fractionation methods (designed for separation based on affinity), and combinations thereof.
  • the type of fractionation solvent used to obtain the fraction is not particularly limited, and any solvent known in the art can be used.
  • the fractionating solvent is water; C1 to C4 lower alcohols such as methanol, ethanol, propanol, and butanol; Polyhydric alcohols such as glycerine, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, bezene, hexane, diethyl ether, and dichloromethane. It may contain one or more solvents selected from the group.
  • collagen in this specification is a main component of connective tissue and is mainly found in bones and skin, but is also distributed throughout our body, including joints, membranes of each organ, and hair. It is the most abundant protein in mammals, accounting for 25-35% of the total protein content, and is a light protein also called 'collagen'.
  • the collagen may be cherry collagen or cherry collagen obtained from cherries or cherry trees.
  • energy consumption or carbon dioxide emissions may be reduced by more than 5% compared to a method using dry grinding instead of wet grinding. Specifically, specifically, 5% or more, 8% or more, 10% or more, 15% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, 90 It may be reduced by more than %, more than 99%, or 100%. More specifically 5% to 100%, 5% to 90%, 5% to 80%, 5% to 70%, 5% to 60%, 5% to 50%, 5% to 40%, 5% to 30%.
  • 5% to 20% 5% to 10%, 10% to 100%, 10% to 90%, 10% to 80%, 10% to 70%, 10% to 60%, 10% to 50%, 10 % to 40%, 10% to 30%, 10% to 20%, 30% to 100%, 30% to 90%, 30% to 80%, 30% to 70%, 30% to 60%, 30% to 30% 50%, 30% to 40%, 50% to 100%, 50% to 90%, 50% to 80%, 50% to 70%, 50% to 60%, 60% to 100%, 60% to 90% , may be reduced by 60% to 80%, or 60% to 70%.
  • the collagen content of the extract or fraction thereof prepared by the above method may be increased by 5% or more compared to the extract or fraction thereof prepared by a method using dry grinding instead of wet grinding. Specifically, specifically, 5% or more, 8% or more, 10% or more, 15% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, 90 It may be an increase of more than %, more than 99%, or 100%. More specifically 5% to 100%, 5% to 90%, 5% to 80%, 5% to 70%, 5% to 60%, 5% to 50%, 5% to 40%, 5% to 30%.
  • the content of one or more of hydroxyproline, glycine, and proline in the extract or fraction thereof prepared by the above method is 5% lower than that of the extract or fraction thereof prepared by a method using dry grinding instead of wet grinding. It may have increased. Specifically, specifically, 5% or more, 8% or more, 10% or more, 15% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, 90 It may be an increase of more than %, more than 99%, or 100%. More specifically 5% to 100%, 5% to 90%, 5% to 80%, 5% to 70%, 5% to 60%, 5% to 50%, 5% to 40%, 5% to 30%.
  • the method used a wet grinding process to obtain collagen from cherry or cherry raw material.
  • a wet grinding process to obtain collagen from cherry or cherry raw material.
  • energy consumption and heat generation can be reduced through the wet grinding process, and temperature-sensitive bioactive substances can be extracted more efficiently.
  • Another aspect is to provide a cosmetic composition for improving skin wrinkles or improving elasticity, comprising as an active ingredient one or more extracts or fractions thereof selected from cherry and cherry.
  • a cosmetic composition for improving skin wrinkles or improving elasticity comprising as an active ingredient one or more extracts or fractions thereof selected from cherry and cherry.
  • extracts or fractions thereof selected from cherry and cherry selected from cherry and cherry.
  • skin wrinkles refers to fine lines that occur as the skin ages, and may be caused by genes, a decrease in collagen and elastin present in the dermis of the skin, or external environments. Accordingly, the term “skin wrinkle improvement” means suppressing or inhibiting the formation of wrinkles on the skin, or alleviating wrinkles that have already formed.
  • enhancing or improving skin elasticity refers to an increase in skin elasticity, suppressing or inhibiting loss of skin elasticity, or alleviating already reduced elasticity.
  • improvement refers to any action that improves or beneficially changes skin wrinkles or elasticity by administering or treating the composition of the present invention.
  • One or more extracts or fractions thereof selected from the cherries and cherries may be obtained using the extract preparation method described above.
  • One or more extracts or fractions thereof selected from the cherries and cherries may contain collagen.
  • composition may include the extract or fraction thereof as an active ingredient.
  • the term “included as an active ingredient” means that the extract or fraction thereof is added to an extent that can produce the effects mentioned above. Additionally, this may include formulation in various forms by adding various ingredients as sub-ingredients for delivery and stabilization.
  • the formulation of the cosmetic composition is not particularly limited, and for example, any selected from skins, lotions, essences, creams, packs, foundations, patches, hair tonics, micro needle patches, and makeup bases. It may have one dosage form, but may not be limited thereto.
  • the cosmetic composition may contain substances arbitrarily selected to suit the formulation or purpose of use of the cosmetic, but may not be limited thereto.
  • substances arbitrarily selected to suit the formulation or purpose of use of the cosmetic but may not be limited thereto.
  • purified water, oil, surfactant, moisturizer, higher alcohol, viscosity reducing agent, chelating agent, colorant, fatty acid, antioxidant, preservative, wax, pH adjuster, fragrance, etc. may be added, but may not be limited thereto. there is.
  • the carrier ingredients include animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide. It may be possible, but may not be limited thereto.
  • the carrier component may include lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder, and especially, in the case of a spray, chlorofluorohydrocarbon may be added. , propane/butane, or dimethyl ether, but may not be limited thereto.
  • the formulation of the cosmetic composition when it is a solution or emulsion, it may include a solvent, solubilizing agent, or emulsifying agent as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, and benzyl benzoate. It may include, but is not limited to, fatty acid esters of ester, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or sorbitan.
  • the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and miso. It may include, but is not limited to, crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant.
  • the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid. It may include, but is not limited to, amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.
  • Another aspect is to provide a food composition for improving skin wrinkles or improving elasticity, comprising as an active ingredient one or more extracts or fractions thereof selected from cherry and cherry.
  • a food composition for improving skin wrinkles or improving elasticity comprising as an active ingredient one or more extracts or fractions thereof selected from cherry and cherry.
  • extracts or fractions thereof selected from cherry and cherry selected from cherry and cherry.
  • food in this specification refers to meat, sausages, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, and alcoholic beverages. , vitamin complexes, health functional foods, and health foods, etc., and include all foods in the conventional sense.
  • the food can be manufactured by methods commonly used in the industry, and can be manufactured by adding raw materials and ingredients commonly added in the industry. Additionally, the food formulation can be manufactured without limitation as long as it is a formulation recognized as a food.
  • the food composition can be manufactured in various types of formulations, and unlike general drugs, it is made from food as a raw material, has the advantage of not having side effects that may occur when taking the drug for a long time, and is highly portable, so the food composition of the present invention It can be taken as a supplement to improve the effect of bone-related diseases.
  • the food composition may be a health functional food composition.
  • health functional food in this specification refers to food manufactured and processed using raw materials or ingredients with functionality useful to the human body in accordance with Act No. 6727 on Health Functional Food, and “functional” refers to food that is manufactured and processed using functional ingredients useful to the human body. It means controlling the structure and function of nutrients or obtaining useful effects for health purposes such as physiological effects.
  • the above-mentioned health food refers to food that has a more active health maintenance or promotion effect compared to general food
  • health supplement food refers to food for the purpose of supporting health.
  • the terms health functional food, health food, and health supplement may be used interchangeably.
  • the health functional food is a food manufactured by adding the composition to food materials such as beverages, teas, spices, gum, and confectionery, or by encapsulating, powdering, or suspending it, and has specific health effects when consumed.
  • the health functional food is a food manufactured by adding the composition to food materials such as beverages, teas, spices, gum, and confectionery, or by encapsulating, powdering, or suspending it, and has specific health effects when consumed.
  • unlike regular drugs it has the advantage of being made from food and eliminating the side effects that can occur when taking the drug for a long period of time.
  • the food composition can be consumed on a daily basis, it can be expected to be highly effective in improving skin wrinkles and/or improving elasticity, so it can be very useful.
  • the food composition may further include a physiologically acceptable carrier.
  • the type of carrier is not particularly limited and any carrier commonly used in the art can be used.
  • the food composition may include additional ingredients that are commonly used in food compositions to improve smell, taste, vision, etc.
  • it may include vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid, etc.
  • it may contain minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu), and chromium (Cr).
  • it may contain amino acids such as lysine, tryptophan, cysteine, and valine.
  • the food composition contains preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), disinfectants (bleaching powder, high bleaching powder, sodium hypochlorite, etc.), antioxidants (butylhydroxyanisole (BHA), butylhydroxide) roxitoluene (BHT), etc.), colorants (tar color, etc.), coloring agents (sodium nitrite, sodium nitrite, etc.), bleaching agents (sodium sulfite), seasonings (MSG monosodium glutamate, etc.), sweeteners (dulcine, cyclamate, saccharin) , sodium, etc.), flavorings (vanillin, lactones, etc.), leavening agents (alum, D-potassium hydrogen tartrate, etc.), strengtheners, emulsifiers, thickeners (grease), coating agents, gum base agents, anti-foam agents, solvents, improvers, etc.
  • the food composition can be added as is or used together with other foods or food ingredients, and can be used appropriately according to conventional methods.
  • the mixing amount of the active ingredient can be appropriately determined depending on the purpose of use (prevention, health, or therapeutic treatment).
  • the food composition of the present invention may be added in an amount of 50 parts by weight or less, specifically 20 parts by weight or less, relative to the food or beverage.
  • the content when consumed for a long time for health and hygiene purposes, the content may be below the above range. Since there is no problem in terms of safety, the active ingredient may be used in amounts above the above range.
  • the food composition it can be used as a health drink composition, and in this case, it may contain various flavoring agents or natural carbohydrates as additional ingredients, like regular drinks.
  • the above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; polysaccharides such as dextrins and cyclodextrins; It may be a sugar alcohol such as xylitol, sorbitol, or erythritol.
  • Sweeteners include natural sweeteners such as thaumatin and stevia extract; Synthetic sweeteners such as saccharin and aspartame can be used.
  • the ratio of the natural carbohydrate may be generally about 0.01 to 0.04 g, specifically about 0.02 to 0.03 g, per 100 mL of the health drink composition of the present invention.
  • the health drink composition includes various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, It may contain alcohol or carbonating agent. Additionally, it may contain pulp for the production of natural fruit juice, fruit juice beverage, or vegetable beverage. These ingredients can be used independently or in combination. The ratio of these additives is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health drink composition of the present invention.
  • the food composition may contain the extract of the present invention in various weight% if it can exhibit the effect of improving skin wrinkles and/or improving elasticity.
  • the compound may be added in an amount of 0.00001 to 100% by weight or 0.01 to 0.01% by weight based on the total weight of the food composition. It may contain 80% by weight, but is not limited thereto.
  • compositions for topical skin application for improving skin wrinkles or improving elasticity which includes one or more extracts selected from cherry and cherry or a fraction thereof as an active ingredient.
  • extracts selected from cherry and cherry or a fraction thereof as an active ingredient.
  • the skin external composition may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof.
  • a person skilled in the art can appropriately select and mix ingredients other than the composition of the present invention according to the formulation or purpose of use of other external skin preparations. In this case, when applied simultaneously with other raw materials, there is a synergistic effect. can happen.
  • the skin external preparations include ingredients commonly used in external skin preparations such as cosmetics and medicines, such as water-based ingredients, oil-based ingredients, powder ingredients, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, and fragrances. , colorants, various skin nutrients, or a combination thereof may be appropriately mixed according to need.
  • the skin external preparations include metal sequestrants such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, belafamil, licorice extract, glablidin, and calin.
  • metal sequestrants such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, belafamil, licorice extract, glablidin, and calin.
  • Hot water extract of fruit, various herbal medicines, drugs such as tocopherol acetate, glytylitinic acid, tranexamic acid and its derivatives or salts, vitamin C, magnesium ascorbate phosphate, ascorbate glucoside, arbutin, kojic acid, glucose, fructose, Sugars such as trehalose can also be appropriately mixed.
  • Another aspect is to provide a method for improving skin wrinkles or improving elasticity, comprising the step of applying a composition containing as an active ingredient one or more extracts or fractions thereof selected from cherry and cherry to the skin of an individual.
  • a composition containing as an active ingredient one or more extracts or fractions thereof selected from cherry and cherry to the skin of an individual.
  • the “individual” refers to all living organisms, such as rats, mice, livestock, cells, etc., including humans, that can cause skin wrinkles and/or loss of skin elasticity. As a specific example, it may be a mammal, including humans, and includes not only in vivo but also in vitro conditions.
  • the method may be a beauty method through skin improvement.
  • application includes methods of administration or application to the skin of a subject, such as application by fingers, brush, cotton ball, pad, spray, sponge, cotton swab, roll-on, etc. .
  • a person skilled in the art can easily determine the appropriate method to apply.
  • the composition may be a cosmetic composition or a composition for external application to the skin.
  • the applying step may be applying the composition at intervals of 1 minute to 90 minutes, specifically 1 minute to 90 minutes, 5 minutes to 90 minutes, 1 minute to 60 minutes, and 5 minutes to 90 minutes. It may be at intervals of 60 minutes, 1 minute to 30 minutes, 5 minutes to 30 minutes, 10 minutes to 30 minutes, 10 minutes to 90 minutes, or 10 minutes to 60 minutes.
  • the applying step may be applying the composition 1 to 30 times, specifically 1 to 30 times, 1 to 25 times, 5 to 30 times, 5 to 25 times, It may be applied 1 to 20 times, 5 to 20 times, 10 to 20 times, 10 to 30 times, or 10 to 25 times.
  • Another aspect includes 1) grinding a raw material containing collagen using wet grinding (aqua grinding); 2) Obtaining an extract or fraction thereof from the pulverized raw material; and 3) separating collagen from the extract or fraction thereof.
  • wet grinding aqua grinding
  • the step of separating collagen from the extract or fraction thereof may be effectively separating collagen using known techniques.
  • Raw materials containing the collagen include cherry (Prunus Avium), cherry (Prunus Tomentosa), avocado (Persea americana), olive (Olea europaea), carrot (Daucus carota), bean (Glycine max), tomato (Solanum lycopersicum), and blue It may include one or more selected from the group consisting of berries (Vaccinium spp.), and may specifically be cherries or cherry.
  • the amount of collagen produced by the above method may be increased by more than 5% than the amount of collagen produced by a method using dry grinding instead of wet grinding. Specifically, specifically, 5% or more, 8% or more, 10% or more, 15% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, 90 It may be an increase of more than %, more than 99%, or 100%. More specifically 5% to 100%, 5% to 90%, 5% to 80%, 5% to 70%, 5% to 60%, 5% to 50%, 5% to 40%, 5% to 30%.
  • the amount of collagen produced by the above method may be increased by more than 5% than the amount of collagen produced by a method using dry grinding instead of wet grinding. Specifically, specifically, 5% or more, 8% or more, 10% or more, 15% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, 90 It may be an increase of more than %, more than 99%, or 100%. More specifically 5% to 100%, 5% to 90%, 5% to 80%, 5% to 70%, 5% to 60%, 5% to 50%, 5% to 40%, 5% to 30%.
  • Another aspect is to provide a use of one or more extracts or fractions thereof selected from cherry and cherry to improve skin wrinkles or improve elasticity.
  • extracts or fractions thereof selected from cherry and cherry to improve skin wrinkles or improve elasticity.
  • the same parts as described above also apply to the above use.
  • One or more extracts or fractions thereof selected from the cherries and cherries may be obtained using the extract preparation method described above.
  • One or more extracts or fractions thereof selected from the cherries and cherries may contain collagen.
  • collagen production efficiency can be improved by increasing the collagen content in the extract.
  • 1 is a schematic flow diagram of a process including wet grinding.
  • Figure 2 is a diagram showing the results of comparative analysis of the content of collagen components when cherry extract was manufactured using a wet grinding process.
  • Figure 3 is a diagram showing the results of comparative analysis of the content of hydroxyproline according to the performance time of the wet grinding process.
  • Example 1 Collagen-containing extract manufacturing process
  • Example 2 Comparative analysis of energy consumption and carbon dioxide generation of wet grinding-based extract manufacturing process
  • Example 1 the content of cherry collagen components contained in the cherry extract of Example 1 was confirmed through High Performance Liquid Chromatography (HPLC) analysis.
  • HPLC High Performance Liquid Chromatography
  • 1.0 ⁇ l of the extract prepared in Example 1 was injected into the column, and then the solvent was flowed at a flow rate of 2.0 ml/min.
  • the solvent consisted of two mobile phases, 40 mM Phosphate buffer pH 7.8 (solvent A) and methanol/acetonitrile/water (45/45/10, v/v, solvent B), and the concentration gradient was as follows. Conditions were used: 50-100% solvent A. Additionally, the detection wavelengths were 262 nm and 338 nm.
  • the control group extract was tested using the same method as cherry extract prepared using the existing dry grinding process.
  • ingredient Cherry extract obtained by wet grinding process control group (using dry grinding process) Hydroxyproline 210 ⁇ g/kg 190 ⁇ g/kg Proline 505 ⁇ g/kg 397 ⁇ g/kg Glycine 210 ⁇ g/kg 180 ⁇ g/kg
  • the collagen content according to grinding time was compared through the hydroxyproline content to determine the optimal grinding time.
  • the hydroxyproline content tended to increase as the grinding time increased within a certain period of time, and the highest hydroxyproline content was found when grinding for 10 minutes. was detected ( Figure 3). Therefore, in the case of wet grinding, the highest collagen content was confirmed when the grinding time was 10 minutes, and the optimal wet grinding time was established.

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Abstract

La présente invention concerne un procédé de production de collagène ou de préparation d'un extrait de Prunus avium L. ou Prunus tomentosa à l'aide d'un broyage humide, et des utilisations de réduction des rides de la peau et d'amélioration de l'élasticité du collagène ou de l'extrait produit par le procédé. Selon un aspect, il a été identifié que si le procédé de production de collagène et le procédé de préparation d'extrait de Prunus avium L. de la présente invention sont utilisés, la quantité de collagène dans des extraits est augmentée de telle sorte que l'efficacité de production de collagène peut être améliorée.
PCT/KR2023/019719 2022-12-15 2023-12-01 Procédé de production de collagène à l'aide d'un procédé de broyage humide Ceased WO2024128651A1 (fr)

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